USAP-DC

{"dp_type": "Project", "free_text": "molecular"}

[{"awards": "2240780 Cohen, Natalie", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": null, "datasets": null, "date_created": "Mon, 13 Mar 2023 00:00:00 GMT", "description": "Mixotrophic microorganisms that are capable of both photosynthetic and heterotrophic forms of metabolism are key contributors to primary productivity and organic carbon remineralization in the Southern Ocean. However, uncertainties in their grazing behavior and physiology prevent an accurate understanding of microbial food web dynamics and biogeochemical cycling in the Antarctic ecosystem. Polar mixotrophs have evolved to withstand extreme seasonality, including variable light, sea ice, temperature, and micronutrient concentrations. In particular, the Southern Ocean appears to be the only region of the world\u2019s ocean where the bioessential trace metals iron (Fe) and manganese (Mn) are low enough to inhibit photosynthetic growth. The molecular physiology of mixotrophs experiencing Fe and Mn growth limitation has not yet been examined, and we lack an understanding of how seasonal changes in the availability of these micronutrients influence mixotrophic growth dynamics. We aim to examine whether grazing affords mixotrophs an ecological advantage in the Fe and Mn-deficient Southern Ocean, and to characterize the shifts in metabolic processes that occur during transitions in micronutrient conditions. Culture studies will directly measure growth responses, grazing behavior, and changes in elemental stoichiometry in response to Fe and Mn limitation. Transcriptomic analyses will reveal the metabolic underpinnings of trophic behavior and micronutrient stress responses, with implications for key biogeochemical processes such as carbon fixation, remineralization, and nutrient cycling. ", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "PLANKTON; Georgia; PHYTOPLANKTON", "locations": "Georgia", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Cohen, Natalie; Millette, Nicole", "platforms": null, "repositories": null, "science_programs": null, "south": -90.0, "title": "ANT LIA: Collaborative Research: Mixotrophic Grazing as a Strategy to meet Nutritional Requirements in the Iron and Manganese Deficient Southern Ocean", "uid": "p0010411", "west": -180.0}, {"awards": "2132641 Bik, Holly", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -62,180 -64,180 -66,180 -68,180 -70,180 -72,180 -74,180 -76,180 -78,180 -80,144 -80,108 -80,72 -80,36 -80,0 -80,-36 -80,-72 -80,-108 -80,-144 -80,-180 -80,-180 -78,-180 -76,-180 -74,-180 -72,-180 -70,-180 -68,-180 -66,-180 -64,-180 -62,-180 -60))", "dataset_titles": null, "datasets": null, "date_created": "Tue, 30 Aug 2022 00:00:00 GMT", "description": "Nematode worms are abundant and ubiquitous in marine sediment habitats worldwide, performing key functions such as nutrient cycling and sediment stability. However, study of this phylum suffers from a perpetual and severe taxonomic deficit, with less than 5,000 formally described marine species. Fauna from the Southern Ocean are especially poorly studied due to limited sampling and the general inaccessibility of the Antarctic benthos. This study is providing the first large-scale molecular-based investigation from marine nematodes in the Eastern Antarctic continental shelf, providing an important comparative dataset for the existing body of historical (morphological) taxonomic studies. This project uses a combination of classical taxonomy (microscopy) and modern -omics tools to achieve three overarching aims: 1) determine if molecular data supports high biodiversity and endemism of benthic meiofauna in Antarctic benthic ecosystems; 2) determine the proportion of marine nematode species that have a deep-sea versus shallow-water evolutionary origin on the Antarctic shelf, and assess patterns of cryptic speciation in the Southern Ocean; and 3) determine the most important drivers of the host-associated microbiome in Antarctic marine nematodes. This project is designed to rapidly advance knowledge of the evolutionary origins of Antarctic meiofauna, provide insight on population-level patterns within key indicator genera, and elucidate the potential ecological and environmental factors which may influence microbiome patterns. Broader Impacts activities include an intensive cruise- and land-based outreach program focusing on social media engagement and digital outreach products, raising awareness of Antarctic marine ecosystems and understudied microbial-animal relationships. The diverse research team includes female scientists, first-generation college students, and Latinx trainees.\r\n", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "East Antarctica; BENTHIC", "locations": "East Antarctica", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Bik, Holly", "platforms": null, "repositories": null, "science_programs": null, "south": -80.0, "title": "ANT LIA: Do Molecular Data Support High Endemism and Divergent Evolution of Antarctic Marine Nematodes and their Host-associated Microbiomes?", "uid": "p0010372", "west": -180.0}, {"awards": "2002422 TBD", "bounds_geometry": null, "dataset_titles": "Clumped-isotope composition of molecular oxygen trapped in the Allan Hills S27 ice core", "datasets": [{"dataset_uid": "601593", "doi": "10.15784/601593", "repository": "USAP-DC", "science_program": "Allan Hills", "title": "Clumped-isotope composition of molecular oxygen trapped in the Allan Hills S27 ice core", "url": "http://www.usap-dc.org/view/dataset/601593"}], "date_created": "Tue, 