[{"awards": "2333917 Dong, Xiaoli", "bounds_geometry": "POLYGON((161 -77.5,161.1 -77.5,161.2 -77.5,161.3 -77.5,161.4 -77.5,161.5 -77.5,161.6 -77.5,161.7 -77.5,161.8 -77.5,161.9 -77.5,162 -77.5,162 -77.51,162 -77.52,162 -77.53,162 -77.53999999999999,162 -77.55,162 -77.56,162 -77.57,162 -77.58,162 -77.58999999999999,162 -77.6,161.9 -77.6,161.8 -77.6,161.7 -77.6,161.6 -77.6,161.5 -77.6,161.4 -77.6,161.3 -77.6,161.2 -77.6,161.1 -77.6,161 -77.6,161 -77.58999999999999,161 -77.58,161 -77.57,161 -77.56,161 -77.55,161 -77.53999999999999,161 -77.53,161 -77.52,161 -77.51,161 -77.5))", "dataset_titles": null, "datasets": null, "date_created": "Thu, 27 Feb 2025 00:00:00 GMT", "description": "Ecosystems worldwide are threatened by anthropogenic changes in climate. Lakes are widely regarded as sentinels of climate change and, among these, polar lakes are the most sensitive. Beneath meters of permanent ice and liquid water, many Antarctic lakes contain complex microbial communities that are already being transformed by climate change. The structurally complex spatial patterns that these microbes create provide the opportunity to pursue research questions about spatial ecology that cannot be addressed elsewhere. This project focuses on research that will advance understanding of the spatial structure of benthic communities in Antarctic lakes, their relationships with environmental conditions, and predictions for likely changes in the future. This project will also advance methods in integrating the morphology and spatial patterning of modern microbial communities in relationship to their biophysical and biochemical environments. The quantitative framework being developed has potential to refine understanding of controls on microbial community patterning and thus interpretation of both the effects of climate change and ancient fossil microbial communities in the geologic record. Such understanding will address key questions about Earth\u2019s evolutionary and environmental history and future. Lake Vanda in the McMurdo Dry Valleys, Antarctic, has modern microbial pinnacles covering its lake floor. Using existing datasets on spatial structure of benthic communities from 37 sites on the floor of Lake Vanda, the project team will apply recent theories from Spatial Ecology to investigate the mechanisms that give rise to spatial patterns of pinnacles formed by benthic microbes. The work addresses two questions: (1) What are the morphological and spatial patterns of pinnacles and how do they vary over developmental stages, along environment gradients, and from 2013 to 2023? And (2) what mechanisms give rise to the geometry of individual pinnacles and their spatial distribution? Lake Vanda provides an exceptional opportunity to address these questions. It features well characterized gradients in sedimentation, nutrients, irradiance, transport mechanism, and colonization history. Benthic communities at different locations in the lake manifest distinct spatial patterns, as they experience distinct conditions. Lake level has increased \u003e10 m in the past few decades, creating additional opportunities for a \u201cnatural experiment\u201d on pattern development by comparing relatively newly flooded substrates (pinnacles of 1 to 15 years old) with deeper, well-developed mats (\u003e 70 years old). Since microbial communities respond to environmental change rapidly, analyses can characterize changes in patterns in pinnacle spatial data collected 9 years apart (Dec 2013 and Jan 2023), providing the opportunity to directly assess responses of spatially self-organized ecosystems to environmental change. As such, Lake Vanda is a natural laboratory that allows research (1) to effectively sort out mechanisms of pattern formation affecting benthic microbial communities residing there; and (2) to test the theory of spatial self-organization: mechanisms of pattern formation and responses to perturbations, applicable to ecosystems worldwide. Research questions will be addressed by integrating existing datasets, spatial pattern analyses, Bayesian statistical models, and process-based numerical models. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 162.0, "geometry": "POINT(161.5 -77.55)", "instruments": null, "is_usap_dc": true, "keywords": "Lake Vanda; ECOLOGICAL DYNAMICS", "locations": "Lake Vanda", "north": -77.5, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Dong, Xiaoli; Sumner, Dawn", "platforms": null, "repositories": null, "science_programs": null, "south": -77.6, "title": "Effects of Environmental Change on Microbial Self-organized Patterns in Antarctic Lakes", "uid": "p0010499", "west": 161.0}, {"awards": "2142914 Baker, Bill; 2142912 Murray, Alison; 2142913 Tresguerres, Martin", "bounds_geometry": "POLYGON((-180 -60,-168 -60,-156 -60,-144 -60,-132 -60,-120 -60,-108 -60,-96 -60,-84 -60,-72 -60,-60 -60,-60 -62,-60 -64,-60 -66,-60 -68,-60 -70,-60 -72,-60 -74,-60 -76,-60 -78,-60 -80,-72 -80,-84 -80,-96 -80,-108 -80,-120 -80,-132 -80,-144 -80,-156 -80,-168 -80,180 -80,178 -80,176 -80,174 -80,172 -80,170 -80,168 -80,166 -80,164 -80,162 -80,160 -80,160 -78,160 -76,160 -74,160 -72,160 -70,160 -68,160 -66,160 -64,160 -62,160 -60,162 -60,164 -60,166 -60,168 -60,170 -60,172 -60,174 -60,176 -60,178 -60,-180 -60))", "dataset_titles": null, "datasets": null, "date_created": "Thu, 17 Oct 2024 00:00:00 GMT", "description": "Non-technical description Marine invertebrates often have mutually beneficial partnerships with microorganisms that biosynthesize compounds with nutritive or defensive functions and are integral for survival. Additionally, these \u201cnatural products\u201d often have bioactive properties with human health applications fighting infection or different types of cancer. This project focuses on the ascidian (\u201csea squirt\u201d) Synoicum adareanum, found in the Anvers Island region of the Antarctic Peninsula, and was recently discovered to contain high levels of a natural product, palmerolide A (palA) in its tissues. The microorganism that produces palA is a new bacterial species, Candidatus Synoicihabitans palmerolidicus, found in a persistent partnership with the sea squirt. There is still much to be learned about the fundamental properties of this sea squirt-microbe-palA system including the geographical range of the animal-microbe partnership, its chemical and microbiome complexity and diversity, and the biological effect of palA in the sea squirt. To address these questions, this multidisciplinary research team will investigate the sea squirt-microbiome partnership in the Antarctic Peninsula and McMurdo Sound regions of the Ross Sea using a state-of-the-art strategy that will advance our understanding of the structural and functional features of the sea squirt and microbiome in detail, and reveal the roles that the palA natural product plays in the host ecology in its native Antarctic seafloor habitat. The project will broaden diversity and provide new opportunities for early career students and postdoctoral researchers to participate in field and laboratory-based research that builds an integrative understanding of Antarctic marine biology, ecology, physiology and chemistry. In addition, advancing the understanding of palA and its biological properties may be of future benefit to biomedicine and human health. Technical description Marine invertebrates and their associated microbiomes can produce bioactive natural products; in fact, \u003e600 such compounds have been identified in species from polar waters. Although such compounds are typically hypothesized to serve ecological roles in host survival through deterring predation, fouling, and microbial infection, in most cases neither the producing organism nor the genome-encoded biosynthetic enzymes are known. This project will study an emerging biosynthetic system from a polar ascidian-microbe association that produces palA, a natural product with bioactivity against the proton-pumping enzyme V-type H+-ATPase (VHA). The objectives include: (i) Determining the microbiome composition, metabolome complexity, palA levels, and mitochondrial DNA sequence of S. adareanum morphotypes at sites in the Antarctic Peninsula and in McMurdo Sound, (ii) Characterizing the Synoicum microbiome using a multi-omics strategy, and (iii) Assessing the potential for co-occurrence of Ca. S. palmerolidicus-palA-VHA in host tissues, and (iv) exploring the role of palA in modulating VHA activity in vivo and its effects on ascidian-microbe ecophysiology. Through a coupled study of palA-producing and non-producing S. adareanum specimens, structural and functional features of the ascidian microbiome metagenome will be characterized to better understand the relationship between predicted secondary metabolite pathways and whether they are expressed in situ using a paired metatranscriptome sequencing and secondary metabolite detection strategy. Combined with tissue co-localization results, functional ecophysiological assays aim to determine the roles that the natural product plays in the host ecology in its native Antarctic seafloor habitat. The contributions of the project will inform this intimate host-microbial association in which the ascidian host bioaccumulates VHA-inhibiting palA, yet its geo-spatial distribution, cellular localization, ecological and physiological role(s) are not known. In addition to elucidating the ecophysiological roles of palA in their native ascidian-microbe association, the results will contribute to the success of translational science, which aligns with NSF\u2019s interests in promoting basic research that leads to advances in Biotechnology and Bioeconomy. The project will also broaden diversity and provide new opportunities for early career students and postdoctoral researchers to participate in field and laboratory-based research that builds an integrative understanding of Antarctic marine biology, ecology, physiology and chemistry. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 160.0, "geometry": "POINT(-130 -70)", "instruments": null, "is_usap_dc": true, "keywords": "ECOSYSTEM FUNCTIONS; BACTERIA/ARCHAEA; BENTHIC; R/V NBP; Antarctic Peninsula; ANIMALS/INVERTEBRATES", "locations": "Antarctic Peninsula", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Baker, Bill; Murray, Alison; Tresguerres, Martin", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repositories": null, "science_programs": null, "south": -80.0, "title": "Collaborative Research: ANT LIA: Diving into the Ecology of an Antarctic Ascidian-Microbiome-Palmerolide Association using a Multi-omic and Functional Approach", "uid": "p0010485", "west": -60.0}, {"awards": "2031121 Junge, Karen", "bounds_geometry": null, "dataset_titles": null, "datasets": null, "date_created": "Tue, 30 Jul 2024 00:00:00 GMT", "description": "The ozone hole that develops over the Antarctic continent every spring is one of the features attributed to human activity, in particular production of the CFC (chlorofluorocarbons in refrigerants) released to the atmosphere. In spite of the CFC ban from the Montreal Protocol established in the year 1987, the recovery has been slower than predicted. Bromocarbons, known to produce the stratospheric ozone depletion, have recently been estimated to contribute to the pool of bromines in the lower atmosphere. What is the origin of the bromocarbons in Antarctic sea ice? Is this an additional source of chemicals creating the ozone hole? This project will test if bromocarbons in sea ice are produced and degraded by microalgae and bacteria found in sea ice, in snow and the interface between the two. The project will collect a suite of chemical and biological measurements of sea ice and snow to determine bromocarbon concentrations, microbial activity associated with them, and intra-cellular genes and proteins involved in bromocarbon metabolism. This project benefits NSF\u2019s goals of expanding fundamental knowledge of Antarctic systems, biota, and processes, and improving the understanding of interactions among the Antarctic systems, cryosphere and organisms. The work will be carried out at McMurdo Station in late austral spring, including sampling of snow and ice that will be concentrated in the laboratory, and 24-hour experiments to measure algal and bacterial activity. Genes controlling synthesis of enzymes involved in bromocarbon metabolism are of interest in biotechnology and bioremediation, including products that repair damaged skin from UltraViolet Radiation. The project will train undergraduate students on chemical and biological techniques. The Principal Investigators will be involved in the Pacific Science Center in Seattle with ~10,000 visitors per weekend where they will develop a project-specific exhibit. The microbial processes in snow and ice associated with bromocarbon synthesis and degradation have not been studied in Antarctica during winter and spring. This study will inform about microbial activity in relation to the release of bromocarbons compounds from the snow and ice surfaces, compounds known to degrade stratospheric ozone. The estimation of chemical bromocarbons will be combined with metagenomics and metaproteomics approaches for understanding the potential role of microbes in snow and sea ice. The environmental, chemical and biological data will be synthesized with multivariate analysis and significant differences between sites and experimental treatments with ANOVA. A collaborator from the University of Goteborg in Sweden will collaborate on bromocarbon analyses. The study will also address \u201csaline snow\u201d a new environment not previously studied for microbial life. In addition, this is the first study of meta-proteomics in snow and ice. The Principal Investigators expect their results will help inform ozone hole recovery in the 21st century. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "McMurdo Sound; COASTAL; BACTERIA/ARCHAEA; SEA ICE; SNOW/ICE", "locations": "McMurdo Sound", "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Junge, Karen; Nunn, Brook L", "platforms": null, "repositories": null, "science_programs": null, "south": null, "title": "Sea-ice Snow Microbial Communities\u2019 Impact on Antarctic Bromocarbon Budgets and Processes", "uid": "p0010472", "west": null}, {"awards": "2336354 Juarez Rivera, Marisol", "bounds_geometry": "POLYGON((160 -76.5,160.45 -76.5,160.9 -76.5,161.35 -76.5,161.8 -76.5,162.25 -76.5,162.7 -76.5,163.15 -76.5,163.6 -76.5,164.05 -76.5,164.5 -76.5,164.5 -76.7,164.5 -76.9,164.5 -77.1,164.5 -77.3,164.5 -77.5,164.5 -77.7,164.5 -77.9,164.5 -78.1,164.5 -78.3,164.5 -78.5,164.05 -78.5,163.6 -78.5,163.15 -78.5,162.7 -78.5,162.25 -78.5,161.8 -78.5,161.35 -78.5,160.9 -78.5,160.45 -78.5,160 -78.5,160 -78.3,160 -78.1,160 -77.9,160 -77.7,160 -77.5,160 -77.3,160 -77.1,160 -76.9,160 -76.7,160 -76.5))", "dataset_titles": "Lake Fryxell 2022-2023 benthic microbial mat thickness and number of laminae", "datasets": [{"dataset_uid": "601839", "doi": "10.15784/601839", "keywords": "Antarctica; Cryosphere; Dry Valleys; Lake Fryxell; Laminae; Microbial Mat; Thickness", "people": "Mackey, Tyler; Juarez Rivera, Marisol; Sumner, Dawn; Paul, Ann; Hawes, Ian", "repository": "USAP-DC", "science_program": null, "title": "Lake Fryxell 2022-2023 benthic microbial mat thickness and number of laminae", "url": "https://www.usap-dc.org/view/dataset/601839"}], "date_created": "Fri, 05 Jul 2024 00:00:00 GMT", "description": "Perennially ice-covered lakes in the McMurdo Dry Valleys of Antarctica contain abundant microbial mats, and the export of this mat material can fertilize the surrounding polar desert ecosystems. These desert soils are one of the most organic-poor on earth yet host a community of microorganisms. Microbial mat material is exported from the shallow, gas-supersaturated regions of the lakes when gas bubbles form in the mats, lifting them to the ice cover; the perennial ice cover maintains gas supersaturation. These mats freeze in and are exported to the surrounding soils through ice ablation. The largest seasonal decrease and thinnest ice cover in the history of Lake Fryxell was recorded during the 2022-2023 Austral summer. In this thin ice year, the water column dissolved oxygen increased over prior observations, and the lake bottom surface area with bubble-disrupted mat was more than double that observed in 1980-1981 and 2006-2007. This work will constrain mat mobilization within and out of Lake Fryxell in the McMurdo Dry Valleys during a period of unprecedented ice thinning to understand how future changing regional climate and predicted seasonal loss of lake ice cover will affect nutrient transport in the McMurdo Dry Valleys. Exceptional years of mat export are hypothesized to have the most significant impact on nutrient export to soil communities; variability in mat liftoff may thus play a role in the McMurdo Dry Valleys ecosystem response to changing climate. The perennial ice cover of lakes in the McMurdo Dry Valleys of Antarctica modulates the transfer of gasses, organic and inorganic material, between the lakes and surrounding soils. The export of biomass in these lakes is driven by the supersaturation of atmospheric gasses in the shallow regions under perennial ice cover. Gas bubbles nucleate in the mats, producing buoyancy that lifts them to the bottom of the ice, where they freeze in and are exported to the surrounding soils through ice ablation. These mats represent a significant source of biomass and nutrients to the McMurdo Dry Valleys soils, which are among the most organic-poor on earth. Nevertheless, this biomass remains unaccounted for in organic carbon cycling models for the McMurdo Dry Valleys. Ice cover data from the McMurdo Dry Valleys Long Term Ecological Research Project shows that the ice thickness has undergone cyclical variation over the last 40 years, reaching the largest seasonal decrease and thinnest ice-cover in the recorded history of Lake Fryxell during the 2022-2023 austral summer. Preliminary work shows that the surface area with mat liftoff at Lake Fryxell is more than double that observed in 1980-1981 and 2006-2007, coinciding with this unprecedented thinning of the ice-cover and an increase in the water column dissolved O2. This research will constrain biomass mobilization within and out of Lake Fryxell in the McMurdo Dry Valleys during a period of unprecedented ice thinning. The researchers hypothesize that a thinner ice cover promotes more biomass mobilization by 1) stimulating additional production of gas bubbles from the existing gas-supersaturated waters during summertime photosynthesis to create microbial mat liftoff and 2) promoting mat liftoff in deeper, thicker microbial mats, and 3) that this biomass can be traced into the soils by characterizing its chemistry and modeling the most likely depositional settings. This work will use microbial mat samples, lake dissolved oxygen and photosynthetically active radiation data and underwater drone footage documenting the depth distribution of liftoff mats in January 2023, and long-term ice cover thickness, photosynthetically active radiation, and lake level change data collected by the McMurdo Dry Valleys Long Term Ecological Research Project to test hypotheses 1-3. The dispersal of the liftoff mat exposed at Lake Fryxell surface will be modeled using a Hybrid Single-Particle Lagrangian Integrated Trajectory (HYSPLIT) model. Exceptional liftoff years like the present are hypothesized to have the most significant impact on the soil communities as the rates of soil respiration increase with the addition of carbon. However, continued warming in the next 10 - 40 years may result in seasonal loss of the ice cover and cessation of liftoff mat export. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 164.5, "geometry": "POINT(162.25 -77.5)", "instruments": null, "is_usap_dc": true, "keywords": "Stable Isotopes; MINERALS; LAKE/POND; ISOTOPES; Organic Matter; McMurdo Dry Valleys; SEDIMENTARY ROCKS", "locations": "McMurdo Dry Valleys", "north": -76.5, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Earth Sciences", "paleo_time": null, "persons": "Juarez Rivera, Marisol", "platforms": null, "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -78.5, "title": "RAPID: Is Biomass Mobilization at Ice-covered Lake Fryxell, Antarctica reaching a Critical Threshold?", "uid": "p0010467", "west": 160.0}, {"awards": "1903681 Brook, Edward J.", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "Final N2O isotopic data including isotopomer ratios for the last deglaciation and Heinrich Stadia 4/Dansgaard Oeschger Event 8; Preliminary nitrous oxide site preference isotopic data for last deglaciation from Taylor Glacier", "datasets": [{"dataset_uid": "601592", "doi": "10.15784/601592", "keywords": "Antarctica; Nitrous Oxide; Taylor Glacier", "people": "Menking, Andy; Brook, Edward J.", "repository": "USAP-DC", "science_program": null, "title": "Preliminary nitrous oxide site preference isotopic data for last deglaciation from Taylor Glacier", "url": "https://www.usap-dc.org/view/dataset/601592"}, {"dataset_uid": "601803", "doi": "10.15784/601803", "keywords": "Antarctica; Cryosphere; Ice Core; Nitrous Oxide; Taylor Glacier", "people": "Menking, Andy; Brook, Edward J.", "repository": "USAP-DC", "science_program": null, "title": "Final N2O isotopic data including isotopomer ratios for the last deglaciation and Heinrich Stadia 4/Dansgaard Oeschger Event 8", "url": "https://www.usap-dc.org/view/dataset/601803"}], "date_created": "Wed, 19 Jun 2024 00:00:00 GMT", "description": "The objective of this project is to understand why the nitrous oxide (N2O) content of the atmosphere was lower during the last ice age (about 20,000-100,000 years ago) than in the subsequent warm period (10,000 years ago to present) and why it fluctuated during climate changes within the ice age. Nitrous oxide is a greenhouse gas that contributes to modern global warming. It is thought that modern warming will in turn cause increases in natural sources of nitrous oxide from bacteria in soils and the ocean, creating a \"positive feedback.\" However, the amount these sources will increase is uncertain because the different ways that nitrous oxide are produced, and how sensitive they are to warmer climate, are not well known. This project will measure a unique property of the nitrous oxide molecule in very large ancient air samples from a glacier in Antarctica. This method can distinguish between different microbial processes that produce nitrous oxide but it has not been applied yet to the time periods in question. The data will provide information about how natural climate changes affect nitrous oxide production. This, in turn, will be useful for predicting future changes and for understanding why the Earth\u0027s climate shifts from ice ages to warm periods and back again. Ice-core records of greenhouse gas isotopic composition are useful for determining past changes in natural source and sink strengths and for understanding how natural emissions are linked to climate change. This project will develop two records of the intramolecular site preference of Nitrogen-15 in N2O. One record spans the last deglaciation (10,000-21,000 years ago) when atmospheric N2O concentration rose by 30 percent, and the other record spans millennial-scale climate changes during the last ice age when N2O varied by smaller amounts (Heinrich Stadial 4 and Dansgaard Oeschger 8, 35,000-41,000 years ago). The records will be used to understand what changes in the nitrogen cycle caused atmospheric N2O concentration to vary and what mechanisms link the N2O emissions to climate change. Ideally, studying the two different time periods will isolate the millennial climate responses entangled with the full deglacial sequence, creating a clearer picture of how N2O biogeochemistry responds to climate change. This work will also allow exploration of an isotopic tracer for in situ production of N2O that contaminates the atmospheric signal in particularly dusty ice. The project will use a unique, well-dated suite of ice samples from Taylor Glacier, Antarctica and continuous flow isotope ratio mass spectrometry on a custom gas extraction line operated in the Oregon State University laboratory. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "Taylor Glacier; Nitrous Oxide; TRACE GASES/TRACE SPECIES; Ice Core; Stable Isotopes; NITROUS OXIDE", "locations": "Taylor Glacier", "north": -60.0, "nsf_funding_programs": "Antarctic Glaciology; Antarctic Earth Sciences", "paleo_time": "PHANEROZOIC \u003e CENOZOIC \u003e QUATERNARY", "persons": "Brook, Edward", "platforms": null, "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -90.0, "title": "Deciphering Changes in Atmospheric Nitrous Oxide Concentration During the Last Ice Age Using the Intramolecular Site-Preference of Nitrogen Isotopes", "uid": "p0010465", "west": -180.0}, {"awards": "2325922 Couradeau, Estelle", "bounds_geometry": "POLYGON((-73.783 4.679,-73.7827 4.679,-73.7824 4.679,-73.7821 4.679,-73.7818 4.679,-73.7815 4.679,-73.7812 4.679,-73.7809 4.679,-73.7806 4.679,-73.7803 4.679,-73.78 4.679,-73.78 4.6789,-73.78 4.6788,-73.78 4.6787,-73.78 4.6786,-73.78 4.6785,-73.78 4.6784,-73.78 4.6783,-73.78 4.6782,-73.78 4.6781,-73.78 4.678,-73.7803 4.678,-73.7806 4.678,-73.7809 4.678,-73.7812 4.678,-73.7815 4.678,-73.7818 4.678,-73.7821 4.678,-73.7824 4.678,-73.7827 4.678,-73.783 4.678,-73.783 4.6781,-73.783 4.6782,-73.783 4.6783,-73.783 4.6784,-73.783 4.6785,-73.783 4.6786,-73.783 4.6787,-73.783 4.6788,-73.783 4.6789,-73.783 4.679))", "dataset_titles": null, "datasets": null, "date_created": "Tue, 06 Feb 2024 00:00:00 GMT", "description": "P\u00e1ramos are high-altitude tundra ecosystems nested at the heart of the Andes mountains. These cold and humid environments are home to a multitude of plants, animals, and insects. P\u00e1ramos are a critical water source for downstream urban centers, including Colombia\u0027s capital city, Bogota. Additionally, the P\u00e1ramos soils contain substantial organic carbon reserves due to slow rates of organic matter decomposition. Beyond being a pool of carbon sequestered away from the atmosphere, this large reservoir of organic matter controls the soils\u2019 hydraulic and fertility properties. The P\u00e1ramos\u2019 unique geographic location, at an elevation above 2,800 m above sea level, makes them highly vulnerable to the impacts of climate change. In fact, these ecosystems\u2019 surface areas are projected to shrink by half within the next 50 years possibly causing loss of the essential services they provide. This project aims to characterize the microbial diversity in the P\u00e1ramos soils in Colombia and investigate how climate change will affect microbes\u2019 functions. The research is of high importance, considering that immediate and long-term changes in microbial metabolism could impact the ability of P\u00e1ramos soils to store organic carbon and regulate downstream water flow. To study the cascading effect of climate change on P\u00e1ramos ecosystems, this project will jumpstart collaborations among transdisciplinary experts that will integrate the research of below-ground microbial communities with above-ground vegetation functions. The project will also engage high school and undergraduate students that will work together to develop and deploy low-cost long-term soil monitoring data loggers in Chingaza National Natural Park, near the city of Bogota. This project will address the critical need to disentangle the effect of moisture and temperature on the fate of organic carbon in P\u00e1ramos soils while building a transdisciplinary team capable of expanding the scope of the research to an ecosystem level. The project includes establishing controlled soil mesocosms that will allow to independently vary moisture and temperature levels. Additionally, functions of the soil microbiome will be investigated using metagenomics and amplicon sequencing, and probes will be deployed to initiate long-term monitoring of the soil response to climate change in situ. This project will culminate in the organization of an international P\u00e1ramos symposium that will set up priorities for future systems research. The symposium will bring together scientists from diverse fields to discuss the linkages between above-ground and below-ground ecosystem functions and plan future collaborations in predicting P\u00e1ramos-wide effects of climate change. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": -73.78, "geometry": "POINT(-73.7815 4.6785)", "instruments": null, "is_usap_dc": true, "keywords": "TERRESTRIAL ECOSYSTEMS; Chingaza Paramos Colombia; ORGANIC MATTER; SOIL MECHANICS", "locations": "Chingaza Paramos Colombia", "north": 4.679, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Couradeau, Estelle; Maximova, Siela; Machado, Jose Luis", "platforms": null, "repositories": null, "science_programs": null, "south": 4.678, "title": "Collaborative Research: BoCP-Design: Climate change alteration of soils functional biodiversity of the P\u00e1ramos, Colombia", "uid": "p0010445", "west": -73.783}, {"awards": "2224760 Gooseff, Michael", "bounds_geometry": "POINT(162.87 -77)", "dataset_titles": "EDI Data Portal: McMurdo Dry Valleys LTER", "datasets": [{"dataset_uid": "200379", "doi": "", "keywords": null, "people": null, "repository": "Environmental Data Initiative (EDI)", "science_program": null, "title": "EDI Data Portal: McMurdo Dry Valleys LTER", "url": "https://portal.edirepository.org/nis/browseServlet?searchValue=MCM"}], "date_created": "Tue, 14 Nov 2023 00:00:00 GMT", "description": "Non-technical Abstract The McMurdo Dry Valleys LTER seeks to understand how changes in the temporal variability of ecological connectivity interact with existing landscape legacies to alter the structure and functioning of this extreme polar desert ecosystem. This research has broad implications, as it will help us to understand how natural ecosystems respond to ongoing anthropogenic global change. At the same time, this project also serves an important educational and outreach function, providing immersive research and educational experiences to students and artists from diverse backgrounds, and helping to ensure a diverse and well-trained next generation of leaders in polar ecosystem science and stewardship. Ultimately, the results of this project will help us to better understand and prepare for the effects of climate change and develop scientific insights that are relevant far beyond Antarctic ecosystems. The McMurdo Dry Valleys (MDVs) make up an extreme polar desert ecosystem in the largest ice-free region of Antarctica. The organisms in this ecosystem are generally small. Bacteria, microinvertebrates, cyanobacterial mats, and phytoplankton can be found across the streams, soils, glaciers, and ice-covered lakes. These organisms have adapted to the cold and arid conditions that prevail outside of lakes for all but a brief period in the austral summer when the ecosystem is connected by liquid water. In the summer when air temperatures rise barely above freezing, soils warm and glacial meltwater flows through streams into the open moats of lakes. Most biological activity across the landscape occurs in summer. Through the winter, or polar night (6 months of darkness), glaciers, streams, and soil biota are inactive until sufficient light, heat, and liquid water return, while lake communities remain active all year. Over the past 30 years, the MDVs have been disturbed by cooling, heatwaves, floods, rising lake levels, as well as permafrost and lake ice thaw. Considering the clear ecological responses to this variation in physical drivers, and climate models predicting further warming and more precipitation, the MDV ecosystem sits at a threshold between the current extreme cold and dry conditions and an uncertain future. This project seeks to determine how important the legacy of past events and conditions versus current physical and biological interactions shape the current ecosystem. Four hypotheses will be tested, related to 1) whether the status of specific organisms are indicative ecosystem stability, 2) the relationship between legacies of past events to current ecosystem resilience (resistance to big changes), 3) carryover of materials between times of high ecosystem connectivity and activity help to maintain ecosystem stability, and 4) changes in disturbances affect how this ecosystem persists through the annual polar night (i.e., extended period of dark and cold). Technical Abstract In this iteration of the McMurdo LTER project (MCM6), the project team will test ecological connectivity and stability theory in a system subject to strong physical drivers (geological legacies, extreme seasonality, and contemporary climate change) and driven by microbial organisms. Since microorganisms regulate most of the world\u2019s critical biogeochemical functions, these insights will be relevant far beyond polar ecosystems and will inform understanding and expectations of how natural and managed ecosystems respond to ongoing anthropogenic global change. MCM6 builds on previous foundational research, both in Antarctica and within the LTER network, to consider the temporal aspects of connectivity and how it relates to ecosystem stability. The project will examine how changes in the temporal variability of ecological connectivity interact with the legacies of the existing landscape that have defined habitats and biogeochemical cycling for millennia. The project team hypothesizes that the structure and functioning of the MDV ecosystem is dependent upon legacies and the contemporary frequency, duration, and magnitude of ecological connectivity. This hypothesis will be tested with new and continuing monitoring, experiments, and analyses of long-term datasets to examine: 1) the stability of these ecosystems as reflected by sentinel taxa, 2) the relationship between ecological legacies and ecosystem resilience, 3) the importance of material carryover during periods of low connectivity to maintaining biological activity and community stability, and 4) how changes in disturbance dynamics disrupt ecological cycles through the polar night. Tests of these hypotheses will occur in field and modeling activities using new and long-term datasets already collected. New datasets resulting from field activities will be made freely available via widely-known online databases (MCM LTER and EDI). The project team has also developed six Antarctic Core Ideas that encompass themes from data literacy to polar food webs and form a consistent thread across the education and outreach activities. Building on past success, collaborations will be established with teachers and artists embedded within the science teams, who will work to develop educational modules with science content informed by direct experience and artistic expression. Undergraduate mentoring efforts will incorporate computational methods through a new data-intensive scientific training program for MCM REU students. The project will also establish an Antarctic Research Experience for Community College Students at CU Boulder, to provide an immersive educational and research experience for students from diverse backgrounds in community colleges. MCM LTER will continue its mission of training and mentoring students, postdocs, and early career scientists as the next generation of leaders in polar ecosystem science and stewardship. Historically underrepresented participation will be expanded at each level of the project. To aid in these efforts, the project has established Education \u0026 Outreach and Diversity, Equity, and Inclusion committees to lead, coordinate, support, and integrate these activities through all aspects of MCM6. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 162.87, "geometry": "POINT(162.87 -77)", "instruments": null, "is_usap_dc": true, "keywords": "COMMUNITY DYNAMICS; ABLATION ZONES/ACCUMULATION ZONES; SOIL TEMPERATURE; DIATOMS; FIELD INVESTIGATION; PERMANENT LAND SITES; BUOYS; GROUND-BASED OBSERVATIONS; SEDIMENTS; SNOW WATER EQUIVALENT; SPECIES/POPULATION INTERACTIONS; WATER-BASED PLATFORMS; FIXED OBSERVATION STATIONS; VIRUSES; PHYTOPLANKTON; ACTIVE LAYER; FIELD SURVEYS; RADIO TRANSMITTERS; DATA COLLECTIONS; ECOLOGICAL DYNAMICS; LANDSCAPE; GROUND WATER; SNOW/ICE CHEMISTRY; LAND-BASED PLATFORMS; ANIMALS/INVERTEBRATES; ECOSYSTEM FUNCTIONS; HUMIDITY; GEOCHEMISTRY; SURFACE WINDS; RIVERS/STREAM; GLACIER MASS BALANCE/ICE SHEET MASS BALANCE; SNOW; LAND RECORDS; ATMOSPHERIC PRESSURE; SURFACE TEMPERATURE; ATMOSPHERIC RADIATION; BACTERIA/ARCHAEA; AIR TEMPERATURE; GLACIERS; SNOW/ICE TEMPERATURE; SOIL CHEMISTRY; METEOROLOGICAL STATIONS; WATER QUALITY/WATER CHEMISTRY; TERRESTRIAL ECOSYSTEMS; MOORED; PROTISTS; STREAMFLOW STATION; Dry Valleys; LAKE/POND; LAKE ICE; SNOW DEPTH; AQUATIC ECOSYSTEMS; SNOW DENSITY; FIELD SITES", "locations": "Dry Valleys", "north": -77.0, "nsf_funding_programs": "Antarctic Integrated System Science; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Gooseff, Michael N.; Adams, Byron; Barrett, John; Diaz, Melisa A.; Doran, Peter; Dugan, Hilary A.; Mackey, Tyler; Morgan-Kiss, Rachael; Salvatore, Mark; Takacs-Vesbach, Cristina; Zeglin, Lydia H.", "platforms": "LAND-BASED PLATFORMS; LAND-BASED PLATFORMS \u003e FIELD SITES; LAND-BASED PLATFORMS \u003e FIELD SITES \u003e DATA COLLECTIONS; LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION; LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS; LAND-BASED PLATFORMS \u003e FIELD SITES \u003e RADIO TRANSMITTERS; LAND-BASED PLATFORMS \u003e PERMANENT LAND SITES; LAND-BASED PLATFORMS \u003e PERMANENT LAND SITES \u003e FIXED OBSERVATION STATIONS; LAND-BASED PLATFORMS \u003e PERMANENT LAND SITES \u003e GROUND-BASED OBSERVATIONS; LAND-BASED PLATFORMS \u003e PERMANENT LAND SITES \u003e METEOROLOGICAL STATIONS; LAND-BASED PLATFORMS \u003e PERMANENT LAND SITES \u003e STREAMFLOW STATION; WATER-BASED PLATFORMS; WATER-BASED PLATFORMS \u003e BUOYS; WATER-BASED PLATFORMS \u003e BUOYS \u003e MOORED; WATER-BASED PLATFORMS \u003e BUOYS \u003e MOORED \u003e BUOYS", "repo": "Environmental Data Initiative (EDI)", "repositories": "Environmental Data Initiative (EDI)", "science_programs": "LTER", "south": -77.0, "title": "LTER: MCM6 - The Roles of Legacy and Ecological Connectivity in a Polar Desert Ecosystem", "uid": "p0010440", "west": 162.87}, {"awards": "2142491 Young, Jodi", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": null, "datasets": null, "date_created": "Wed, 26 Jul 2023 00:00:00 GMT", "description": "Sea ice in Antarctic coastal waters shape ecosystems, both in the surface waters and at the bottom of the ocean, environments that depend on algae living in sea ice for their productivity. With high variability in sea ice formation and melt between years and as a response to climate change, it is of importance to obtain better understanding of the interaction of sea ice with algae, as well as provide better data for global climate models. This project will accomplish those goals by measuring phytoplankton growth and cellular properties in sea ice with experiments performed using an ice tank. Laboratory experiments will be based on previous observations in the Antarctic Peninsula coastal waters, providing realistic conditions to emulate. The scientific importance of the proposed work aligns with the National Science Foundation goals to understand the biological and chemical properties of sea ice bio-geo-chemistry and its feedbacks with seasonal sea ice dynamics and climate. The finding from this project will be of interest to a broad scientific community, including oceanographers, biologists, chemists, and ecosystem and ocean modelers. To address the scarcity of data on sea ice microbes that limits our ability to predict future Antarctic climate with accuracy, the principal investigator will develop an Antarctic Science Minor in order to train future scientists with an environmental perspective and prepare the future US workforce with a strong scientific background on Earth and Biological Sciences. There is a paucity of data to understand the processes underlying observed patters in sea ice quality and their interaction with the sea-ice microbial community. This project will provide a mechanistic understanding of primary production and physiology of sympagic algae over the seasonal cycle of formation and melt of Antarctic sea ice. Although sea ice is central to the Antarctic coastal ecosystems, little is known of how they affect, and are in turn affected, by sea-ice algae. This project concentrates on first-year sea ice, forming and melting each year, creating unique and very dynamic habitats. The study will be structured by 4 main objectives: 1) how different algal species adapt to the seasonal changes in sea ice conditions, 2) how different methods to measure primary production (carbon dioxide drawdown, oxygen production and variable fluorescence) relate in sea ice and differ from sea water measurements, 3) how sympagic algae influence the physical structure of sea ice, 4) how sympagic algae contribute to organic matter cycling during ice melt. Due to expected changes in sea ice due to climate change, this study is uniquely positioned to provide needed data on short-term and seasonal processes. Results from this study will be useful to refine models of algal production in Antarctic and Arctic ecosystems, data not available to date as sea ice and its biogeochemistry are often poorly represented in earth system models. This project will also provide education for graduate and undergraduate students as well as material to develop class curriculum for middle-school students. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "MARINE ECOSYSTEMS", "locations": null, "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Ocean and Atmospheric Sciences", "paleo_time": null, "persons": "Young, Jodi", "platforms": null, "repositories": null, "science_programs": null, "south": -90.0, "title": "CAREER: Experimentally Testing the Role of Sympagic Algae in Sea-ice Environments using a Laboratory Scale Ice-tank.", "uid": "p0010425", "west": -180.0}, {"awards": "2228257 Michaud, Alexander", "bounds_geometry": "POINT(-112.05 -79.28)", "dataset_titles": null, "datasets": null, "date_created": "Wed, 31 May 2023 00:00:00 GMT", "description": "Ice cores from glaciers and ice sheets provide detailed archives of past environmental conditions, furthering our understanding of Earth\u2019s climate. Microorganisms in the West Antarctic Ice Sheet are buried over glaciological time and form a stratigraphy record providing the opportunity of analysis of the order and position of layers of geological events, with potential links to Southern Hemisphere climate. However, microbial cells that land on the ice sheet are subject to the stresses of changing habitat conditions due to burial and conditions associated with long-term isolation in ice. These processes may lead to a loss of fidelity within the stratigraphic record of microbial cells. We know little about how and if microorganisms survive burial and remain alive over glacial-interglacial time periods within an ice sheet. This analysis will identify the viable and preserved community of microorganisms and core genomic adaptation that permit cell viability, which will advance knowledge in the areas of microbiology and glaciology while increasing fidelity of ice core measurements relevant to past climate and potential future global climate impacts. This exploratory endeavor has the potential to be a transformative step toward understanding the ecology of one of the most understudied environments on Earth. The project will partner with the Museum of Science, Boston, to increase public scientific literacy via education and outreach. Additionally, this project will support two early-career scientists and two undergraduates in interdisciplinary research at the intersection of microbiology and climate science. Results from this project will provide the first DNA data based on single-cell whole genomic sequencing from the Antarctic Ice Sheet and inform whether post-depositional processes impact the interpretations of paleoenvironmental conditions from microbes. The goals to determine the taxonomic identity of viable and preserved microbial cells, and decode the genetic repertoire that confers survival of burial and long-term viability within glacial ice, will be achieved by utilizing subsamples from a ~60,000 year old record of the West Antarctic Ice Sheet Divide (WD) Ice Core. WD samples will be melted using the Desert Research Institute\u2019s ice core melting system that is optimized for glaciobiological sampling. Microbial cells from the meltwater will be sorted using fluorescence-activated cell sorting, and individually sorted cells will have their genomes sequenced. The fluorescence-based methods will discern the viable (metabolically active) cells from those cells that are non-viable but preserved in the ice (DNA-containing). The genomic analysis will identify the taxonomy of each cell, presence of known genes that confer survival in permanently frozen environments, and comparatively analyze genomes to determine the core set of genes required by viable cells to persist in an ice sheet. The outcomes of this work will expand the potential for biological measurements and contamination control from archived ice cores. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": -112.05, "geometry": "POINT(-112.05 -79.28)", "instruments": null, "is_usap_dc": true, "keywords": "WAIS Divide; TERRESTRIAL ECOSYSTEMS; ICE SHEETS; BACTERIA/ARCHAEA; ICE CORE RECORDS", "locations": "WAIS Divide", "north": -79.28, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Michaud, Alexander; Winski, Dominic A.", "platforms": null, "repositories": null, "science_programs": null, "south": -79.28, "title": "EAGER: ANT LIA: Persist or Perish: Records of Microbial Survival and Long-term Persistence from the West Antarctic Ice Sheet", "uid": "p0010421", "west": -112.05}, {"awards": "2137378 Varsani, Arvind; 2137377 Bergstrom, Anna; 2137376 Porazinska, Dorota; 2137375 Schmidt, Steven", "bounds_geometry": null, "dataset_titles": null, "datasets": null, "date_created": "Wed, 10 May 2023 00:00:00 GMT", "description": "Cryoconite holes are sediment-filled melt holes in the surface of glaciers that can be important sites of active microbial life in an otherwise mostly frozen and barren landscape. Previous studies in the McMurdo Dry Valleys, Antarctica suggest that viral infections of microbes, and a general lack of fertilizers (i.e., nutrients), may be important factors shaping the development and functioning of microbial communities in cryoconite holes. The researchers propose an experimental approach to understand how nutrient limitation affects diversity (number of species) and overall abundance of microbes, and how the diversity and abundance of microbes in turn affects the diversity, abundance, and infection type of viruses that parasitize the microbes in cryoconite sediments. The researchers will use sediments previously collected from Antarctic glaciers that have varying concentrations of viruses and nutrients, to set up a nutrient-addition experiment to determine how nutrients affect microbial and viral population dynamics. The results will deepen our understanding of how microbial communities in general are shaped by nutrients and viruses and give new insights into the functioning of viruses in extremely cold environments. The researchers will publish their findings in scientific journals and will share their discoveries with K-12 students from rural schools in collaboration with the Pinhead Institute and will connect undergraduate students from under-represented minorities to polar research through participation in the university\u2019s Science, Technology, Engineering \u0026 Mathematics Routes Uplift Research Program. Outreach will be achieved through videos produced and distributed by a professional science communicator. The research advances a National Science Foundation goal of expanding fundamental knowledge of Antarctic systems, biota, and processes by utilizing the unique characteristics of the Antarctic region as a science observing platform. The Principal Investigators propose an experimental approach to understand how nutrient limitation affects microbial diversity and abundances and their cascading effects on virus diversity, abundance, and mode of infection (lysis vs. lysogeny) in Antarctic cryoconite holes. Cryoconite holes are ideal natural microcosms for manipulative studies, not available in other cryospheric ecosystems. The PIs will use previously collected cryoconite from across a gradient of both viral diversity and nutrient levels to address questions about key limiting nutrients and microbial-viral community dynamics in cryoconite sediments. Nutrient manipulation experiments will be conducted in a growth chamber that closely approximates the light and temperature regime of in situ cryoconite holes to test three core hypotheses: (1) phosphorus availability limits microbial productivity and abundance in cryoconite holes; (2) relaxing nutrient limitation in cryoconite from low-diversity glaciers will increase species diversity, leading microbial communities to resemble those found on more nutrient-rich glaciers; (3) relaxing nutrient limitation will increase the diversity and abundance of viruses by increasing the availability of suitable hosts, and decrease the prevalence of lysogenic infections. By manipulating nutrient limitation within a realistic range, this project will help verify hypothesized phosphorus limitation of Antarctic cryoconite holes and will extend understanding of the connections between nutrients, diversity, and viral infection dynamics in the cryosphere more generally. A better understanding of these dynamics in cryoconite sediments improves the ability of scientists to forecast future impacts of environmental changes in the cryosphere. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "AQUATIC ECOSYSTEMS; Taylor Valley", "locations": "Taylor Valley", "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Varsani, Arvind; Porazinska, Dorota; Schmidt, Steven; Bergstrom, Anna", "platforms": null, "repositories": null, "science_programs": null, "south": null, "title": "Collaborative Research: Role of Nutrient Limitation and Viral Interactions on Antarctic Microbial Community Assembly: A Cryoconite Microcosm Study", "uid": "p0010418", "west": null}, {"awards": "2133684 Fierer, Noah", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "Geochemical, physicochemical, and genomic data from a continental-scale survey of microbial diversity in Antarctic soils", "datasets": [{"dataset_uid": "200478", "doi": "10.6073/pasta/b4858653c587864f0111aba4c3014d61", "keywords": null, "people": null, "repository": "EDI", "science_program": null, "title": "Geochemical, physicochemical, and genomic data from a continental-scale survey of microbial diversity in Antarctic soils", "url": "https://portal.edirepository.org/nis/mapbrowse?packageid=knb-lter-mcm.275.1"}], "date_created": "Fri, 07 Apr 2023 00:00:00 GMT", "description": "Not all of Antarctica is covered in ice. In fact, soils are common to many parts of Antarctica, and these soils are often unlike any others found on Earth. Antarctic soils harbor unique microorganisms able to cope with the extremely cold and dry conditions common to much of the continent. For decades, microbiologists have been drawn to the unique soils in Antarctica, yet critical knowledge gaps remain. Most notably, it is unclear what properties allow certain microbes to thrive in Antarctic soils. By using a range of methods, this project is developing comprehensive model that discovers the unique genomic features of soils diversity, distributions, and adaptations that allow Antarctic soil microbes to thrive in extreme environments. The proposed work will be relevant to researchers in many fields, including engineers seeking to develop new biotechnologies, ecologists studying the contributions of these microbial communities to the functioning of Antarctic ecosystems, microbiologists studying novel microbial adaptations to extreme environmental conditions, and even astrobiologists studying the potential for life on Mars. More generally, the proposed research presents an opportunity to advance our current understanding of the microbial life found in one of the more distinctive microbial habitats on Earth, a habitat that is inaccessible to many scientists and a habitat that is increasingly under threat from climate change. The research project explores the microbial diversity in Antarctic soils and links specific features to different soil types and environmental conditions. The overarching questions include: What microbial taxa are found in a variety of Antarctic environments? What are the environmental preferences of specific taxa or lineages? What are the genomic and phenotypic traits of microorganisms that allow them to persist in extreme environments and determine biogeographical differneces? This project will analyze archived soils collected from across Antarctica by a network of international collaborators, with samples selected to span broad gradients in soil and site conditions. The project uses cultivation-independent, high-throughput genomic analysis methods and cultivation-dependent approaches to analyze bacterial and fungal communities in soil samples. The results will be used to predict the distributions of specific taxa and lineages, obtain genomic information for the more ubiquitous and abundant taxa, and quantify growth responses in vitro across gradients in temperature, moisture, and salinity. This integration of ecological, environmental, genomic, and trait-based information will provide a comprehensive understanding of microbial life in Antarctic soils. This project will also help facilitate new collaborations between scientists across the globe while providing undergraduate students with \u0027hands-on\u0027 research experiences that introduce the next generation of scientists to the field of Antarctic biology. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "FUNGI; BACTERIA/ARCHAEA; TERRESTRIAL ECOSYSTEMS; Antarctica", "locations": "Antarctica", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Fierer, Noah; Quandt, Alisha A; Lemonte, Joshua", "platforms": null, "repo": "EDI", "repositories": "EDI", "science_programs": null, "south": -90.0, "title": "Collaborative Research: ANT LIA Integrating Genomic and Phenotypic Analyses to understand Microbial Life in Antarctic Soils", "uid": "p0010414", "west": -180.0}, {"awards": "2037963 Smith, Heidi", "bounds_geometry": null, "dataset_titles": null, "datasets": null, "date_created": "Tue, 11 Oct 2022 00:00:00 GMT", "description": "Glacial ice cores serve as a museum back in time, providing detailed records of past climatic conditions. In addition to chronological records such as temperature, chemistry and gas composition, ice provides a unique environment for preserving microbes and other biological materials through time. These microbes provide invaluable insight into the physiological capabilities necessary for survival in the Earth\u2019s cryosphere and other icy planetary bodies, yet little is known about them. This award supports fundamental research into the activity of microbes in ice, and directly supports major research priorities regarding Antarctic biota identified in the 2015 National Academies of Sciences, Engineering, and Medicine report, A Strategic Vision for NSF Investments in Antarctic and Southern Ocean Research. The broader impacts of this work are that it will be relevant to researchers across paleoclimate and biological fields. It will support two early career researchers, a graduate and an undergraduate student who will conduct laboratory analyses, participate in outreach activities, publish papers in scientific journals and present at conferences. This work will use previously collected ice cores to investigate englacial microbial activity from the Holocene back to the Last Glacial Maximum from the blue ice area of Taylor Glacier, Antarctica. The proposal identified making significant contributions to 1) investing how Antarctic organisms evolve and adapt to changing environment, 2) understanding how microbes alter the preservation of paleorecord-relevant gas and trace element information in ice cores, and 3) identifying microbial life in cores and their activity in relation to dust depositional events. Two recently developed complementary techniques (bio-orthogonal noncanonical amino acid tagging and deuterium isotope probing) in combination with Raman Confocal Microspectroscopy will be used to assess and quantify microbial activity in ice. During phase one of the project, these methods will be optimized using deaccessioned ice cores available at the National Science Foundation\u2019s Ice Core Facility. In phase two, ice cores in a time series from the Taylor Glacier will be analyzed for geochemistry and microbial activity. Research results will provide a comprehensive view of englacial microbial communities, including their metabolic diversity and activity, and the effect of geochemical parameters on microbial assemblages from different climate periods. Given the dearth of information available on englacial microbial communities, the results of this research will be of particular significance. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "Microbial Activity; LABORATORY; Paleoclimate; CAMP; Taylor Glacier; Microbiology; Alaska; ICE CORE RECORDS", "locations": "CAMP; Alaska; Taylor Glacier", "north": null, "nsf_funding_programs": "Antarctic Integrated System Science", "paleo_time": null, "persons": "Smith, Heidi; Foreman, Christine; Dieser, Markus", "platforms": "OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repositories": null, "science_programs": null, "south": null, "title": "Life in Ice: Probing Microbial Englacial Activity through Time", "uid": "p0010385", "west": null}, {"awards": "2132641 Bik, Holly", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -62,180 -64,180 -66,180 -68,180 -70,180 -72,180 -74,180 -76,180 -78,180 -80,144 -80,108 -80,72 -80,36 -80,0 -80,-36 -80,-72 -80,-108 -80,-144 -80,-180 -80,-180 -78,-180 -76,-180 -74,-180 -72,-180 -70,-180 -68,-180 -66,-180 -64,-180 -62,-180 -60))", "dataset_titles": null, "datasets": null, "date_created": "Tue, 30 Aug 2022 00:00:00 GMT", "description": "Non-technical Abstract: The long isolation and unique biodiversity of the Southern Ocean represents an important case study region for understanding the evolution and ecology of populations. This study uses modern -omics approaches to evaluate the biodiversity, evolution, and ecology of Antarctic marine nematodes and their host-associated microbiomes from a variety of habitats collected at different depths. The results are producing an important baseline dataset of Antarctic meiofaunal diversity. All genomic resources generated in this project will be publicly accessible as open-source datasets with the potential for long-term scientific reuse. This project supports diverse researchers from underrepresented backgrounds and produces a suite of Antarctic-focused digital public outreach products. Technical Abstract: Nematode worms are abundant and ubiquitous in marine sediment habitats worldwide, performing key functions such as nutrient cycling and sediment stability. However, study of this phylum suffers from a perpetual and severe taxonomic deficit, with less than 5,000 formally described marine species. Fauna from the Southern Ocean are especially poorly studied due to limited sampling and the general inaccessibility of the Antarctic benthos. This study is providing the first large-scale molecular-based investigation from marine nematodes in the Eastern Antarctic continental shelf, providing an important comparative dataset for the existing body of historical (morphological) taxonomic studies. This project uses a combination of classical taxonomy (microscopy) and modern -omics tools to achieve three overarching aims: 1) determine if molecular data supports high biodiversity and endemism of benthic meiofauna in Antarctic benthic ecosystems; 2) determine the proportion of marine nematode species that have a deep-sea versus shallow-water evolutionary origin on the Antarctic shelf, and assess patterns of cryptic speciation in the Southern Ocean; and 3) determine the most important drivers of the host-associated microbiome in Antarctic marine nematodes. This project is designed to rapidly advance knowledge of the evolutionary origins of Antarctic meiofauna, provide insight on population-level patterns within key indicator genera, and elucidate the potential ecological and environmental factors which may influence microbiome patterns. Broader Impacts activities include an intensive cruise- and land-based outreach program focusing on social media engagement and digital outreach products, raising awareness of Antarctic marine ecosystems and understudied microbial-animal relationships. The diverse research team includes female scientists, first-generation college students, and Latinx trainees. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "East Antarctica; BENTHIC", "locations": "East Antarctica", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Bik, Holly", "platforms": null, "repositories": null, "science_programs": null, "south": -80.0, "title": "ANT LIA: Do Molecular Data Support High Endemism and Divergent Evolution of Antarctic Marine Nematodes and their Host-associated Microbiomes?", "uid": "p0010372", "west": -180.0}, {"awards": "2147045 Learman, Deric", "bounds_geometry": "POLYGON((-180 -60,-168 -60,-156 -60,-144 -60,-132 -60,-120 -60,-108 -60,-96 -60,-84 -60,-72 -60,-60 -60,-60 -62,-60 -64,-60 -66,-60 -68,-60 -70,-60 -72,-60 -74,-60 -76,-60 -78,-60 -80,-72 -80,-84 -80,-96 -80,-108 -80,-120 -80,-132 -80,-144 -80,-156 -80,-168 -80,180 -80,171 -80,162 -80,153 -80,144 -80,135 -80,126 -80,117 -80,108 -80,99 -80,90 -80,90 -78,90 -76,90 -74,90 -72,90 -70,90 -68,90 -66,90 -64,90 -62,90 -60,99 -60,108 -60,117 -60,126 -60,135 -60,144 -60,153 -60,162 -60,171 -60,-180 -60))", "dataset_titles": "Physical and geochemical data from shelf sediments eastern Antarctica", "datasets": [{"dataset_uid": "601876", "doi": "10.15784/601876", "keywords": "Antarctica; Cryosphere", "people": "Learman, Deric", "repository": "USAP-DC", "science_program": null, "title": "Physical and geochemical data from shelf sediments eastern Antarctica", "url": "https://www.usap-dc.org/view/dataset/601876"}], "date_created": "Tue, 30 Aug 2022 00:00:00 GMT", "description": "Microbes in Antarctic surface marine sediments have an important role in degrading organic matter and releasing nutrients to the ocean. Organic matter degradation is at the center of the carbon cycle in the ocean, providing valuable information on nutrient recycling, food availability to animals and carbon dioxide release to the atmosphere. The functionality of these microbes has been inferred by their genomics, however these methods only address the possible function, not their actual rates. In this project the PIs plan to combine genomics methods with cellular estimates of enzyme abundance and activity as a way to determine the rates of carbon degradation. This project aims to sample in several regions of Antarctica to provide a large-scale picture of the processes under study and understand the importance of microbial community composition and environmental factors, such as primary productivity, have on microbial activity. The proposed work will combine research tools such as metagenomics, meta-transcriptomics, and metabolomics coupled with chemical data and enzyme assays to establish degradation of organic matter in Antarctic sediments. This project benefits NSFs goals of understanding the adaptation of Antarctic organisms to the cold and isolated environment, critical to predict effects of climate change to polar organisms, as well as contribute to our knowledge of how Antarctic organisms have adapted to this environment. Society will benefit from this project by education of 2 graduate students, undergraduates and K-12 students as well as increase public literacy through short videos production shared in YouTube. The PIs propose to advance understanding of polar microbial community function, by measuring enzyme and gene function of complex organic matter degradation in several ocean regions, providing a circum-Antarctic description of sediment processes. Two hypotheses are proposed. The first hypothesis states that many genes for the degradation of complex organic matter will be shared in sediments throughout a sampling transect and that where variations in gene content occur, it will reflect differences in the quantity and quality of organic matter, not regional variability. The second hypothesis states that a fraction of gene transcripts for organic matter degradation will not result in measurable enzyme activity due to post-translational modification or rapid degradation of the enzymes. The PIs will analyze sediment cores already collected in a 2020 cruise to the western Antarctic Peninsula with the additional request of participating in a cruise in 2023 to East Antarctica. The PIs will analyze sediments for metagenomics, meta-transcriptomics, and metabolomics coupled with geochemical data and enzyme assays to establish microbial degradation of complex organic matter in Antarctic sediments. Organic carbon concentrations and content in sediments will be measured with \u03b413C, \u03b415N, TOC porewater fluorescence in bulk organic carbon. Combined with determination of geographical variability as well as dependence on carbon sources, results from this study could provide the basis for new hypotheses on how climate variability, with increased water temperature, affects geochemistry in the Southern Ocean. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 90.0, "geometry": "POINT(-165 -70)", "instruments": null, "is_usap_dc": true, "keywords": "BENTHIC; ECOSYSTEM FUNCTIONS; Weddell Sea; Antarctic Peninsula; SEDIMENT CHEMISTRY; R/V NBP", "locations": "Antarctic Peninsula; Weddell Sea", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Learman, Deric", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -80.0, "title": "Collaborative Research: ANT LIA: Connecting Metagenome Potential to Microbial Function: Investigating Microbial Degradation of Complex Organic Matter Antarctic Benthic Sediments", "uid": "p0010373", "west": -60.0}, {"awards": "1937546 Morgan-Kiss, Rachael; 1937595 Briggs, Brandon", "bounds_geometry": "POLYGON((162 -77.616667,162.1 -77.616667,162.2 -77.616667,162.3 -77.616667,162.4 -77.616667,162.5 -77.616667,162.6 -77.616667,162.7 -77.616667,162.8 -77.616667,162.9 -77.616667,163 -77.616667,163 -77.6283336,163 -77.6400002,163 -77.6516668,163 -77.6633334,163 -77.67500000000001,163 -77.68666660000001,163 -77.69833320000001,163 -77.7099998,163 -77.7216664,163 -77.733333,162.9 -77.733333,162.8 -77.733333,162.7 -77.733333,162.6 -77.733333,162.5 -77.733333,162.4 -77.733333,162.3 -77.733333,162.2 -77.733333,162.1 -77.733333,162 -77.733333,162 -77.7216664,162 -77.7099998,162 -77.69833320000001,162 -77.68666660000001,162 -77.67500000000001,162 -77.6633334,162 -77.6516668,162 -77.6400002,162 -77.6283336,162 -77.616667))", "dataset_titles": "18S rRNA from McMurdo Dry Valley lakes", "datasets": [{"dataset_uid": "200436", "doi": "", "keywords": null, "people": null, "repository": "NCBI SRA", "science_program": null, "title": "18S rRNA from McMurdo Dry Valley lakes", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1125919/"}], "date_created": "Wed, 27 Jul 2022 00:00:00 GMT", "description": "Part I: Non-technical description: Microbial communities are of more than just a scientific curiosity. Microbes represent the single largest source of evolutionary and biochemical diversity on the planet. They are the major agents for cycling carbon, nitrogen, phosphorus, and other elements through the ecosystem. Despite their importance in ecosystem function, microbes are still generally overlooked in food web models and nutrient cycles. Moreover, microbes do not live in isolation: their growth and metabolism are influenced by complex interactions with other microorganisms. This project will focus on the ecology, activity and roles of microbial communities in Antarctic Lake ecosystems. The team will characterize the genetic underpinnings of microbial interactions and the influence of environmental gradients (e.g. light, nutrients, oxygen, sulfur) and seasons (e.g. summer vs. winter) on microbial networks in Lake Fryxell and Lake Bonney in the Taylor Valley within the McMurdo Dry Valley region. Finally, the project furthers the NSF goals of training new generations of scientists by including undergraduate and graduate students, a postdoctoral researcher and a middle school teacher in both lab and field research activities. This partnership will involve a number of other outreach training activities, including visits to classrooms and community events, participation in social media platforms, and webinars. Part II: Technical description: Ecosystem function in the extreme Antarctic Dry Valleys ecosystem is dependent on complex biogeochemical interactions between physiochemical environmental factors (e.g. light, nutrients, oxygen, sulfur), time of year (e.g. summer vs. winter) and microbes. Microbial network complexity can vary in relation to specific abiotic factors, which has important implications on the fragility and resilience of ecosystems under threat of environmental change. This project will evaluate the influence of biogeochemical factors on microbial interactions and network complexity in two Antarctic ice-covered lakes. The study will be structured by three main objectives: 1) infer positive and negative interactions from rich spatial and temporal datasets and investigate the influence of biogeochemical gradients on microbial network complexity using a variety of molecular approaches; 2) directly observe interactions among microbial eukaryotes and their partners using flow cytometry, single-cell sorting and microscopy; and 3) develop metabolic models of specific interactions using metagenomics. Outcomes from amplicon sequencing, meta-omics, and single-cell genomic approaches will be integrated to map specific microbial network complexity and define the role of interactions and metabolic activity onto trends in limnological biogeochemistry in different seasons. These studies will be essential to determine the relationship between network complexity and future climate conditions. Undergraduate researchers will be recruited from both an REU program with a track record of attracting underrepresented minorities and two minority-serving institutions. To further increase polar literacy training and educational impacts, the field team will include a teacher as part of a collaboration with the successful NSF-funded PolarTREC program and participation in activities designed for public outreach. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 163.0, "geometry": "POINT(162.5 -77.67500000000001)", "instruments": null, "is_usap_dc": true, "keywords": "MICROALGAE; AQUATIC ECOSYSTEMS; Antarctica; LAKE/POND; BACTERIA/ARCHAEA; COMMUNITY DYNAMICS", "locations": "Antarctica", "north": -77.616667, "nsf_funding_programs": "Antarctic Integrated System Science; Antarctic Integrated System Science", "paleo_time": null, "persons": "Morgan-Kiss, Rachael; Briggs, Brandon", "platforms": null, "repo": "NCBI SRA", "repositories": "NCBI SRA", "science_programs": null, "south": -77.733333, "title": "ANT LIA: Collaborative Research: Genetic Underpinnings of Microbial Interactions in Chemically Stratified Antarctic Lakes", "uid": "p0010355", "west": 162.0}, {"awards": "1744767 Sanders, Robert", "bounds_geometry": "POLYGON((-68 -64,-67.4 -64,-66.8 -64,-66.2 -64,-65.6 -64,-65 -64,-64.4 -64,-63.8 -64,-63.2 -64,-62.6 -64,-62 -64,-62 -64.5,-62 -65,-62 -65.5,-62 -66,-62 -66.5,-62 -67,-62 -67.5,-62 -68,-62 -68.5,-62 -69,-62.6 -69,-63.2 -69,-63.8 -69,-64.4 -69,-65 -69,-65.6 -69,-66.2 -69,-66.8 -69,-67.4 -69,-68 -69,-68 -68.5,-68 -68,-68 -67.5,-68 -67,-68 -66.5,-68 -66,-68 -65.5,-68 -65,-68 -64.5,-68 -64))", "dataset_titles": "Companion datasets to Diversity of microbial eukaryotes along the West Antarctic peninsula in austral spring.; Expedition Data of NBP1910; Expedition Data of NBP 2205; LMG1904 expedition data; NBP1910_protist_community_RNA Raw sequence reads; NBP2205_protist_community_RNA Raw sequence reads will be made available here after processing is completed", "datasets": [{"dataset_uid": "200325", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data of NBP1910", "url": "https://www.rvdata.us/search/cruise/NBP1910"}, {"dataset_uid": "200365", "doi": "", "keywords": null, "people": null, "repository": "NCBI", "science_program": null, "title": "NBP1910_protist_community_RNA Raw sequence reads; NBP2205_protist_community_RNA Raw sequence reads will be made available here after processing is completed", "url": "https://www.ncbi.nlm.nih.gov/search/all/?term=PRJNA807326"}, {"dataset_uid": "200320", "doi": "10.6084/m9.figshare.19514110.v3", "keywords": null, "people": null, "repository": "Figshare", "science_program": null, "title": "Companion datasets to Diversity of microbial eukaryotes along the West Antarctic peninsula in austral spring.", "url": "https://doi.org/10.6084/m9.figshare.19514110.v3"}, {"dataset_uid": "200366", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data of NBP 2205", "url": "https://www.rvdata.us/search/cruise/NBP2205"}, {"dataset_uid": "200147", "doi": "10.7284/908260", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "LMG1904 expedition data", "url": "https://www.rvdata.us/search/cruise/LMG1904"}], "date_created": "Wed, 27 Jul 2022 00:00:00 GMT", "description": "Traditional models of oceanic food chains have consisted of photosynthetic algae (phytoplankton) being ingested by small animals (zooplankton), which were ingested by larger animals (fish). These traditional models changed as new methods allowed recognition of the importance of bacteria and other non-photosynthetic protozoa in more complex food webs. More recently, the wide-spread existence of mixotrophs (organisms that can both photosynthesize and ingest food particles) and their importance as microbial predators has been recognized in many oceanographic areas. In the Southern Ocean, the only two surveys of mixotrophs have suggested that there may be seasonal differences in their importance as predators. During the long polar night (winter), the ability of mixotrophs to ingest particulate food may aid in their survival thus ensuring a sufficient population in spring to support a phytoplankton bloom once photosynthesis rates can increase. Thus mixotrophs may provide a critical early food source upon which zooplankton and larger animals depend on for growth and reproduction. This project will advance understanding of mixotroph diversity and their ecological impact within the Southern Ocean microbial food web. Specifically, efforts will be focused on mixotrophy in the western Antarctica peninsula region during the austral spring and autumn when there are likely to be changes in the relative importance of photosynthesis and ingestion to mixotrophs. The project will provide research opportunities for undergraduate and graduate students and a post-doctoral researcher. There will be real-time outreach from the Southern Ocean to the public via blogs and interviews, and to high school art students through an established program that blends science and art education. Despite traditional views of protists as either \"phototrophic\" or \"heterotrophic,\" there are many photosynthetic protists that consume prey (mixotrophy). Mixotrophy is a widespread phenomenon in aquatic systems and phytoplankton groups with known mixotrophic species, notably chrysophytes, cryptophytes, prymnesiophytes, prasinophytes and dinoflagellates, are present and often abundant in Antarctic waters. However, in the Southern Ocean, the presence of mixotrophic phytoflagellates has been surveyed only twice: in the Ross Sea during Austral spring 2008 and summer 2011. The primary goals of the project are to gain better understanding of mixotroph diversity and their ecological impact with respect to the Southern Ocean microbial food web. The contribution of mixotrophs to primary production and bacterial consumption is likely linked to the taxonomic composition of the community and the abundance of particular species. Abundances of novel mixotrophic species will be evaluated via qPCR, which will be coupled with assessments of rates of feeding and photosynthesis with the goal of describing how active mixotrophs direct the movement of carbon through food webs. These experiments will help the determination of how viable and widespread mixotrophy is as a nutritional strategy in polar waters and give direct information on the currently unknown diversity of mixotrophic taxa under different environmental conditions occurring in austral spring and autumn. Furthermore, the methods will simultaneously yield information on the whole communities of protists - mixotrophic, phototrophic and heterotrophic. In addition, a method to examine aspects of the taxonomic and functional diversities of the bacterivorous/mixotrophic community will be employed. A thymidine analog (BrdU) will be used to label DNA of eukaryotes feeding on bacteria. The BrdU-labeled eukaryotic DNA will be isolated using immunoprecipitation. High-throughput sequencing of the labeled DNA (bacterivores) versus unlabeled community DNA will determine the diversity of bacterivorous mixotrophs relative to other microeukaryotes. Flow cytometric sorting based on chlorophyll to focus on mixotrophic species. These approaches will elucidate a gap in current knowledge of the influence of microbial interactions in the Southern Ocean under different conditions. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": -62.0, "geometry": "POINT(-65 -66.5)", "instruments": null, "is_usap_dc": true, "keywords": "Antarctic Peninsula; PLANKTON; COASTAL", "locations": "Antarctic Peninsula", "north": -64.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Sanders, Robert; Gast, Rebecca; Jeffrey, Wade H.", "platforms": null, "repo": "R2R", "repositories": "Figshare; NCBI; R2R", "science_programs": null, "south": -69.0, "title": "Collaborative Research: Diversity and ecological impacts of Antarctic mixotrophic phytoplankton", "uid": "p0010357", "west": -68.0}, {"awards": "2055455 Duhaime, Melissa", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": null, "datasets": null, "date_created": "Fri, 03 Jun 2022 00:00:00 GMT", "description": "Part 1: Non-technical description: It is well known that the Southern Ocean plays an important role in global carbon cycling and also receives a disproportionately large influence of climate change. The role of marine viruses on ocean productivity is largely understudied, especially in this global region. This team proposes to use combination of genomics, flow cytometry, and network modeling to test the hypothesis that viral biogeography, infection networks, and viral impacts on microbial metabolism can explain variations in net community production (NCP) and carbon cycling in the Southern Ocean. The project includes the training of a postdoctoral scholar, graduate students and undergraduate students. It also includes the development of a new Polar Sci ReachOut program in partnership with the University of Michigan Museum of Natural History especially targeted to middle-school students and teachers and the general public. The team will also produce a Science for Tomorrow (SFT) program for use in middle schools in metro-Detroit communities and lead a summer Research Experience for Teachers (RET) fellows. Part 2: Technical description: The study will leverage hundreds of existing samples collected for microbes and viruses from the Antarctic Circumpolar Expedition (ACE). These samples provide the first contiguous survey of viral diversity and microbial communities around Antarctica. Viral networks are being studied in the context of biogeochemical data to model community networks and predict net community production (NCP), which will provide a way to evaluate the role of viruses in Southern Ocean carbon cycling. Using cutting edge molecular and flow cytometry approaches, this project addresses the following questions: 1) How/why are Southern Ocean viral populations distributed across environmental gradients? 2a) Do viruses interfere with \"keystone\" metabolic pathways and biogeochemical processes of microbial communities in the Southern Ocean? 2b) Does nutrient availability or other environmental variables drive changes in virus-microbe infection networks in the Southern Ocean? Results will be used to develop and evaluate generative models of NCP predictions that incorporate the importance of viral traits and virus-host interactions. