USAP-DC

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Multidimensional "omics" characterization of microbial metabolism and dissolved organic matter in Antarctica Start Date: 2012-09-01 End Date: 2015-08-31 Description/Abstract Uncovering the dynamics of dissolved organic matter (DOM) is central to an understanding of the global carbon cycle, as organic material from lakes, streams, oceans and soils passes through this pool. DOM acts as a key energy source for microbes in many ecosystems and therefore can affect regional nutrient cycling patterns. For example, preliminary results suggest that organisms isolated from a supraglacial stream on Cotton Glacier, Antarctica, may be important in DOM cycling in this relatively simple, low temperature system. However, little is known about the functional attributes of the microbes that interact with DOM in the environment. This project will use state-of-the-art genomics, proteomics and metabolomics approaches to understand the mechanisms by which two microbial isolates, CG3 and CG9_1, affect DOM cycling. Liquid chromatography-mass spectrometry will also be used to better characterize the microbially-derived DOM from this ecosystem. This project will support the research and training of one undergraduate and two graduate students. Results will be widely disseminated through publications as well as through presentations at national and international meetings. In addition, raw data will be made available through open-access databases. Understanding the relationship between cold-adapted microbial metabolisms and DOM pools is important as more than 90% of the Earth?s oceans are below 5 degrees Celsius. Personnel Person Role Foreman, Christine Investigator Bothner, Brian Co-Investigator Funding Antarctic Organisms and Ecosystems Award # 1141978 AMD - DIF Record(s) USAP-1141978 Data Management Plan None in the Database Product Level: Not provided Datasets Repository Title (link) Format(s) Status USAP-DC Respiration Metadata None exist USAP-DC FT-ICR MS Metadata None exist USAP-DC UPLC-Q-TOF data of Cotton Glacier exometabolites None exist Publications Smith, H. J., Tigges, M. , D'Andrilli, J. , Parker, A. , Bothner, B. and Foreman, C. M. (2018), Dynamic processing of DOM: Insight from exometabolomics, fluorescence spectroscopy, and mass spectrometry. Limnol. Oceanogr., 3: 225-235 (doi:10.1002/lol2.10082) Smith, H., Dieser, M., McKnight, D., SanClements, M., & Foreman, C. (2018). Relationship between dissolved organic matter quality and microbial community composition across polar glacial environments. FEMS Microbiology Ecology, 94(7). (doi:10.1093/femsec/fiy090) Smith, H. J., Foreman, C. M., Akiyama, T., Franklin, M. J., Devitt, N. P., & Ramaraj, T. (2016). Genome Sequence of Janthinobacterium sp. CG23_2, a Violacein-Producing Isolate from an Antarctic Supraglacial Stream. Genome Announcements, 4(1). (doi:10.1128/genomea.01468-15) Platforms and Instruments