02 Aug 2022 00:00:00 GMT", "description": null, "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "Allan Hills; Antarctica; Last Interglacial; Oxygen", "locations": "Allan Hills; Antarctica", "north": null, "nsf_funding_programs": null, "paleo_time": null, "persons": "Banerjee, Asmita; Yan, Yuzhen; Yeung, Laurence", "platforms": null, "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": "Allan Hills", "south": null, "title": null, "uid": null, "west": null}, {"awards": "1937546 Morgan-Kiss, Rachael; 1937595 Briggs, Brandon", "bounds_geometry": "POLYGON((162 -77.616667,162.1 -77.616667,162.2 -77.616667,162.3 -77.616667,162.4 -77.616667,162.5 -77.616667,162.6 -77.616667,162.7 -77.616667,162.8 -77.616667,162.9 -77.616667,163 -77.616667,163 -77.6283336,163 -77.6400002,163 -77.6516668,163 -77.6633334,163 -77.67500000000001,163 -77.68666660000001,163 -77.69833320000001,163 -77.7099998,163 -77.7216664,163 -77.733333,162.9 -77.733333,162.8 -77.733333,162.7 -77.733333,162.6 -77.733333,162.5 -77.733333,162.4 -77.733333,162.3 -77.733333,162.2 -77.733333,162.1 -77.733333,162 -77.733333,162 -77.7216664,162 -77.7099998,162 -77.69833320000001,162 -77.68666660000001,162 -77.67500000000001,162 -77.6633334,162 -77.6516668,162 -77.6400002,162 -77.6283336,162 -77.616667))", "dataset_titles": null, "datasets": null, "date_created": "Wed, 27 Jul 2022 00:00:00 GMT", "description": " Microbial communities are of more than just a scientific curiosity. Microbes represent the single largest source of evolutionary and biochemical diversity on the planet. They are the major agents for cycling carbon, nitrogen, phosphorus, and other elements through the ecosystem. Despite their importance in ecosystem function, microbes are still generally overlooked in food web models and nutrient cycles. Moreover, microbes do not live in isolation: their growth and metabolism are influenced by complex interactions with other microorganisms. This project will focus on the ecology, activity and roles of microbial communities in Antarctic Lake ecosystems. The team will characterize the genetic underpinnings of microbial interactions and the influence of environmental gradients (e.g. light, nutrients, oxygen, sulfur) and seasons (e.g. summer vs. winter) on microbial networks in Lake Fryxell and Lake Bonney in the Taylor Valley within the McMurdo Dry Valley region. Finally, the project furthers the NSF goals of training new generations of scientists by including undergraduate and graduate students, a postdoctoral researcher and a middle school teacher in both lab and field research activities. This partnership will involve a number of other outreach training activities, including visits to classrooms and community events, participation in social media platforms, and webinars. \u003cbr/\u003e\u003cbr/\u003ePart II: Technical description: Ecosystem function in the extreme Antarctic Dry Valleys ecosystem is dependent on complex biogeochemical interactions between physiochemical environmental factors (e.g. light, nutrients, oxygen, sulfur), time of year (e.g. summer vs. winter) and microbes. Microbial network complexity can vary in relation to specific abiotic factors, which has important implications on the fragility and resilience of ecosystems under threat of environmental change. This project will evaluate the influence of biogeochemical factors on microbial interactions and network complexity in two Antarctic ice-covered lakes. The study will be structured by three main objectives: 1) infer positive and negative interactions from rich spatial and temporal datasets and investigate the influence of biogeochemical gradients on microbial network complexity using a variety of molecular approaches; 2) directly observe interactions among microbial eukaryotes and their partners using flow cytometry, single-cell sorting and microscopy; and 3) develop metabolic models of specific interactions using metagenomics. Outcomes from amplicon sequencing, meta-omics, and single-cell genomic approaches will be integrated to map specific microbial network complexity and define the role of interactions and metabolic activity onto trends in limnological biogeochemistry in different seasons. These studies will be essential to determine the relationship between network complexity and future climate conditions. Undergraduate researchers will be recruited from both an REU program with a track record of attracting underrepresented minorities and two minority-serving institutions. To further increase polar literacy training and educational impacts, the field team will include a teacher as part of a collaboration with the successful NSF-funded PolarTREC program and participation in activities designed for public outreach.