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "Southern Ocean; Amd/Us; AMD; FIELD INVESTIGATION; USA/NSF; AQUATIC SCIENCES; BACTERIA/ARCHAEA; MARINE ECOSYSTEMS; VIRUSES; USAP-DC", "locations": "Southern Ocean", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Duhaime, Melissa; Zaman, Luis", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION", "repositories": null, "science_programs": null, "south": -90.0, "title": "ANT LIA - Viral Ecogenomics of the Southern Ocean: Unifying Omics and Ecological Networks to Advance our Understanding of Antarctic Microbial Ecosystem Function", "uid": "p0010333", "west": -180.0}, {"awards": "1341429 Ball, Becky", "bounds_geometry": "POLYGON((-68.205783 -60.706633,-65.9444531 -60.706633,-63.6831232 -60.706633,-61.4217933 -60.706633,-59.1604634 -60.706633,-56.8991335 -60.706633,-54.6378036 -60.706633,-52.3764737 -60.706633,-50.1151438 -60.706633,-47.8538139 -60.706633,-45.592484 -60.706633,-45.592484 -62.1204014,-45.592484 -63.5341698,-45.592484 -64.9479382,-45.592484 -66.3617066,-45.592484 -67.775475,-45.592484 -69.1892434,-45.592484 -70.6030118,-45.592484 -72.0167802,-45.592484 -73.4305486,-45.592484 -74.844317,-47.8538139 -74.844317,-50.1151438 -74.844317,-52.3764737 -74.844317,-54.6378036 -74.844317,-56.8991335 -74.844317,-59.1604634 -74.844317,-61.4217933 -74.844317,-63.6831232 -74.844317,-65.9444531 -74.844317,-68.205783 -74.844317,-68.205783 -73.4305486,-68.205783 -72.0167802,-68.205783 -70.6030118,-68.205783 -69.1892434,-68.205783 -67.775475,-68.205783 -66.3617066,-68.205783 -64.9479382,-68.205783 -63.5341698,-68.205783 -62.1204014,-68.205783 -60.706633))", "dataset_titles": "Climatic and environmental constraints on aboveground-belowground linkages and diversity across a latitudinal gradient in Antarctica", "datasets": [{"dataset_uid": "200289", "doi": "", "keywords": null, "people": null, "repository": "OSF - Center for Open Science", "science_program": null, "title": "Climatic and environmental constraints on aboveground-belowground linkages and diversity across a latitudinal gradient in Antarctica", "url": "https://osf.io/8xfrc/"}], "date_created": "Thu, 14 Apr 2022 00:00:00 GMT", "description": "The Antarctic Peninsula is experiencing rapid environmental changes, which will influence the community of organisms that live there. However, we know very little about the microscopic organisms living in the soil in this region. Soil biology (including bacteria, fungi, and invertebrates) are responsible for many important processes that sustain ecosystems, such as nutrient recycling. Without understanding the environmental conditions that influence soil biodiversity along the Antarctic Peninsula, our ability to predict the consequences of global change is strongly limited. This project will identify the soil community at many sites along the Antarctic Peninsula to discover how the community changes with environmental conditions from north to south. The project will also identify how the soil community at each site differs under different types of plants. Understanding more about the ways in which plant cover and climate conditions influence soil biodiversity will allow predictions of how communities will respond to future changes such as climate warming and invasive plant species. The project will also further the NSF goals of making scientific discoveries available to the general public and of training new generations of scientists. The investigators will engage with outreach to K-12 students and the general public both directly and through a blog and will participate in workshops for K-12 teachers. Additionally, the project will provide the opportunity for many undergraduate and graduate students of diverse backgrounds to be trained in interdisciplinary research. The investigators will determine the nature and strength of plant-soil linkages in influencing soil community composition and diversity over a latitudinal gradient of environmental and climatic conditions. The goals are to (1) increase our understanding of current biogeography and diversity by providing in-depth knowledge of soil community composition and complexity as it relates to environmental and climatic characteristics; and (2) determine the nature of aboveground-belowground community linkages over varying spatial scales. The team will identify the composition and diversity of soil communities under key habitat types (grass, moss, algae, etc.). Microbial communities (bacteria, fungi, archaea) will be investigated using pyrosequencing for community composition analysis and metagenomic sequencing to identify functional capabilities. Invertebrates (nematodes, tardigrades, rotifers, microarthropods) will be extracted and identified to the lowest possible taxonomic level. Soil chemistry (pH, nutrient content, soil moisture, etc.) and climate conditions will be measured to determine the relationship between soil communities and physical and chemical properties. Structural equation modeling will be used to identify aboveground-belowground linkage pathways and quantify link strengths under varying environmental conditions.", "east": -45.592484, "geometry": "POINT(-56.8991335 -67.775475)", "instruments": null, "is_usap_dc": true, "keywords": "FUNGI; FIELD INVESTIGATION; AMD; Amd/Us; TERRESTRIAL ECOSYSTEMS; USA/NSF; ANIMALS/INVERTEBRATES; SOIL CHEMISTRY; BACTERIA/ARCHAEA; Antarctic Peninsula; ECOSYSTEM FUNCTIONS; USAP-DC", "locations": "Antarctic Peninsula", "north": -60.706633, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Ball, Becky; Van Horn, David", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION", "repo": "OSF - Center for Open Science", "repositories": "OSF - Center for Open Science", "science_programs": null, "south": -74.844317, "title": "Collaborative Research: Climatic and Environmental Constraints on Aboveground-Belowground Linkages and Diversity across a Latitudinal Gradient in Antarctica", "uid": "p0010314", "west": -68.205783}, {"awards": "1932876 Ball, Becky", "bounds_geometry": "POLYGON((-59.666116 -62.15,-59.5128377 -62.15,-59.3595594 -62.15,-59.2062811 -62.15,-59.0530028 -62.15,-58.8997245 -62.15,-58.7464462 -62.15,-58.5931679 -62.15,-58.4398896 -62.15,-58.2866113 -62.15,-58.133333 -62.15,-58.133333 -62.1731502,-58.133333 -62.1963004,-58.133333 -62.2194506,-58.133333 -62.2426008,-58.133333 -62.265751,-58.133333 -62.2889012,-58.133333 -62.3120514,-58.133333 -62.3352016,-58.133333 -62.3583518,-58.133333 -62.381502,-58.2866113 -62.381502,-58.4398896 -62.381502,-58.5931679 -62.381502,-58.7464462 -62.381502,-58.8997245 -62.381502,-59.0530028 -62.381502,-59.2062811 -62.381502,-59.3595594 -62.381502,-59.5128377 -62.381502,-59.666116 -62.381502,-59.666116 -62.3583518,-59.666116 -62.3352016,-59.666116 -62.3120514,-59.666116 -62.2889012,-59.666116 -62.265751,-59.666116 -62.2426008,-59.666116 -62.2194506,-59.666116 -62.1963004,-59.666116 -62.1731502,-59.666116 -62.15))", "dataset_titles": null, "datasets": null, "date_created": "Thu, 14 Apr 2022 00:00:00 GMT", "description": "Part I: Non-technical summary The Antarctic Peninsula warmed very rapidly in the late part of the 20th century, much faster than the global average, and this warming is predicted to resume and continue over the rest of the 21st century. One consequence of this rapid warming is the melting and subsequent retreat of glaciers, leading to an increase in newly-exposed land on the Peninsula that was previously covered with ice. Once new terrain is exposed, the process of ecological succession begins, with the arrival of early-colonizing plants, such as moss and lichens, and soil organisms - a process commonly referred to as the \u201cgreening\u201d of Antarctica. Early stages of succession will be an increasingly common feature on the Antarctic Peninsula, but the mechanisms by which they occur on the Antarctic continent is not well understood. Once the plants have established on the newly-exposed soil, they can change many important properties, such as water dynamics, nutrient recycling, soil development, and habitat for microscopic organisms, which will ultimately determine the structure and functioning of the future ecosystem as it develops. These relationships between vegetation, soil, and the associated microorganisms, referred to as \u201cplant-soil\u201d interactions, are something we know virtually nothing about. This project will be the first to make a comprehensive study of how the type of colonizing plant, and the expansion of those plants from climate change, will influence terrestrial ecosystems in Antarctica. Understanding these processes is critical to understanding how the greening Antarctica is occurring and how soil communities and processes are influenced by these expanding plant communities. Through this work the research team, will also be intensively training undergraduate and graduate students, including training of students from underrepresented groups and collaborative training of students from Chile and the US. Additionally, the research groups will continue their focus on scientific outreach with K-12 schools and the general public to expand awareness of the effects of climate change in Antarctica. Part II: Technical summary In this study, the researchers will use surveys across succession sites along the Antarctic Peninsula and Scotia Arc as well as a manipulative field experiment at glacier succession sites to test how species-specific plant functional traits impact soil properties and associated microbial and invertebrate communities in a greening Antarctica. In doing so, they will pursue three integrated aims to understand how Antarctic plant functional traits alter their soil environment and soil communities during succession after glacial retreat. AIM 1) Characterize six fundamental plant functional traits (thermal conductivity, water holding capacity, albedo, decomposability, tissue nutrient content, and secondary chemistry) among diverse Antarctica flora; AIM 2) Measure the relative effects of fundamental plant functional traits on soil physical properties and soil biogeochemistry across glacial succession gradients in Antarctica; and AIM 3) Measure the relative effects of fundamental plant functional traits on soil microbial and invertebrate communities across glacial succession gradients in Antarctica. They will explore how early-colonizing plants, especially mosses and lichens, alter soil physical, biogeochemical, and biological components, potentially impacting later patterns of succession. The researhcers will use intensive surveys of plant-soil interactions across succession sites and a manipulative transplant experiment in the South Shetland Islands, Antarctica to address their aims. The investigators will collect data on plant functional traits and their effects on soil physical properties, biogeochemistry, biotic abundance, and microbial metagenomics. The data collected will be the first comprehensive measures of the relative importance of plant functional types during glacial retreat and vegetative expansion from climate change in Antarctica, aiding our understanding of how plant functional group diversity and abundance are changing in a greening Antarctica. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": -58.133333, "geometry": "POINT(-58.8997245 -62.265751)", "instruments": null, "is_usap_dc": true, "keywords": "AMD; FIELD SURVEYS; ECOLOGICAL DYNAMICS; USA/NSF; SOIL CHEMISTRY; 25 De Mayo/King George Island; Antarctic Peninsula; PLANTS; Amd/Us; FUNGI; ANIMALS/INVERTEBRATES; USAP-DC; TERRESTRIAL ECOSYSTEMS; BACTERIA/ARCHAEA", "locations": "25 De Mayo/King George Island; Antarctic Peninsula", "north": -62.15, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Ball, Becky", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS", "repositories": null, "science_programs": null, "south": -62.381502, "title": "Collaborative Research: Exploring the Functional Role of Antarctic Plants during Terrestrial Succession", "uid": "p0010315", "west": -59.666116}, {"awards": "1643534 Cassar, Nicolas", "bounds_geometry": "POLYGON((-83 -62,-80.3 -62,-77.6 -62,-74.9 -62,-72.2 -62,-69.5 -62,-66.8 -62,-64.1 -62,-61.4 -62,-58.7 -62,-56 -62,-56 -63.1,-56 -64.2,-56 -65.3,-56 -66.4,-56 -67.5,-56 -68.6,-56 -69.7,-56 -70.8,-56 -71.9,-56 -73,-58.7 -73,-61.4 -73,-64.1 -73,-66.8 -73,-69.5 -73,-72.2 -73,-74.9 -73,-77.6 -73,-80.3 -73,-83 -73,-83 -71.9,-83 -70.8,-83 -69.7,-83 -68.6,-83 -67.5,-83 -66.4,-83 -65.3,-83 -64.2,-83 -63.1,-83 -62))", "dataset_titles": "Palmer LTER 18S rRNA gene metabarcodin; rDNA amplicon sequencing of WAP microbial community", "datasets": [{"dataset_uid": "200286", "doi": "", "keywords": null, "people": null, "repository": "NCBI", "science_program": null, "title": "rDNA amplicon sequencing of WAP microbial community", "url": "https://www.ncbi.nlm.nih.gov/sra/SRR6162326/"}, {"dataset_uid": "200285", "doi": "", "keywords": null, "people": null, "repository": "NCBI", "science_program": null, "title": "Palmer LTER 18S rRNA gene metabarcodin", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA508517"}], "date_created": "Thu, 03 Mar 2022 00:00:00 GMT", "description": "This project seeks to make detailed measurements of the oxygen content of the surface ocean along the Western Antarctic Peninsula. Detailed maps of changes in net oxygen content will be combined with measurements of the surface water chemistry and phytoplankton distributions. The project will determine the extent to which on-shore or offshore phytoplankton blooms along the peninsula are likely to lead to different amounts of carbon being exported to the deeper ocean. The project team members will participate in the development of new learning tools at the Museum of Life and Science. They will also teach secondary school students about aquatic biogeochemistry and climate, drawing directly from the active science supported by this grant. The project will analyze oxygen in relation to argon that will allow determination of the physical and biological contributions to surface ocean oxygen dynamics. These assessments will be combined with spatial and temporal distributions of nutrients (iron and macronutrients) and irradiances. This will allow the investigators to unravel the complex interplay between ice dynamics, iron and physical mixing dynamics as they relate to Net Community Production (NCP) in the region. NCP measurements will be normalized to Particulate Organic Carbon (POC) and be used to help identify area of \"High Biomass and Low NCP\" and those with \"Low Biomass and High NCP\" as a function of microbial plankton community composition. The team will use machine learning methods- including decision tree assemblages and genetic programming- to identify plankton groups key to facilitating biological carbon fluxes. Decomposing the oxygen signal along the West Antarctic Peninsula will also help elucidate biotic and abiotic drivers of the O2 saturation to further contextualize the growing inventory of oxygen measurements (e.g. by Argo floats) throughout the global oceans.", "east": -56.0, "geometry": "POINT(-69.5 -67.5)", "instruments": null, "is_usap_dc": true, "keywords": "West Antarctica; USAP-DC; BIOGEOCHEMICAL CYCLES; AMD; USA/NSF; LABORATORY; Amd/Us", "locations": "West Antarctica", "north": -62.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Cassar, Nicolas", "platforms": "OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repo": "NCBI", "repositories": "NCBI", "science_programs": null, "south": -73.0, "title": "Biological and Physical Drivers of Oxygen Saturation and Net Community Production Variability along the Western Antarctic Peninsula", "uid": "p0010303", "west": -83.0}, {"awards": "1643917 Fricker, Helen", "bounds_geometry": "POLYGON((-163.646 -84.186,-162.58715 -84.186,-161.5283 -84.186,-160.46945 -84.186,-159.4106 -84.186,-158.35175 -84.186,-157.2929 -84.186,-156.23405 -84.186,-155.1752 -84.186,-154.11635 -84.186,-153.0575 -84.186,-153.0575 -84.20871,-153.0575 -84.23142,-153.0575 -84.25413,-153.0575 -84.27684,-153.0575 -84.29955,-153.0575 -84.32226,-153.0575 -84.34497,-153.0575 -84.36768,-153.0575 -84.39039,-153.0575 -84.4131,-154.11635 -84.4131,-155.1752 -84.4131,-156.23405 -84.4131,-157.2929 -84.4131,-158.35175 -84.4131,-159.4106 -84.4131,-160.46945 -84.4131,-161.5283 -84.4131,-162.58715 -84.4131,-163.646 -84.4131,-163.646 -84.39039,-163.646 -84.36768,-163.646 -84.34497,-163.646 -84.32226,-163.646 -84.29955,-163.646 -84.27684,-163.646 -84.25413,-163.646 -84.23142,-163.646 -84.20871,-163.646 -84.186))", "dataset_titles": "Wideband magnetotelluric responses from Whillans Ice Stream, West Antarctica", "datasets": [{"dataset_uid": "601526", "doi": "10.15784/601526", "keywords": "Antarctica; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Ice Stream; Magnetotelluric; Subglacial; Whillans Ice Stream", "people": "Siegfried, Matthew; Key, Kerry; Gustafson, Chloe; Fricker, Helen", "repository": "USAP-DC", "science_program": null, "title": "Wideband magnetotelluric responses from Whillans Ice Stream, West Antarctica", "url": "https://www.usap-dc.org/view/dataset/601526"}], "date_created": "Sat, 26 Feb 2022 00:00:00 GMT", "description": "The Antarctic ice sheet is underlain by a dynamic water system that lubricates the flow of ice streams and outlet glaciers, provides a habitat for a diverse microbial ecosystem, and delivers freshwater and nutrients to the Southern Ocean. However, imaging this subglacial environment is difficult: Antarctica is a vast continent with ice up to four kilometers (2.5 miles) thick. To detect water at the ice-bed interface and in deeper groundwater reservoirs, this project will adapt a technique called electromagnetic sounding that is well-established on land and in the ocean for imaging fluids beneath the surface. Groundwater is estimated to be a significant part of the subglacial water budget in Antarctica, yet previous observational approaches have been unable to characterize its volume and distribution. This project will thus yield critical information about how ice-rock-water-ocean systems interact and inform our understanding of ice-sheet processes, global nutrient cycles, and freshwater flux to the ocean. The project will provide cross-disciplinary training for a graduate student and postdoctoral scientist, and develop an educational outreach program through the Birch Aquarium. Standard geophysical surveying techniques used in glaciology to image subglacial water (radio-echo sounding and active-source seismology) are not directly sensitive to water content. In contrast, ground-based electromagnetic (EM) methods are sensitive to water content through its impact on bulk conductivity. Although EM methods are well-established for high-precision mapping of hydrology in other geological environments, their application on ice sheets is in its infancy. The proposed work will adapt both passive- and active-source EM techniques to glaciological questions to quantify the three-dimensional structure of subglacial water beneath an ice stream and in a grounding zone. The project will perform a suite of synthetic inversion studies to determine the range of applications of EM techniques in glaciology and execute a field experiment on the Whillans Ice Plain to investigate two hypotheses about the subglacial water system based on previous observational and modeling results: (1) Subglacial Lake Whillans is underlain by a deep, saline groundwater reservoir; and (2) there is an estuary-like zone of mixing between fresh subglacial water and seawater near, and possibly landward, of the grounding line.", "east": -153.0575, "geometry": "POINT(-158.35175 -84.29955)", "instruments": null, "is_usap_dc": true, "keywords": "Whillans Ice Stream; GROUND WATER; USA/NSF; USAP-DC; AMD; GEOMAGNETIC INDUCTION; Amd/Us; FIELD SURVEYS", "locations": "Whillans Ice Stream", "north": -84.186, "nsf_funding_programs": "Antarctic Glaciology", "paleo_time": null, "persons": "Key, Kerry; Fricker, Helen; Siegfried, Matthew", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -84.4131, "title": "Mapping Antarctic Subglacial Water with Novel Electromagnetic Techniques", "uid": "p0010300", "west": -163.646}, {"awards": "1847067 Levy, Joseph", "bounds_geometry": "POLYGON((161 -76,161.35 -76,161.7 -76,162.05 -76,162.4 -76,162.75 -76,163.1 -76,163.45 -76,163.8 -76,164.15 -76,164.5 -76,164.5 -76.2,164.5 -76.4,164.5 -76.6,164.5 -76.8,164.5 -77,164.5 -77.2,164.5 -77.4,164.5 -77.6,164.5 -77.8,164.5 -78,164.15 -78,163.8 -78,163.45 -78,163.1 -78,162.75 -78,162.4 -78,162.05 -78,161.7 -78,161.35 -78,161 -78,161 -77.8,161 -77.6,161 -77.4,161 -77.2,161 -77,161 -76.8,161 -76.6,161 -76.4,161 -76.2,161 -76))", "dataset_titles": "Biogeochemical measurements of water tracks and adjacent dry soils from the McMurdo Dry Valleys; Surface Water Geochemistry from the McMurdo Dry Valleys", "datasets": [{"dataset_uid": "601684", "doi": "10.15784/601684", "keywords": "Antarctica; Cation Exchange; Chemistry:soil; Chemistry:Soil; Dry Valleys; Organic Matter; Salt; Soil", "people": "Levy, Joseph", "repository": "USAP-DC", "science_program": null, "title": "Biogeochemical measurements of water tracks and adjacent dry soils from the McMurdo Dry Valleys", "url": "https://www.usap-dc.org/view/dataset/601684"}, {"dataset_uid": "601703", "doi": "10.15784/601703", "keywords": "Antarctica; Dry Valleys", "people": "Levy, Joseph", "repository": "USAP-DC", "science_program": null, "title": "Surface Water Geochemistry from the McMurdo Dry Valleys", "url": "https://www.usap-dc.org/view/dataset/601703"}], "date_created": "Fri, 24 Dec 2021 00:00:00 GMT", "description": "Antarctic groundwater drives the regional carbon cycle and can accelerate permafrost thaw shaping Antarctic surface features. However, groundwater extent, flow, and processes on a continent virtually locked in ice are poorly understood. The proposed work investigates the interplay between groundwater, sediment, and ice in Antarctica\u0027s cold desert landscape to determine when, where, and why Antarctic groundwater is flowing, and how it may evolve Antarctic frozen deserts from dry and stable to wet and dynamic. Mapping the changing extent of Antarctic near-surface groundwater requires the ability to measure soil moisture rapidly and repeatedly over large areas. The research will capture changes in near-surface groundwater distribution through an unmanned aerial vehicle (UAV) mapping approach. The project integrates a diverse range of sensors with new UAV technologies to provide a higher-resolution and more frequent assessment of Antarctic groundwater extent and composition than can be accomplished using satellite observations alone. To complement the research objectives, the PI will develop a new UAV summer field school, the Geosciences UAV Academy, focused on training undergraduate-level UAV pilots in conducting novel earth sciences research using cutting edge imaging tools. The integration of research and technology will prepare students for careers in UAV-related industries and research. The project will deliver new UAV tools and workflows for soil moisture mapping relevant to arid regions including Antarctica as well as temperate desert and dryland systems and will train student research pilots to tackle next generation airborne challenges. Water tracks are the basic hydrological unit that currently feeds the rapidly-changing permafrost and wetlands in the Antarctic McMurdo Dry Valleys (MDV). Despite the importance of water tracks in the MDV hydrologic cycle and their influence on biogeochemistry, little is known about how these water tracks control the unique brine processes operating in Antarctic ice-free areas. Both groundwater availability and geochemistry shape Antarctic microbial communities, connecting soil geology and hydrology to carbon cycling and ecosystem functioning. The objectives of this CAREER proposal are to 1) map water tracks to determine the spatial distribution and seasonal magnitude of groundwater impacts on the MDV near-surface environment to determine how near-surface groundwater drives permafrost thaw and enhances chemical weathering and biogeochemical cycling; 2) establish a UAV academy training earth sciences students to answer geoscience questions using drone-based platforms and remote sensing techniques; and 3) provide a formative step in the development of the PI as a teacher-scholar. UAV-borne hyperspectral imaging complemented with field soil sampling will determine the aerial extent and timing of inundation, water level, and water budget of representative water tracks in the MDV. Soil moisture will be measured via near-infrared reflectance spectroscopy while bulk chemistry of soils and groundwater will be analyzed via ion chromatography and soil x-ray fluorescence. Sedimentological and hydrological properties will be determined via analysis of intact core samples. These data will be used to test competing hypotheses regarding the origin of water track solutions and water movement through seasonal wetlands. The work will provide a regional understanding of groundwater sources, shallow groundwater flux, and the influence of regional hydrogeology on solute export to the Southern Ocean and on soil/atmosphere linkages in earth\u0027s carbon budget. The UAV school will 1) provide comprehensive instruction at the undergraduate level in both how and why UAVs can advance geoscience research and learning; and 2) provide educational infrastructure for an eventual self-sustaining summer program for undergraduate UAV education. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 164.5, "geometry": "POINT(162.75 -77)", "instruments": null, "is_usap_dc": true, "keywords": "FIELD SURVEYS; Amd/Us; USA/NSF; AMD; USAP-DC; FROZEN GROUND; Taylor Valley", "locations": "Taylor Valley", "north": -76.0, "nsf_funding_programs": "Antarctic Earth Sciences", "paleo_time": null, "persons": "Levy, Joseph", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -78.0, "title": "Linking Antarctic Cold Desert Groundwater to Thermokarst \u0026 Chemical Weathering in Partnership with the Geoscience UAV Academy", "uid": "p0010286", "west": 161.0}, {"awards": "1744785 Barrett, John", "bounds_geometry": "POLYGON((-180 -77.62,-145.683 -77.62,-111.366 -77.62,-77.049 -77.62,-42.732 -77.62,-8.415 -77.62,25.902 -77.62,60.219 -77.62,94.536 -77.62,128.853 -77.62,163.17 -77.62,163.17 -77.618,163.17 -77.616,163.17 -77.614,163.17 -77.612,163.17 -77.61,163.17 -77.608,163.17 -77.606,163.17 -77.604,163.17 -77.602,163.17 -77.6,128.853 -77.6,94.536 -77.6,60.219 -77.6,25.902 -77.6,-8.415 -77.6,-42.732 -77.6,-77.049 -77.6,-111.366 -77.6,-145.683 -77.6,180 -77.6,178.319 -77.6,176.638 -77.6,174.957 -77.6,173.276 -77.6,171.595 -77.6,169.914 -77.6,168.233 -77.6,166.552 -77.6,164.871 -77.6,163.19 -77.6,163.19 -77.602,163.19 -77.604,163.19 -77.606,163.19 -77.608,163.19 -77.61,163.19 -77.612,163.19 -77.614,163.19 -77.616,163.19 -77.618,163.19 -77.62,164.871 -77.62,166.552 -77.62,168.233 -77.62,169.914 -77.62,171.595 -77.62,173.276 -77.62,174.957 -77.62,176.638 -77.62,178.319 -77.62,-180 -77.62))", "dataset_titles": "McMurdo Dry Valleys LTER: Microbial mat biomass and Normalized Difference Vegetation Index (NDVI) values from Lake Fryxell Basin, Antarctica", "datasets": [{"dataset_uid": "200260", "doi": "doi:10.6073/pasta/9acbbde9abc1e013f8c9fd9c383327f4", "keywords": null, "people": null, "repository": "EDI", "science_program": null, "title": "McMurdo Dry Valleys LTER: Microbial mat biomass and Normalized Difference Vegetation Index (NDVI) values from Lake Fryxell Basin, Antarctica", "url": "https://doi.org/10.6073/pasta/9acbbde9abc1e013f8c9fd9c383327f4"}], "date_created": "Tue, 30 Nov 2021 00:00:00 GMT", "description": "Microbial mats are found throughout the McMurdo Dry Valleys where summer snowmelt provides liquid water that allows these mats to flourish. Researchers have long studied the environmental conditions microbial mats need to grow. Despite these efforts, it has been difficult to develop a broad picture of these unique ecosystems. Recent advances in satellite technology now provide researchers an exciting new tool to study these special Antarctic ecosystems from space using the unique spectral signatures associated with microbial mats. This new technology not only offers the promise that microbial mats can be mapped and studied from space, this research will also help protect these delicate environments from potentially harmful human impacts that can occur when studying them from the ground. This project will use satellite imagery and spectroscopic techniques to identify and map microbial mat communities and relate their properties and distributions to both field and lab-based measurements. This research provides an exciting new tool to help document and understand the distribution of a major component of the Antarctic ecosystem in the McMurdo Dry Valleys. The goal of this project is to establish quantitative relationships between spectral signatures derived from orbit and the physiological status and biogeochemical properties of microbial mat communities in Taylor Valley, Antarctica, as measured by field and laboratory analyses on collected samples. The goal wioll be met by (1) refining atmospheric correction techniques using in situ radiometric rectification to derive accurate surface spectra; (2) collecting multispectral orbital images concurrent with in situ sampling and spectral measurements in the field to ensure temporal comparability; (3) measuring sediment, water, and microbial mat samples for organic and inorganic carbon content, essential biogeochemical nutrients, and chlorophyll-a to determine relevant mat characteristics; and (4) quantitatively associating these laboratory-derived characteristics with field-derived and orbital spectral signatures and parameters. The result of this work will be a more robust quantitative link between the distribution of microbial mat communities and their biogeochemical properties to landscape-scale spectral signatures. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 163.19, "geometry": "POINT(-16.82 -77.61)", "instruments": null, "is_usap_dc": true, "keywords": "AMD; ECOSYSTEM FUNCTIONS; FIELD SURVEYS; USAP-DC; USA/NSF; Taylor Valley; Amd/Us", "locations": "Taylor Valley", "north": -77.6, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Barrett, John; Salvatore, Mark", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS", "repo": "EDI", "repositories": "EDI", "science_programs": null, "south": -77.62, "title": "Collaborative Research: Remote characterization of microbial mats in Taylor Valley, Antarctica through in situ sampling and spectral validation", "uid": "p0010281", "west": 163.17}, {"awards": "1947562 van Gestel, Natasja; 1643871 van Gestel, Natasja", "bounds_geometry": "POLYGON((-65 -64.5,-64.8 -64.5,-64.6 -64.5,-64.4 -64.5,-64.2 -64.5,-64 -64.5,-63.8 -64.5,-63.6 -64.5,-63.4 -64.5,-63.2 -64.5,-63 -64.5,-63 -64.55,-63 -64.6,-63 -64.65,-63 -64.7,-63 -64.75,-63 -64.8,-63 -64.85,-63 -64.9,-63 -64.95,-63 -65,-63.2 -65,-63.4 -65,-63.6 -65,-63.8 -65,-64 -65,-64.2 -65,-64.4 -65,-64.6 -65,-64.8 -65,-65 -65,-65 -64.95,-65 -64.9,-65 -64.85,-65 -64.8,-65 -64.75,-65 -64.7,-65 -64.65,-65 -64.6,-65 -64.55,-65 -64.5))", "dataset_titles": "2022-2023 Palmer Station terrestrial carbon fluxes - field warming experiment; Soil moisture and soil temperature data (0-5 cm) near Palmer Station, Antarctica", "datasets": [{"dataset_uid": "601853", "doi": "10.15784/601853", "keywords": "Antarctica; CO2; Cryosphere; Field Investigations; Palmer Station", "people": "van Gestel, Natasja", "repository": "USAP-DC", "science_program": null, "title": "2022-2023 Palmer Station terrestrial carbon fluxes - field warming experiment", "url": "https://www.usap-dc.org/view/dataset/601853"}, {"dataset_uid": "601877", "doi": "10.15784/601877", "keywords": "Antarctica; Antarctic Peninsula; Conductivity; Cryosphere; Palmer Station; Soil; Temperature", "people": "van Gestel, Natasja", "repository": "USAP-DC", "science_program": null, "title": "Soil moisture and soil temperature data (0-5 cm) near Palmer Station, Antarctica", "url": "https://www.usap-dc.org/view/dataset/601877"}], "date_created": "Sat, 21 Aug 2021 00:00:00 GMT", "description": "Part I: Non-technical description: Earth\u2019s terrestrial ecosystems have the potential to either slow down or hasten the pace of climate change. The direction depends in part on both plant and microbial responses to warming. This study uses Antarctica as a model ecosystem to study the carbon balance of a simplified ecosystem (simplified compared to terrestrial ecosystems elsewhere) in response to a warming treatment. Carbon balance is dictated by sequestered carbon (through photosynthesis) and released carbon (plant and microbial respiration). Hence, to best assess plant and microbial responses to warming, this study uses a plant gradient that starts at the glacier (no plants, only soil microbes) to an old site entirely covered by plants. Experimental warming in the field is achieved by open-top chambers that warm the air and soil inside. The net ecosystem carbon exchange, the net result of sequestered and released carbon, will be measured in warmed and control plots with a state-of-the art gas exchange machine. Laboratory temperature incubation studies will supplement field work to attribute changes in carbon fluxes to individual plant species and soil microbial taxa (i.e., \u201cspecies\u201d). Data from this study will feed into earth system climate change models. The importance of this study will be shared with the broader community through the production of a video series created by an award-winning science media production company, an Antarctic blog, and through interactions with schools in the United States (on-site through Skype and in-person visits). Part II: Technical description: Responses of the carbon balance of terrestrial ecosystems to warming will feed back to the pace of climate change, but the size and direction of this feedback are poorly constrained. Least known are the effects of warming on carbon losses from soil, and clarifying the major microbial controls is an important research frontier. This study uses a series of experiments and observations to investigate microbial, including autotrophic taxa, and plant controls of net ecosystem productivity in response to warming in intact ecosystems. Field warming is achieved using open-top chambers paired with control plots, arrayed along a productivity gradient. Along this gradient, incoming and outgoing carbon fluxes will be measured at the ecosystem-level. The goal is to tie warming-induced shifts in net ecosystem carbon balance to warming effects on soil microbes and plants. The field study will be supplemented with lab temperature incubations. Because soil microbes dominate biogeochemical cycles in Antarctica, a major focus of this study is to determine warming responses of bacteria, fungi and archaea. This is achieved using a cutting-edge stable isotope technique, quantitative stable isotope probing (qSIP) developed by the proposing research team, that can identify the taxa that are active and involved in processing new carbon. This technique can identify individual microbial taxa that are actively participating in biogeochemical cycling of nutrients (through combined use of 18O-water and 13C-bicarbonate) and thus can be distinguished from those that are simply present (cold-preserved). The study further assesses photosynthetic uptake of carbon by the vegetation and their sensitivity to warming. Results will advance research in climate change, plant and soil microbial ecology, and ecosystem modeling. Science communication will be achieved through an informative video series, a daily Antarctic blog, and online- and in-person visits to schools in the United States. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": -63.0, "geometry": "POINT(-64 -64.75)", "instruments": null, "is_usap_dc": true, "keywords": "Palmer Station; TERRESTRIAL ECOSYSTEMS; USA/NSF; AMD; Amd/Us; USAP-DC; FIELD SURVEYS", "locations": "Palmer Station", "north": -64.5, "nsf_funding_programs": "Antarctic Integrated System Science; Antarctic Organisms and Ecosystems; Antarctic Earth Sciences", "paleo_time": null, "persons": "van Gestel, Natasja", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -65.0, "title": "Antarctica as a Model System for Responses of Terrestrial Carbon Balance to Warming", "uid": "p0010251", "west": -65.0}, {"awards": "2046800 Thurber, Andrew", "bounds_geometry": "POLYGON((162 -77,162.6 -77,163.2 -77,163.8 -77,164.4 -77,165 -77,165.6 -77,166.2 -77,166.8 -77,167.4 -77,168 -77,168 -77.1,168 -77.2,168 -77.3,168 -77.4,168 -77.5,168 -77.6,168 -77.7,168 -77.8,168 -77.9,168 -78,167.4 -78,166.8 -78,166.2 -78,165.6 -78,165 -78,164.4 -78,163.8 -78,163.2 -78,162.6 -78,162 -78,162 -77.9,162 -77.8,162 -77.7,162 -77.6,162 -77.5,162 -77.4,162 -77.3,162 -77.2,162 -77.1,162 -77))", "dataset_titles": null, "datasets": null, "date_created": "Sat, 21 Aug 2021 00:00:00 GMT", "description": "Part I: Non-technical description: Methane is one of the more effective atmospheric gases at retaining heat in the lower atmosphere and the earth\u2019s crust contains large quantities of methane. Research that identifies the factors that control methane\u2019s release into the atmosphere is critical to understanding and mitigating climate change. One of the most effective natural processes that inhibits the release of methane from aquatic habitats is a community of bacteria and Archaea (microbes) that use the chemical energy stored in methane, transforming methane into less-climate-sensitive compounds. The amount of methane that may be released in Antarctica is unknown, and it is unclear which microbes consume the methane before it is released from the ocean in Antarctica. This project will study one of the few methane seeps known in Antarctica to advance our understanding of which microbes inhibit the release of methane in marine environments. The research will also identify if methane is a source of energy for other Antarctic organisms. The researchers will analyze the microbial species associated with methane consumption over several years of field and laboratory research based at an Antarctic US station, McMurdo. This project clearly expands the fundamental knowledge of Antarctic systems, biota, and processes outlined as a goal in the Antarctic solicitation. This research communicates and produces educational material for K-12, college, and graduate students to inspire and inform the public about the role Antarctic ecosystems play in the global environment. This project also provides a young professor an opportunity to establish himself as an expert in the field of Antarctic microbial ecology to help solidify his academic career. Part II: Technical description: Microbes act as filter to methane release from the ocean into the atmosphere, where microbial chemosynthetic production harvests the chemical energy stored in this greenhouse gas. In spite of methane reservoirs in Antarctica being as large as Arctic permafrost, we know only a little about the taxa or dominant processes involved in methane consumption in Antarctica. The principal investigator will undertake a genomic and transcriptomic study of microbial communities developed and still developing after initiation of methane seepage in McMurdo Sound. An Antarctic methane seep was discovered at this location in 2012 after it began seeping in 2011. Five years after it began releasing methane, the methane-oxidizing microbial community was underdeveloped and methane was still escaping from the seafloor. This project will be essential in elucidating the response of microbial communities to methane release and identify how methane oxidation occurs within the constraints of the low polar temperatures. This investigation is based on 4 years of field sampling and will establish a time series of the development of cold seep microbial communities in Antarctica. A genome-to-ecosystem approach will establish how the Southern Ocean microbial community is adapted to prevent methane release into the ocean. As methane is an organic carbon source, results from this study will have implications for the Southern Ocean carbon cycle. Two graduate students will be trained and supported with undergraduates participating in laboratory activities. The researcher aims to educate, inspire and communicate about Antarctic methane seeps to a broad community. A mixed-media approach, with videos, art and education in schools will be supported in collaboration with a filmmaker, teachers and a visual artist. Students will be trained in filmmaking and K-12 students from under-represented communities will be introduced to Antarctic science through visual arts. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 168.0, "geometry": "POINT(165 -77.5)", "instruments": null, "is_usap_dc": true, "keywords": "AMD; USA/NSF; USAP-DC; BACTERIA/ARCHAEA; McMurdo Sound; BENTHIC; FIELD SURVEYS; Amd/Us; ECOSYSTEM FUNCTIONS", "locations": "McMurdo Sound", "north": -77.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Thurber, Andrew", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS", "repositories": null, "science_programs": null, "south": -78.0, "title": "CAREER: Ecosystem Impacts of Microbial Succession and Production at Antarctic Methane Seeps", "uid": "p0010250", "west": 162.0}, {"awards": "1644155 Twining, Benjamin", "bounds_geometry": "POLYGON((78 -68.4,78.05 -68.4,78.1 -68.4,78.15 -68.4,78.2 -68.4,78.25 -68.4,78.3 -68.4,78.35 -68.4,78.4 -68.4,78.45 -68.4,78.5 -68.4,78.5 -68.419,78.5 -68.438,78.5 -68.457,78.5 -68.476,78.5 -68.495,78.5 -68.514,78.5 -68.533,78.5 -68.552,78.5 -68.571,78.5 -68.59,78.45 -68.59,78.4 -68.59,78.35 -68.59,78.3 -68.59,78.25 -68.59,78.2 -68.59,78.15 -68.59,78.1 -68.59,78.05 -68.59,78 -68.59,78 -68.571,78 -68.552,78 -68.533,78 -68.514,78 -68.495,78 -68.476,78 -68.457,78 -68.438,78 -68.419,78 -68.4))", "dataset_titles": "Flow cytometry enumeration of virus-like and bacteria-like abundance in Ace, Deep, \u0026 Organic lakes (Antarctica)", "datasets": [{"dataset_uid": "601626", "doi": "10.15784/601626", "keywords": "Ace Lake; Antarctica; Deep Lake; Organic Lake; Vestfold Hills", "people": "Twining, Benjamin; Martinez-Martinez, Joaquin", "repository": "USAP-DC", "science_program": null, "title": "Flow cytometry enumeration of virus-like and bacteria-like abundance in Ace, Deep, \u0026 Organic lakes (Antarctica)", "url": "https://www.usap-dc.org/view/dataset/601626"}], "date_created": "Fri, 06 Aug 2021 00:00:00 GMT", "description": "Viruses are prevalent in aquatic environments where they reach up to five hundred million virus particles in a teaspoon of water. Ongoing discovery of viruses seems to confirm current understanding that all forms of life can host and be infected by viruses and that viruses are one of the largest reservoirs of unexplored genetic diversity on Earth. This study aims to better understand interactions between specific viruses and phytoplankton hosts and determine how these viruses may affect different algal groups present within lakes of the Vestfold Hills, Antarctica. These lakes (Ace, Organic and Deep)were originally derived from the ocean and contain a broad range of saline conditions with a similarly broad range of physicochemical characteristics resulting from isolation and low external influence for thousands of years. These natural laboratories allow examination of microbial processes and interactions that would be difficult to characterize elsewhere on earth. The project will generate extensive genomic information that will be made freely available. The project will also leverage the study of viruses and the genomic approaches employed to advance the training of undergraduate students and to engage and foster an understanding of Antarctic science and studies of microbes during a structured informal education program in Maine for the benefit of high school students. By establishing the dynamics and interactions of (primarily) specific dsDNA virus groups in different habitats with different redox conditions throughout seasonal and inter annual cycles the project will learn about the biotic and abiotic factors that influence microbial community dynamics. This project does not require fieldwork in Antarctica. Instead, the investigators will leverage already collected and archived samples from three lakes that have concurrent measures of physicochemical information. Approximately 2 terabyte of Next Generation Sequencing (NGS) (including metagenomes, SSU rRNA amplicons and single virus genomes) will be generated from selected available samples through a Community Science Program (CSP) funded by the Joint Genome Institute. The investigators will employ bioinformatics to interrogate those sequence databases. In particular, they will focus on investigating the presence, phylogeny and co-occurrence of polintons, polinton-like viruses, virophages and large dsDNA phytoplankton viruses as well as of their putative eukaryotic microbial hosts. Bioinformatic analyses will be complemented with quantitative digital PCR and microbial association network analysis to detect specific virus?virus?host interactions from co-occurrence spatial and temporal patterns. Multivariate analysis and network analyses will also be performed to investigate which abiotic factors most closely correlate with phytoplankton and virus abundances, temporal dynamics, and observed virus-phytoplankton associations within the three lakes. The results of this project will improve understanding of phytoplankton and their viruses as vital components of the carbon cycle in Antarctic, marine-derived aquatic environments, and likely in any other aquatic environment. Overall, this work will advance understanding of the genetic underpinnings of adaptations in unique Antarctic environments.", "east": 78.5, "geometry": "POINT(78.25 -68.495)", "instruments": null, "is_usap_dc": true, "keywords": "LABORATORY; AMD; USAP-DC; VIRUSES; Vestfold Hills; Amd/Us; FIELD SURVEYS; USA/NSF", "locations": "Vestfold Hills", "north": -68.4, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Twining, Benjamin; Martinez-Martinez, Joaquin", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS; OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -68.59, "title": "Viral control of microbial communities in Antarctic lakes", "uid": "p0010237", "west": 78.0}, {"awards": "2031442 Learman, Deric", "bounds_geometry": "POLYGON((-180 -60,-167.5 -60,-155 -60,-142.5 -60,-130 -60,-117.5 -60,-105 -60,-92.5 -60,-80 -60,-67.5 -60,-55 -60,-55 -62,-55 -64,-55 -66,-55 -68,-55 -70,-55 -72,-55 -74,-55 -76,-55 -78,-55 -80,-67.5 -80,-80 -80,-92.5 -80,-105 -80,-117.5 -80,-130 -80,-142.5 -80,-155 -80,-167.5 -80,180 -80,178 -80,176 -80,174 -80,172 -80,170 -80,168 -80,166 -80,164 -80,162 -80,160 -80,160 -78,160 -76,160 -74,160 -72,160 -70,160 -68,160 -66,160 -64,160 -62,160 -60,162 -60,164 -60,166 -60,168 -60,170 -60,172 -60,174 -60,176 -60,178 -60,-180 -60))", "dataset_titles": "Physical and geochemical data from shelf sediments near the Antartic Pennisula", "datasets": [{"dataset_uid": "601607", "doi": "10.15784/601607", "keywords": "Antarctica; Antarctic Peninsula; Grain Size; Grain Size Analysis; Marine Geoscience; Marine Sediments; Organic Matter Geochemistry; Sediment Core Data; Shelf Sediments; Weddell Sea", "people": "Learman, Deric", "repository": "USAP-DC", "science_program": null, "title": "Physical and geochemical data from shelf sediments near the Antartic Pennisula", "url": "https://www.usap-dc.org/view/dataset/601607"}], "date_created": "Wed, 28 Jul 2021 00:00:00 GMT", "description": "Western Antarctica is one of the fastest warming locations on Earth. Its changing climate will lead to an increase in sea-level and will also alter regional water temperature and chemistry. These changes will directly alter the microbes that inhabit the ecosystem. Microbes are the smallest forms of life on Earth, but they are also the most abundant. They drive cycling of essential nutrients, such as carbon and nitrogen that are found in ocean sediments. In this way they form the foundation of the food chain that supports larger and more complex life. However, we do not know much about how different communities of microbes break down sediments in Antarctica and this will influence the chemistry of those waters. This research will determine how communities of microbes on the coastal shelf of Antarctica degrade complex organic sediments using genetic and chemical data. This data will identify the species in the community, what enzymes they are producing and what chemical reactions they are driving. This research will create broader impacts as the data will be used to create in-class activities that improve a student\u2019s data analysis and critical thinking skills. The data will be used in graduate, undergraduate and K-12 classrooms. This research will provide genetic and enzymatic insight into how microbial communities in benthic sediments on the coastal shelf of Antarctica degrade complex organic matter. The current understanding of how benthic microbial communities respond to and then degrade complex organic matter in Antarctica is fragmented. Recent work suggests benthic microbial communities are shaped by organic matter availability. However, those studies were observational and did not directly examine community function. A preliminary study of metagenomic data from western Antarctic marine sediments, indicates a genetic potential for organic matter degradation but functional data was not been collected. Other studies have examined either enzyme activity or metagenomic potential, but few have been able to directly connect the two. To address this gap in knowledge, this study will utilize metagenomics and metatranscriptomics, coupled with microcosm experiments, enzyme assays, and geochemical data. It will examine Antarctic microbial communities from the Ross Sea, the Bransfield Strait and Weddell Sea to document how the relationship between a communities\u2019 enzymatic activity and the genes used to degrade complex organic matter is related to sediment breakdown. The data will expand our current knowledge of microbial genetic potential and provide a solid understanding of enzyme function as it relates to degradation of complex organic matter in those marine sediments. It will thereby improve our understanding of temperature change on the chemistry of Antarctic seawater. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 160.0, "geometry": "POINT(-127.5 -70)", "instruments": null, "is_usap_dc": true, "keywords": "ECOSYSTEM FUNCTIONS; USAP-DC; Antarctic Peninsula; BENTHIC; SHIPS; SEDIMENT CHEMISTRY; Amd/Us; AMD; USA/NSF; Weddell Sea", "locations": "Antarctic Peninsula; Weddell Sea", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Learman, Deric", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e SHIPS", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -80.0, "title": "RAPID: Meta-genomic and Transcriptomic Investigation of Complex Organic Matter Degradation in Antarctic Benthic Sediments", "uid": "p0010235", "west": -55.0}, {"awards": "1947040 Postlethwait, John", "bounds_geometry": "POLYGON((-65.3 -63.3,-65 -63.3,-64.7 -63.3,-64.4 -63.3,-64.1 -63.3,-63.8 -63.3,-63.5 -63.3,-63.2 -63.3,-62.9 -63.3,-62.6 -63.3,-62.3 -63.3,-62.3 -63.47,-62.3 -63.64,-62.3 -63.81,-62.3 -63.98,-62.3 -64.15,-62.3 -64.32,-62.3 -64.49,-62.3 -64.66,-62.3 -64.83,-62.3 -65,-62.6 -65,-62.9 -65,-63.2 -65,-63.5 -65,-63.8 -65,-64.1 -65,-64.4 -65,-64.7 -65,-65 -65,-65.3 -65,-65.3 -64.83,-65.3 -64.66,-65.3 -64.49,-65.3 -64.32,-65.3 -64.15,-65.3 -63.98,-65.3 -63.81,-65.3 -63.64,-65.3 -63.47,-65.3 -63.3))", "dataset_titles": "18 SSU rDNA type sequences for Notoxcellia coronata (nov. sp.); 18 SSU rDNA type sequences for Notoxcellia picta (nov. sp.); Expedition Data of LMG1805; Fish pictures and skin pathology of X-cell infection in Trematomus scotti.; Gonad and skin histology of Trematomus loennbergii infected by Notoxcellia sp.; Histopathology of X-cell xenomas in Trematomus scotti and Nototheniops larseni.; In situ hybridization of X-cell and host fish 18S SSU rRNA in alternate sections of tumor xenomas.; Metagenomic analysis of apparently healthy and tumor samples using Kaiju software ; microMRI analyses of Trematomus scotti Tsco_18_08 with X-cell xenomas; Morphological and pathological data of Trematomus scotti specimens captured on May 30th, 2018 in Andvord Bay.; Morphological, fecundity, and age data of Trematomus scotti from Andvord Bay and the Weddell Sea.; Nomenclatural Act for the genus Notoxcellia; Nomenclatural Act for the species Notoxcellia coronata; Nomenclatural Act for the species Notoxcellia picta; Phylogenetic Analysis of Notoxcellia species.; Phylogenetic Analysis of Notoxcellia species, including novel Ross Sea specimen; Raw Illumina sequencing reads from skin tumors and visually healthy skins from Trematomus scotti and Nototheniops larseni; Similarity matrices of Notoxcellia spp.; Temperature profiles at five fishing locations on the West Antarctic Peninsula during austral fall 2018.; Trematomus scotti mt-co1 sequence alignment.; Trematomus scotti with X-cell xenomas", "datasets": [{"dataset_uid": "601917", "doi": "10.15784/601917", "keywords": "Alveolata; Antarctic; Cryosphere; Notoxcellia Coronata; Notoxcellia Picta; Perkinsozoa; Xcellidae", "people": "Devine, Jennifer; Desvignes, Thomas; Postlethwait, John; P\u00e9ron, Clara", "repository": "USAP-DC", "science_program": null, "title": "Similarity matrices of Notoxcellia spp.", "url": "https://www.usap-dc.org/view/dataset/601917"}, {"dataset_uid": "200254", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data of LMG1805", "url": "https://www.rvdata.us/search/cruise/LMG1805"}, {"dataset_uid": "200443", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data of LMG1805", "url": "https://doi.org/10.7284/907930"}, {"dataset_uid": "601892", "doi": "10.15784/601892", "keywords": "Antarctica; Biota; CO1; COX1; Cryonotothenioid; Cryosphere; Genetic Sequences; LMG1805; MT-CO1; Nototheniidae; Notothenioid; Population Genetics", "people": "Schiavon, Luca ; Postlethwait, John; Desvignes, Thomas; Papetti, Chiara", "repository": "USAP-DC", "science_program": null, "title": "Trematomus scotti mt-co1 sequence alignment.", "url": "https://www.usap-dc.org/view/dataset/601892"}, {"dataset_uid": "601893", "doi": "10.15784/601893", "keywords": "Age; Antarctica; Biota; Cryonotothenioid; Cryosphere; Fecundity; Growth; Length; Nototheniidae; Oceans; Otolith; Reproduction; Weight", "people": "Lucassen, Magnus; Streeter, Margaret; Le Francois, Nathalie; Grondin, Jacob; Valdivieso, Alejandro; Sguotti, Camilla; Postlethwait, John; La Mesa, Mario; Detrich, H. William; Mark, Felix C; Riginella, Emilio; Papetti, Chiara; Desvignes, Thomas; Cal\u00ec, Federico", "repository": "USAP-DC", "science_program": null, "title": "Morphological, fecundity, and age data of Trematomus scotti from Andvord Bay and the Weddell Sea.", "url": "https://www.usap-dc.org/view/dataset/601893"}, {"dataset_uid": "200383", "doi": "", "keywords": null, "people": null, "repository": "ZooBank", "science_program": null, "title": "Nomenclatural Act for the species Notoxcellia coronata", "url": "https://zoobank.org/NomenclaturalActs/194d91b2-e268-4238-89e2-385819f2c35b"}, {"dataset_uid": "601539", "doi": "10.15784/601539", "keywords": "Alveolata; Antarctica; Antarctic Peninsula; Notoxcellia Coronata; Notoxcellia Picta; Perkinsozoa; Xcellidae", "people": "Postlethwait, John; Desvignes, Thomas", "repository": "USAP-DC", "science_program": null, "title": "In situ hybridization of X-cell and host fish 18S SSU rRNA in alternate sections of tumor xenomas.", "url": "https://www.usap-dc.org/view/dataset/601539"}, {"dataset_uid": "601538", "doi": "10.15784/601538", "keywords": "Alveolata; Antarctica; Antarctic Peninsula; Notoxcellia Coronata; Notoxcellia Picta; Perkinsozoa; Xcellidae", "people": "Desvignes, Thomas; Postlethwait, John; Lauridsen, Henrik", "repository": "USAP-DC", "science_program": null, "title": "microMRI analyses of Trematomus scotti Tsco_18_08 with X-cell xenomas", "url": "https://www.usap-dc.org/view/dataset/601538"}, {"dataset_uid": "601537", "doi": "10.15784/601537", "keywords": "Alveolata; Antarctica; Antarctic Peninsula; Notoxcellia Coronata; Notoxcellia Picta; Perkinsozoa; Xcellidae", "people": "Desvignes, Thomas; Postlethwait, John; Varsani, Arvind; Kraberger, Simona ; Fontenele, Rafaela S. ", "repository": "USAP-DC", "science_program": null, "title": "Metagenomic analysis of apparently healthy and tumor samples using Kaiju software ", "url": "https://www.usap-dc.org/view/dataset/601537"}, {"dataset_uid": "601536", "doi": "10.15784/601536", "keywords": "Alveolata; Antarctica; Antarctic Peninsula; Notoxcellia Coronata; Notoxcellia Picta; Perkinsozoa; Xcellidae", "people": "Desvignes, Thomas; Postlethwait, John; Murray, Katrina N. ; Kent, Michael L. ", "repository": "USAP-DC", "science_program": null, "title": "Histopathology of X-cell xenomas in Trematomus scotti and Nototheniops larseni.", "url": "https://www.usap-dc.org/view/dataset/601536"}, {"dataset_uid": "601915", "doi": "10.15784/601915", "keywords": "Alveolata; Antarctic; Cryosphere; Notoxcellia Coronata; Notoxcellia Picta; Perkinsozoa; Xcellidae", "people": "Postlethwait, John; Desvignes, Thomas; P\u00e9ron, Clara; Devine, Jennifer", "repository": "USAP-DC", "science_program": null, "title": "Phylogenetic Analysis of Notoxcellia species, including novel Ross Sea specimen", "url": "https://www.usap-dc.org/view/dataset/601915"}, {"dataset_uid": "601916", "doi": "10.15784/601916", "keywords": "Alveolata; Antarctica; Cryosphere; Notoxcellia Coronata; Notoxcellia Picta; Perkinsozoa; Ross Sea; Xcellidae", "people": "Postlethwait, John; Desvignes, Thomas; P\u00e9ron, Clara; Devine, Jennifer", "repository": "USAP-DC", "science_program": null, "title": "Gonad and skin histology of Trematomus loennbergii infected by Notoxcellia sp.", "url": "https://www.usap-dc.org/view/dataset/601916"}, {"dataset_uid": "601501", "doi": "10.15784/601501", "keywords": "Alveolata; Antarctica; Antarctic Peninsula; Biota; Notoxcellia Coronata; Notoxcellia Picta; Oceans; Perkinsozoa; Xcellidae", "people": "Postlethwait, John; Desvignes, Thomas; Varsani, Arvind", "repository": "USAP-DC", "science_program": null, "title": "Phylogenetic Analysis of Notoxcellia species.", "url": "https://www.usap-dc.org/view/dataset/601501"}, {"dataset_uid": "601496", "doi": "10.15784/601496", "keywords": "Andvord Bay; Antarctica; Fish", "people": "Desvignes, Thomas; Postlethwait, John; Lauridsen, Henrik", "repository": "USAP-DC", "science_program": null, "title": "Fish pictures and skin pathology of X-cell infection in Trematomus scotti.", "url": "https://www.usap-dc.org/view/dataset/601496"}, {"dataset_uid": "601495", "doi": "10.15784/601495", "keywords": "Antarctica; Antarctic Peninsula", "people": "Desvignes, Thomas", "repository": "USAP-DC", "science_program": null, "title": "Temperature profiles at five fishing locations on the West Antarctic Peninsula during austral fall 2018.", "url": "https://www.usap-dc.org/view/dataset/601495"}, {"dataset_uid": "601494", "doi": "10.15784/601494", "keywords": "Andvord Bay; Antarctica; Fish", "people": "Postlethwait, John; Le Francois, Nathalie; Desvignes, Thomas; Lauridsen, Henrik", "repository": "USAP-DC", "science_program": null, "title": "Morphological and pathological data of Trematomus scotti specimens captured on May 30th, 2018 in Andvord Bay.", "url": "https://www.usap-dc.org/view/dataset/601494"}, {"dataset_uid": "200262", "doi": "", "keywords": null, "people": null, "repository": "MorphoSource", "science_program": null, "title": "Trematomus scotti with X-cell xenomas", "url": "https://www.morphosource.org/projects/000405843?locale=en"}, {"dataset_uid": "200275", "doi": "", "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "18 SSU rDNA type sequences for Notoxcellia coronata (nov. sp.)", "url": "https://www.ncbi.nlm.nih.gov/nuccore/OL630144"}, {"dataset_uid": "200276", "doi": "", "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "18 SSU rDNA type sequences for Notoxcellia picta (nov. sp.)", "url": "https://www.ncbi.nlm.nih.gov/nuccore/OL630145"}, {"dataset_uid": "200277", "doi": "", "keywords": null, "people": null, "repository": "NCBI SRA", "science_program": null, "title": "Raw Illumina sequencing reads from skin tumors and visually healthy skins from Trematomus scotti and Nototheniops larseni", "url": "https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA789574"}, {"dataset_uid": "200382", "doi": "", "keywords": null, "people": null, "repository": "ZooBank", "science_program": null, "title": "Nomenclatural Act for the genus Notoxcellia", "url": "https://zoobank.org/NomenclaturalActs/5cf9609e-0111-4386-8518-bd50b5bdde0e"}, {"dataset_uid": "200384", "doi": "", "keywords": null, "people": null, "repository": "ZooBank", "science_program": null, "title": "Nomenclatural Act for the species Notoxcellia picta", "url": "https://zoobank.org/NomenclaturalActs/31062dd2-7202-47fa-86e0-7be5c55ac0e2"}], "date_created": "Thu, 01 Jul 2021 00:00:00 GMT", "description": "Antarctica\u2019s native animals face increasing stressors from warming oceans. A key unanswered question is how Antarctic life will respond. If warmer waters contribute to fish disease susceptibility, then iconic Antarctic predators they support, including penguins, seals, and killer whales, will suffer. A recent scientific cruise on the Antarctic peninsula encountered a population of crowned notothen fish that were plagued by pink, wart-like tumors that covered 10% to 30% of the body surface on about a third of the animals. Similar tumors had not previously been reported, suggesting that this might be a new disease that threatens Antarctic fish. The goal of proposed work is to identify the biological origins of the tumor and how it affects cell function and organismal physiology. The work is potentially transformative because it studies what might be a harbinger of Antarctic fish responses to global climate change. The project has several Broader Impacts. First, it will publicize the tumors. Because Antarctic researchers have never reported a tumor epidemic, the community must become aware of the outbreak and the tumor\u2019s distinct diagnostic features. Second, dissemination of project results will stir further research to determine if this is an isolated event or is becoming a general phenomenon, and thus a broad concern for Antarctic ecosystems. Third, assays the project develops to detect the disease will enhance research infrastructure. Finally, work will broaden the nation\u2019s scientific workforce by providing authentic research experiences for high school students and undergraduates from groups underrepresented in scientific research. The overall goal of proposed work is to identify the biological origins of the neoplasia and how it affects cell function and physiology. Aim 1 is to identify the pathogenic agent. Aim 1a is to test the hypothesis that a virus causes the neoplasia by isolating and sequencing viral nucleic acids from neoplasias and from animals that are not visibly affected. Aim 1b is to test neoplasias for bacteria, fungi, protozoa, or invertebrate parasites not present in healthy skin. Aim 2 is to learn how the disease alters the biology of affected cells. Aim 2a is to examine histological sections of affected and control tissues to see if the neoplasias are similar to previously reported skin diseases in temperate water fishes. Aim 2b is to examine the function of neoplastic cells by RNA-seq transcriptomics to identify genes that are differentially expressed in neoplasias and normal skin. Achieving these Aims will advance knowledge by identifying the causes and consequences of an outbreak of neoplasias in Antarctic fish. Proposed work is significant because it is the first to investigate a neoplasia cluster in Antarctic fish. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": -62.3, "geometry": "POINT(-63.8 -64.15)", "instruments": null, "is_usap_dc": true, "keywords": "FIELD SURVEYS; Andvord Bay; Amd/Us; PROTISTS; BENTHIC; FISH; Dallmann Bay; USAP-DC; NSF/USA; AMD", "locations": "Andvord Bay; Dallmann Bay", "north": -63.3, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Postlethwait, John; Varsani, Arvind; Desvignes, Thomas", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS", "repo": "USAP-DC", "repositories": "GenBank; MorphoSource; NCBI SRA; R2R; USAP-DC; ZooBank", "science_programs": null, "south": -65.0, "title": "EAGER: Origin and Physiological Consequences of a Neoplasm Outbreak in Antarctic Fish ", "uid": "p0010221", "west": -65.3}, {"awards": "1937748 Sumner, Dawn", "bounds_geometry": "POINT(163.183333 -77.616667)", "dataset_titles": "Lake Fryxell 2022-2023 benthic microbial mat thickness and number of laminae", "datasets": [{"dataset_uid": "601839", "doi": "10.15784/601839", "keywords": "Antarctica; Cryosphere; Dry Valleys; Lake Fryxell; Laminae; Microbial Mat; Thickness", "people": "Mackey, Tyler; Juarez Rivera, Marisol; Sumner, Dawn; Paul, Ann; Hawes, Ian", "repository": "USAP-DC", "science_program": null, "title": "Lake Fryxell 2022-2023 benthic microbial mat thickness and number of laminae", "url": "https://www.usap-dc.org/view/dataset/601839"}], "date_created": "Wed, 30 Jun 2021 00:00:00 GMT", "description": "Part I: Non-technical summary: This project focuses on understanding annual changes in microbial life that grows on the bottom of Lake Fryxell, Antarctica. Because of its polar latitude, photosynthesis can only occur during the summer months. During summer, photosynthetic bacteria supply communities with energy and oxygen. However, it is unknown how the microbes behave in the dark winter, when observations are not possible. This project will install environmental monitors and light-blocking shades over parts of these communities. The shades will extend winter conditions into the spring to allow researchers to characterize the winter behavior of the microbial communities. Researchers will measure changes in the water chemistry due to microbial activities when the shades are removed and the mats first receive light. Results are expected to provide insights into how organisms interact with and change their environments. The project includes training of graduate students and early career scientists in fieldwork, including scientific ice diving techniques. In addition, the members of the project team will develop a web-based \u201cGuide to Thrive\u201d, which will compile field tips ranging from basic gear use to advanced environmental protection techniques. This will be a valuable resource for group leaders ranging from undergraduate teaching assistants to Antarctic expedition leaders to lead well-planned and tailored field expeditions. Part II: Technical summary: The research team will measure seasonal metabolic and biogeochemical changes in benthic mats using differential gene expression and geochemical gradients. They will identify seasonal phenotypic differences in microbial communities and ecosystem effects induced by spring oxygen production. To do so, researchers will install environmental sensors and opaque shades over mats at three depths in the lake. The following spring, shaded and unshaded mats will be sampled. The shades will then be removed, and changes in pore water O2, H2S, pH, and redox will be measured using microelectrodes. Mats will also be sampled for transcriptomic gene expression analyses at intervals guided by geochemical changes. Pore water will be sampled for nutrient analyses. Field research will be supplemented with laboratory experiments to refine field techniques, gene expression data analysis, and integration of results into a seasonal model of productivity and nitrogen cycling in Lake Fryxell. Results will provide insights into several key priorities for NSF, including how biotic, abiotic and environmental components of the benthic mats interact to affect Antarctic lakes. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 163.183333, "geometry": "POINT(163.183333 -77.616667)", "instruments": null, "is_usap_dc": true, "keywords": "Antarctica; Amd/Us; AMD; USA/NSF; FIELD SURVEYS; ECOSYSTEM FUNCTIONS; Lake Fryxell; USAP-DC; LAKE/POND", "locations": "Antarctica; Lake Fryxell", "north": -77.616667, "nsf_funding_programs": "Antarctic Integrated System Science; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Sumner, Dawn; Mackey, Tyler", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -77.616667, "title": "Seasonal Primary Productivity and Nitrogen Cycling in Photosynthetic Mats, Lake Fryxell, McMurdo Dry Valleys", "uid": "p0010219", "west": 163.183333}, {"awards": "1846837 Bowman, Jeff", "bounds_geometry": null, "dataset_titles": "PRJNA894514; PRJNA901488", "datasets": [{"dataset_uid": "200479", "doi": "", "keywords": null, "people": null, "repository": "NCBI SRA", "science_program": null, "title": "PRJNA894514", "url": "https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA894514"}, {"dataset_uid": "200480", "doi": "", "keywords": null, "people": null, "repository": "NCBI SRA", "science_program": null, "title": "PRJNA901488", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA901488"}], "date_created": "Fri, 25 Jun 2021 00:00:00 GMT", "description": "The coastal Antarctic is undergoing great environmental change. Physical changes in the environment, such as altered sea ice duration and extent, have a direct impact on the phytoplankton and bacteria species which form the base of the marine foodweb. Photosynthetic phytoplankton are the ocean\u0027s primary producers, transforming (fixing) CO2 into organic carbon molecules and providing a source of food for zooplankton and larger predators. When phytoplankton are consumed by zooplankton, or killed by viral attack, they release large amounts of organic carbon and nutrients into the environment. Heterotrophic bacteria must eat other things, and function as \"master recyclers\", consuming these materials and converting them to bacterial biomass which can feed larger organisms such as protists. Some protists are heterotrophs, but others are mixotrophs, able to grow by photosynthesis or heterotrophy. Previous work suggests that by killing and eating bacteria, protists and viruses may regulate bacterial populations, but how these processes are regulated in Antarctic waters is poorly understood. This project will use experiments to determine the rate at which Antarctic protists consume bacteria, and field studies to identify the major bacterial taxa involved in carbon uptake and recycling. In addition, this project will use new sequencing technology to obtain completed genomes for many Antarctic marine bacteria. To place this work in an ecosystem context this project will use microbial diversity data to inform rates associated with key microbial processes within the PALMER ecosystem model. This project addresses critical unknowns regarding the ecological role of heterotrophic marine bacteria in the coastal Antarctic and the top-down controls on bacterial populations. Previous work suggests that at certain times of the year grazing by heterotrophic and mixotrophic protists may meet or exceed bacterial production rates. Similarly, in more temperate waters bacteriophages (viruses) are thought to contribute significantly to bacterial mortality during the spring and summer. These different top-down controls have implications for carbon flow through the marine foodweb, because protists are grazed more efficiently by higher trophic levels than are bacteria. This project will use a combination of grazing experiments and field observations to assess the temporal dynamics of mortality due to temperate bacteriophage and protists. Although many heterotrophic bacterial strains observed in the coastal Antarctic are taxonomically similar to strains from other regions, recent work suggest that they are phylogenetically and genetically distinct. To better understand the ecological function and evolutionary trajectories of key Antarctic marine bacteria, their genomes will be isolated and sequenced. Then, these genomes will be used to improve the predictions of the paprica metabolic inference pipeline, and our understanding of the relationship between heterotrophic bacteria and their major predators in the Antarctic marine environment. Finally, researchers will modify the Regional Test-Bed Model model to enable microbial diversity data to be used to optimize the starting conditions of key parameters, and to constrain the model\u0027s data assimilation methods. There is an extensive education and outreach component to this project that is designed to engage students and the public in diverse activities centered on Antarctic microbiota and marine sciences. A new module on Antarctic marine science will be developed for the popular Sally Ride Science program, and two existing undergraduate courses at UC San Diego will be strengthened with laboratory modules introducing emerging technology, and with cutting-edge polar science. A PhD student and a post-doctoral researcher will be supported by this project. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "Magmatic Volatiles; BACTERIA/ARCHAEA; VIRUSES; USA/NSF; Palmer Station; ECOSYSTEM FUNCTIONS; COMMUNITY DYNAMICS; LABORATORY; Amd/Us; PROTISTS; AMD; USAP-DC", "locations": "Palmer Station", "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Bowman, Jeff; Connors, Elizabeth", "platforms": "OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repo": "NCBI SRA", "repositories": "NCBI SRA", "science_programs": null, "south": null, "title": "CAREER: Understanding microbial heterotrophic processes in coastal Antarctic waters", "uid": "p0010201", "west": null}, {"awards": "1643345 Popp, Brian; 1643466 Hollibaugh, James", "bounds_geometry": "POLYGON((-78.20206667 -64.03195833,-76.785055836 -64.03195833,-75.368045002 -64.03195833,-73.951034168 -64.03195833,-72.534023334 -64.03195833,-71.1170125 -64.03195833,-69.700001666 -64.03195833,-68.282990832 -64.03195833,-66.865979998 -64.03195833,-65.448969164 -64.03195833,-64.03195833 -64.03195833,-64.03195833 -64.554377497,-64.03195833 -65.076796664,-64.03195833 -65.599215831,-64.03195833 -66.121634998,-64.03195833 -66.644054165,-64.03195833 -67.166473332,-64.03195833 -67.688892499,-64.03195833 -68.211311666,-64.03195833 -68.733730833,-64.03195833 -69.25615,-65.448969164 -69.25615,-66.865979998 -69.25615,-68.282990832 -69.25615,-69.700001666 -69.25615,-71.1170125 -69.25615,-72.534023334 -69.25615,-73.951034168 -69.25615,-75.368045002 -69.25615,-76.785055836 -69.25615,-78.20206667 -69.25615,-78.20206667 -68.733730833,-78.20206667 -68.211311666,-78.20206667 -67.688892499,-78.20206667 -67.166473332,-78.20206667 -66.644054165,-78.20206667 -66.121634998,-78.20206667 -65.599215831,-78.20206667 -65.076796664,-78.20206667 -64.554377497,-78.20206667 -64.03195833))", "dataset_titles": "\"Collaborative research: Chemoautotrophy in Antarctic bacterioplankton communities supported by the oxidation of urea-derived nitrogen\"; Expedition data of LMG1801", "datasets": [{"dataset_uid": "200193", "doi": "Not yet assigned", "keywords": null, "people": null, "repository": "BCO-DMO", "science_program": null, "title": "\"Collaborative research: Chemoautotrophy in Antarctic bacterioplankton communities supported by the oxidation of urea-derived nitrogen\"", "url": "https://www.bco-dmo.org/project/775717"}, {"dataset_uid": "200124", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG1801", "url": "https://www.rvdata.us/search/cruise/LMG1801"}], "date_created": "Fri, 18 Dec 2020 00:00:00 GMT", "description": "Part 1: Nitrification is the conversion of ammonium to nitrate by a two-step process involving two different guilds of microorganisms: ammonia- and nitrite-oxidizers. The process is central to the global nitrogen cycle, affecting everything from retention of fertilizer on croplands to removal of excess nitrogen from coastal waters before it can cause blooms of harmful algae. It also produces nitrous oxide, an ozone-destroying, greenhouse gas. The energy derived from both steps of nitrification is used to convert inorganic carbon into microbial biomass. The biomass produced contributes to the overall food web production of the Southern Ocean and may be a particularly important subsidy during winter when low light levels restrict the other major source of biomass, primary production by single-celled plants. This project addresses three fundamental questions about the biology and geochemistry of polar oceans, with a focus on the process of nitrification. The first question the project will address concerns the contribution of chemoautotrophy (based on nitrification) to the overall supply of organic carbon to the food web of the Southern Ocean. Previous measurements indicate that it contributes about 9% to the Antarctic food web on an annual basis, but those measurements did not include the additional production associated with nitrite oxidation. The second question to be addressed is related to the first and concerns the coupling between the steps of the process. The third seeks to determine the significance of the contribution of other sources of nitrogen, (specifically organic nitrogen and urea released by other organisms) to nitrification because these contributions may not be assessed by standard protocols. Measurements made by others suggest that urea in particular might be as important as ammonium to nitrification in polar regions. This project will result in training a postdoctoral researcher and provide undergraduate students opportunities to gain hand-on experience with research on microbial geochemistry. The Palmer LTER (PAL) activities have focused largely on the interaction between ocean climate and the marine food web affecting top predators. Relatively little effort has been devoted to studying processes related to the microbial geochemistry of nitrogen cycling as part of the Palmer Long Term Ecological Research (LTER) program, yet these are a major themes at other sites. This work will contribute substantially to understanding an important aspect of nitrogen cycling and bacterioplankton production in the PAL-LTER study area. The team will be working synergistically and be participating fully in the education and outreach efforts of the Palmer LTER, including making highlights of the findings available for posting to their project web site and participating in any special efforts they have in the area of outreach. Part 2: The proposed work will quantify oxidation rates of 15N supplied as ammonium, urea and nitrite, allowing us to estimate the contribution of urea-derived N and complete nitrification (ammonia to nitrate) to chemoautotrophy and bacterioplankton production in Antarctic coastal waters. The project will compare these estimates to direct measurements of the incorporation of 14C into organic matter the dark for an independent estimate of chemoautotrophy. The team aims to collect samples spanning the water column: from surface water (~10 m), winter water (50-100 m) and circumpolar deep water (\u003e150 m); on a cruise surveying the continental shelf and slope west of the Antarctic Peninsula in the austral summer of 2018. Other samples will be taken to measure the concentrations of nitrate, nitrite, ammonia and urea, for qPCR analysis of the abundance of relevant microorganisms, and for studies of related processes. The project will rely on collaboration with the existing Palmer LTER to ensure that ancillary data (bacterioplankton abundance and production, chlorophyll, physical and chemical variables) will be available. The synergistic activities of this project along with the LTER activities will provide a unique opportunity to assess chemoautotrophy in context of the overall ecosystem?s dynamics- including both primary and secondary production processes.", "east": -64.03195833, "geometry": "POINT(-71.1170125 -66.644054165)", "instruments": null, "is_usap_dc": true, "keywords": "Pal-Lter; NITROGEN; SHIPS; USAP-DC; MARINE ECOSYSTEMS; BACTERIA/ARCHAEA; BIOGEOCHEMICAL CYCLES; Amd/Us; West Antarctic Shelf; USA/NSF; AMD", "locations": "West Antarctic Shelf; Pal-Lter", "north": -64.03195833, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Hollibaugh, James T.; Popp, Brian", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e SHIPS", "repo": "BCO-DMO", "repositories": "BCO-DMO; R2R", "science_programs": null, "south": -69.25615, "title": "Collaborative Research: Chemoautotrophy in Antarctic Bacterioplankton Communities Supported by the Oxidation of Urea-derived Nitrogen", "uid": "p0010150", "west": -78.20206667}, {"awards": "1341736 Adams, Byron", "bounds_geometry": "POLYGON((-177.4099 -84.4661,-177.08229 -84.4661,-176.75468 -84.4661,-176.42707 -84.4661,-176.09946 -84.4661,-175.77185 -84.4661,-175.44424 -84.4661,-175.11663000000001 -84.4661,-174.78902 -84.4661,-174.46141 -84.4661,-174.1338 -84.4661,-174.1338 -84.56828,-174.1338 -84.67045999999999,-174.1338 -84.77264,-174.1338 -84.87482,-174.1338 -84.977,-174.1338 -85.07918,-174.1338 -85.18136,-174.1338 -85.28354,-174.1338 -85.38571999999999,-174.1338 -85.4879,-174.46141 -85.4879,-174.78902 -85.4879,-175.11663000000001 -85.4879,-175.44424 -85.4879,-175.77185 -85.4879,-176.09946 -85.4879,-176.42707 -85.4879,-176.75468 -85.4879,-177.08229 -85.4879,-177.4099 -85.4879,-177.4099 -85.38571999999999,-177.4099 -85.28354,-177.4099 -85.18136,-177.4099 -85.07918,-177.4099 -84.977,-177.4099 -84.87482,-177.4099 -84.77264,-177.4099 -84.67045999999999,-177.4099 -84.56828,-177.4099 -84.4661))", "dataset_titles": "Dataset DS-TAMS: Genetic diversity of Collembola from the Transantarctic Mountains; GenBank accession numbers MN619477 to MN619610; Meteoric 10Be data of soils from the Shackleton Glacier region; Shackleton Glacier region soil water-soluble geochemical data; Shackleton Glacier region water-soluble salt isotopes; Soil invertebrate surveys from the Shackleton Glacier region of Antarctica during the 2017-2018 austral summer", "datasets": [{"dataset_uid": "200258", "doi": "doi:10.6073/pasta/7959821e5f6f8d56d94bb6a26873b3ae", "keywords": null, "people": null, "repository": "EDI", "science_program": null, "title": "Soil invertebrate surveys from the Shackleton Glacier region of Antarctica during the 2017-2018 austral summer", "url": "https://doi.org/10.6073/pasta/7959821e5f6f8d56d94bb6a26873b3ae"}, {"dataset_uid": "200174", "doi": "10.5883/DS-TAMS", "keywords": null, "people": null, "repository": "Barcode of Life Datasystems (BOLD)", "science_program": null, "title": "Dataset DS-TAMS: Genetic diversity of Collembola from the Transantarctic Mountains", "url": "http://dx.doi.org/10.5883/DS-TAMS"}, {"dataset_uid": "200175", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "GenBank accession numbers MN619477 to MN619610", "url": "https://www.ncbi.nlm.nih.gov/nuccore/MN619477"}, {"dataset_uid": "601418", "doi": "10.15784/601418", "keywords": "Antarctica; Geochemistry; Shackleton Glacier", "people": "Gardner, Christopher B.; Lyons, W. Berry; Diaz, Melisa A.", "repository": "USAP-DC", "science_program": null, "title": "Shackleton Glacier region soil water-soluble geochemical data", "url": "https://www.usap-dc.org/view/dataset/601418"}, {"dataset_uid": "601419", "doi": "10.15784/601419", "keywords": "Antarctica; Geochemistry; Nitrate; Shackleton Glacier; Stable Isotopes; Sulfate; Transantarctic Mountains", "people": "Lyons, W. Berry; Gardner, Christopher B.; Diaz, Melisa A.", "repository": "USAP-DC", "science_program": null, "title": "Shackleton Glacier region water-soluble salt isotopes", "url": "https://www.usap-dc.org/view/dataset/601419"}, {"dataset_uid": "601421", "doi": "10.15784/601421", "keywords": "Antarctica; Be-10; Beryllium-10; Cosmogenic Radionuclides; Geochemistry; Geomorphology; Shackleton Glacier; Surface Exposure Dates", "people": "Diaz, Melisa A.", "repository": "USAP-DC", "science_program": null, "title": "Meteoric 10Be data of soils from the Shackleton Glacier region", "url": "https://www.usap-dc.org/view/dataset/601421"}], "date_created": "Mon, 02 Nov 2020 00:00:00 GMT", "description": "The project will characterize the functional, taxonomic, biotic and abiotic drivers of soil ecosystems in the Trans Antarctic Mountains (one of the most remote and harsh terrestrial landscapes on the planet). The work will utilize new high-throughput DNA and RNA sequencing technologies to identify members of the microbial communities and determine if the microbial community structures are independent of local environmental heterogeneities. In addition the project will determine if microbial diversity and function are correlated with time since the last glacial maximum (LGM). The expected results will greatly contribute to our knowledge regarding rates of microbial succession and help define the some of the limits to life and life-maintaining processes on Earth. The project will analyze genomes and RNA derived from these genomes to describe the relationships between biodiversity and ecosystem functioning from soils above and below LGM elevations and to correlate these with the environmental drivers associated with their development during the last ~18,000 years. The team will identify the taxonomic diversity and the functional genetic composition within a broad suite of soil biota and examine their patterns of assembly and distribution within the framework of their geological legacies. The project will mentor participants from undergraduate students to postdoctoral researchers and prepare them to effectively engage in research to meet their career aspirations. The project will contribute to ongoing public education efforts through relationships with K-12 