USAP-DC

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Metabarcoding (16S, 18S) ASV Table from Canada Glacier cryoconite sediments incubated with nutrients Data DOI: https://doi.org/10.15784/601957 Cite as Porazinska, D. (2026) "Metabarcoding (16S, 18S) ASV Table from Canada Glacier cryoconite sediments incubated with nutrients" U.S. Antarctic Program (USAP) Data Center. doi: https://doi.org/10.15784/601957. Abstract This dataset includes an Amplicon Sequence Variant (ASV) table derived from 16S and 18S metabarcoding of microbial communities exposed to nutrient enrichment under microcosm conditions. The original communities came from cryoconite hole sediments from Canada Glacier, Taylor Valley, Antarctica. Homogenized sediments were then distributed among 20 sterile Petri dishes (60 mm), each with 25 g dry weight equivalent (31.25 g wet sediment based on 25% water content) and assigned to five experimental treatments. Treatments consisted of 1) Bulk, sediments that received neither water nor nutrients, 2) Control, sediments that received additions of 1 ml of water, 3) +N, sediments that received 1 ml of ammonium nitrate solution to achieve 4 µmol N * g soil-1, 3) +P, sediments that received 1 ml of sodium phosphate dibasic solution to achieve 2 µmol P * g soil-1, and 5) +NP, sediments that received 1 ml of combined ammonium nitrate and sodium phosphate dibasic to achieve 4 µmol N + 2µmol P * g soil-1. Each treatment was replicated five times and incubated in a freeze-thaw growth chamber with temperatures cycling between 1°C and 3°C and light between ~100 Lux (5 hrs/day) and ~10,000 Lux (19 hrs per day) to imitate natural cryoconite holes conditions during austral summers. Creator(s): Porazinska, Dorota Date Created: 2025-07-23 Repository: USAP-DC (current) License: Creative Commons Attribution Only v4.0 Generic [CC BY 4.0] Temporal Extent(s) Start: 2022-04-27 - End: 2025-04-30 Award(s) NSF 2137376 Version: 1 Related Project(s) Collaborative Research: Role of Nutrient Limitation and Viral Interactions on Antarctic Microbial Community Assembly: A Cryoconite Microcosm Study Keywords Antarctica Cryosphere McMurdo Dry Valleys Microbial Diversity Taylor Valley