\u003cbr/\u003e\u003cbr/\u003eThis award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 163.0, "geometry": "POINT(162.5 -77.67500000000001)", "instruments": null, "is_usap_dc": true, "keywords": "MICROALGAE; AQUATIC ECOSYSTEMS; Antarctica; LAKE/POND; BACTERIA/ARCHAEA; COMMUNITY DYNAMICS", "locations": "Antarctica", "north": -77.616667, "nsf_funding_programs": "Antarctic Integrated System Science; Antarctic Integrated System Science", "paleo_time": null, "persons": "Morgan-Kiss, Rachael; Briggs, Brandon", "platforms": null, "repositories": null, "science_programs": null, "south": -77.733333, "title": "ANT LIA: Collaborative Research: Genetic Underpinnings of Microbial Interactions in Chemically Stratified Antarctic Lakes", "uid": "p0010355", "west": 162.0}, {"awards": "2055455 Duhaime, Melissa", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": null, "datasets": null, "date_created": "Fri, 03 Jun 2022 00:00:00 GMT", "description": "Part 1: Non-technical description:\r\nIt is well known that the Southern Ocean plays an important role in global carbon cycling and also receives a disproportionately large influence of climate change. The role of marine viruses on ocean productivity is largely understudied, especially in this global region. This team proposes to use combination of genomics, flow cytometry, and network modeling to test the hypothesis that viral biogeography, infection networks, and viral impacts on microbial metabolism can explain variations in net community production (NCP) and carbon cycling in the Southern Ocean. The project includes the training of a postdoctoral scholar, graduate students and undergraduate students. It also includes the development of a new Polar Sci ReachOut program in partnership with the University of Michigan Museum of Natural History especially targeted to middle-school students and teachers and the general public. The team will also produce a Science for Tomorrow (SFT) program for use in middle schools in metro-Detroit communities and lead a summer Research Experience for Teachers (RET) fellows. \r\n\r\nPart 2: Technical description: \r\nThe study will leverage hundreds of existing samples collected for microbes and viruses from the Antarctic Circumpolar Expedition (ACE). These samples provide the first contiguous survey of viral diversity and microbial communities around Antarctica. Viral networks are being studied in the context of biogeochemical data to model community networks and predict net community production (NCP), which will provide a way to evaluate the role of viruses in Southern Ocean carbon cycling. Using cutting edge molecular and flow cytometry approaches, this project addresses the following questions: 1) How/why are Southern Ocean viral populations distributed across environmental gradients? 2a) Do viruses interfere with \"keystone\" metabolic pathways and biogeochemical processes of microbial communities in the Southern Ocean? 2b) Does nutrient availability or other environmental variables drive changes in virus-microbe infection networks in the Southern Ocean? Results will be used to develop and evaluate generative models of NCP predictions that incorporate the importance of viral traits and virus-host interactions.\r\n\r\nThis award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "Southern Ocean; Amd/Us; AMD; FIELD INVESTIGATION; USA/NSF; AQUATIC SCIENCES; BACTERIA/ARCHAEA; MARINE ECOSYSTEMS; VIRUSES; USAP-DC", "locations": "Southern Ocean", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Duhaime, Melissa; Zaman, Luis", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION", "repositories": null, "science_programs": null, "south": -90.0, "title": "ANT LIA - Viral Ecogenomics of the Southern Ocean: Unifying Omics and Ecological Networks to Advance our Understanding of Antarctic Microbial Ecosystem Function", "uid": "p0010333", "west": -180.0}, {"awards": "1443637 Zakon, Harold", "bounds_geometry": null, "dataset_titles": "Evolutionary analysis of transient receptor potential (TRP) channels in notothenioid fishes; Functional characterization of temperature activated ion channels from Antarctic fishes; TagSeq tissue specific expression data for Antarctic Harpagifer antarcticus and tropical African cichlid Astatotilapia (Haplochromis) burtoni", "datasets": [{"dataset_uid": "200292", "doi": "10.18738/T8/NXGNEI", "keywords": null, "people": null, "repository": "Texas Data Repository", "science_program": null, "title": "Evolutionary analysis of transient receptor potential (TRP) channels in notothenioid fishes", "url": "https://doi.org/10.18738/T8/NXGNEI"}, {"dataset_uid": "601695", "doi": "10.15784/601695", "keywords": "Antarctica; Notothenioid; Southern Ocean", "people": "York, Julia", "repository": "USAP-DC", "science_program": null, "title": "Functional characterization of temperature activated ion channels from Antarctic fishes", "url": "https://www.usap-dc.org/view/dataset/601695"}, {"dataset_uid": "200293", "doi": "", "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "TagSeq tissue specific expression data for Antarctic Harpagifer antarcticus and tropical African cichlid Astatotilapia (Haplochromis) burtoni", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA758918"}], "date_created": "Fri, 03 Jun 2022 00:00:00 GMT", "description": "We studied the molecular evolution and physiology of two types of ion channels: voltage gated potassium channels and transient receptor potential (TRP) channels. We also studied the molecular evolution and expression of water-passing channels, the aquaporins, to determine if these show signs of evolutionary change in notothenioids. \r\n\r\nWe noted apparent amino acid substitutions at a number of sites in a muscle-expressing\r\npotassium channel (Kv1.3). We were surprised to find that although the AAs at these sites\r\nappeared highly conserved in teleosts and even in tetrapods, reverting them singly, in pairs,\r\nor all together back to the ancestral condition had no effect on the biophysical properties of\r\nthe channels that we measured (voltage-sensitivity; rate of activation) at room temperature\r\nas well as over a range of temperatures down to 4oC.\r\n\r\nThe results for the TRP channels and aquaporins can be accessed in their publications. York and Zakon (2022) in Genome Biology and Evolution, and two forthcoming papers.