teachers and administrators- to include University-Public School partnerships. Less formal activities include public lecture series and weblogs aimed at providing information on Antarctic polar desert ecosystems to the general public. Targeted classrooms near each PI\u0027s institution will participate in online, real-time discussions about current topics in Antarctic ecosystems research.", "east": -174.1338, "geometry": "POINT(-175.77185 -84.977)", "instruments": null, "is_usap_dc": true, "keywords": "FIELD INVESTIGATION; LABORATORY; AMD; Amd/Us; USA/NSF; TERRESTRIAL ECOSYSTEMS; Transantarctic Mountains; USAP-DC", "locations": "Transantarctic Mountains", "north": -84.4661, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Earth Sciences", "paleo_time": null, "persons": "Adams, Byron; Fierer, Noah; Wall, Diana; Diaz, Melisa A.; Gardner, Christopher B.; Lyons, W. Berry", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION; OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repo": "EDI", "repositories": "Barcode of Life Datasystems (BOLD); EDI; NCBI GenBank; USAP-DC", "science_programs": null, "south": -85.4879, "title": "Collaborative Research: The Role of Glacial History on the Structure and Functioning of Ecological Communities in the Shackleton Glacier Region of the Transantarctic Mountains", "uid": "p0010140", "west": -177.4099}, {"awards": "0732711 Smith, Craig; 0732917 McCormick, Michael; 0732450 Van Dover, Cindy; 0732983 Vernet, Maria", "bounds_geometry": "POLYGON((-60.5 -63.1,-59.99 -63.1,-59.48 -63.1,-58.97 -63.1,-58.46 -63.1,-57.95 -63.1,-57.44 -63.1,-56.93 -63.1,-56.42 -63.1,-55.91 -63.1,-55.4 -63.1,-55.4 -63.29,-55.4 -63.48,-55.4 -63.67,-55.4 -63.86,-55.4 -64.05,-55.4 -64.24,-55.4 -64.43,-55.4 -64.62,-55.4 -64.81,-55.4 -65,-55.91 -65,-56.42 -65,-56.93 -65,-57.44 -65,-57.95 -65,-58.46 -65,-58.97 -65,-59.48 -65,-59.99 -65,-60.5 -65,-60.5 -64.81,-60.5 -64.62,-60.5 -64.43,-60.5 -64.24,-60.5 -64.05,-60.5 -63.86,-60.5 -63.67,-60.5 -63.48,-60.5 -63.29,-60.5 -63.1))", "dataset_titles": "LARISSA: Impact of ice-shelf loss on geochemical profiles and microbial community composition in marine sediments of the Larsen A embayment, Antarctic Peninsula; NBP1001 cruise data; NBP1203 cruise data; Species Abundance Data from the Larsen Ice Shelf Ice acquired during R/V Nathaniel B. Palmer expedition NBP1203", "datasets": [{"dataset_uid": "601073", "doi": "10.15784/601073", "keywords": "Antarctica; Antarctic Peninsula; Chemistry:ice; Chemistry:Ice; Geochemistry; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Ice Core Records; LARISSA; Microbiology", "people": "McCormick, Michael", "repository": "USAP-DC", "science_program": null, "title": "LARISSA: Impact of ice-shelf loss on geochemical profiles and microbial community composition in marine sediments of the Larsen A embayment, Antarctic Peninsula", "url": "https://www.usap-dc.org/view/dataset/601073"}, {"dataset_uid": "000142", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "NBP1001 cruise data", "url": "https://www.rvdata.us/search/cruise/NBP1001"}, {"dataset_uid": "000143", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "NBP1203 cruise data", "url": "https://www.rvdata.us/search/cruise/NBP1203"}, {"dataset_uid": "601304", "doi": null, "keywords": "Antarctica; Antarctic Peninsula; Biota; Box Corer; LARISSA; Larsen Ice Shelf; Macrofauna; Megafauna; NBP1203; Oceans; R/v Nathaniel B. Palmer; Seafloor Sampling; Species Abundance", "people": "Smith, Craig", "repository": "USAP-DC", "science_program": "LARISSA", "title": "Species Abundance Data from the Larsen Ice Shelf Ice acquired during R/V Nathaniel B. Palmer expedition NBP1203", "url": "https://www.usap-dc.org/view/dataset/601304"}], "date_created": "Fri, 09 Oct 2020 00:00:00 GMT", "description": "A profound transformation in ecosystem structure and function is occurring in coastal waters of the western Weddell Sea, with the collapse of the Larsen B ice shelf. This transformation appears to be yielding a redistribution of energy flow between chemoautotrophic and photosynthetic production, and to be causing the rapid demise of the extraordinary seep ecosystem discovered beneath the ice shelf. This event provides an ideal opportunity to examine fundamental aspects of ecosystem transition associated with climate change. We propose to test the following hypotheses to elucidate the transformations occurring in marine ecosystems as a consequence of the Larsen B collapse: (1) The biogeographic isolation and sub-ice shelf setting of the Larsen B seep has led to novel habitat characteristics, chemoautotrophically dependent taxa and functional adaptations. (2) Benthic communities beneath the former Larsen B ice shelf are fundamentally different from assemblages at similar depths in the Weddell sea-ice zone, and resemble oligotrophic deep-sea communities. Larsen B assemblages are undergoing rapid change. (3) The previously dark, oligotrophic waters of the Larsen B embayment now support a thriving phototrophic community, with production rates and phytoplankton composition similar to other productive areas of the Weddell Sea. To document rapid changes occurring in the Larsen B ecosystem, we will use a remotely operated vehicle, shipboard samplers, and moored sediment traps. We will characterize microbial, macrofaunal and megafaunal components of the seep community; evaluate patterns of surface productivity, export flux, and benthic faunal composition in areas previously covered by the ice shelf, and compare these areas to the open sea-ice zone. These changes will be placed within the geological, glaciological and climatological context that led to ice-shelf retreat, through companion research projects funded in concert with this effort. Together these projects will help predict the likely consequences of ice-shelf collapse to marine ecosystems in other regions of Antarctica vulnerable to climate change. The research features international collaborators from Argentina, Belgium, Canada, Germany, Spain and the United Kingdom. The broader impacts include participation of a science writer; broadcast of science segments by members of the Jim Lehrer News Hour (Public Broadcasting System); material for summer courses in environmental change; mentoring of graduate students and postdoctoral fellows; and showcasing scientific activities and findings to students and public through podcasts.", "east": -55.4, "geometry": "POINT(-57.95 -64.05)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "NBP1203; USAP-DC; Amd/Us; LARISSA; Larsen Ice Shelf; Species Abundance Data; R/V NBP; Antarctic Peninsula; NBP1001; USA/NSF; AMD; Antarctica; MARINE ECOSYSTEMS", "locations": "Antarctica; Antarctic Peninsula; Larsen Ice Shelf", "north": -63.1, "nsf_funding_programs": "Antarctic Integrated System Science; Antarctic Organisms and Ecosystems; Antarctic Integrated System Science; Antarctic Organisms and Ecosystems; Antarctic Integrated System Science; Antarctic Integrated System Science", "paleo_time": null, "persons": "McCormick, Michael; Vernet, Maria; Van Dover, Cindy; Smith, Craig", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repo": "USAP-DC", "repositories": "R2R; USAP-DC", "science_programs": "LARISSA", "south": -65.0, "title": "Collaborative Research in IPY: Abrupt Environmental Change in the Larsen Ice Shelf System, a Multidisciplinary Approach - Marine Ecosystems.", "uid": "p0010135", "west": -60.5}, {"awards": "1644187 Tulaczyk, Slawek", "bounds_geometry": "POLYGON((161 -76.9,161.75 -76.9,162.5 -76.9,163.25 -76.9,164 -76.9,164.75 -76.9,165.5 -76.9,166.25 -76.9,167 -76.9,167.75 -76.9,168.5 -76.9,168.5 -77.04,168.5 -77.18,168.5 -77.32,168.5 -77.46,168.5 -77.6,168.5 -77.74,168.5 -77.88,168.5 -78.02,168.5 -78.16,168.5 -78.3,167.75 -78.3,167 -78.3,166.25 -78.3,165.5 -78.3,164.75 -78.3,164 -78.3,163.25 -78.3,162.5 -78.3,161.75 -78.3,161 -78.3,161 -78.16,161 -78.02,161 -77.88,161 -77.74,161 -77.6,161 -77.46,161 -77.32,161 -77.18,161 -77.04,161 -76.9))", "dataset_titles": "ANTAEM project airborne EM resistivity data from McMurdo Region", "datasets": [{"dataset_uid": "601373", "doi": "10.15784/601373", "keywords": "Antarctica; Dry Valleys; Hydrology; Ice Shelf; McMurdo; Permafrost", "people": "Tulaczyk, Slawek", "repository": "USAP-DC", "science_program": null, "title": "ANTAEM project airborne EM resistivity data from McMurdo Region", "url": "https://www.usap-dc.org/view/dataset/601373"}], "date_created": "Sun, 13 Sep 2020 00:00:00 GMT", "description": "In Antarctica, millions of years of freezing have led to the development of hundreds of meters of thick permafrost (i.e., frozen ground). Recent research demonstrated that this slow freezing has trapped and concentrated water into local and regional briny aquifers, many times more salty than seawater. Because salt depresses the freezing point of water, these saline brines are able to persist as liquid water at temperatures well below the normal freezing point of freshwater. Such unusual groundwater systems may support microbial life, supply nutrients to coastal ocean and ice-covered lakes, and influence motion of glaciers. These briny aquifers also represent potential terrestrial analogs for deep life habitats on other planets, such as Mars, and provide a testing ground for the search for extraterrestrial water. Whereas much effort has been invested in understanding the physics, chemistry, and biology of surface and near-surface waters in cold polar regions, it has been comparably difficult to investigate deep subsurface aquifers in such settings. Airborne ElectroMagnetics (AEM) subsurface imaging provides an efficient way for mapping salty groundwater. An international collaboration with the University of Aarhus in Denmark will enable knowledge and skill transfer in AEM techniques that will enhance US polar research capabilities and provide US undergraduates and graduate students with unique training experiences. This project will survey over 1000 km2 of ocean and land near McMurdo Station in Antarctica, and will reveal if cold polar deserts hide a subsurface pool of liquid water. This will have significant implications for understanding cold polar glaciers, ice-covered lakes, frozen ground, and polar microbiology as well as for predictions of their response to future change. Improvements in permafrost mapping techniques and understanding of permafrost and of underlying groundwaters will benefit human use of high polar regions in the Antarctic and the Arctic. The project will provide the first integrative system-scale overview of subsurface water distribution and hydrological connectivity in a partly ice-free coastal region of Antarctica, the McMurdo Dry Valleys. Liquid water is relatively scarce in this environment but plays an outsized role by influencing, and integrating, biological, biogeochemical, glaciological, and geological processes. Whereas surface hydrology and its role in ecosystem processes has been thoroughly studied over the last several decades, it has been difficult to map out and characterize subsurface water reservoirs and to understand their interactions with regional lakes, glaciers, and coastal waters. The proposed project builds on the \"proof-of-concept\" use of AEM technology in 2011. Improvements in sensor and data processing capabilities will result in about double the depth of penetration of the subsurface during the new data collection when compared to the 2011 proof-of-concept survey, which reached depths of 300-400m. The first field season will focus on collecting deep soundings with a ground-based system in key locations where: (i) independent constraints on subsurface structure exist from past drilling projects, and (ii) the 2011 resistivity dataset indicates the need for deeper penetration and high signal-to-noise ratios achievable only with a ground-based system. The regional airborne survey will take place during the second field season and will yield subsurface electrical resistivity data from across several valleys of different sizes and different ice cover fractions.", "east": 168.5, "geometry": "POINT(164.75 -77.6)", "instruments": null, "is_usap_dc": true, "keywords": "FROZEN GROUND; GLACIERS/ICE SHEETS; HELICOPTER; GROUND WATER; RIVERS/STREAMS; Dry Valleys", "locations": "Dry Valleys", "north": -76.9, "nsf_funding_programs": "Antarctic Integrated System Science; Antarctic Earth Sciences", "paleo_time": null, "persons": "Tulaczyk, Slawek; Mikucki, Jill", "platforms": "AIR-BASED PLATFORMS \u003e ROTORCRAFT/HELICOPTER \u003e HELICOPTER", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -78.3, "title": "Collaborative Research: Antarctic Airborne ElectroMagnetics (ANTAEM) - Revealing Subsurface Water in Coastal Antarctica", "uid": "p0010129", "west": 161.0}, {"awards": "1620976 Johnson, Sarah", "bounds_geometry": "POLYGON((160 -77,160.3 -77,160.6 -77,160.9 -77,161.2 -77,161.5 -77,161.8 -77,162.1 -77,162.4 -77,162.7 -77,163 -77,163 -77.1,163 -77.2,163 -77.3,163 -77.4,163 -77.5,163 -77.6,163 -77.7,163 -77.8,163 -77.9,163 -78,162.7 -78,162.4 -78,162.1 -78,161.8 -78,161.5 -78,161.2 -78,160.9 -78,160.6 -78,160.3 -78,160 -78,160 -77.9,160 -77.8,160 -77.7,160 -77.6,160 -77.5,160 -77.4,160 -77.3,160 -77.2,160 -77.1,160 -77))", "dataset_titles": "GenBank Sequence Read Archive with accession numbers SRR8217969 - SRR8217976 and project accession PRJNA506221", "datasets": [{"dataset_uid": "200164", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "GenBank Sequence Read Archive with accession numbers SRR8217969 - SRR8217976 and project accession PRJNA506221", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA506221/"}], "date_created": "Tue, 01 Sep 2020 00:00:00 GMT", "description": "Despite recent advances, we still know little about how life and its traces persist in extremely harsh conditions. What survival strategies do cells employ when pushed to their limit? Using a new technique, this project will investigate whether Antarctic paleolakes harbor \"microbial seed banks,\" or caches of viable microbes adapted to past paleoenvironments that could help transform our understanding of how cells survive over ancient timescales. Findings from this investigation could also illuminate novel DNA repair pathways with possible biomedical and biotechnology applications and help to refine life detection strategies for Mars. The project will bring Antarctic research to Georgetown University\u0027s campus for the first time, providing training opportunities in cutting edge analytical techniques for multiple students and a postdoctoral fellow. The field site will be the McMurdo Dry Valleys, which provide an unrivaled opportunity to investigate fundamental questions about the persistence of microbial life. Multiple lines of evidence, from interbedded and overlying ashfall deposits to parameterized models, suggest that the large-scale landforms there have remained essentially fixed as far back as the middle of the Miocene Epoch (i.e., ~8 million years ago). This geologic stability, coupled with geographic isolation and a steady polar climate, mean that biological activity has probably undergone few qualitative changes over the last one to two million years. The team will sample paleolake facies using sterile techniques from multiple Dry Valleys sites and extract DNA from entombed organic material. Genetic material will then be sequenced using Pacific Biosciences\u0027 Single Molecule, Real-Time DNA sequencing technology, which sequences native DNA as opposed to amplified DNA, thereby eliminating PCR primer bias, and enables read lengths that have never before been possible. The data will be analyzed with a range of bioinformatic techniques, with results that stand to impact our understanding of cell biology, Antarctic paleobiology, microbiology and biogeography, biotechnology, and planetary science.", "east": 163.0, "geometry": "POINT(161.5 -77.5)", "instruments": null, "is_usap_dc": true, "keywords": "ECOSYSTEM FUNCTIONS; COMMUNITY DYNAMICS; BACTERIA/ARCHAEA; CYANOBACTERIA (BLUE-GREEN ALGAE); LABORATORY; Dry Valleys", "locations": "Dry Valleys", "north": -77.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Earth Sciences", "paleo_time": null, "persons": "Johnson, Sarah", "platforms": "OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repo": "NCBI GenBank", "repositories": "NCBI GenBank", "science_programs": null, "south": -78.0, "title": "EAGER: Single-Molecule DNA Sequencing of Antarctic Paleolakes", "uid": "p0010125", "west": 160.0}, {"awards": "1543450 Countway, Peter", "bounds_geometry": "POLYGON((-66 -63,-65.7 -63,-65.4 -63,-65.1 -63,-64.8 -63,-64.5 -63,-64.2 -63,-63.9 -63,-63.6 -63,-63.3 -63,-63 -63,-63 -63.3,-63 -63.6,-63 -63.9,-63 -64.2,-63 -64.5,-63 -64.8,-63 -65.1,-63 -65.4,-63 -65.7,-63 -66,-63.3 -66,-63.6 -66,-63.9 -66,-64.2 -66,-64.5 -66,-64.8 -66,-65.1 -66,-65.4 -66,-65.7 -66,-66 -66,-66 -65.7,-66 -65.4,-66 -65.1,-66 -64.8,-66 -64.5,-66 -64.2,-66 -63.9,-66 -63.6,-66 -63.3,-66 -63))", "dataset_titles": "Biogenic Sulfur Samples from Station E (Palmer Station, Antarctica) and Associated Incubation Experiments; Dissolved Inorganic Nutrient Samples from Station E (Palmer Station, Antarctica) and Associated Incubation Experiments ; Dissolved Organic Carbon (DOC) and Total Dissolved Nitrogen (TDN) Samples from Station E (Palmer Station, Antarctica) and Associated Incubation Experiments; Flow Cytometry Samples from Station E (Palmer Station, Antarctica) and Associated Incubation Experiments; Heterotrophic Bacterial Production Samples from Station E (Palmer Station, Antarctica) and Associated Incubation Experiments; Western Antarctic Peninsula plankton raw sequence reads", "datasets": [{"dataset_uid": "200337", "doi": "", "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "Western Antarctic Peninsula plankton raw sequence reads", "url": "https://dataview.ncbi.nlm.nih.gov/object/PRJNA870587?reviewer=bmud2tbbrqbus79i2n2hb83uio"}, {"dataset_uid": "601645", "doi": "10.15784/601645", "keywords": "Antarctica; Nitrate; Nitrite; Palmer Station; Phosphate", "people": "Countway, Peter; Matrai, Patricia", "repository": "USAP-DC", "science_program": null, "title": "Dissolved Inorganic Nutrient Samples from Station E (Palmer Station, Antarctica) and Associated Incubation Experiments ", "url": "https://www.usap-dc.org/view/dataset/601645"}, {"dataset_uid": "601647", "doi": "10.15784/601647", "keywords": "Antarctica; Palmer Station; Phytoplankton", "people": "Countway, Peter; Matrai, Patricia", "repository": "USAP-DC", "science_program": null, "title": "Flow Cytometry Samples from Station E (Palmer Station, Antarctica) and Associated Incubation Experiments", "url": "https://www.usap-dc.org/view/dataset/601647"}, {"dataset_uid": "601646", "doi": "10.15784/601646", "keywords": "Antarctica; Carbon; Dissolved Organic Carbon; Nitrogen; Palmer Station; TDN; Total Dissolved Nitrogen", "people": "Matrai, Patricia; Countway, Peter", "repository": "USAP-DC", "science_program": null, "title": "Dissolved Organic Carbon (DOC) and Total Dissolved Nitrogen (TDN) Samples from Station E (Palmer Station, Antarctica) and Associated Incubation Experiments", "url": "https://www.usap-dc.org/view/dataset/601646"}, {"dataset_uid": "601648", "doi": "10.15784/601648", "keywords": "Antarctica; Biota; Dimethyl Sulfide; Dimethylsulfoniopropionate; Dimethylsulfoxide; DMSP; DMSP Lyase; Palmer Station", "people": "Matrai, Patricia; Countway, Peter", "repository": "USAP-DC", "science_program": null, "title": "Biogenic Sulfur Samples from Station E (Palmer Station, Antarctica) and Associated Incubation Experiments", "url": "https://www.usap-dc.org/view/dataset/601648"}, {"dataset_uid": "601644", "doi": "10.15784/601644", "keywords": "3H-Leu; Antarctica; Bacteria; Biota; DMSP; Heterotrophic Bacterial Production; Palmer Station", "people": "Matrai, Patricia; Countway, Peter", "repository": "USAP-DC", "science_program": null, "title": "Heterotrophic Bacterial Production Samples from Station E (Palmer Station, Antarctica) and Associated Incubation Experiments", "url": "https://www.usap-dc.org/view/dataset/601644"}], "date_created": "Sat, 01 Aug 2020 00:00:00 GMT", "description": "The Southern Ocean in the vicinity of Antarctica is a region characterized by seasonally-driven marine phytoplankton blooms that are often dominated by microalgal species which produce large amounts of dimethylsulfoniopropionate (DMSP). DMSP can be converted to the compound dimethylsulfide (DMS) which is a molecule that can escape into the atmosphere where it is known to have strong condensation properties that are involved in regional cloud formation. Production of DMSP can influence the diversity and composition of microbial assemblages in seawater and the types and activities of microbes in the seawater will likely affect the magnitude of DMSP\\DMS production. The proposal aims to examine the role of DMSP in structuring the microbial communities in Antarctic waters and how this structuring may influence DMSP cycling. The project will leverage the Antarctic research to introduce concepts and data linking microbial diversity and biogeochemistry to a range of audiences (including high school and undergraduate students in Maine). The project will also engage teacher and students in rural K-8 schools and will allow a collaboration with a science writer and illustrator who will join the team in the field. The writer will use the southern ocean experience as the setting for a poster and a book about the proposed research and the scientists studying extreme environments. The project will examine (1) the extent to which the cycling of DMSP in southern ocean waters influences the composition and diversity of bacterial and protistan assemblages; (2) conversely, whether the composition and diversity of southern ocean protistan and bacterial assemblages influence the magnitude and rates of DMSP cycling; (3) the expression of DMSP degradation genes by marine bacteria seasonally and in response to additions of DMSP; and, to synthesize these results by quantifying (4) the microbial networks resulting from the presence of DMSP-producers and DMSP-consumers along with their predators, all involved in the cycling of DMSP in southern ocean waters. The work will be accomplished by conducting continuous growth experiments with DMSP-amended natural samples during field sampling of different microbial communities present in summer and fall. Data from the molecular (such as 16S/ 18S tag sequences, DMSP-cycle gene transcripts) and biogeochemical (such as biogenic sulfur cycling, bacterial production, microbial biomass) investigations will be integrated via network analysis.", "east": -63.0, "geometry": "POINT(-64.5 -64.5)", "instruments": null, "is_usap_dc": true, "keywords": "USAP-DC; COMMUNITY DYNAMICS; FIELD INVESTIGATION; AMD; PLANKTON; Amd/Us; BIOGEOCHEMICAL CYCLES; Palmer Station; USA/NSF", "locations": "Palmer Station", "north": -63.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Countway, Peter", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION", "repo": "GenBank", "repositories": "GenBank; USAP-DC", "science_programs": null, "south": -66.0, "title": "Microbial Community Structure and Expression of Functional Genes Involved in the Seasonal Cycling of DMSP in the Southern Ocean", "uid": "p0010120", "west": -66.0}, {"awards": "1543396 Christner, Brent; 1543453 Lyons, W. Berry; 1543537 Priscu, John; 1543441 Fricker, Helen; 1543347 Rosenheim, Brad; 1543405 Leventer, Amy", "bounds_geometry": "POLYGON((-163.611 -84.33543,-162.200034 -84.33543,-160.789068 -84.33543,-159.378102 -84.33543,-157.967136 -84.33543,-156.55617 -84.33543,-155.145204 -84.33543,-153.734238 -84.33543,-152.323272 -84.33543,-150.912306 -84.33543,-149.50134 -84.33543,-149.50134 -84.3659157,-149.50134 -84.3964014,-149.50134 -84.4268871,-149.50134 -84.4573728,-149.50134 -84.4878585,-149.50134 -84.5183442,-149.50134 -84.5488299,-149.50134 -84.5793156,-149.50134 -84.6098013,-149.50134 -84.640287,-150.912306 -84.640287,-152.323272 -84.640287,-153.734238 -84.640287,-155.145204 -84.640287,-156.55617 -84.640287,-157.967136 -84.640287,-159.378102 -84.640287,-160.789068 -84.640287,-162.200034 -84.640287,-163.611 -84.640287,-163.611 -84.6098013,-163.611 -84.5793156,-163.611 -84.5488299,-163.611 -84.5183442,-163.611 -84.4878585,-163.611 -84.4573728,-163.611 -84.4268871,-163.611 -84.3964014,-163.611 -84.3659157,-163.611 -84.33543))", "dataset_titles": "Antarctica - PI Continuous - GZ01-WIS_GroundingZone_01 P.S. - GPS/GNSS Observations Dataset; Antarctica - PI Continuous - GZ13-WIS_GroundingZone_13 P.S. - GPS/GNSS Observations Dataset; Antarctica - PI Continuous - LA02-WIS_LAKES_02 P.S. - GPS/GNSS Observations Dataset; Antarctica - PI Continuous - LA06-WIS_LAKES_06 P.S. - GPS/GNSS Observations Dataset; Antarctica - PI Continuous - LA07-WIS_LAKES_07 P.S. - GPS/GNSS Observations Dataset; Antarctica - PI Continuous - LA09-WIS_LAKES_09 P.S. - GPS/GNSS Observations Dataset; Bistatic Radar Sounding of Whillans Ice Stream, Antarctica and Store Glacier, Greenland; CTD data from Mercer Subglacial Lake and access borehole; Discrete bulk sediment properties data from Mercer Subglacial Lake; Isotopic data from Whillans Ice Stream grounding zone, West Antarctica; Mercer Subglacial Lake radiocarbon and stable isotope data ; Mercer Subglacial Lake (SLM) microbial composition: 16S rRNA genes (Sequence Read Archive; BioProject: PRJNA790995); Mercer Subglacial Lake (SLM) noble gas and isotopic data; Mercer Subglacial Lake water column viral metagenomic sequencing; Salsa sediment cores; Sediment porewater properties data from Mercer Subglacial Lake; Water column biogeochemical data from Mercer Subglacial Lake", "datasets": [{"dataset_uid": "200215", "doi": "10.7283/C503-KS23", "keywords": null, "people": null, "repository": "UNAVCO", "science_program": null, "title": "Antarctica - PI Continuous - LA06-WIS_LAKES_06 P.S. - GPS/GNSS Observations Dataset", "url": "https://www.unavco.org/data/doi/10.7283/C503-KS23"}, {"dataset_uid": "200212", "doi": "10.7283/PT0Q-JB95", "keywords": null, "people": null, "repository": "UNAVCO", "science_program": null, "title": "Antarctica - PI Continuous - GZ01-WIS_GroundingZone_01 P.S. - GPS/GNSS Observations Dataset", "url": "https://www.unavco.org/data/doi/10.7283/PT0Q-JB95"}, {"dataset_uid": "200213", "doi": "10.7283/F7BB-JH05", "keywords": null, "people": null, "repository": "UNAVCO", "science_program": null, "title": "Antarctica - PI Continuous - GZ13-WIS_GroundingZone_13 P.S. - GPS/GNSS Observations Dataset", "url": "https://www.unavco.org/data/doi/10.7283/F7BB-JH05"}, {"dataset_uid": "200214", "doi": "10.7283/YW8Z-TK03", "keywords": null, "people": null, "repository": "UNAVCO", "science_program": null, "title": "Antarctica - PI Continuous - LA02-WIS_LAKES_02 P.S. - GPS/GNSS Observations Dataset", "url": "https://www.unavco.org/data/doi/10.7283/YW8Z-TK03"}, {"dataset_uid": "200216", "doi": "10.7283/F8NH-CV04", "keywords": null, "people": null, "repository": "UNAVCO", "science_program": null, "title": "Antarctica - PI Continuous - LA07-WIS_LAKES_07 P.S. - GPS/GNSS Observations Dataset", "url": "https://www.unavco.org/data/doi/10.7283/F8NH-CV04"}, {"dataset_uid": "601498", "doi": "10.15784/601498", "keywords": "Antarctica; Mercer Subglacial Lake; Noble Gas", "people": "Lyons, W. Berry; Gardner, Christopher B.", "repository": "USAP-DC", "science_program": null, "title": "Mercer Subglacial Lake (SLM) noble gas and isotopic data", "url": "https://www.usap-dc.org/view/dataset/601498"}, {"dataset_uid": "200282", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Mercer Subglacial Lake (SLM) microbial composition: 16S rRNA genes (Sequence Read Archive; BioProject: PRJNA790995)", "url": "https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA790995"}, {"dataset_uid": "601657", "doi": "10.15784/601657", "keywords": "Antarctica; Conductivity; CTD; Depth; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Hot Water Drill; Mercer Subglacial Lake; Physical Properties; SALSA; Subglacial Lake; Temperature", "people": "Priscu, John; Rosenheim, Brad; Leventer, Amy; Dore, John", "repository": "USAP-DC", "science_program": null, "title": "CTD data from Mercer Subglacial Lake and access borehole", "url": "https://www.usap-dc.org/view/dataset/601657"}, {"dataset_uid": "200246", "doi": "", "keywords": null, "people": null, "repository": "OSU-MGR", "science_program": null, "title": "Salsa sediment cores", "url": "https://osu-mgr.org"}, {"dataset_uid": "601360", "doi": "10.15784/601360", "keywords": "Antarctica; Radiocarbon; Sediment; Whillans Ice Stream", "people": "Venturelli, Ryan A", "repository": "USAP-DC", "science_program": "WISSARD", "title": "Isotopic data from Whillans Ice Stream grounding zone, West Antarctica", "url": "https://www.usap-dc.org/view/dataset/601360"}, {"dataset_uid": "601672", "doi": "10.15784/601672", "keywords": "Antarctica; Isotope; Mercer Subglacial Lake; Radiocarbon; Subglacial Lake", "people": "Venturelli, Ryan; Rosenheim, Brad", "repository": "USAP-DC", "science_program": null, "title": "Mercer Subglacial Lake radiocarbon and stable isotope data ", "url": "https://www.usap-dc.org/view/dataset/601672"}, {"dataset_uid": "200342", "doi": null, "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "Mercer Subglacial Lake water column viral metagenomic sequencing", "url": "https://www.ncbi.nlm.nih.gov/biosample/32811410"}, {"dataset_uid": "200217", "doi": "10.7283/3JMY-Y504", "keywords": null, "people": null, "repository": "UNAVCO", "science_program": null, "title": "Antarctica - PI Continuous - LA09-WIS_LAKES_09 P.S. - GPS/GNSS Observations Dataset", "url": "https://www.unavco.org/data/doi/10.7283/3JMY-Y504"}, {"dataset_uid": "601664", "doi": "10.15784/601664", "keywords": "Antarctica; Gas; Geochemistry; Glacier; Glaciology; Mercer Subglacial Lake; Methane; SALSA; Sediment Core; West Antarctic Ice Sheet", "people": "Michaud, Alexander; Dore, John; Science Team, SALSA; Steigmeyer, August; Tranter, Martyn; Skidmore, Mark", "repository": "USAP-DC", "science_program": null, "title": "Sediment porewater properties data from Mercer Subglacial Lake", "url": "https://www.usap-dc.org/view/dataset/601664"}, {"dataset_uid": "601663", "doi": "10.15784/601663", "keywords": "Antarctica; Carbon; Cell Counts; Geochemistry; Glacier; Glaciers/ice Sheet; Glaciers/Ice Sheet; Mercer Subglacial Lake; Microbes; Nutrients; SALSA; Stable Isotopes; Trace Elements; West Antarctic Ice Sheet", "people": "Steigmeyer, August; Hawkings, Jon; Skidmore, Mark; Dore, John; Science Team, SALSA; Priscu, John; Tranter, Martyn; Barker, Joel; Li, Wei", "repository": "USAP-DC", "science_program": null, "title": "Water column biogeochemical data from Mercer Subglacial Lake", "url": "https://www.usap-dc.org/view/dataset/601663"}, {"dataset_uid": "601661", "doi": "10.15784/601661", "keywords": "Antarctica; Carbon; Glacier; Glaciers/ice Sheet; Glaciers/Ice Sheet; Iron; Mercer Subglacial Lake; Mineralogy; Particle Size; Physical Properties; SALSA; Sediment Core; Sulfur; West Antarctic Ice Sheet", "people": "Campbell, Timothy; Dore, John; Michaud, Alexander; Hawkings, Jon; Skidmore, Mark; Tranter, Martyn; Venturelli, Ryan A; Science Team, SALSA", "repository": "USAP-DC", "science_program": null, "title": "Discrete bulk sediment properties data from Mercer Subglacial Lake", "url": "https://www.usap-dc.org/view/dataset/601661"}, {"dataset_uid": "601472", "doi": "10.15784/601472", "keywords": "Antarctica; Bistatic Radar; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; GPS Data; Greenland; Lake Whillans; Radar; Store Glacier; Whillans Ice Stream; WISSARD", "people": "Christoffersen, Poul; Peters, Sean; Bienert, Nicole; Siegfried, Matthew; Schroeder, Dustin; Dawson, Eliza; MacKie, Emma", "repository": "USAP-DC", "science_program": "WISSARD", "title": "Bistatic Radar Sounding of Whillans Ice Stream, Antarctica and Store Glacier, Greenland", "url": "https://www.usap-dc.org/view/dataset/601472"}], "date_created": "Thu, 16 Jul 2020 00:00:00 GMT", "description": "The Antarctic subglacial environment remains one of the least explored regions on Earth. This project will examine the physical and biological characteristics of Subglacial Lake Mercer, a lake that lies 1200m beneath the West Antarctic Ice Sheet. This study will address key questions relating to the stability of the ice sheet, the subglacial hydrological system, and the deep-cold subglacial biosphere. The education and outreach component aims to widely disseminate results to the scientific community and to the general public through short films, a blog, and a website. Subglacial Lake Mercer is one of the larger hydrologically active lakes in the southern basin of the Whillans Ice Plain, West Antarctica. It receives about 25 percent of its water from East Antarctica with the remainder originating from West Antarctica, is influenced by drain/fill cycles in a lake immediately upstream (Subglacial Lake Conway), and lies about 100 km upstream of the present grounding line of the Ross Ice Shelf. This site will yield information on the history of the Whillans and Mercer Ice Streams, and on grounding line migration. The integrated study will include direct sampling of basal ice, water, and sediment from the lake in concert with surface geophysical surveys over a three-year period to define the hydrological connectivity among lakes on the Whillans Ice Plain and their flow paths to the sea. The geophysical surveys will furnish information on subglacial hydrology, aid the site selection for hot-water drilling, and provide spatial context for interpreting findings. The hot-water-drilled boreholes will be used to collect basal ice samples, provide access for direct measurement of subglacial physical, chemical, and biological conditions in the water column and sediments, and to explore the subglacial water cavities using a remotely operated vehicle equipped with sensors, cameras, and sampling equipment. Data collected from this study will address the overarching hypothesis \"Contemporary biodiversity and carbon cycling in hydrologically-active subglacial environments associated with the Mercer and Whillans ice streams are regulated by the mineralization and cycling of relict marine organic matter and through interactions among ice, rock, water, and sediments\". The project will be undertaken by a collaborative team of scientists, with expertise in microbiology, biogeochemistry, hydrology, geophysics, glaciology, marine geology, paleoceanography, and science communication.", "east": -149.50134, "geometry": "POINT(-156.55617 -84.4878585)", "instruments": null, "is_usap_dc": true, "keywords": "SEDIMENTS; Antarctica; ISOTOPES; Subglacial Lake; USAP-DC; VIRUSES; PALEOCLIMATE RECONSTRUCTIONS; BACTERIA/ARCHAEA; LABORATORY; Radiocarbon; Whillans Ice Stream; AMD; SALSA; ECOSYSTEM FUNCTIONS; RADIOCARBON; FIELD INVESTIGATION; ICE MOTION; Mercer Ice Stream; Amd/Us; USA/NSF; GLACIERS/ICE SHEETS", "locations": "Antarctica; Mercer Ice Stream; Whillans Ice Stream", "north": -84.33543, "nsf_funding_programs": "Antarctic Glaciology; Antarctic Integrated System Science; Antarctic Glaciology; Antarctic Integrated System Science; Antarctic Glaciology; Antarctic Glaciology; Antarctic Glaciology; Antarctic Instrumentation and Support; Antarctic Integrated System Science; Antarctic Integrated System Science; Antarctic Integrated System Science; Antarctic Integrated System Science", "paleo_time": null, "persons": "Rosenheim, Brad; Fricker, Helen; Priscu, John; Leventer, Amy; Dore, John; Lyons, W. Berry; Christner, Brent", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION; OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repo": "UNAVCO", "repositories": "GenBank; NCBI GenBank; OSU-MGR; UNAVCO; USAP-DC", "science_programs": null, "south": -84.640287, "title": "Collaborative Research: Subglacial Antarctic Lakes Scientific Access (SALSA): Integrated Study of Carbon Cycling in Hydrologically-active Subglacial Environments", "uid": "p0010119", "west": -163.611}, {"awards": "1745341 Sumner, Dawn", "bounds_geometry": "POLYGON((161.595 -77.527,161.5953 -77.527,161.5956 -77.527,161.5959 -77.527,161.5962 -77.527,161.5965 -77.527,161.5968 -77.527,161.5971 -77.527,161.5974 -77.527,161.5977 -77.527,161.598 -77.527,161.598 -77.5271,161.598 -77.5272,161.598 -77.5273,161.598 -77.5274,161.598 -77.5275,161.598 -77.5276,161.598 -77.5277,161.598 -77.5278,161.598 -77.5279,161.598 -77.528,161.5977 -77.528,161.5974 -77.528,161.5971 -77.528,161.5968 -77.528,161.5965 -77.528,161.5962 -77.528,161.5959 -77.528,161.5956 -77.528,161.5953 -77.528,161.595 -77.528,161.595 -77.5279,161.595 -77.5278,161.595 -77.5277,161.595 -77.5276,161.595 -77.5275,161.595 -77.5274,161.595 -77.5273,161.595 -77.5272,161.595 -77.5271,161.595 -77.527))", "dataset_titles": "GP0191362, Gp0191371; JAAXLU000000000, JAAXLT000000000", "datasets": [{"dataset_uid": "200151", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "JAAXLU000000000, JAAXLT000000000", "url": "https://www.ncbi.nlm.nih.gov/nuccore/JAAXLU000000000"}, {"dataset_uid": "200152", "doi": "", "keywords": null, "people": null, "repository": "IMG Gold", "science_program": null, "title": "GP0191362, Gp0191371", "url": "https://gold.jgi.doe.gov/study?id=Gs0127369"}], "date_created": "Mon, 22 Jun 2020 00:00:00 GMT", "description": "Atmospheric oxygen rose suddenly approximately 2.4 billion years ago after Cyanobacteria evolved the ability to produce oxygen through photosynthesis (oxygenic photosynthesis). This change permanently altered the future of life on Earth, yet little is known about the evolutionary processes leading to it. The Melainabacteria were first discovered in 2013 and are closely related non-photosynthetic relatives of the first group of organisms capable of oxygenic photosynthesis. This project will utilize existing data on metagenomes from microbial mats in Lake Vanda, an ice-covered lake in Antarctica where many sequences of Melainabacteria have been previously identified. From this genetic information, the project aims to assess the metabolic capabilities of these Melainabacteria and identify their potential ecological roles. The project will additionally evaluate the evolutionary relationships among the Cyanobacteria and Melainabacteria and closely related organisms that will allow an advancement in understanding of the evolutionary path that lead to oxygenic photosynthesis on Earth. The project will focus on extracting evolutionary information from the genomic data of Melainabacteria and Sericytochromatia, recently-described groups closely related to but basal to the Cyanobacteria. The characterization of novel members of these groups in samples from Lake Vanda, Antarctica, will provide insights into the path and processes involved in the evolution of oxygenic photosynthesis. The research will focus on assessing the metabolic capabilities of Melainabacteri, deriving the evolutionary relationships among Melainabacteria and Cyanobacteria and reconstructing potential evolutionary pathways leading to oxygenic photosynthesis. The project will focus on 12 metagenomes where the researchers expect to obtain genomes for at least the eight most abundant Melainabacteria in the dataset. Melainabacteria bins will be annotated and preliminary metabolic pathways will be constructed. The project will utilize full-length sequences of marker genes from across the bacterial domain with a particular focus on taxa that are oxygenic or anoxygenic phototrophs and use the marker genes, to build a rooted \"backbone\" tree. Incomplete or short sequences from the metagenomes will be added to the tree using the Evolutionary Placement Algorithm. The researchers will also build a corresponding phylogenetic tree using a Bayesian framework and compare their topologies. By doing so, the project aims to improve the understanding of the evolution of oxygenic photosynthesis, which caused the most significant change in Earth\u0027s surface chemistry. Specifically, they will document a significantly broader metabolic diversity within the Melainabacteria than has been previously identified, gain significant insights into their metabolic evolution, their evolutionary relationships with the Cyanobacteria, and the evolutionary steps leading to the origin of oxygenic photosynthesis. This research will have the overall effect of constraining key evolutionary processes in the origin of oxygenic photosynthesis. It will provide the foundation for future studies by indicating where a genomic record of the evolution of oxygenic photosynthesis may be preserved. Results will also be shared with middle school children through the development of scientific lesson plans in collaboration with teachers. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 161.598, "geometry": "POINT(161.5965 -77.5275)", "instruments": null, "is_usap_dc": true, "keywords": "USAP-DC; FIELD INVESTIGATION; CYANOBACTERIA (BLUE-GREEN ALGAE); Lake Vanda; LABORATORY; LAKE/POND; Genetic Analysis", "locations": "Lake Vanda", "north": -77.527, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Sumner, Dawn; Eisen, Jonathan; Tazi, Loubna", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION; OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repo": "NCBI GenBank", "repositories": "IMG Gold; NCBI GenBank", "science_programs": null, "south": -77.528, "title": "Evolution of Oxygenic Photosynthesis as Preserved in Melainabacterial Genomes from Lake Vanda, Antarctica", "uid": "p0010112", "west": 161.595}, {"awards": "1443578 Schmidt, Steven", "bounds_geometry": "POLYGON((161.5 -77.5,161.7 -77.5,161.9 -77.5,162.1 -77.5,162.3 -77.5,162.5 -77.5,162.7 -77.5,162.9 -77.5,163.1 -77.5,163.3 -77.5,163.5 -77.5,163.5 -77.53,163.5 -77.56,163.5 -77.59,163.5 -77.62,163.5 -77.65,163.5 -77.68,163.5 -77.71,163.5 -77.74,163.5 -77.77,163.5 -77.8,163.3 -77.8,163.1 -77.8,162.9 -77.8,162.7 -77.8,162.5 -77.8,162.3 -77.8,162.1 -77.8,161.9 -77.8,161.7 -77.8,161.5 -77.8,161.5 -77.77,161.5 -77.74,161.5 -77.71,161.5 -77.68,161.5 -77.65,161.5 -77.62,161.5 -77.59,161.5 -77.56,161.5 -77.53,161.5 -77.5))", "dataset_titles": "16S and 18S amplicon sequencing of Antarctic cryoconite holes; Genomes of Antarctic ssDNA viruses (GenBank accession numbers MN311489-MN311492 and MN328267-MN328291); Metadata from samples (in the process of submitting to EDI; will update with DOI once completed); Microbial species-area relationships in Antarctic cryoconite holes; Soil microbial communities of a mountain landscape, McMurdo Dry Valleys, Antarctica", "datasets": [{"dataset_uid": "200279", "doi": "", "keywords": null, "people": null, "repository": "GitHub", "science_program": null, "title": "Metadata from samples (in the process of submitting to EDI; will update with DOI once completed)", "url": "https://github.com/pacificasommers/Cryoconite-metadata"}, {"dataset_uid": "200081", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "16S and 18S amplicon sequencing of Antarctic cryoconite holes", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA480849/"}, {"dataset_uid": "200280", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Soil microbial communities of a mountain landscape, McMurdo Dry Valleys, Antarctica", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA721735/"}, {"dataset_uid": "200281", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Microbial species-area relationships in Antarctic cryoconite holes", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA668398/"}, {"dataset_uid": "200084", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Genomes of Antarctic ssDNA viruses (GenBank accession numbers MN311489-MN311492 and MN328267-MN328291)", "url": ""}], "date_created": "Fri, 01 Nov 2019 00:00:00 GMT", "description": "Cryoconite holes are pockets of life completely encased in otherwise barren glacial ice. These pockets of life form when dust blown onto the ice melts a small, largely isolated hole that can function as its own tiny ecosystem. This dust can contain microorganisms such as bacteria, algae, or microscopic animals. The microorganisms within the hole interact and carry out functions typical of a larger ecosystem, such as a forest. Cryoconite holes are especially important in extreme cold environments such as the Antarctic Dry Valleys, where they function as repositories of life. Because cryoconite holes are mostly enclosed and persist for years, they can be tracked over time to test fundamental scientific questions about how communities of interacting organisms develop to become fully functioning ecosystems. This project will sample existing and experimentally created cryoconite holes to understand how these ecosystems develop and to what degree random processes (such as which organisms get there first) affect the final community composition and functioning. The results will not only improve our understanding of how microbial communities assemble and affect the functioning of microecosystems such as cryoconite holes, but also how the processes of community assembly affect functioning of larger ecosystems, such as forests. A better understanding of community establishment, development, and response to abiotic factors are essential to forecasting ecological responses to environmental change. It is essential to unravel the links between community assembly, biodiversity, and nutrient cycling across numerous ecosystems because these are critical factors determining ecological responses to environmental change. The unique, largely isolated nature of cryoconite holes provides an experimental system that will advance fundamental understanding of the processes (e.g., stochastic dynamics such as dispersal limitation, assembly order, and ecological drift) driving community assembly. This project will use a field sampling campaign and a number of manipulative experiments to test a hypothesis that unites theory in community and ecosystem ecology: the degree to which stochastic processes guide microbial community assembly and affects regional patterns in biodiversity and ecosystem processes. Cryoconite holes will be sampled to compare community composition, environmental factors, and ecosystem functioning between hydrologically connected and isolated holes. New cryoconite holes will also be constructed and monitored over the course of two growing seasons to specifically alter assembly order and community size, thereby pairing a unique manipulative experiment with field surveys to address questions with relevance to the Antarctic and beyond. Amplicon sequencing, metagenomics, microscopy, sensitive environmental chemistry methods, and photosynthesis and respiration measurements will be used to test a series of sub-hypotheses that relate stochasticity to patterns in regional biodiversity, heterogeneity in environmental factors, and ecosystem processes.", "east": 163.5, "geometry": "POINT(162.5 -77.65)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "GLACIERS; Antarctica; USAP-DC; FIELD INVESTIGATION", "locations": "Antarctica", "north": -77.5, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Schmidt, Steven; Cawley, Kaelin; Fountain, Andrew", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION", "repo": "GitHub", "repositories": "GitHub; NCBI GenBank", "science_programs": null, "south": -77.8, "title": "Collaborative Research: Stochasticity and Cryoconite Community Assembly and Function", "uid": "p0010063", "west": 161.5}, {"awards": "1644073 DiTullio, Giacomo; 1643684 Saito, Mak", "bounds_geometry": "POLYGON((-180 -72,-173.6 -72,-167.2 -72,-160.8 -72,-154.4 -72,-148 -72,-141.6 -72,-135.2 -72,-128.8 -72,-122.4 -72,-116 -72,-116 -72.7,-116 -73.4,-116 -74.1,-116 -74.8,-116 -75.5,-116 -76.2,-116 -76.9,-116 -77.6,-116 -78.3,-116 -79,-122.4 -79,-128.8 -79,-135.2 -79,-141.6 -79,-148 -79,-154.4 -79,-160.8 -79,-167.2 -79,-173.6 -79,180 -79,178 -79,176 -79,174 -79,172 -79,170 -79,168 -79,166 -79,164 -79,162 -79,160 -79,160 -78.3,160 -77.6,160 -76.9,160 -76.2,160 -75.5,160 -74.8,160 -74.1,160 -73.4,160 -72.7,160 -72,162 -72,164 -72,166 -72,168 -72,170 -72,172 -72,174 -72,176 -72,178 -72,-180 -72))", "dataset_titles": "Algal pigment concentrations from the Ross Sea; Biogenic silica concentrations from the Ross Sea; NBP1801 Expedition data; Nutrients from NBP18-01 CICLOPS", "datasets": [{"dataset_uid": "601205", "doi": "10.15784/601205", "keywords": "Antarctica; Chemistry:fluid; Chemistry:Fluid; Chlorophyll; Chromatography; Liquid Chromatograph; Oceans; Ross Sea; R/v Nathaniel B. Palmer; Sea Water; Seawater Measurements; Southern Ocean; Water Measurements; Water Samples", "people": "Ditullio, Giacomo", "repository": "USAP-DC", "science_program": null, "title": "Algal pigment concentrations from the Ross Sea", "url": "https://www.usap-dc.org/view/dataset/601205"}, {"dataset_uid": "601225", "doi": "10.15784/601225", "keywords": "Antarctica; Biogenic Silica; Biogenic Silica Concentrations; Chemistry:Water; Geochemistry; NBP1801; Oceans; Ross Sea; R/v Nathaniel B. Palmer; Sea Water; Southern Ocean; Spectroscopy; Water Measurements; Water Samples", "people": "Ditullio, Giacomo; Schanke, Nicole", "repository": "USAP-DC", "science_program": null, "title": "Biogenic silica concentrations from the Ross Sea", "url": "https://www.usap-dc.org/view/dataset/601225"}, {"dataset_uid": "200056", "doi": "10.7284/907753", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "NBP1801 Expedition data", "url": "https://www.rvdata.us/search/cruise/NBP1801"}, {"dataset_uid": "601428", "doi": "10.15784/601428", "keywords": "Amundsen Sea; Antarctica; NBP1801; Nitrate; Nitrite; Nutrients; Phosphate; Ross Sea; R/v Nathaniel B. Palmer; Silicic Acid; Terra Nova Bay", "people": "Saito, Mak", "repository": "USAP-DC", "science_program": null, "title": "Nutrients from NBP18-01 CICLOPS", "url": "https://www.usap-dc.org/view/dataset/601428"}], "date_created": "Thu, 08 Aug 2019 00:00:00 GMT", "description": "Phytoplankton blooms in the coastal waters of the Ross Sea, Antarctica are typically dominated by either diatoms or Phaeocystis Antarctica (a flagellated algae that often can form large colonies in a gelatinous matrix). The project seeks to determine if an association of bacterial populations with Phaeocystis antarctica colonies can directly supply Phaeocystis with Vitamin B12, which can be an important co-limiting micronutrient in the Ross Sea. The supply of an essential vitamin coupled with the ability to grow at lower iron concentrations may put Phaeocystis at a competitive advantage over diatoms. Because Phaeocystis cells can fix more carbon than diatoms and Phaeocystis are not grazed as efficiently as diatoms, the project will help in refining understanding of carbon dynamics in the region as well as the basis of the food web webs. Such understanding also has the potential to help refine predictive ecological models for the region. The project will conduct public outreach activities and will contribute to undergraduate and graduate research. Engagement of underrepresented students will occur during summer student internships. A collaboration with Italian Antarctic researchers, who have been studying the Terra Nova Bay ecosystem since the 1980s, aims to enhance the project and promote international scientific collaborations. The study will test whether a mutualistic symbioses between attached bacteria and Phaeocystis provides colonial cells a mechanism for alleviating chronic Vitamin B12 co-limitation effects thereby conferring them with a competitive advantage over diatom communities. The use of drifters in a time series study will provide the opportunity to track in both space and time a developing algal bloom in Terra Nova Bay and to determine community structure and the physiological nutrient status of microbial populations. A combination of flow cytometry, proteomics, metatranscriptomics, radioisotopic and stable isotopic labeling experiments will determine carbon and nutrient uptake rates and the role of bacteria in mitigating potential vitamin B12 and iron limitation. Membrane inlet and proton transfer reaction mass spectrometry will also be used to estimate net community production and release of volatile organic carbon compounds that are climatically active. Understanding how environmental parameters can influence microbial community dynamics in Antarctic coastal waters will advance an understanding of how changes in ocean stratification and chemistry could impact the biogeochemistry and food web dynamics of Southern Ocean ecosystems.", "east": 160.0, "geometry": "POINT(-158 -75.5)", "instruments": null, "is_usap_dc": true, "keywords": "BIOGEOCHEMICAL CYCLES; NBP1801; Amd/Us; USA/NSF; USAP-DC; NUTRIENTS; PIGMENTS; CHLOROPHYLL; R/V NBP; Ross Sea; AMD", "locations": "Ross Sea", "north": -72.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Integrated System Science; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "DiTullio, Giacomo; Lee, Peter", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repo": "USAP-DC", "repositories": "R2R; USAP-DC", "science_programs": null, "south": -79.0, "title": "Collaborative Research: Cobalamin and Iron Co-Limitation Of Phytoplankton Species in Terra Nova Bay", "uid": "p0010045", "west": -116.0}, {"awards": "1745053 Salvatore, Mark; 1744849 Sokol, Eric; 1744785 Barrett, John", "bounds_geometry": "POLYGON((162.92 -77.56,162.971 -77.56,163.022 -77.56,163.073 -77.56,163.124 -77.56,163.175 -77.56,163.226 -77.56,163.277 -77.56,163.328 -77.56,163.379 -77.56,163.43 -77.56,163.43 -77.571,163.43 -77.582,163.43 -77.593,163.43 -77.604,163.43 -77.615,163.43 -77.626,163.43 -77.637,163.43 -77.648,163.43 -77.659,163.43 -77.67,163.379 -77.67,163.328 -77.67,163.277 -77.67,163.226 -77.67,163.175 -77.67,163.124 -77.67,163.073 -77.67,163.022 -77.67,162.971 -77.67,162.92 -77.67,162.92 -77.659,162.92 -77.648,162.92 -77.637,162.92 -77.626,162.92 -77.615,162.92 -77.604,162.92 -77.593,162.92 -77.582,162.92 -77.571,162.92 -77.56))", "dataset_titles": "McMurdo Dry Valleys LTER: Microbial mat biomass and Normalized Difference Vegetation Index (NDVI) values from Lake Fryxell Basin, Antarctica, January 2018", "datasets": [{"dataset_uid": "200344", "doi": "10.6073/pasta/9acbbde9abc1e013f8c9fd9c383327f4", "keywords": null, "people": null, "repository": "EDI", "science_program": null, "title": "McMurdo Dry Valleys LTER: Microbial mat biomass and Normalized Difference Vegetation Index (NDVI) values from Lake Fryxell Basin, Antarctica, January 2018", "url": "https://portal.edirepository.org/nis/mapbrowse?packageid=knb-lter-mcm.263.1"}], "date_created": "Wed, 03 Jul 2019 00:00:00 GMT", "description": "Microbial mats are found throughout the McMurdo Dry Valleys where summer snowmelt provides liquid water that allows these mats to flourish. Researchers have long studied the environmental conditions microbial mats need to grow. Despite these efforts, it has been difficult to develop a broad picture of these unique ecosystems. Recent advances in satellite technology now provide researchers an exciting new tool to study these special Antarctic ecosystems from space using the unique spectral signatures associated with microbial mats. This new technology not only offers the promise that microbial mats can be mapped and studied from space, this research will also help protect these delicate environments from potentially harmful human impacts that can occur when studying them from the ground. This project will use satellite imagery and spectroscopic techniques to identify and map microbial mat communities and relate their properties and distributions to both field and lab-based measurements. This research provides an exciting new tool to help document and understand the distribution of a major component of the Antarctic ecosystem in the McMurdo Dry Valleys. The goal of this project is to establish quantitative relationships between spectral signatures derived from orbit and the physiological status and biogeochemical properties of microbial mat communities in Taylor Valley, Antarctica, as measured by field and laboratory analyses on collected samples. The goal wioll be met by (1) refining atmospheric correction techniques using in situ radiometric rectification to derive accurate surface spectra; (2) collecting multispectral orbital images concurrent with in situ sampling and spectral measurements in the field to ensure temporal comparability; (3) measuring sediment, water, and microbial mat samples for organic and inorganic carbon content, essential biogeochemical nutrients, and chlorophyll-a to determine relevant mat characteristics; and (4) quantitatively associating these laboratory-derived characteristics with field-derived and orbital spectral signatures and parameters. The result of this work will be a more robust quantitative link between the distribution of microbial mat communities and their biogeochemical properties to landscape-scale spectral signatures. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 163.43, "geometry": "POINT(163.175 -77.615)", "instruments": null, "is_usap_dc": true, "keywords": "RIVERS/STREAM; CYANOBACTERIA (BLUE-GREEN ALGAE); USAP-DC; Taylor Valley; INFRARED IMAGERY; WORLDVIEW-2; WORLDVIEW-3; Antarctica; FIELD INVESTIGATION; Amd/Us; ACTIVE LAYER", "locations": "Antarctica; Taylor Valley", "north": -77.56, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Salvatore, Mark; Barrett, John; Sokol, Eric", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION; SPACE-BASED PLATFORMS \u003e EARTH OBSERVATION SATELLITES \u003e WORLDVIEW \u003e WORLDVIEW-2; SPACE-BASED PLATFORMS \u003e EARTH OBSERVATION SATELLITES \u003e WORLDVIEW \u003e WORLDVIEW-3", "repo": "EDI", "repositories": "EDI", "science_programs": null, "south": -77.67, "title": "COLLABORATIVE RESEARCH: Remote Characterization of Microbial Mats in Taylor Valley, Antarctica, through In Situ Sampling and Spectral Validation", "uid": "p0010036", "west": 162.92}, {"awards": "1642570 Thurber, Andrew", "bounds_geometry": "POINT(166.666 -77.8)", "dataset_titles": "Microbial community composition of the Cinder Cones Cold Seep", "datasets": [{"dataset_uid": "200035", "doi": "DOI:10.1575/1912/bco-dmo.756997.1", "keywords": null, "people": null, "repository": "BCO-DMO", "science_program": null, "title": "Microbial community composition of the Cinder Cones Cold Seep", "url": "https://www.bco-dmo.org/dataset/756997"}], "date_created": "Fri, 24 May 2019 00:00:00 GMT", "description": "Methane is a potent greenhouse gas that is naturally emitted into the oceans by geologic seeps and microbial production. Based on studies of persistent deep-sea seeps at mid- and northern latitudes, researchers have learned that bacteria and archaea can create a \"sediment filter\" that oxidizes methane prior to its release. Antarctica is thought to contain large reservoirs of organic carbon buried beneath its ice which could a quantity of methane equivalent to all of the permafrost in the Arctic and yet we know almost nothing about the methane oxidizing microbes in this region. How these microbial communities develop and potentially respond to fluctuations in methane levels is an under-explored avenue of research. A bacterial mat was recently discovered at 78 degrees south, suggesting the possible presence of a methane seep, and associated microbial communities. This project will explore this environment in detail to assess the levels and origin of methane, and the nature of the microbial ecosystem present. An expansive bacterial mat appeared and/or was discovered at 78 degrees south in 2011. This site, near McMurdo Station Antarctica, has been visited since the mid-1960s, but this mat was not observed until 2011. The finding of this site provides an unusual opportunity to study an Antarctic marine benthic habitat with active methane cycling and to examine the dynamics of recruitment and community succession of seep fauna including bacteria, archaea, protists and metazoans. This project will collect the necessary baseline data to facilitate further studies of Antarctic methane cycling. The concentration and source of methane will be determined at this site and at potentially analogous sites in McMurdo Sound. In addition to biogeochemical characterization of the sites, molecular analysis of the microbial community will quantify the time scales on which bacteria and archaea respond to methane input and provide information on rates of community development and succession in the Southern Ocean. Project activities will facilitate the training of at least one graduate student and results will be shared at both local and international levels. A female graduate student will be mentored as part of this project and data collected will form part of her dissertation. Lectures will be given in K-12 classrooms in Oregon to excite students about polar science. National and international audiences will be reached through blogs and presentations at a scientific conference. The PI\u0027s previous blogs have been used by K-12 classrooms as part of their lesson plans and followed in over 65 countries.", "east": 166.666, "geometry": "POINT(166.666 -77.8)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "Sea Floor; USAP-DC; Ross Sea; BACTERIA/ARCHAEA; NOT APPLICABLE", "locations": "Ross Sea; Sea Floor", "north": -77.8, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Thurber, Andrew", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "BCO-DMO", "repositories": "BCO-DMO", "science_programs": null, "south": -77.8, "title": "EAGER: Elucidating the Antarctic Methane Cycle at the Cinder Cones Reducing Habitat.", "uid": "p0010030", "west": 166.666}, {"awards": "1544526 Omelon, Christopher", "bounds_geometry": "POLYGON((160 -76.5,160.37 -76.5,160.74 -76.5,161.11 -76.5,161.48 -76.5,161.85 -76.5,162.22 -76.5,162.59 -76.5,162.96 -76.5,163.33 -76.5,163.7 -76.5,163.7 -76.63,163.7 -76.76,163.7 -76.89,163.7 -77.02,163.7 -77.15,163.7 -77.28,163.7 -77.41,163.7 -77.54,163.7 -77.67,163.7 -77.8,163.33 -77.8,162.96 -77.8,162.59 -77.8,162.22 -77.8,161.85 -77.8,161.48 -77.8,161.11 -77.8,160.74 -77.8,160.37 -77.8,160 -77.8,160 -77.67,160 -77.54,160 -77.41,160 -77.28,160 -77.15,160 -77.02,160 -76.89,160 -76.76,160 -76.63,160 -76.5))", "dataset_titles": null, "datasets": null, "date_created": "Thu, 09 May 2019 00:00:00 GMT", "description": "Cryptoendoliths are organisms that colonize microscopic cavities of rocks, which give them protection and allow them to inhabit extreme environments, such as the cold, arid desert of the Dry Valleys of Antarctica. Fossilized cryptoendoliths preserve the forms and features of organisms from the past and thus provide a unique opportunity to study the organisms? life histories and environments. To study this fossil record, there needs to be a better understanding of what environmental conditions allow these fossils to form. A climate gradient currently exists in the Dry Valleys that allows us to study living, dead, and fossilized cryptoendoliths from mild to increasingly harsh environments; providing insight to the limits of life and how these fossils are formed. This project will develop instruments to detect the biological activity of the live microorganisms and conduct laboratory experiments to determine the environmental limits of their survival. The project also will characterize the chemical and structural features of the living, dead, and fossilized cryptoendoliths to understand how they become fossilized. Knowing how microorganisms are preserved as fossils in cold and dry environments like Antarctica can help to refine methods that can be used to search for and identify evidence for extraterrestrial life in similar habitats on planets such as Mars. This project includes training of graduate and undergraduate students. Little is known about cryptoendolithic microfossils and their formation processes in cold, arid terrestrial habitats of the Dry Valleys of Antarctica, where a legacy of activity is discernible in the form of biosignatures including inorganic materials and microbial fossils that preserve and indicate traces of past biological activity. The overarching goals of the proposed work are: (1) to determine how rates of microbial respiration and biodegradation of organic matter control microbial fossilization; and (2) to characterize microbial fossils and their living counterparts to elucidate mechanisms for fossilization. Using samples collected across an increasingly harsher (more cold and dry) climatic gradient that encompasses living, dead, and fossilized cryptoendolithic microorganisms, the proposed work will: (1) develop an instrument to be used in the field that can measure small concentrations of CO2 in cryptoendolithic habitats in situ; (2) conduct incubation experiments to target variations in microbial activity in samples containing living, dead, and fossilized microorganisms as well as limits to microbial activity by measuring CO2 evolution and delta13C signatures; and (3) use a suite of microscopy techniques (CLSM, cryo-SEM, FIB-SEM, \u00b5-XFM) to correlate laboratory experimental evidence for microbial viability and activity and to identify the chemical and morphological characteristics of biosignatures and microbial fossils. A metagenomic survey of microbial communities in these samples will be used to characterize differences in diversity, identify if specific microorganisms (e.g. prokaryotes, eukaryotes) are more capable of surviving under these harsh climatic conditions, and to corroborate microscopic observations of the viability states of these microorganisms.", "east": 163.7, "geometry": "POINT(161.85 -77.15)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "USAP-DC; Antarctica; NOT APPLICABLE; TERRESTRIAL ECOSYSTEMS", "locations": "Antarctica", "north": -76.5, "nsf_funding_programs": "Antarctic Earth Sciences", "paleo_time": null, "persons": "Omelon, Christopher; Breecker, Daniel; Bennett, Philip", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repositories": null, "science_programs": null, "south": -77.8, "title": "Activity, Preservation and Fossilization of Cryptoendolithic Microorganisms in Antarctica", "uid": "p0010028", "west": 160.0}, {"awards": "1543412 Reinfelder, John", "bounds_geometry": null, "dataset_titles": "16S rRNA gene libraries of krill gut microbial communities; Microbial gene libraries of krill gut microbial communities", "datasets": [{"dataset_uid": "200024", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Microbial gene libraries of krill gut microbial communities", "url": "https://nam02.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fbioproject%2F531145\u0026amp;data=02%7C01%7Creinfeld%40envsci.rutgers.edu%7C7e30a0192dc748ab271408d6b9d57d08%7Cb92d2b234d35447093ff69aca6632ffe%7C1%7C0%7C636900723909188941\u0026amp;sdata=G6cNg4bBHzeikrWSCYITcT6XS3NLWwjQ1yNdwtrALPc%3D\u0026amp;reserved=0"}, {"dataset_uid": "601171", "doi": "10.15784/601171", "keywords": "Antarctica; Biota; Krill; LTER Palmer Station; Microbiome; Oceans; Southern Ocean", "people": "Reinfelder, John", "repository": "USAP-DC", "science_program": "LTER", "title": "16S rRNA gene libraries of krill gut microbial communities", "url": "https://www.usap-dc.org/view/dataset/601171"}], "date_created": "Sun, 31 Mar 2019 00:00:00 GMT", "description": "Marine food webs can concentrate monomethylmercury (MMHg), a neurotoxin in mammals, in upper trophic level consumers. Despite their remoteness, coastal Antarctic marine ecosystems accumulate and biomagnify MMHg to levels observed at lower latitudes and in the Arctic. Marine sediments and other anoxic habitats in the oceans are typical areas where methylation of mercury occurs and these are likely places where MMHg is being produced. Krill, and more specifically their digestive tracts, may be a previously unaccounted for site where the production of MMHg may be occurring in the Antarctic. If monomethylmercury production is occurring in krill, current views regarding bioaccumulation in the food web and processes leading to the production and accumulation of mercury in the Antarctic Ocean could be better informed, if not transformed. This project will conduct a preliminary assessment of the krill gut microbiomes, the microbiome\u0027s genomic content and potential for production of monomethyl mercury by detecting the genes involved in mercury transformations. By analyzing the krill gut microbiome, the project will provide insights regarding animal-microbe interactions and their potential role in globally important biogeochemical cycles. This project will conduct a preliminary assessment of the krill gut microbiomes, the microbiomes genomic content and potential for production of monomethylmercury. The diversity and metabolic profiles of microorganisms in krill digestive tracts will be evaluated using massively parallel Illumina DNA sequencing technology to produce 16S rRNA gene libraries and assembled whole metagenomes. The project will also quantify the abundance and expression of Hg methylation genes, hgcAB, and identify their taxonomic affiliations in the microbiome communities. Environmental metagenomes, 16S rRNA gene inventories produced from this project will provide the polar science community with valuable databases and experimental tools with which to examine coastal Antarctic microbial ecology and biogeochemistry. The project will seek to provide a wider window into the diversity of extremophile microbial communities and the identification of potentially unique and useful bioactive compounds. In addition to public education and outreach. This project will train graduate students and provide educational and outreach opportunities at the participating institutions", "east": -68.2816, "geometry": "POINT(-69.09295 -66.8017)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "USAP-DC; Antarctica; NOT APPLICABLE; BACTERIA/ARCHAEA", "locations": "Antarctica", "north": -65.8708, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Schaefer, Jeffra; Reinfelder, John; Barkar, T.", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "NCBI GenBank", "repositories": "NCBI GenBank; USAP-DC", "science_programs": "LTER", "south": -67.7326, "title": "Methylmercury in Antarctic Krill Microbiomes", "uid": "p0010023", "west": -69.9043}, {"awards": "1656344 Bowman, Jeff", "bounds_geometry": "POLYGON((-64.1 -64.75,-64.08 -64.75,-64.06 -64.75,-64.04 -64.75,-64.02 -64.75,-64 -64.75,-63.98 -64.75,-63.96 -64.75,-63.94 -64.75,-63.92 -64.75,-63.9 -64.75,-63.9 -64.775,-63.9 -64.8,-63.9 -64.825,-63.9 -64.85,-63.9 -64.875,-63.9 -64.9,-63.9 -64.925,-63.9 -64.95,-63.9 -64.975,-63.9 -65,-63.92 -65,-63.94 -65,-63.96 -65,-63.98 -65,-64 -65,-64.02 -65,-64.04 -65,-64.06 -65,-64.08 -65,-64.1 -65,-64.1 -64.975,-64.1 -64.95,-64.1 -64.925,-64.1 -64.9,-64.1 -64.875,-64.1 -64.85,-64.1 -64.825,-64.1 -64.8,-64.1 -64.775,-64.1 -64.75))", "dataset_titles": "\r\nMetadata accompanying BioProject SUB4579142 ; Western Antarctic Peninsula Marine Metatranscriptomes Sep 29 2018", "datasets": [{"dataset_uid": "200010", "doi": "", "keywords": null, "people": null, "repository": "NCBI SRA", "science_program": null, "title": "Western Antarctic Peninsula Marine Metatranscriptomes Sep 29 2018", "url": "https://submit.ncbi.nlm.nih.gov/subs/sra/SUB4579142/overview"}, {"dataset_uid": "601153", "doi": "", "keywords": "Antarctic; Antarctica; Bacteria; Bacteria Production; Biota; Chlorophyll; LTER Palmer Station; Primary Production; Sea Ice; Southern Ocean", "people": "Bowman, Jeff", "repository": "USAP-DC", "science_program": null, "title": "\r\nMetadata accompanying BioProject SUB4579142 ", "url": "https://www.usap-dc.org/view/dataset/601153"}], "date_created": "Thu, 31 Jan 2019 00:00:00 GMT", "description": "This EAGER project will compare gene expression patterns in the planktonic communities under ice covers that form in coastal embayment\u0027s in the Antarctic Peninsula. Previous efforts taking advantage of unique ice conditions in November and December of 2015 allowed researchers to conduct an experiment to examine the role of sea ice cover on microbial carbon and energy transfer during the winter-spring transition. The EAGER effort will enable the researchers to conduct the \"omics\" analyses of the phytoplankton to determine predominant means by which energy is acquired and used in these settings. This EAGER effort will apply new expertise to fill an existing gap in ecological observations along the West Antarctic Peninsula. The principle product of the proposed work will be a novel dataset to be analyzed and by an early career researcher from an underserved community (veteran). The critical baseline data contained in this dataset enable a comparison of eukaryotic and prokaryotic gene expression patterns to establish the relative importance of chemoautotrophy, heterotrophy, mixotrophy, and phototrophy during the experiments. this information and data will be made immediately available to the broader scientific community, and will enable the development of further hypotheses on ecosystem change as sea ice cover changes in the region. Very little gene expression data is currently available for the Antarctic marine environment, and no gene expression data is available during the ecologically critical winter to spring transition. Moreover, ice cover in bays is common along the West Antarctic Peninsula yet the opportunity to study cryptophyte phytoplankton physiology beneath such ice conditions in coastal embayments is rare.", "east": -63.9, "geometry": "POINT(-64 -64.875)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "USAP-DC; NOT APPLICABLE; Antarctica; COASTAL", "locations": "Antarctica", "north": -64.75, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Bowman, Jeff", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "NCBI SRA", "repositories": "NCBI SRA; USAP-DC", "science_programs": null, "south": -65.0, "title": "A Preliminary Assessment of the Influence of Ice Cover on Microbial Carbon and Energy Acquisition during the Antarctic Winter-spring Seasonal Transition", "uid": "p0010003", "west": -64.1}, {"awards": "1144177 Pettit, Erin; 1144176 Lyons, W. Berry; 1144192 Tulaczyk, Slawek; 1727387 Mikucki, Jill", "bounds_geometry": "POLYGON((161.8 -77.7,161.88 -77.7,161.96 -77.7,162.04000000000002 -77.7,162.12 -77.7,162.2 -77.7,162.28 -77.7,162.36 -77.7,162.44 -77.7,162.51999999999998 -77.7,162.6 -77.7,162.6 -77.70700000000001,162.6 -77.714,162.6 -77.721,162.6 -77.728,162.6 -77.735,162.6 -77.742,162.6 -77.749,162.6 -77.756,162.6 -77.76299999999999,162.6 -77.77,162.51999999999998 -77.77,162.44 -77.77,162.36 -77.77,162.28 -77.77,162.2 -77.77,162.12 -77.77,162.04000000000002 -77.77,161.96 -77.77,161.88 -77.77,161.8 -77.77,161.8 -77.76299999999999,161.8 -77.756,161.8 -77.749,161.8 -77.742,161.8 -77.735,161.8 -77.728,161.8 -77.721,161.8 -77.714,161.8 -77.70700000000001,161.8 -77.7))", "dataset_titles": "Ablation Stake Data from of Taylor Glacier near Blood Falls; Antarctica Support 2014/2015 - C-528 Blood Falls GPS/GNSS Observations Dataset; Blood Falls, McMurdo Dry Va. International Federation of Digital Seismograph Networks. Dataset/Seismic Network; FLIR thermal imaging data near Blood Falls, Taylor Glacier; Ground Penetrating Radar Data near Blood Falls, Taylor Glacier; Ice Temperature in Shallow Boreholes Near Blood Falls at the Terminus of Taylor Glacier, McMurdo Dry Valleys, Antarctica; NCBI short read archive -Metagenomic survey of Antarctic Groundwater; Terrestrial Radar Interferometry near Blood Falls, Taylor Glacier; The Geochemistry of englacial brine from Taylor Glacier, Antarctica; Time Lapse imagery of the Blood Falls feature, Antarctica ; Vaisala Integrated Met Station near Blood Falls, Taylor Glacier", "datasets": [{"dataset_uid": "601166", "doi": "10.15784/601166", "keywords": "Antarctica; Basal Crevassing; Glacier Hydrology; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; GPR; Radar; Snow/ice; Snow/Ice; Taylor Glacier", "people": "Pettit, Erin", "repository": "USAP-DC", "science_program": null, "title": "Terrestrial Radar Interferometry near Blood Falls, Taylor Glacier", "url": "https://www.usap-dc.org/view/dataset/601166"}, {"dataset_uid": "601167", "doi": "10.15784/601167", "keywords": "Antarctica; Basal Crevassing; Glacier Hydrology; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Photo; Photo/video; Photo/Video; Snow/ice; Snow/Ice; Taylor Glacier; Timelaps Images", "people": "Pettit, Erin", "repository": "USAP-DC", "science_program": null, "title": "Time Lapse imagery of the Blood Falls feature, Antarctica ", "url": "https://www.usap-dc.org/view/dataset/601167"}, {"dataset_uid": "601168", "doi": "10.15784/601168", "keywords": "Antarctica; Atmosphere; Basal Crevassing; Glacier Hydrology; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Meteorology; Taylor Glacier; Temperature; Weather Station Data; Wind Speed", "people": "Pettit, Erin", "repository": "USAP-DC", "science_program": null, "title": "Vaisala Integrated Met Station near Blood Falls, Taylor Glacier", "url": "https://www.usap-dc.org/view/dataset/601168"}, {"dataset_uid": "601169", "doi": "10.15784/601169", "keywords": "Antarctica; Basal Crevassing; Glacier Hydrology; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Infrared Imagery; Photo/video; Photo/Video; Taylor Glacier; Thermal Camera; Timelaps Images", "people": "Pettit, Erin", "repository": "USAP-DC", "science_program": null, "title": "FLIR thermal imaging data near Blood Falls, Taylor Glacier", "url": "https://www.usap-dc.org/view/dataset/601169"}, {"dataset_uid": "601179", "doi": "10.15784/601179", "keywords": "Antarctica; Chemistry:ice; Chemistry:Ice; Geochemistry; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Subglacial Brine", "people": "Lyons, W. Berry; Gardner, Christopher B.", "repository": "USAP-DC", "science_program": null, "title": "The Geochemistry of englacial brine from Taylor Glacier, Antarctica", "url": "https://www.usap-dc.org/view/dataset/601179"}, {"dataset_uid": "601139", "doi": "10.15784/601139", "keywords": "Antarctica; Borehole; Borehole Logging; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Ice Temperature; Snow/ice; Snow/Ice; Temperature; Temperature Profiles", "people": "Tulaczyk, Slawek", "repository": "USAP-DC", "science_program": null, "title": "Ice Temperature in Shallow Boreholes Near Blood Falls at the Terminus of Taylor Glacier, McMurdo Dry Valleys, Antarctica", "url": "https://www.usap-dc.org/view/dataset/601139"}, {"dataset_uid": "200074", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "NCBI short read archive -Metagenomic survey of Antarctic Groundwater", "url": "https://www.ncbi.nlm.nih.gov/sra/?term=SRR6667787"}, {"dataset_uid": "200029", "doi": "10.7914/SN/YW_2013", "keywords": null, "people": null, "repository": "IRIS", "science_program": null, "title": "Blood Falls, McMurdo Dry Va. International Federation of Digital Seismograph Networks. Dataset/Seismic Network", "url": "http://www.fdsn.org/networks/detail/YW_2013/"}, {"dataset_uid": "601164", "doi": "10.15784/601164", "keywords": "Antarctica; Basal Crevassing; Glacier Hydrology; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Snow/ice; Snow/Ice", "people": "Pettit, Erin", "repository": "USAP-DC", "science_program": null, "title": "Ablation Stake Data from of Taylor Glacier near Blood Falls", "url": "https://www.usap-dc.org/view/dataset/601164"}, {"dataset_uid": "601165", "doi": "10.15784/601165", "keywords": "Antarctica; Basal Crevassing; Glacier Hydrology; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; GPR; Radar; Snow/ice; Snow/Ice; Taylor Glacier", "people": "Pettit, Erin", "repository": "USAP-DC", "science_program": null, "title": "Ground Penetrating Radar Data near Blood Falls, Taylor Glacier", "url": "https://www.usap-dc.org/view/dataset/601165"}, {"dataset_uid": "200028", "doi": "10.7283/FCEN-8050", "keywords": null, "people": null, "repository": "UNAVCO", "science_program": null, "title": "Antarctica Support 2014/2015 - C-528 Blood Falls GPS/GNSS Observations Dataset", "url": "https://www.unavco.org/data/doi/10.7283/fcen-8050"}], "date_created": "Wed, 28 Nov 2018 00:00:00 GMT", "description": "Recent discoveries of widespread liquid water and microbial ecosystems below the Antarctic ice sheets have generated considerable interest in studying Antarctic subglacial environments. Understanding subglacial hydrology, the persistence of life in extended isolation and the evolution and stability of subglacial habitats requires an integrated, interdisciplinary approach. The collaborative project, Minimally Invasive Direct Glacial Exploration (MIDGE) of the Biogeochemistry, Hydrology and Glaciology of Blood Falls, McMurdo Dry Valleys will integrate geophysical measurements, molecular microbial ecology and geochemical analyses to explore a unique Antarctic subglacial system known as Blood Falls. Blood Falls is a hypersaline, subglacial brine that supports an active microbial community. The subglacial brine is released from a crevasse at the surface of the Taylor Glacier providing an accessible portal into an Antarctic subglacial ecosystem. Recent geochemical and molecular analyses support a marine source for the salts and microorganisms in Blood Falls. The last time marine waters inundated this part of the McMurdo Dry Valleys was during the Late Tertiary, which suggests the brine is ancient. Still, no direct samples have been collected from the subglacial source to Blood Falls and little is known about the origin of this brine or the amount of time it has been sealed below Taylor Glacier. Radar profiles collected near Blood Falls delineate a possible fault in the subglacial substrate that may help explain the localized and episodic nature of brine release. However it remains unclear what triggers the episodic release of brine exclusively at the Blood Falls crevasse or the extent to which the brine is altered as it makes its way to the surface. The MIDGE project aims to determine the mechanism of brine release at Blood Falls, evaluate changes in the geochemistry and the microbial community within the englacial conduit and assess if Blood Falls waters have a distinct impact on the thermal and stress state of Taylor Glacier, one of the most studied polar glaciers in Antarctica. The geophysical study of the glaciological structure and mechanism of brine release will use GPR, GPS, and a small passive seismic network. Together with international collaborators, the \u0027Ice Mole\u0027 team from FH Aachen University of Applied Sciences, Germany (funded by the German Aerospace Center, DLR), MIDGE will develop and deploy innovative, minimally invasive technologies for clean access and brine sample retrieval from deep within the Blood Falls drainage system. These technologies will allow for the collection of samples of the brine away from the surface (up to tens of meters) for geochemical analyses and microbial structure-function experiments. There is concern over the contamination of pristine subglacial environments from chemical and biological materials inherent in the drilling process; and MIDGE will provide data on the efficacy of thermoelectric probes for clean access and retrieval of representative subglacial samples. Antarctic subglacial environments provide an excellent opportunity for researching survivability and adaptability of microbial life and are potential terrestrial analogues for life habitats on icy planetary bodies. The MIDGE project offers a portable, versatile, clean alternative to hot water and mechanical drilling and will enable the exploration of subglacial hydrology and ecosystem function while making significant progress towards