USAP-DC

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{"@context": "https://schema.org/", "@type": "Dataset", "@id": "doi:10.15784/601626", "additionalType": ["geolink:Dataset", "vivo:Dataset"], "name": "Flow cytometry enumeration of virus-like and bacteria-like abundance in Ace, Deep, & Organic lakes (Antarctica)", "description": "Varying depth samples from Ace, Deep, & Organic Lake were obtained between 2013-2014. These samples were size fractionated by filtration for metagenomic analyses. Viral and bacterial abundances were determine on the filtrate fractions by flow cytometric analysis. Glutaraldehyde-fixed samples were stained with SYBR Green I fluorescence dye and virus-like particle (VLP) and bacteria-like cell (BLC) populations were discriminated based on green fluorescence and side scatter flow cytometry signals. Several distinct VLP and BLC populations where enumerated from Ace Lake and Organic Lake samples that revealed temporal and depth differences. Discrete VLP and BLC populations could not be discerned from Deep Lake samples. These files include the original flow cytometry particle counts (raw data), the gating templates used to count VLP and BLC populations (analysis_templates), and the resulting particle counts (analyzed).", "citation": "Twining, B., & Martinez-Martinez, J. (2022) \"Flow cytometry enumeration of virus-like and bacteria-like abundance in Ace, Deep, & Organic lakes (Antarctica)\" U.S. Antarctic Program (USAP) Data Center. doi: https://doi.org/10.15784/601626.", "datePublished": "2022-12-08", "keywords": ["Ace Lake", "Antarctica", "Antarctica", "Deep Lake", "Organic Lake", "Vestfold Hills"], "creator": [{"@type": "Person", "additionalType": "geolink:Person", "name": "Martinez-Martinez, Joaquin", "email": null, "affiliation": {"@type": "Organization", "name": null}}, {"@type": "Person", "additionalType": "geolink:Person", "name": "Twining, Benjamin", "email": "btwining@bigelow.org", "affiliation": {"@type": "Organization", "name": "Bigelow Laboratory for Ocean Sciences"}}], "distribution": [{"@type": "DataDownload", "additionalType": "http://www.w3.org/ns/dcat#DataCatalog", "encodingFormat": "text/xml", "name": "ISO Metadata Document", "url": "https://www.usap-dc.org/metadata/isoxml/601626iso.xml", "contentUrl": "/dataset/filename"}, {"@type": "DataDownload", "@id": "http://dx.doi.org/10.15784/601626", "additionalType": "dcat:distribution", "url": "http://dx.doi.org/10.15784/601626", "contentUrl": "/dataset/filename", "encodingFormat": "text/html"}, {"@type": "DataDownload", "@id": "https://www.usap-dc.org/view/dataset/601626", "additionalType": "dcat:distribution", "url": "https://www.usap-dc.org/view/dataset/601626", "contentUrl": "/dataset/filename", "encodingFormat": "text/html"}], "identifier": {"@type": "PropertyValue", "propertyID": "https://registry.identifiers.org/registry/doi", "value": "doi:10.15784/601626", "url": "https://doi.org/10.15784/601626"}, "contributor": [{"@type": "Role", "roleName": "credit", "description": "funderName:NSF:GEO:OPP:Antarctic Organisms and Ecosystems awardNumber:1644155 awardTitle:Viral control of microbial communities in Antarctic lakes"}], "license": [{"@type": "CreativeWork", "URL": "https://creativecommons.org/licenses/by/4.0/", "name": "MD_Constraints", "description": "useLimitation: Creative Commons Attribution Only v4.0 Generic [CC BY 4.0]."}, {"@type": "CreativeWork", "name": "MD_LegalConstraints", "description": "accessConstraints: license. otherConstraints: Creative Commons Attribution Only v4.0 Generic [CC BY 4.0]."}, {"@type": "CreativeWork", "name": "MD_SecurityConstraints", "description": "classification: "}], "publisher": {"@type": "Organization", "name": "U.S. Antarctic Program (USAP) Data Center"}, "spatialCoverage": [{"@type": "Place", "geo": {"@type": "GeoShape", "box": "-68.4667, -78.1833, -68.4333, -78.0002"}}]}

Flow cytometry enumeration of virus-like and bacteria-like abundance in Ace, Deep, & Organic lakes (Antarctica) Data DOI: https://doi.org/10.15784/601626 Cite as Twining, B., & Martinez-Martinez, J. (2022) "Flow cytometry enumeration of virus-like and bacteria-like abundance in Ace, Deep, & Organic lakes (Antarctica)" U.S. Antarctic Program (USAP) Data Center. doi: https://doi.org/10.15784/601626. AMD - DIF Record(s) USAP-1644155_1 Abstract Varying depth samples from Ace, Deep, & Organic Lake were obtained between 2013-2014. These samples were size fractionated by filtration for metagenomic analyses. Viral and bacterial abundances were determine on the filtrate fractions by flow cytometric analysis. Glutaraldehyde-fixed samples were stained with SYBR Green I fluorescence dye and virus-like particle (VLP) and bacteria-like cell (BLC) populations were discriminated based on green fluorescence and side scatter flow cytometry signals. Several distinct VLP and BLC populations where enumerated from Ace Lake and Organic Lake samples that revealed temporal and depth differences. Discrete VLP and BLC populations could not be discerned from Deep Lake samples. These files include the original flow cytometry particle counts (raw data), the gating templates used to count VLP and BLC populations (analysis_templates), and the resulting particle counts (analyzed). Creator(s): Twining, Benjamin; Martinez-Martinez, Joaquin Date Created: 2022-12-12 Repository: USAP-DC (current) License: Creative Commons Attribution Only v4.0 Generic [CC BY 4.0] Spatial Extent(s) West: -78.1833, East: -78.0002, South: -68.4667, North: -68.4333 Award(s) NSF 1644155 Version: 1 Related Project(s) Viral control of microbial communities in Antarctic lakes Keywords Ace Lake Antarctica Antarctica Deep Lake Organic Lake Vestfold Hills