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "Amd/Us; USAP-DC; USA/NSF; FIELD INVESTIGATION; AMD; FISHERIES", "locations": null, "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Zakon, Harold", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION", "repo": "Texas Data Repository", "repositories": "GenBank; Texas Data Repository; USAP-DC", "science_programs": null, "south": null, "title": "Analysis of Voltage-gated Ion Channels in Antarctic Fish", "uid": "p0010331", "west": null}, {"awards": "2053726 Hofmann, Gretchen", "bounds_geometry": "POLYGON((163 -77,163.4 -77,163.8 -77,164.2 -77,164.6 -77,165 -77,165.4 -77,165.8 -77,166.2 -77,166.6 -77,167 -77,167 -77.1,167 -77.2,167 -77.3,167 -77.4,167 -77.5,167 -77.6,167 -77.7,167 -77.8,167 -77.9,167 -78,166.6 -78,166.2 -78,165.8 -78,165.4 -78,165 -78,164.6 -78,164.2 -78,163.8 -78,163.4 -78,163 -78,163 -77.9,163 -77.8,163 -77.7,163 -77.6,163 -77.5,163 -77.4,163 -77.3,163 -77.2,163 -77.1,163 -77))", "dataset_titles": "Analyses combining ATAC-seq, RRBS, and RNA-seq data for purple urchins", "datasets": [{"dataset_uid": "200288", "doi": "", "keywords": null, "people": null, "repository": "GitHub", "science_program": null, "title": "Analyses combining ATAC-seq, RRBS, and RNA-seq data for purple urchins", "url": "https://github.com/snbogan/Sp_RRBS_ATAC"}], "date_created": "Thu, 14 Apr 2022 00:00:00 GMT", "description": "Part 1: Non-technical description:\r\n\tWith support from the Office of Polar Programs, this project will evaluate how an important part of the food web in the coastal ocean of Antarctica will respond to climate change. The focal study organism in the plankton is a shelled mollusk, the Antarctic pteropod, Limacina helicina antarctica, a Southern Ocean organism that this known to respond to climate driven changes in ocean acidification and ocean warming. Ocean acidification, the lowering of ocean pH via the absorption of atmospheric carbon dioxide in the surface of the ocean, is a change in the ocean that is expected to cross deleterious thresholds of pH within decades. This study will improve understanding of how pteropods will respond, which will provide insight into predicting the resilience of the Antarctic marine ecosystem during future changes, one of the planets last marine wildernesses. The project will use tools of molecular biology to examine specifically how gene expression is modulated in the pteropods, and further, how the changes and regulation of genes act to resist the stress of low pH and high temperature. In addition, this project supports the training of Ph.D. graduate students and advances the goal of inclusive excellence in STEM and in marine sciences, in particular. The students involved in this project are from groups traditionally under-represented in marine science including first-generation college students. Overall, the project contributes to the development of the U.S. work force and contributes to diversity and inclusive excellence in the geosciences.\r\n\r\nPart 2: Technical description: \r\nThe overarching goal of this project is to investigate the molecular response of the Antarctic thecosome pteropod, Limacina helicina antarctica to ocean acidification (OA) and ocean warming. The project will investigate changes in the epigenome of juvenile L. h. antarctica, by assessing the dynamics of DNA methylation in response to three scenarios of environmental conditions that were simulated in laboratory mesocosm CO2 experiments: (1) present-day pCO2 conditions for summer and winter, (2) future ocean acidification expected within 10-15 years, and (3) a multiple stressor experiment to investigate synergistic interaction of OA and high temperature stress. Recent lab-based mesocosm experiment research showed significant changes in the dynamics of global DNA methylation in the pteropod genome, along with variation in gene expression in response to abiotic changes. Thus, it is clear that juvenile L. h. antarctica are capable of mounting a substantial epigenetic response to ocean acidification. However, it is not known how DNA methylation, as an epigenetic process, is modulating changes in the transcriptome. In order to address this gap in the epigenetic knowledge regarding pteropods, the project will use next-generation sequencing approaches (e.g., RNA sequencing and reduced representation bisulfite sequencing) to integrate changes in methylation status with changes in gene expression in juvenile pteropods. Overall, this investigation is an important step in exploring environmental transcriptomics and phenotypic plasticity of an ecologically important member of Southern Ocean macrozoooplankton in response to anthropogenic climate change.\r\n\r\nThis award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 167.0, "geometry": "POINT(165 -77.5)", "instruments": null, "is_usap_dc": true, "keywords": "USAP-DC; Amd/Us; FIELD INVESTIGATION; USA/NSF; AMD; Mcmurdo Sound; MARINE ECOSYSTEMS; ANIMALS/INVERTEBRATES", "locations": "Mcmurdo Sound", "north": -77.