developing technologies for minimally invasive and clean sampling of icy systems.", "east": 162.6, "geometry": "POINT(162.2 -77.735)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "NOT APPLICABLE; BACTERIA/ARCHAEA; USAP-DC", "locations": null, "north": -77.7, "nsf_funding_programs": "Antarctic Integrated System Science; Antarctic Integrated System Science; Antarctic Integrated System Science; Antarctic Integrated System Science; Antarctic Instrumentation and Support", "paleo_time": null, "persons": "Tulaczyk, Slawek; Pettit, Erin; Lyons, W. Berry; Mikucki, Jill", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "USAP-DC", "repositories": "IRIS; NCBI GenBank; UNAVCO; USAP-DC", "science_programs": null, "south": -77.77, "title": "Collaborative Research: MIDGE: Minimally Invasive Direct Glacial Exploration of Biogeochemistry, Hydrology and Glaciology of Blood Falls, McMurdo Dry Valleys", "uid": "p0000002", "west": 161.8}, {"awards": "0839142 Tulaczyk, Slawek; 0839059 Powell, Ross; 0838764 Anandakrishnan, Sridhar; 0838947 Tulaczyk, Slawek; 0838855 Jacobel, Robert; 0838763 Anandakrishnan, Sridhar; 0839107 Powell, Ross", "bounds_geometry": null, "dataset_titles": "Basal melt rates of the Ross Ice Shelf near the Whillans Ice Stream grounding line; Integrative Study of Marine Ice Sheet Stability and Subglacial Life Habitats in W Antarctica - Lake and Ice Stream Subglacial Access Research Drilling (LISSARD); Integrative Study of Marine Ice Sheet Stability and Subglacial Life Habitats - Robotic Access to Grounding-zones for Exploration and Science (RAGES); IRIS ID#s 201035, 201162, 201205; IRIS offers free and open access to a comprehensive data store of raw geophysical time-series data collected from a large variety of sensors, courtesy of a vast array of US and International scientific networks, including seismometers (permanent and temporary), tilt and strain meters, infrasound, temperature, atmospheric pressure and gravimeters, to support basic research aimed at imaging the Earth\u0027s interior.; Paleogene marine and terrestrial development of the West Antarctic Rift System: Biomarker Data Set; Paleogene marine and terrestrial development of the West Antarctic Rift System: Palynomorph Data Set; Radar Studies of Subglacial Lake Whillans and the Whillans Ice Stream Grounding Zone; The IRIS DMC archives and distributes data to support the seismological research community.; UNAVCO ID#s WHL1, WHL2, LA02, LA09 (full data link not provided)", "datasets": [{"dataset_uid": "609594", "doi": "10.7265/N54J0C2W", "keywords": "Antarctica; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; GPR; GPS; Radar; Whillans Ice Stream", "people": "Jacobel, Robert", "repository": "USAP-DC", "science_program": null, "title": "Radar Studies of Subglacial Lake Whillans and the Whillans Ice Stream Grounding Zone", "url": "https://www.usap-dc.org/view/dataset/609594"}, {"dataset_uid": "001406", "doi": "", "keywords": null, "people": null, "repository": "IRIS", "science_program": null, "title": "The IRIS DMC archives and distributes data to support the seismological research community.", "url": "http://ds.iris.edu/ds/nodes/dmc/"}, {"dataset_uid": "001405", "doi": "", "keywords": null, "people": null, "repository": "IRIS", "science_program": null, "title": "IRIS offers free and open access to a comprehensive data store of raw geophysical time-series data collected from a large variety of sensors, courtesy of a vast array of US and International scientific networks, including seismometers (permanent and temporary), tilt and strain meters, infrasound, temperature, atmospheric pressure and gravimeters, to support basic research aimed at imaging the Earth\u0027s interior.", "url": "http://www.iris.edu/hq/data_and_software"}, {"dataset_uid": "601234", "doi": "10.15784/601234", "keywords": "ACL; Antarctica; Biomarker; BIT Index; Glaciers/ice Sheet; Glaciers/Ice Sheet; Ice Stream; Whillans Ice Stream; WISSARD", "people": "Scherer, Reed Paul; Baudoin, Patrick; Warny, Sophie; Coenen, Jason; Askin, Rosemary; Casta\u00f1eda, Isla", "repository": "USAP-DC", "science_program": "WISSARD", "title": "Paleogene marine and terrestrial development of the West Antarctic Rift System: Biomarker Data Set", "url": "https://www.usap-dc.org/view/dataset/601234"}, {"dataset_uid": "601245", "doi": "10.15784/601245", "keywords": "Antarctica; Pollen; West Antarctica; WISSARD", "people": "Baudoin, Patrick; Coenen, Jason; Warny, Sophie; Askin, Rosemary; Scherer, Reed Paul; Casta\u00f1eda, Isla", "repository": "USAP-DC", "science_program": "WISSARD", "title": "Paleogene marine and terrestrial development of the West Antarctic Rift System: Palynomorph Data Set", "url": "https://www.usap-dc.org/view/dataset/601245"}, {"dataset_uid": "601122", "doi": "10.15784/601122", "keywords": "Antarctica; Flexure Zone; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Ice Shelf; Ice-Shelf Basal Melting; Ice-Shelf Strain Rate", "people": "Begeman, Carolyn", "repository": "USAP-DC", "science_program": "WISSARD", "title": "Basal melt rates of the Ross Ice Shelf near the Whillans Ice Stream grounding line", "url": "https://www.usap-dc.org/view/dataset/601122"}, {"dataset_uid": "600155", "doi": "10.15784/600155", "keywords": "Antarctica; Glaciology; Oceans; Southern Ocean; WISSARD", "people": "Powell, Ross", "repository": "USAP-DC", "science_program": null, "title": "Integrative Study of Marine Ice Sheet Stability and Subglacial Life Habitats - Robotic Access to Grounding-zones for Exploration and Science (RAGES)", "url": "https://www.usap-dc.org/view/dataset/600155"}, {"dataset_uid": "600154", "doi": "10.15784/600154", "keywords": "Antarctica; Biota; Diatom; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Ice Core Records; Lake Whillans; Paleoclimate; Ross Sea; Southern Ocean; Subglacial Lake; WISSARD", "people": "Powell, Ross", "repository": "USAP-DC", "science_program": null, "title": "Integrative Study of Marine Ice Sheet Stability and Subglacial Life Habitats in W Antarctica - Lake and Ice Stream Subglacial Access Research Drilling (LISSARD)", "url": "https://www.usap-dc.org/view/dataset/600154"}, {"dataset_uid": "000150", "doi": "", "keywords": null, "people": null, "repository": "UNAVCO", "science_program": null, "title": "UNAVCO ID#s WHL1, WHL2, LA02, LA09 (full data link not provided)", "url": "http://www.unavco.org/"}, {"dataset_uid": "000148", "doi": "", "keywords": null, "people": null, "repository": "IRIS", "science_program": null, "title": "IRIS ID#s 201035, 201162, 201205", "url": "http://ds.iris.edu/"}], "date_created": "Mon, 10 Sep 2018 00:00:00 GMT", "description": "This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5). The LISSARD project (Lake and Ice Stream Subglacial Access Research Drilling) is one of three research components of the WISSARD integrative initiative (Whillans Ice Stream Subglacial Access Research Drilling) that is being funded by the Antarctic Integrated System Science Program of NSF\u0027s Office of Polar Programs, Antarctic Division. The overarching scientific objective of WISSARD is to assess the role of water beneath a West Antarctic ice stream in interlinked glaciological, geological, microbiological, geochemical, and oceanographic systems. The LISSARD component of WISSARD focuses on the role of active subglacial lakes in determining how fast the West Antarctic ice sheet loses mass to the global ocean and influences global sea level changes. The importance of Antarctic subglacial lakes has only been recently recognized, and the lakes have been identified as high priority targets for scientific investigations because of their unknown contributions to ice sheet stability under future global warming scenarios. LISSARD has several primary science goals: A) To provide an observational basis for improving treatments of subglacial hydrological and mechanical processes in models of ice sheet mass balance and stability; B) To reconstruct the past history of ice stream stability by analyzing archives of past basal water and ice flow variability contained in subglacial sediments, porewater, lake water, and basal accreted ice; C) To provide background understanding of subglacial lake environments to benefit RAGES and GBASE (the other two components of the WISSARD project); and D) To synthesize data and concepts developed as part of this project to determine whether subglacial lakes play an important role in (de)stabilizing Antarctic ice sheets. We propose an unprecedented synthesis of approaches to studying ice sheet processes, including: (1) satellite remote sensing, (2) surface geophysics, (3) borehole observations and measurements and, (4) basal and subglacial sampling. \u003cbr/\u003e\u003cbr/\u003eINTELLECTUAL MERIT: The latest report of the Intergovernmental Panel on Climate Change recognized that the greatest uncertainties in assessing future global sea-level change stem from a poor understanding of ice sheet dynamics and ice sheet vulnerability to oceanic and atmospheric warming. Disintegration of the WAIS (West Antarctic Ice Sheet) alone would contribute 3-5 m to global sea-level rise, making WAIS a focus of scientific concern due to its potential susceptibility to internal or ocean-driven instability. The overall WISSARD project will test the overarching hypothesis that active water drainage connects various subglacial environments and exerts major control on ice sheet flow, geochemistry, metabolic and phylogenetic diversity, and biogeochemical transformations. \u003cbr/\u003e\u003cbr/\u003eBROADER IMPACTS: Societal Relevance: Global warming, melting of ice sheets and consequential sea-level rise are of high societal relevance. Science Resource Development: After a 9-year hiatus WISSARD will provide the US-science community with a renewed capability to access and study sub-ice sheet environments. Developing this technological infrastructure will benefit the broader science community and assets will be accessible for future use through the NSF-OPP drilling contractor. Furthermore, these projects will pioneer an approach implementing recommendations from the National Research Council committee on Principles of Environmental Stewardship for the Exploration and Study of Subglacial Environments (2007). Education and Outreach (E/O): These activities are grouped into four categories: i) increasing student participation in polar research by fully integrating them in our research programs; ii) introducing new investigators to the polar sciences by incorporating promising young investigators in our programs, iii) promotion of K-12 teaching and learning programs by incorporating various teachers and NSTA programs, and iv) reaching a larger public audience through such venues as popular science magazines, museum based activities and videography and documentary films. In summary, WISSARD will promote scientific exploration of Antarctica by conveying to the public the excitement of accessing and studying what may be some of the last unexplored aquatic environments on Earth, and which represent a potential analogue for extraterrestrial life habitats on Europa and Mars.", "east": null, "geometry": null, "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE; IN SITU/LABORATORY INSTRUMENTS \u003e CORERS \u003e CORING DEVICES; EARTH REMOTE SENSING INSTRUMENTS \u003e ACTIVE REMOTE SENSING \u003e PROFILERS/SOUNDERS \u003e RADAR SOUNDERS \u003e RADAR; IN SITU/LABORATORY INSTRUMENTS \u003e MAGNETIC/MOTION SENSORS \u003e SEISMOMETERS \u003e SEISMOGRAPHS; IN SITU/LABORATORY INSTRUMENTS \u003e MAGNETIC/MOTION SENSORS \u003e SEISMOMETERS \u003e SEISMOMETERS", "is_usap_dc": true, "keywords": "USAP-DC; Ice Penetrating Radar; Antarctic; Subglacial Lake; Subglacial Hydrology; Grounding Line; Sea Level Rise; Bed Reflectivity; Ice Sheet Stability; Stability; Radar; Sub-Ice-Shelf; Geophysics; Biogeochemical; LABORATORY; Sediment; Sea Floor Sediment; Ice Thickness; Model; Ice Stream Stability; Basal Ice; SATELLITES; Ice Sheet Thickness; Subglacial; Antarctica; NOT APPLICABLE; Antarctic Ice Sheet; Ice Sheet; FIELD SURVEYS; Surface Elevation; Geochemistry; FIELD INVESTIGATION; Not provided", "locations": "Antarctic; Antarctica; Antarctic Ice Sheet", "north": null, "nsf_funding_programs": "Antarctic Integrated System Science; Antarctic Integrated System Science; Antarctic Integrated System Science; Antarctic Integrated System Science; Antarctic Integrated System Science; Antarctic Integrated System Science; Antarctic Integrated System Science", "paleo_time": null, "persons": "Tulaczyk, Slawek; Fisher, Andrew; Powell, Ross; Anandakrishnan, Sridhar; Jacobel, Robert; Scherer, Reed Paul", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION; LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS; Not provided; OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE; OTHER \u003e PHYSICAL MODELS \u003e LABORATORY; SPACE-BASED PLATFORMS \u003e EARTH OBSERVATION SATELLITES \u003e SATELLITES", "repo": "USAP-DC", "repositories": "IRIS; UNAVCO; USAP-DC", "science_programs": "WISSARD", "south": null, "title": "Collaborative Research: Integrative Study of Marine Ice Sheet Stability \u0026 Subglacial Life Habitats in W Antarctica - Lake \u0026 Ice Stream Subglacial Access Research Drilling (LISSARD)", "uid": "p0000105", "west": null}, {"awards": "1246292 Cary, Stephen", "bounds_geometry": "POLYGON((161.36062 -77.20215,161.610171 -77.20215,161.859722 -77.20215,162.109273 -77.20215,162.358824 -77.20215,162.608375 -77.20215,162.857926 -77.20215,163.107477 -77.20215,163.357028 -77.20215,163.606579 -77.20215,163.85613 -77.20215,163.85613 -77.291278,163.85613 -77.380406,163.85613 -77.469534,163.85613 -77.558662,163.85613 -77.64779,163.85613 -77.736918,163.85613 -77.826046,163.85613 -77.915174,163.85613 -78.004302,163.85613 -78.09343,163.606579 -78.09343,163.357028 -78.09343,163.107477 -78.09343,162.857926 -78.09343,162.608375 -78.09343,162.358824 -78.09343,162.109273 -78.09343,161.859722 -78.09343,161.610171 -78.09343,161.36062 -78.09343,161.36062 -78.004302,161.36062 -77.915174,161.36062 -77.826046,161.36062 -77.736918,161.36062 -77.64779,161.36062 -77.558662,161.36062 -77.469534,161.36062 -77.380406,161.36062 -77.291278,161.36062 -77.20215))", "dataset_titles": "Carbon-fixation rates and associated microbial communities; Carbon-fixation rates and associated microbial communities residing in arid and ephemerally wet Antarctic Dry Valley soils; Importance of Heterotrophic and Phototrophic N2 Fixation in the McMurdo Dry Valleys ; Microbial community composition of transiently wetted Antarctic Dry Valley soils.; Microbial population dynamics along a terrestrial Antarctic moisture gradient; Microbial population dynamics along a terrestrial wetted gradient", "datasets": [{"dataset_uid": "200015", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Microbial community composition of transiently wetted Antarctic Dry Valley soils.", "url": "https://www.ncbi.nlm.nih.gov/popset/?term=KP836071%20to%20KP836108"}, {"dataset_uid": "002738", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Carbon-fixation rates and associated microbial communities", "url": "https://www.ncbi.nlm.nih.gov/protein/?term=craig%20cary"}, {"dataset_uid": "200014", "doi": "", "keywords": null, "people": null, "repository": "EMBL", "science_program": null, "title": "Microbial population dynamics along a terrestrial wetted gradient", "url": "https://www.ebi.ac.uk/ena/data/view/PRJEB7939"}, {"dataset_uid": "002737", "doi": "", "keywords": null, "people": null, "repository": "KNB", "science_program": null, "title": "Carbon-fixation rates and associated microbial communities residing in arid and ephemerally wet Antarctic Dry Valley soils", "url": "https://knb.ecoinformatics.org/view/knb.756.1"}, {"dataset_uid": "002736", "doi": "", "keywords": null, "people": null, "repository": "EMBL", "science_program": null, "title": "Microbial population dynamics along a terrestrial Antarctic moisture gradient", "url": "https://www.ebi.ac.uk/ena/data/view/PRJEB27415"}, {"dataset_uid": "200013", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Importance of Heterotrophic and Phototrophic N2 Fixation in the McMurdo Dry Valleys ", "url": "https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA505820"}], "date_created": "Wed, 14 Mar 2018 00:00:00 GMT", "description": "The McMurdo Dry Valleys in Antarctica are recognized as being the driest, coldest and probably one of the harshest environments on Earth. In addition to the lack of water, the biota in the valleys face a very limited supply of nutrients such as nitrogen compounds - necessary for protein synthesis. The glacial streams of the Dry Valleys have extensive cyanobacterial (blue green algae) mats that are a major source of carbon and nitrogen compounds to biota in this region. While cyanobacteria in streams are important as a source of these compounds, other non-photosynthetic bacteria also contribute a significant fraction (~50%) of fixed nitrogen compounds to valley biota. This research effort will involve an examination of exactly which non-phototrophic bacteria are involved in nitrogen fixation and what environmental factors are responsible for controlling nitrogen fixation by these microbes. This work will resolve the environmental factors that control the activity, abundance and diversity of nitrogen-fixing microbes across four of the McMurdo Dry Valleys. This will allow for comparisons among sites of differing latitude, temperature, elevation and exposure to water. These results will be integrated into a landscape wetness model that will help determine the impact of both cyanobacterial and non-photosynthetic nitrogen fixing microorganisms in this very harsh environment. The Dry Valleys in many ways resemble the Martian environment, and understanding the primitive life and very simple nutrient cycling in the Dry Valleys has relevance for understanding how life might have once existed on other planets. Furthermore, the study of microbes from extreme environments has resulted in numerous biotechnological applications such as the polymerase chain reaction for amplifying DNA and mechanisms for freeze resistance in agricultural crops. Thus, this research should yield insights into how biota survive in extreme environments, and these insights could lead to other commercial applications.", "east": 163.85613, "geometry": "POINT(162.608375 -77.64779)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "NOT APPLICABLE; Antarctica; USAP-DC; RIVERS/STREAM", "locations": "Antarctica", "north": -77.20215, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Cary, Stephen", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "NCBI GenBank", "repositories": "EMBL; KNB; NCBI GenBank", "science_programs": null, "south": -78.09343, "title": "Collaborative Research: Importance of Heterotrophic and Phototrophic N2 Fixation in the McMurdo Dry Valleys on Local, Regional and Landscape Scales", "uid": "p0000235", "west": 161.36062}, {"awards": "1056396 Morgan-Kiss, Rachael", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "Genetic sequence identifier: Accession Numbers: GU132860-GU132939; JN091926-JN091960; JQ9243533-JQ924384; KJ848331-KJ848439; KU196097-KU196166; PRJNA396917", "datasets": [{"dataset_uid": "000241", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Genetic sequence identifier: Accession Numbers: GU132860-GU132939; JN091926-JN091960; JQ9243533-JQ924384; KJ848331-KJ848439; KU196097-KU196166; PRJNA396917", "url": "https://www.ncbi.nlm.nih.gov/genbank/"}], "date_created": "Mon, 26 Feb 2018 00:00:00 GMT", "description": "This CAREER proposal will support an early career female PI to establish an integrated research and education program in the fields of polar biology and environmental microbiology, focusing on single-celled eukaryotes (protists) in high latitude ice-covered Antarctic lakes systems. Protists play important roles in energy flow and material cycling, and act as both primary producers (fixing inorganic carbon by photosynthesis) and consumers (preying on bacteria by phagotrophic digestion). The McMurdo Dry Valleys (MDV) located in Victoria Land, Antarctica, harbor microbial communities which are isolated in the unique aquatic ecosystem of perennially ice-capped lakes. The lakes support exclusively microbial consortia in chemically stratified water columns that are not influenced by seasonal mixing, allochthonous inputs, or direct human impact. This project will exploit permanently stratified biogeochemistry that is unique across the water columns of several MDV lakes to address gaps in our understanding of protist trophic function in aquatic food webs. The proposed research will examine (1) the impact of permanent biogeochemical gradients on protist trophic strategy, (2) the effect of major abiotic drivers (light and nutrients) on the distribution of two key mixotrophic and photoautotrophic protist species, and (3) the effect of episodic nutrient pulses on mixotroph communities in high latitude (ultraoligotrophic) MDV lakes versus low latitude (eutrophic) watersheds. The project will impact the fields of microbial ecology and environmental microbiology by combining results from field, laboratory and in situ incubation studies to synthesize new models for the protist trophic roles in the aquatic food web. The research component of this proposed project will be tightly integrated with the development of two new education activities designed to exploit the inherent excitement associated with polar biological research. The educational objectives are: 1) to establish a teaching module in polar biology in a core undergraduate course for microbiology majors; 2) to develop an instructional module to engage middle school girls in STEM disciplines. Undergraduates and middle school girls will also work with a doctoral student on his experiments in local Ohio watersheds.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "NOT APPLICABLE; USAP-DC", "locations": null, "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Morgan-Kiss, Rachael", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "NCBI GenBank", "repositories": "NCBI GenBank", "science_programs": null, "south": -90.0, "title": "CAREER:Protist Nutritional Strategies in Permanently Stratified Antarctic Lakes", "uid": "p0000310", "west": -180.0}, {"awards": "0732711 Smith, Craig; 0732625 Leventer, Amy; 0732655 Mosley-Thompson, Ellen; 0732602 Truffer, Martin; 0732651 Gordon, Arnold; 0732983 Vernet, Maria", "bounds_geometry": "POLYGON((-68 -57.8,-66.78 -57.8,-65.56 -57.8,-64.34 -57.8,-63.12 -57.8,-61.9 -57.8,-60.68 -57.8,-59.46 -57.8,-58.24 -57.8,-57.02 -57.8,-55.8 -57.8,-55.8 -58.8,-55.8 -59.8,-55.8 -60.8,-55.8 -61.8,-55.8 -62.8,-55.8 -63.8,-55.8 -64.8,-55.8 -65.8,-55.8 -66.8,-55.8 -67.8,-57.02 -67.8,-58.24 -67.8,-59.46 -67.8,-60.68 -67.8,-61.9 -67.8,-63.12 -67.8,-64.34 -67.8,-65.56 -67.8,-66.78 -67.8,-68 -67.8,-68 -66.8,-68 -65.8,-68 -64.8,-68 -63.8,-68 -62.8,-68 -61.8,-68 -60.8,-68 -59.8,-68 -58.8,-68 -57.8))", "dataset_titles": "Abrupt Environmental Change in the Larsen Ice Shelf System (LARISSA) - Marine Ecosystems; Biology Species Abundance from the Larsen Ice Shelf acquired during the Nathaniel B. Palmer expeditions NBP1001 and NBP1203; Bruce Plateau Accumulation O18 2009-1900; Easten Antarctic Peninsula Surface Sediment Diatom Data; LMG13-11 JKC-1 Paleoceanographic data; Macrofauna Species Abundance Raw Data from the Larsen Ice Shelf acquired during the Nathaniel B. Palmer expedition NBP1001; Megafauna Species Abundance Raw Data from the Larsen Ice Shelf acquired during the Nathaniel B. Palmer expedition NBP1001; NBP1001 cruise data; NBP1203 cruise data; Processed CTD Data from the Larsen Ice Shelf in Antarctica acquired during the Nathaniel B. Palmer expedition NBP1001; Processed CTD Data from the Larsen Ice Shelf near Antarctica acquired during the Nathaniel B. Palmer expedition NBP1203; Processed ship-based LADCP Sonar Data from the Larsen Ice Shelf in Antarctica acquired during the Nathaniel B. Palmer expedition NBP1001; Processed ship-based LADCP Sonar Data from the Larsen Ice Shelf near Antarctica acquired during the Nathaniel B. Palmer expedition NBP1203; Radioisotope data (C-14 and Pb-210) from bulk sediments, Larsen A Ice Shelf; Sediment samples (full data link not provided)", "datasets": [{"dataset_uid": "601306", "doi": null, "keywords": "Antarctica; Antarctic Peninsula; Biota; Box Corer; LARISSA; Larsen Ice Shelf; Macrofauna; NBP1001; Oceans; R/v Nathaniel B. Palmer; Seafloor Sampling; Species Abundance", "people": "Smith, Craig", "repository": "USAP-DC", "science_program": "LARISSA", "title": "Macrofauna Species Abundance Raw Data from the Larsen Ice Shelf acquired during the Nathaniel B. Palmer expedition NBP1001", "url": "https://www.usap-dc.org/view/dataset/601306"}, {"dataset_uid": "601348", "doi": null, "keywords": "Antarctica; Antarctic Peninsula; CTD; CTD Data; LARISSA; Larsen Ice Shelf; NBP1203; Oceans; Physical Oceanography; R/v Nathaniel B. Palmer; Salinity; Temperature", "people": "Huber, Bruce; Gordon, Arnold", "repository": "USAP-DC", "science_program": null, "title": "Processed CTD Data from the Larsen Ice Shelf near Antarctica acquired during the Nathaniel B. Palmer expedition NBP1203", "url": "https://www.usap-dc.org/view/dataset/601348"}, {"dataset_uid": "000226", "doi": "", "keywords": null, "people": null, "repository": "MGDS", "science_program": null, "title": "Biology Species Abundance from the Larsen Ice Shelf acquired during the Nathaniel B. Palmer expeditions NBP1001 and NBP1203", "url": "https://doi.org/10.1594/ieda/320821"}, {"dataset_uid": "600073", "doi": "10.15784/600073", "keywords": "Antarctica; Antarctic Peninsula; Araon1304; Biota; LARISSA; Larsen B Ice Shelf; NBP1001; NBP1203; Oceans; Physical Oceanography; Southern Ocean; Weddell Sea", "people": "Vernet, Maria", "repository": "USAP-DC", "science_program": "LARISSA", "title": "Abrupt Environmental Change in the Larsen Ice Shelf System (LARISSA) - Marine Ecosystems", "url": "https://www.usap-dc.org/view/dataset/600073"}, {"dataset_uid": "600167", "doi": "10.15784/600167", "keywords": "Antarctica; Antarctic Peninsula; Bruce Plateau; Glaciology; Ice Core Records; Isotope; LARISSA; Paleoclimate; Sample/collection Description; Sample/Collection Description; Snow Accumulation", "people": "Thompson, Lonnie G.; Mosley-Thompson, Ellen", "repository": "USAP-DC", "science_program": "LARISSA", "title": "Bruce Plateau Accumulation O18 2009-1900", "url": "https://www.usap-dc.org/view/dataset/600167"}, {"dataset_uid": "601346", "doi": null, "keywords": "Antarctica; Antarctic Peninsula; Current Measurements; LADCP; Larsen Ice Shelf; NBP1001; Oceans; Physical Oceanography; R/v Nathaniel B. Palmer", "people": "Huber, Bruce; Gordon, Arnold", "repository": "USAP-DC", "science_program": "LARISSA", "title": "Processed ship-based LADCP Sonar Data from the Larsen Ice Shelf in Antarctica acquired during the Nathaniel B. Palmer expedition NBP1001", "url": "https://www.usap-dc.org/view/dataset/601346"}, {"dataset_uid": "601336", "doi": "10.15784/601336", "keywords": "Antarctica; Carbon-14; Larsen Ice Shelf; Lead-210; Marine Sediments; Radioisotope Analysis", "people": "Taylor, Richard; DeMaster, David", "repository": "USAP-DC", "science_program": "LARISSA", "title": "Radioisotope data (C-14 and Pb-210) from bulk sediments, Larsen A Ice Shelf", "url": "https://www.usap-dc.org/view/dataset/601336"}, {"dataset_uid": "601211", "doi": "10.15784/601211", "keywords": "Antarctica; Antarctic Peninsula; Benthos; Biota; Diatom; Geology/Geophysics - Other; LMG0502; Marine Geoscience; Marine Sediments; Microscope; NBP0003; NBP0107; NBP0603; NBP1203; R/v Nathaniel B. Palmer; Surface Sediment", "people": "Leventer, Amy", "repository": "USAP-DC", "science_program": "LARISSA", "title": "Easten Antarctic Peninsula Surface Sediment Diatom Data", "url": "https://www.usap-dc.org/view/dataset/601211"}, {"dataset_uid": "000145", "doi": "", "keywords": null, "people": null, "repository": "AMGRF", "science_program": null, "title": "Sediment samples (full data link not provided)", "url": "http://arf.fsu.edu/"}, {"dataset_uid": "000142", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "NBP1001 cruise data", "url": "https://www.rvdata.us/search/cruise/NBP1001"}, {"dataset_uid": "601485", "doi": "10.15784/601485", "keywords": "Antarctica; Antarctic Peninsula; Delta 13C; Delta 18O; Paleoceanography; Temperature", "people": "Shevenell, Amelia", "repository": "USAP-DC", "science_program": "LARISSA", "title": "LMG13-11 JKC-1 Paleoceanographic data", "url": "https://www.usap-dc.org/view/dataset/601485"}, {"dataset_uid": "000143", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "NBP1203 cruise data", "url": "https://www.rvdata.us/search/cruise/NBP1203"}, {"dataset_uid": "601347", "doi": null, "keywords": "Antarctica; Antarctic Peninsula; Current Measurements; LADCP; LARISSA; Larsen Ice Shelf; NBP1203; Oceans; Physical Oceanography; R/v Nathaniel B. Palmer", "people": "Gordon, Arnold; Huber, Bruce", "repository": "USAP-DC", "science_program": null, "title": "Processed ship-based LADCP Sonar Data from the Larsen Ice Shelf near Antarctica acquired during the Nathaniel B. Palmer expedition NBP1203", "url": "https://www.usap-dc.org/view/dataset/601347"}, {"dataset_uid": "601305", "doi": null, "keywords": "Antarctica; Antarctic Peninsula; Biota; Box Corer; LARISSA; Larsen Ice Shelf; Macrofauna; Megafauna; NBP1001; Oceans; R/v Nathaniel B. Palmer; Seafloor Sampling; Species Abundance", "people": "Smith, Craig", "repository": "USAP-DC", "science_program": "LARISSA", "title": "Megafauna Species Abundance Raw Data from the Larsen Ice Shelf acquired during the Nathaniel B. Palmer expedition NBP1001", "url": "https://www.usap-dc.org/view/dataset/601305"}, {"dataset_uid": "601345", "doi": null, "keywords": "Antarctica; Antarctic Peninsula; CTD; CTD Data; LARISSA; Larsen Ice Shelf; NBP1001; Oceans; Physical Oceanography; R/v Nathaniel B. Palmer; Salinity; Temperature", "people": "Huber, Bruce; Gordon, Arnold", "repository": "USAP-DC", "science_program": "LARISSA", "title": "Processed CTD Data from the Larsen Ice Shelf in Antarctica acquired during the Nathaniel B. Palmer expedition NBP1001", "url": "https://www.usap-dc.org/view/dataset/601345"}], "date_created": "Thu, 01 Feb 2018 00:00:00 GMT", "description": "Like no other region on Earth, the northern Antarctic Peninsula represents a spectacular natural laboratory of climate change and provides the opportunity to study the record of past climate and ecological shifts alongside the present-day changes in one of the most rapidly warming regions on Earth. This award supports the cryospheric and oceano-graphic components of an integrated multi-disciplinary program to address these rapid and fundamental changes now taking place in Antarctic Peninsula (AP). By making use of a marine research platform (the RV NB Palmer and on-board helicopters) and additional logistical support from the Argentine Antarctic program, the project will bring glaciologists, oceanographers, marine geologists and biologists together, working collaboratively to address fundamentally interdisciplinary questions regarding climate change. The project will include gathering a new, high-resolution paleoclimate record from the Bruce Plateau of Graham Land, and using it to compare Holocene- and possibly glacial-epoch climate to the modern period; investigating the stability of the remaining Larsen Ice Shelf and rapid post-breakup glacier response ? in particular, the roles of surface melt and ice-ocean interactions in the speed-up and retreat; observing the contribution of, and response of, oceanographic systems to ice shelf disintegration and ice-glacier interactions. Helicopter support on board will allow access to a wide range of glacial and geological areas of interest adjacent to the Larsen embayment. At these locations, long-term in situ glacial monitoring, isostatic uplift, and ice flow GPS sites will be established, and high-resolution ice core records will be obtained using previously tested lightweight drilling equipment. Long-term monitoring of deep water outflow will, for the first time, be integrated into changes in ice shelf extent and thickness, bottom water formation, and multi-level circulation by linking near-source observations to distal sites of concentrated outflow. The broader impacts of this international, multidisciplinary effort are that it will significantly advance our understanding of linkages amongst the earth\u0027s systems in the Polar Regions, and are proposed with international participation (UK, Spain, Belgium, Germany and Argentina) and interdisciplinary engagement in the true spirit of the International Polar Year (IPY). It will also provide a means of engaging and educating the public in virtually all aspects of polar science and the effects of ongoing climate change. The research team has a long record of involving undergraduates in research, educating high-performing graduate students, and providing innovative and engaging outreach products to the K-12 education and public media forums. Moreover, forging the new links both in science and international Antarctic programs will provide a continuing legacy, beyond IPY, of improved understanding and cooperation in Antarctica.", "east": -55.8, "geometry": "POINT(-61.9 -62.8)", "instruments": "EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE; IN SITU/LABORATORY INSTRUMENTS \u003e CORERS \u003e BOX CORE; IN SITU/LABORATORY INSTRUMENTS \u003e CORERS \u003e CORING DEVICES; IN SITU/LABORATORY INSTRUMENTS \u003e CORERS \u003e ICE AUGERS; IN SITU/LABORATORY INSTRUMENTS \u003e CORERS \u003e SNOW DENSITY CUTTER", "is_usap_dc": true, "keywords": "Paleoclimate; Megafauna; USAP-DC; AMD; Amd/Us; Antarctica; Climate Change; LABORATORY; Climate Variability; Multi-Disciplinary; Cryosphere; NBP1001; FIELD SURVEYS; Not provided; Antarctic Peninsula; R/V NBP; FIELD INVESTIGATION; USA/NSF; Ice Core; Holocene", "locations": "Antarctica; Antarctic Peninsula", "north": -57.8, "nsf_funding_programs": "Antarctic Integrated System Science; Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems; Antarctic Integrated System Science; Antarctic Integrated System Science; Antarctic Integrated System Science; Antarctic Integrated System Science; Antarctic Integrated System Science", "paleo_time": "PHANEROZOIC \u003e CENOZOIC \u003e QUATERNARY \u003e HOLOCENE", "persons": "Truffer, Martin; Gordon, Arnold; Huber, Bruce; Mosley-Thompson, Ellen; Leventer, Amy; Vernet, Maria; Smith, Craig; Thompson, Lonnie G.", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION; LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS; Not provided; OTHER \u003e PHYSICAL MODELS \u003e LABORATORY; WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repo": "USAP-DC", "repositories": "AMGRF; MGDS; R2R; USAP-DC", "science_programs": "LARISSA", "south": -67.8, "title": "Collaborative Research in IPY: Abrupt Environmental Change in the Larsen Ice Shelf System, a Multidisciplinary Approach -- Cryosphere and Oceans", "uid": "p0000101", "west": -68.0}, {"awards": "1543245 Rynearson, Tatiana", "bounds_geometry": null, "dataset_titles": "Expedition Data; Expedition data of NBP1701; NCBI Popset of 43 Southern Ocean diatom isolates, including accessions ON678208.1 - ON678250.1; Specific growth rate measurements for 43 Southern Ocean diatoms", "datasets": [{"dataset_uid": "002661", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of NBP1701", "url": "https://www.rvdata.us/search/cruise/NBP1701"}, {"dataset_uid": "001369", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/NBP1701"}, {"dataset_uid": "200328", "doi": "", "keywords": null, "people": null, "repository": "NCBI", "science_program": null, "title": "NCBI Popset of 43 Southern Ocean diatom isolates, including accessions ON678208.1 - ON678250.1", "url": "https://www.ncbi.nlm.nih.gov/popset/?term=2248543458"}, {"dataset_uid": "601586", "doi": "10.15784/601586", "keywords": "Antarctica; Biota; NBP1701; Phytoplankton; R/v Nathaniel B. Palmer; Specific Growth Rate; Thermal Optimum Temperature", "people": "Bishop, Ian", "repository": "USAP-DC", "science_program": null, "title": "Specific growth rate measurements for 43 Southern Ocean diatoms", "url": "https://www.usap-dc.org/view/dataset/601586"}], "date_created": "Fri, 29 Dec 2017 00:00:00 GMT", "description": "The research will examine how diatoms (an important group of plankton in the Southern Ocean) adapt to environmental change. Diatoms will be sampled from different regions of the Southern Ocean, including the Drake Passage, the Pacific Sector of the Southern Ocean and the Ross Sea and examined to determine the range of genetic variation among diatoms in these regions. Experiments on a range of diatoms will be conducted in home laboratories and will be aimed at measuring shifts in physiological capacities over many generations in response to directional changes in the environment (temperature and pH). The information on the genetic diversity of field populations combined with information on potential rates of adaptability and genome changes will provide insight into ways in which polar marine diatoms populations may respond to environmental changes that may occur in surface oceans in the future or may have occurred during past climate conditions. Such information allows better modeling of biogeochemical cycles in the ocean as well as improves our abilities to interpret records of past ocean conditions. The project will support a doctoral student and a postdoctoral researcher as well as several undergraduate students. These scientists will learn the fundamentals of experimental evolution, a skill set that is being sought in the fields of biology and oceanography. The project also includes a collaboration with the Metcalf Institute for Marine and Environmental Reporting that will design and facilitate a session focused on current research related to evolution and climate change to be held at the annual conference of the National Association of Science Writers (NASW). Both physiological and genetic variation are key parameters for understanding evolutionary processes in phytoplankton but they are essentially unknown for Southern Ocean diatoms. The extent to which these two factors determine plasticity and adaptability in field populations and the interaction between them will influence how and whether cold-adapted diatoms can respond to changing environments. This project includes a combination of field work to identify genetic diversity within diatoms using molecular approaches and experiments in the lab to assess the range of physiological variation in contemporary populations of diatoms and evolution experiments in the lab to assess how the combination of genetic diversity and physiological variation influence the evolutionary potential of diatoms under a changing environment. This research will uncover general relationships between physiological variation, genetic diversity, and evolutionary potential that may apply across microbial taxa and geographical regions, substantially improving efforts to predict shifts in marine ecosystems. Results from this study can be integrated into developing models that incorporate evolution to predict ecosystem changes under future climate change scenarios.", "east": null, "geometry": null, "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; IN SITU/LABORATORY INSTRUMENTS \u003e CHEMICAL METERS/ANALYZERS \u003e FLUOROMETERS; IN SITU/LABORATORY INSTRUMENTS \u003e MAGNETIC/MOTION SENSORS \u003e GRAVIMETERS \u003e GRAVIMETERS; IN SITU/LABORATORY INSTRUMENTS \u003e RECORDERS/LOGGERS \u003e AWS; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; EARTH REMOTE SENSING INSTRUMENTS \u003e ACTIVE REMOTE SENSING \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ECHO SOUNDERS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e MBES; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e THERMOSALINOGRAPHS", "is_usap_dc": true, "keywords": "USAP-DC; NBP1701; R/V NBP; AMD; USA/NSF; Amd/Us; DIATOMS", "locations": null, "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Rynearson, Tatiana; Bishop, Ian", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repo": "R2R", "repositories": "NCBI; R2R; USAP-DC", "science_programs": null, "south": null, "title": "NSFGEO-NERC: Evolutionary Response of Southern Ocean Diatoms to Environmental Change", "uid": "p0000850", "west": null}, {"awards": "1344349 Tulaczyk, Slawek; 1344348 Mikucki, Jill", "bounds_geometry": null, "dataset_titles": "2011 Time-domain ElectroMagnetics data for McMurdo Dry Valleys; Marinobacter lipolyticus BF04_CF-4 genomic scaffold, whole genome shotgun sequence; Marinobacter sp. BF14_3D 16S ribosomal RNA gene, partial sequence", "datasets": [{"dataset_uid": "000196", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Marinobacter sp. BF14_3D 16S ribosomal RNA gene, partial sequence", "url": "https://www.ncbi.nlm.nih.gov/nuccore/KX364066"}, {"dataset_uid": "601071", "doi": "10.15784/601071", "keywords": "Antarctica; Dry Valleys; Electromagnetic Data; Geology/Geophysics - Other; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; McMurdo", "people": "Tulaczyk, Slawek", "repository": "USAP-DC", "science_program": null, "title": "2011 Time-domain ElectroMagnetics data for McMurdo Dry Valleys", "url": "https://www.usap-dc.org/view/dataset/601071"}, {"dataset_uid": "000197", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Marinobacter lipolyticus BF04_CF-4 genomic scaffold, whole genome shotgun sequence", "url": "https://www.ncbi.nlm.nih.gov/nuccore?term=PRJNA165567"}], "date_created": "Wed, 08 Nov 2017 00:00:00 GMT", "description": "Intellectual Merit: The MCM-SkyTEM project mapped resistivity in the McMurdo Dry Valleys and at Cape Barne on the Ross Island during the 2011-12 austral season using an airborne transient electromagnetic method. The SkyTEM system is mounted to a helicopter enabling a broad geophysical survey of subsurface resistivity structure over terrain that is inaccessible to traditional ground-based methods. Resistivity measurements obtained distinguish between highly resistive geologic materials such as glacier ice, bedrock and permafrost, and conductive materials such as unfrozen sediments or permafrost with liquid brine to depths of about 300 m. The PIs request funding to derive data products relevant to physical and chemical conditions in potential subsurface microbial habitats of the McMurdo Dry Valleys, similar cold regions on Earth and other planetary bodies. They will use these data products to characterize the hydrologic history of McMurdo Dry Valleys as well as the subsurface hydrologic connectivity in the region to investigate the implications for nutrient and microbial transport. The PIs will make these data products accessible to the research community. Broader impacts: Polar microbial habitats are of high societal and scientific interest because they represent important testing grounds for the limits of life on Earth and other planetary bodies. Project deliverables will include teaching aids for undergraduate and graduate students. Two Ph.D. students will obtain advanced research training as part of this project. The PIs and students on this project will also engage in informal public outreach opportunities by presenting at local K-12 schools and reaching out to local media outlets on stories relating to SkyTEM research.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": null, "nsf_funding_programs": "Antarctic Earth Sciences; Antarctic Integrated System Science; Antarctic Earth Sciences; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Tulaczyk, Slawek; Mikucki, Jill", "platforms": "Not provided", "repo": "NCBI GenBank", "repositories": "NCBI GenBank; USAP-DC", "science_programs": null, "south": null, "title": "Collaborative Research: EAGER: Processing, Interpretation and Dissemination of the Proof-of-Concept Transient Electromagnetic Survey of the McMurdo Dry Valleys Region", "uid": "p0000329", "west": null}, {"awards": "1103428 Thurber, Andrew", "bounds_geometry": "POLYGON((165 -77,165.5 -77,166 -77,166.5 -77,167 -77,167.5 -77,168 -77,168.5 -77,169 -77,169.5 -77,170 -77,170 -77.1,170 -77.2,170 -77.3,170 -77.4,170 -77.5,170 -77.6,170 -77.7,170 -77.8,170 -77.9,170 -78,169.5 -78,169 -78,168.5 -78,168 -78,167.5 -78,167 -78,166.5 -78,166 -78,165.5 -78,165 -78,165 -77.9,165 -77.8,165 -77.7,165 -77.6,165 -77.5,165 -77.4,165 -77.3,165 -77.2,165 -77.1,165 -77))", "dataset_titles": "McMurdo Spiophanes beds 16s V4 region community composition from sediment cores at McMurdo Station, Antarctia on Sept 9th, 2012 (McMurdo Benthos project); Stable isotopic composition of McMurdo Benthos", "datasets": [{"dataset_uid": "000201", "doi": "", "keywords": null, "people": null, "repository": "BCO-DMO", "science_program": null, "title": "Stable isotopic composition of McMurdo Benthos", "url": "https://www.bco-dmo.org/dataset/716462"}, {"dataset_uid": "000202", "doi": "", "keywords": null, "people": null, "repository": "BCO-DMO", "science_program": null, "title": "McMurdo Spiophanes beds 16s V4 region community composition from sediment cores at McMurdo Station, Antarctia on Sept 9th, 2012 (McMurdo Benthos project)", "url": "https://www.bco-dmo.org/dataset/716443"}], "date_created": "Tue, 10 Oct 2017 00:00:00 GMT", "description": "The biota of the world\u0027s seafloor is fueled by bursts of seasonal primary production. For food-limited sediment communities to persist, a balance must exist between metazoan consumption of and competition with bacteria, a balance which likely changes through the seasons. Polar marine ecosystems are ideal places to study such complex interactions due to stark seasonal shifts between heterotrophic and autotrophic communities, and temperatures that may limit microbial processing of organic matter. The research will test the following hypotheses: 1) heterotrophic bacteria compete with macrofauna for food; 2) as phytoplankton populations decline macrofauna increasingly consume microbial biomass to sustain their populations; and 3) in the absence of seasonal photosynthetic inputs, macrofaunal biodiversity will decrease unless supplied with microbially derived nutrition. Observational and empirical studies will test these hypotheses at McMurdo Station, Antarctica, where a high-abundance macro-infaunal community is adapted to this boom-and-bust cycle of productivity. The investigator will mentor undergraduates from a predominantly minority-serving institution, in the fields of invertebrate taxonomy and biogeochemistry. The general public and young scientists will be engaged through lectures at local K-12 venues and launch of an interactive website. The results will better inform scientists and managers about the effects of climate change on polar ecosystems and the mechanisms of changing productivity patterns on global biodiversity.", "east": 170.0, "geometry": "POINT(167.5 -77.5)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -77.0, "nsf_funding_programs": null, "paleo_time": null, "persons": "Thurber, Andrew", "platforms": "Not provided", "repo": "BCO-DMO", "repositories": "BCO-DMO", "science_programs": null, "south": -78.0, "title": "PostDoctoral Research Fellowship", "uid": "p0000416", "west": 165.0}, {"awards": "1141993 Rich, Jeremy", "bounds_geometry": "POLYGON((-60 -70,-59.3 -70,-58.6 -70,-57.9 -70,-57.2 -70,-56.5 -70,-55.8 -70,-55.1 -70,-54.4 -70,-53.7 -70,-53 -70,-53 -70.9,-53 -71.8,-53 -72.7,-53 -73.6,-53 -74.5,-53 -75.4,-53 -76.3,-53 -77.2,-53 -78.1,-53 -79,-53.7 -79,-54.4 -79,-55.1 -79,-55.8 -79,-56.5 -79,-57.2 -79,-57.9 -79,-58.6 -79,-59.3 -79,-60 -79,-60 -78.1,-60 -77.2,-60 -76.3,-60 -75.4,-60 -74.5,-60 -73.6,-60 -72.7,-60 -71.8,-60 -70.9,-60 -70))", "dataset_titles": "Seasonal Succession of Bacterial Communities in Coastal Waters of the Western Antarctic Peninsula", "datasets": [{"dataset_uid": "601032", "doi": "10.15784/601032", "keywords": "Antarctica; Antarctic Peninsula; Bacteria; Biota; Genetic; Geochemistry; Palmer Station; Sample/collection Description; Sample/Collection Description; Sea Water; Southern Ocean", "people": "Rich, Jeremy", "repository": "USAP-DC", "science_program": null, "title": "Seasonal Succession of Bacterial Communities in Coastal Waters of the Western Antarctic Peninsula", "url": "https://www.usap-dc.org/view/dataset/601032"}], "date_created": "Thu, 15 Jun 2017 00:00:00 GMT", "description": "The Western Antarctic Peninsula (WAP) has experienced unprecedented warming and shifts in sea ice cover over the past fifty years. How these changes impact marine microbial communities, and subsequently how these shifts in the biota may affect the carbon cycle in surface waters is unknown. This work will examine how these ecosystem-level changes affect microbial community structure and function. This research will use modern metagenomic and transcriptomic approaches to test the hypothesis that the introduction of organic matter from spring phytoplankton blooms drives turnover in microbial communities. This research will characterize patterns in bacterial and archaeal succession during the transition from the austral winter at two long-term monitoring sites: Palmer Station in the north and Rothera Station in the south. This project will also include microcosm incubations to directly assess the effects of additions of organic carbon and melted sea ice on microbial community structure and function. The results of this work will provide a broader understanding of the roles of both rare and abundant microorganisms in carbon cycling within the WAP region, and how these communities may shift in structure and function in response to climate change. Results will be widely disseminated through publications as well as through presentations at national and international meetings. The research will provide training opportunities for both graduate and undergraduate students and will enhance international collaborations with the British Antarctic Survey.", "east": -53.0, "geometry": "POINT(-56.5 -74.5)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -70.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Rich, Jeremy", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -79.0, "title": "Collaborative Research: Microbial Community Assembly in Coastal Waters of the Western Antarctic Peninsula", "uid": "p0000409", "west": -60.0}, {"awards": "1246223 Hastings, Meredith", "bounds_geometry": "POINT(-112.1115 -79.481)", "dataset_titles": "WAIS Divide WDC06A Nitrate Isotope Record", "datasets": [{"dataset_uid": "601022", "doi": "10.15784/601022", "keywords": "Antarctica; Chemistry:ice; Chemistry:Ice; Geochemistry; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Ice Core Records; Nitrate; WAIS Divide; WAIS Divide Ice Core", "people": "Hastings, Meredith; Buffen, Aron", "repository": "USAP-DC", "science_program": "WAIS Divide Ice Core", "title": "WAIS Divide WDC06A Nitrate Isotope Record", "url": "https://www.usap-dc.org/view/dataset/601022"}], "date_created": "Tue, 02 May 2017 00:00:00 GMT", "description": "Hastings/1246223 This award supports a project with the aim of distinguishing the sources of nitrate deposition to the West Antarctic Ice Sheet (WAIS) using isotopic ratios snow in archive snow and ice samples. The isotopic composition of nitrate has been shown to contain information about the source of the nitrate (i.e. nitrogen oxides = NOx = NO+NO2) and the oxidation processes that convert NOx to nitrate in the atmosphere prior to deposition. A difficulty in interpreting records in the context of NOx sources is that nitrate can be post-depositionally processed in surface snow, such that the archived record does not reflect the composition of the atmosphere. This intellectual merit of this work specifically aims to investigate variability in the isotopic composition of nitrate in snow and ice from the WAIS in the context of accumulation rate, NOx source emissions, and atmospheric chemistry. These records will be interpreted in the context of our understanding of biospheric (biomass burning, microbial processes in soils), atmospheric (lightning, transport, chemistry), and climate (temperature, accumulation rate) changes over time. A graduate student will be supported as part of this project, and both graduate student and PI will be involved in communicating the utility and results of polar research to elementary school students in the Providence, RI area. The broader impacts of the project also include making efforts to attract more young, female scientists to polar research by establishing a connection between the Earth Science Women\u0027s Network (ESWN), an organization PI Hastings helped to establish, and the Association of Polar Early Career Scientists (APECS). Finally, results of all measurements will be presented at relevant conferences, made available publicly and published in peer-reviewed journals.", "east": -112.1115, "geometry": "POINT(-112.1115 -79.481)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -79.481, "nsf_funding_programs": "Antarctic Glaciology", "paleo_time": null, "persons": "Hastings, Meredith", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": "WAIS Divide Ice Core", "south": -79.481, "title": "Investigating Source, Chemistry and Climate changes using the Isotopic Composition of Nitrate in Antarctic Snow and Ice", "uid": "p0000399", "west": -112.1115}, {"awards": "1141978 Foreman, Christine", "bounds_geometry": "POLYGON((160 -76,160.1 -76,160.2 -76,160.3 -76,160.4 -76,160.5 -76,160.6 -76,160.7 -76,160.8 -76,160.9 -76,161 -76,161 -76.1,161 -76.2,161 -76.3,161 -76.4,161 -76.5,161 -76.6,161 -76.7,161 -76.8,161 -76.9,161 -77,160.9 -77,160.8 -77,160.7 -77,160.6 -77,160.5 -77,160.4 -77,160.3 -77,160.2 -77,160.1 -77,160 -77,160 -76.9,160 -76.8,160 -76.7,160 -76.6,160 -76.5,160 -76.4,160 -76.3,160 -76.2,160 -76.1,160 -76))", "dataset_titles": "FT-ICR MS Metadata; Respiration Metadata; UPLC-Q-TOF data of Cotton Glacier exometabolites", "datasets": [{"dataset_uid": "601089", "doi": "10.15784/601089", "keywords": "Antarctica; Biota; Exometabolites; Mass Spectrometry; Microbes; Microbiology", "people": "Foreman, Christine; Tigges, Michelle; Bothner, Brian", "repository": "USAP-DC", "science_program": null, "title": "UPLC-Q-TOF data of Cotton Glacier exometabolites", "url": "https://www.usap-dc.org/view/dataset/601089"}, {"dataset_uid": "601077", "doi": "10.15784/601077", "keywords": "Antarctica; Biota; Chemistry:fluid; Chemistry:Fluid; Fluorescence Spectroscopy; Mass Spectrometry", "people": "Foreman, Christine; D\u0027Andrilli, Juliana", "repository": "USAP-DC", "science_program": null, "title": "FT-ICR MS Metadata", "url": "https://www.usap-dc.org/view/dataset/601077"}, {"dataset_uid": "601076", "doi": "10.15784/601076", "keywords": "Antarctica; Biota; Chemistry:fluid; Chemistry:Fluid; Fluorescence Spectroscopy; Mass Spectrometry", "people": "Foreman, Christine; Smith, Heidi", "repository": "USAP-DC", "science_program": null, "title": "Respiration Metadata", "url": "https://www.usap-dc.org/view/dataset/601076"}], "date_created": "Tue, 25 Apr 2017 00:00:00 GMT", "description": "Uncovering the dynamics of dissolved organic matter (DOM) is central to an understanding of the global carbon cycle, as organic material from lakes, streams, oceans and soils passes through this pool. DOM acts as a key energy source for microbes in many ecosystems and therefore can affect regional nutrient cycling patterns. For example, preliminary results suggest that organisms isolated from a supraglacial stream on Cotton Glacier, Antarctica, may be important in DOM cycling in this relatively simple, low temperature system. However, little is known about the functional attributes of the microbes that interact with DOM in the environment. This project will use state-of-the-art genomics, proteomics and metabolomics approaches to understand the mechanisms by which two microbial isolates, CG3 and CG9_1, affect DOM cycling. Liquid chromatography-mass spectrometry will also be used to better characterize the microbially-derived DOM from this ecosystem. This project will support the research and training of one undergraduate and two graduate students. Results will be widely disseminated through publications as well as through presentations at national and international meetings. In addition, raw data will be made available through open-access databases. Understanding the relationship between cold-adapted microbial metabolisms and DOM pools is important as more than 90% of the Earth?s oceans are below 5 degrees Celsius.", "east": 161.0, "geometry": "POINT(160.5 -76.5)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -76.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Foreman, Christine; Bothner, Brian", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -77.0, "title": "Multidimensional \"omics\" characterization of microbial metabolism and dissolved organic matter in Antarctica", "uid": "p0000408", "west": 160.0}, {"awards": "1340905 Doran, Peter", "bounds_geometry": "POLYGON((161 -77,161.3 -77,161.6 -77,161.9 -77,162.2 -77,162.5 -77,162.8 -77,163.1 -77,163.4 -77,163.7 -77,164 -77,164 -77.05,164 -77.1,164 -77.15,164 -77.2,164 -77.25,164 -77.3,164 -77.35,164 -77.4,164 -77.45,164 -77.5,163.7 -77.5,163.4 -77.5,163.1 -77.5,162.8 -77.5,162.5 -77.5,162.2 -77.5,161.9 -77.5,161.6 -77.5,161.3 -77.5,161 -77.5,161 -77.45,161 -77.4,161 -77.35,161 -77.3,161 -77.25,161 -77.2,161 -77.15,161 -77.1,161 -77.05,161 -77))", "dataset_titles": "Lake Bonney Autonomous Lake Profiler and Samplers (ALPS): Particulate Organic Carbon and Nitrogen Concentrations. doi:10.6073/pasta/0043c1728b4e51879970d59f2d0ce575", "datasets": [{"dataset_uid": "002521", "doi": "", "keywords": null, "people": null, "repository": "LTER", "science_program": null, "title": "Lake Bonney Autonomous Lake Profiler and Samplers (ALPS): Particulate Organic Carbon and Nitrogen Concentrations. doi:10.6073/pasta/0043c1728b4e51879970d59f2d0ce575", "url": "http://www.mcmlter.org/node/3957"}], "date_created": "Fri, 13 Jan 2017 00:00:00 GMT", "description": "EAGER: Collaborative Research: Habitability of Antarctic lakes and detectability of microbial life in icy environments by autonomous year-round instrumentation, is supported by the Antarctic Integrated System Science (AISS) and the Antarctic Organisms and Ecosystems (AOE) programs within the Antarctic Sciences section in the Division of Polar Programs within the Geosciences Directorate of the National Sciences Foundation (NSF). The funds will allow the measurement of year-round properties of the microbes and the surrounding water in Lake Bonney, a lake with four meters of permanent ice cover over forty meters of liquid water in the Dry Valleys of Antarctica. NSF funds will be used to support the deployment, and the science enabled by the deployment, and NASA (the National Aeronautics and Space Administration) funds will be used to purchase the equipment. Intellectual Merit: This research will be the first to make year-round measurements of the microbial community, and several associated environmental variables, in the continuously liquid portions of Lake Bonney, Antarctica. Three different types of equipment will be deployed in each of the lobes of Lake Bonney. The first instrument is an ITP (an ice-tethered profiler) that will measure physical parameters such as temperature, dissolved oxygen, and chlorophyll throughout the full depth of the liquid water portion of the lake, making measurements at least once each week. The second and third instruments will be used to collect discrete water samples at least every two weeks to determine A) the biological community (assessing metabolic and phylogenetic diversity) and B) the geochemistry (e.g., dissolved organic carbon, and dissolved inorganic nitrogen species). Such samplers have never been used to measure these properties year-round in the Antarctic. Cold temperatures, bottom lake water salinities that are four times greater than the ocean, the thick permanent ice cover, and the lack of sunlight to recharge batteries all present significant challenges for the project, thus classifying the work as an early, high-risk, high-reward activity (the acronym EAGER stands for Early-concept Grants for Exploratory Research). Broader Impacts: There is much interest in understanding the ecosystems of the Polar regions in an era of climate change. Logistical limitations dictate much of this work only take place in the summer, until new autonomous technologies can open the door for year-round measurements. This award will be the first to attempt year-round microbial sampling in Antarctica. The McMurdo Dry Valleys region is also the site of a Long-Term Ecological Research (LTER) Program, and the research conducted on this project with benefit from, and contribute to, the larger LTER project. The instruments used in the project will be purchased by NASA, so two separate agencies have agreed to explore the feasibility of an early stage project. There will be at least three graduate student trained during the project, and the team will also participate in outreach activities at several venues including the Crow Reservation in Montana.", "east": 164.0, "geometry": "POINT(162.5 -77.25)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -77.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Integrated System Science", "paleo_time": null, "persons": "Doran, Peter; Takacs-Vesbach, Cristina; Priscu, John", "platforms": "Not provided", "repo": "LTER", "repositories": "LTER", "science_programs": "LTER", "south": -77.5, "title": "EAGER: Collaborative Research: Habitability of Antarctic Lakes and Detectability of Microbial Life in Icy Environments by Aautonomous Year-round Instrumentation", "uid": "p0000326", "west": 161.0}, {"awards": "1142102 Takacs-Vesbach, Cristina; 1142096 Schwartz, Egbert", "bounds_geometry": "POLYGON((161 -77,161.3 -77,161.6 -77,161.9 -77,162.2 -77,162.5 -77,162.8 -77,163.1 -77,163.4 -77,163.7 -77,164 -77,164 -77.05,164 -77.1,164 -77.15,164 -77.2,164 -77.25,164 -77.3,164 -77.35,164 -77.4,164 -77.45,164 -77.5,163.7 -77.5,163.4 -77.5,163.1 -77.5,162.8 -77.5,162.5 -77.5,162.2 -77.5,161.9 -77.5,161.6 -77.5,161.3 -77.5,161 -77.5,161 -77.45,161 -77.4,161 -77.35,161 -77.3,161 -77.25,161 -77.2,161 -77.15,161 -77.1,161 -77.05,161 -77))", "dataset_titles": "GenBank. Accession # PRJNA232062,PRJNA228947,PRJNA228945; McMurdo Dry Valleys LTER Genetic/Genomic Data Resource; NCBI GenBank RNA sequences", "datasets": [{"dataset_uid": "000177", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "NCBI GenBank RNA sequences", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA356879"}, {"dataset_uid": "000178", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "GenBank. Accession # PRJNA232062,PRJNA228947,PRJNA228945", "url": "http://www.ncbi.nlm.nih.gov/genbank/"}, {"dataset_uid": "000180", "doi": "", "keywords": null, "people": null, "repository": "LTER", "science_program": null, "title": "McMurdo Dry Valleys LTER Genetic/Genomic Data Resource", "url": "http://www.mcmlter.org/genetic"}], "date_created": "Wed, 04 Jan 2017 00:00:00 GMT", "description": "The McMurdo Dry Valleys in Antarctica are among the coldest, driest habitats on the planet. Previous research has documented the presence of surprisingly diverse microbial communities in the soils of the Dry Valleys despite these extreme conditions. However, the degree to which these organisms are active is unknown; it is possible that much of this diversity reflects microbes that have blown into this environment that are subsequently preserved in these cold, dry conditions. This research will use modern molecular techniques to answer a fundamental question regarding these communities: which organisms are active and how do they live in such extreme conditions? The research will include manipulations to explore how changes in water, salt and carbon affect the microbial community, to address the role that these organisms play in nutrient cycling in this environment. The results of this work will provide a broader understanding of how life adapts to such extreme conditions as well as the role of dormancy in the life history of microorganisms. Results will be widely disseminated through publications as well as through presentations at national and international meetings; raw data will be made available through a high-profile web-based portal. The research will support two graduate students, two undergraduate research assistants and a postdoctoral fellow. The results will be incorporated into a webinar targeted to secondary and post-secondary educators and a complimentary hands-on class activity kit will be developed and made available to various teacher and outreach organizations.", "east": 164.0, "geometry": "POINT(162.5 -77.25)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -77.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Takacs-Vesbach, Cristina; Schwartz, Egbert; Van Horn, David", "platforms": "Not provided", "repo": "NCBI GenBank", "repositories": "LTER; NCBI GenBank", "science_programs": "LTER", "south": -77.5, "title": "Collaborative Research: An Integrated Ecological Investigation of McMurdo Dry Valley\u0027s Active Soil Microbial Communities", "uid": "p0000334", "west": 161.0}, {"awards": "1141936 Foreman, Christine", "bounds_geometry": "POINT(112.085 -79.467)", "dataset_titles": "Molecular Level Characterization of Dissolved Organic Carbon and Microbial Diversity in the WAIS Divide Replicate Core", "datasets": [{"dataset_uid": "600133", "doi": "10.15784/600133", "keywords": "Antarctica; Biota; Genetic Sequences; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Ice Core Records; Paleoclimate; WAIS Divide; WAIS Divide Ice Core", "people": "Foreman, Christine", "repository": "USAP-DC", "science_program": "WAIS Divide Ice Core", "title": "Molecular Level Characterization of Dissolved Organic Carbon and Microbial Diversity in the WAIS Divide Replicate Core", "url": "https://www.usap-dc.org/view/dataset/600133"}], "date_created": "Thu, 05 Nov 2015 00:00:00 GMT", "description": "This award supports a detailed, molecular level characterization of dissolved organic carbon and microbes in Antarctic ice cores. Using the most modern biological (genomic), geochemical techniques, and advanced chemical instrumentation researchers will 1) optimize protocols for collecting, extracting and amplifying DNA from deep ice cores suitable for use in next generation pyrosequencing; 2) determine the microbial diversity within the ice core; and 3) obtain and analyze detailed molecular characterizations of the carbon in the ice by ultrahigh resolution Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS). With this pilot study investigators will be able to quantify the amount of material (microbial biomass and carbon) required to perform these characterizations, which is needed to inform future ice coring projects. The ultimate goal will be to develop protocols that maximize the yield, while minimizing the amount of ice required. The broader impacts include education and outreach at both the local and national levels. As a faculty mentor with the American Indian Research Opportunities and BRIDGES programs at Montana State University, Foreman will serve as a mentor to a Native American student in the lab during the summer months. Susan Kelly is an Education and Outreach Coordinator with a MS degree in Geology and over 10 years of experience in science outreach. She will coordinate efforts for comprehensive educational collaboration with the Hardin School District on the Crow Indian Reservation in South-central Montana.", "east": 112.085, "geometry": "POINT(112.085 -79.467)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e CHEMICAL METERS/ANALYZERS \u003e ADS; IN SITU/LABORATORY INSTRUMENTS \u003e CORERS \u003e CORING DEVICES; IN SITU/LABORATORY INSTRUMENTS \u003e SPECTROMETERS/RADIOMETERS \u003e MASS SPECTROMETERS", "is_usap_dc": true, "keywords": "Dissolved Organic Carbon; Microbes; Ice Core; Not provided; Pyrosequencing; Microbial Diversity; Molecular; WAIS Divide; LABORATORY; FIELD SURVEYS; Antarctic; FIELD INVESTIGATION; DNA", "locations": "Antarctic; WAIS Divide", "north": -79.467, "nsf_funding_programs": "Antarctic Glaciology", "paleo_time": null, "persons": "Foreman, Christine", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION; LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS; Not provided; OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": "WAIS Divide Ice Core", "south": -79.467, "title": "Molecular Level Characterization of Dissolved Organic Carbon and Microbial Diversity in the WAIS Divide Replicate Core", "uid": "p0000342", "west": 112.085}, {"awards": "0944556 Barrett, John", "bounds_geometry": "POLYGON((160.6015 -76.9089,161.7382 -76.9089,162.8749 -76.9089,164.0116 -76.9089,165.1483 -76.9089,166.285 -76.9089,167.4217 -76.9089,168.5584 -76.9089,169.6951 -76.9089,170.8318 -76.9089,171.9685 -76.9089,171.9685 -77.73527,171.9685 -78.56164,171.9685 -79.38801,171.9685 -80.21438,171.9685 -81.04075,171.9685 -81.86712,171.9685 -82.69349,171.9685 -83.51986,171.9685 -84.34623,171.9685 -85.1726,170.8318 -85.1726,169.6951 -85.1726,168.5584 -85.1726,167.4217 -85.1726,166.285 -85.1726,165.1483 -85.1726,164.0116 -85.1726,162.8749 -85.1726,161.7382 -85.1726,160.6015 -85.1726,160.6015 -84.34623,160.6015 -83.51986,160.6015 -82.69349,160.6015 -81.86712,160.6015 -81.04075,160.6015 -80.21438,160.6015 -79.38801,160.6015 -78.56164,160.6015 -77.73527,160.6015 -76.9089))", "dataset_titles": "Ecosphere (Supplement), Ecological Society of America.", "datasets": [{"dataset_uid": "002538", "doi": "", "keywords": null, "people": null, "repository": "Publication", "science_program": null, "title": "Ecosphere (Supplement), Ecological Society of America.", "url": "http://www.esapubs.org/archive/ecos/C004/014/suppl-1.php"}], "date_created": "Fri, 13 Feb 2015 00:00:00 GMT", "description": "Advances in molecular techniques have expanded our understanding of soil microbial communities, and raised important questions about regional and global patterns in microbial diversity. The proposed research will investigate the composition and activity of microbial communities across a range of geochemical and hydrologic soil conditions, and over local to regional scales in the Transantarctic Mountains, in order to assess controls over microbial biogeography. The research targets two areas in the Transantarctic mountains, the McMurdo Dry Valleys, and the Beardmore Glacier region further south, the latter representing an underexplored and inarguably more extreme soil environment. The research project will adopt an integrated approach, using molecular techniques and in situ assessment of biological activity in a quantitative biogeographical framework, with the goal of distinguishing fine versus broad scale controls over microbial community structure. The research is essential to determining the basic trophic status of extreme microbial food webs, and their sensitivity to climate change. The investigators will engage secondary and post-secondary educators through first person outreach as well as web-based communications and exercises. Two postdoctoral scientists will be trained in an interdisciplinary and international setting.", "east": 171.9685, "geometry": "POINT(166.285 -81.04075)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -76.9089, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Barrett, John", "platforms": "Not provided", "repo": "Publication", "repositories": "Publication", "science_programs": null, "south": -85.1726, "title": "Collaborative Research: Controls over the Spatial Distribution and Activity of Microbial Communities in Antarctic Soils", "uid": "p0000350", "west": 160.6015}, {"awards": "0944727 Arrigo, Kevin", "bounds_geometry": "POLYGON((-118.3 -71.6,-117.57 -71.6,-116.84 -71.6,-116.11 -71.6,-115.38 -71.6,-114.65 -71.6,-113.92 -71.6,-113.19 -71.6,-112.46 -71.6,-111.73 -71.6,-111 -71.6,-111 -71.86,-111 -72.12,-111 -72.38,-111 -72.64,-111 -72.9,-111 -73.16,-111 -73.42,-111 -73.68,-111 -73.94,-111 -74.2,-111.73 -74.2,-112.46 -74.2,-113.19 -74.2,-113.92 -74.2,-114.65 -74.2,-115.38 -74.2,-116.11 -74.2,-116.84 -74.2,-117.57 -74.2,-118.3 -74.2,-118.3 -73.94,-118.3 -73.68,-118.3 -73.42,-118.3 -73.16,-118.3 -72.9,-118.3 -72.64,-118.3 -72.38,-118.3 -72.12,-118.3 -71.86,-118.3 -71.6))", "dataset_titles": "Dataset: Chlorophyll a", "datasets": [{"dataset_uid": "000172", "doi": "", "keywords": null, "people": null, "repository": "BCO-DMO", "science_program": null, "title": "Dataset: Chlorophyll a", "url": "http://www.bco-dmo.org/dataset/546372"}], "date_created": "Fri, 30 Jan 2015 00:00:00 GMT", "description": "ASPIRE is an NSF-funded project that will examine the ecology of the Amundsen Sea during the Austral summer of 2010. ASPIRE includes an international team of trace metal and carbon chemists, phytoplankton physiologists, microbial and zooplankton ecologists, and physical oceanographers, that will investigate why and how the Amundsen Sea Polynya is so much more productive than other polynyas and whether interannual variability can provide insight to climate-sensitive mechanisms driving carbon fluxes. This project will compliment the existing ASPIRE effort by using 1) experimental manipulations to understand photoacclimation of the dominant phytoplankton taxa under conditions of varying light and trace metal abundance, 2) nutrient addition bioassays to determine the importance of trace metal versus nitrogen limitation of phytoplankton growth, and 3) a numerical ecosystem model to understand the importance of differences in mixing regime, flow field, and Fe sources in controlling phytoplankton bloom dynamics and community composition in this unusually productive polynya system. The research strategy will integrate satellite remote sensing, field-based experimental manipulations, and numerical modeling. Outreach and education include participation in Stanford\u0027s Summer Program for Professional Development for Science Teachers, Stanford\u0027s School of Earth Sciences high school internship program, and development of curriculum for local science training centers, including the Chabot Space and Science Center. Undergraduate participation and training will include support for both graduate students and undergraduate assistants.", "east": -111.0, "geometry": "POINT(-114.65 -72.9)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -71.6, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Arrigo, Kevin", "platforms": "Not provided", "repo": "BCO-DMO", "repositories": "BCO-DMO", "science_programs": null, "south": -74.2, "title": "ASPIRE: Amundsen Sea Polynya International Research Expedition", "uid": "p0000348", "west": -118.3}, {"awards": "0838970 Foreman, Christine", "bounds_geometry": "POINT(161.667 -77.117)", "dataset_titles": "The Biogeochemical Evolution of Dissolved Organic Matter in a Fluvial System on the Cotton Glacier, Antarctica", "datasets": [{"dataset_uid": "600104", "doi": "10.15784/600104", "keywords": "Antarctica; Biota; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Microbiology", "people": "Foreman, Christine", "repository": "USAP-DC", "science_program": null, "title": "The Biogeochemical Evolution of Dissolved Organic Matter in a Fluvial System on the Cotton Glacier, Antarctica", "url": "https://www.usap-dc.org/view/dataset/600104"}], "date_created": "Fri, 10 Oct 2014 00:00:00 GMT", "description": "Dissolved organic matter (DOM) comprises a significant pool of Earth\u0027s organic carbon that dwarfs the amount present in living aquatic organisms. The properties and reactivity of DOM are not well defined, and the evolution of autochthonous DOM from its precursor materials in freshwater has not been observed. Recent sampling of a supraglacial stream formed on the Cotton Glacier in the Transantarctic Mountains revealed DOM that more closely resembles an assemblage of recognizable precursor organic compounds, based upon its UV-VIS and fluorescence spectra. It is suggested that the DOM from this water evolved over time to resemble materials present in marine and many inland surface waters. The transient nature of the system i.e., it reforms seasonally, also prevents any accumulation of the refractory DOM present in most surface waters. Thus, the Cotton Glacier provides us with a unique environment to study the formation of DOM from precursor materials. An interdisciplinary team will study the biogeochemistry of this progenitor DOM and how microbes modify it. By focusing on the chemical composition of the DOM as it shifts from precursor material to the more humified fractions, the investigators will relate this transition to bioavailability, enzymatic activity, community composition and microbial growth efficiency. This project will support education at all levels, K-12, high school, undergraduate, graduate and post-doc and will increase participation by under-represented groups in science. Towards these goals, the investigators have established relationships with girls\u0027 schools and Native American programs. Additional outreach will be carried out in coordination with PolarTREC, PolarPalooza, and if possible, an Antarctic Artist and Writer.", "east": 161.667, "geometry": "POINT(161.667 -77.117)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -77.117, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Foreman, Christine", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -77.117, "title": "Collaborative Research: The Biogeochemical Evolution of Dissolved Organic Matter in a Fluvial System on the Cotton Glacier, Antarctica", "uid": "p0000458", "west": 161.667}, {"awards": "1043265 Deming, Jody", "bounds_geometry": "POLYGON((162.1397 -77.14085,162.828507 -77.14085,163.517314 -77.14085,164.206121 -77.14085,164.894928 -77.14085,165.583735 -77.14085,166.272542 -77.14085,166.961349 -77.14085,167.650156 -77.14085,168.338963 -77.14085,169.02777 -77.14085,169.02777 -77.200745,169.02777 -77.26064,169.02777 -77.320535,169.02777 -77.38043,169.02777 -77.440325,169.02777 -77.50022,169.02777 -77.560115,169.02777 -77.62001,169.02777 -77.679905,169.02777 -77.7398,168.338963 -77.7398,167.650156 -77.7398,166.961349 -77.7398,166.272542 -77.7398,165.583735 -77.7398,164.894928 -77.7398,164.206121 -77.7398,163.517314 -77.7398,162.828507 -77.7398,162.1397 -77.7398,162.1397 -77.679905,162.1397 -77.62001,162.1397 -77.560115,162.1397 -77.50022,162.1397 -77.440325,162.1397 -77.38043,162.1397 -77.320535,162.1397 -77.26064,162.1397 -77.200745,162.1397 -77.14085))", "dataset_titles": null, "datasets": null, "date_created": "Thu, 31 Jul 2014 00:00:00 GMT", "description": "The relatively pristine Antarctic continent with its extensive maritime zone represents a unique location on the planet to investigate the long distance aerial transport and deposition of marine microorganisms. The vast extent of new sea ice that forms each winter around the continent results in large numbers of frost flowers, delicate ice-crystal structures of high salt content that form on the surface of the ice and are readily dispersed by wind. The proposed research builds on earlier work in the Arctic and tests the new hypothesis that wind-borne frost flowers provide an effective mechanism for the transport of marine bacteria over long distances, one that can be uniquely sourced and tracked by the frost flower salt signature in the Antarctic realm. A highly resolved genomic snapshot of the microbial community will be acquired at each stage in the transport path, which will track decreasing fractions of the marine microbial community as it freezes into sea ice, incorporates into frost flowers, converts to aerosols, and ultimately deposits within continental snowpack. En route from sea ice to snowpack, marine bacteria will be exposed to an array of environmental stresses, including high salinity, low temperatures, UV light and potential desiccation. A parallel proteomic analysis will enable an evaluation of the microbial response to these extreme conditions and potential survival mechanisms that allow persistence or eventual colonization of deposition sites across Antarctica. Current understanding of microbes in the Antarctic atmosphere is based on a limited number of microscopic and culture-based assays and a single report of low-resolution 16S RNA gene sequence analysis. The research will broadly impact understanding of atmospheric microbiology, from source to deposition, and various issues of microbial survival, colonization, endemism, and diversity under extreme conditions. In addition to venues that reach the scientific community, the research team will develop a permanent multi-media and artifact-based exhibit on Antarctic Microbial Transport that will be showcased at Seattle\u0027s Pacific Science Center (PSC), which educates nearly a million visitors annually.", "east": 169.02777, "geometry": "POINT(165.583735 -77.440325)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -77.14085, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Deming, Jody", "platforms": "Not provided", "repositories": null, "science_programs": null, "south": -77.7398, "title": "High Resolution Genomic and Proteomic Analyses of a Microbial Transport Mechanism from Antarctic Marine Waters to Permanent Snowpack", "uid": "p0000356", "west": 162.1397}, {"awards": "1043740 Lenczewski, Melissa", "bounds_geometry": "POLYGON((165 -77.5,165.3 -77.5,165.6 -77.5,165.9 -77.5,166.2 -77.5,166.5 -77.5,166.8 -77.5,167.1 -77.5,167.4 -77.5,167.7 -77.5,168 -77.5,168 -77.6,168 -77.7,168 -77.8,168 -77.9,168 -78,168 -78.1,168 -78.2,168 -78.3,168 -78.4,168 -78.5,167.7 -78.5,167.4 -78.5,167.1 -78.5,166.8 -78.5,166.5 -78.5,166.2 -78.5,165.9 -78.5,165.6 -78.5,165.3 -78.5,165 -78.5,165 -78.4,165 -78.3,165 -78.2,165 -78.1,165 -78,165 -77.9,165 -77.8,165 -77.7,165 -77.6,165 -77.5))", "dataset_titles": "Fate of Drilling Fluids during the South McMurdo Sound Project (SMS) of the Antarctic Geological Drilling Program (ANDRILL)", "datasets": [{"dataset_uid": "600129", "doi": "10.15784/600129", "keywords": "Andrill; Antarctica; Chemistry:fluid; Chemistry:Fluid; Chemistry:rock; Chemistry:Rock; Drilling Fluid; Geochemistry; McMurdo; Ross Sea; Sediment Core", "people": "Lenczewski, Melissa", "repository": "USAP-DC", "science_program": "ANDRILL", "title": "Fate of Drilling Fluids during the South McMurdo Sound Project (SMS) of the Antarctic Geological Drilling Program (ANDRILL)", "url": "https://www.usap-dc.org/view/dataset/600129"}], "date_created": "Mon, 27 Jan 2014 00:00:00 GMT", "description": "Intellectual Merit: The PI proposes to utilize computer models used by hydrogeologists to establish the fate and transport of contamination and determine the extent of drilling fluid contamination in the ANDRILL SMS core. For these models, previously collected logs of lithology, porosity, fracture density, fracture type, fracture orientation, drilling fluid loss, drilling fluid characteristics and temperature will be used as input parameters. In addition, biodegradation and sorption constants for the drilling fluid will be determined and incorporated into the models. Samples of drilling fluids used during coring as well as the return fluids were collected at the drill site using standard microbiological sampling techniques. Fluids will be tested at in situ temperatures under aerobic and anaerobic conditions to determine biodegradation constants. Sorption will be determined between the drilling fluids and core samples using standard isotherm methods. Geochemical and microbial fingerprints of the fluids and the changes during biodegradation will determine the potential impact of the drilling fluids on the isolated microbial communities and the geochemistry within various subsurface lithologic units beneath the southern McMurdo Sound in Antarctica. The results of this study could potentially provide guidelines on developing less detrimental methods for future exploration, if deemed necessary through this research. Broader impacts: This proposed project will train a graduate student. The methods developed for analyses of samples in this project will serve as a guide for future studies of similar interest and will improve the understanding of ecological impacts of geologic drilling in Antarctica. The results of this study will be used as a reference for comparison with future studies examining newly developed, and improved, sample collection methods in future exploratory drilling projects in pristine environments. The PI is new to Antarctic research.", "east": 168.0, "geometry": "POINT(166.5 -78)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -77.5, "nsf_funding_programs": "Antarctic Earth Sciences", "paleo_time": null, "persons": "Lenczewski, Melissa", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": "ANDRILL", "south": -78.5, "title": "Fate of Drilling Fluids during the South McMurdo Sound Project (SMS) of the Antarctic Geological Drilling Program (ANDRILL)", "uid": "p0000468", "west": 165.0}, {"awards": "0838830 Cottrell, Matthew", "bounds_geometry": "POLYGON((-64.079666 -64.77966,-64.07576590000001 -64.77966,-64.0718658 -64.77966,-64.0679657 -64.77966,-64.0640656 -64.77966,-64.06016550000001 -64.77966,-64.0562654 -64.77966,-64.0523653 -64.77966,-64.04846520000001 -64.77966,-64.0445651 -64.77966,-64.040665 -64.77966,-64.040665 -64.78326100000001,-64.040665 -64.786862,-64.040665 -64.790463,-64.040665 -64.794064,-64.040665 -64.797665,-64.040665 -64.801266,-64.040665 -64.804867,-64.040665 -64.808468,-64.040665 -64.812069,-64.040665 -64.81567,-64.0445651 -64.81567,-64.04846520000001 -64.81567,-64.0523653 -64.81567,-64.0562654 -64.81567,-64.06016550000001 -64.81567,-64.0640656 -64.81567,-64.0679657 -64.81567,-64.0718658 -64.81567,-64.07576590000001 -64.81567,-64.079666 -64.81567,-64.079666 -64.812069,-64.079666 -64.808468,-64.079666 -64.804867,-64.079666 -64.801266,-64.079666 -64.797665,-64.079666 -64.794064,-64.079666 -64.790463,-64.079666 -64.786862,-64.079666 -64.78326100000001,-64.079666 -64.77966))", "dataset_titles": "Photoheterotrophic Microbes in the West Antarctic Peninsula Marine Ecosystem", "datasets": [{"dataset_uid": "600097", "doi": "10.15784/600097", "keywords": "Antarctic Peninsula; Biota; LTER Palmer Station; Microbiology; Oceans; Southern Ocean", "people": "Kirchman, David; Cottrell, Matthew", "repository": "USAP-DC", "science_program": null, "title": "Photoheterotrophic Microbes in the West Antarctic Peninsula Marine Ecosystem", "url": "https://www.usap-dc.org/view/dataset/600097"}], "date_created": "Mon, 16 Dec 2013 00:00:00 GMT", "description": "This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).