USAP-DC

[{"dataset_id": "601089", "west": 161.1667, "east": 161.667, "south": -77.117, "north": -77.117, "gid": 672, "geometry": "0101000020E6100000CEAACFD5562D6440736891ED7C4753C0", "cross_dateline": false, "bounds_geometry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}]

{"@context": "https://schema.org/", "@type": "Dataset", "@id": "doi:10.15784/601089", "additionalType": ["geolink:Dataset", "vivo:Dataset"], "name": "UPLC-Q-TOF data of Cotton Glacier exometabolites", "description": "Mass spectra of external metabolites were obtained with a 1290 Ultra Performance Liquid Chromatography system coupled to a 6538 Ultra High Definition Accurate-Mass Quadrupole-Time of Flight mass spectrometer operated in positive mode with an electrospray ionization source (Agilent Technologies). 30 mL of filtered media was concentrated per sample by solid phase extraction. External metabolites were re-suspended in 50% (v/v) acetonitrile, and were separated using a reverse-phase Kinetix 1.7 um C18, 100A, 150 mm - 2.1 mm column. Data presented are from UPLC-Q-TOF measurements of mass to charge ratio, retention time, and replicate-averaged extracted ion chromatogram abundance values (counts) of molecular species that demonstrated a significant change in abundance (Two-way ANOVA, adjusted P<0.01) during incubations based on time point (T0: d0, T1: d27, T2: d63, T3: d98) and carbon source (Cotton Glacier: CG, Pony Lake: PL, Suwannee River: SR).", "citation": "Tigges, M., Bothner, B., & Foreman, C. (2018) \"UPLC-Q-TOF data of Cotton Glacier exometabolites\" U.S. Antarctic Program (USAP) Data Center. doi: https://doi.org/10.15784/601089.", "datePublished": "2018-03-23", "keywords": ["Antarctica", "Biota", "Biota", "Exometabolites", "Mass Spectrometry", "Microbes", "Microbiology"], "creator": [{"@type": "Person", "additionalType": "geolink:Person", "name": "Bothner, Brian", "email": null, "affiliation": {"@type": "Organization", "name": null}}, {"@type": "Person", "additionalType": "geolink:Person", "name": "Foreman, Christine", "email": "cforeman@montana.edu", "affiliation": {"@type": "Organization", "name": "Montana State University"}}, {"@type": "Person", "additionalType": "geolink:Person", "name": "Tigges, Michelle", "email": null, "affiliation": {"@type": "Organization", "name": null}}], "distribution": [{"@type": "DataDownload", "additionalType": "http://www.w3.org/ns/dcat#DataCatalog", "encodingFormat": "text/xml", "name": "ISO Metadata Document", "url": "https://www.usap-dc.org/metadata/isoxml/601089iso.xml", "contentUrl": "/dataset/filename"}, {"@type": "DataDownload", "@id": "http://dx.doi.org/10.15784/601089", "additionalType": "dcat:distribution", "url": "http://dx.doi.org/10.15784/601089", "contentUrl": "/dataset/filename", "encodingFormat": "text/html"}, {"@type": "DataDownload", "@id": "https://www.usap-dc.org/view/dataset/601089", "additionalType": "dcat:distribution", "url": "https://www.usap-dc.org/view/dataset/601089", "contentUrl": "/dataset/filename", "encodingFormat": "text/html"}], "identifier": {"@type": "PropertyValue", "propertyID": "https://registry.identifiers.org/registry/doi", "value": "doi:10.15784/601089", "url": "https://doi.org/10.15784/601089"}, "contributor": [{"@type": "Role", "roleName": "credit", "description": "funderName:NSF:GEO:PLR:Antarctic Organisms and Ecosystems awardNumber:1141978 awardTitle:Multidimensional \"omics\" characterization of microbial metabolism and dissolved organic matter in Antarctica"}], "license": [{"@type": "CreativeWork", "URL": "https://creativecommons.org/licenses/by-nc/4.0/", "name": "MD_Constraints", "description": "useLimitation: Creative Commons Attribution-NonCommercial v4.0 Generic [CC BY-NC 4.0]."}, {"@type": "CreativeWork", "name": "MD_LegalConstraints", "description": "accessConstraints: license. otherConstraints: Creative Commons Attribution-NonCommercial v4.0 Generic [CC BY-NC 4.0]."}, {"@type": "CreativeWork", "name": "MD_SecurityConstraints", "description": "classification: "}], "publisher": {"@type": "Organization", "name": "U.S. Antarctic Program (USAP) Data Center"}, "spatialCoverage": [{"@type": "Place", "geo": {"@type": "GeoShape", "box": "-77.117, 161.1667, -77.117, 161.667"}}]}

UPLC-Q-TOF data of Cotton Glacier exometabolites Data DOI: https://doi.org/10.15784/601089 Cite as Tigges, M., Bothner, B., & Foreman, C. (2018) "UPLC-Q-TOF data of Cotton Glacier exometabolites" U.S. Antarctic Program (USAP) Data Center. doi: https://doi.org/10.15784/601089. AMD - DIF Record(s) USAP-1141978 Abstract Mass spectra of external metabolites were obtained with a 1290 Ultra Performance Liquid Chromatography system coupled to a 6538 Ultra High Definition Accurate-Mass Quadrupole-Time of Flight mass spectrometer operated in positive mode with an electrospray ionization source (Agilent Technologies). 30 mL of filtered media was concentrated per sample by solid phase extraction. External metabolites were re-suspended in 50% (v/v) acetonitrile, and were separated using a reverse-phase Kinetix 1.7 um C18, 100A, 150 mm - 2.1 mm column. Data presented are from UPLC-Q-TOF measurements of mass to charge ratio, retention time, and replicate-averaged extracted ion chromatogram abundance values (counts) of molecular species that demonstrated a significant change in abundance (Two-way ANOVA, adjusted P<0.01) during incubations based on time point (T0: d0, T1: d27, T2: d63, T3: d98) and carbon source (Cotton Glacier: CG, Pony Lake: PL, Suwannee River: SR). Creator(s): Tigges, Michelle; Bothner, Brian; Foreman, Christine Date Created: 2018-03-23 Repository: USAP-DC (current) License: Creative Commons Attribution-NonCommercial v4.0 Generic [CC BY-NC 4.0] Spatial Extent(s) West: 161.1667, East: 161.667, South: -77.117, North: -77.117 Temporal Extent(s) Start: 2013-10-15 - End: 2014-01-22 Award(s) NSF 1141978 Version: 1 Related Project(s) Multidimensional "omics" characterization of microbial metabolism and dissolved organic matter in Antarctica References Smith, H. J., Tigges, M. , D'Andrilli, J. , Parker, A. , Bothner, B. and Foreman, C. M. (2018), Dynamic processing of DOM: Insight from exometabolomics, fluorescence spectroscopy, and mass spectrometry. Limnol. Oceanogr., 3: 225-235 (doi:10.1002/lol2.10082) Keywords Antarctica Biota Biota Exometabolites Mass Spectrometry Microbes Microbiology