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Hofmann, Gretchen", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION", "repo": "GitHub", "repositories": "GitHub", "science_programs": null, "south": -78.0, "title": "The Role of the Epigenetic Mechanism, DNA Methylation, in the Tolerance and Resistance of Antarctic Pteropods to Ocean Acidification and Warming", "uid": "p0010313", "west": 163.0}, {"awards": "2020664 Vazquez-Medina, Jose Pablo; 2020706 Hindle, Allyson", "bounds_geometry": "POLYGON((164 -77.2,164.3 -77.2,164.6 -77.2,164.9 -77.2,165.2 -77.2,165.5 -77.2,165.8 -77.2,166.1 -77.2,166.4 -77.2,166.7 -77.2,167 -77.2,167 -77.265,167 -77.33,167 -77.395,167 -77.46,167 -77.525,167 -77.59,167 -77.655,167 -77.72,167 -77.785,167 -77.85,166.7 -77.85,166.4 -77.85,166.1 -77.85,165.8 -77.85,165.5 -77.85,165.2 -77.85,164.9 -77.85,164.6 -77.85,164.3 -77.85,164 -77.85,164 -77.785,164 -77.72,164 -77.655,164 -77.59,164 -77.525,164 -77.46,164 -77.395,164 -77.33,164 -77.265,164 -77.2))", "dataset_titles": null, "datasets": null, "date_created": "Thu, 09 Sep 2021 00:00:00 GMT", "description": "The Weddell seal is a champion diver with high natural tolerance for low blood oxygen concentration (hypoxemia) and inadequate blood supply (ischemia). The processes unique to this species protects their tissues from inflammation and oxidative stress observed in other mammalian tissues exposed to such physiological conditions. This project aims to understand the signatures of the processes that protect seals from inflammation and oxidant stress, using molecular, cellular and metabolic tools. Repetitive short dives before long ones are hypothesized to precondition seal tissues and activate the protective processes. The new aspect of this work is the study of endothelial cells, which sense changes in oxygen and blood flow, providing a link between breath-holding and cellular function. The approach is one of laboratory experiments combined with 2-years of field work in an ice camp off McMurdo Station in Antarctica. The study is structured by three main objectives: 1) laboratory experiments with arterial endothelial cells exposed to changes in oxygen and flow to identify molecular pathways responsible for tolerance of hypoxia and ischemia using several physiological, biochemical and genomic tools including CRSPR/Cas9 knockout and knockdown approaches. 2) Metabolomic analyses of blood metabolites produced by seals during long dives. And 3) Metabolomic and genomic determinations of seal physiology during short dives hypothesized to pre-condition tolerance responses. In the field, blood samples will be taken after seals dive in an isolated ice hole and its diving performance recorded. It is expected that the blood will contain metabolites that can be related to molecular pathways identified in lab experiments. ", "east": 167.0, "geometry": "POINT(165.5 -77.525)", "instruments": null, "is_usap_dc": true, "keywords": "Mcmurdo Sound; FIELD SURVEYS; USA/NSF; AMD; MAMMALS; Amd/Us", "locations": "Mcmurdo Sound", "north": -77.2, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Hindle, Allyson", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS", "repositories": null, "science_programs": null, "south": -77.85, "title": "Collaborative Research: Role of Endothelial Cell Activation in Hypoxia Tolerance of an Elite Diver, the Weddell Seal", "uid": "p0010257", "west": 164.0}, {"awards": "1746087 Tarrant, Ann", "bounds_geometry": "POLYGON((-80 -60,-77.5 -60,-75 -60,-72.5 -60,-70 -60,-67.5 -60,-65 -60,-62.5 -60,-60 -60,-57.5 -60,-55 -60,-55 -61,-55 -62,-55 -63,-55 -64,-55 -65,-55 -66,-55 -67,-55 -68,-55 -69,-55 -70,-57.5 -70,-60 -70,-62.5 -70,-65 -70,-67.5 -70,-70 -70,-72.5 -70,-75 -70,-77.5 -70,-80 -70,-80 -69,-80 -68,-80 -67,-80 -66,-80 -65,-80 -64,-80 -63,-80 -62,-80 -61,-80 -60))", "dataset_titles": "Calanoides acutus: Transcriptome and gene expression data; BioProject PRJNA757455; Calanus propinquus: Transcriptome and gene expression data; BioProject PRJNA669816; Expedition data of LMG1901; Rhincalanus gigas: Transcriptome and gene expression data; BioProject PRJNA666170", "datasets": [{"dataset_uid": "200284", "doi": "", "keywords": null, "people": null, "repository": "NCBI", "science_program": null, "title": "Calanoides acutus: Transcriptome and gene expression data; BioProject PRJNA757455", "url": "https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA757455"}, {"dataset_uid": "200125", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG1901", "url": "https://www.rvdata.us/search/cruise/LMG1901"}, {"dataset_uid": "200239", "doi": "", "keywords": null, "people": null, "repository": "NCBI", "science_program": null, "title": "Rhincalanus gigas: Transcriptome and gene expression data; BioProject PRJNA666170", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA666170"}, {"dataset_uid": "200283", "doi": "", "keywords": null, "people": null, "repository": "NCBI", "science_program": null, "title": "Calanus propinquus: Transcriptome and gene expression data; BioProject PRJNA669816", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA669816"}], "date_created": "Fri, 06 Aug 2021 00:00:00 GMT", "description": "Polar marine organisms have adapted to dramatic seasonal changes in photoperiod, light intensity, and ice cover, as well as to cold but stable thermal environments. The western Antarctic Peninsula, the focal region for the field studies, has experienced rapid warming and ice melt. While it is difficult to predict exactly how physical conditions in this region will change, effects on species distributions have already been documented. Large Antarctic copepods in the families Calanidae and Rhincalanidae are dominant components of the mesozooplankton that use different metabolic and behavioral strategies to optimize their use of a highly seasonal food supply. The overall goal of this project is to leverage molecular approaches to examine the physiological and metabolic adaptations at the individual and species level. The project focuses on three main objectives: the first objective is to characterize the gene complement and stage-specific gene expression