\u003cbr/\u003e\u003cbr/\u003eLight quality and availability are likely to change in polar ecosystems as ice coverage and thickness decrease. How microbes adjust to these and other changes will have huge impacts on the polar marine ecosystems. Little is known about photoheterotrophic prokaryotes, which are hypothesized to gain a metabolic advantage by harvesting light energy in addition to utilizing dissolved organic matter (DOM). Photoheterotrophy is not included in current models of carbon cycling and energy flow. This research will examine three questions: 1. Are photoheterotrophic microbes present and active in Antarctic waters in winter and summer? 2. Does community structure of photoheterotrophs shift between summer and winter? 3. Which microbial groups assimilate more DOM in light than in the dark? The research will test hypotheses about activity of photoheterotrophs in winter and in summer, shifts in community structure between light and dark seasons and the potentially unique impacts of photoheterotrophs on biogeochemical processes in the Antarctic. The project will directly support a graduate student, will positively impact the NSF REU program at the College of Marine and Earth Studies, and will include students from the nation?s oldest historical minority college. The results will be featured during weekly tours of Lewes facilities (about 1000 visitors per year) and during Coast Day, an annual open-house that attracts about 10,000 visitors.", "east": -64.040665, "geometry": "POINT(-64.0601655 -64.797665)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -64.77966, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Cottrell, Matthew; David, Kirchman", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -64.81567, "title": "Photoheterotrophic Microbes in the West Antarctic Peninsula Marine Ecosystem", "uid": "p0000473", "west": -64.079666}, {"awards": "0739698 Doran, Peter; 0739681 Murray, Alison", "bounds_geometry": "POINT(161.931 -77.3885)", "dataset_titles": "Geochemistry and Microbiology of the Extreme Aquatic Environment in Lake Vida, East Antarctica", "datasets": [{"dataset_uid": "600080", "doi": "10.15784/600080", "keywords": "Antarctica; Biota; Carbon-14; Chemistry:fluid; Chemistry:Fluid; Chemistry:ice; Chemistry:Ice; Dry Valleys; Geochronology; Ice Core Records; Lake Vida; Microbiology", "people": "Murray, Alison", "repository": "USAP-DC", "science_program": null, "title": "Geochemistry and Microbiology of the Extreme Aquatic Environment in Lake Vida, East Antarctica", "url": "https://www.usap-dc.org/view/dataset/600080"}], "date_created": "Thu, 12 Dec 2013 00:00:00 GMT", "description": "Lake Vida is the largest lake of the McMurdo Dry Valleys, with an approximately 20 m ice cover overlaying a brine of unknown depth with at least 7 times seawater salinity and temperatures below -10 degrees C year-round. Samples of brine collected from ice above the main water body contain 1) the highest nitrous oxide levels of any natural water body on Earth, 2) unusual geochemistry including anomalously high ammonia and iron concentrations, 3) high microbial counts with an unusual proportion (99%) of ultramicrobacteria. The microbial community is unique even compared to other Dry Valley Lakes. The research proposes to enter, for the first time the main brine body below the thick ice of Lake Vida and perform in situ measurements, collect samples of the brine column, and collect sediment cores from the lake bottom for detailed geochemical and microbiological analyses. The results will allow the characterization of present and past life in the lake, assessment of modern and past sedimentary processes, and determination of the lake\u0027s history. The research will be conducted by a multidisciplinary team that will uncover the biogeochemical processes associated with a non-photosynthetic microbial community isolated for a significant period of time. This research will address diversity, adaptive mechanisms and evolutionary processes in the context of the physical evolution of the environment of Lake Vida. Results will be widely disseminated through publications, presentations at national and international meetings, through the Subglacial Antarctic Lake Exploration (SALE) web site and the McMurdo LTER web site. The research will support three graduate students and three undergraduate research assistants. The results will be incorporated into a new undergraduate biogeosciences course at the University of Illinois at Chicago which has an extremely diverse student body, dominated by minorities.", "east": 161.931, "geometry": "POINT(161.931 -77.3885)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -77.3885, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Murray, Alison; Doran, Peter", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -77.3885, "title": "Collaborative Research: Geochemistry and Microbiology of the Extreme Aquatic Environment in Lake Vida, East Antarctica", "uid": "p0000485", "west": 161.931}, {"awards": "0739648 Cary, Stephen", "bounds_geometry": "POINT(163 -77.5)", "dataset_titles": "Biogeochemistry of Cyanobactrial Mats and Hyporheic Zone Microbes in McMurdo Dry Valley Glacial Meltwater Streams", "datasets": [{"dataset_uid": "600079", "doi": "10.15784/600079", "keywords": "Antarctica; Biota; Cell Counts; Dry Valleys; Microbiology", "people": "Cary, S. Craig", "repository": "USAP-DC", "science_program": null, "title": "Biogeochemistry of Cyanobactrial Mats and Hyporheic Zone Microbes in McMurdo Dry Valley Glacial Meltwater Streams", "url": "https://www.usap-dc.org/view/dataset/600079"}], "date_created": "Tue, 10 Dec 2013 00:00:00 GMT", "description": "The glacial streams of the McMurdo Dry Valleys have extensive cyanobacterial mats that are a probable source of fixed C and N to the Valleys. The research will examine the interplay between the microbial mats in the ephemeral glacial streams and the microbiota of the hyporheic soils (wetted soil zone) underlying and adjacent to those mats. It is hypothesized that the mats are important sources of organic carbon and fixed nitrogen for the soil communities of the hyporheic zone, and release dissolved organic carbon (DOC) and nitrogen (DON) that serves the entire Dry Valley ecosystem. Field efforts will entail both observational and experimental components. Direct comparisons will be made between the mats and microbial populations underlying naturally rehydrated and desiccated mat areas, and between mat areas in the melt streams of the Adams and Miers Glaciers in Miers Valley. Both physiological and phylogenetic indices of the soil microbiota will be examined. Observations will include estimates of rates of mat carbon and nitrogen fixation, soil respiration and leucine and thymidine uptake (as measures of protein \u0026 DNA synthesis, respectively) by soil bacteria, bacterial densities and their molecular ecology. Experimental manipulations will include experimental re-wetting of soils and observations of the time course of response of the microbial community. The research will integrate modern molecular genetic approaches (ARISA-DNA fingerprinting and ultra deep 16S rDNA microbial phylogenetic analysis) with geochemistry to study the diversity, ecology, and function of microbial communities that thrive in these extreme environments. The broader impacts of the project include research and educational opportunities for graduate students and a postdoctoral associate. The P.I.s will involve undergraduates as work-study students and in REU programs, and will participate in educational and outreach programs.", "east": 163.0, "geometry": "POINT(163 -77.5)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -77.5, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Cary, Stephen", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -77.5, "title": "Collaborative Research: Biogeochemistry of Cyanobactrial Mats and Hyporheic Zone Microbes in McMurdo Dry Valley Glacial Meltwater Streams", "uid": "p0000476", "west": 163.0}, {"awards": "0838850 Gooseff, Michael", "bounds_geometry": "POLYGON((162.32 -77.62,162.418 -77.62,162.516 -77.62,162.614 -77.62,162.712 -77.62,162.81 -77.62,162.90800000000002 -77.62,163.006 -77.62,163.104 -77.62,163.202 -77.62,163.3 -77.62,163.3 -77.631,163.3 -77.64200000000001,163.3 -77.653,163.3 -77.664,163.3 -77.67500000000001,163.3 -77.686,163.3 -77.697,163.3 -77.708,163.3 -77.71900000000001,163.3 -77.73,163.202 -77.73,163.104 -77.73,163.006 -77.73,162.90800000000002 -77.73,162.81 -77.73,162.712 -77.73,162.614 -77.73,162.516 -77.73,162.418 -77.73,162.32 -77.73,162.32 -77.71900000000001,162.32 -77.708,162.32 -77.697,162.32 -77.686,162.32 -77.67500000000001,162.32 -77.664,162.32 -77.653,162.32 -77.64200000000001,162.32 -77.631,162.32 -77.62))", "dataset_titles": "The Role of Snow Patches on the Spatial Distribution of Soil Microbial Communities and Biogeochemical Cycling in the Antarctic Dry Valleys", "datasets": [{"dataset_uid": "600100", "doi": "10.15784/600100", "keywords": "Antarctica; Critical Zone; Mps-1 Water Potential Sensor; Physical Properties; Soil Moisture; Soil Temperature", "people": "Gooseff, Michael N.", "repository": "USAP-DC", "science_program": null, "title": "The Role of Snow Patches on the Spatial Distribution of Soil Microbial Communities and Biogeochemical Cycling in the Antarctic Dry Valleys", "url": "https://www.usap-dc.org/view/dataset/600100"}], "date_created": "Tue, 26 Nov 2013 00:00:00 GMT", "description": "This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).\u003cbr/\u003e\u003cbr/\u003eTwo models have been proposed to describe controls over microbial biogeography. One model proposes that microbes are ubiquitously distributed across the global environment, and that environmental conditions select for taxa physiologically adapted to local physical conditions. An alternative model predicts that dispersal is the important limitation to the distribution of microorganisms and that spatial heterogeneity of microbial communities is a result of both dispersal and local environmental limitations. According to both models, spatial heterogeneity of microbial communities may be especially pronounced in extreme ecosystems where the environmental selection for organisms with suitable physiology is most strongly manifest. We propose that Antarctic terrestrial environments are ideal places to examine microbial biogeography for 3 reasons: 1) the pristine nature and remoteness of Antarctica minimizes the prevalence of exotic species dispersed through human vectors; 2) the extreme conditions of Antarctic environments provide a strong environmental filter which limits the establishment of non-indigenous taxa; and 3) extreme heterogeneity in the terrestrial environment provides natural gradients of soil conditions (temperature, water and nutrient availability). In the proposed research we will investigate the influence of snow on the composition and spatial distribution of soil microbial communities and linked biogeochemical cycling in the McMurdo Dry Valleys. We will conduct fieldwork at the landscape scale (repeated remote sensing to characterize snow distribution), at the valley and patch scales (quantify snow patch ablation, microbial communities and biogeochemical cycling in subnivian soils). We hypothesize that snow patches play an important role in structuring the spatial distribution of soil microbial communities and their associated ecosystem functioning because of the physical and hydrological influences that snow patches have on the soil environment. The research will contribute to greater public awareness of the importance of polar research to fundamental questions of biology, ecology and hydrology through direct linkages with International Antarctic Institute public outreach activities, including dissemination of web-based learning units on environmental science and microbiology, targeted as resources for secondary and post-secondary educators. Three graduate students, one postdoctoral scholar and multiple undergraduates will participate in the research activities.", "east": 163.3, "geometry": "POINT(162.81 -77.675)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -77.62, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Gooseff, Michael N.", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -77.73, "title": "Collaborative Research: The Role of Snow Patches on the Spatial Distribution of Soil Microbial Communities and Biogeochemical Cycling in the Antarctic Dry Valleys", "uid": "p0000489", "west": 162.32}, {"awards": "0739783 Junge, Karen", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "Metabolic Activities and Gene Expression of Marine Psychrophiles in Cold Ice", "datasets": [{"dataset_uid": "600083", "doi": "10.15784/600083", "keywords": "Antarctica; Biota; Microbiology; Oceans; Sea Ice; Southern Ocean", "people": "Junge, Karen", "repository": "USAP-DC", "science_program": null, "title": "Metabolic Activities and Gene Expression of Marine Psychrophiles in Cold Ice", "url": "https://www.usap-dc.org/view/dataset/600083"}], "date_created": "Wed, 25 Sep 2013 00:00:00 GMT", "description": "The mechanisms enabling bacteria to be metabolically active at very low temperatures are of considerable importance to polar microbial ecology, astrobiology, climate and cryopreservation. This research program has two main objectives. The first is to investigate metabolic activities and gene expression of polar marine psychrophilic bacteria when confronted with freezing conditions at temperatures above the eutectic of seawater (\u003c54C) to unveil cold adaptation mechanisms with relevance to wintertime sea-ice ecology. The second objective is to discern if psychrophilic processes of leucine incorporation into proteins, shown to occur to -196C, amount to metabolic activity providing for the survival of cells or are merely biochemical reactions still possible in flash-frozen samples without any effect on survival. We will examine extracellular and intracellular processes of psychrophilic activity above and below the eutectic by (i) determining the temperature range of metabolic activities such as DNA synthesis, carbon utilization, respiration and ATP generation using radioactive tracer technology, including a control at liquid helium temperature (-268.9C), (ii) analyzing gene expression in ice using whole genome and microarray analyses and iii) examining the role of exopolymeric substances (EPS) and ice micro-physics for the observed activity using an in-situ microscopy technique. Results of the proposed research can be expected to aid in the determination of cellular and genetic strategies that allow cells to maintain activity at extremely low temperatures within an icy matrix and/or to resume activity again when more growth-permissive conditions are encountered. The research is an interdisciplinary collaboration involving three different institutions with participants in Oceanography, Genomics, and Geophysical Sciences. The proposed activity will support the beginning professional career of a female researcher and will serve as the basis for several undergraduate student laboratory projects.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Junge, Karen", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -90.0, "title": "Metabolic Activities and Gene Expression of Marine Psychrophiles in Cold Ice", "uid": "p0000673", "west": -180.0}, {"awards": "0738658 Price, P. Buford", "bounds_geometry": "POINT(112.1125 -79.4638)", "dataset_titles": "Access to data; data from one of three optical logs we made at WAIS Divide; WAIS Divide Laser Dust Logger Data", "datasets": [{"dataset_uid": "609540", "doi": "10.7265/N5C53HSG", "keywords": "Antarctica; Atmosphere; Chemistry:ice; Chemistry:Ice; Dust; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Ice Core Records; Laser Dust Logger; WAIS Divide Ice Core", "people": "Bay, Ryan", "repository": "USAP-DC", "science_program": "WAIS Divide Ice Core", "title": "WAIS Divide Laser Dust Logger Data", "url": "https://www.usap-dc.org/view/dataset/609540"}, {"dataset_uid": "000188", "doi": "", "keywords": null, "people": null, "repository": "USAP-DC", "science_program": null, "title": "data from one of three optical logs we made at WAIS Divide", "url": "http://icecube.berkeley.edu/~bay/wdc/"}, {"dataset_uid": "001349", "doi": "", "keywords": null, "people": null, "repository": "PI website", "science_program": null, "title": "Access to data", "url": "http://icecube.berkeley.edu/~bay/wdc/"}], "date_created": "Tue, 19 Jun 2012 00:00:00 GMT", "description": "This award supports a project to use two new scanning fluorimeters to map microbial concentrations vs depth in the WAIS Divide ice core as portions of it become available at NICL, and selected portions of the GISP2 ice core for inter-hemispheric comparison. Ground-truth calibrations with microbes in ice show that the instruments are sensitive to a single cell and can scan the full length of a 1-meter core at 300-micron intervals in two minutes. The goals of these studies will be to exploit the discovery that microbes are transported onto ice, in clumps, several times per year and that at rare intervals (not periodically) of ~104 years, a much higher flux, sometimes lasting \u003e1 decade, reaches the ice. From variations ranging from seasonal to millennial to glacial scale in the arrival time distribution of phototrophs, methanogens, and total microbes in the Antarctic and Arctic ice, the investigators will attempt to determine oceanic and terrestrial sources of these microbes and will look for correlations of microbial bursts with dust concentration and temperature proxies. In addition the project will follow up on the discovery that the rare instances of very high microbial flux account for some of the\"gas artifacts\" in ice cores - isolated spikes of excess CH4 and N2O that have been discarded by others in previous climate studies. The intellectual merit of this project is that it will exploit scanning fluorimetry of microbes as a powerful new tool for studies ranging from meteorology to climatology to biology, especially when combined with mapping of dust, gases, and major element chemistry in ice cores. In 2010-11 the WAIS Divide borehole will be logged with the latest version of the dust logger. The log will provide mm-scale depth resolution of dust concentration and of volcanic ash layers down the entire depth of the borehole. The locations of ash layers in the ice will be determined and chemical analyses of the ash will be analyzed in order to determine provenance. By comparing data from the WAIS Divide borehole with data from other boreholes and with chemical data (obtained by others) on volcanic layers, the researchers will examine the relationship between the timing of volcanic eruptions and abrupt climate change. Results from this project with the scanning fluorimeters and the dust logger could have applications to planetary missions, borehole oceanography, limnology, meteorology, climate, volcanology, and ancient life in ice. A deeper understanding of the causes of abrupt climate change, including a causal relationship with volcanic explosivity, would enable a better understanding of the adverse effects on climate. The broader impact of the project is that it will provide training to students and post-docs from the U. S. and other countries.", "east": 112.1125, "geometry": "POINT(112.1125 -79.4638)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e RECORDERS/LOGGERS \u003e OPTICAL DUST LOGGERS; IN SITU/LABORATORY INSTRUMENTS \u003e RECORDERS/LOGGERS \u003e OPTICAL DUST LOGGERS; IN SITU/LABORATORY INSTRUMENTS \u003e CHEMICAL METERS/ANALYZERS \u003e FLUOROMETERS", "is_usap_dc": true, "keywords": "Dust Loggers; Dust Concentration; Ice Core; West Antarctic Ice Sheet; LABORATORY; Microbial; Fluorimetry; GROUND-BASED OBSERVATIONS; Meteorology; Climatologymeteorologyatmosphere; Ice", "locations": "West Antarctic Ice Sheet", "north": -79.4638, "nsf_funding_programs": "Antarctic Glaciology", "paleo_time": null, "persons": "Bay, Ryan; Price, Buford; Souney, Joseph Jr.", "platforms": "LAND-BASED PLATFORMS \u003e PERMANENT LAND SITES \u003e GROUND-BASED OBSERVATIONS; OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repo": "USAP-DC", "repositories": "PI website; USAP-DC", "science_programs": "WAIS Divide Ice Core", "south": -79.4638, "title": "Climatology, Meteorology, and Microbial Metabolism in Ice with Dust Loggers and Fluorimetry", "uid": "p0000009", "west": 112.1125}, {"awards": "0230499 Kieber, David", "bounds_geometry": "POLYGON((-179.99998 -43.58056,-143.999984 -43.58056,-107.999988 -43.58056,-71.999992 -43.58056,-35.999996 -43.58056,0 -43.58056,35.999996 -43.58056,71.999992 -43.58056,107.999988 -43.58056,143.999984 -43.58056,179.99998 -43.58056,179.99998 -46.971468,179.99998 -50.362376,179.99998 -53.753284,179.99998 -57.144192,179.99998 -60.5351,179.99998 -63.926008,179.99998 -67.316916,179.99998 -70.707824,179.99998 -74.098732,179.99998 -77.48964,143.999984 -77.48964,107.999988 -77.48964,71.999992 -77.48964,35.999996 -77.48964,0 -77.48964,-35.999996 -77.48964,-71.999992 -77.48964,-107.999988 -77.48964,-143.999984 -77.48964,-179.99998 -77.48964,-179.99998 -74.098732,-179.99998 -70.707824,-179.99998 -67.316916,-179.99998 -63.926008,-179.99998 -60.5351,-179.99998 -57.144192,-179.99998 -53.753284,-179.99998 -50.362376,-179.99998 -46.971468,-179.99998 -43.58056))", "dataset_titles": "Expedition Data", "datasets": [{"dataset_uid": "001616", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/NBP0409"}], "date_created": "Tue, 17 Jan 2012 00:00:00 GMT", "description": "Areas of the Southern Ocean have spectacular blooms of phytoplankton during the austral spring and early summer. One of the dominant phytoplankton species, the haptophyte Phaeocystis antarctica, is a prolific producer of the organic sulfur compound dimethylsulfoniopropionate (DMSP) and Phaeocystis blooms are associated with some of the world\u0027s highest concentrations of DMSP and its volatile degradation product, dimethylsulfide (DMS). Sulfur, in the form of DMS, is transferred from the oceans to the atmosphere and can affect the chemistry of precipitation and influence cloud properties and possibly climate. DMSP and DMS are also quantitatively significant components of the carbon, sulfur and energy flows in many marine food webs, although very little information is available on these processes in high latitude systems. \u003cbr/\u003e\u003cbr/\u003eThis project will study how solar radiation and iron cycling affect DMSP and DMS production by phytoplankton, and the subsequent utilization of these labile forms of organic matter by the microbial food web. Four interrelated hypotheses will be tested in field-based experiments and in situ observations: 1) solar radiation, including enhanced UV-B due to seasonal ozone depletion, plays an important role in determining the net ecosystem production of DMS in the Ross Sea; 2) development of shallow mixed layers promotes the accumulation of DMS in surface waters, because of enhanced exposure of plankton communities to high doses of solar radiation; 3) DMSP production and turnover represent a significant part of the carbon and sulfur flux through polar food webs; 4) bloom development and resulting nutrient depletion (e.g., iron) will result in high production rates of DMSP and high DMS concentrations and atmospheric fluxes. Results from this study will greatly improve understanding of the underlying mechanisms controlling DMSP and DMS concentrations in polar waters, thereby improving our ability to predict DMS fluxes to the atmosphere from this important climatic region. \u003cbr/\u003e\u003cbr/\u003eBoth Drs. Kieber and Kiene actively engage high school, undergraduate and graduate students in their research and are involved in formal programs that target underrepresented groups (NSF-REU and the American Chemical Society-SEED). This project will continue this type of educational outreach. The PIs also teach undergraduate and graduate courses and incorporation of research experiences into their classes will enrich student learning experiences.", "east": 179.99998, "geometry": "POINT(0 -89.999)", "instruments": "EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS", "is_usap_dc": false, "keywords": "R/V NBP", "locations": null, "north": -43.58056, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Kiene, Ronald", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repo": "R2R", "repositories": "R2R", "science_programs": null, "south": -77.48964, "title": "Collaborative Research: Impact of Solar Radiation and Nutrients on Biogeochemical Cycling of DMSP and DMS in the Ross Sea, Antarctica", "uid": "p0000582", "west": -179.99998}, {"awards": "0230497 Kiene, Ronald", "bounds_geometry": null, "dataset_titles": "Expedition Data; Expedition data of NBP0409", "datasets": [{"dataset_uid": "001616", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/NBP0409"}, {"dataset_uid": "002640", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of NBP0409", "url": "https://www.rvdata.us/search/cruise/NBP0409"}], "date_created": "Tue, 17 Jan 2012 00:00:00 GMT", "description": "Areas of the Southern Ocean have spectacular blooms of phytoplankton during the austral spring and early summer. One of the dominant phytoplankton species, the haptophyte Phaeocystis antarctica, is a prolific producer of the organic sulfur compound dimethylsulfoniopropionate (DMSP) and Phaeocystis blooms are associated with some of the world\u0027s highest concentrations of DMSP and its volatile degradation product, dimethylsulfide (DMS). Sulfur, in the form of DMS, is transferred from the oceans to the atmosphere and can affect the chemistry of precipitation and influence cloud properties and possibly climate. DMSP and DMS are also quantitatively significant components of the carbon, sulfur and energy flows in many marine food webs, although very little information is available on these processes in high latitude systems. \u003cbr/\u003e\u003cbr/\u003eThis project will study how solar radiation and iron cycling affect DMSP and DMS production by phytoplankton, and the subsequent utilization of these labile forms of organic matter by the microbial food web. Four interrelated hypotheses will be tested in field-based experiments and in situ observations: 1) solar radiation, including enhanced UV-B due to seasonal ozone depletion, plays an important role in determining the net ecosystem production of DMS in the Ross Sea; 2) development of shallow mixed layers promotes the accumulation of DMS in surface waters, because of enhanced exposure of plankton communities to high doses of solar radiation; 3) DMSP production and turnover represent a significant part of the carbon and sulfur flux through polar food webs; 4) bloom development and resulting nutrient depletion (e.g., iron) will result in high production rates of DMSP and high DMS concentrations and atmospheric fluxes. Results from this study will greatly improve understanding of the underlying mechanisms controlling DMSP and DMS concentrations in polar waters, thereby improving our ability to predict DMS fluxes to the atmosphere from this important climatic region. \u003cbr/\u003e\u003cbr/\u003eBoth Drs. Kieber and Kiene actively engage high school, undergraduate and graduate students in their research and are involved in formal programs that target underrepresented groups (NSF-REU and the American Chemical Society-SEED). This project will continue this type of educational outreach. The PIs also teach undergraduate and graduate courses and incorporation of research experiences into their classes will enrich student learning experiences.", "east": null, "geometry": null, "instruments": "EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS", "is_usap_dc": false, "keywords": "R/V NBP", "locations": null, "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Kiene, Ronald", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repo": "R2R", "repositories": "R2R", "science_programs": null, "south": null, "title": "Collaborative Research: Impact of Solar Radiation and Nutrients on Biogeochemical Cycling of DMSP and DMS in the Ross Sea, Antarctica", "uid": "p0000832", "west": null}, {"awards": "0836144 Yager, Patricia; 0836061 Dennett, Mark; 0836112 Smith, Walker", "bounds_geometry": "POLYGON((100 -69,107 -69,114 -69,121 -69,128 -69,135 -69,142 -69,149 -69,156 -69,163 -69,170 -69,170 -70,170 -71,170 -72,170 -73,170 -74,170 -75,170 -76,170 -77,170 -78,170 -79,163 -79,156 -79,149 -79,142 -79,135 -79,128 -79,121 -79,114 -79,107 -79,100 -79,100 -78,100 -77,100 -76,100 -75,100 -74,100 -73,100 -72,100 -71,100 -70,100 -69))", "dataset_titles": "Amundsen Sea Polynya International Research Expedition (ASPIRE) data; Controls on Climate-Active Gases by Amundsen Sea Ice Biota", "datasets": [{"dataset_uid": "600092", "doi": "10.15784/600092", "keywords": "Amundsen Sea; Antarctica; Chemistry:fluid; Chemistry:Fluid; CTD Data; Oceans; Oden; Oden2008; Sea Ice; Sea Surface; Southern Ocean", "people": "Smith, Walker", "repository": "USAP-DC", "science_program": null, "title": "Controls on Climate-Active Gases by Amundsen Sea Ice Biota", "url": "https://www.usap-dc.org/view/dataset/600092"}, {"dataset_uid": "000146", "doi": "", "keywords": null, "people": null, "repository": "BCO-DMO", "science_program": null, "title": "Amundsen Sea Polynya International Research Expedition (ASPIRE) data", "url": "https://www.bco-dmo.org/project/2132"}, {"dataset_uid": "600091", "doi": "10.15784/600091", "keywords": "Amundsen Sea; Antarctica; Biota; Oceans; Oden; Oden2008; Plankton; Sea Ice; Southern Ocean", "people": "Dennett, Mark", "repository": "USAP-DC", "science_program": null, "title": "Controls on Climate-Active Gases by Amundsen Sea Ice Biota", "url": "https://www.usap-dc.org/view/dataset/600091"}], "date_created": "Sun, 24 Apr 2011 00:00:00 GMT", "description": "Convincing evidence now confirms that polar regions are changing rapidly in response to human activities. Changes in sea ice extent and thickness will have profound implications for productivity, food webs and carbon fluxes at high latitudes, since sea ice biota are a significant source of biogenic matter for the ecosystem. While sea ice is often thought to be a barrier to gas exchange between the ocean and the atmosphere, it more likely functions as a source or sink for climate-active gases such as carbon dioxide and ozone-depleting organohalogens, due in part to activities of microbes embedded in the sea ice matrix. This project brings together experienced US and Swedish investigators to examine the controls by sea-ice biota on the production and degradation of key climate-active gases in the Pacific sector of the Southern Ocean. We hypothesize that 1) the physical properties of the sea-ice environment will determine the community structure and activities of the sea ice biota; 2) the productivity, biomass, physiological state and species composition of ice algae will determine the production of specific classes of organic carbon, including organohalogens; 3) heterotrophic co-metabolism within the ice will break down these compounds to some extent, depending on the microbial community structure and productivity, and 4) the sea ice to atmosphere fluxes of CO2 and organohalogens will be inversely related. This project will build close scientific collaborations between US and Swedish researchers and also train young scientists, including members of underrepresented groups. Dissemination of results will include the scientific literature, and public outreach venues including interactions with a PolarTrec teacher.", "east": 170.0, "geometry": "POINT(135 -74)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -69.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Smith, Walker; Yager, Patricia; Dennett, Mark", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "BCO-DMO; USAP-DC", "science_programs": null, "south": -79.0, "title": "Collaborative Research: Controls on climate-active gases by Amundsen Sea ice biota", "uid": "p0000137", "west": 100.0}, {"awards": "0636730 Vernet, Maria", "bounds_geometry": "POLYGON((-55 -52,-53.5 -52,-52 -52,-50.5 -52,-49 -52,-47.5 -52,-46 -52,-44.5 -52,-43 -52,-41.5 -52,-40 -52,-40 -53.3,-40 -54.6,-40 -55.9,-40 -57.2,-40 -58.5,-40 -59.8,-40 -61.1,-40 -62.4,-40 -63.7,-40 -65,-41.5 -65,-43 -65,-44.5 -65,-46 -65,-47.5 -65,-49 -65,-50.5 -65,-52 -65,-53.5 -65,-55 -65,-55 -63.7,-55 -62.4,-55 -61.1,-55 -59.8,-55 -58.5,-55 -57.2,-55 -55.9,-55 -54.6,-55 -53.3,-55 -52))", "dataset_titles": "Free-drifting Icebergs as Proliferating Dispersion Sites of Iron Enrichment, Organic Carbon Production and Export in the Southern Ocean", "datasets": [{"dataset_uid": "600068", "doi": "10.15784/600068", "keywords": "Antarctica; Biota; Chemistry:fluid; Chemistry:Fluid; Geochemistry; NBP0902; Oceans; Physical Oceanography; Sea Surface; Southern Ocean; Weddell Sea", "people": "Vernet, Maria", "repository": "USAP-DC", "science_program": null, "title": "Free-drifting Icebergs as Proliferating Dispersion Sites of Iron Enrichment, Organic Carbon Production and Export in the Southern Ocean", "url": "https://www.usap-dc.org/view/dataset/600068"}], "date_created": "Mon, 22 Nov 2010 00:00:00 GMT", "description": "Atmospheric warming has been associated with retreating glaciers, disintegrating ice shelves, and the increasing prevalence of icebergs in the Southern Ocean over the last decade. Our preliminary study of two icebergs in the NW Weddell Sea, an area of high iceberg concentration, showed significant delivery of terrestrial material accompanied by significant enhancement of phytoplankton and zooplankton/micronekton abundance, and primary production surrounding the icebergs. We hypothesize that nutrient enrichment by free-drifting icebergs will increase primary production and sedimentation of organic carbon, thus increasing the draw-down and sequestration of CO2 in the Southern Ocean and impacting the global carbon cycle. Our research addresses the following questions:1) What is the relationship between the physical dynamics of free-drifting icebergs and the Fe and nutrient distributions of the surrounding water column? 2) What is the relationship between Fe and nutrient distributions associated with free-drifting icebergs and the organic carbon dynamics of the ice-attached and surrounding pelagic communities (microbes, zooplankton, micronekton)? 3) What is impact on the export flux of particulate organic carbon from the mixed layer? An interdisciplinary approach is proposed to examine iceberg structure and dynamics, biogeochemical processes, and carbon cycling that includes measurement of trace element, nutrient and radionuclide distributions; organic carbon dynamics mediated by microbial, ice-attached and zooplankton communities; and particulate organic carbon export fluxes. Results from this project will further our understanding of the relationship between climate change and carbon sequestration in the Southern Ocean. Our findings will be incorporated into the Antarctic Research division of the Ocean Exploration Center (OEC) as part of the SIOExplorer: Digital Library Project. The OEC allows users to access content, which is classified to one of four levels: entry (grade K-6), student (grade 6-12), college, and research. Graduate students, undergraduates, teachers, and volunteers are important participants in the proposed field and laboratory work. For the K-12 level, a professional writer of children\u0027s books will participate in cruises to produce an account of the expedition and a daily interactive website.", "east": -40.0, "geometry": "POINT(-47.5 -58.5)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -52.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Vernet, Maria", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -65.0, "title": "Collaborative Reseach: Free-drifting Icebergs as Proliferating Dispersion Sites of Iron Enrichment, Organic Carbon Production and Export in the Southern Ocean.", "uid": "p0000532", "west": -55.0}, {"awards": "0636543 Murray, Alison; 0636319 Shaw, Timothy; 0636723 Helly, John; 0636440 Long, David", "bounds_geometry": "POLYGON((-55 -52,-53.5 -52,-52 -52,-50.5 -52,-49 -52,-47.5 -52,-46 -52,-44.5 -52,-43 -52,-41.5 -52,-40 -52,-40 -53.3,-40 -54.6,-40 -55.9,-40 -57.2,-40 -58.5,-40 -59.8,-40 -61.1,-40 -62.4,-40 -63.7,-40 -65,-41.5 -65,-43 -65,-44.5 -65,-46 -65,-47.5 -65,-49 -65,-50.5 -65,-52 -65,-53.5 -65,-55 -65,-55 -63.7,-55 -62.4,-55 -61.1,-55 -59.8,-55 -58.5,-55 -57.2,-55 -55.9,-55 -54.6,-55 -53.3,-55 -52))", "dataset_titles": "Antarctic Iceberg Tracking Database; Free-Drifting Icebergs as Proliferating Dispersion Sites of Iron Enrichment, Organic Carbon Production and Export in the Southern Ocean; Free Drifting Icebergs as Proliferation Sites of Iron Enrichment, Organic Carbon Production and Export in the Southern Ocean", "datasets": [{"dataset_uid": "000134", "doi": "", "keywords": null, "people": null, "repository": "PI website", "science_program": null, "title": "Antarctic Iceberg Tracking Database", "url": "http://www.scp.byu.edu/data/iceberg/database1.html"}, {"dataset_uid": "600067", "doi": "10.15784/600067", "keywords": "Antarctica; NBP0902; Oceans; Physical Oceanography; Southern Ocean; Weddell Sea", "people": "Helly, John", "repository": "USAP-DC", "science_program": null, "title": "Free-Drifting Icebergs as Proliferating Dispersion Sites of Iron Enrichment, Organic Carbon Production and Export in the Southern Ocean", "url": "https://www.usap-dc.org/view/dataset/600067"}, {"dataset_uid": "600065", "doi": "10.15784/600065", "keywords": "Biota; Geochemistry; NBP0902; Oceans; Physical Oceanography; Sea Ice; Southern Ocean; Weddell Sea", "people": "Murray, Alison", "repository": "USAP-DC", "science_program": null, "title": "Free-Drifting Icebergs as Proliferating Dispersion Sites of Iron Enrichment, Organic Carbon Production and Export in the Southern Ocean", "url": "https://www.usap-dc.org/view/dataset/600065"}, {"dataset_uid": "600064", "doi": "10.15784/600064", "keywords": "Biota; Chemistry:fluid; Chemistry:Fluid; Geochemistry; Oceans; Sea Ice; Sea Surface; Southern Ocean; Weddell Sea", "people": "Twining, Benjamin; Shaw, Tim", "repository": "USAP-DC", "science_program": null, "title": "Free Drifting Icebergs as Proliferation Sites of Iron Enrichment, Organic Carbon Production and Export in the Southern Ocean", "url": "https://www.usap-dc.org/view/dataset/600064"}], "date_created": "Mon, 22 Nov 2010 00:00:00 GMT", "description": "Atmospheric warming has been associated with retreating glaciers, disintegrating ice shelves, and the increasing prevalence of icebergs in the Southern Ocean over the last decade. Our preliminary study of two icebergs in the NW Weddell Sea, an area of high iceberg concentration, showed significant delivery of terrestrial material accompanied by significant enhancement of phytoplankton and zooplankton/micronekton abundance, and primary production surrounding the icebergs. We hypothesize that nutrient enrichment by free-drifting icebergs will increase primary production and sedimentation of organic carbon, thus increasing the draw-down and sequestration of CO2 in the Southern Ocean and impacting the global carbon cycle. Our research addresses the following questions:1) What is the relationship between the physical dynamics of free-drifting icebergs and the Fe and nutrient distributions of the surrounding water column? 2) What is the relationship between Fe and nutrient distributions associated with free-drifting icebergs and the organic carbon dynamics of the ice-attached and surrounding pelagic communities (microbes, zooplankton, micronekton)? 3) What is impact on the export flux of particulate organic carbon from the mixed layer? An interdisciplinary approach is proposed to examine iceberg structure and dynamics, biogeochemical processes, and carbon cycling that includes measurement of trace element, nutrient and radionuclide distributions; organic carbon dynamics mediated by microbial, ice-attached and zooplankton communities; and particulate organic carbon export fluxes. Results from this project will further our understanding of the relationship between climate change and carbon sequestration in the Southern Ocean. Our findings will be incorporated into the Antarctic Research division of the Ocean Exploration Center (OEC) as part of the SIOExplorer: Digital Library Project. The OEC allows users to access content, which is classified to one of four levels: entry (grade K-6), student (grade 6-12), college, and research. Graduate students, undergraduates, teachers, and volunteers are important participants in the proposed field and laboratory work. For the K-12 level, a professional writer of children\u0027s books will participate in cruises to produce an account of the expedition and a daily interactive website.", "east": -40.0, "geometry": "POINT(-47.5 -58.5)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -52.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Twining, Benjamin; Shaw, Tim; Long, David; Murray, Alison; Helly, John", "platforms": "Not provided", "repo": "PI website", "repositories": "PI website; USAP-DC", "science_programs": null, "south": -65.0, "title": "Collaborative Research: Free Drifting Icebergs as Proliferation Sites of Iron Enrichment, Organic Carbon Production and Export in the Southern Ocean", "uid": "p0000511", "west": -55.0}, {"awards": "0631494 Priscu, John; 0631659 Morgan-Kiss, Rachael", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "GenBank (NCBI) 18S rRNA genes: GU969060 to GU969102, rbcL genes: GU132860 to GU132939; McMurdo Dry Valleys Long-Term Ecological Research (MCM LTER) Program", "datasets": [{"dataset_uid": "000126", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "GenBank (NCBI) 18S rRNA genes: GU969060 to GU969102, rbcL genes: GU132860 to GU132939", "url": "http://www.ncbi.nlm.nih.gov/genbank/"}, {"dataset_uid": "000125", "doi": "", "keywords": null, "people": null, "repository": "LTER", "science_program": null, "title": "McMurdo Dry Valleys Long-Term Ecological Research (MCM LTER) Program", "url": "http://www.mcmlter.org/"}], "date_created": "Fri, 08 Oct 2010 00:00:00 GMT", "description": "Data collected on the permanently ice-covered lakes of the McMurdo Dry Valleys (MCM) during the late 1950\u0027s as part of the International Geophysical Year (IGY) showed that they were the only year round liquid water environments on the continent. Organisms in the lakes must possess novel physiological strategies that allow them to survive at low temperature and under extended darkness. Subsequent research has now shown that most organisms in the lakes are not just \"surviving the extremes\" but are actively feeding, growing and reproducing. However, nearly all research on the MCM lakes is restricted to the austral spring and summer when logistical support is provided. The unique aspects of physiological adaptation and metabolic function during the permanently cold and prolonged darkness of the Antarctic winter remain unknown. As part of the \"International Polar Year 2007-2008\" (IPY), the proposed research will study lakes within the Taylor Valley during the transition to polar night to test the overarching hypothesis that the onset of darkness induces a cascade of physiological changes that alters the functional role of autotrophic and heterotrophic microplankton within the lakes. This overarching theme will be addressed through an interdisciplinary study of selected biological components of the lake ecosystems using genomic and physiological tools to understand not only how individual organisms survive, but how they control ecosystem function during this seasonal transition. \u003cbr/\u003e\u003cbr/\u003eThis project is directly relevant to IPY objectives as it addresses a major identified theme (Adaptations to Life in Extreme Cold and Prolonged Darkness) with an international (UK, NZ),\u003cbr/\u003emultidisciplinary team. The research has substantial broader impacts, as it will add to the body of long-term data accumulated by the MCM LTER and MCM Microbial Observatory projects in a synergistic manner; and it will include three undergraduates, a graduate student and two young female investigators. The project is linked to a highly visible education, outreach and human diversity programs supported by the McMurdo LTER, and initiates new outreach programs, including the Passport to Knowledge program.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Morgan-Kiss, Rachael; Priscu, John; Mikucki, Jill", "platforms": "Not provided", "repo": "NCBI GenBank", "repositories": "LTER; NCBI GenBank", "science_programs": "LTER", "south": -90.0, "title": "Collaborative Research: IPY- Plankton Dynamics in the McMurdo Dry Valley Lakes During the Transition to Polar Night", "uid": "p0000525", "west": -180.0}, {"awards": "0537143 Blanchette, Robert", "bounds_geometry": "POLYGON((-69 -60,-68.3 -60,-67.6 -60,-66.9 -60,-66.2 -60,-65.5 -60,-64.8 -60,-64.1 -60,-63.4 -60,-62.7 -60,-62 -60,-62 -61,-62 -62,-62 -63,-62 -64,-62 -65,-62 -66,-62 -67,-62 -68,-62 -69,-62 -70,-62.7 -70,-63.4 -70,-64.1 -70,-64.8 -70,-65.5 -70,-66.2 -70,-66.9 -70,-67.6 -70,-68.3 -70,-69 -70,-69 -69,-69 -68,-69 -67,-69 -66,-69 -65,-69 -64,-69 -63,-69 -62,-69 -61,-69 -60))", "dataset_titles": "(Arenz et al. 2006) DQ317323, DQ317324, DQ317325, DQ317326, DQ317327, DQ317328, DQ317329, DQ317330, DQ317331, DQ317332, DQ317333, DQ317334, DQ317335, DQ317336, DQ317337, DQ317338, DQ317339, DQ317340, DQ317341, DQ317342, DQ317343, DQ317344, DQ317345, DQ317346, DQ317347, DQ317348, DQ317349, DQ317350, DQ317351, DQ317352, DQ317353, DQ317354, DQ317355, DQ317356, DQ317357, DQ317358, DQ317359, DQ317360, DQ317361, DQ317362, DQ317363, DQ317364, DQ317365, DQ317366, DQ317367, DQ317368, DQ317369, DQ317370, DQ317371, DQ317372, DQ317373, DQ317374, DQ317375, DQ317376, DQ317377, DQ317378, DQ317379, DQ317380, DQ317381, DQ317382, DQ317383, DQ317384, DQ317385, DQ317386, DQ317387, DQ317388, DQ317389 (Arenz and Blanchette 2009) FJ235934, FJ235935, FJ235936, FJ235937, FJ235938, FJ235939, FJ235940, FJ235941, FJ235942, FJ235943, FJ235944, FJ235945, FJ235946, FJ235947, FJ235948, FJ235949, FJ235950, FJ235951, FJ235952, FJ235953, FJ235954, FJ235955, FJ235956, FJ235957, FJ235958, FJ235959, FJ235960, FJ235961, FJ235962, FJ235963, FJ235964, FJ235965, FJ235966, FJ235967, FJ235968, FJ235969, FJ235970, FJ235971, FJ235972, FJ235973, FJ235974, FJ235975, FJ235976, FJ235977, FJ235978, FJ235979, FJ235980, FJ235981, FJ235982, FJ235983, FJ235984, FJ235985, FJ235986, FJ235987, FJ235988, FJ235989, FJ235990, FJ235991, FJ235992, FJ235993, FJ235994, FJ235995, FJ235996, FJ235997, FJ235998, FJ235999, FJ236000, FJ236001, FJ236002, FJ236003, FJ236004, FJ236005, FJ236006, FJ236007, FJ236008, FJ236009, FJ236010, FJ236011, FJ236012, FJ236013, FJ236014 (Blanchette et al. 2010) GU212367, GU212368, GU212369, GU212370, GU212371, GU212372, GU212373, GU212374, GU212375, GU212376, GU212377, GU212378, GU212379, GU212380, GU212381, GU212382, GU212383, GU212384, GU212385, GU212386, GU212387, GU212388, GU212389, GU212390, GU212391, GU212392, GU212393, GU212394, GU212395, GU212396, GU212397, GU212398, GU212399, GU212400, GU212401, GU212402, GU212403, GU212404, GU212405, GU212406, GU212407, GU212408, GU212409, GU212410, GU212411, GU212412, GU212413, GU212414, GU212415, GU212416, GU212417, GU212418, GU212419, GU212420, GU212421, GU212422, GU212423, GU212424, GU212425, GU212426, GU212427, GU212428, GU212429, GU212430, GU212431, GU212432, GU212433, GU212434", "datasets": [{"dataset_uid": "000121", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "(Arenz et al. 2006) DQ317323, DQ317324, DQ317325, DQ317326, DQ317327, DQ317328, DQ317329, DQ317330, DQ317331, DQ317332, DQ317333, DQ317334, DQ317335, DQ317336, DQ317337, DQ317338, DQ317339, DQ317340, DQ317341, DQ317342, DQ317343, DQ317344, DQ317345, DQ317346, DQ317347, DQ317348, DQ317349, DQ317350, DQ317351, DQ317352, DQ317353, DQ317354, DQ317355, DQ317356, DQ317357, DQ317358, DQ317359, DQ317360, DQ317361, DQ317362, DQ317363, DQ317364, DQ317365, DQ317366, DQ317367, DQ317368, DQ317369, DQ317370, DQ317371, DQ317372, DQ317373, DQ317374, DQ317375, DQ317376, DQ317377, DQ317378, DQ317379, DQ317380, DQ317381, DQ317382, DQ317383, DQ317384, DQ317385, DQ317386, DQ317387, DQ317388, DQ317389 (Arenz and Blanchette 2009) FJ235934, FJ235935, FJ235936, FJ235937, FJ235938, FJ235939, FJ235940, FJ235941, FJ235942, FJ235943, FJ235944, FJ235945, FJ235946, FJ235947, FJ235948, FJ235949, FJ235950, FJ235951, FJ235952, FJ235953, FJ235954, FJ235955, FJ235956, FJ235957, FJ235958, FJ235959, FJ235960, FJ235961, FJ235962, FJ235963, FJ235964, FJ235965, FJ235966, FJ235967, FJ235968, FJ235969, FJ235970, FJ235971, FJ235972, FJ235973, FJ235974, FJ235975, FJ235976, FJ235977, FJ235978, FJ235979, FJ235980, FJ235981, FJ235982, FJ235983, FJ235984, FJ235985, FJ235986, FJ235987, FJ235988, FJ235989, FJ235990, FJ235991, FJ235992, FJ235993, FJ235994, FJ235995, FJ235996, FJ235997, FJ235998, FJ235999, FJ236000, FJ236001, FJ236002, FJ236003, FJ236004, FJ236005, FJ236006, FJ236007, FJ236008, FJ236009, FJ236010, FJ236011, FJ236012, FJ236013, FJ236014 (Blanchette et al. 2010) GU212367, GU212368, GU212369, GU212370, GU212371, GU212372, GU212373, GU212374, GU212375, GU212376, GU212377, GU212378, GU212379, GU212380, GU212381, GU212382, GU212383, GU212384, GU212385, GU212386, GU212387, GU212388, GU212389, GU212390, GU212391, GU212392, GU212393, GU212394, GU212395, GU212396, GU212397, GU212398, GU212399, GU212400, GU212401, GU212402, GU212403, GU212404, GU212405, GU212406, GU212407, GU212408, GU212409, GU212410, GU212411, GU212412, GU212413, GU212414, GU212415, GU212416, GU212417, GU212418, GU212419, GU212420, GU212421, GU212422, GU212423, GU212424, GU212425, GU212426, GU212427, GU212428, GU212429, GU212430, GU212431, GU212432, GU212433, GU212434", "url": "http://www.ncbi.nlm.nih.gov/genbank/"}], "date_created": "Mon, 24 May 2010 00:00:00 GMT", "description": "Fungi in Antarctic ecosystems are major contributors to biodiversity and have great influence on many processes such as biodegradation and nutrient cycling. It is essential for biological surveys as well as genomic and proteomic studies to be completed so a better understanding of these organisms is obtained. Previous research has identified unique fungi associated with historic wooden structures brought to Antarctica by Robert F. Scott and Ernest Shackleton during the Heroic Era of exploration. Many of the fungi found are previously undescribed species that belong to the little known genus Cadophora. The research team will obtain important new information on the fungi present in the Ross Sea and Peninsula Regions of Antarctica, particularly their role in decomposition and nutrient recycling and their mechanisms and strategies for survival in the polar environment. New tools and methods include denaturing gradient gel electrophoresis (DGGE), real-time PCR, and proteomic profiling. These analyses will reveal key details of the physiological adaptations these fungi have evolved to carry out processes such as biodegradation and nutrient cycling under conditions that would inhibit other fungi. This work, coupled with the training and learning opportunities it provides, will be of value to many fields of study including microbial ecology, polar biology, wood microbiology, environmental science, soil science, geobiochemistry, and mycology as well as fungal phylogenetics, proteomics and genomics. Results obtained will have immediate applied use to help preserve and protect Antarctica\u0027s historic monuments. The investigations proposed are a continuation of research to identify the microbes attacking these historic structures and artifacts and to elucidate their biology and ecology in the polar environment. New research will also be done at the historic Cape Adare huts, the first wooden structures to be built in Antarctica and also at East Base, an American historic site on Stonington Island from the Admiral Byrd and Ronne Expeditions of 1939-1948. The research team will conduct vital studies needed to successfully conserve the wooden structures and artifacts at these sites and protect them for future generations", "east": -62.0, "geometry": "POINT(-65.5 -65)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Blanchette, Robert", "platforms": "Not provided", "repo": "NCBI GenBank", "repositories": "NCBI GenBank", "science_programs": null, "south": -70.0, "title": "Studies of Antarctic Fungi: Adaptive Stratigies for Survival and Protecting Antarctica\u0027s Historic Structures", "uid": "p0000187", "west": -69.0}, {"awards": "0636773 DeMaster, David; 0636806 Smith, Craig", "bounds_geometry": "POLYGON((-71.2358 -52.7603,-69.75336 -52.7603,-68.27092 -52.7603,-66.78848 -52.7603,-65.30604 -52.7603,-63.8236 -52.7603,-62.34116 -52.7603,-60.85872 -52.7603,-59.37628 -52.7603,-57.89384 -52.7603,-56.4114 -52.7603,-56.4114 -54.29969,-56.4114 -55.83908,-56.4114 -57.37847,-56.4114 -58.91786,-56.4114 -60.45725,-56.4114 -61.99664,-56.4114 -63.53603,-56.4114 -65.07542,-56.4114 -66.61481,-56.4114 -68.1542,-57.89384 -68.1542,-59.37628 -68.1542,-60.85872 -68.1542,-62.34116 -68.1542,-63.8236 -68.1542,-65.30604 -68.1542,-66.78848 -68.1542,-68.27092 -68.1542,-69.75336 -68.1542,-71.2358 -68.1542,-71.2358 -66.61481,-71.2358 -65.07542,-71.2358 -63.53603,-71.2358 -61.99664,-71.2358 -60.45725,-71.2358 -58.91786,-71.2358 -57.37847,-71.2358 -55.83908,-71.2358 -54.29969,-71.2358 -52.7603))", "dataset_titles": "Expedition Data; Expedition data of LMG0802; Expedition data of LMG0902; Expedition Data of LMG0902; Expedition data of NBP0808; Labile Organic Carbon distributions on the West Antarctic Peninsula Shelf; Species List, Species Abundance, and Sediment Geochemistry processed data acquired during Laurence M. Gould expedition LMG0802", "datasets": [{"dataset_uid": "002611", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of NBP0808", "url": "https://www.rvdata.us/search/cruise/NBP0808"}, {"dataset_uid": "002725", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0802", "url": "https://www.rvdata.us/search/cruise/LMG0802"}, {"dataset_uid": "601319", "doi": "10.15784/601319", "keywords": "Antarctica; Antarctic Peninsula; Biota; Bioturbation Coefficients; Diagenesis; Labile Organic Carbon; LOC Mean Residence Times; Marguerite Bay; Oceans; Organic Carbon Degradation Rates; Sediment Core", "people": "Taylor, Richard; DeMaster, David; Thomas, Carrie; Smith, Craig; Isla, Enrique", "repository": "USAP-DC", "science_program": null, "title": "Labile Organic Carbon distributions on the West Antarctic Peninsula Shelf", "url": "https://www.usap-dc.org/view/dataset/601319"}, {"dataset_uid": "601303", "doi": null, "keywords": "Antarctica; Antarctic Peninsula; Biota; Box Corer; Chlorophyll Concentration; LMG0802; Marcofauna; Megafauna; Oceans; R/v Laurence M. Gould; Seafloor Sampling; Species Abundance", "people": "DeMaster, David; Smith, Craig", "repository": "USAP-DC", "science_program": null, "title": "Species List, Species Abundance, and Sediment Geochemistry processed data acquired during Laurence M. Gould expedition LMG0802", "url": "https://www.usap-dc.org/view/dataset/601303"}, {"dataset_uid": "001486", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data of LMG0902", "url": "https://www.rvdata.us/search/cruise/LMG0902"}, {"dataset_uid": "001513", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0802"}, {"dataset_uid": "002669", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0902", "url": "https://www.rvdata.us/search/cruise/LMG0902"}, {"dataset_uid": "002727", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0902", "url": "https://www.rvdata.us/search/cruise/LMG0902"}, {"dataset_uid": "002726", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0802", "url": "https://www.rvdata.us/search/cruise/LMG0802"}], "date_created": "Tue, 04 May 2010 00:00:00 GMT", "description": "The Antarctic Peninsula region exhibits one of the largest warming trends in the world. Climate change in this region will reduce the duration of winter sea-ice cover, altering both the pelagic ecosystem and bentho-pelagic coupling. We postulate that shelf benthic ecosystems are highly suitable for tracking climate change because they act as \"low-pass\" filters, removing high-frequency seasonal noise and responding to longer-term trends in pelagic ecosystem structure and export production. We propose to conduct a 3-year study of bentho-pelagic coupling along a latitudinal climate gradient on the Antarctic Peninsula to explore the potential impacts of climate change (e.g., reduction in sea-ice duration) on Antarctic shelf ecosystems. We will conduct three cruises during summer and winter regimes along a 5- station transect from Smith Island to Marguerite Bay, evaluating a broad range of benthic ecological and biogeochemical processes. Specifically, we will examine the feeding strategies of benthic deposit feeders along this climatic gradient to elucidate the potential response of this major trophic group to climatic warming. In addition, we will (1) quantify carbon and nitrogen cycling and burial at the seafloor and (2) document changes in megafaunal, macrofaunal, and microbial community structure along this latitudinal gradient. We expect to develop predictive insights into the response of Antarctic shelf ecosystems to some of the effects of climate warming (e.g., a reduction in winter sea-ice duration). The proposed research will considerably broaden the ecological and carbon-flux measurements made as parts of the Palmer Station LTER and GLOBEC programs by providing a complementary benthic component. This project also will promote science education from the 9th grade to graduate-student levels. We will partner with the NSF-sponsored Southeastern Center for Ocean Science Education Excellence to reach students of all races in all areas of NC, SC and GA. The project will also benefit students at the post secondary level by supporting three graduate and two undergraduate students. During each of the three field excursions, NCSU and UH students will travel to Chile and Antarctica to participate in scientific research. Lastly, all three PIs will incorporate material from this project into their undergraduate and graduate courses.", "east": -56.4114, "geometry": "POINT(-63.8236 -60.45725)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; IN SITU/LABORATORY INSTRUMENTS \u003e PRESSURE/HEIGHT METERS \u003e PRESSURE SENSORS; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ADCP; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; IN SITU/LABORATORY INSTRUMENTS \u003e MAGNETIC/MOTION SENSORS \u003e GRAVIMETERS \u003e GRAVIMETERS; IN SITU/LABORATORY INSTRUMENTS \u003e PRESSURE/HEIGHT METERS \u003e PRESSURE SENSORS; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ADCP; IN SITU/LABORATORY INSTRUMENTS \u003e PHOTON/OPTICAL DETECTORS \u003e TURBIDITY METERS", "is_usap_dc": false, "keywords": "LMG0802; R/V LMG; AMD; Amd/Us; LMG0902; USA/NSF; NBP0808; USAP-DC; R/V NBP", "locations": null, "north": -52.7603, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "DeMaster, David; Smith, Craig", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG; WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repo": "R2R", "repositories": "R2R; USAP-DC", "science_programs": null, "south": -68.1542, "title": "Collaborative Research: Benthic Faunal Feeding Dynamics on the Antarctic Shelf and the Effects of Global Climate Change on Bentho-Pelagic Coupling", "uid": "p0000552", "west": -71.2358}, {"awards": "9714299 Caron, David", "bounds_geometry": "POLYGON((-179.9984 -43.56676,-143.99865 -43.56676,-107.9989 -43.56676,-71.99915 -43.56676,-35.9994 -43.56676,0.000349999999997 -43.56676,36.0001 -43.56676,71.99985 -43.56676,107.9996 -43.56676,143.99935 -43.56676,179.9991 -43.56676,179.9991 -47.057693,179.9991 -50.548626,179.9991 -54.039559,179.9991 -57.530492,179.9991 -61.021425,179.9991 -64.512358,179.9991 -68.003291,179.9991 -71.494224,179.9991 -74.985157,179.9991 -78.47609,143.99935 -78.47609,107.9996 -78.47609,71.99985 -78.47609,36.0001 -78.47609,0.000349999999997 -78.47609,-35.9994 -78.47609,-71.99915 -78.47609,-107.9989 -78.47609,-143.99865 -78.47609,-179.9984 -78.47609,-179.9984 -74.985157,-179.9984 -71.494224,-179.9984 -68.003291,-179.9984 -64.512358,-179.9984 -61.021425,-179.9984 -57.530492,-179.9984 -54.039559,-179.9984 -50.548626,-179.9984 -47.057693,-179.9984 -43.56676))", "dataset_titles": "Expedition Data", "datasets": [{"dataset_uid": "002003", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/NBP9901"}], "date_created": "Tue, 04 May 2010 00:00:00 GMT", "description": "*** Caron 9714299 The analysis of microbial biodiversity of extreme environments is difficult because traditional methods for examining diversity are often ineffective for assessing species richness within these communities. Additional difficulties arise due to the difficulties of recreating and maintaining pertinent environmental features during sample collection and procession. This study focuses on the protistan assemblages (algae and protozoa) in the sea ice, sediment and ocean environments of the Ross Sea, Antarctica. The identification of protistan species in natural assemblages traditionally has entailed direct microscopical analyses as well as enrichment and culture techniques for assessing biodiversity. Determination of diversity for these assemblages in therefore susceptible to biases as a consequence of sampling, enrichment and culture, as well as selective losses due to sample preservation and concentration for microscopy. The goals of this project are: (1) to develop and apply molecular biological approaches to assess species diversity of small protists (algae and protozoa smaller than 100 micrometers) in ocean water, sea ice and sediment environments and (2) to obtain baseline physiological information on the growth rates, feeding rates and growth efficiencies of cultured protozoa under pertinent temperature regimes. Molecular biological studies will involve the use of PCR-based protocols to examine small subunit ribosomal RNA gene (srDNA) diversity. Approaches and techniques developed will be applicable to any other water body or sediment and would provide a means to examine the representativeness of protistan cultures in extant culture collections. ***", "east": 179.9991, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": false, "keywords": "R/V NBP", "locations": null, "north": -43.56676, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Caron, David; Jeffries, Martin", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repo": "R2R", "repositories": "R2R", "science_programs": null, "south": -78.47609, "title": "LEXEN: Protistan Biodiversity in Antarctic Marine Ecosystems: Molecular Biological and Traditional Approaches", "uid": "p0000625", "west": -179.9984}, {"awards": "9910098 Fritsen, Christian", "bounds_geometry": null, "dataset_titles": "Expedition Data; Expedition data of LMG0106; Expedition data of LMG0205; Expedition data of NBP0104; Expedition data of NBP0204", "datasets": [{"dataset_uid": "002643", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of NBP0204", "url": "https://www.rvdata.us/search/cruise/NBP0204"}, {"dataset_uid": "002657", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of NBP0104", "url": "https://www.rvdata.us/search/cruise/NBP0104"}, {"dataset_uid": "002695", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0106", "url": "https://www.rvdata.us/search/cruise/LMG0106"}, {"dataset_uid": "001757", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0205"}, {"dataset_uid": "002702", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0205", "url": "https://www.rvdata.us/search/cruise/LMG0205"}], "date_created": "Tue, 04 May 2010 00:00:00 GMT", "description": "The U.S. Global Ocean Ecosystems Dynamics (U.S. GLOBEC) program has the goal of understanding and ultimately predicting how populations of marine animal species respond to natural and anthropogenic changes in climate. Research in the Southern Ocean (SO) indicates strong coupling between climatic processes and ecosystem dynamics via the annual formation and destruction of sea ice. The Southern Ocean GLOBEC Program (SO GLOBEC) will investigate the dynamic relationship between physical processes and ecosystem responses through identification of critical parameters that affect the distribution, abundance and population dynamics of target species. The overall goals of the SO GLOBEC program are to elucidate shelf circulation processes and their effect on sea ice formation and krill distribution, and to examine the factors which govern krill survivorship and availability to higher trophic levels, including penguins, seals and whales. The focus of the U.S. contribution to the international SO GLOBEC program will be on winter processes. This component will focus on the distribution and activities of sea ice microbial communities. This will be accomplished using an integrated combination of sampling (vertical profiles, horizontal surveys, and under-ice surveys) and observational protocols. Experiments will be designed to estimate microbial activity within the sea ice and at the ice-seawater interface. The research will be coordinated with components studying the water column productivity and the sea ice habitat. The result of the integrated SO GLOBEC program will be to improve the predictability of living marine resources, especially with respect to local and global climatic shifts.", "east": null, "geometry": null, "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e MAGNETIC/MOTION SENSORS \u003e GRAVIMETERS \u003e GRAVIMETERS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e MSBS", "is_usap_dc": false, "keywords": "R/V LMG; R/V NBP", "locations": null, "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Fritsen, Christian; Costa, Daniel", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG; WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repo": "R2R", "repositories": "R2R", "science_programs": null, "south": null, "title": "GLOBEC: Sea Ice Microbial Communities", "uid": "p0000834", "west": null}, {"awards": "0632250 Cary, Stephen", "bounds_geometry": "POLYGON((-38.5 -72.6,-23.963 -72.6,-9.426 -72.6,5.111 -72.6,19.648 -72.6,34.185 -72.6,48.722 -72.6,63.259 -72.6,77.796 -72.6,92.333 -72.6,106.87 -72.6,106.87 -73.185,106.87 -73.77,106.87 -74.355,106.87 -74.94,106.87 -75.525,106.87 -76.11,106.87 -76.695,106.87 -77.28,106.87 -77.865,106.87 -78.45,92.333 -78.45,77.796 -78.45,63.259 -78.45,48.722 -78.45,34.185 -78.45,19.648 -78.45,5.111 -78.45,-9.426 -78.45,-23.963 -78.45,-38.5 -78.45,-38.5 -77.865,-38.5 -77.28,-38.5 -76.695,-38.5 -76.11,-38.5 -75.525,-38.5 -74.94,-38.5 -74.355,-38.5 -73.77,-38.5 -73.185,-38.5 -72.6))", "dataset_titles": "Metagenomic Data Lake Vostok Microbial Community", "datasets": [{"dataset_uid": "000136", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Metagenomic Data Lake Vostok Microbial Community", "url": "http://www.ncbi.nlm.nih.gov/genbank/"}], "date_created": "Fri, 30 Apr 2010 00:00:00 GMT", "description": "This project brings together researchers with expertise in molecular microbial ecology, Antarctic and deep sea environments, and metagenomics to address the overarching question: how do ecosystems dominated by microorganisms adapt to conditions of continuous cold and dark over evolutionarily and geologically relevant time scales? Lake Vostok, buried for at least 15 million years beneath approximately 4 km of ice that has prevented any communication with the external environment for as much as 1.5 million years, is an ideal system to study this question. Water from the lake that has frozen on to the bottom of the ice sheet (accretion ice) is available for study. Several studies have indicated the presence of low abundance, but detectable microbial communities in the accretion ice. Our central hypothesis maintains that Lake Vostok microbes are specifically adapted to life in conditions of extreme cold, dark, and oligotrophy and that signatures of those adaptations can be observed in their genome sequences at the gene, organism, and community levels. To address this hypothesis, we propose to characterize the metagenome (i.e. the genomes of all members of the community) of the accretion ice. using whole genome amplification (WGA), which can provide micrograms of unbiased metagenomic DNA from only a few cells. The results of this project have relevance to evolutionary biology and ecology, subglacial Antarctic lake exploration, biotechnology, and astrobiology. The project directly addresses priorities and themes in the International Polar Year at the national and international levels. A legacy of DNA sequence data and the metagenomic library will be created and maintained. Press releases and a publicly available web page will facilitate communication with the public. K-12 outreach will be the focus of a new, two-tiered program targeting the 7th grade classroom and on site visits to the Joint Genome Institute Production Sequencing Facility by high school juniors and seniors and community college level students. Minority undergraduate researchers will be recruited for research on this project, and support and training are provided to two graduate students, a postdoctoral scholar, and a technician.", "east": 106.87, "geometry": "POINT(34.185 -75.525)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -72.6, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Cary, Stephen", "platforms": "Not provided", "repo": "NCBI GenBank", "repositories": "NCBI GenBank", "science_programs": null, "south": -78.45, "title": "IPY: Collaborative Research: A Metagenomic Investigation of Adaptation to Prolonged Cold and Dark Conditions of the Lake Vostok Microbial Community", "uid": "p0000201", "west": -38.5}, {"awards": "0739712 Staudigel, Hubertus", "bounds_geometry": "POLYGON((167.04 -77.51,167.067 -77.51,167.094 -77.51,167.121 -77.51,167.148 -77.51,167.175 -77.51,167.202 -77.51,167.229 -77.51,167.256 -77.51,167.283 -77.51,167.31 -77.51,167.31 -77.513,167.31 -77.516,167.31 -77.519,167.31 -77.522,167.31 -77.525,167.31 -77.528,167.31 -77.531,167.31 -77.534,167.31 -77.537,167.31 -77.54,167.283 -77.54,167.256 -77.54,167.229 -77.54,167.202 -77.54,167.175 -77.54,167.148 -77.54,167.121 -77.54,167.094 -77.54,167.067 -77.54,167.04 -77.54,167.04 -77.537,167.04 -77.534,167.04 -77.531,167.04 -77.528,167.04 -77.525,167.04 -77.522,167.04 -77.519,167.04 -77.516,167.04 -77.513,167.04 -77.51))", "dataset_titles": "Metagenome from fumarole sediments sampled from Warren Cave, Antarctica", "datasets": [{"dataset_uid": "000213", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Metagenome from fumarole sediments sampled from Warren Cave, Antarctica", "url": "http://www.ncbi.nlm.nih.gov/bioproject/PRJNA255918"}], "date_created": "Mon, 22 Jun 2009 00:00:00 GMT", "description": "This project studies the microbial processes that alter volcanic glass, which is critical to understanding the earliest life on earth. To understand the environmental controls on these processes, this project uses the extreme environments of the McMurdo region of Antarctica as a natural laboratory. Volcanic glass substrates are placed in hydrothermal systems, lakes, and other areas for two to four years to identify colonizing microbial consortia and the chemical processes of microbe-glass interaction. Recovered experiments are analyzed to explore the role of eukaryotic and prokaryotic organisms, and the relevance of autotrophs during colonization and biofilm formation using microscopic, molecular and culture techniques. \u003cbr/\u003e\u003cbr/\u003eThe broader impacts include graduate and undergraduate student participation in research and K-12 outreach and teacher training.", "east": 167.31, "geometry": "POINT(167.175 -77.525)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -77.51, "nsf_funding_programs": "Antarctic Earth Sciences", "paleo_time": null, "persons": "Staudigel, Hubertus", "platforms": "Not provided", "repo": "NCBI GenBank", "repositories": "NCBI GenBank", "science_programs": null, "south": -77.54, "title": "Collaborative Research: Microbially Mediated Alteration of Volcanic Glass using McMurdo Extreme Environments as Natural Laboratories", "uid": "p0000545", "west": 167.04}, {"awards": "0338260 Chin, Yu-Ping; 0338342 Foreman, Christine", "bounds_geometry": "POINT(166.167 -77.55)", "dataset_titles": "Biogeochemistry of Dissolved Organic Matter in Pony Lake, Ross Island", "datasets": [{"dataset_uid": "600168", "doi": "10.15784/600168", "keywords": "Antarctica; Chemistry:fluid; Chemistry:Fluid; Critical Zone; Ross Island; Sample/collection Description; Sample/Collection Description; Water Samples", "people": "Foreman, Christine; Chin, Yu-Ping", "repository": "USAP-DC", "science_program": null, "title": "Biogeochemistry of Dissolved Organic Matter in Pony Lake, Ross Island", "url": "https://www.usap-dc.org/view/dataset/600168"}], "date_created": "Mon, 16 Mar 2009 00:00:00 GMT", "description": "Dissolved organic matter (DOM) is a significant chemical component in aquatic systems because it acts as an important carbon source for microorganisms, absorbs harmful radiation in sunlight, is able to complex metals, and can participate in important biogeochemical reactions. This study will investigate the biogeochemical cycling of DOM in a small coastal Antarctic pond, Pony Lake, located on Cape Royds, Ross Island. Because there are no higher plants present at this site all of the DOM in this lake is derived from microorganisms. Thus, Pony Lake is an ideal site to study the effect of physical, chemical, and microbial processes on the composition and character of the DOM pool. Finally, Pony Lake is also an ideal site to collect an International Humic Substances Society (IHSS) fulvic acid standard. Unlike other IHSS standards, this standard will not contain DOM components derived from higher land plants. To better understand the role of physical influences, the project will study the changes in the DOM pool as the lake evolves from ice-covered to ice-free conditions during the summer, as well as the relationship of DOM to the observed turnover of dominant microbial communities in the lake. Scientists will also monitor changes in microbial abundance, diversity, and productivity that may occur during the ice to open-water transition period. This research will provide much needed information regarding the relationship between microbial diversity and DOM biogeochemistry. Middle school science students will be active participants in this project through the Internet, while scientists are in the field, and in the lab.", "east": 166.167, "geometry": "POINT(166.167 -77.55)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided; FIELD SURVEYS", "locations": null, "north": -77.55, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Foreman, Christine; Chin, Yu-Ping", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS; Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -77.55, "title": "Collaborative Research: Biogeochemistry of Dissolved Organic Matter in Pony Lake, Ross Island", "uid": "p0000548", "west": 166.167}, {"awards": "0742057 Gallager, Scott", "bounds_geometry": "POLYGON((-168.291 -64.846,-165.018 -64.846,-161.745 -64.846,-158.472 -64.846,-155.199 -64.846,-151.926 -64.846,-148.653 -64.846,-145.38 -64.846,-142.107 -64.846,-138.834 -64.846,-135.561 -64.846,-135.561 -66.0269,-135.561 -67.2078,-135.561 -68.3887,-135.561 -69.5696,-135.561 -70.7505,-135.561 -71.9314,-135.561 -73.1123,-135.561 -74.2932,-135.561 -75.4741,-135.561 -76.655,-138.834 -76.655,-142.107 -76.655,-145.38 -76.655,-148.653 -76.655,-151.926 -76.655,-155.199 -76.655,-158.472 -76.655,-161.745 -76.655,-165.018 -76.655,-168.291 -76.655,-168.291 -75.4741,-168.291 -74.2932,-168.291 -73.1123,-168.291 -71.9314,-168.291 -70.7505,-168.291 -69.5696,-168.291 -68.3887,-168.291 -67.2078,-168.291 -66.0269,-168.291 -64.846))", "dataset_titles": "SGER: Primary and Secondary Production and Carbon Flux Through the Microbial Community Along the Western Antarctic Marginal Ice Zone on the Oden Southern Ocean 2007 Expeditions", "datasets": [{"dataset_uid": "600086", "doi": "10.15784/600086", "keywords": "Amundsen Sea; Biota; Microbiology; Navigation; Oceans; Oden; OSO2007; Sample/collection Description; Sample/Collection Description; Southern Ocean", "people": "Dennett, Mark; Gallager, Scott", "repository": "USAP-DC", "science_program": null, "title": "SGER: Primary and Secondary Production and Carbon Flux Through the Microbial Community Along the Western Antarctic Marginal Ice Zone on the Oden Southern Ocean 2007 Expeditions", "url": "https://www.usap-dc.org/view/dataset/600086"}], "date_created": "Mon, 16 Mar 2009 00:00:00 GMT", "description": "Abstract\u003cbr/\u003e\u003cbr/\u003eThe research will continue and extend the study in the Southern Ocean that was initiated during the Oden Southern Ocean 2006 expedition in collaboration with Swedish scientist Mellissa Chierici. We will quantify carbon flux through the food web in the marginal ice zone (MIZ) by measuring size fractionated primary and secondary production, grazing and carbon flux through nanoplankton (2-20 um), microplankton (20-200um), and mesoplankton (200-2000 um). Community structure, species abundance and size specific grazing rates will be quantified using a variety of techniques both underway and at ice stations along the MIZ. The proposed cruise track extends across the Drake Passage to the Western Antarctic Peninsula (WAP) with three station transects along a gradient from the open ocean through the marginal ice zone (MIZ) in the Bellinghausen and Amundsen Seas and into the Ross Sea Polynya. Ice stations along each transect will provide material to characterize production associated with annual ice. Underway measurements of primary and secondary production (chlorophyll, CDOM, microplankton, and mesoplankton) and hydrography (temperature, salinity, pH, DO, turbidity) will establish a baseline for future cruises and as support for other projects such as biogeochemical studies on carbon dioxide drawdown and trace metal work on primary production. The outcome of these measurements will be a description of nano to mesoplankton standing stocks, community structure, and carbon flux along the MIZ in the Bellinghausen and Amundsen Seas and the Ross Sea Polynya.", "east": -135.561, "geometry": "POINT(-151.926 -70.7505)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -64.846, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Gallager, Scott; Dennett, Mark", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -76.655, "title": "SGER: Primary and Secondary Production and Carbon Flux Through the Microbial Community Along the Western Antarctic Marginal Ice Zone on the Oden Southern Ocean 2007 Expeditions", "uid": "p0000563", "west": -168.291}, {"awards": "0230276 Ward, Bess", "bounds_geometry": "POLYGON((162 -77.2,162.16 -77.2,162.32 -77.2,162.48 -77.2,162.64 -77.2,162.8 -77.2,162.96 -77.2,163.12 -77.2,163.28 -77.2,163.44 -77.2,163.6 -77.2,163.6 -77.26,163.6 -77.32,163.6 -77.38,163.6 -77.44,163.6 -77.5,163.6 -77.56,163.6 -77.62,163.6 -77.68,163.6 -77.74,163.6 -77.8,163.44 -77.8,163.28 -77.8,163.12 -77.8,162.96 -77.8,162.8 -77.8,162.64 -77.8,162.48 -77.8,162.32 -77.8,162.16 -77.8,162 -77.8,162 -77.74,162 -77.68,162 -77.62,162 -77.56,162 -77.5,162 -77.44,162 -77.38,162 -77.32,162 -77.26,162 -77.2))", "dataset_titles": "What Limits Denitrification and Bacterial Growth in Lake Bonney, Taylor Valley, Antarctica?", "datasets": [{"dataset_uid": "600033", "doi": "10.15784/600033", "keywords": "Antarctica; Biota; CTD Data; Dry Valleys; Lake Bonney; Lake Vanda; Microbiology; Taylor Valley", "people": "Ward, Bess", "repository": "USAP-DC", "science_program": null, "title": "What Limits Denitrification and Bacterial Growth in Lake Bonney, Taylor Valley, Antarctica?", "url": "https://www.usap-dc.org/view/dataset/600033"}], "date_created": "Sun, 18 Jan 2009 00:00:00 GMT", "description": "Denitrification is the main process by which fixed nitrogen is lost from ecosystems and the regulation of this process may directly affect primary production and carbon cycling over short and long time scales. Previous investigations of the role of bioactive metals in regulating denitrification in bacteria from permanently ice-covered Lake Bonney in the Taylor Valley of East Antarctica indicated that denitrifying bacteria can be negatively affected by metals such as copper, iron, cadmium, lead, chromium, nickel, silver and zinc; and that there is a distinct difference in denitrifying activity between the east and west lobes of the lake. Low iron concentrations were found to exacerbate the potential toxicity of the other metals, while silver has the potential to specifically inhibit denitrification because of its ability to interfere with copper binding in redox proteins, such as nitrite reductase and nitrous oxide reductase. High silver concentrations might prevent the functioning of nitrous oxide reductase in the same way that simple copper limitation does, thereby causing the buildup of nitrous oxide and resulting in a nonfunctional nitrogen cycle. Other factors, such as oxygen concentration, are likely also to affect bacterial activity in Lake Bonney. This project will investigate silver toxicity, general metal toxicity and oxygen concentration to determine their effect on denitrification in the lake by using a suite of \"sentinel\" strains of denitrifying bacteria (isolated from the lake) incubated in Lake Bonney water and subjected to various treatments. The physiological responses of these strains to changes in metal and oxygen concentration will be quantified by flow cytometric detection of single cell molecular probes whose sensitivity and interpretation have been optimized for the sentinel strains. Understanding the relationships between metals and denitrification is expected to enhance our understanding of not only Lake Bonney\u0027s unusual nitrogen cycle, but more generally, of the potential role of metals in the regulation of microbial nitrogen transformations.