USAP-DC

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{"@context": "https://schema.org/", "@type": "Dataset", "@id": "https://www.usap-dc.org/view/dataset/601982", "additionalType": ["geolink:Dataset", "vivo:Dataset"], "name": "De novo assembled contigs for a cryoconite microcosm study - Canada Glacier Cryoconite hole sample - NCBI BioProject PRJNA1315162", "description": "This dataset has short read sequences from of communities exposed to nutrient enrichment under microcosm conditions. The original communities came from cryoconite hole sediments from Canada Glacier, Taylor Valley, Antarctica. Homogenized sediments were then distributed among 20 sterile Petri dishes (60 mm), each with 25 g dry weight equivalent (31.25 g wet sediment based on 25% water content) and assigned to five experimental treatments. Treatments consisted of 1) Bulk, sediments that received neither water nor nutrients, 2) Control, sediments that received additions of 1 ml of water, 3) +N, sediments that received 1 ml of ammonium nitrate solution to achieve 4 \u00b5mol N * g soil-1, 3) +P, sediments that received 1 ml of sodium phosphate dibasic solution to achieve 2 \u00b5mol P * g soil-1, and 5) +NP, sediments that received 1 ml of combined ammonium nitrate and sodium phosphate dibasic to achieve 4 \u00b5mol N + 2\u00b5mol P * g soil-1. Each treatment was replicated five times and incubated in a freeze-thaw growth chamber with temperatures cycling between 1\u00b0C and 3\u00b0C and light between ~100 Lux (5 hrs/day) and ~10,000 Lux (19 hrs per day) to imitate natural cryoconite holes conditions during austral summers.", "citation": "Varsani, A. (2025) \"De novo assembled contigs for a cryoconite microcosm study - Canada Glacier Cryoconite hole sample - NCBI BioProject PRJNA1315162\" U.S. Antarctic Program (USAP) Data Center. doi: https://doi.org/None.", "datePublished": "2025-09-05", "keywords": ["Antarctica", "Cryosphere", "Taylor Valley"], "creator": [{"@type": "Person", "additionalType": "geolink:Person", "name": "Varsani, Arvind", "email": "Arvind.Varsani@asu.edu", "affiliation": {"@type": "Organization", "name": null}}], "distribution": [{"@type": "DataDownload", "additionalType": "http://www.w3.org/ns/dcat#DataCatalog", "encodingFormat": "text/xml", "name": "ISO Metadata Document", "url": "https://www.usap-dc.org/metadata/isoxml/601982iso.xml", "contentUrl": "/dataset/filename"}, {"@type": "DataDownload", "@id": "https://www.usap-dc.org/view/dataset/601982", "additionalType": "dcat:distribution", "url": "https://www.usap-dc.org/view/dataset/601982", "contentUrl": "/dataset/filename", "encodingFormat": "text/html"}, {"@type": "DataDownload", "@id": "https://www.usap-dc.org/view/dataset/601982", "additionalType": "dcat:distribution", "url": "https://www.usap-dc.org/view/dataset/601982", "contentUrl": "/dataset/filename", "encodingFormat": "text/html"}], "identifier": {"@type": "PropertyValue", "url": "https://www.usap-dc.org/view/dataset/601982", "value": "https://www.usap-dc.org/view/dataset/601982"}, "contributor": [{"@type": "Role", "roleName": "credit", "description": "funderName:NSF:GEO:OPP:Antarctic Organisms and Ecosystems awardNumber:2137378 awardTitle:Collaborative Research: Role of Nutrient Limitation and Viral Interactions on Antarctic Microbial Community Assembly: A Cryoconite Microcosm Study"}], "license": [{"@type": "CreativeWork", "URL": "https://creativecommons.org/licenses/by/4.0/", "name": "MD_Constraints", "description": "useLimitation: Creative Commons Attribution Only v4.0 Generic [CC BY 4.0]."}, {"@type": "CreativeWork", "name": "MD_LegalConstraints", "description": "accessConstraints: license. otherConstraints: Creative Commons Attribution Only v4.0 Generic [CC BY 4.0]."}, {"@type": "CreativeWork", "name": "MD_SecurityConstraints", "description": "classification: "}], "publisher": {"@type": "Organization", "name": "U.S. Antarctic Program (USAP) Data Center"}, "spatialCoverage": [{"@type": "Place", "geo": {"@type": "GeoShape", "box": "-77.5699 162.2955 -77.5699 162.2955"}}]}

De novo assembled contigs for a cryoconite microcosm study - Canada Glacier Cryoconite hole sample - NCBI BioProject PRJNA1315162 Cite as Varsani, A. (2025) "De novo assembled contigs for a cryoconite microcosm study - Canada Glacier Cryoconite hole sample - NCBI BioProject PRJNA1315162" U.S. Antarctic Program (USAP) Data Center. doi: https://doi.org/None. Abstract This dataset has short read sequences from of communities exposed to nutrient enrichment under microcosm conditions. The original communities came from cryoconite hole sediments from Canada Glacier, Taylor Valley, Antarctica. Homogenized sediments were then distributed among 20 sterile Petri dishes (60 mm), each with 25 g dry weight equivalent (31.25 g wet sediment based on 25% water content) and assigned to five experimental treatments. Treatments consisted of 1) Bulk, sediments that received neither water nor nutrients, 2) Control, sediments that received additions of 1 ml of water, 3) +N, sediments that received 1 ml of ammonium nitrate solution to achieve 4 µmol N * g soil-1, 3) +P, sediments that received 1 ml of sodium phosphate dibasic solution to achieve 2 µmol P * g soil-1, and 5) +NP, sediments that received 1 ml of combined ammonium nitrate and sodium phosphate dibasic to achieve 4 µmol N + 2µmol P * g soil-1. Each treatment was replicated five times and incubated in a freeze-thaw growth chamber with temperatures cycling between 1°C and 3°C and light between ~100 Lux (5 hrs/day) and ~10,000 Lux (19 hrs per day) to imitate natural cryoconite holes conditions during austral summers. Creator(s): Varsani, Arvind Date Created: 2025-09-10 Repository: USAP-DC (current) License: Creative Commons Attribution Only v4.0 Generic [CC BY 4.0] Spatial Extent(s) West: 162.2955, East: 162.2955, South: -77.5699, North: -77.5699 Award(s) NSF 2137378 Version: 1 Related Project(s) Collaborative Research: Role of Nutrient Limitation and Viral Interactions on Antarctic Microbial Community Assembly: A Cryoconite Microcosm Study Keywords Antarctica Cryosphere Taylor Valley