IEDA
Dataset Information
What Limits Denitrification and Bacterial Growth in Lake Bonney, Taylor Valley, Antarctica?
Data DOI:
https://doi.org/10.15784/600033
Cite as
Ward, B. (2009) "What Limits Denitrification and Bacterial Growth in Lake Bonney, Taylor Valley, Antarctica?" U.S. Antarctic Program (USAP) Data Center. doi: https://doi.org/10.15784/600033.
AMD - DIF Record(s)
Abstract
Denitrification is the main process by which fixed nitrogen is lost from ecosystems and the regulation of this process may directly affect primary production and carbon cycling over short and long time scales. Previous investigations of the role of bioactive metals in regulating denitrification in bacteria from permanently ice-covered Lake Bonney in the Taylor Valley of East Antarctica indicated that denitrifying bacteria can be negatively affected by metals such as copper, iron, cadmium, lead, chromium, nickel, silver and zinc; and that there is a distinct difference in denitrifying activity between the east and west lobes of the lake. Low iron concentrations were found to exacerbate the potential toxicity of the other metals, while silver has the potential to specifically inhibit denitrification because of its ability to interfere with copper binding in redox proteins, such as nitrite reductase and nitrous oxide reductase. High silver concentrations might prevent the functioning of nitrous oxide reductase in the same way that simple copper limitation does, thereby causing the buildup of nitrous oxide and resulting in a nonfunctional nitrogen cycle. Other factors, such as oxygen concentration, are likely also to affect bacterial activity in Lake Bonney. This project will investigate silver toxicity, general metal toxicity and oxygen concentration to determine their effect on denitrification in the lake by using a suite of 'sentinel' strains of denitrifying bacteria (isolated from the lake) incubated in Lake Bonney water and subjected to various treatments. The physiological responses of these strains to changes in metal and oxygen concentration will be quantified by flow cytometric detection of single cell molecular probes whose sensitivity and interpretation have been optimized for the sentinel strains. Understanding the relationships between metals and denitrification is expected to enhance our understanding of not only Lake Bonney's unusual nitrogen cycle, but more generally, of the potential role of metals in the regulation of microbial nitrogen transformations. The broader impacts of this work include not only a better understanding of regional biogeochemistry and global perspectives on these processes; but also the training of graduate students and a substantial outreach effort for school children.
Creator(s):
Date Created:
2009-01-01
Repository:
USAP-DC (current) - LDEO-LEGACY (original)
Spatial Extent(s)
West: 162, East: 163.6, South: -77.8, North: -77.2
Temporal Extent(s)
Start: 2003-09-15 - End: 2008-08-31
Award(s)
Version:
1
Data Files
Download
724 Bytes
2009-01-20/data.html

MD5 Checksum: 34b915320ca62bd38e99381679027f02 File Type: HyperText Markup Language (HTML)

Download
38.7 kB
2009-01-20/ELBCTD2004.xlsx

MD5 Checksum: 17e080d07f39db392b0d7754182449bc File Type: Microsoft Excel (OpenXML)

Download
78.3 kB
2009-01-20/ELBCTD2005.xlsx

MD5 Checksum: 7a7c834c8e70090f0682e3ab18071f0c File Type: Microsoft Excel (OpenXML)

Download
51.5 kB
2009-01-20/ELBOptode2004.xlsx

MD5 Checksum: cbd75be419c263ed0419c9d89c69d7b3 File Type: Microsoft Excel (OpenXML)

Download
52.1 kB
2009-01-20/ELBOptode2005.xlsx

MD5 Checksum: 846056851f64cd70ed3efec2a727bb03 File Type: Microsoft Excel (OpenXML)

Download
63.8 kB
2009-01-20/ELBTHY2005.xlsx

MD5 Checksum: b6bdc6db48d216c8b1031c916fd5ce26 File Type: Microsoft Excel (OpenXML)

Download
146 Bytes
2009-01-20/index.php

MD5 Checksum: 0cfe4eb7bc826dec5f9248cef4f2a710 File Type: Hypertext Preprocessor (Personal Home Page)

Download
754 Bytes
2009-01-20/README.txt

MD5 Checksum: e65fd39fe4a9959179b8d7e9eaa22c6d File Type: Readme Text File

Download
73.4 kB
2009-01-20/VandaCTD2004.xlsx

MD5 Checksum: 534990d0a93e25a229ecb186b1be56f3 File Type: Microsoft Excel (OpenXML)

Download
36.4 kB
2009-01-20/WLBCTD2004.xlsx

MD5 Checksum: b5f67544491114bf8f987f09bb902b6b File Type: Microsoft Excel (OpenXML)

Download
86.7 kB
2009-01-20/WLBCTD2005.xlsx

MD5 Checksum: b27cadca040725d3c61b1150659add55 File Type: Microsoft Excel (OpenXML)

Download
55.1 kB
2009-01-20/WLBOptode2004.xlsx

MD5 Checksum: 8c80b1966a69ea7d9da1e219de818fed File Type: Microsoft Excel (OpenXML)

Download
53.8 kB
2009-01-20/WLBOptode2005.xlsx

MD5 Checksum: b61bb0b4307f1451c6b60884d43e5247 File Type: Microsoft Excel (OpenXML)

Download
53.8 kB
2009-01-20/WLBTHY2005.xlsx

MD5 Checksum: 5a024ba2606d60ec51a01f24b1691e1d File Type: Microsoft Excel (OpenXML)

This dataset has been downloaded 0 times since March 2017 (based on unique date-IP combinations)