\u003cbr/\u003e\u003cbr/\u003eThe broader impacts of this work include not only a better understanding of regional biogeochemistry and global perspectives on these processes; but also the training of graduate students and a substantial outreach effort for school children.", "east": 163.6, "geometry": "POINT(162.8 -77.5)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD", "is_usap_dc": true, "keywords": "FIELD SURVEYS", "locations": null, "north": -77.2, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Ward, Bess", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -77.8, "title": "Collaborative Research: What Limits Denitrification and Bacterial Growth in Lake Bonney, Taylor Valley, Antarctica?", "uid": "p0000223", "west": 162.0}, {"awards": "0338267 Gooseff, Michael", "bounds_geometry": "POLYGON((161.6 -77.4,161.773 -77.4,161.946 -77.4,162.119 -77.4,162.292 -77.4,162.465 -77.4,162.638 -77.4,162.811 -77.4,162.984 -77.4,163.157 -77.4,163.33 -77.4,163.33 -77.435,163.33 -77.47,163.33 -77.505,163.33 -77.54,163.33 -77.575,163.33 -77.61,163.33 -77.645,163.33 -77.68,163.33 -77.715,163.33 -77.75,163.157 -77.75,162.984 -77.75,162.811 -77.75,162.638 -77.75,162.465 -77.75,162.292 -77.75,162.119 -77.75,161.946 -77.75,161.773 -77.75,161.6 -77.75,161.6 -77.715,161.6 -77.68,161.6 -77.645,161.6 -77.61,161.6 -77.575,161.6 -77.54,161.6 -77.505,161.6 -77.47,161.6 -77.435,161.6 -77.4))", "dataset_titles": "Antarctic Hydrologic Margin Microbiology and Biogeochemistry - data; Hydrologic Margins Research Project, 2004-2008, McMurdo Dry Valleys", "datasets": [{"dataset_uid": "000238", "doi": "", "keywords": null, "people": null, "repository": "PI website", "science_program": null, "title": "Antarctic Hydrologic Margin Microbiology and Biogeochemistry - data", "url": "http://water.engr.psu.edu/gooseff/web_antarctica/data.html"}, {"dataset_uid": "600016", "doi": "", "keywords": null, "people": "Gooseff, Michael N.", "repository": "USAP-DC", "science_program": null, "title": "Hydrologic Margins Research Project, 2004-2008, McMurdo Dry Valleys", "url": "https://www.usap-dc.org/view/dataset/600016"}], "date_created": "Thu, 11 Sep 2008 00:00:00 GMT", "description": "Aquatic-terrestrial transition zones are crucial environments in understanding the biogeochemistry of landscapes. In temperate watersheds, these areas are generally dominated by riparian zones, which have been identified as regions of special interest for biogeochemistry because of the increased microbial activity in these locations, and because of the importance of these hydrological margins in facilitating and buffering hydrologic and biogeochemical exchanges between terrestrial and aquatic ecosystems. In the Antarctic Dry Valleys, terrestrial-aquatic transition zones are intriguing landscape features because of the vast importance of water in this polar desert, and because the material and energy budgets of dry valley ecosystems are linked by hydrology. Hydrological margins in aquatic-terrestrial transition zones will be studied in the Dry Valleys of Antarctica to answer two overarching questions: (1) what are the major controls over hydrologic and biogeochemical exchange across aquaticterrestrial transition zones and (2) to what extent do trends in nutrient cycling (e.g. nitrogen cycling) across these transition zones reflect differences in microbial communities or function vs. differences in the physical and chemical environment (e.g., redox potential)? The hydrologic gradients that define these interfaces provide the opportunity to assess the relative influence of physical conditions and microbial biodiversity and functioning upon biogeochemical cycling. Coordinated hydrologic, biogeochemical, and molecular microbial studies will be executed within hydrologic margins with the following research objectives: to determine the role of sediment characteristics, permafrost and active layer dynamics, and topography on sub-surface water content and distribution in hydrologic margins, to determine the extent to which transformations of nitrogen in hydrological margins are influenced by physical conditions (i.e., moisture, redox potential and pH) or by the presence of specific microbial communities (e.g., denitrifiers), and to characterize the microbial community structure and function of saturated zones.\u003cbr/\u003e\u003cbr/\u003eThis proposed research will provide an improved understanding of the interaction of liquid water, soils, microbial communities, and biogeochemistry within the important hydrologic margin landscape units of the dry valleys. Dry valleys streams and lakes are unique because there is no influence of higher vegetation on the movement of water and may therefore provide a model system for understanding physical and hydrological influences on microbial ecology and biogeochemistry. Hence the findings will contribute to Antarctic science as well as the broader study of riparian zones and hydrologic margins worldwide. Graduate students and undergraduate students will be involved with fieldwork and research projects. Information will be disseminated through a project web site, and outreach activities will include science education in local elementary, middle and high schools near the three universities involved.", "east": 163.33, "geometry": "POINT(162.465 -77.575)", "instruments": null, "is_usap_dc": false, "keywords": "Not provided", "locations": null, "north": -77.4, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Gooseff, Michael N.; Barrett, John; Takacs-Vesbach, Cristina", "platforms": "Not provided", "repo": "PI website", "repositories": "PI website; USAP-DC", "science_programs": null, "south": -77.75, "title": "Collaborative Research: Hydrologic Controls over Biogeochemistry and Microbial Community Structure and Function across Terrestrial/Aquatic Interfaces in a Polar Desert", "uid": "p0000340", "west": 161.6}, {"awards": "0536870 Rogers, Scott", "bounds_geometry": "POINT(-106.8 -72.4667)", "dataset_titles": "Comprehensive Biological Study of Vostok Accretion Ice", "datasets": [{"dataset_uid": "600052", "doi": "10.15784/600052", "keywords": "Antarctica; Biota; Cryosphere; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Hydrothermal Vent; Lake Vostok; Microbes; Subglacial Lake", "people": "Rogers, Scott O.", "repository": "USAP-DC", "science_program": null, "title": "Comprehensive Biological Study of Vostok Accretion Ice", "url": "https://www.usap-dc.org/view/dataset/600052"}], "date_created": "Tue, 02 Sep 2008 00:00:00 GMT", "description": "The large subglacial Lake Vostok in Antarctica is unique ecological site with a novel microbial biota. The temperatures, pressures and lack of light all select for organisms that may not exist anywhere else on Earth. The accretion ice (lake water frozen to the bottom of the lower surface of the glacier) has preserved microbial samples from each region of Lake Vostok as the glacier passes over and into the lake. Thus, without contaminating the lake with microorganisms from the surface, microbes originating from the lake can be collected, transported to the laboratory and studied. Two of the deepest ice cores sections in this project are part of the international allocation. The will be shared between four researchers (Sergey Bulat from Russia, Jean-Robert Petit and Daniel Prieur from France, Scott Rogers from USA). The United States team will study, isolate, and characterize bacteria, fungi, and viruses that have been sampled from the lake through the process of ice accretion to the lower surface of 3500+m thick glacier overriding the lake. The project will involve a suite of methods, including molecular, morphological, and cultural. This includes observation and description by fluorescence, light, and electron microscopy, isolation on thirteen separate cultural media, polymerase chain reaction amplification, DNA sequencing, and phylogenetic analyses. Eleven accretion ice core sections, as well as two glacial ice core sections. As well as two glacial ice core sections will be studied. The accretion ice core sections, as well as two glacial ice core sections will be studied. The accretion ice core sections represent all of the major regions of the lake that have been sampled by the accretion process in the vicinity of the Vostok 5G ice core. The broader impacts of the work relate to the impact the results will have on the filed. These long=isolated lakes, deep below the Antarctic ice sheet may contain novel uniquely adapted organisms. Glacial ice contains an enormous diversity of entrapped microbes, some of which may be metabolically active in the ice. The microbes from Lake Vostok are of special interest, since they are adapted to cold, dark, and high pressure. Thus, their enzyme systems and biochemical pathways may be significantly different from those in the microbes that are the subject of current studies. As such, these organisms may form compounds that may have useful applications. Also, study of the accretion ice, and eventually the water, from Lake Vostok will provide a basis for the study of other subglacial lakes. Additionally, study of the microbes in the accretion ice will be useful to those planning to study analogous systems on ice-covered planets and moons.", "east": -106.8, "geometry": "POINT(-106.8 -72.4667)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -72.4667, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Rogers, Scott O.", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -72.4667, "title": "Comprehensive Biological Study of Vostok Accretion Ice", "uid": "p0000566", "west": -106.8}, {"awards": "0127022 Jeffrey, Wade", "bounds_geometry": "POLYGON((-177.639 -43.5676,-143.1091 -43.5676,-108.5792 -43.5676,-74.0493 -43.5676,-39.5194 -43.5676,-4.9895 -43.5676,29.5404 -43.5676,64.0703 -43.5676,98.6002 -43.5676,133.1301 -43.5676,167.66 -43.5676,167.66 -46.99877,167.66 -50.42994,167.66 -53.86111,167.66 -57.29228,167.66 -60.72345,167.66 -64.15462,167.66 -67.58579,167.66 -71.01696,167.66 -74.44813,167.66 -77.8793,133.1301 -77.8793,98.6002 -77.8793,64.0703 -77.8793,29.5404 -77.8793,-4.9895 -77.8793,-39.5194 -77.8793,-74.0493 -77.8793,-108.5792 -77.8793,-143.1091 -77.8793,-177.639 -77.8793,-177.639 -74.44813,-177.639 -71.01696,-177.639 -67.58579,-177.639 -64.15462,-177.639 -60.72345,-177.639 -57.29228,-177.639 -53.86111,-177.639 -50.42994,-177.639 -46.99877,-177.639 -43.5676))", "dataset_titles": "Expedition Data; Ross Sea microbial biomass and production", "datasets": [{"dataset_uid": "600029", "doi": "10.15784/600029", "keywords": "Biota; Chemistry:fluid; Chemistry:Fluid; CTD Data; Microbiology; Oceans; Phytoplankton; Ross Sea; Southern Ocean", "people": "Jeffrey, Wade H.", "repository": "USAP-DC", "science_program": null, "title": "Ross Sea microbial biomass and production", "url": "https://www.usap-dc.org/view/dataset/600029"}, {"dataset_uid": "001584", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/NBP0508"}, {"dataset_uid": "001690", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/NBP0304B"}], "date_created": "Thu, 12 Jun 2008 00:00:00 GMT", "description": "Ultraviolet radiation influences the dynamics of plankton processes in the near-surface waters of most aquatic ecosystems. In particular, the Southern Ocean is affected in the austral spring period when biologically damaging ultraviolet radiation is enhanced by ozone depletion. While progress has been made in estimating the quantitative impact of ultraviolet radiation on bacteria and phytoplankton in the Southern Ocean, some important issues remain to be resolved. Little is known about responses in systems dominated by the colonial haptophyte Phaeocystis antarctica, which dominates spring blooms in a polyna that develops in the southern Ross Sea. The Ross Sea is also of interest because of the occurrence of open water at a far southerly location in the spring, well within the ozone hole, and continuous daylight, with implications for the regulation of DNA repair. A number of studies suggest that vertical mixing can significant modify the impact of ultraviolet radiation in the Southern Ocean and elsewhere. However, there are limited measurements of turbulence intensity in the surface layer and measurements have not been integrated with parallel studies of ultraviolet radiation effects on phytoplankton and bacterioplankton. To address these issues, this collaborative study will focus on vertical mixing and the impact of ultraviolet radiation in the Ross Sea. The spectral and temporal responses of phytoplankton and bacterioplankton to ultraviolet radiation will be characterized in both laboratory and solar incubations. These will lead to the definition of biological weighting functions and response models capable of predicting the depth and time distribution of ultraviolet radiation impacts on photosynthesis, bacterial incorporation and DNA damage in the surface layer. Diel sampling will measure depth-dependent profiles of DNA damage, bacterial incorporation, photosynthesis and fluorescence parameters over a 24 h cycle. Sampling will include stations with contrasting wind-driven mixing and stratification as the polyna develops. The program of vertical mixing measurements is optimized for the typical springtime Ross Sea situation in which turbulence of intermediate intensity is insufficient to mix the upper layer thoroughly in the presence of stabilizing influences like solar heating and/or surface freshwater input from melting ice. Fine-scale vertical density profiles will be measured with a free-fall CTD unit and the profiles will be used to directly estimate large-eddy scales by determining Thorpe scales. Eddy scales and estimated turbulent diffusivities will be directly related to surface layer effects, and used to generate lagrangian depth-time trajectories in models of ultraviolet radiation responses in the surface mixed layer. The proposed research will be the first in-depth study of ultraviolet radiation effects in the Ross Sea and provide a valuable comparison with previous work in the Weddell-Scotia Confluence and Palmer Station regions. It will also enhance the understanding of vertical mixing processes, trophic interactions and biogeochemical cycling in the Ross Sea.", "east": 167.66, "geometry": "POINT(-4.9895 -60.72345)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; IN SITU/LABORATORY INSTRUMENTS \u003e MAGNETIC/MOTION SENSORS \u003e GRAVIMETERS \u003e GRAVIMETERS; IN SITU/LABORATORY INSTRUMENTS \u003e PRESSURE/HEIGHT METERS \u003e PRESSURE SENSORS; IN SITU/LABORATORY INSTRUMENTS \u003e PHOTON/OPTICAL DETECTORS \u003e TURBIDITY METERS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ADCP; IN SITU/LABORATORY INSTRUMENTS \u003e SAMPLERS \u003e BOTTLES/FLASKS/JARS \u003e WATER BOTTLES; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e MSBS; IN SITU/LABORATORY INSTRUMENTS \u003e CHEMICAL METERS/ANALYZERS \u003e FLUORESCENCE MICROSCOPY; IN SITU/LABORATORY INSTRUMENTS \u003e PHOTON/OPTICAL DETECTORS \u003e MICROSCOPES; IN SITU/LABORATORY INSTRUMENTS \u003e CHEMICAL METERS/ANALYZERS \u003e FLUOROMETERS; IN SITU/LABORATORY INSTRUMENTS \u003e SAMPLERS \u003e BOTTLES/FLASKS/JARS \u003e GO-FLO BOTTLES", "is_usap_dc": true, "keywords": "R/V NBP; B-15J", "locations": "B-15J", "north": -43.5676, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Jeffrey, Wade H.; Neale, Patrick", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repo": "USAP-DC", "repositories": "R2R; USAP-DC", "science_programs": null, "south": -77.8793, "title": "Collaborative Proposal: Interactive Effects of UV Radiation and Vertical Mixing on Phytoplankton and Bacterial Productivity of Ross See Phaeocystis Blooms", "uid": "p0000578", "west": -177.639}, {"awards": "0440609 Price, P. Buford", "bounds_geometry": "POINT(-112.06556 -79.469444)", "dataset_titles": null, "datasets": null, "date_created": "Tue, 03 Jun 2008 00:00:00 GMT", "description": "This award supports a project to use three downhole instruments - an optical logger; a\u003cbr/\u003eminiaturized biospectral logger at 420 nm (miniBSL-420); and an Acoustic TeleViewer (ATV) - to log a 350-m borehole at the WAIS Divide drill site. In addition, miniBSL-224 (at 224 nm) and miniBSL-420 will scan ice core sections at NICL to look for abrupt climate changes, volcanic ash, microbial concentrations, and correlations among them. Using the optical logger and ATV to log bubble number densities vs depth in a WAIS Divide borehole, we will detect annual layers, from which we can establish the age vs depth relation to the bottom of the borehole that will be available during the three-year grant period. With the same instruments we will search for long-period modulation of bubble and dust concentrations in order to provide definitive evidence for or against an effect of long-period variability of the sun or solar wind on climate. We will detect and accurately date ash layers in a WAIS Divide borehole. We will match them with ash layers that we previously detected in the Siple Dome borehole, and also match them with sulfate and ash layers found by others at Vostok, Dome Fuji, Dome C, and GISP2. The expected new data will allow us to extend our recent study which showed that the Antarctic record of volcanism correlates with abrupt climate change at a 95% to \u003e99.8% significance level and that the volcanic signatures at bipolar locations match at better than 3 sigma during the interval 2 to 45 kiloyears. The results to be obtained during this grant period will position us to extend an accurate age vs depth relation and volcano-climate correlations to earlier than 150 kiloyears ago in the future WAIS Divide borehole to be drilled to bedrock. Using the miniBSLs to identify biomolecules via their fluorescence, we will log a 350-m borehole at WAIS Divide, and we will scan selected lengths of ice core at NICL. Among the biomolecules the miniBSLs can identify will be chlorophyll, which will provide the first map of aerobic microbes in ice, and F420, which will provide the first map of methanogens in ice. We will collaborate with others in relating results from WAIS Divide and NICL ice cores to broader topics in climatology, volcanology, and microbial ecology. We will continue to give broad training to undergraduate and graduate students, to attract underrepresented minorities to science, engineering, and math, and to educate the press and college teachers. A deeper understanding of the causes of abrupt climate change, including a causal relationship with strong volcanic eruptions, can enable us to understand and mitigate adverse effects on climate.", "east": -112.06556, "geometry": "POINT(-112.06556 -79.469444)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e CHEMICAL METERS/ANALYZERS \u003e FLUORESCENCE SPECTROSCOPY; IN SITU/LABORATORY INSTRUMENTS \u003e RECORDERS/LOGGERS \u003e OPTICAL DUST LOGGERS", "is_usap_dc": false, "keywords": "Volcanic Ash; Dust Concentration; Antarctica; FIELD INVESTIGATION; Liquid Veins In Ice; Optical Logger; Borehole; Ash Layer; FIELD SURVEYS; Microbial Metabolism; Climate; Biospectral Logger; Not provided; Protein Fluorescence; Gas Artifacts; Aerosol Fluorescence; Volcanism; WAIS Divide; Ice Core", "locations": "WAIS Divide; Antarctica", "north": -79.469444, "nsf_funding_programs": "Antarctic Glaciology", "paleo_time": null, "persons": "Bay, Ryan; Price, Buford", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION; LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS; Not provided", "repositories": null, "science_programs": null, "south": -79.469444, "title": "Climatology, Volcanism, and Microbial Life in Ice with Downhole Loggers", "uid": "p0000746", "west": -112.06556}]
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Ecosystems worldwide are threatened by anthropogenic changes in climate. Lakes are widely regarded as sentinels of climate change and, among these, polar lakes are the most sensitive. Beneath meters of permanent ice and liquid water, many Antarctic lakes contain complex microbial communities that are already being transformed by climate change. The structurally complex spatial patterns that these microbes create provide the opportunity to pursue research questions about spatial ecology that cannot be addressed elsewhere. This project focuses on research that will advance understanding of the spatial structure of benthic communities in Antarctic lakes, their relationships with environmental conditions, and predictions for likely changes in the future. This project will also advance methods in integrating the morphology and spatial patterning of modern microbial communities in relationship to their biophysical and biochemical environments. The quantitative framework being developed has potential to refine understanding of controls on microbial community patterning and thus interpretation of both the effects of climate change and ancient fossil microbial communities in the geologic record. Such understanding will address key questions about Earth’s evolutionary and environmental history and future. Lake Vanda in the McMurdo Dry Valleys, Antarctic, has modern microbial pinnacles covering its lake floor. Using existing datasets on spatial structure of benthic communities from 37 sites on the floor of Lake Vanda, the project team will apply recent theories from Spatial Ecology to investigate the mechanisms that give rise to spatial patterns of pinnacles formed by benthic microbes. The work addresses two questions: (1) What are the morphological and spatial patterns of pinnacles and how do they vary over developmental stages, along environment gradients, and from 2013 to 2023? And (2) what mechanisms give rise to the geometry of individual pinnacles and their spatial distribution? Lake Vanda provides an exceptional opportunity to address these questions. It features well characterized gradients in sedimentation, nutrients, irradiance, transport mechanism, and colonization history. Benthic communities at different locations in the lake manifest distinct spatial patterns, as they experience distinct conditions. Lake level has increased >10 m in the past few decades, creating additional opportunities for a “natural experiment” on pattern development by comparing relatively newly flooded substrates (pinnacles of 1 to 15 years old) with deeper, well-developed mats (> 70 years old). Since microbial communities respond to environmental change rapidly, analyses can characterize changes in patterns in pinnacle spatial data collected 9 years apart (Dec 2013 and Jan 2023), providing the opportunity to directly assess responses of spatially self-organized ecosystems to environmental change. As such, Lake Vanda is a natural laboratory that allows research (1) to effectively sort out mechanisms of pattern formation affecting benthic microbial communities residing there; and (2) to test the theory of spatial self-organization: mechanisms of pattern formation and responses to perturbations, applicable to ecosystems worldwide. Research questions will be addressed by integrating existing datasets, spatial pattern analyses, Bayesian statistical models, and process-based numerical models. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
Non-technical description Marine invertebrates often have mutually beneficial partnerships with microorganisms that biosynthesize compounds with nutritive or defensive functions and are integral for survival. Additionally, these “natural products” often have bioactive properties with human health applications fighting infection or different types of cancer. This project focuses on the ascidian (“sea squirt”) Synoicum adareanum, found in the Anvers Island region of the Antarctic Peninsula, and was recently discovered to contain high levels of a natural product, palmerolide A (palA) in its tissues. The microorganism that produces palA is a new bacterial species, Candidatus Synoicihabitans palmerolidicus, found in a persistent partnership with the sea squirt. There is still much to be learned about the fundamental properties of this sea squirt-microbe-palA system including the geographical range of the animal-microbe partnership, its chemical and microbiome complexity and diversity, and the biological effect of palA in the sea squirt. To address these questions, this multidisciplinary research team will investigate the sea squirt-microbiome partnership in the Antarctic Peninsula and McMurdo Sound regions of the Ross Sea using a state-of-the-art strategy that will advance our understanding of the structural and functional features of the sea squirt and microbiome in detail, and reveal the roles that the palA natural product plays in the host ecology in its native Antarctic seafloor habitat. The project will broaden diversity and provide new opportunities for early career students and postdoctoral researchers to participate in field and laboratory-based research that builds an integrative understanding of Antarctic marine biology, ecology, physiology and chemistry. In addition, advancing the understanding of palA and its biological properties may be of future benefit to biomedicine and human health. Technical description Marine invertebrates and their associated microbiomes can produce bioactive natural products; in fact, >600 such compounds have been identified in species from polar waters. Although such compounds are typically hypothesized to serve ecological roles in host survival through deterring predation, fouling, and microbial infection, in most cases neither the producing organism nor the genome-encoded biosynthetic enzymes are known. This project will study an emerging biosynthetic system from a polar ascidian-microbe association that produces palA, a natural product with bioactivity against the proton-pumping enzyme V-type H+-ATPase (VHA). The objectives include: (i) Determining the microbiome composition, metabolome complexity, palA levels, and mitochondrial DNA sequence of S. adareanum morphotypes at sites in the Antarctic Peninsula and in McMurdo Sound, (ii) Characterizing the Synoicum microbiome using a multi-omics strategy, and (iii) Assessing the potential for co-occurrence of Ca. S. palmerolidicus-palA-VHA in host tissues, and (iv) exploring the role of palA in modulating VHA activity in vivo and its effects on ascidian-microbe ecophysiology. Through a coupled study of palA-producing and non-producing S. adareanum specimens, structural and functional features of the ascidian microbiome metagenome will be characterized to better understand the relationship between predicted secondary metabolite pathways and whether they are expressed in situ using a paired metatranscriptome sequencing and secondary metabolite detection strategy. Combined with tissue co-localization results, functional ecophysiological assays aim to determine the roles that the natural product plays in the host ecology in its native Antarctic seafloor habitat. The contributions of the project will inform this intimate host-microbial association in which the ascidian host bioaccumulates VHA-inhibiting palA, yet its geo-spatial distribution, cellular localization, ecological and physiological role(s) are not known. In addition to elucidating the ecophysiological roles of palA in their native ascidian-microbe association, the results will contribute to the success of translational science, which aligns with NSF’s interests in promoting basic research that leads to advances in Biotechnology and Bioeconomy. The project will also broaden diversity and provide new opportunities for early career students and postdoctoral researchers to participate in field and laboratory-based research that builds an integrative understanding of Antarctic marine biology, ecology, physiology and chemistry. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
The ozone hole that develops over the Antarctic continent every spring is one of the features attributed to human activity, in particular production of the CFC (chlorofluorocarbons in refrigerants) released to the atmosphere. In spite of the CFC ban from the Montreal Protocol established in the year 1987, the recovery has been slower than predicted. Bromocarbons, known to produce the stratospheric ozone depletion, have recently been estimated to contribute to the pool of bromines in the lower atmosphere. What is the origin of the bromocarbons in Antarctic sea ice? Is this an additional source of chemicals creating the ozone hole? This project will test if bromocarbons in sea ice are produced and degraded by microalgae and bacteria found in sea ice, in snow and the interface between the two. The project will collect a suite of chemical and biological measurements of sea ice and snow to determine bromocarbon concentrations, microbial activity associated with them, and intra-cellular genes and proteins involved in bromocarbon metabolism. This project benefits NSF’s goals of expanding fundamental knowledge of Antarctic systems, biota, and processes, and improving the understanding of interactions among the Antarctic systems, cryosphere and organisms. The work will be carried out at McMurdo Station in late austral spring, including sampling of snow and ice that will be concentrated in the laboratory, and 24-hour experiments to measure algal and bacterial activity. Genes controlling synthesis of enzymes involved in bromocarbon metabolism are of interest in biotechnology and bioremediation, including products that repair damaged skin from UltraViolet Radiation. The project will train undergraduate students on chemical and biological techniques. The Principal Investigators will be involved in the Pacific Science Center in Seattle with ~10,000 visitors per weekend where they will develop a project-specific exhibit. The microbial processes in snow and ice associated with bromocarbon synthesis and degradation have not been studied in Antarctica during winter and spring. This study will inform about microbial activity in relation to the release of bromocarbons compounds from the snow and ice surfaces, compounds known to degrade stratospheric ozone. The estimation of chemical bromocarbons will be combined with metagenomics and metaproteomics approaches for understanding the potential role of microbes in snow and sea ice. The environmental, chemical and biological data will be synthesized with multivariate analysis and significant differences between sites and experimental treatments with ANOVA. A collaborator from the University of Goteborg in Sweden will collaborate on bromocarbon analyses. The study will also address “saline snow” a new environment not previously studied for microbial life. In addition, this is the first study of meta-proteomics in snow and ice. The Principal Investigators expect their results will help inform ozone hole recovery in the 21st century. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
Perennially ice-covered lakes in the McMurdo Dry Valleys of Antarctica contain abundant microbial mats, and the export of this mat material can fertilize the surrounding polar desert ecosystems. These desert soils are one of the most organic-poor on earth yet host a community of microorganisms. Microbial mat material is exported from the shallow, gas-supersaturated regions of the lakes when gas bubbles form in the mats, lifting them to the ice cover; the perennial ice cover maintains gas supersaturation. These mats freeze in and are exported to the surrounding soils through ice ablation. The largest seasonal decrease and thinnest ice cover in the history of Lake Fryxell was recorded during the 2022-2023 Austral summer. In this thin ice year, the water column dissolved oxygen increased over prior observations, and the lake bottom surface area with bubble-disrupted mat was more than double that observed in 1980-1981 and 2006-2007. This work will constrain mat mobilization within and out of Lake Fryxell in the McMurdo Dry Valleys during a period of unprecedented ice thinning to understand how future changing regional climate and predicted seasonal loss of lake ice cover will affect nutrient transport in the McMurdo Dry Valleys. Exceptional years of mat export are hypothesized to have the most significant impact on nutrient export to soil communities; variability in mat liftoff may thus play a role in the McMurdo Dry Valleys ecosystem response to changing climate. The perennial ice cover of lakes in the McMurdo Dry Valleys of Antarctica modulates the transfer of gasses, organic and inorganic material, between the lakes and surrounding soils. The export of biomass in these lakes is driven by the supersaturation of atmospheric gasses in the shallow regions under perennial ice cover. Gas bubbles nucleate in the mats, producing buoyancy that lifts them to the bottom of the ice, where they freeze in and are exported to the surrounding soils through ice ablation. These mats represent a significant source of biomass and nutrients to the McMurdo Dry Valleys soils, which are among the most organic-poor on earth. Nevertheless, this biomass remains unaccounted for in organic carbon cycling models for the McMurdo Dry Valleys. Ice cover data from the McMurdo Dry Valleys Long Term Ecological Research Project shows that the ice thickness has undergone cyclical variation over the last 40 years, reaching the largest seasonal decrease and thinnest ice-cover in the recorded history of Lake Fryxell during the 2022-2023 austral summer. Preliminary work shows that the surface area with mat liftoff at Lake Fryxell is more than double that observed in 1980-1981 and 2006-2007, coinciding with this unprecedented thinning of the ice-cover and an increase in the water column dissolved O2. This research will constrain biomass mobilization within and out of Lake Fryxell in the McMurdo Dry Valleys during a period of unprecedented ice thinning. The researchers hypothesize that a thinner ice cover promotes more biomass mobilization by 1) stimulating additional production of gas bubbles from the existing gas-supersaturated waters during summertime photosynthesis to create microbial mat liftoff and 2) promoting mat liftoff in deeper, thicker microbial mats, and 3) that this biomass can be traced into the soils by characterizing its chemistry and modeling the most likely depositional settings. This work will use microbial mat samples, lake dissolved oxygen and photosynthetically active radiation data and underwater drone footage documenting the depth distribution of liftoff mats in January 2023, and long-term ice cover thickness, photosynthetically active radiation, and lake level change data collected by the McMurdo Dry Valleys Long Term Ecological Research Project to test hypotheses 1-3. The dispersal of the liftoff mat exposed at Lake Fryxell surface will be modeled using a Hybrid Single-Particle Lagrangian Integrated Trajectory (HYSPLIT) model. Exceptional liftoff years like the present are hypothesized to have the most significant impact on the soil communities as the rates of soil respiration increase with the addition of carbon. However, continued warming in the next 10 - 40 years may result in seasonal loss of the ice cover and cessation of liftoff mat export. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
The objective of this project is to understand why the nitrous oxide (N2O) content of the atmosphere was lower during the last ice age (about 20,000-100,000 years ago) than in the subsequent warm period (10,000 years ago to present) and why it fluctuated during climate changes within the ice age. Nitrous oxide is a greenhouse gas that contributes to modern global warming. It is thought that modern warming will in turn cause increases in natural sources of nitrous oxide from bacteria in soils and the ocean, creating a "positive feedback." However, the amount these sources will increase is uncertain because the different ways that nitrous oxide are produced, and how sensitive they are to warmer climate, are not well known. This project will measure a unique property of the nitrous oxide molecule in very large ancient air samples from a glacier in Antarctica. This method can distinguish between different microbial processes that produce nitrous oxide but it has not been applied yet to the time periods in question. The data will provide information about how natural climate changes affect nitrous oxide production. This, in turn, will be useful for predicting future changes and for understanding why the Earth's climate shifts from ice ages to warm periods and back again. Ice-core records of greenhouse gas isotopic composition are useful for determining past changes in natural source and sink strengths and for understanding how natural emissions are linked to climate change. This project will develop two records of the intramolecular site preference of Nitrogen-15 in N2O. One record spans the last deglaciation (10,000-21,000 years ago) when atmospheric N2O concentration rose by 30 percent, and the other record spans millennial-scale climate changes during the last ice age when N2O varied by smaller amounts (Heinrich Stadial 4 and Dansgaard Oeschger 8, 35,000-41,000 years ago). The records will be used to understand what changes in the nitrogen cycle caused atmospheric N2O concentration to vary and what mechanisms link the N2O emissions to climate change. Ideally, studying the two different time periods will isolate the millennial climate responses entangled with the full deglacial sequence, creating a clearer picture of how N2O biogeochemistry responds to climate change. This work will also allow exploration of an isotopic tracer for in situ production of N2O that contaminates the atmospheric signal in particularly dusty ice. The project will use a unique, well-dated suite of ice samples from Taylor Glacier, Antarctica and continuous flow isotope ratio mass spectrometry on a custom gas extraction line operated in the Oregon State University laboratory. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
Couradeau, Estelle; Maximova, Siela; Machado, Jose Luis
No dataset link provided
Páramos are high-altitude tundra ecosystems nested at the heart of the Andes mountains. These cold and humid environments are home to a multitude of plants, animals, and insects. Páramos are a critical water source for downstream urban centers, including Colombia's capital city, Bogota. Additionally, the Páramos soils contain substantial organic carbon reserves due to slow rates of organic matter decomposition. Beyond being a pool of carbon sequestered away from the atmosphere, this large reservoir of organic matter controls the soils’ hydraulic and fertility properties. The Páramos’ unique geographic location, at an elevation above 2,800 m above sea level, makes them highly vulnerable to the impacts of climate change. In fact, these ecosystems’ surface areas are projected to shrink by half within the next 50 years possibly causing loss of the essential services they provide. This project aims to characterize the microbial diversity in the Páramos soils in Colombia and investigate how climate change will affect microbes’ functions. The research is of high importance, considering that immediate and long-term changes in microbial metabolism could impact the ability of Páramos soils to store organic carbon and regulate downstream water flow. To study the cascading effect of climate change on Páramos ecosystems, this project will jumpstart collaborations among transdisciplinary experts that will integrate the research of below-ground microbial communities with above-ground vegetation functions. The project will also engage high school and undergraduate students that will work together to develop and deploy low-cost long-term soil monitoring data loggers in Chingaza National Natural Park, near the city of Bogota. This project will address the critical need to disentangle the effect of moisture and temperature on the fate of organic carbon in Páramos soils while building a transdisciplinary team capable of expanding the scope of the research to an ecosystem level. The project includes establishing controlled soil mesocosms that will allow to independently vary moisture and temperature levels. Additionally, functions of the soil microbiome will be investigated using metagenomics and amplicon sequencing, and probes will be deployed to initiate long-term monitoring of the soil response to climate change in situ. This project will culminate in the organization of an international Páramos symposium that will set up priorities for future systems research. The symposium will bring together scientists from diverse fields to discuss the linkages between above-ground and below-ground ecosystem functions and plan future collaborations in predicting Páramos-wide effects of climate change. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
Non-technical Abstract The McMurdo Dry Valleys LTER seeks to understand how changes in the temporal variability of ecological connectivity interact with existing landscape legacies to alter the structure and functioning of this extreme polar desert ecosystem. This research has broad implications, as it will help us to understand how natural ecosystems respond to ongoing anthropogenic global change. At the same time, this project also serves an important educational and outreach function, providing immersive research and educational experiences to students and artists from diverse backgrounds, and helping to ensure a diverse and well-trained next generation of leaders in polar ecosystem science and stewardship. Ultimately, the results of this project will help us to better understand and prepare for the effects of climate change and develop scientific insights that are relevant far beyond Antarctic ecosystems. The McMurdo Dry Valleys (MDVs) make up an extreme polar desert ecosystem in the largest ice-free region of Antarctica. The organisms in this ecosystem are generally small. Bacteria, microinvertebrates, cyanobacterial mats, and phytoplankton can be found across the streams, soils, glaciers, and ice-covered lakes. These organisms have adapted to the cold and arid conditions that prevail outside of lakes for all but a brief period in the austral summer when the ecosystem is connected by liquid water. In the summer when air temperatures rise barely above freezing, soils warm and glacial meltwater flows through streams into the open moats of lakes. Most biological activity across the landscape occurs in summer. Through the winter, or polar night (6 months of darkness), glaciers, streams, and soil biota are inactive until sufficient light, heat, and liquid water return, while lake communities remain active all year. Over the past 30 years, the MDVs have been disturbed by cooling, heatwaves, floods, rising lake levels, as well as permafrost and lake ice thaw. Considering the clear ecological responses to this variation in physical drivers, and climate models predicting further warming and more precipitation, the MDV ecosystem sits at a threshold between the current extreme cold and dry conditions and an uncertain future. This project seeks to determine how important the legacy of past events and conditions versus current physical and biological interactions shape the current ecosystem. Four hypotheses will be tested, related to 1) whether the status of specific organisms are indicative ecosystem stability, 2) the relationship between legacies of past events to current ecosystem resilience (resistance to big changes), 3) carryover of materials between times of high ecosystem connectivity and activity help to maintain ecosystem stability, and 4) changes in disturbances affect how this ecosystem persists through the annual polar night (i.e., extended period of dark and cold). Technical Abstract In this iteration of the McMurdo LTER project (MCM6), the project team will test ecological connectivity and stability theory in a system subject to strong physical drivers (geological legacies, extreme seasonality, and contemporary climate change) and driven by microbial organisms. Since microorganisms regulate most of the world’s critical biogeochemical functions, these insights will be relevant far beyond polar ecosystems and will inform understanding and expectations of how natural and managed ecosystems respond to ongoing anthropogenic global change. MCM6 builds on previous foundational research, both in Antarctica and within the LTER network, to consider the temporal aspects of connectivity and how it relates to ecosystem stability. The project will examine how changes in the temporal variability of ecological connectivity interact with the legacies of the existing landscape that have defined habitats and biogeochemical cycling for millennia. The project team hypothesizes that the structure and functioning of the MDV ecosystem is dependent upon legacies and the contemporary frequency, duration, and magnitude of ecological connectivity. This hypothesis will be tested with new and continuing monitoring, experiments, and analyses of long-term datasets to examine: 1) the stability of these ecosystems as reflected by sentinel taxa, 2) the relationship between ecological legacies and ecosystem resilience, 3) the importance of material carryover during periods of low connectivity to maintaining biological activity and community stability, and 4) how changes in disturbance dynamics disrupt ecological cycles through the polar night. Tests of these hypotheses will occur in field and modeling activities using new and long-term datasets already collected. New datasets resulting from field activities will be made freely available via widely-known online databases (MCM LTER and EDI). The project team has also developed six Antarctic Core Ideas that encompass themes from data literacy to polar food webs and form a consistent thread across the education and outreach activities. Building on past success, collaborations will be established with teachers and artists embedded within the science teams, who will work to develop educational modules with science content informed by direct experience and artistic expression. Undergraduate mentoring efforts will incorporate computational methods through a new data-intensive scientific training program for MCM REU students. The project will also establish an Antarctic Research Experience for Community College Students at CU Boulder, to provide an immersive educational and research experience for students from diverse backgrounds in community colleges. MCM LTER will continue its mission of training and mentoring students, postdocs, and early career scientists as the next generation of leaders in polar ecosystem science and stewardship. Historically underrepresented participation will be expanded at each level of the project. To aid in these efforts, the project has established Education & Outreach and Diversity, Equity, and Inclusion committees to lead, coordinate, support, and integrate these activities through all aspects of MCM6. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
Sea ice in Antarctic coastal waters shape ecosystems, both in the surface waters and at the bottom of the ocean, environments that depend on algae living in sea ice for their productivity. With high variability in sea ice formation and melt between years and as a response to climate change, it is of importance to obtain better understanding of the interaction of sea ice with algae, as well as provide better data for global climate models. This project will accomplish those goals by measuring phytoplankton growth and cellular properties in sea ice with experiments performed using an ice tank. Laboratory experiments will be based on previous observations in the Antarctic Peninsula coastal waters, providing realistic conditions to emulate. The scientific importance of the proposed work aligns with the National Science Foundation goals to understand the biological and chemical properties of sea ice bio-geo-chemistry and its feedbacks with seasonal sea ice dynamics and climate. The finding from this project will be of interest to a broad scientific community, including oceanographers, biologists, chemists, and ecosystem and ocean modelers. To address the scarcity of data on sea ice microbes that limits our ability to predict future Antarctic climate with accuracy, the principal investigator will develop an Antarctic Science Minor in order to train future scientists with an environmental perspective and prepare the future US workforce with a strong scientific background on Earth and Biological Sciences. There is a paucity of data to understand the processes underlying observed patters in sea ice quality and their interaction with the sea-ice microbial community. This project will provide a mechanistic understanding of primary production and physiology of sympagic algae over the seasonal cycle of formation and melt of Antarctic sea ice. Although sea ice is central to the Antarctic coastal ecosystems, little is known of how they affect, and are in turn affected, by sea-ice algae. This project concentrates on first-year sea ice, forming and melting each year, creating unique and very dynamic habitats. The study will be structured by 4 main objectives: 1) how different algal species adapt to the seasonal changes in sea ice conditions, 2) how different methods to measure primary production (carbon dioxide drawdown, oxygen production and variable fluorescence) relate in sea ice and differ from sea water measurements, 3) how sympagic algae influence the physical structure of sea ice, 4) how sympagic algae contribute to organic matter cycling during ice melt. Due to expected changes in sea ice due to climate change, this study is uniquely positioned to provide needed data on short-term and seasonal processes. Results from this study will be useful to refine models of algal production in Antarctic and Arctic ecosystems, data not available to date as sea ice and its biogeochemistry are often poorly represented in earth system models. This project will also provide education for graduate and undergraduate students as well as material to develop class curriculum for middle-school students. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
Ice cores from glaciers and ice sheets provide detailed archives of past environmental conditions, furthering our understanding of Earth’s climate. Microorganisms in the West Antarctic Ice Sheet are buried over glaciological time and form a stratigraphy record providing the opportunity of analysis of the order and position of layers of geological events, with potential links to Southern Hemisphere climate. However, microbial cells that land on the ice sheet are subject to the stresses of changing habitat conditions due to burial and conditions associated with long-term isolation in ice. These processes may lead to a loss of fidelity within the stratigraphic record of microbial cells. We know little about how and if microorganisms survive burial and remain alive over glacial-interglacial time periods within an ice sheet. This analysis will identify the viable and preserved community of microorganisms and core genomic adaptation that permit cell viability, which will advance knowledge in the areas of microbiology and glaciology while increasing fidelity of ice core measurements relevant to past climate and potential future global climate impacts. This exploratory endeavor has the potential to be a transformative step toward understanding the ecology of one of the most understudied environments on Earth. The project will partner with the Museum of Science, Boston, to increase public scientific literacy via education and outreach. Additionally, this project will support two early-career scientists and two undergraduates in interdisciplinary research at the intersection of microbiology and climate science. Results from this project will provide the first DNA data based on single-cell whole genomic sequencing from the Antarctic Ice Sheet and inform whether post-depositional processes impact the interpretations of paleoenvironmental conditions from microbes. The goals to determine the taxonomic identity of viable and preserved microbial cells, and decode the genetic repertoire that confers survival of burial and long-term viability within glacial ice, will be achieved by utilizing subsamples from a ~60,000 year old record of the West Antarctic Ice Sheet Divide (WD) Ice Core. WD samples will be melted using the Desert Research Institute’s ice core melting system that is optimized for glaciobiological sampling. Microbial cells from the meltwater will be sorted using fluorescence-activated cell sorting, and individually sorted cells will have their genomes sequenced. The fluorescence-based methods will discern the viable (metabolically active) cells from those cells that are non-viable but preserved in the ice (DNA-containing). The genomic analysis will identify the taxonomy of each cell, presence of known genes that confer survival in permanently frozen environments, and comparatively analyze genomes to determine the core set of genes required by viable cells to persist in an ice sheet. The outcomes of this work will expand the potential for biological measurements and contamination control from archived ice cores. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
Varsani, Arvind; Porazinska, Dorota; Schmidt, Steven; Bergstrom, Anna
No dataset link provided
Cryoconite holes are sediment-filled melt holes in the surface of glaciers that can be important sites of active microbial life in an otherwise mostly frozen and barren landscape. Previous studies in the McMurdo Dry Valleys, Antarctica suggest that viral infections of microbes, and a general lack of fertilizers (i.e., nutrients), may be important factors shaping the development and functioning of microbial communities in cryoconite holes. The researchers propose an experimental approach to understand how nutrient limitation affects diversity (number of species) and overall abundance of microbes, and how the diversity and abundance of microbes in turn affects the diversity, abundance, and infection type of viruses that parasitize the microbes in cryoconite sediments. The researchers will use sediments previously collected from Antarctic glaciers that have varying concentrations of viruses and nutrients, to set up a nutrient-addition experiment to determine how nutrients affect microbial and viral population dynamics. The results will deepen our understanding of how microbial communities in general are shaped by nutrients and viruses and give new insights into the functioning of viruses in extremely cold environments. The researchers will publish their findings in scientific journals and will share their discoveries with K-12 students from rural schools in collaboration with the Pinhead Institute and will connect undergraduate students from under-represented minorities to polar research through participation in the university’s Science, Technology, Engineering & Mathematics Routes Uplift Research Program. Outreach will be achieved through videos produced and distributed by a professional science communicator. The research advances a National Science Foundation goal of expanding fundamental knowledge of Antarctic systems, biota, and processes by utilizing the unique characteristics of the Antarctic region as a science observing platform. The Principal Investigators propose an experimental approach to understand how nutrient limitation affects microbial diversity and abundances and their cascading effects on virus diversity, abundance, and mode of infection (lysis vs. lysogeny) in Antarctic cryoconite holes. Cryoconite holes are ideal natural microcosms for manipulative studies, not available in other cryospheric ecosystems. The PIs will use previously collected cryoconite from across a gradient of both viral diversity and nutrient levels to address questions about key limiting nutrients and microbial-viral community dynamics in cryoconite sediments. Nutrient manipulation experiments will be conducted in a growth chamber that closely approximates the light and temperature regime of in situ cryoconite holes to test three core hypotheses: (1) phosphorus availability limits microbial productivity and abundance in cryoconite holes; (2) relaxing nutrient limitation in cryoconite from low-diversity glaciers will increase species diversity, leading microbial communities to resemble those found on more nutrient-rich glaciers; (3) relaxing nutrient limitation will increase the diversity and abundance of viruses by increasing the availability of suitable hosts, and decrease the prevalence of lysogenic infections. By manipulating nutrient limitation within a realistic range, this project will help verify hypothesized phosphorus limitation of Antarctic cryoconite holes and will extend understanding of the connections between nutrients, diversity, and viral infection dynamics in the cryosphere more generally. A better understanding of these dynamics in cryoconite sediments improves the ability of scientists to forecast future impacts of environmental changes in the cryosphere. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
Not all of Antarctica is covered in ice. In fact, soils are common to many parts of Antarctica, and these soils are often unlike any others found on Earth. Antarctic soils harbor unique microorganisms able to cope with the extremely cold and dry conditions common to much of the continent. For decades, microbiologists have been drawn to the unique soils in Antarctica, yet critical knowledge gaps remain. Most notably, it is unclear what properties allow certain microbes to thrive in Antarctic soils. By using a range of methods, this project is developing comprehensive model that discovers the unique genomic features of soils diversity, distributions, and adaptations that allow Antarctic soil microbes to thrive in extreme environments. The proposed work will be relevant to researchers in many fields, including engineers seeking to develop new biotechnologies, ecologists studying the contributions of these microbial communities to the functioning of Antarctic ecosystems, microbiologists studying novel microbial adaptations to extreme environmental conditions, and even astrobiologists studying the potential for life on Mars. More generally, the proposed research presents an opportunity to advance our current understanding of the microbial life found in one of the more distinctive microbial habitats on Earth, a habitat that is inaccessible to many scientists and a habitat that is increasingly under threat from climate change. The research project explores the microbial diversity in Antarctic soils and links specific features to different soil types and environmental conditions. The overarching questions include: What microbial taxa are found in a variety of Antarctic environments? What are the environmental preferences of specific taxa or lineages? What are the genomic and phenotypic traits of microorganisms that allow them to persist in extreme environments and determine biogeographical differneces? This project will analyze archived soils collected from across Antarctica by a network of international collaborators, with samples selected to span broad gradients in soil and site conditions. The project uses cultivation-independent, high-throughput genomic analysis methods and cultivation-dependent approaches to analyze bacterial and fungal communities in soil samples. The results will be used to predict the distributions of specific taxa and lineages, obtain genomic information for the more ubiquitous and abundant taxa, and quantify growth responses in vitro across gradients in temperature, moisture, and salinity. This integration of ecological, environmental, genomic, and trait-based information will provide a comprehensive understanding of microbial life in Antarctic soils. This project will also help facilitate new collaborations between scientists across the globe while providing undergraduate students with 'hands-on' research experiences that introduce the next generation of scientists to the field of Antarctic biology. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
Glacial ice cores serve as a museum back in time, providing detailed records of past climatic conditions. In addition to chronological records such as temperature, chemistry and gas composition, ice provides a unique environment for preserving microbes and other biological materials through time. These microbes provide invaluable insight into the physiological capabilities necessary for survival in the Earth’s cryosphere and other icy planetary bodies, yet little is known about them. This award supports fundamental research into the activity of microbes in ice, and directly supports major research priorities regarding Antarctic biota identified in the 2015 National Academies of Sciences, Engineering, and Medicine report, A Strategic Vision for NSF Investments in Antarctic and Southern Ocean Research. The broader impacts of this work are that it will be relevant to researchers across paleoclimate and biological fields. It will support two early career researchers, a graduate and an undergraduate student who will conduct laboratory analyses, participate in outreach activities, publish papers in scientific journals and present at conferences. This work will use previously collected ice cores to investigate englacial microbial activity from the Holocene back to the Last Glacial Maximum from the blue ice area of Taylor Glacier, Antarctica. The proposal identified making significant contributions to 1) investing how Antarctic organisms evolve and adapt to changing environment, 2) understanding how microbes alter the preservation of paleorecord-relevant gas and trace element information in ice cores, and 3) identifying microbial life in cores and their activity in relation to dust depositional events. Two recently developed complementary techniques (bio-orthogonal noncanonical amino acid tagging and deuterium isotope probing) in combination with Raman Confocal Microspectroscopy will be used to assess and quantify microbial activity in ice. During phase one of the project, these methods will be optimized using deaccessioned ice cores available at the National Science Foundation’s Ice Core Facility. In phase two, ice cores in a time series from the Taylor Glacier will be analyzed for geochemistry and microbial activity. Research results will provide a comprehensive view of englacial microbial communities, including their metabolic diversity and activity, and the effect of geochemical parameters on microbial assemblages from different climate periods. Given the dearth of information available on englacial microbial communities, the results of this research will be of particular significance. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
Non-technical Abstract: The long isolation and unique biodiversity of the Southern Ocean represents an important case study region for understanding the evolution and ecology of populations. This study uses modern -omics approaches to evaluate the biodiversity, evolution, and ecology of Antarctic marine nematodes and their host-associated microbiomes from a variety of habitats collected at different depths. The results are producing an important baseline dataset of Antarctic meiofaunal diversity. All genomic resources generated in this project will be publicly accessible as open-source datasets with the potential for long-term scientific reuse. This project supports diverse researchers from underrepresented backgrounds and produces a suite of Antarctic-focused digital public outreach products. Technical Abstract: Nematode worms are abundant and ubiquitous in marine sediment habitats worldwide, performing key functions such as nutrient cycling and sediment stability. However, study of this phylum suffers from a perpetual and severe taxonomic deficit, with less than 5,000 formally described marine species. Fauna from the Southern Ocean are especially poorly studied due to limited sampling and the general inaccessibility of the Antarctic benthos. This study is providing the first large-scale molecular-based investigation from marine nematodes in the Eastern Antarctic continental shelf, providing an important comparative dataset for the existing body of historical (morphological) taxonomic studies. This project uses a combination of classical taxonomy (microscopy) and modern -omics tools to achieve three overarching aims: 1) determine if molecular data supports high biodiversity and endemism of benthic meiofauna in Antarctic benthic ecosystems; 2) determine the proportion of marine nematode species that have a deep-sea versus shallow-water evolutionary origin on the Antarctic shelf, and assess patterns of cryptic speciation in the Southern Ocean; and 3) determine the most important drivers of the host-associated microbiome in Antarctic marine nematodes. This project is designed to rapidly advance knowledge of the evolutionary origins of Antarctic meiofauna, provide insight on population-level patterns within key indicator genera, and elucidate the potential ecological and environmental factors which may influence microbiome patterns. Broader Impacts activities include an intensive cruise- and land-based outreach program focusing on social media engagement and digital outreach products, raising awareness of Antarctic marine ecosystems and understudied microbial-animal relationships. The diverse research team includes female scientists, first-generation college students, and Latinx trainees. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
Microbes in Antarctic surface marine sediments have an important role in degrading organic matter and releasing nutrients to the ocean. Organic matter degradation is at the center of the carbon cycle in the ocean, providing valuable information on nutrient recycling, food availability to animals and carbon dioxide release to the atmosphere. The functionality of these microbes has been inferred by their genomics, however these methods only address the possible function, not their actual rates. In this project the PIs plan to combine genomics methods with cellular estimates of enzyme abundance and activity as a way to determine the rates of carbon degradation. This project aims to sample in several regions of Antarctica to provide a large-scale picture of the processes under study and understand the importance of microbial community composition and environmental factors, such as primary productivity, have on microbial activity. The proposed work will combine research tools such as metagenomics, meta-transcriptomics, and metabolomics coupled with chemical data and enzyme assays to establish degradation of organic matter in Antarctic sediments. This project benefits NSFs goals of understanding the adaptation of Antarctic organisms to the cold and isolated environment, critical to predict effects of climate change to polar organisms, as well as contribute to our knowledge of how Antarctic organisms have adapted to this environment. Society will benefit from this project by education of 2 graduate students, undergraduates and K-12 students as well as increase public literacy through short videos production shared in YouTube. The PIs propose to advance understanding of polar microbial community function, by measuring enzyme and gene function of complex organic matter degradation in several ocean regions, providing a circum-Antarctic description of sediment processes. Two hypotheses are proposed. The first hypothesis states that many genes for the degradation of complex organic matter will be shared in sediments throughout a sampling transect and that where variations in gene content occur, it will reflect differences in the quantity and quality of organic matter, not regional variability. The second hypothesis states that a fraction of gene transcripts for organic matter degradation will not result in measurable enzyme activity due to post-translational modification or rapid degradation of the enzymes. The PIs will analyze sediment cores already collected in a 2020 cruise to the western Antarctic Peninsula with the additional request of participating in a cruise in 2023 to East Antarctica. The PIs will analyze sediments for metagenomics, meta-transcriptomics, and metabolomics coupled with geochemical data and enzyme assays to establish microbial degradation of complex organic matter in Antarctic sediments. Organic carbon concentrations and content in sediments will be measured with δ13C, δ15N, TOC porewater fluorescence in bulk organic carbon. Combined with determination of geographical variability as well as dependence on carbon sources, results from this study could provide the basis for new hypotheses on how climate variability, with increased water temperature, affects geochemistry in the Southern Ocean. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
Part I: Non-technical description: Microbial communities are of more than just a scientific curiosity. Microbes represent the single largest source of evolutionary and biochemical diversity on the planet. They are the major agents for cycling carbon, nitrogen, phosphorus, and other elements through the ecosystem. Despite their importance in ecosystem function, microbes are still generally overlooked in food web models and nutrient cycles. Moreover, microbes do not live in isolation: their growth and metabolism are influenced by complex interactions with other microorganisms. This project will focus on the ecology, activity and roles of microbial communities in Antarctic Lake ecosystems. The team will characterize the genetic underpinnings of microbial interactions and the influence of environmental gradients (e.g. light, nutrients, oxygen, sulfur) and seasons (e.g. summer vs. winter) on microbial networks in Lake Fryxell and Lake Bonney in the Taylor Valley within the McMurdo Dry Valley region. Finally, the project furthers the NSF goals of training new generations of scientists by including undergraduate and graduate students, a postdoctoral researcher and a middle school teacher in both lab and field research activities. This partnership will involve a number of other outreach training activities, including visits to classrooms and community events, participation in social media platforms, and webinars. Part II: Technical description: Ecosystem function in the extreme Antarctic Dry Valleys ecosystem is dependent on complex biogeochemical interactions between physiochemical environmental factors (e.g. light, nutrients, oxygen, sulfur), time of year (e.g. summer vs. winter) and microbes. Microbial network complexity can vary in relation to specific abiotic factors, which has important implications on the fragility and resilience of ecosystems under threat of environmental change. This project will evaluate the influence of biogeochemical factors on microbial interactions and network complexity in two Antarctic ice-covered lakes. The study will be structured by three main objectives: 1) infer positive and negative interactions from rich spatial and temporal datasets and investigate the influence of biogeochemical gradients on microbial network complexity using a variety of molecular approaches; 2) directly observe interactions among microbial eukaryotes and their partners using flow cytometry, single-cell sorting and microscopy; and 3) develop metabolic models of specific interactions using metagenomics. Outcomes from amplicon sequencing, meta-omics, and single-cell genomic approaches will be integrated to map specific microbial network complexity and define the role of interactions and metabolic activity onto trends in limnological biogeochemistry in different seasons. These studies will be essential to determine the relationship between network complexity and future climate conditions. Undergraduate researchers will be recruited from both an REU program with a track record of attracting underrepresented minorities and two minority-serving institutions. To further increase polar literacy training and educational impacts, the field team will include a teacher as part of a collaboration with the successful NSF-funded PolarTREC program and participation in activities designed for public outreach. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
Traditional models of oceanic food chains have consisted of photosynthetic algae (phytoplankton) being ingested by small animals (zooplankton), which were ingested by larger animals (fish). These traditional models changed as new methods allowed recognition of the importance of bacteria and other non-photosynthetic protozoa in more complex food webs. More recently, the wide-spread existence of mixotrophs (organisms that can both photosynthesize and ingest food particles) and their importance as microbial predators has been recognized in many oceanographic areas. In the Southern Ocean, the only two surveys of mixotrophs have suggested that there may be seasonal differences in their importance as predators. During the long polar night (winter), the ability of mixotrophs to ingest particulate food may aid in their survival thus ensuring a sufficient population in spring to support a phytoplankton bloom once photosynthesis rates can increase. Thus mixotrophs may provide a critical early food source upon which zooplankton and larger animals depend on for growth and reproduction. This project will advance understanding of mixotroph diversity and their ecological impact within the Southern Ocean microbial food web. Specifically, efforts will be focused on mixotrophy in the western Antarctica peninsula region during the austral spring and autumn when there are likely to be changes in the relative importance of photosynthesis and ingestion to mixotrophs. The project will provide research opportunities for undergraduate and graduate students and a post-doctoral researcher. There will be real-time outreach from the Southern Ocean to the public via blogs and interviews, and to high school art students through an established program that blends science and art education. Despite traditional views of protists as either "phototrophic" or "heterotrophic," there are many photosynthetic protists that consume prey (mixotrophy). Mixotrophy is a widespread phenomenon in aquatic systems and phytoplankton groups with known mixotrophic species, notably chrysophytes, cryptophytes, prymnesiophytes, prasinophytes and dinoflagellates, are present and often abundant in Antarctic waters. However, in the Southern Ocean, the presence of mixotrophic phytoflagellates has been surveyed only twice: in the Ross Sea during Austral spring 2008 and summer 2011. The primary goals of the project are to gain better understanding of mixotroph diversity and their ecological impact with respect to the Southern Ocean microbial food web. The contribution of mixotrophs to primary production and bacterial consumption is likely linked to the taxonomic composition of the community and the abundance of particular species. Abundances of novel mixotrophic species will be evaluated via qPCR, which will be coupled with assessments of rates of feeding and photosynthesis with the goal of describing how active mixotrophs direct the movement of carbon through food webs. These experiments will help the determination of how viable and widespread mixotrophy is as a nutritional strategy in polar waters and give direct information on the currently unknown diversity of mixotrophic taxa under different environmental conditions occurring in austral spring and autumn. Furthermore, the methods will simultaneously yield information on the whole communities of protists - mixotrophic, phototrophic and heterotrophic. In addition, a method to examine aspects of the taxonomic and functional diversities of the bacterivorous/mixotrophic community will be employed. A thymidine analog (BrdU) will be used to label DNA of eukaryotes feeding on bacteria. The BrdU-labeled eukaryotic DNA will be isolated using immunoprecipitation. High-throughput sequencing of the labeled DNA (bacterivores) versus unlabeled community DNA will determine the diversity of bacterivorous mixotrophs relative to other microeukaryotes. Flow cytometric sorting based on chlorophyll to focus on mixotrophic species. These approaches will elucidate a gap in current knowledge of the influence of microbial interactions in the Southern Ocean under different conditions. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
Part 1: Non-technical description: It is well known that the Southern Ocean plays an important role in global carbon cycling and also receives a disproportionately large influence of climate change. The role of marine viruses on ocean productivity is largely understudied, especially in this global region. This team proposes to use combination of genomics, flow cytometry, and network modeling to test the hypothesis that viral biogeography, infection networks, and viral impacts on microbial metabolism can explain variations in net community production (NCP) and carbon cycling in the Southern Ocean. The project includes the training of a postdoctoral scholar, graduate students and undergraduate students. It also includes the development of a new Polar Sci ReachOut program in partnership with the University of Michigan Museum of Natural History especially targeted to middle-school students and teachers and the general public. The team will also produce a Science for Tomorrow (SFT) program for use in middle schools in metro-Detroit communities and lead a summer Research Experience for Teachers (RET) fellows. Part 2: Technical description: The study will leverage hundreds of existing samples collected for microbes and viruses from the Antarctic Circumpolar Expedition (ACE). These samples provide the first contiguous survey of viral diversity and microbial communities around Antarctica. Viral networks are being studied in the context of biogeochemical data to model community networks and predict net community production (NCP), which will provide a way to evaluate the role of viruses in Southern Ocean carbon cycling. Using cutting edge molecular and flow cytometry approaches, this project addresses the following questions: 1) How/why are Southern Ocean viral populations distributed across environmental gradients? 2a) Do viruses interfere with "keystone" metabolic pathways and biogeochemical processes of microbial communities in the Southern Ocean? 2b) Does nutrient availability or other environmental variables drive changes in virus-microbe infection networks in the Southern Ocean? Results will be used to develop and evaluate generative models of NCP predictions that incorporate the importance of viral traits and virus-host interactions. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
The Antarctic Peninsula is experiencing rapid environmental changes, which will influence the community of organisms that live there. However, we know very little about the microscopic organisms living in the soil in this region. Soil biology (including bacteria, fungi, and invertebrates) are responsible for many important processes that sustain ecosystems, such as nutrient recycling. Without understanding the environmental conditions that influence soil biodiversity along the Antarctic Peninsula, our ability to predict the consequences of global change is strongly limited. This project will identify the soil community at many sites along the Antarctic Peninsula to discover how the community changes with environmental conditions from north to south. The project will also identify how the soil community at each site differs under different types of plants. Understanding more about the ways in which plant cover and climate conditions influence soil biodiversity will allow predictions of how communities will respond to future changes such as climate warming and invasive plant species. The project will also further the NSF goals of making scientific discoveries available to the general public and of training new generations of scientists. The investigators will engage with outreach to K-12 students and the general public both directly and through a blog and will participate in workshops for K-12 teachers. Additionally, the project will provide the opportunity for many undergraduate and graduate students of diverse backgrounds to be trained in interdisciplinary research. The investigators will determine the nature and strength of plant-soil linkages in influencing soil community composition and diversity over a latitudinal gradient of environmental and climatic conditions. The goals are to (1) increase our understanding of current biogeography and diversity by providing in-depth knowledge of soil community composition and complexity as it relates to environmental and climatic characteristics; and (2) determine the nature of aboveground-belowground community linkages over varying spatial scales. The team will identify the composition and diversity of soil communities under key habitat types (grass, moss, algae, etc.). Microbial communities (bacteria, fungi, archaea) will be investigated using pyrosequencing for community composition analysis and metagenomic sequencing to identify functional capabilities. Invertebrates (nematodes, tardigrades, rotifers, microarthropods) will be extracted and identified to the lowest possible taxonomic level. Soil chemistry (pH, nutrient content, soil moisture, etc.) and climate conditions will be measured to determine the relationship between soil communities and physical and chemical properties. Structural equation modeling will be used to identify aboveground-belowground linkage pathways and quantify link strengths under varying environmental conditions.