patterns in Antarctic copepods within an evolutionary context. The second objective is to measure and compare the physiological and molecular responses of juvenile copepods to variable feeding conditions. The third objective is to characterize metabolic variation within natural copepod populations. The metabolically diverse Antarctic copepods also provide an excellent opportunity to compare mechanisms regulating energy storage and utilization and to test hypotheses regarding the roles of specific genes. The field studies will aim to utilize information from an ongoing long term research program (the Palmer Long-Term Ecological Research), which complements the ongoing program and provides extensive context for this project. To make the data more useful to the research community, a database will be developed facilitating comparison of transcriptomes between copepod species. This project will provide hands-on training opportunities to graduate and undergraduate students. Efforts will be made to recruit students who are members of underrepresented minorities. Results and scientific concepts will be broadly disseminated through an expedition blog, undergraduate student programs, and public presentations.\r\n", "east": -55.0, "geometry": "POINT(-67.5 -65)", "instruments": null, "is_usap_dc": true, "keywords": "ARTHROPODS; AMD; PELAGIC; USA/NSF; USAP-DC; PLANKTON; West Antarctic Shelf; Amd/Us; SHIPS", "locations": "West Antarctic Shelf", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Tarrant, Ann", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e SHIPS", "repo": "NCBI", "repositories": "NCBI; R2R", "science_programs": null, "south": -70.0, "title": "Physiological Ecology of \"Herbivorous\" Antarctic Copepods", "uid": "p0010239", "west": -80.0}, {"awards": "1935635 Santagata, Scott; 1935672 Ryan, Joseph", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": null, "datasets": null, "date_created": "Mon, 28 Jun 2021 00:00:00 GMT", "description": "Understanding the genomic changes underlying adaptations to polar environments is critical for \r\npredicting how ecological changes will affect life in these fragile environments. Accomplishing these goals requires looking in detail at genome-scale data across a wide array of organisms in a phylogenetic framework. This study combines multifaceted computational and functional approaches that involves analyzing in the genic evolution of invertebrate organisms, known as the bryozoans or ectoprocts. In addition, the commonality of our results in other taxa will be tested by comparing the results to those produced from the previous and newly proposed workshops. Specific aims of this study include: 1) identifying genes involved in adaptation to Antarctic marine environments using transcriptomic and genomic data from bryozoans to test for positively selected genes in a phylogenetic framework, 2) experimentally testing identified candidate enzymes (especially those involved in calcium signaling, glycolysis, the citric acid cycle, and the cytoskeleton) for evidence of cold adaption, and 3) conducting computational workshops aimed at training scientists in techniques for the identification of genetic adaptations to polar and other disparate environments. The proposed work provides critical insights into the molecular rules of life in rapidly changing Antarctic environments, and provides important information for understanding how Antarctic taxa will respond to future environmental conditions.\r\n", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "USAP-DC; USA/NSF; Ross Sea; Ant Lia; ANIMALS/INVERTEBRATES; FIELD SURVEYS; Weddell Sea; Bellingshausen Sea; Amundsen Sea; Antarctic Peninsula; Amd/Us; AMD", "locations": "Amundsen Sea; Antarctic Peninsula; Bellingshausen Sea; Ross Sea; Weddell Sea", "north": -60.0, "nsf_funding_programs": "Antarctic Integrated System Science; Antarctic Organisms and Ecosystems; Antarctic Integrated System Science", "paleo_time": null, "persons": "Ryan, Joseph; Santagata, Scott", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS", "repositories": null, "science_programs": null, "south": -90.0, "title": "ANT LIA Collaborative Research: Interrogating Molecular and Physiological Adaptations in Antarctic Marine Animals.", "uid": "p0010212", "west": -180.0}, {"awards": "1850988 Teets, Nicholas", "bounds_geometry": "POLYGON((-64.366767 -62.68104,-63.9917036 -62.68104,-63.6166402 -62.68104,-63.2415768 -62.68104,-62.8665134 -62.68104,-62.49145 -62.68104,-62.1163866 -62.68104,-61.7413232 -62.68104,-61.3662598 -62.68104,-60.9911964 -62.68104,-60.616133 -62.68104,-60.616133 -62.9537037,-60.616133 -63.2263674,-60.616133 -63.4990311,-60.616133 -63.7716948,-60.616133 -64.0443585,-60.616133 -64.3170222,-60.616133 -64.5896859,-60.616133 -64.8623496,-60.616133 -65.1350133,-60.616133 -65.407677,-60.9911964 -65.407677,-61.3662598 -65.407677,-61.7413232 -65.407677,-62.1163866 -65.407677,-62.49145 -65.407677,-62.8665134 -65.407677,-63.2415768 -65.407677,-63.6166402 -65.407677,-63.9917036 -65.407677,-64.366767 -65.407677,-64.366767 -65.1350133,-64.366767 -64.8623496,-64.366767 -64.5896859,-64.366767 -64.3170222,-64.366767 -64.0443585,-64.366767 -63.7716948,-64.366767 -63.4990311,-64.366767 -63.2263674,-64.366767 -62.9537037,-64.366767 -62.68104))", "dataset_titles": "Information on 2023 collection sites for Belgica antarctica; LMG2002 Expedtition Data; Long-term recovery from freezing in Belgica antarctica; Simulated winter warming negatively impacts survival of Antarctica\u0027s only endemic