Part I: Non-technical summary The Antarctic Peninsula warmed very rapidly in the late part of the 20th century, much faster than the global average, and this warming is predicted to resume and continue over the rest of the 21st century. One consequence of this rapid warming is the melting and subsequent retreat of glaciers, leading to an increase in newly-exposed land on the Peninsula that was previously covered with ice. Once new terrain is exposed, the process of ecological succession begins, with the arrival of early-colonizing plants, such as moss and lichens, and soil organisms - a process commonly referred to as the “greening” of Antarctica. Early stages of succession will be an increasingly common feature on the Antarctic Peninsula, but the mechanisms by which they occur on the Antarctic continent is not well understood. Once the plants have established on the newly-exposed soil, they can change many important properties, such as water dynamics, nutrient recycling, soil development, and habitat for microscopic organisms, which will ultimately determine the structure and functioning of the future ecosystem as it develops. These relationships between vegetation, soil, and the associated microorganisms, referred to as “plant-soil” interactions, are something we know virtually nothing about. This project will be the first to make a comprehensive study of how the type of colonizing plant, and the expansion of those plants from climate change, will influence terrestrial ecosystems in Antarctica. Understanding these processes is critical to understanding how the greening Antarctica is occurring and how soil communities and processes are influenced by these expanding plant communities. Through this work the research team, will also be intensively training undergraduate and graduate students, including training of students from underrepresented groups and collaborative training of students from Chile and the US. Additionally, the research groups will continue their focus on scientific outreach with K-12 schools and the general public to expand awareness of the effects of climate change in Antarctica. Part II: Technical summary In this study, the researchers will use surveys across succession sites along the Antarctic Peninsula and Scotia Arc as well as a manipulative field experiment at glacier succession sites to test how species-specific plant functional traits impact soil properties and associated microbial and invertebrate communities in a greening Antarctica. In doing so, they will pursue three integrated aims to understand how Antarctic plant functional traits alter their soil environment and soil communities during succession after glacial retreat. AIM 1) Characterize six fundamental plant functional traits (thermal conductivity, water holding capacity, albedo, decomposability, tissue nutrient content, and secondary chemistry) among diverse Antarctica flora; AIM 2) Measure the relative effects of fundamental plant functional traits on soil physical properties and soil biogeochemistry across glacial succession gradients in Antarctica; and AIM 3) Measure the relative effects of fundamental plant functional traits on soil microbial and invertebrate communities across glacial succession gradients in Antarctica. They will explore how early-colonizing plants, especially mosses and lichens, alter soil physical, biogeochemical, and biological components, potentially impacting later patterns of succession. The researhcers will use intensive surveys of plant-soil interactions across succession sites and a manipulative transplant experiment in the South Shetland Islands, Antarctica to address their aims. The investigators will collect data on plant functional traits and their effects on soil physical properties, biogeochemistry, biotic abundance, and microbial metagenomics. The data collected will be the first comprehensive measures of the relative importance of plant functional types during glacial retreat and vegetative expansion from climate change in Antarctica, aiding our understanding of how plant functional group diversity and abundance are changing in a greening Antarctica. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
This project seeks to make detailed measurements of the oxygen content of the surface ocean along the Western Antarctic Peninsula. Detailed maps of changes in net oxygen content will be combined with measurements of the surface water chemistry and phytoplankton distributions. The project will determine the extent to which on-shore or offshore phytoplankton blooms along the peninsula are likely to lead to different amounts of carbon being exported to the deeper ocean. The project team members will participate in the development of new learning tools at the Museum of Life and Science. They will also teach secondary school students about aquatic biogeochemistry and climate, drawing directly from the active science supported by this grant. The project will analyze oxygen in relation to argon that will allow determination of the physical and biological contributions to surface ocean oxygen dynamics. These assessments will be combined with spatial and temporal distributions of nutrients (iron and macronutrients) and irradiances. This will allow the investigators to unravel the complex interplay between ice dynamics, iron and physical mixing dynamics as they relate to Net Community Production (NCP) in the region. NCP measurements will be normalized to Particulate Organic Carbon (POC) and be used to help identify area of "High Biomass and Low NCP" and those with "Low Biomass and High NCP" as a function of microbial plankton community composition. The team will use machine learning methods- including decision tree assemblages and genetic programming- to identify plankton groups key to facilitating biological carbon fluxes. Decomposing the oxygen signal along the West Antarctic Peninsula will also help elucidate biotic and abiotic drivers of the O2 saturation to further contextualize the growing inventory of oxygen measurements (e.g. by Argo floats) throughout the global oceans.
The Antarctic ice sheet is underlain by a dynamic water system that lubricates the flow of ice streams and outlet glaciers, provides a habitat for a diverse microbial ecosystem, and delivers freshwater and nutrients to the Southern Ocean. However, imaging this subglacial environment is difficult: Antarctica is a vast continent with ice up to four kilometers (2.5 miles) thick. To detect water at the ice-bed interface and in deeper groundwater reservoirs, this project will adapt a technique called electromagnetic sounding that is well-established on land and in the ocean for imaging fluids beneath the surface. Groundwater is estimated to be a significant part of the subglacial water budget in Antarctica, yet previous observational approaches have been unable to characterize its volume and distribution. This project will thus yield critical information about how ice-rock-water-ocean systems interact and inform our understanding of ice-sheet processes, global nutrient cycles, and freshwater flux to the ocean. The project will provide cross-disciplinary training for a graduate student and postdoctoral scientist, and develop an educational outreach program through the Birch Aquarium. Standard geophysical surveying techniques used in glaciology to image subglacial water (radio-echo sounding and active-source seismology) are not directly sensitive to water content. In contrast, ground-based electromagnetic (EM) methods are sensitive to water content through its impact on bulk conductivity. Although EM methods are well-established for high-precision mapping of hydrology in other geological environments, their application on ice sheets is in its infancy. The proposed work will adapt both passive- and active-source EM techniques to glaciological questions to quantify the three-dimensional structure of subglacial water beneath an ice stream and in a grounding zone. The project will perform a suite of synthetic inversion studies to determine the range of applications of EM techniques in glaciology and execute a field experiment on the Whillans Ice Plain to investigate two hypotheses about the subglacial water system based on previous observational and modeling results: (1) Subglacial Lake Whillans is underlain by a deep, saline groundwater reservoir; and (2) there is an estuary-like zone of mixing between fresh subglacial water and seawater near, and possibly landward, of the grounding line.
Antarctic groundwater drives the regional carbon cycle and can accelerate permafrost thaw shaping Antarctic surface features. However, groundwater extent, flow, and processes on a continent virtually locked in ice are poorly understood. The proposed work investigates the interplay between groundwater, sediment, and ice in Antarctica's cold desert landscape to determine when, where, and why Antarctic groundwater is flowing, and how it may evolve Antarctic frozen deserts from dry and stable to wet and dynamic. Mapping the changing extent of Antarctic near-surface groundwater requires the ability to measure soil moisture rapidly and repeatedly over large areas. The research will capture changes in near-surface groundwater distribution through an unmanned aerial vehicle (UAV) mapping approach. The project integrates a diverse range of sensors with new UAV technologies to provide a higher-resolution and more frequent assessment of Antarctic groundwater extent and composition than can be accomplished using satellite observations alone. To complement the research objectives, the PI will develop a new UAV summer field school, the Geosciences UAV Academy, focused on training undergraduate-level UAV pilots in conducting novel earth sciences research using cutting edge imaging tools. The integration of research and technology will prepare students for careers in UAV-related industries and research. The project will deliver new UAV tools and workflows for soil moisture mapping relevant to arid regions including Antarctica as well as temperate desert and dryland systems and will train student research pilots to tackle next generation airborne challenges. Water tracks are the basic hydrological unit that currently feeds the rapidly-changing permafrost and wetlands in the Antarctic McMurdo Dry Valleys (MDV). Despite the importance of water tracks in the MDV hydrologic cycle and their influence on biogeochemistry, little is known about how these water tracks control the unique brine processes operating in Antarctic ice-free areas. Both groundwater availability and geochemistry shape Antarctic microbial communities, connecting soil geology and hydrology to carbon cycling and ecosystem functioning. The objectives of this CAREER proposal are to 1) map water tracks to determine the spatial distribution and seasonal magnitude of groundwater impacts on the MDV near-surface environment to determine how near-surface groundwater drives permafrost thaw and enhances chemical weathering and biogeochemical cycling; 2) establish a UAV academy training earth sciences students to answer geoscience questions using drone-based platforms and remote sensing techniques; and 3) provide a formative step in the development of the PI as a teacher-scholar. UAV-borne hyperspectral imaging complemented with field soil sampling will determine the aerial extent and timing of inundation, water level, and water budget of representative water tracks in the MDV. Soil moisture will be measured via near-infrared reflectance spectroscopy while bulk chemistry of soils and groundwater will be analyzed via ion chromatography and soil x-ray fluorescence. Sedimentological and hydrological properties will be determined via analysis of intact core samples. These data will be used to test competing hypotheses regarding the origin of water track solutions and water movement through seasonal wetlands. The work will provide a regional understanding of groundwater sources, shallow groundwater flux, and the influence of regional hydrogeology on solute export to the Southern Ocean and on soil/atmosphere linkages in earth's carbon budget. The UAV school will 1) provide comprehensive instruction at the undergraduate level in both how and why UAVs can advance geoscience research and learning; and 2) provide educational infrastructure for an eventual self-sustaining summer program for undergraduate UAV education. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
Microbial mats are found throughout the McMurdo Dry Valleys where summer snowmelt provides liquid water that allows these mats to flourish. Researchers have long studied the environmental conditions microbial mats need to grow. Despite these efforts, it has been difficult to develop a broad picture of these unique ecosystems. Recent advances in satellite technology now provide researchers an exciting new tool to study these special Antarctic ecosystems from space using the unique spectral signatures associated with microbial mats. This new technology not only offers the promise that microbial mats can be mapped and studied from space, this research will also help protect these delicate environments from potentially harmful human impacts that can occur when studying them from the ground. This project will use satellite imagery and spectroscopic techniques to identify and map microbial mat communities and relate their properties and distributions to both field and lab-based measurements. This research provides an exciting new tool to help document and understand the distribution of a major component of the Antarctic ecosystem in the McMurdo Dry Valleys. The goal of this project is to establish quantitative relationships between spectral signatures derived from orbit and the physiological status and biogeochemical properties of microbial mat communities in Taylor Valley, Antarctica, as measured by field and laboratory analyses on collected samples. The goal wioll be met by (1) refining atmospheric correction techniques using in situ radiometric rectification to derive accurate surface spectra; (2) collecting multispectral orbital images concurrent with in situ sampling and spectral measurements in the field to ensure temporal comparability; (3) measuring sediment, water, and microbial mat samples for organic and inorganic carbon content, essential biogeochemical nutrients, and chlorophyll-a to determine relevant mat characteristics; and (4) quantitatively associating these laboratory-derived characteristics with field-derived and orbital spectral signatures and parameters. The result of this work will be a more robust quantitative link between the distribution of microbial mat communities and their biogeochemical properties to landscape-scale spectral signatures. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
Part I: Non-technical description: Earth’s terrestrial ecosystems have the potential to either slow down or hasten the pace of climate change. The direction depends in part on both plant and microbial responses to warming. This study uses Antarctica as a model ecosystem to study the carbon balance of a simplified ecosystem (simplified compared to terrestrial ecosystems elsewhere) in response to a warming treatment. Carbon balance is dictated by sequestered carbon (through photosynthesis) and released carbon (plant and microbial respiration). Hence, to best assess plant and microbial responses to warming, this study uses a plant gradient that starts at the glacier (no plants, only soil microbes) to an old site entirely covered by plants. Experimental warming in the field is achieved by open-top chambers that warm the air and soil inside. The net ecosystem carbon exchange, the net result of sequestered and released carbon, will be measured in warmed and control plots with a state-of-the art gas exchange machine. Laboratory temperature incubation studies will supplement field work to attribute changes in carbon fluxes to individual plant species and soil microbial taxa (i.e., “species”). Data from this study will feed into earth system climate change models. The importance of this study will be shared with the broader community through the production of a video series created by an award-winning science media production company, an Antarctic blog, and through interactions with schools in the United States (on-site through Skype and in-person visits). Part II: Technical description: Responses of the carbon balance of terrestrial ecosystems to warming will feed back to the pace of climate change, but the size and direction of this feedback are poorly constrained. Least known are the effects of warming on carbon losses from soil, and clarifying the major microbial controls is an important research frontier. This study uses a series of experiments and observations to investigate microbial, including autotrophic taxa, and plant controls of net ecosystem productivity in response to warming in intact ecosystems. Field warming is achieved using open-top chambers paired with control plots, arrayed along a productivity gradient. Along this gradient, incoming and outgoing carbon fluxes will be measured at the ecosystem-level. The goal is to tie warming-induced shifts in net ecosystem carbon balance to warming effects on soil microbes and plants. The field study will be supplemented with lab temperature incubations. Because soil microbes dominate biogeochemical cycles in Antarctica, a major focus of this study is to determine warming responses of bacteria, fungi and archaea. This is achieved using a cutting-edge stable isotope technique, quantitative stable isotope probing (qSIP) developed by the proposing research team, that can identify the taxa that are active and involved in processing new carbon. This technique can identify individual microbial taxa that are actively participating in biogeochemical cycling of nutrients (through combined use of 18O-water and 13C-bicarbonate) and thus can be distinguished from those that are simply present (cold-preserved). The study further assesses photosynthetic uptake of carbon by the vegetation and their sensitivity to warming. Results will advance research in climate change, plant and soil microbial ecology, and ecosystem modeling. Science communication will be achieved through an informative video series, a daily Antarctic blog, and online- and in-person visits to schools in the United States. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
Part I: Non-technical description: Methane is one of the more effective atmospheric gases at retaining heat in the lower atmosphere and the earth’s crust contains large quantities of methane. Research that identifies the factors that control methane’s release into the atmosphere is critical to understanding and mitigating climate change. One of the most effective natural processes that inhibits the release of methane from aquatic habitats is a community of bacteria and Archaea (microbes) that use the chemical energy stored in methane, transforming methane into less-climate-sensitive compounds. The amount of methane that may be released in Antarctica is unknown, and it is unclear which microbes consume the methane before it is released from the ocean in Antarctica. This project will study one of the few methane seeps known in Antarctica to advance our understanding of which microbes inhibit the release of methane in marine environments. The research will also identify if methane is a source of energy for other Antarctic organisms. The researchers will analyze the microbial species associated with methane consumption over several years of field and laboratory research based at an Antarctic US station, McMurdo. This project clearly expands the fundamental knowledge of Antarctic systems, biota, and processes outlined as a goal in the Antarctic solicitation. This research communicates and produces educational material for K-12, college, and graduate students to inspire and inform the public about the role Antarctic ecosystems play in the global environment. This project also provides a young professor an opportunity to establish himself as an expert in the field of Antarctic microbial ecology to help solidify his academic career. Part II: Technical description: Microbes act as filter to methane release from the ocean into the atmosphere, where microbial chemosynthetic production harvests the chemical energy stored in this greenhouse gas. In spite of methane reservoirs in Antarctica being as large as Arctic permafrost, we know only a little about the taxa or dominant processes involved in methane consumption in Antarctica. The principal investigator will undertake a genomic and transcriptomic study of microbial communities developed and still developing after initiation of methane seepage in McMurdo Sound. An Antarctic methane seep was discovered at this location in 2012 after it began seeping in 2011. Five years after it began releasing methane, the methane-oxidizing microbial community was underdeveloped and methane was still escaping from the seafloor. This project will be essential in elucidating the response of microbial communities to methane release and identify how methane oxidation occurs within the constraints of the low polar temperatures. This investigation is based on 4 years of field sampling and will establish a time series of the development of cold seep microbial communities in Antarctica. A genome-to-ecosystem approach will establish how the Southern Ocean microbial community is adapted to prevent methane release into the ocean. As methane is an organic carbon source, results from this study will have implications for the Southern Ocean carbon cycle. Two graduate students will be trained and supported with undergraduates participating in laboratory activities. The researcher aims to educate, inspire and communicate about Antarctic methane seeps to a broad community. A mixed-media approach, with videos, art and education in schools will be supported in collaboration with a filmmaker, teachers and a visual artist. Students will be trained in filmmaking and K-12 students from under-represented communities will be introduced to Antarctic science through visual arts. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
Viruses are prevalent in aquatic environments where they reach up to five hundred million virus particles in a teaspoon of water. Ongoing discovery of viruses seems to confirm current understanding that all forms of life can host and be infected by viruses and that viruses are one of the largest reservoirs of unexplored genetic diversity on Earth. This study aims to better understand interactions between specific viruses and phytoplankton hosts and determine how these viruses may affect different algal groups present within lakes of the Vestfold Hills, Antarctica. These lakes (Ace, Organic and Deep)were originally derived from the ocean and contain a broad range of saline conditions with a similarly broad range of physicochemical characteristics resulting from isolation and low external influence for thousands of years. These natural laboratories allow examination of microbial processes and interactions that would be difficult to characterize elsewhere on earth. The project will generate extensive genomic information that will be made freely available. The project will also leverage the study of viruses and the genomic approaches employed to advance the training of undergraduate students and to engage and foster an understanding of Antarctic science and studies of microbes during a structured informal education program in Maine for the benefit of high school students. By establishing the dynamics and interactions of (primarily) specific dsDNA virus groups in different habitats with different redox conditions throughout seasonal and inter annual cycles the project will learn about the biotic and abiotic factors that influence microbial community dynamics. This project does not require fieldwork in Antarctica. Instead, the investigators will leverage already collected and archived samples from three lakes that have concurrent measures of physicochemical information. Approximately 2 terabyte of Next Generation Sequencing (NGS) (including metagenomes, SSU rRNA amplicons and single virus genomes) will be generated from selected available samples through a Community Science Program (CSP) funded by the Joint Genome Institute. The investigators will employ bioinformatics to interrogate those sequence databases. In particular, they will focus on investigating the presence, phylogeny and co-occurrence of polintons, polinton-like viruses, virophages and large dsDNA phytoplankton viruses as well as of their putative eukaryotic microbial hosts. Bioinformatic analyses will be complemented with quantitative digital PCR and microbial association network analysis to detect specific virus?virus?host interactions from co-occurrence spatial and temporal patterns. Multivariate analysis and network analyses will also be performed to investigate which abiotic factors most closely correlate with phytoplankton and virus abundances, temporal dynamics, and observed virus-phytoplankton associations within the three lakes. The results of this project will improve understanding of phytoplankton and their viruses as vital components of the carbon cycle in Antarctic, marine-derived aquatic environments, and likely in any other aquatic environment. Overall, this work will advance understanding of the genetic underpinnings of adaptations in unique Antarctic environments.
Western Antarctica is one of the fastest warming locations on Earth. Its changing climate will lead to an increase in sea-level and will also alter regional water temperature and chemistry. These changes will directly alter the microbes that inhabit the ecosystem. Microbes are the smallest forms of life on Earth, but they are also the most abundant. They drive cycling of essential nutrients, such as carbon and nitrogen that are found in ocean sediments. In this way they form the foundation of the food chain that supports larger and more complex life. However, we do not know much about how different communities of microbes break down sediments in Antarctica and this will influence the chemistry of those waters. This research will determine how communities of microbes on the coastal shelf of Antarctica degrade complex organic sediments using genetic and chemical data. This data will identify the species in the community, what enzymes they are producing and what chemical reactions they are driving. This research will create broader impacts as the data will be used to create in-class activities that improve a student’s data analysis and critical thinking skills. The data will be used in graduate, undergraduate and K-12 classrooms. This research will provide genetic and enzymatic insight into how microbial communities in benthic sediments on the coastal shelf of Antarctica degrade complex organic matter. The current understanding of how benthic microbial communities respond to and then degrade complex organic matter in Antarctica is fragmented. Recent work suggests benthic microbial communities are shaped by organic matter availability. However, those studies were observational and did not directly examine community function. A preliminary study of metagenomic data from western Antarctic marine sediments, indicates a genetic potential for organic matter degradation but functional data was not been collected. Other studies have examined either enzyme activity or metagenomic potential, but few have been able to directly connect the two. To address this gap in knowledge, this study will utilize metagenomics and metatranscriptomics, coupled with microcosm experiments, enzyme assays, and geochemical data. It will examine Antarctic microbial communities from the Ross Sea, the Bransfield Strait and Weddell Sea to document how the relationship between a communities’ enzymatic activity and the genes used to degrade complex organic matter is related to sediment breakdown. The data will expand our current knowledge of microbial genetic potential and provide a solid understanding of enzyme function as it relates to degradation of complex organic matter in those marine sediments. It will thereby improve our understanding of temperature change on the chemistry of Antarctic seawater. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
Antarctica’s native animals face increasing stressors from warming oceans. A key unanswered question is how Antarctic life will respond. If warmer waters contribute to fish disease susceptibility, then iconic Antarctic predators they support, including penguins, seals, and killer whales, will suffer. A recent scientific cruise on the Antarctic peninsula encountered a population of crowned notothen fish that were plagued by pink, wart-like tumors that covered 10% to 30% of the body surface on about a third of the animals. Similar tumors had not previously been reported, suggesting that this might be a new disease that threatens Antarctic fish. The goal of proposed work is to identify the biological origins of the tumor and how it affects cell function and organismal physiology. The work is potentially transformative because it studies what might be a harbinger of Antarctic fish responses to global climate change. The project has several Broader Impacts. First, it will publicize the tumors. Because Antarctic researchers have never reported a tumor epidemic, the community must become aware of the outbreak and the tumor’s distinct diagnostic features. Second, dissemination of project results will stir further research to determine if this is an isolated event or is becoming a general phenomenon, and thus a broad concern for Antarctic ecosystems. Third, assays the project develops to detect the disease will enhance research infrastructure. Finally, work will broaden the nation’s scientific workforce by providing authentic research experiences for high school students and undergraduates from groups underrepresented in scientific research. The overall goal of proposed work is to identify the biological origins of the neoplasia and how it affects cell function and physiology. Aim 1 is to identify the pathogenic agent. Aim 1a is to test the hypothesis that a virus causes the neoplasia by isolating and sequencing viral nucleic acids from neoplasias and from animals that are not visibly affected. Aim 1b is to test neoplasias for bacteria, fungi, protozoa, or invertebrate parasites not present in healthy skin. Aim 2 is to learn how the disease alters the biology of affected cells. Aim 2a is to examine histological sections of affected and control tissues to see if the neoplasias are similar to previously reported skin diseases in temperate water fishes. Aim 2b is to examine the function of neoplastic cells by RNA-seq transcriptomics to identify genes that are differentially expressed in neoplasias and normal skin. Achieving these Aims will advance knowledge by identifying the causes and consequences of an outbreak of neoplasias in Antarctic fish. Proposed work is significant because it is the first to investigate a neoplasia cluster in Antarctic fish. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
Part I: Non-technical summary: This project focuses on understanding annual changes in microbial life that grows on the bottom of Lake Fryxell, Antarctica. Because of its polar latitude, photosynthesis can only occur during the summer months. During summer, photosynthetic bacteria supply communities with energy and oxygen. However, it is unknown how the microbes behave in the dark winter, when observations are not possible. This project will install environmental monitors and light-blocking shades over parts of these communities. The shades will extend winter conditions into the spring to allow researchers to characterize the winter behavior of the microbial communities. Researchers will measure changes in the water chemistry due to microbial activities when the shades are removed and the mats first receive light. Results are expected to provide insights into how organisms interact with and change their environments. The project includes training of graduate students and early career scientists in fieldwork, including scientific ice diving techniques. In addition, the members of the project team will develop a web-based “Guide to Thrive”, which will compile field tips ranging from basic gear use to advanced environmental protection techniques. This will be a valuable resource for group leaders ranging from undergraduate teaching assistants to Antarctic expedition leaders to lead well-planned and tailored field expeditions. Part II: Technical summary: The research team will measure seasonal metabolic and biogeochemical changes in benthic mats using differential gene expression and geochemical gradients. They will identify seasonal phenotypic differences in microbial communities and ecosystem effects induced by spring oxygen production. To do so, researchers will install environmental sensors and opaque shades over mats at three depths in the lake. The following spring, shaded and unshaded mats will be sampled. The shades will then be removed, and changes in pore water O2, H2S, pH, and redox will be measured using microelectrodes. Mats will also be sampled for transcriptomic gene expression analyses at intervals guided by geochemical changes. Pore water will be sampled for nutrient analyses. Field research will be supplemented with laboratory experiments to refine field techniques, gene expression data analysis, and integration of results into a seasonal model of productivity and nitrogen cycling in Lake Fryxell. Results will provide insights into several key priorities for NSF, including how biotic, abiotic and environmental components of the benthic mats interact to affect Antarctic lakes. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
The coastal Antarctic is undergoing great environmental change. Physical changes in the environment, such as altered sea ice duration and extent, have a direct impact on the phytoplankton and bacteria species which form the base of the marine foodweb. Photosynthetic phytoplankton are the ocean's primary producers, transforming (fixing) CO2 into organic carbon molecules and providing a source of food for zooplankton and larger predators. When phytoplankton are consumed by zooplankton, or killed by viral attack, they release large amounts of organic carbon and nutrients into the environment. Heterotrophic bacteria must eat other things, and function as "master recyclers", consuming these materials and converting them to bacterial biomass which can feed larger organisms such as protists. Some protists are heterotrophs, but others are mixotrophs, able to grow by photosynthesis or heterotrophy. Previous work suggests that by killing and eating bacteria, protists and viruses may regulate bacterial populations, but how these processes are regulated in Antarctic waters is poorly understood. This project will use experiments to determine the rate at which Antarctic protists consume bacteria, and field studies to identify the major bacterial taxa involved in carbon uptake and recycling. In addition, this project will use new sequencing technology to obtain completed genomes for many Antarctic marine bacteria. To place this work in an ecosystem context this project will use microbial diversity data to inform rates associated with key microbial processes within the PALMER ecosystem model. This project addresses critical unknowns regarding the ecological role of heterotrophic marine bacteria in the coastal Antarctic and the top-down controls on bacterial populations. Previous work suggests that at certain times of the year grazing by heterotrophic and mixotrophic protists may meet or exceed bacterial production rates. Similarly, in more temperate waters bacteriophages (viruses) are thought to contribute significantly to bacterial mortality during the spring and summer. These different top-down controls have implications for carbon flow through the marine foodweb, because protists are grazed more efficiently by higher trophic levels than are bacteria. This project will use a combination of grazing experiments and field observations to assess the temporal dynamics of mortality due to temperate bacteriophage and protists. Although many heterotrophic bacterial strains observed in the coastal Antarctic are taxonomically similar to strains from other regions, recent work suggest that they are phylogenetically and genetically distinct. To better understand the ecological function and evolutionary trajectories of key Antarctic marine bacteria, their genomes will be isolated and sequenced. Then, these genomes will be used to improve the predictions of the paprica metabolic inference pipeline, and our understanding of the relationship between heterotrophic bacteria and their major predators in the Antarctic marine environment. Finally, researchers will modify the Regional Test-Bed Model model to enable microbial diversity data to be used to optimize the starting conditions of key parameters, and to constrain the model's data assimilation methods. There is an extensive education and outreach component to this project that is designed to engage students and the public in diverse activities centered on Antarctic microbiota and marine sciences. A new module on Antarctic marine science will be developed for the popular Sally Ride Science program, and two existing undergraduate courses at UC San Diego will be strengthened with laboratory modules introducing emerging technology, and with cutting-edge polar science. A PhD student and a post-doctoral researcher will be supported by this project. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
Part 1: Nitrification is the conversion of ammonium to nitrate by a two-step process involving two different guilds of microorganisms: ammonia- and nitrite-oxidizers. The process is central to the global nitrogen cycle, affecting everything from retention of fertilizer on croplands to removal of excess nitrogen from coastal waters before it can cause blooms of harmful algae. It also produces nitrous oxide, an ozone-destroying, greenhouse gas. The energy derived from both steps of nitrification is used to convert inorganic carbon into microbial biomass. The biomass produced contributes to the overall food web production of the Southern Ocean and may be a particularly important subsidy during winter when low light levels restrict the other major source of biomass, primary production by single-celled plants. This project addresses three fundamental questions about the biology and geochemistry of polar oceans, with a focus on the process of nitrification. The first question the project will address concerns the contribution of chemoautotrophy (based on nitrification) to the overall supply of organic carbon to the food web of the Southern Ocean. Previous measurements indicate that it contributes about 9% to the Antarctic food web on an annual basis, but those measurements did not include the additional production associated with nitrite oxidation. The second question to be addressed is related to the first and concerns the coupling between the steps of the process. The third seeks to determine the significance of the contribution of other sources of nitrogen, (specifically organic nitrogen and urea released by other organisms) to nitrification because these contributions may not be assessed by standard protocols. Measurements made by others suggest that urea in particular might be as important as ammonium to nitrification in polar regions. This project will result in training a postdoctoral researcher and provide undergraduate students opportunities to gain hand-on experience with research on microbial geochemistry. The Palmer LTER (PAL) activities have focused largely on the interaction between ocean climate and the marine food web affecting top predators. Relatively little effort has been devoted to studying processes related to the microbial geochemistry of nitrogen cycling as part of the Palmer Long Term Ecological Research (LTER) program, yet these are a major themes at other sites. This work will contribute substantially to understanding an important aspect of nitrogen cycling and bacterioplankton production in the PAL-LTER study area. The team will be working synergistically and be participating fully in the education and outreach efforts of the Palmer LTER, including making highlights of the findings available for posting to their project web site and participating in any special efforts they have in the area of outreach. Part 2: The proposed work will quantify oxidation rates of 15N supplied as ammonium, urea and nitrite, allowing us to estimate the contribution of urea-derived N and complete nitrification (ammonia to nitrate) to chemoautotrophy and bacterioplankton production in Antarctic coastal waters. The project will compare these estimates to direct measurements of the incorporation of 14C into organic matter the dark for an independent estimate of chemoautotrophy. The team aims to collect samples spanning the water column: from surface water (~10 m), winter water (50-100 m) and circumpolar deep water (>150 m); on a cruise surveying the continental shelf and slope west of the Antarctic Peninsula in the austral summer of 2018. Other samples will be taken to measure the concentrations of nitrate, nitrite, ammonia and urea, for qPCR analysis of the abundance of relevant microorganisms, and for studies of related processes. The project will rely on collaboration with the existing Palmer LTER to ensure that ancillary data (bacterioplankton abundance and production, chlorophyll, physical and chemical variables) will be available. The synergistic activities of this project along with the LTER activities will provide a unique opportunity to assess chemoautotrophy in context of the overall ecosystem?s dynamics- including both primary and secondary production processes.
The project will characterize the functional, taxonomic, biotic and abiotic drivers of soil ecosystems in the Trans Antarctic Mountains (one of the most remote and harsh terrestrial landscapes on the planet). The work will utilize new high-throughput DNA and RNA sequencing technologies to identify members of the microbial communities and determine if the microbial community structures are independent of local environmental heterogeneities. In addition the project will determine if microbial diversity and function are correlated with time since the last glacial maximum (LGM). The expected results will greatly contribute to our knowledge regarding rates of microbial succession and help define the some of the limits to life and life-maintaining processes on Earth. The project will analyze genomes and RNA derived from these genomes to describe the relationships between biodiversity and ecosystem functioning from soils above and below LGM elevations and to correlate these with the environmental drivers associated with their development during the last ~18,000 years. The team will identify the taxonomic diversity and the functional genetic composition within a broad suite of soil biota and examine their patterns of assembly and distribution within the framework of their geological legacies. The project will mentor participants from undergraduate students to postdoctoral researchers and prepare them to effectively engage in research to meet their career aspirations. The project will contribute to ongoing public education efforts through relationships with K-12 teachers and administrators- to include University-Public School partnerships. Less formal activities include public lecture series and weblogs aimed at providing information on Antarctic polar desert ecosystems to the general public. Targeted classrooms near each PI's institution will participate in online, real-time discussions about current topics in Antarctic ecosystems research.
A profound transformation in ecosystem structure and function is occurring in coastal waters of the western Weddell Sea, with the collapse of the Larsen B ice shelf. This transformation appears to be yielding a redistribution of energy flow between chemoautotrophic and photosynthetic production, and to be causing the rapid demise of the extraordinary seep ecosystem discovered beneath the ice shelf. This event provides an ideal opportunity to examine fundamental aspects of ecosystem transition associated with climate change. We propose to test the following hypotheses to elucidate the transformations occurring in marine ecosystems as a consequence of the Larsen B collapse: (1) The biogeographic isolation and sub-ice shelf setting of the Larsen B seep has led to novel habitat characteristics, chemoautotrophically dependent taxa and functional adaptations. (2) Benthic communities beneath the former Larsen B ice shelf are fundamentally different from assemblages at similar depths in the Weddell sea-ice zone, and resemble oligotrophic deep-sea communities. Larsen B assemblages are undergoing rapid change. (3) The previously dark, oligotrophic waters of the Larsen B embayment now support a thriving phototrophic community, with production rates and phytoplankton composition similar to other productive areas of the Weddell Sea. To document rapid changes occurring in the Larsen B ecosystem, we will use a remotely operated vehicle, shipboard samplers, and moored sediment traps. We will characterize microbial, macrofaunal and megafaunal components of the seep community; evaluate patterns of surface productivity, export flux, and benthic faunal composition in areas previously covered by the ice shelf, and compare these areas to the open sea-ice zone. These changes will be placed within the geological, glaciological and climatological context that led to ice-shelf retreat, through companion research projects funded in concert with this effort. Together these projects will help predict the likely consequences of ice-shelf collapse to marine ecosystems in other regions of Antarctica vulnerable to climate change. The research features international collaborators from Argentina, Belgium, Canada, Germany, Spain and the United Kingdom. The broader impacts include participation of a science writer; broadcast of science segments by members of the Jim Lehrer News Hour (Public Broadcasting System); material for summer courses in environmental change; mentoring of graduate students and postdoctoral fellows; and showcasing scientific activities and findings to students and public through podcasts.
In Antarctica, millions of years of freezing have led to the development of hundreds of meters of thick permafrost (i.e., frozen ground). Recent research demonstrated that this slow freezing has trapped and concentrated water into local and regional briny aquifers, many times more salty than seawater. Because salt depresses the freezing point of water, these saline brines are able to persist as liquid water at temperatures well below the normal freezing point of freshwater. Such unusual groundwater systems may support microbial life, supply nutrients to coastal ocean and ice-covered lakes, and influence motion of glaciers. These briny aquifers also represent potential terrestrial analogs for deep life habitats on other planets, such as Mars, and provide a testing ground for the search for extraterrestrial water. Whereas much effort has been invested in understanding the physics, chemistry, and biology of surface and near-surface waters in cold polar regions, it has been comparably difficult to investigate deep subsurface aquifers in such settings. Airborne ElectroMagnetics (AEM) subsurface imaging provides an efficient way for mapping salty groundwater. An international collaboration with the University of Aarhus in Denmark will enable knowledge and skill transfer in AEM techniques that will enhance US polar research capabilities and provide US undergraduates and graduate students with unique training experiences. This project will survey over 1000 km2 of ocean and land near McMurdo Station in Antarctica, and will reveal if cold polar deserts hide a subsurface pool of liquid water. This will have significant implications for understanding cold polar glaciers, ice-covered lakes, frozen ground, and polar microbiology as well as for predictions of their response to future change. Improvements in permafrost mapping techniques and understanding of permafrost and of underlying groundwaters will benefit human use of high polar regions in the Antarctic and the Arctic. The project will provide the first integrative system-scale overview of subsurface water distribution and hydrological connectivity in a partly ice-free coastal region of Antarctica, the McMurdo Dry Valleys. Liquid water is relatively scarce in this environment but plays an outsized role by influencing, and integrating, biological, biogeochemical, glaciological, and geological processes. Whereas surface hydrology and its role in ecosystem processes has been thoroughly studied over the last several decades, it has been difficult to map out and characterize subsurface water reservoirs and to understand their interactions with regional lakes, glaciers, and coastal waters. The proposed project builds on the "proof-of-concept" use of AEM technology in 2011. Improvements in sensor and data processing capabilities will result in about double the depth of penetration of the subsurface during the new data collection when compared to the 2011 proof-of-concept survey, which reached depths of 300-400m. The first field season will focus on collecting deep soundings with a ground-based system in key locations where: (i) independent constraints on subsurface structure exist from past drilling projects, and (ii) the 2011 resistivity dataset indicates the need for deeper penetration and high signal-to-noise ratios achievable only with a ground-based system. The regional airborne survey will take place during the second field season and will yield subsurface electrical resistivity data from across several valleys of different sizes and different ice cover fractions.
Despite recent advances, we still know little about how life and its traces persist in extremely harsh conditions. What survival strategies do cells employ when pushed to their limit? Using a new technique, this project will investigate whether Antarctic paleolakes harbor "microbial seed banks," or caches of viable microbes adapted to past paleoenvironments that could help transform our understanding of how cells survive over ancient timescales. Findings from this investigation could also illuminate novel DNA repair pathways with possible biomedical and biotechnology applications and help to refine life detection strategies for Mars. The project will bring Antarctic research to Georgetown University's campus for the first time, providing training opportunities in cutting edge analytical techniques for multiple students and a postdoctoral fellow. The field site will be the McMurdo Dry Valleys, which provide an unrivaled opportunity to investigate fundamental questions about the persistence of microbial life. Multiple lines of evidence, from interbedded and overlying ashfall deposits to parameterized models, suggest that the large-scale landforms there have remained essentially fixed as far back as the middle of the Miocene Epoch (i.e., ~8 million years ago). This geologic stability, coupled with geographic isolation and a steady polar climate, mean that biological activity has probably undergone few qualitative changes over the last one to two million years. The team will sample paleolake facies using sterile techniques from multiple Dry Valleys sites and extract DNA from entombed organic material. Genetic material will then be sequenced using Pacific Biosciences' Single Molecule, Real-Time DNA sequencing technology, which sequences native DNA as opposed to amplified DNA, thereby eliminating PCR primer bias, and enables read lengths that have never before been possible. The data will be analyzed with a range of bioinformatic techniques, with results that stand to impact our understanding of cell biology, Antarctic paleobiology, microbiology and biogeography, biotechnology, and planetary science.
The Southern Ocean in the vicinity of Antarctica is a region characterized by seasonally-driven marine phytoplankton blooms that are often dominated by microalgal species which produce large amounts of dimethylsulfoniopropionate (DMSP). DMSP can be converted to the compound dimethylsulfide (DMS) which is a molecule that can escape into the atmosphere where it is known to have strong condensation properties that are involved in regional cloud formation. Production of DMSP can influence the diversity and composition of microbial assemblages in seawater and the types and activities of microbes in the seawater will likely affect the magnitude of DMSP\DMS production. The proposal aims to examine the role of DMSP in structuring the microbial communities in Antarctic waters and how this structuring may influence DMSP cycling. The project will leverage the Antarctic research to introduce concepts and data linking microbial diversity and biogeochemistry to a range of audiences (including high school and undergraduate students in Maine). The project will also engage teacher and students in rural K-8 schools and will allow a collaboration with a science writer and illustrator who will join the team in the field. The writer will use the southern ocean experience as the setting for a poster and a book about the proposed research and the scientists studying extreme environments. The project will examine (1) the extent to which the cycling of DMSP in southern ocean waters influences the composition and diversity of bacterial and protistan assemblages; (2) conversely, whether the composition and diversity of southern ocean protistan and bacterial assemblages influence the magnitude and rates of DMSP cycling; (3) the expression of DMSP degradation genes by marine bacteria seasonally and in response to additions of DMSP; and, to synthesize these results by quantifying (4) the microbial networks resulting from the presence of DMSP-producers and DMSP-consumers along with their predators, all involved in the cycling of DMSP in southern ocean waters. The work will be accomplished by conducting continuous growth experiments with DMSP-amended natural samples during field sampling of different microbial communities present in summer and fall. Data from the molecular (such as 16S/ 18S tag sequences, DMSP-cycle gene transcripts) and biogeochemical (such as biogenic sulfur cycling, bacterial production, microbial biomass) investigations will be integrated via network analysis.
The Antarctic subglacial environment remains one of the least explored regions on Earth. This project will examine the physical and biological characteristics of Subglacial Lake Mercer, a lake that lies 1200m beneath the West Antarctic Ice Sheet. This study will address key questions relating to the stability of the ice sheet, the subglacial hydrological system, and the deep-cold subglacial biosphere. The education and outreach component aims to widely disseminate results to the scientific community and to the general public through short films, a blog, and a website. Subglacial Lake Mercer is one of the larger hydrologically active lakes in the southern basin of the Whillans Ice Plain, West Antarctica. It receives about 25 percent of its water from East Antarctica with the remainder originating from West Antarctica, is influenced by drain/fill cycles in a lake immediately upstream (Subglacial Lake Conway), and lies about 100 km upstream of the present grounding line of the Ross Ice Shelf. This site will yield information on the history of the Whillans and Mercer Ice Streams, and on grounding line migration. The integrated study will include direct sampling of basal ice, water, and sediment from the lake in concert with surface geophysical surveys over a three-year period to define the hydrological connectivity among lakes on the Whillans Ice Plain and their flow paths to the sea. The geophysical surveys will furnish information on subglacial hydrology, aid the site selection for hot-water drilling, and provide spatial context for interpreting findings. The hot-water-drilled boreholes will be used to collect basal ice samples, provide access for direct measurement of subglacial physical, chemical, and biological conditions in the water column and sediments, and to explore the subglacial water cavities using a remotely operated vehicle equipped with sensors, cameras, and sampling equipment. Data collected from this study will address the overarching hypothesis "Contemporary biodiversity and carbon cycling in hydrologically-active subglacial environments associated with the Mercer and Whillans ice streams are regulated by the mineralization and cycling of relict marine organic matter and through interactions among ice, rock, water, and sediments". The project will be undertaken by a collaborative team of scientists, with expertise in microbiology, biogeochemistry, hydrology, geophysics, glaciology, marine geology, paleoceanography, and science communication.
Atmospheric oxygen rose suddenly approximately 2.4 billion years ago after Cyanobacteria evolved the ability to produce oxygen through photosynthesis (oxygenic photosynthesis). This change permanently altered the future of life on Earth, yet little is known about the evolutionary processes leading to it. The Melainabacteria were first discovered in 2013 and are closely related non-photosynthetic relatives of the first group of organisms capable of oxygenic photosynthesis. This project will utilize existing data on metagenomes from microbial mats in Lake Vanda, an ice-covered lake in Antarctica where many sequences of Melainabacteria have been previously identified. From this genetic information, the project aims to assess the metabolic capabilities of these Melainabacteria and identify their potential ecological roles. The project will additionally evaluate the evolutionary relationships among the Cyanobacteria and Melainabacteria and closely related organisms that will allow an advancement in understanding of the evolutionary path that lead to oxygenic photosynthesis on Earth. The project will focus on extracting evolutionary information from the genomic data of Melainabacteria and Sericytochromatia, recently-described groups closely related to but basal to the Cyanobacteria. The characterization of novel members of these groups in samples from Lake Vanda, Antarctica, will provide insights into the path and processes involved in the evolution of oxygenic photosynthesis. The research will focus on assessing the metabolic capabilities of Melainabacteri, deriving the evolutionary relationships among Melainabacteria and Cyanobacteria and reconstructing potential evolutionary pathways leading to oxygenic photosynthesis. The project will focus on 12 metagenomes where the researchers expect to obtain genomes for at least the eight most abundant Melainabacteria in the dataset. Melainabacteria bins will be annotated and preliminary metabolic pathways will be constructed. The project will utilize full-length sequences of marker genes from across the bacterial domain with a particular focus on taxa that are oxygenic or anoxygenic phototrophs and use the marker genes, to build a rooted "backbone" tree. Incomplete or short sequences from the metagenomes will be added to the tree using the Evolutionary Placement Algorithm. The researchers will also build a corresponding phylogenetic tree using a Bayesian framework and compare their topologies. By doing so, the project aims to improve the understanding of the evolution of oxygenic photosynthesis, which caused the most significant change in Earth's surface chemistry. Specifically, they will document a significantly broader metabolic diversity within the Melainabacteria than has been previously identified, gain significant insights into their metabolic evolution, their evolutionary relationships with the Cyanobacteria, and the evolutionary steps leading to the origin of oxygenic photosynthesis. This research will have the overall effect of constraining key evolutionary processes in the origin of oxygenic photosynthesis. It will provide the foundation for future studies by indicating where a genomic record of the evolution of oxygenic photosynthesis may be preserved. Results will also be shared with middle school children through the development of scientific lesson plans in collaboration with teachers. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
Cryoconite holes are pockets of life completely encased in otherwise barren glacial ice. These pockets of life form when dust blown onto the ice melts a small, largely isolated hole that can function as its own tiny ecosystem. This dust can contain microorganisms such as bacteria, algae, or microscopic animals. The microorganisms within the hole interact and carry out functions typical of a larger ecosystem, such as a forest. Cryoconite holes are especially important in extreme cold environments such as the Antarctic Dry Valleys, where they function as repositories of life. Because cryoconite holes are mostly enclosed and persist for years, they can be tracked over time to test fundamental scientific questions about how communities of interacting organisms develop to become fully functioning ecosystems. This project will sample existing and experimentally created cryoconite holes to understand how these ecosystems develop and to what degree random processes (such as which organisms get there first) affect the final community composition and functioning. The results will not only improve our understanding of how microbial communities assemble and affect the functioning of microecosystems such as cryoconite holes, but also how the processes of community assembly affect functioning of larger ecosystems, such as forests. A better understanding of community establishment, development, and response to abiotic factors are essential to forecasting ecological responses to environmental change. It is essential to unravel the links between community assembly, biodiversity, and nutrient cycling across numerous ecosystems because these are critical factors determining ecological responses to environmental change. The unique, largely isolated nature of cryoconite holes provides an experimental system that will advance fundamental understanding of the processes (e.g., stochastic dynamics such as dispersal limitation, assembly order, and ecological drift) driving community assembly. This project will use a field sampling campaign and a number of manipulative experiments to test a hypothesis that unites theory in community and ecosystem ecology: the degree to which stochastic processes guide microbial community assembly and affects regional patterns in biodiversity and ecosystem processes. Cryoconite holes will be sampled to compare community composition, environmental factors, and ecosystem functioning between hydrologically connected and isolated holes. New cryoconite holes will also be constructed and monitored over the course of two growing seasons to specifically alter assembly order and community size, thereby pairing a unique manipulative experiment with field surveys to address questions with relevance to the Antarctic and beyond. Amplicon sequencing, metagenomics, microscopy, sensitive environmental chemistry methods, and photosynthesis and respiration measurements will be used to test a series of sub-hypotheses that relate stochasticity to patterns in regional biodiversity, heterogeneity in environmental factors, and ecosystem processes.
Phytoplankton blooms in the coastal waters of the Ross Sea, Antarctica are typically dominated by either diatoms or Phaeocystis Antarctica (a flagellated algae that often can form large colonies in a gelatinous matrix). The project seeks to determine if an association of bacterial populations with Phaeocystis antarctica colonies can directly supply Phaeocystis with Vitamin B12, which can be an important co-limiting micronutrient in the Ross Sea. The supply of an essential vitamin coupled with the ability to grow at lower iron concentrations may put Phaeocystis at a competitive advantage over diatoms. Because Phaeocystis cells can fix more carbon than diatoms and Phaeocystis are not grazed as efficiently as diatoms, the project will help in refining understanding of carbon dynamics in the region as well as the basis of the food web webs. Such understanding also has the potential to help refine predictive ecological models for the region. The project will conduct public outreach activities and will contribute to undergraduate and graduate research. Engagement of underrepresented students will occur during summer student internships. A collaboration with Italian Antarctic researchers, who have been studying the Terra Nova Bay ecosystem since the 1980s, aims to enhance the project and promote international scientific collaborations. The study will test whether a mutualistic symbioses between attached bacteria and Phaeocystis provides colonial cells a mechanism for alleviating chronic Vitamin B12 co-limitation effects thereby conferring them with a competitive advantage over diatom communities. The use of drifters in a time series study will provide the opportunity to track in both space and time a developing algal bloom in Terra Nova Bay and to determine community structure and the physiological nutrient status of microbial populations. A combination of flow cytometry, proteomics, metatranscriptomics, radioisotopic and stable isotopic labeling experiments will determine carbon and nutrient uptake rates and the role of bacteria in mitigating potential vitamin B12 and iron limitation. Membrane inlet and proton transfer reaction mass spectrometry will also be used to estimate net community production and release of volatile organic carbon compounds that are climatically active. Understanding how environmental parameters can influence microbial community dynamics in Antarctic coastal waters will advance an understanding of how changes in ocean stratification and chemistry could impact the biogeochemistry and food web dynamics of Southern Ocean ecosystems.
Microbial mats are found throughout the McMurdo Dry Valleys where summer snowmelt provides liquid water that allows these mats to flourish. Researchers have long studied the environmental conditions microbial mats need to grow. Despite these efforts, it has been difficult to develop a broad picture of these unique ecosystems. Recent advances in satellite technology now provide researchers an exciting new tool to study these special Antarctic ecosystems from space using the unique spectral signatures associated with microbial mats. This new technology not only offers the promise that microbial mats can be mapped and studied from space, this research will also help protect these delicate environments from potentially harmful human impacts that can occur when studying them from the ground. This project will use satellite imagery and spectroscopic techniques to identify and map microbial mat communities and relate their properties and distributions to both field and lab-based measurements. This research provides an exciting new tool to help document and understand the distribution of a major component of the Antarctic ecosystem in the McMurdo Dry Valleys. The goal of this project is to establish quantitative relationships between spectral signatures derived from orbit and the physiological status and biogeochemical properties of microbial mat communities in Taylor Valley, Antarctica, as measured by field and laboratory analyses on collected samples. The goal wioll be met by (1) refining atmospheric correction techniques using in situ radiometric rectification to derive accurate surface spectra; (2) collecting multispectral orbital images concurrent with in situ sampling and spectral measurements in the field to ensure temporal comparability; (3) measuring sediment, water, and microbial mat samples for organic and inorganic carbon content, essential biogeochemical nutrients, and chlorophyll-a to determine relevant mat characteristics; and (4) quantitatively associating these laboratory-derived characteristics with field-derived and orbital spectral signatures and parameters. The result of this work will be a more robust quantitative link between the distribution of microbial mat communities and their biogeochemical properties to landscape-scale spectral signatures. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
Methane is a potent greenhouse gas that is naturally emitted into the oceans by geologic seeps and microbial production. Based on studies of persistent deep-sea seeps at mid- and northern latitudes, researchers have learned that bacteria and archaea can create a "sediment filter" that oxidizes methane prior to its release. Antarctica is thought to contain large reservoirs of organic carbon buried beneath its ice which could a quantity of methane equivalent to all of the permafrost in the Arctic and yet we know almost nothing about the methane oxidizing microbes in this region. How these microbial communities develop and potentially respond to fluctuations in methane levels is an under-explored avenue of research. A bacterial mat was recently discovered at 78 degrees south, suggesting the possible presence of a methane seep, and associated microbial communities. This project will explore this environment in detail to assess the levels and origin of methane, and the nature of the microbial ecosystem present. An expansive bacterial mat appeared and/or was discovered at 78 degrees south in 2011. This site, near McMurdo Station Antarctica, has been visited since the mid-1960s, but this mat was not observed until 2011. The finding of this site provides an unusual opportunity to study an Antarctic marine benthic habitat with active methane cycling and to examine the dynamics of recruitment and community succession of seep fauna including bacteria, archaea, protists and metazoans. This project will collect the necessary baseline data to facilitate further studies of Antarctic methane cycling. The concentration and source of methane will be determined at this site and at potentially analogous sites in McMurdo Sound. In addition to biogeochemical characterization of the sites, molecular analysis of the microbial community will quantify the time scales on which bacteria and archaea respond to methane input and provide information on rates of community development and succession in the Southern Ocean. Project activities will facilitate the training of at least one graduate student and results will be shared at both local and international levels. A female graduate student will be mentored as part of this project and data collected will form part of her dissertation. Lectures will be given in K-12 classrooms in Oregon to excite students about polar science. National and international audiences will be reached through blogs and presentations at a scientific conference. The PI's previous blogs have been used by K-12 classrooms as part of their lesson plans and followed in over 65 countries.
Omelon, Christopher; Breecker, Daniel; Bennett, Philip
No dataset link provided
Cryptoendoliths are organisms that colonize microscopic cavities of rocks, which give them protection and allow them to inhabit extreme environments, such as the cold, arid desert of the Dry Valleys of Antarctica. Fossilized cryptoendoliths preserve the forms and features of organisms from the past and thus provide a unique opportunity to study the organisms? life histories and environments. To study this fossil record, there needs to be a better understanding of what environmental conditions allow these fossils to form. A climate gradient currently exists in the Dry Valleys that allows us to study living, dead, and fossilized cryptoendoliths from mild to increasingly harsh environments; providing insight to the limits of life and how these fossils are formed. This project will develop instruments to detect the biological activity of the live microorganisms and conduct laboratory experiments to determine the environmental limits of their survival. The project also will characterize the chemical and structural features of the living, dead, and fossilized cryptoendoliths to understand how they become fossilized. Knowing how microorganisms are preserved as fossils in cold and dry environments like Antarctica can help to refine methods that can be used to search for and identify evidence for extraterrestrial life in similar habitats on planets such as Mars. This project includes training of graduate and undergraduate students. Little is known about cryptoendolithic microfossils and their formation processes in cold, arid terrestrial habitats of the Dry Valleys of Antarctica, where a legacy of activity is discernible in the form of biosignatures including inorganic materials and microbial fossils that preserve and indicate traces of past biological activity. The overarching goals of the proposed work are: (1) to determine how rates of microbial respiration and biodegradation of organic matter control microbial fossilization; and (2) to characterize microbial fossils and their living counterparts to elucidate mechanisms for fossilization. Using samples collected across an increasingly harsher (more cold and dry) climatic gradient that encompasses living, dead, and fossilized cryptoendolithic microorganisms, the proposed work will: (1) develop an instrument to be used in the field that can measure small concentrations of CO2 in cryptoendolithic habitats in situ; (2) conduct incubation experiments to target variations in microbial activity in samples containing living, dead, and fossilized microorganisms as well as limits to microbial activity by measuring CO2 evolution and delta13C signatures; and (3) use a suite of microscopy techniques (CLSM, cryo-SEM, FIB-SEM, µ-XFM) to correlate laboratory experimental evidence for microbial viability and activity and to identify the chemical and morphological characteristics of biosignatures and microbial fossils. A metagenomic survey of microbial communities in these samples will be used to characterize differences in diversity, identify if specific microorganisms (e.g. prokaryotes, eukaryotes) are more capable of surviving under these harsh climatic conditions, and to corroborate microscopic observations of the viability states of these microorganisms.
Marine food webs can concentrate monomethylmercury (MMHg), a neurotoxin in mammals, in upper trophic level consumers. Despite their remoteness, coastal Antarctic marine ecosystems accumulate and biomagnify MMHg to levels observed at lower latitudes and in the Arctic. Marine sediments and other anoxic habitats in the oceans are typical areas where methylation of mercury occurs and these are likely places where MMHg is being produced. Krill, and more specifically their digestive tracts, may be a previously unaccounted for site where the production of MMHg may be occurring in the Antarctic. If monomethylmercury production is occurring in krill, current views regarding bioaccumulation in the food web and processes leading to the production and accumulation of mercury in the Antarctic Ocean could be better informed, if not transformed. This project will conduct a preliminary assessment of the krill gut microbiomes, the microbiome's genomic content and potential for production of monomethyl mercury by detecting the genes involved in mercury transformations. By analyzing the krill gut microbiome, the project will provide insights regarding animal-microbe interactions and their potential role in globally important biogeochemical cycles. This project will conduct a preliminary assessment of the krill gut microbiomes, the microbiomes genomic content and potential for production of monomethylmercury. The diversity and metabolic profiles of microorganisms in krill digestive tracts will be evaluated using massively parallel Illumina DNA sequencing technology to produce 16S rRNA gene libraries and assembled whole metagenomes. The project will also quantify the abundance and expression of Hg methylation genes, hgcAB, and identify their taxonomic affiliations in the microbiome communities. Environmental metagenomes, 16S rRNA gene inventories produced from this project will provide the polar science community with valuable databases and experimental tools with which to examine coastal Antarctic microbial ecology and biogeochemistry. The project will seek to provide a wider window into the diversity of extremophile microbial communities and the identification of potentially unique and useful bioactive compounds. In addition to public education and outreach. This project will train graduate students and provide educational and outreach opportunities at the participating institutions
This EAGER project will compare gene expression patterns in the planktonic communities under ice covers that form in coastal embayment's in the Antarctic Peninsula. Previous efforts taking advantage of unique ice conditions in November and December of 2015 allowed researchers to conduct an experiment to examine the role of sea ice cover on microbial carbon and energy transfer during the winter-spring transition. The EAGER effort will enable the researchers to conduct the "omics" analyses of the phytoplankton to determine predominant means by which energy is acquired and used in these settings. This EAGER effort will apply new expertise to fill an existing gap in ecological observations along the West Antarctic Peninsula. The principle product of the proposed work will be a novel dataset to be analyzed and by an early career researcher from an underserved community (veteran). The critical baseline data contained in this dataset enable a comparison of eukaryotic and prokaryotic gene expression patterns to establish the relative importance of chemoautotrophy, heterotrophy, mixotrophy, and phototrophy during the experiments. this information and data will be made immediately available to the broader scientific community, and will enable the development of further hypotheses on ecosystem change as sea ice cover changes in the region. Very little gene expression data is currently available for the Antarctic marine environment, and no gene expression data is available during the ecologically critical winter to spring transition. Moreover, ice cover in bays is common along the West Antarctic Peninsula yet the opportunity to study cryptophyte phytoplankton physiology beneath such ice conditions in coastal embayments is rare.
Recent discoveries of widespread liquid water and microbial ecosystems below the Antarctic ice sheets have generated considerable interest in studying Antarctic subglacial environments. Understanding subglacial hydrology, the persistence of life in extended isolation and the evolution and stability of subglacial habitats requires an integrated, interdisciplinary approach. The collaborative project, Minimally Invasive Direct Glacial Exploration (MIDGE) of the Biogeochemistry, Hydrology and Glaciology of Blood Falls, McMurdo Dry Valleys will integrate geophysical measurements, molecular microbial ecology and geochemical analyses to explore a unique Antarctic subglacial system known as Blood Falls. Blood Falls is a hypersaline, subglacial brine that supports an active microbial community. The subglacial brine is released from a crevasse at the surface of the Taylor Glacier providing an accessible portal into an Antarctic subglacial ecosystem. Recent geochemical and molecular analyses support a marine source for the salts and microorganisms in Blood Falls. The last time marine waters inundated this part of the McMurdo Dry Valleys was during the Late Tertiary, which suggests the brine is ancient. Still, no direct samples have been collected from the subglacial source to Blood Falls and little is known about the origin of this brine or the amount of time it has been sealed below Taylor Glacier. Radar profiles collected near Blood Falls delineate a possible fault in the subglacial substrate that may help explain the localized and episodic nature of brine release. However it remains unclear what triggers the episodic release of brine exclusively at the Blood Falls crevasse or the extent to which the brine is altered as it makes its way to the surface. The MIDGE project aims to determine the mechanism of brine release at Blood Falls, evaluate changes in the geochemistry and the microbial community within the englacial conduit and assess if Blood Falls waters have a distinct impact on the thermal and stress state of Taylor Glacier, one of the most studied polar glaciers in Antarctica. The geophysical study of the glaciological structure and mechanism of brine release will use GPR, GPS, and a small passive seismic network. Together with international collaborators, the 'Ice Mole' team from FH Aachen University of Applied Sciences, Germany (funded by the German Aerospace Center, DLR), MIDGE will develop and deploy innovative, minimally invasive technologies for clean access and brine sample retrieval from deep within the Blood Falls drainage system. These technologies will allow for the collection of samples of the brine away from the surface (up to tens of meters) for geochemical analyses and microbial structure-function experiments. There is concern over the contamination of pristine subglacial environments from chemical and biological materials inherent in the drilling process; and MIDGE will provide data on the efficacy of thermoelectric probes for clean access and retrieval of representative subglacial samples. Antarctic subglacial environments provide an excellent opportunity for researching survivability and adaptability of microbial life and are potential terrestrial analogues for life habitats on icy planetary bodies. The MIDGE project offers a portable, versatile, clean alternative to hot water and mechanical drilling and will enable the exploration of subglacial hydrology and ecosystem function while making significant progress towards developing technologies for minimally invasive and clean sampling of icy systems.
This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5). The LISSARD project (Lake and Ice Stream Subglacial Access Research Drilling) is one of three research components of the WISSARD integrative initiative (Whillans Ice Stream Subglacial Access Research Drilling) that is being funded by the Antarctic Integrated System Science Program of NSF's Office of Polar Programs, Antarctic Division. The overarching scientific objective of WISSARD is to assess the role of water beneath a West Antarctic ice stream in interlinked glaciological, geological, microbiological, geochemical, and oceanographic systems. The LISSARD component of WISSARD focuses on the role of active subglacial lakes in determining how fast the West Antarctic ice sheet loses mass to the global ocean and influences global sea level changes. The importance of Antarctic subglacial lakes has only been recently recognized, and the lakes have been identified as high priority targets for scientific investigations because of their unknown contributions to ice sheet stability under future global warming scenarios. LISSARD has several primary science goals: A) To provide an observational basis for improving treatments of subglacial hydrological and mechanical processes in models of ice sheet mass balance and stability; B) To reconstruct the past history of ice stream stability by analyzing archives of past basal water and ice flow variability contained in subglacial sediments, porewater, lake water, and basal accreted ice; C) To provide background understanding of subglacial lake environments to benefit RAGES and GBASE (the other two components of the WISSARD project); and D) To synthesize data and concepts developed as part of this project to determine whether subglacial lakes play an important role in (de)stabilizing Antarctic ice sheets. We propose an unprecedented synthesis of approaches to studying ice sheet processes, including: (1) satellite remote sensing, (2) surface geophysics, (3) borehole observations and measurements and, (4) basal and subglacial sampling. <br/><br/>INTELLECTUAL MERIT: The latest report of the Intergovernmental Panel on Climate Change recognized that the greatest uncertainties in assessing future global sea-level change stem from a poor understanding of ice sheet dynamics and ice sheet vulnerability to oceanic and atmospheric warming. Disintegration of the WAIS (West Antarctic Ice Sheet) alone would contribute 3-5 m to global sea-level rise, making WAIS a focus of scientific concern due to its potential susceptibility to internal or ocean-driven instability. The overall WISSARD project will test the overarching hypothesis that active water drainage connects various subglacial environments and exerts major control on ice sheet flow, geochemistry, metabolic and phylogenetic diversity, and biogeochemical transformations. <br/><br/>BROADER IMPACTS: Societal Relevance: Global warming, melting of ice sheets and consequential sea-level rise are of high societal relevance. Science Resource Development: After a 9-year hiatus WISSARD will provide the US-science community with a renewed capability to access and study sub-ice sheet environments. Developing this technological infrastructure will benefit the broader science community and assets will be accessible for future use through the NSF-OPP drilling contractor. Furthermore, these projects will pioneer an approach implementing recommendations from the National Research Council committee on Principles of Environmental Stewardship for the Exploration and Study of Subglacial Environments (2007). Education and Outreach (E/O): These activities are grouped into four categories: i) increasing student participation in polar research by fully integrating them in our research programs; ii) introducing new investigators to the polar sciences by incorporating promising young investigators in our programs, iii) promotion of K-12 teaching and learning programs by incorporating various teachers and NSTA programs, and iv) reaching a larger public audience through such venues as popular science magazines, museum based activities and videography and documentary films. In summary, WISSARD will promote scientific exploration of Antarctica by conveying to the public the excitement of accessing and studying what may be some of the last unexplored aquatic environments on Earth, and which represent a potential analogue for extraterrestrial life habitats on Europa and Mars.
The McMurdo Dry Valleys in Antarctica are recognized as being the driest, coldest and probably one of the harshest environments on Earth. In addition to the lack of water, the biota in the valleys face a very limited supply of nutrients such as nitrogen compounds - necessary for protein synthesis. The glacial streams of the Dry Valleys have extensive cyanobacterial (blue green algae) mats that are a major source of carbon and nitrogen compounds to biota in this region. While cyanobacteria in streams are important as a source of these compounds, other non-photosynthetic bacteria also contribute a significant fraction (~50%) of fixed nitrogen compounds to valley biota. This research effort will involve an examination of exactly which non-phototrophic bacteria are involved in nitrogen fixation and what environmental factors are responsible for controlling nitrogen fixation by these microbes. This work will resolve the environmental factors that control the activity, abundance and diversity of nitrogen-fixing microbes across four of the McMurdo Dry Valleys. This will allow for comparisons among sites of differing latitude, temperature, elevation and exposure to water. These results will be integrated into a landscape wetness model that will help determine the impact of both cyanobacterial and non-photosynthetic nitrogen fixing microorganisms in this very harsh environment. The Dry Valleys in many ways resemble the Martian environment, and understanding the primitive life and very simple nutrient cycling in the Dry Valleys has relevance for understanding how life might have once existed on other planets. Furthermore, the study of microbes from extreme environments has resulted in numerous biotechnological applications such as the polymerase chain reaction for amplifying DNA and mechanisms for freeze resistance in agricultural crops. Thus, this research should yield insights into how biota survive in extreme environments, and these insights could lead to other commercial applications.
This CAREER proposal will support an early career female PI to establish an integrated research and education program in the fields of polar biology and environmental microbiology, focusing on single-celled eukaryotes (protists) in high latitude ice-covered Antarctic lakes systems. Protists play important roles in energy flow and material cycling, and act as both primary producers (fixing inorganic carbon by photosynthesis) and consumers (preying on bacteria by phagotrophic digestion). The McMurdo Dry Valleys (MDV) located in Victoria Land, Antarctica, harbor microbial communities which are isolated in the unique aquatic ecosystem of perennially ice-capped lakes. The lakes support exclusively microbial consortia in chemically stratified water columns that are not influenced by seasonal mixing, allochthonous inputs, or direct human impact. This project will exploit permanently stratified biogeochemistry that is unique across the water columns of several MDV lakes to address gaps in our understanding of protist trophic function in aquatic food webs. The proposed research will examine (1) the impact of permanent biogeochemical gradients on protist trophic strategy, (2) the effect of major abiotic drivers (light and nutrients) on the distribution of two key mixotrophic and photoautotrophic protist species, and (3) the effect of episodic nutrient pulses on mixotroph communities in high latitude (ultraoligotrophic) MDV lakes versus low latitude (eutrophic) watersheds. The project will impact the fields of microbial ecology and environmental microbiology by combining results from field, laboratory and in situ incubation studies to synthesize new models for the protist trophic roles in the aquatic food web. The research component of this proposed project will be tightly integrated with the development of two new education activities designed to exploit the inherent excitement associated with polar biological research. The educational objectives are: 1) to establish a teaching module in polar biology in a core undergraduate course for microbiology majors; 2) to develop an instructional module to engage middle school girls in STEM disciplines. Undergraduates and middle school girls will also work with a doctoral student on his experiments in local Ohio watersheds.
Like no other region on Earth, the northern Antarctic Peninsula represents a spectacular natural laboratory of climate change and provides the opportunity to study the record of past climate and ecological shifts alongside the present-day changes in one of the most rapidly warming regions on Earth. This award supports the cryospheric and oceano-graphic components of an integrated multi-disciplinary program to address these rapid and fundamental changes now taking place in Antarctic Peninsula (AP). By making use of a marine research platform (the RV NB Palmer and on-board helicopters) and additional logistical support from the Argentine Antarctic program, the project will bring glaciologists, oceanographers, marine geologists and biologists together, working collaboratively to address fundamentally interdisciplinary questions regarding climate change. The project will include gathering a new, high-resolution paleoclimate record from the Bruce Plateau of Graham Land, and using it to compare Holocene- and possibly glacial-epoch climate to the modern period; investigating the stability of the remaining Larsen Ice Shelf and rapid post-breakup glacier response ? in particular, the roles of surface melt and ice-ocean interactions in the speed-up and retreat; observing the contribution of, and response of, oceanographic systems to ice shelf disintegration and ice-glacier interactions. Helicopter support on board will allow access to a wide range of glacial and geological areas of interest adjacent to the Larsen embayment. At these locations, long-term in situ glacial monitoring, isostatic uplift, and ice flow GPS sites will be established, and high-resolution ice core records will be obtained using previously tested lightweight drilling equipment. Long-term monitoring of deep water outflow will, for the first time, be integrated into changes in ice shelf extent and thickness, bottom water formation, and multi-level circulation by linking near-source observations to distal sites of concentrated outflow. The broader impacts of this international, multidisciplinary effort are that it will significantly advance our understanding of linkages amongst the earth's systems in the Polar Regions, and are proposed with international participation (UK, Spain, Belgium, Germany and Argentina) and interdisciplinary engagement in the true spirit of the International Polar Year (IPY). It will also provide a means of engaging and educating the public in virtually all aspects of polar science and the effects of ongoing climate change. The research team has a long record of involving undergraduates in research, educating high-performing graduate students, and providing innovative and engaging outreach products to the K-12 education and public media forums. Moreover, forging the new links both in science and international Antarctic programs will provide a continuing legacy, beyond IPY, of improved understanding and cooperation in Antarctica.
The research will examine how diatoms (an important group of plankton in the Southern Ocean) adapt to environmental change. Diatoms will be sampled from different regions of the Southern Ocean, including the Drake Passage, the Pacific Sector of the Southern Ocean and the Ross Sea and examined to determine the range of genetic variation among diatoms in these regions. Experiments on a range of diatoms will be conducted in home laboratories and will be aimed at measuring shifts in physiological capacities over many generations in response to directional changes in the environment (temperature and pH). The information on the genetic diversity of field populations combined with information on potential rates of adaptability and genome changes will provide insight into ways in which polar marine diatoms populations may respond to environmental changes that may occur in surface oceans in the future or may have occurred during past climate conditions. Such information allows better modeling of biogeochemical cycles in the ocean as well as improves our abilities to interpret records of past ocean conditions. The project will support a doctoral student and a postdoctoral researcher as well as several undergraduate students. These scientists will learn the fundamentals of experimental evolution, a skill set that is being sought in the fields of biology and oceanography. The project also includes a collaboration with the Metcalf Institute for Marine and Environmental Reporting that will design and facilitate a session focused on current research related to evolution and climate change to be held at the annual conference of the National Association of Science Writers (NASW). Both physiological and genetic variation are key parameters for understanding evolutionary processes in phytoplankton but they are essentially unknown for Southern Ocean diatoms. The extent to which these two factors determine plasticity and adaptability in field populations and the interaction between them will influence how and whether cold-adapted diatoms can respond to changing environments. This project includes a combination of field work to identify genetic diversity within diatoms using molecular approaches and experiments in the lab to assess the range of physiological variation in contemporary populations of diatoms and evolution experiments in the lab to assess how the combination of genetic diversity and physiological variation influence the evolutionary potential of diatoms under a changing environment. This research will uncover general relationships between physiological variation, genetic diversity, and evolutionary potential that may apply across microbial taxa and geographical regions, substantially improving efforts to predict shifts in marine ecosystems. Results from this study can be integrated into developing models that incorporate evolution to predict ecosystem changes under future climate change scenarios.
Intellectual Merit: The MCM-SkyTEM project mapped resistivity in the McMurdo Dry Valleys and at Cape Barne on the Ross Island during the 2011-12 austral season using an airborne transient electromagnetic method. The SkyTEM system is mounted to a helicopter enabling a broad geophysical survey of subsurface resistivity structure over terrain that is inaccessible to traditional ground-based methods. Resistivity measurements obtained distinguish between highly resistive geologic materials such as glacier ice, bedrock and permafrost, and conductive materials such as unfrozen sediments or permafrost with liquid brine to depths of about 300 m. The PIs request funding to derive data products relevant to physical and chemical conditions in potential subsurface microbial habitats of the McMurdo Dry Valleys, similar cold regions on Earth and other planetary bodies. They will use these data products to characterize the hydrologic history of McMurdo Dry Valleys as well as the subsurface hydrologic connectivity in the region to investigate the implications for nutrient and microbial transport. The PIs will make these data products accessible to the research community. Broader impacts: Polar microbial habitats are of high societal and scientific interest because they represent important testing grounds for the limits of life on Earth and other planetary bodies. Project deliverables will include teaching aids for undergraduate and graduate students. Two Ph.D. students will obtain advanced research training as part of this project. The PIs and students on this project will also engage in informal public outreach opportunities by presenting at local K-12 schools and reaching out to local media outlets on stories relating to SkyTEM research.
The biota of the world's seafloor is fueled by bursts of seasonal primary production. For food-limited sediment communities to persist, a balance must exist between metazoan consumption of and competition with bacteria, a balance which likely changes through the seasons. Polar marine ecosystems are ideal places to study such complex interactions due to stark seasonal shifts between heterotrophic and autotrophic communities, and temperatures that may limit microbial processing of organic matter. The research will test the following hypotheses: 1) heterotrophic bacteria compete with macrofauna for food; 2) as phytoplankton populations decline macrofauna increasingly consume microbial biomass to sustain their populations; and 3) in the absence of seasonal photosynthetic inputs, macrofaunal biodiversity will decrease unless supplied with microbially derived nutrition. Observational and empirical studies will test these hypotheses at McMurdo Station, Antarctica, where a high-abundance macro-infaunal community is adapted to this boom-and-bust cycle of productivity. The investigator will mentor undergraduates from a predominantly minority-serving institution, in the fields of invertebrate taxonomy and biogeochemistry. The general public and young scientists will be engaged through lectures at local K-12 venues and launch of an interactive website. The results will better inform scientists and managers about the effects of climate change on polar ecosystems and the mechanisms of changing productivity patterns on global biodiversity.
The Western Antarctic Peninsula (WAP) has experienced unprecedented warming and shifts in sea ice cover over the past fifty years. How these changes impact marine microbial communities, and subsequently how these shifts in the biota may affect the carbon cycle in surface waters is unknown. This work will examine how these ecosystem-level changes affect microbial community structure and function. This research will use modern metagenomic and transcriptomic approaches to test the hypothesis that the introduction of organic matter from spring phytoplankton blooms drives turnover in microbial communities. This research will characterize patterns in bacterial and archaeal succession during the transition from the austral winter at two long-term monitoring sites: Palmer Station in the north and Rothera Station in the south. This project will also include microcosm incubations to directly assess the effects of additions of organic carbon and melted sea ice on microbial community structure and function. The results of this work will provide a broader understanding of the roles of both rare and abundant microorganisms in carbon cycling within the WAP region, and how these communities may shift in structure and function in response to climate change. Results will be widely disseminated through publications as well as through presentations at national and international meetings. The research will provide training opportunities for both graduate and undergraduate students and will enhance international collaborations with the British Antarctic Survey.
Hastings/1246223 This award supports a project with the aim of distinguishing the sources of nitrate deposition to the West Antarctic Ice Sheet (WAIS) using isotopic ratios snow in archive snow and ice samples. The isotopic composition of nitrate has been shown to contain information about the source of the nitrate (i.e. nitrogen oxides = NOx = NO+NO2) and the oxidation processes that convert NOx to nitrate in the atmosphere prior to deposition. A difficulty in interpreting records in the context of NOx sources is that nitrate can be post-depositionally processed in surface snow, such that the archived record does not reflect the composition of the atmosphere. This intellectual merit of this work specifically aims to investigate variability in the isotopic composition of nitrate in snow and ice from the WAIS in the context of accumulation rate, NOx source emissions, and atmospheric chemistry. These records will be interpreted in the context of our understanding of biospheric (biomass burning, microbial processes in soils), atmospheric (lightning, transport, chemistry), and climate (temperature, accumulation rate) changes over time. A graduate student will be supported as part of this project, and both graduate student and PI will be involved in communicating the utility and results of polar research to elementary school students in the Providence, RI area. The broader impacts of the project also include making efforts to attract more young, female scientists to polar research by establishing a connection between the Earth Science Women's Network (ESWN), an organization PI Hastings helped to establish, and the Association of Polar Early Career Scientists (APECS). Finally, results of all measurements will be presented at relevant conferences, made available publicly and published in peer-reviewed journals.
Uncovering the dynamics of dissolved organic matter (DOM) is central to an understanding of the global carbon cycle, as organic material from lakes, streams, oceans and soils passes through this pool. DOM acts as a key energy source for microbes in many ecosystems and therefore can affect regional nutrient cycling patterns. For example, preliminary results suggest that organisms isolated from a supraglacial stream on Cotton Glacier, Antarctica, may be important in DOM cycling in this relatively simple, low temperature system. However, little is known about the functional attributes of the microbes that interact with DOM in the environment. This project will use state-of-the-art genomics, proteomics and metabolomics approaches to understand the mechanisms by which two microbial isolates, CG3 and CG9_1, affect DOM cycling. Liquid chromatography-mass spectrometry will also be used to better characterize the microbially-derived DOM from this ecosystem. This project will support the research and training of one undergraduate and two graduate students. Results will be widely disseminated through publications as well as through presentations at national and international meetings. In addition, raw data will be made available through open-access databases. Understanding the relationship between cold-adapted microbial metabolisms and DOM pools is important as more than 90% of the Earth?s oceans are below 5 degrees Celsius.
EAGER: Collaborative Research: Habitability of Antarctic lakes and detectability of microbial life in icy environments by autonomous year-round instrumentation, is supported by the Antarctic Integrated System Science (AISS) and the Antarctic Organisms and Ecosystems (AOE) programs within the Antarctic Sciences section in the Division of Polar Programs within the Geosciences Directorate of the National Sciences Foundation (NSF). The funds will allow the measurement of year-round properties of the microbes and the surrounding water in Lake Bonney, a lake with four meters of permanent ice cover over forty meters of liquid water in the Dry Valleys of Antarctica. NSF funds will be used to support the deployment, and the science enabled by the deployment, and NASA (the National Aeronautics and Space Administration) funds will be used to purchase the equipment. Intellectual Merit: This research will be the first to make year-round measurements of the microbial community, and several associated environmental variables, in the continuously liquid portions of Lake Bonney, Antarctica. Three different types of equipment will be deployed in each of the lobes of Lake Bonney. The first instrument is an ITP (an ice-tethered profiler) that will measure physical parameters such as temperature, dissolved oxygen, and chlorophyll throughout the full depth of the liquid water portion of the lake, making measurements at least once each week. The second and third instruments will be used to collect discrete water samples at least every two weeks to determine A) the biological community (assessing metabolic and phylogenetic diversity) and B) the geochemistry (e.g., dissolved organic carbon, and dissolved inorganic nitrogen species). Such samplers have never been used to measure these properties year-round in the Antarctic. Cold temperatures, bottom lake water salinities that are four times greater than the ocean, the thick permanent ice cover, and the lack of sunlight to recharge batteries all present significant challenges for the project, thus classifying the work as an early, high-risk, high-reward activity (the acronym EAGER stands for Early-concept Grants for Exploratory Research). Broader Impacts: There is much interest in understanding the ecosystems of the Polar regions in an era of climate change. Logistical limitations dictate much of this work only take place in the summer, until new autonomous technologies can open the door for year-round measurements. This award will be the first to attempt year-round microbial sampling in Antarctica. The McMurdo Dry Valleys region is also the site of a Long-Term Ecological Research (LTER) Program, and the research conducted on this project with benefit from, and contribute to, the larger LTER project. The instruments used in the project will be purchased by NASA, so two separate agencies have agreed to explore the feasibility of an early stage project. There will be at least three graduate student trained during the project, and the team will also participate in outreach activities at several venues including the Crow Reservation in Montana.
The McMurdo Dry Valleys in Antarctica are among the coldest, driest habitats on the planet. Previous research has documented the presence of surprisingly diverse microbial communities in the soils of the Dry Valleys despite these extreme conditions. However, the degree to which these organisms are active is unknown; it is possible that much of this diversity reflects microbes that have blown into this environment that are subsequently preserved in these cold, dry conditions. This research will use modern molecular techniques to answer a fundamental question regarding these communities: which organisms are active and how do they live in such extreme conditions? The research will include manipulations to explore how changes in water, salt and carbon affect the microbial community, to address the role that these organisms play in nutrient cycling in this environment. The results of this work will provide a broader understanding of how life adapts to such extreme conditions as well as the role of dormancy in the life history of microorganisms. Results will be widely disseminated through publications as well as through presentations at national and international meetings; raw data will be made available through a high-profile web-based portal. The research will support two graduate students, two undergraduate research assistants and a postdoctoral fellow. The results will be incorporated into a webinar targeted to secondary and post-secondary educators and a complimentary hands-on class activity kit will be developed and made available to various teacher and outreach organizations.
This award supports a detailed, molecular level characterization of dissolved organic carbon and microbes in Antarctic ice cores. Using the most modern biological (genomic), geochemical techniques, and advanced chemical instrumentation researchers will 1) optimize protocols for collecting, extracting and amplifying DNA from deep ice cores suitable for use in next generation pyrosequencing; 2) determine the microbial diversity within the ice core; and 3) obtain and analyze detailed molecular characterizations of the carbon in the ice by ultrahigh resolution Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS). With this pilot study investigators will be able to quantify the amount of material (microbial biomass and carbon) required to perform these characterizations, which is needed to inform future ice coring projects. The ultimate goal will be to develop protocols that maximize the yield, while minimizing the amount of ice required. The broader impacts include education and outreach at both the local and national levels. As a faculty mentor with the American Indian Research Opportunities and BRIDGES programs at Montana State University, Foreman will serve as a mentor to a Native American student in the lab during the summer months. Susan Kelly is an Education and Outreach Coordinator with a MS degree in Geology and over 10 years of experience in science outreach. She will coordinate efforts for comprehensive educational collaboration with the Hardin School District on the Crow Indian Reservation in South-central Montana.
Advances in molecular techniques have expanded our understanding of soil microbial communities, and raised important questions about regional and global patterns in microbial diversity. The proposed research will investigate the composition and activity of microbial communities across a range of geochemical and hydrologic soil conditions, and over local to regional scales in the Transantarctic Mountains, in order to assess controls over microbial biogeography. The research targets two areas in the Transantarctic mountains, the McMurdo Dry Valleys, and the Beardmore Glacier region further south, the latter representing an underexplored and inarguably more extreme soil environment. The research project will adopt an integrated approach, using molecular techniques and in situ assessment of biological activity in a quantitative biogeographical framework, with the goal of distinguishing fine versus broad scale controls over microbial community structure. The research is essential to determining the basic trophic status of extreme microbial food webs, and their sensitivity to climate change. The investigators will engage secondary and post-secondary educators through first person outreach as well as web-based communications and exercises. Two postdoctoral scientists will be trained in an interdisciplinary and international setting.
ASPIRE is an NSF-funded project that will examine the ecology of the Amundsen Sea during the Austral summer of 2010. ASPIRE includes an international team of trace metal and carbon chemists, phytoplankton physiologists, microbial and zooplankton ecologists, and physical oceanographers, that will investigate why and how the Amundsen Sea Polynya is so much more productive than other polynyas and whether interannual variability can provide insight to climate-sensitive mechanisms driving carbon fluxes. This project will compliment the existing ASPIRE effort by using 1) experimental manipulations to understand photoacclimation of the dominant phytoplankton taxa under conditions of varying light and trace metal abundance, 2) nutrient addition bioassays to determine the importance of trace metal versus nitrogen limitation of phytoplankton growth, and 3) a numerical ecosystem model to understand the importance of differences in mixing regime, flow field, and Fe sources in controlling phytoplankton bloom dynamics and community composition in this unusually productive polynya system. The research strategy will integrate satellite remote sensing, field-based experimental manipulations, and numerical modeling. Outreach and education include participation in Stanford's Summer Program for Professional Development for Science Teachers, Stanford's School of Earth Sciences high school internship program, and development of curriculum for local science training centers, including the Chabot Space and Science Center. Undergraduate participation and training will include support for both graduate students and undergraduate assistants.
Dissolved organic matter (DOM) comprises a significant pool of Earth's organic carbon that dwarfs the amount present in living aquatic organisms. The properties and reactivity of DOM are not well defined, and the evolution of autochthonous DOM from its precursor materials in freshwater has not been observed. Recent sampling of a supraglacial stream formed on the Cotton Glacier in the Transantarctic Mountains revealed DOM that more closely resembles an assemblage of recognizable precursor organic compounds, based upon its UV-VIS and fluorescence spectra. It is suggested that the DOM from this water evolved over time to resemble materials present in marine and many inland surface waters. The transient nature of the system i.e., it reforms seasonally, also prevents any accumulation of the refractory DOM present in most surface waters. Thus, the Cotton Glacier provides us with a unique environment to study the formation of DOM from precursor materials. An interdisciplinary team will study the biogeochemistry of this progenitor DOM and how microbes modify it. By focusing on the chemical composition of the DOM as it shifts from precursor material to the more humified fractions, the investigators will relate this transition to bioavailability, enzymatic activity, community composition and microbial growth efficiency. This project will support education at all levels, K-12, high school, undergraduate, graduate and post-doc and will increase participation by under-represented groups in science. Towards these goals, the investigators have established relationships with girls' schools and Native American programs. Additional outreach will be carried out in coordination with PolarTREC, PolarPalooza, and if possible, an Antarctic Artist and Writer.
The relatively pristine Antarctic continent with its extensive maritime zone represents a unique location on the planet to investigate the long distance aerial transport and deposition of marine microorganisms. The vast extent of new sea ice that forms each winter around the continent results in large numbers of frost flowers, delicate ice-crystal structures of high salt content that form on the surface of the ice and are readily dispersed by wind. The proposed research builds on earlier work in the Arctic and tests the new hypothesis that wind-borne frost flowers provide an effective mechanism for the transport of marine bacteria over long distances, one that can be uniquely sourced and tracked by the frost flower salt signature in the Antarctic realm. A highly resolved genomic snapshot of the microbial community will be acquired at each stage in the transport path, which will track decreasing fractions of the marine microbial community as it freezes into sea ice, incorporates into frost flowers, converts to aerosols, and ultimately deposits within continental snowpack. En route from sea ice to snowpack, marine bacteria will be exposed to an array of environmental stresses, including high salinity, low temperatures, UV light and potential desiccation. A parallel proteomic analysis will enable an evaluation of the microbial response to these extreme conditions and potential survival mechanisms that allow persistence or eventual colonization of deposition sites across Antarctica. Current understanding of microbes in the Antarctic atmosphere is based on a limited number of microscopic and culture-based assays and a single report of low-resolution 16S RNA gene sequence analysis. The research will broadly impact understanding of atmospheric microbiology, from source to deposition, and various issues of microbial survival, colonization, endemism, and diversity under extreme conditions. In addition to venues that reach the scientific community, the research team will develop a permanent multi-media and artifact-based exhibit on Antarctic Microbial Transport that will be showcased at Seattle's Pacific Science Center (PSC), which educates nearly a million visitors annually.
Intellectual Merit: The PI proposes to utilize computer models used by hydrogeologists to establish the fate and transport of contamination and determine the extent of drilling fluid contamination in the ANDRILL SMS core. For these models, previously collected logs of lithology, porosity, fracture density, fracture type, fracture orientation, drilling fluid loss, drilling fluid characteristics and temperature will be used as input parameters. In addition, biodegradation and sorption constants for the drilling fluid will be determined and incorporated into the models. Samples of drilling fluids used during coring as well as the return fluids were collected at the drill site using standard microbiological sampling techniques. Fluids will be tested at in situ temperatures under aerobic and anaerobic conditions to determine biodegradation constants. Sorption will be determined between the drilling fluids and core samples using standard isotherm methods. Geochemical and microbial fingerprints of the fluids and the changes during biodegradation will determine the potential impact of the drilling fluids on the isolated microbial communities and the geochemistry within various subsurface lithologic units beneath the southern McMurdo Sound in Antarctica. The results of this study could potentially provide guidelines on developing less detrimental methods for future exploration, if deemed necessary through this research. Broader impacts: This proposed project will train a graduate student. The methods developed for analyses of samples in this project will serve as a guide for future studies of similar interest and will improve the understanding of ecological impacts of geologic drilling in Antarctica. The results of this study will be used as a reference for comparison with future studies examining newly developed, and improved, sample collection methods in future exploratory drilling projects in pristine environments. The PI is new to Antarctic research.
This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).<br/><br/>Light quality and availability are likely to change in polar ecosystems as ice coverage and thickness decrease. How microbes adjust to these and other changes will have huge impacts on the polar marine ecosystems. Little is known about photoheterotrophic prokaryotes, which are hypothesized to gain a metabolic advantage by harvesting light energy in addition to utilizing dissolved organic matter (DOM). Photoheterotrophy is not included in current models of carbon cycling and energy flow. This research will examine three questions: 1. Are photoheterotrophic microbes present and active in Antarctic waters in winter and summer? 2. Does community structure of photoheterotrophs shift between summer and winter? 3. Which microbial groups assimilate more DOM in light than in the dark? The research will test hypotheses about activity of photoheterotrophs in winter and in summer, shifts in community structure between light and dark seasons and the potentially unique impacts of photoheterotrophs on biogeochemical processes in the Antarctic. The project will directly support a graduate student, will positively impact the NSF REU program at the College of Marine and Earth Studies, and will include students from the nation?s oldest historical minority college. The results will be featured during weekly tours of Lewes facilities (about 1000 visitors per year) and during Coast Day, an annual open-house that attracts about 10,000 visitors.
Lake Vida is the largest lake of the McMurdo Dry Valleys, with an approximately 20 m ice cover overlaying a brine of unknown depth with at least 7 times seawater salinity and temperatures below -10 degrees C year-round. Samples of brine collected from ice above the main water body contain 1) the highest nitrous oxide levels of any natural water body on Earth, 2) unusual geochemistry including anomalously high ammonia and iron concentrations, 3) high microbial counts with an unusual proportion (99%) of ultramicrobacteria. The microbial community is unique even compared to other Dry Valley Lakes. The research proposes to enter, for the first time the main brine body below the thick ice of Lake Vida and perform in situ measurements, collect samples of the brine column, and collect sediment cores from the lake bottom for detailed geochemical and microbiological analyses. The results will allow the characterization of present and past life in the lake, assessment of modern and past sedimentary processes, and determination of the lake's history. The research will be conducted by a multidisciplinary team that will uncover the biogeochemical processes associated with a non-photosynthetic microbial community isolated for a significant period of time. This research will address diversity, adaptive mechanisms and evolutionary processes in the context of the physical evolution of the environment of Lake Vida. Results will be widely disseminated through publications, presentations at national and international meetings, through the Subglacial Antarctic Lake Exploration (SALE) web site and the McMurdo LTER web site. The research will support three graduate students and three undergraduate research assistants. The results will be incorporated into a new undergraduate biogeosciences course at the University of Illinois at Chicago which has an extremely diverse student body, dominated by minorities.
The glacial streams of the McMurdo Dry Valleys have extensive cyanobacterial mats that are a probable source of fixed C and N to the Valleys. The research will examine the interplay between the microbial mats in the ephemeral glacial streams and the microbiota of the hyporheic soils (wetted soil zone) underlying and adjacent to those mats. It is hypothesized that the mats are important sources of organic carbon and fixed nitrogen for the soil communities of the hyporheic zone, and release dissolved organic carbon (DOC) and nitrogen (DON) that serves the entire Dry Valley ecosystem. Field efforts will entail both observational and experimental components. Direct comparisons will be made between the mats and microbial populations underlying naturally rehydrated and desiccated mat areas, and between mat areas in the melt streams of the Adams and Miers Glaciers in Miers Valley. Both physiological and phylogenetic indices of the soil microbiota will be examined. Observations will include estimates of rates of mat carbon and nitrogen fixation, soil respiration and leucine and thymidine uptake (as measures of protein & DNA synthesis, respectively) by soil bacteria, bacterial densities and their molecular ecology. Experimental manipulations will include experimental re-wetting of soils and observations of the time course of response of the microbial community. The research will integrate modern molecular genetic approaches (ARISA-DNA fingerprinting and ultra deep 16S rDNA microbial phylogenetic analysis) with geochemistry to study the diversity, ecology, and function of microbial communities that thrive in these extreme environments. The broader impacts of the project include research and educational opportunities for graduate students and a postdoctoral associate. The P.I.s will involve undergraduates as work-study students and in REU programs, and will participate in educational and outreach programs.
This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).<br/><br/>Two models have been proposed to describe controls over microbial biogeography. One model proposes that microbes are ubiquitously distributed across the global environment, and that environmental conditions select for taxa physiologically adapted to local physical conditions. An alternative model predicts that dispersal is the important limitation to the distribution of microorganisms and that spatial heterogeneity of microbial communities is a result of both dispersal and local environmental limitations. According to both models, spatial heterogeneity of microbial communities may be especially pronounced in extreme ecosystems where the environmental selection for organisms with suitable physiology is most strongly manifest. We propose that Antarctic terrestrial environments are ideal places to examine microbial biogeography for 3 reasons: 1) the pristine nature and remoteness of Antarctica minimizes the prevalence of exotic species dispersed through human vectors; 2) the extreme conditions of Antarctic environments provide a strong environmental filter which limits the establishment of non-indigenous taxa; and 3) extreme heterogeneity in the terrestrial environment provides natural gradients of soil conditions (temperature, water and nutrient availability). In the proposed research we will investigate the influence of snow on the composition and spatial distribution of soil microbial communities and linked biogeochemical cycling in the McMurdo Dry Valleys. We will conduct fieldwork at the landscape scale (repeated remote sensing to characterize snow distribution), at the valley and patch scales (quantify snow patch ablation, microbial communities and biogeochemical cycling in subnivian soils). We hypothesize that snow patches play an important role in structuring the spatial distribution of soil microbial communities and their associated ecosystem functioning because of the physical and hydrological influences that snow patches have on the soil environment. The research will contribute to greater public awareness of the importance of polar research to fundamental questions of biology, ecology and hydrology through direct linkages with International Antarctic Institute public outreach activities, including dissemination of web-based learning units on environmental science and microbiology, targeted as resources for secondary and post-secondary educators. Three graduate students, one postdoctoral scholar and multiple undergraduates will participate in the research activities.
The mechanisms enabling bacteria to be metabolically active at very low temperatures are of considerable importance to polar microbial ecology, astrobiology, climate and cryopreservation. This research program has two main objectives. The first is to investigate metabolic activities and gene expression of polar marine psychrophilic bacteria when confronted with freezing conditions at temperatures above the eutectic of seawater (<54C) to unveil cold adaptation mechanisms with relevance to wintertime sea-ice ecology. The second objective is to discern if psychrophilic processes of leucine incorporation into proteins, shown to occur to -196C, amount to metabolic activity providing for the survival of cells or are merely biochemical reactions still possible in flash-frozen samples without any effect on survival. We will examine extracellular and intracellular processes of psychrophilic activity above and below the eutectic by (i) determining the temperature range of metabolic activities such as DNA synthesis, carbon utilization, respiration and ATP generation using radioactive tracer technology, including a control at liquid helium temperature (-268.9C), (ii) analyzing gene expression in ice using whole genome and microarray analyses and iii) examining the role of exopolymeric substances (EPS) and ice micro-physics for the observed activity using an in-situ microscopy technique. Results of the proposed research can be expected to aid in the determination of cellular and genetic strategies that allow cells to maintain activity at extremely low temperatures within an icy matrix and/or to resume activity again when more growth-permissive conditions are encountered. The research is an interdisciplinary collaboration involving three different institutions with participants in Oceanography, Genomics, and Geophysical Sciences. The proposed activity will support the beginning professional career of a female researcher and will serve as the basis for several undergraduate student laboratory projects.
This award supports a project to use two new scanning fluorimeters to map microbial concentrations vs depth in the WAIS Divide ice core as portions of it become available at NICL, and selected portions of the GISP2 ice core for inter-hemispheric comparison. Ground-truth calibrations with microbes in ice show that the instruments are sensitive to a single cell and can scan the full length of a 1-meter core at 300-micron intervals in two minutes. The goals of these studies will be to exploit the discovery that microbes are transported onto ice, in clumps, several times per year and that at rare intervals (not periodically) of ~104 years, a much higher flux, sometimes lasting >1 decade, reaches the ice. From variations ranging from seasonal to millennial to glacial scale in the arrival time distribution of phototrophs, methanogens, and total microbes in the Antarctic and Arctic ice, the investigators will attempt to determine oceanic and terrestrial sources of these microbes and will look for correlations of microbial bursts with dust concentration and temperature proxies. In addition the project will follow up on the discovery that the rare instances of very high microbial flux account for some of the"gas artifacts" in ice cores - isolated spikes of excess CH4 and N2O that have been discarded by others in previous climate studies. The intellectual merit of this project is that it will exploit scanning fluorimetry of microbes as a powerful new tool for studies ranging from meteorology to climatology to biology, especially when combined with mapping of dust, gases, and major element chemistry in ice cores. In 2010-11 the WAIS Divide borehole will be logged with the latest version of the dust logger. The log will provide mm-scale depth resolution of dust concentration and of volcanic ash layers down the entire depth of the borehole. The locations of ash layers in the ice will be determined and chemical analyses of the ash will be analyzed in order to determine provenance. By comparing data from the WAIS Divide borehole with data from other boreholes and with chemical data (obtained by others) on volcanic layers, the researchers will examine the relationship between the timing of volcanic eruptions and abrupt climate change. Results from this project with the scanning fluorimeters and the dust logger could have applications to planetary missions, borehole oceanography, limnology, meteorology, climate, volcanology, and ancient life in ice. A deeper understanding of the causes of abrupt climate change, including a causal relationship with volcanic explosivity, would enable a better understanding of the adverse effects on climate. The broader impact of the project is that it will provide training to students and post-docs from the U. S. and other countries.
Areas of the Southern Ocean have spectacular blooms of phytoplankton during the austral spring and early summer. One of the dominant phytoplankton species, the haptophyte Phaeocystis antarctica, is a prolific producer of the organic sulfur compound dimethylsulfoniopropionate (DMSP) and Phaeocystis blooms are associated with some of the world's highest concentrations of DMSP and its volatile degradation product, dimethylsulfide (DMS). Sulfur, in the form of DMS, is transferred from the oceans to the atmosphere and can affect the chemistry of precipitation and influence cloud properties and possibly climate. DMSP and DMS are also quantitatively significant components of the carbon, sulfur and energy flows in many marine food webs, although very little information is available on these processes in high latitude systems. <br/><br/>This project will study how solar radiation and iron cycling affect DMSP and DMS production by phytoplankton, and the subsequent utilization of these labile forms of organic matter by the microbial food web. Four interrelated hypotheses will be tested in field-based experiments and in situ observations: 1) solar radiation, including enhanced UV-B due to seasonal ozone depletion, plays an important role in determining the net ecosystem production of DMS in the Ross Sea; 2) development of shallow mixed layers promotes the accumulation of DMS in surface waters, because of enhanced exposure of plankton communities to high doses of solar radiation; 3) DMSP production and turnover represent a significant part of the carbon and sulfur flux through polar food webs; 4) bloom development and resulting nutrient depletion (e.g., iron) will result in high production rates of DMSP and high DMS concentrations and atmospheric fluxes. Results from this study will greatly improve understanding of the underlying mechanisms controlling DMSP and DMS concentrations in polar waters, thereby improving our ability to predict DMS fluxes to the atmosphere from this important climatic region. <br/><br/>Both Drs. Kieber and Kiene actively engage high school, undergraduate and graduate students in their research and are involved in formal programs that target underrepresented groups (NSF-REU and the American Chemical Society-SEED). This project will continue this type of educational outreach. The PIs also teach undergraduate and graduate courses and incorporation of research experiences into their classes will enrich student learning experiences.
Areas of the Southern Ocean have spectacular blooms of phytoplankton during the austral spring and early summer. One of the dominant phytoplankton species, the haptophyte Phaeocystis antarctica, is a prolific producer of the organic sulfur compound dimethylsulfoniopropionate (DMSP) and Phaeocystis blooms are associated with some of the world's highest concentrations of DMSP and its volatile degradation product, dimethylsulfide (DMS). Sulfur, in the form of DMS, is transferred from the oceans to the atmosphere and can affect the chemistry of precipitation and influence cloud properties and possibly climate. DMSP and DMS are also quantitatively significant components of the carbon, sulfur and energy flows in many marine food webs, although very little information is available on these processes in high latitude systems. <br/><br/>This project will study how solar radiation and iron cycling affect DMSP and DMS production by phytoplankton, and the subsequent utilization of these labile forms of organic matter by the microbial food web. Four interrelated hypotheses will be tested in field-based experiments and in situ observations: 1) solar radiation, including enhanced UV-B due to seasonal ozone depletion, plays an important role in determining the net ecosystem production of DMS in the Ross Sea; 2) development of shallow mixed layers promotes the accumulation of DMS in surface waters, because of enhanced exposure of plankton communities to high doses of solar radiation; 3) DMSP production and turnover represent a significant part of the carbon and sulfur flux through polar food webs; 4) bloom development and resulting nutrient depletion (e.g., iron) will result in high production rates of DMSP and high DMS concentrations and atmospheric fluxes. Results from this study will greatly improve understanding of the underlying mechanisms controlling DMSP and DMS concentrations in polar waters, thereby improving our ability to predict DMS fluxes to the atmosphere from this important climatic region. <br/><br/>Both Drs. Kieber and Kiene actively engage high school, undergraduate and graduate students in their research and are involved in formal programs that target underrepresented groups (NSF-REU and the American Chemical Society-SEED). This project will continue this type of educational outreach. The PIs also teach undergraduate and graduate courses and incorporation of research experiences into their classes will enrich student learning experiences.
Convincing evidence now confirms that polar regions are changing rapidly in response to human activities. Changes in sea ice extent and thickness will have profound implications for productivity, food webs and carbon fluxes at high latitudes, since sea ice biota are a significant source of biogenic matter for the ecosystem. While sea ice is often thought to be a barrier to gas exchange between the ocean and the atmosphere, it more likely functions as a source or sink for climate-active gases such as carbon dioxide and ozone-depleting organohalogens, due in part to activities of microbes embedded in the sea ice matrix. This project brings together experienced US and Swedish investigators to examine the controls by sea-ice biota on the production and degradation of key climate-active gases in the Pacific sector of the Southern Ocean. We hypothesize that 1) the physical properties of the sea-ice environment will determine the community structure and activities of the sea ice biota; 2) the productivity, biomass, physiological state and species composition of ice algae will determine the production of specific classes of organic carbon, including organohalogens; 3) heterotrophic co-metabolism within the ice will break down these compounds to some extent, depending on the microbial community structure and productivity, and 4) the sea ice to atmosphere fluxes of CO2 and organohalogens will be inversely related. This project will build close scientific collaborations between US and Swedish researchers and also train young scientists, including members of underrepresented groups. Dissemination of results will include the scientific literature, and public outreach venues including interactions with a PolarTrec teacher.
Atmospheric warming has been associated with retreating glaciers, disintegrating ice shelves, and the increasing prevalence of icebergs in the Southern Ocean over the last decade. Our preliminary study of two icebergs in the NW Weddell Sea, an area of high iceberg concentration, showed significant delivery of terrestrial material accompanied by significant enhancement of phytoplankton and zooplankton/micronekton abundance, and primary production surrounding the icebergs. We hypothesize that nutrient enrichment by free-drifting icebergs will increase primary production and sedimentation of organic carbon, thus increasing the draw-down and sequestration of CO2 in the Southern Ocean and impacting the global carbon cycle. Our research addresses the following questions:1) What is the relationship between the physical dynamics of free-drifting icebergs and the Fe and nutrient distributions of the surrounding water column? 2) What is the relationship between Fe and nutrient distributions associated with free-drifting icebergs and the organic carbon dynamics of the ice-attached and surrounding pelagic communities (microbes, zooplankton, micronekton)? 3) What is impact on the export flux of particulate organic carbon from the mixed layer? An interdisciplinary approach is proposed to examine iceberg structure and dynamics, biogeochemical processes, and carbon cycling that includes measurement of trace element, nutrient and radionuclide distributions; organic carbon dynamics mediated by microbial, ice-attached and zooplankton communities; and particulate organic carbon export fluxes. Results from this project will further our understanding of the relationship between climate change and carbon sequestration in the Southern Ocean. Our findings will be incorporated into the Antarctic Research division of the Ocean Exploration Center (OEC) as part of the SIOExplorer: Digital Library Project. The OEC allows users to access content, which is classified to one of four levels: entry (grade K-6), student (grade 6-12), college, and research. Graduate students, undergraduates, teachers, and volunteers are important participants in the proposed field and laboratory work. For the K-12 level, a professional writer of children's books will participate in cruises to produce an account of the expedition and a daily interactive website.
Atmospheric warming has been associated with retreating glaciers, disintegrating ice shelves, and the increasing prevalence of icebergs in the Southern Ocean over the last decade. Our preliminary study of two icebergs in the NW Weddell Sea, an area of high iceberg concentration, showed significant delivery of terrestrial material accompanied by significant enhancement of phytoplankton and zooplankton/micronekton abundance, and primary production surrounding the icebergs. We hypothesize that nutrient enrichment by free-drifting icebergs will increase primary production and sedimentation of organic carbon, thus increasing the draw-down and sequestration of CO2 in the Southern Ocean and impacting the global carbon cycle. Our research addresses the following questions:1) What is the relationship between the physical dynamics of free-drifting icebergs and the Fe and nutrient distributions of the surrounding water column? 2) What is the relationship between Fe and nutrient distributions associated with free-drifting icebergs and the organic carbon dynamics of the ice-attached and surrounding pelagic communities (microbes, zooplankton, micronekton)? 3) What is impact on the export flux of particulate organic carbon from the mixed layer? An interdisciplinary approach is proposed to examine iceberg structure and dynamics, biogeochemical processes, and carbon cycling that includes measurement of trace element, nutrient and radionuclide distributions; organic carbon dynamics mediated by microbial, ice-attached and zooplankton communities; and particulate organic carbon export fluxes. Results from this project will further our understanding of the relationship between climate change and carbon sequestration in the Southern Ocean. Our findings will be incorporated into the Antarctic Research division of the Ocean Exploration Center (OEC) as part of the SIOExplorer: Digital Library Project. The OEC allows users to access content, which is classified to one of four levels: entry (grade K-6), student (grade 6-12), college, and research. Graduate students, undergraduates, teachers, and volunteers are important participants in the proposed field and laboratory work. For the K-12 level, a professional writer of children's books will participate in cruises to produce an account of the expedition and a daily interactive website.
Data collected on the permanently ice-covered lakes of the McMurdo Dry Valleys (MCM) during the late 1950's as part of the International Geophysical Year (IGY) showed that they were the only year round liquid water environments on the continent. Organisms in the lakes must possess novel physiological strategies that allow them to survive at low temperature and under extended darkness. Subsequent research has now shown that most organisms in the lakes are not just "surviving the extremes" but are actively feeding, growing and reproducing. However, nearly all research on the MCM lakes is restricted to the austral spring and summer when logistical support is provided. The unique aspects of physiological adaptation and metabolic function during the permanently cold and prolonged darkness of the Antarctic winter remain unknown. As part of the "International Polar Year 2007-2008" (IPY), the proposed research will study lakes within the Taylor Valley during the transition to polar night to test the overarching hypothesis that the onset of darkness induces a cascade of physiological changes that alters the functional role of autotrophic and heterotrophic microplankton within the lakes. This overarching theme will be addressed through an interdisciplinary study of selected biological components of the lake ecosystems using genomic and physiological tools to understand not only how individual organisms survive, but how they control ecosystem function during this seasonal transition. <br/><br/>This project is directly relevant to IPY objectives as it addresses a major identified theme (Adaptations to Life in Extreme Cold and Prolonged Darkness) with an international (UK, NZ),<br/>multidisciplinary team. The research has substantial broader impacts, as it will add to the body of long-term data accumulated by the MCM LTER and MCM Microbial Observatory projects in a synergistic manner; and it will include three undergraduates, a graduate student and two young female investigators. The project is linked to a highly visible education, outreach and human diversity programs supported by the McMurdo LTER, and initiates new outreach programs, including the Passport to Knowledge program.
Fungi in Antarctic ecosystems are major contributors to biodiversity and have great influence on many processes such as biodegradation and nutrient cycling. It is essential for biological surveys as well as genomic and proteomic studies to be completed so a better understanding of these organisms is obtained. Previous research has identified unique fungi associated with historic wooden structures brought to Antarctica by Robert F. Scott and Ernest Shackleton during the Heroic Era of exploration. Many of the fungi found are previously undescribed species that belong to the little known genus Cadophora. The research team will obtain important new information on the fungi present in the Ross Sea and Peninsula Regions of Antarctica, particularly their role in decomposition and nutrient recycling and their mechanisms and strategies for survival in the polar environment. New tools and methods include denaturing gradient gel electrophoresis (DGGE), real-time PCR, and proteomic profiling. These analyses will reveal key details of the physiological adaptations these fungi have evolved to carry out processes such as biodegradation and nutrient cycling under conditions that would inhibit other fungi. This work, coupled with the training and learning opportunities it provides, will be of value to many fields of study including microbial ecology, polar biology, wood microbiology, environmental science, soil science, geobiochemistry, and mycology as well as fungal phylogenetics, proteomics and genomics. Results obtained will have immediate applied use to help preserve and protect Antarctica's historic monuments. The investigations proposed are a continuation of research to identify the microbes attacking these historic structures and artifacts and to elucidate their biology and ecology in the polar environment. New research will also be done at the historic Cape Adare huts, the first wooden structures to be built in Antarctica and also at East Base, an American historic site on Stonington Island from the Admiral Byrd and Ronne Expeditions of 1939-1948. The research team will conduct vital studies needed to successfully conserve the wooden structures and artifacts at these sites and protect them for future generations
The Antarctic Peninsula region exhibits one of the largest warming trends in the world. Climate change in this region will reduce the duration of winter sea-ice cover, altering both the pelagic ecosystem and bentho-pelagic coupling. We postulate that shelf benthic ecosystems are highly suitable for tracking climate change because they act as "low-pass" filters, removing high-frequency seasonal noise and responding to longer-term trends in pelagic ecosystem structure and export production. We propose to conduct a 3-year study of bentho-pelagic coupling along a latitudinal climate gradient on the Antarctic Peninsula to explore the potential impacts of climate change (e.g., reduction in sea-ice duration) on Antarctic shelf ecosystems. We will conduct three cruises during summer and winter regimes along a 5- station transect from Smith Island to Marguerite Bay, evaluating a broad range of benthic ecological and biogeochemical processes. Specifically, we will examine the feeding strategies of benthic deposit feeders along this climatic gradient to elucidate the potential response of this major trophic group to climatic warming. In addition, we will (1) quantify carbon and nitrogen cycling and burial at the seafloor and (2) document changes in megafaunal, macrofaunal, and microbial community structure along this latitudinal gradient. We expect to develop predictive insights into the response of Antarctic shelf ecosystems to some of the effects of climate warming (e.g., a reduction in winter sea-ice duration). The proposed research will considerably broaden the ecological and carbon-flux measurements made as parts of the Palmer Station LTER and GLOBEC programs by providing a complementary benthic component. This project also will promote science education from the 9th grade to graduate-student levels. We will partner with the NSF-sponsored Southeastern Center for Ocean Science Education Excellence to reach students of all races in all areas of NC, SC and GA. The project will also benefit students at the post secondary level by supporting three graduate and two undergraduate students. During each of the three field excursions, NCSU and UH students will travel to Chile and Antarctica to participate in scientific research. Lastly, all three PIs will incorporate material from this project into their undergraduate and graduate courses.
*** Caron 9714299 The analysis of microbial biodiversity of extreme environments is difficult because traditional methods for examining diversity are often ineffective for assessing species richness within these communities. Additional difficulties arise due to the difficulties of recreating and maintaining pertinent environmental features during sample collection and procession. This study focuses on the protistan assemblages (algae and protozoa) in the sea ice, sediment and ocean environments of the Ross Sea, Antarctica. The identification of protistan species in natural assemblages traditionally has entailed direct microscopical analyses as well as enrichment and culture techniques for assessing biodiversity. Determination of diversity for these assemblages in therefore susceptible to biases as a consequence of sampling, enrichment and culture, as well as selective losses due to sample preservation and concentration for microscopy. The goals of this project are: (1) to develop and apply molecular biological approaches to assess species diversity of small protists (algae and protozoa smaller than 100 micrometers) in ocean water, sea ice and sediment environments and (2) to obtain baseline physiological information on the growth rates, feeding rates and growth efficiencies of cultured protozoa under pertinent temperature regimes. Molecular biological studies will involve the use of PCR-based protocols to examine small subunit ribosomal RNA gene (srDNA) diversity. Approaches and techniques developed will be applicable to any other water body or sediment and would provide a means to examine the representativeness of protistan cultures in extant culture collections. ***
The U.S. Global Ocean Ecosystems Dynamics (U.S. GLOBEC) program has the goal of understanding and ultimately predicting how populations of marine animal species respond to natural and anthropogenic changes in climate. Research in the Southern Ocean (SO) indicates strong coupling between climatic processes and ecosystem dynamics via the annual formation and destruction of sea ice. The Southern Ocean GLOBEC Program (SO GLOBEC) will investigate the dynamic relationship between physical processes and ecosystem responses through identification of critical parameters that affect the distribution, abundance and population dynamics of target species. The overall goals of the SO GLOBEC program are to elucidate shelf circulation processes and their effect on sea ice formation and krill distribution, and to examine the factors which govern krill survivorship and availability to higher trophic levels, including penguins, seals and whales. The focus of the U.S. contribution to the international SO GLOBEC program will be on winter processes. This component will focus on the distribution and activities of sea ice microbial communities. This will be accomplished using an integrated combination of sampling (vertical profiles, horizontal surveys, and under-ice surveys) and observational protocols. Experiments will be designed to estimate microbial activity within the sea ice and at the ice-seawater interface. The research will be coordinated with components studying the water column productivity and the sea ice habitat. The result of the integrated SO GLOBEC program will be to improve the predictability of living marine resources, especially with respect to local and global climatic shifts.
This project brings together researchers with expertise in molecular microbial ecology, Antarctic and deep sea environments, and metagenomics to address the overarching question: how do ecosystems dominated by microorganisms adapt to conditions of continuous cold and dark over evolutionarily and geologically relevant time scales? Lake Vostok, buried for at least 15 million years beneath approximately 4 km of ice that has prevented any communication with the external environment for as much as 1.5 million years, is an ideal system to study this question. Water from the lake that has frozen on to the bottom of the ice sheet (accretion ice) is available for study. Several studies have indicated the presence of low abundance, but detectable microbial communities in the accretion ice. Our central hypothesis maintains that Lake Vostok microbes are specifically adapted to life in conditions of extreme cold, dark, and oligotrophy and that signatures of those adaptations can be observed in their genome sequences at the gene, organism, and community levels. To address this hypothesis, we propose to characterize the metagenome (i.e. the genomes of all members of the community) of the accretion ice. using whole genome amplification (WGA), which can provide micrograms of unbiased metagenomic DNA from only a few cells. The results of this project have relevance to evolutionary biology and ecology, subglacial Antarctic lake exploration, biotechnology, and astrobiology. The project directly addresses priorities and themes in the International Polar Year at the national and international levels. A legacy of DNA sequence data and the metagenomic library will be created and maintained. Press releases and a publicly available web page will facilitate communication with the public. K-12 outreach will be the focus of a new, two-tiered program targeting the 7th grade classroom and on site visits to the Joint Genome Institute Production Sequencing Facility by high school juniors and seniors and community college level students. Minority undergraduate researchers will be recruited for research on this project, and support and training are provided to two graduate students, a postdoctoral scholar, and a technician.
This project studies the microbial processes that alter volcanic glass, which is critical to understanding the earliest life on earth. To understand the environmental controls on these processes, this project uses the extreme environments of the McMurdo region of Antarctica as a natural laboratory. Volcanic glass substrates are placed in hydrothermal systems, lakes, and other areas for two to four years to identify colonizing microbial consortia and the chemical processes of microbe-glass interaction. Recovered experiments are analyzed to explore the role of eukaryotic and prokaryotic organisms, and the relevance of autotrophs during colonization and biofilm formation using microscopic, molecular and culture techniques. <br/><br/>The broader impacts include graduate and undergraduate student participation in research and K-12 outreach and teacher training.
Dissolved organic matter (DOM) is a significant chemical component in aquatic systems because it acts as an important carbon source for microorganisms, absorbs harmful radiation in sunlight, is able to complex metals, and can participate in important biogeochemical reactions. This study will investigate the biogeochemical cycling of DOM in a small coastal Antarctic pond, Pony Lake, located on Cape Royds, Ross Island. Because there are no higher plants present at this site all of the DOM in this lake is derived from microorganisms. Thus, Pony Lake is an ideal site to study the effect of physical, chemical, and microbial processes on the composition and character of the DOM pool. Finally, Pony Lake is also an ideal site to collect an International Humic Substances Society (IHSS) fulvic acid standard. Unlike other IHSS standards, this standard will not contain DOM components derived from higher land plants. To better understand the role of physical influences, the project will study the changes in the DOM pool as the lake evolves from ice-covered to ice-free conditions during the summer, as well as the relationship of DOM to the observed turnover of dominant microbial communities in the lake. Scientists will also monitor changes in microbial abundance, diversity, and productivity that may occur during the ice to open-water transition period. This research will provide much needed information regarding the relationship between microbial diversity and DOM biogeochemistry. Middle school science students will be active participants in this project through the Internet, while scientists are in the field, and in the lab.
Abstract<br/><br/>The research will continue and extend the study in the Southern Ocean that was initiated during the Oden Southern Ocean 2006 expedition in collaboration with Swedish scientist Mellissa Chierici. We will quantify carbon flux through the food web in the marginal ice zone (MIZ) by measuring size fractionated primary and secondary production, grazing and carbon flux through nanoplankton (2-20 um), microplankton (20-200um), and mesoplankton (200-2000 um). Community structure, species abundance and size specific grazing rates will be quantified using a variety of techniques both underway and at ice stations along the MIZ. The proposed cruise track extends across the Drake Passage to the Western Antarctic Peninsula (WAP) with three station transects along a gradient from the open ocean through the marginal ice zone (MIZ) in the Bellinghausen and Amundsen Seas and into the Ross Sea Polynya. Ice stations along each transect will provide material to characterize production associated with annual ice. Underway measurements of primary and secondary production (chlorophyll, CDOM, microplankton, and mesoplankton) and hydrography (temperature, salinity, pH, DO, turbidity) will establish a baseline for future cruises and as support for other projects such as biogeochemical studies on carbon dioxide drawdown and trace metal work on primary production. The outcome of these measurements will be a description of nano to mesoplankton standing stocks, community structure, and carbon flux along the MIZ in the Bellinghausen and Amundsen Seas and the Ross Sea Polynya.
Denitrification is the main process by which fixed nitrogen is lost from ecosystems and the regulation of this process may directly affect primary production and carbon cycling over short and long time scales. Previous investigations of the role of bioactive metals in regulating denitrification in bacteria from permanently ice-covered Lake Bonney in the Taylor Valley of East Antarctica indicated that denitrifying bacteria can be negatively affected by metals such as copper, iron, cadmium, lead, chromium, nickel, silver and zinc; and that there is a distinct difference in denitrifying activity between the east and west lobes of the lake. Low iron concentrations were found to exacerbate the potential toxicity of the other metals, while silver has the potential to specifically inhibit denitrification because of its ability to interfere with copper binding in redox proteins, such as nitrite reductase and nitrous oxide reductase. High silver concentrations might prevent the functioning of nitrous oxide reductase in the same way that simple copper limitation does, thereby causing the buildup of nitrous oxide and resulting in a nonfunctional nitrogen cycle. Other factors, such as oxygen concentration, are likely also to affect bacterial activity in Lake Bonney. This project will investigate silver toxicity, general metal toxicity and oxygen concentration to determine their effect on denitrification in the lake by using a suite of "sentinel" strains of denitrifying bacteria (isolated from the lake) incubated in Lake Bonney water and subjected to various treatments. The physiological responses of these strains to changes in metal and oxygen concentration will be quantified by flow cytometric detection of single cell molecular probes whose sensitivity and interpretation have been optimized for the sentinel strains. Understanding the relationships between metals and denitrification is expected to enhance our understanding of not only Lake Bonney's unusual nitrogen cycle, but more generally, of the potential role of metals in the regulation of microbial nitrogen transformations.<br/><br/>The broader impacts of this work include not only a better understanding of regional biogeochemistry and global perspectives on these processes; but also the training of graduate students and a substantial outreach effort for school children.
Aquatic-terrestrial transition zones are crucial environments in understanding the biogeochemistry of landscapes. In temperate watersheds, these areas are generally dominated by riparian zones, which have been identified as regions of special interest for biogeochemistry because of the increased microbial activity in these locations, and because of the importance of these hydrological margins in facilitating and buffering hydrologic and biogeochemical exchanges between terrestrial and aquatic ecosystems. In the Antarctic Dry Valleys, terrestrial-aquatic transition zones are intriguing landscape features because of the vast importance of water in this polar desert, and because the material and energy budgets of dry valley ecosystems are linked by hydrology. Hydrological margins in aquatic-terrestrial transition zones will be studied in the Dry Valleys of Antarctica to answer two overarching questions: (1) what are the major controls over hydrologic and biogeochemical exchange across aquaticterrestrial transition zones and (2) to what extent do trends in nutrient cycling (e.g. nitrogen cycling) across these transition zones reflect differences in microbial communities or function vs. differences in the physical and chemical environment (e.g., redox potential)? The hydrologic gradients that define these interfaces provide the opportunity to assess the relative influence of physical conditions and microbial biodiversity and functioning upon biogeochemical cycling. Coordinated hydrologic, biogeochemical, and molecular microbial studies will be executed within hydrologic margins with the following research objectives: to determine the role of sediment characteristics, permafrost and active layer dynamics, and topography on sub-surface water content and distribution in hydrologic margins, to determine the extent to which transformations of nitrogen in hydrological margins are influenced by physical conditions (i.e., moisture, redox potential and pH) or by the presence of specific microbial communities (e.g., denitrifiers), and to characterize the microbial community structure and function of saturated zones.<br/><br/>This proposed research will provide an improved understanding of the interaction of liquid water, soils, microbial communities, and biogeochemistry within the important hydrologic margin landscape units of the dry valleys. Dry valleys streams and lakes are unique because there is no influence of higher vegetation on the movement of water and may therefore provide a model system for understanding physical and hydrological influences on microbial ecology and biogeochemistry. Hence the findings will contribute to Antarctic science as well as the broader study of riparian zones and hydrologic margins worldwide. Graduate students and undergraduate students will be involved with fieldwork and research projects. Information will be disseminated through a project web site, and outreach activities will include science education in local elementary, middle and high schools near the three universities involved.
The large subglacial Lake Vostok in Antarctica is unique ecological site with a novel microbial biota. The temperatures, pressures and lack of light all select for organisms that may not exist anywhere else on Earth. The accretion ice (lake water frozen to the bottom of the lower surface of the glacier) has preserved microbial samples from each region of Lake Vostok as the glacier passes over and into the lake. Thus, without contaminating the lake with microorganisms from the surface, microbes originating from the lake can be collected, transported to the laboratory and studied. Two of the deepest ice cores sections in this project are part of the international allocation. The will be shared between four researchers (Sergey Bulat from Russia, Jean-Robert Petit and Daniel Prieur from France, Scott Rogers from USA). The United States team will study, isolate, and characterize bacteria, fungi, and viruses that have been sampled from the lake through the process of ice accretion to the lower surface of 3500+m thick glacier overriding the lake. The project will involve a suite of methods, including molecular, morphological, and cultural. This includes observation and description by fluorescence, light, and electron microscopy, isolation on thirteen separate cultural media, polymerase chain reaction amplification, DNA sequencing, and phylogenetic analyses. Eleven accretion ice core sections, as well as two glacial ice core sections. As well as two glacial ice core sections will be studied. The accretion ice core sections, as well as two glacial ice core sections will be studied. The accretion ice core sections represent all of the major regions of the lake that have been sampled by the accretion process in the vicinity of the Vostok 5G ice core. The broader impacts of the work relate to the impact the results will have on the filed. These long=isolated lakes, deep below the Antarctic ice sheet may contain novel uniquely adapted organisms. Glacial ice contains an enormous diversity of entrapped microbes, some of which may be metabolically active in the ice. The microbes from Lake Vostok are of special interest, since they are adapted to cold, dark, and high pressure. Thus, their enzyme systems and biochemical pathways may be significantly different from those in the microbes that are the subject of current studies. As such, these organisms may form compounds that may have useful applications. Also, study of the accretion ice, and eventually the water, from Lake Vostok will provide a basis for the study of other subglacial lakes. Additionally, study of the microbes in the accretion ice will be useful to those planning to study analogous systems on ice-covered planets and moons.
Ultraviolet radiation influences the dynamics of plankton processes in the near-surface waters of most aquatic ecosystems. In particular, the Southern Ocean is affected in the austral spring period when biologically damaging ultraviolet radiation is enhanced by ozone depletion. While progress has been made in estimating the quantitative impact of ultraviolet radiation on bacteria and phytoplankton in the Southern Ocean, some important issues remain to be resolved. Little is known about responses in systems dominated by the colonial haptophyte Phaeocystis antarctica, which dominates spring blooms in a polyna that develops in the southern Ross Sea. The Ross Sea is also of interest because of the occurrence of open water at a far southerly location in the spring, well within the ozone hole, and continuous daylight, with implications for the regulation of DNA repair. A number of studies suggest that vertical mixing can significant modify the impact of ultraviolet radiation in the Southern Ocean and elsewhere. However, there are limited measurements of turbulence intensity in the surface layer and measurements have not been integrated with parallel studies of ultraviolet radiation effects on phytoplankton and bacterioplankton. To address these issues, this collaborative study will focus on vertical mixing and the impact of ultraviolet radiation in the Ross Sea. The spectral and temporal responses of phytoplankton and bacterioplankton to ultraviolet radiation will be characterized in both laboratory and solar incubations. These will lead to the definition of biological weighting functions and response models capable of predicting the depth and time distribution of ultraviolet radiation impacts on photosynthesis, bacterial incorporation and DNA damage in the surface layer. Diel sampling will measure depth-dependent profiles of DNA damage, bacterial incorporation, photosynthesis and fluorescence parameters over a 24 h cycle. Sampling will include stations with contrasting wind-driven mixing and stratification as the polyna develops. The program of vertical mixing measurements is optimized for the typical springtime Ross Sea situation in which turbulence of intermediate intensity is insufficient to mix the upper layer thoroughly in the presence of stabilizing influences like solar heating and/or surface freshwater input from melting ice. Fine-scale vertical density profiles will be measured with a free-fall CTD unit and the profiles will be used to directly estimate large-eddy scales by determining Thorpe scales. Eddy scales and estimated turbulent diffusivities will be directly related to surface layer effects, and used to generate lagrangian depth-time trajectories in models of ultraviolet radiation responses in the surface mixed layer. The proposed research will be the first in-depth study of ultraviolet radiation effects in the Ross Sea and provide a valuable comparison with previous work in the Weddell-Scotia Confluence and Palmer Station regions. It will also enhance the understanding of vertical mixing processes, trophic interactions and biogeochemical cycling in the Ross Sea.
This award supports a project to use three downhole instruments - an optical logger; a<br/>miniaturized biospectral logger at 420 nm (miniBSL-420); and an Acoustic TeleViewer (ATV) - to log a 350-m borehole at the WAIS Divide drill site. In addition, miniBSL-224 (at 224 nm) and miniBSL-420 will scan ice core sections at NICL to look for abrupt climate changes, volcanic ash, microbial concentrations, and correlations among them. Using the optical logger and ATV to log bubble number densities vs depth in a WAIS Divide borehole, we will detect annual layers, from which we can establish the age vs depth relation to the bottom of the borehole that will be available during the three-year grant period. With the same instruments we will search for long-period modulation of bubble and dust concentrations in order to provide definitive evidence for or against an effect of long-period variability of the sun or solar wind on climate. We will detect and accurately date ash layers in a WAIS Divide borehole. We will match them with ash layers that we previously detected in the Siple Dome borehole, and also match them with sulfate and ash layers found by others at Vostok, Dome Fuji, Dome C, and GISP2. The expected new data will allow us to extend our recent study which showed that the Antarctic record of volcanism correlates with abrupt climate change at a 95% to >99.8% significance level and that the volcanic signatures at bipolar locations match at better than 3 sigma during the interval 2 to 45 kiloyears. The results to be obtained during this grant period will position us to extend an accurate age vs depth relation and volcano-climate correlations to earlier than 150 kiloyears ago in the future WAIS Divide borehole to be drilled to bedrock. Using the miniBSLs to identify biomolecules via their fluorescence, we will log a 350-m borehole at WAIS Divide, and we will scan selected lengths of ice core at NICL. Among the biomolecules the miniBSLs can identify will be chlorophyll, which will provide the first map of aerobic microbes in ice, and F420, which will provide the first map of methanogens in ice. We will collaborate with others in relating results from WAIS Divide and NICL ice cores to broader topics in climatology, volcanology, and microbial ecology. We will continue to give broad training to undergraduate and graduate students, to attract underrepresented minorities to science, engineering, and math, and to educate the press and college teachers. A deeper understanding of the causes of abrupt climate change, including a causal relationship with strong volcanic eruptions, can enable us to understand and mitigate adverse effects on climate.