insect", "datasets": [{"dataset_uid": "200222", "doi": "10.7284/908802", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "LMG2002 Expedtition Data", "url": "https://www.rvdata.us/search/cruise/LMG2002"}, {"dataset_uid": "601687", "doi": "10.15784/601687", "keywords": "Antarctica; Antarctic Peninsula; Belgica Antarctica; Biota; Sample Location", "people": "Kawarasaki, Yuta; Lima, Cleverson; Peter, Convey; Michel, Andrew; Teets, Nicholas; Pavinato, Vitor; Gantz, Joseph; Devlin, Jack", "repository": "USAP-DC", "science_program": null, "title": "Information on 2023 collection sites for Belgica antarctica", "url": "https://www.usap-dc.org/view/dataset/601687"}, {"dataset_uid": "601694", "doi": null, "keywords": "Antarctica", "people": "Kawarasaki, Yuta; Devlin, Jack; Unfried, Laura; McCabe, Eleanor; Gantz, Josiah D.; Elnitsky, Michael; Hotaling, Scott; Michel, Andrew; Convey, Peter; Hayward, Scott; Teets, Nicholas", "repository": "USAP-DC", "science_program": null, "title": "Simulated winter warming negatively impacts survival of Antarctica\u0027s only endemic insect", "url": "https://www.usap-dc.org/view/dataset/601694"}, {"dataset_uid": "601698", "doi": "10.15784/601698", "keywords": "Antarctica; Belgica Antarctica; Palmer Station", "people": "Devlin, Jack; Teets, Nicholas; Lima, Cleverson; Lecheta, Melise", "repository": "USAP-DC", "science_program": null, "title": "Long-term recovery from freezing in Belgica antarctica", "url": "https://www.usap-dc.org/view/dataset/601698"}], "date_created": "Fri, 25 Jun 2021 00:00:00 GMT", "description": "The cold, dry terrestrial environments of Antarctica are inhospitable for insects, and only three midge species make Antarctica home. Of these, Belgica antarctica is the only species found exclusively in Antarctica, and it has been a resident of Antarctica since the continent split from South America ~30 million years ago. Thus, this species is an excellent system to model the biological history of Antarctica throughout its repeated glaciation events and shifts in climate. This insect is also a classic example of extreme adaptation, and much previous work has focused on identifying the genetic and physiological mechanisms that allow this species to survive where no other insect is capable. However, it has been difficult to pinpoint the unique evolutionary adaptations that are required to survive in Antarctica due to a lack of information from closely related Antarctic and sub-Antarctic species. This project will compare adaptations, genome sequences, and population characteristics of four midge species that span an environmental gradient from sub-Antarctic to Antarctic habitats. In addition to B. antarctica, these species include two species that are strictly sub-Antarctic and a third that is native to the sub-Antarctic but has invaded parts of Antarctica. The researchers, comprised of scientists from the US, UK, Chile, and France, will sample insects from across their geographic range and measure their ability to tolerate environmental stressors (i.e., cold and desiccation), quantify molecular responses to stress, and compare the makeup of the genome and patterns of genetic diversity. This research will contribute to a greater understanding of adaptation to extremes, to an understanding of biodiversity on the planet and to understanding and predicting changes accompanying environmental change. The project will train two graduate students and two postdoctoral researchers, and a K-12 educator will be a member of the field team and will assist with fieldwork and facilitate outreach with schools in the US. The project includes partnership activities with several STEM education organizations to deliver educational content to K-12 and secondary students. This is a project that is jointly funded by the National Science Foundation\u0027s Directorate of Geosciences (NSF/GEO) and the National Environment Research Council (NERC) of the United Kingdom (UK) via the NSF/GEO-NERC Lead Agency Agreement. This Agreement allows a single joint US/UK proposal to be submitted and peer-reviewed by the Agency whose investigator has the largest proportion of the budget. Each Agency funds the proportion of the budget and the investigators associated with its own country. UK participation in this project includes deploying scientists as part of the field team, supporting field and sampling logistics at remote Antarctic sites, and genome sequencing, annotation, and analyses.\r\n\r\nThis project focuses on the key physiological adaptations and molecular processes that allow a select few insect species to survive in Antarctica. The focal species are all wingless with limited dispersal capacity, suggesting there is also significant potential to locally adapt to variable environmental conditions across the range of these species. The central hypothesis is that similar molecular mechanisms drive both population-level adaptation to local environmental conditions and macroevolutionary changes across species living in different environments. The specific aims of the project are to 1) Characterize conserved and species-specific adaptations to extreme environments through comparative physiology and transcriptomics, 2) Compare the genome sequences of these species to identify genetic signatures of extreme adaption, and 3) Investigate patterns of diversification and local adaptation across each species? range using population genomics. The project establishes an international collaboration of researchers from the US, UK, Chile, and France with shared interests and complementary expertise in the biology, genomics, and conservation of Antarctic arthropods. The Broader Impacts of the project include training students and partnering with the Living Arts and Science Center to design and implement educational content for K-12 students.\r\n\r\nThis award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": -60.616133, "geometry": "POINT(-62.49145 -64.0443585)", "instruments": null, "is_usap_dc": true, "keywords": "Antarctic Peninsula; Livingston Island; Antarctica; USAP-DC; AMD; R/V LMG; USA/NSF; ARTHROPODS; Amd/Us; Anvers Island", "locations": "Antarctica; Antarctic Peninsula; Anvers Island; Livingston Island", "north": -62.68104, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Teets, Nicholas; Michel, Andrew", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "R2R", "repositories": "R2R; USAP-DC", "science_programs": null, "south": -65.407677, "title": "NSFGEO-NERC: Mechanisms of Adaptation to Terrestrial Antarctica through Comparative Physiology and Genomics of Antarctic and sub-Antarctic Insects", "uid": "p0010203", "west": -64.366767}, {"awards": "1906015 Kelley, Joanna", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "Data, Code, and Results for the Zoarcoidei Phylogeny (Hotaling et al.)", "datasets": [{"dataset_uid": "200221", "doi": "10.5281/zenodo.4306092).", "keywords": null, "people": null, "repository": "Zenodo", "science_program": null, "title": "Data, Code, and Results for the Zoarcoidei Phylogeny (Hotaling et al.)", "url": "https://doi.org/10.5281/zenodo.4306092"}], "date_created": "Fri, 25 Jun 2021 00:00:00 GMT", "description": "Fish that reside in the harsh, subfreezing waters of the Antarctic and Arctic provide fascinating examples of adaptation to extreme environments. Species at both poles have independently evolved ways to deal with constant cold temperature, including the evolution of antifreeze proteins. Under freezing conditions, these compounds attach to ice crystals and prevent their growth. This lowers the tissue freezing point and reduces the chance the animal will be injured or killed. While it might seem that the need for unique adaptations to survive in polar waters would reduce species diversity in these habitats, recent evidence showed higher speciation rates in fishes from polar environments as compared to those found in warmer waters. This is despite the fact cold temperatures slow cellular processes, which had been expected to lower rates of molecular evolution in these species. To determine how rates of speciation and molecular evolution are linked in marine fishes, this project will compare the genomes of multiple polar and non-polar fishes. By doing so, it will (1) clarify how rates of evolution vary in polar environments, (2) identify general trends that shape the adaptive trajectories of polar fishes, and (3) determine how functional differences shape the evolution of novel compounds such as the antifreeze proteins some polar fishes rely upon to survive. In addition to training a new generation of scientists, the project will develop curriculum and outreach activities for elementary and undergraduate science courses. Materials will be delivered in classrooms across the western United States, with a focus on rural schools as part of a network for promoting evolutionary education in rural communities.\r\n\r\nTo better understand the biology of polar fishes and the evolution of antifreeze proteins (AFPs), this research will compare the evolutionary histories of cold-adapted organisms to those of related non-polar species from both a genotypic and phenotypic context. In doing so, this research will test whether evolutionary rates are slowed in polar environments, perhaps due to constraints on cellular processes. It will also evaluate the effects of positive selection and the relaxation of selection on genes and pathways, both of which appear to be key adaptive strategies involved in the adaptation to polar environments. To address specific mechanisms by which extreme adaptation occurs, researchers will determine how global gradients of temperature and dissolved oxygen shape genome variation and influence adaptive trajectories among multiple species of eelpouts (family Zoarcidae). An in-vitro experimental approach will then be used to test functional hypotheses about the role of copy number variation in AFP evolution, and how and why multiple antifreeze protein isoforms have evolved. By comparing the genomes of multiple polar and non-polar fishes, the project will clarify how rates of evolution vary in polar environments, identify general trends that shape the adaptive trajectories of cold-adapted marine fishes, and determine how functional differences shape the evolution of novel proteins. This project addresses the strategic programmatic aim to provide a better understanding of the genetic underpinnings of organismal adaptations to their current environment and ways in which polar fishes may respond to changing conditions over different evolutionary time scales. The project is jointly funded by the Antarctic Organisms and Ecosystems Program in the Office of Polar Programs of the Geosciences Directorate, and the Molecular Biophysics Program of the Division of Molecular and Cellular Biosciences in the Biological Sciences Directorate.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "Antarctica; FISH; MARINE ECOSYSTEMS; LABORATORY; AMD; USAP-DC; Amd/Us; USA/NSF", "locations": "Antarctica", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Kelley, Joanna", "platforms": "OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repo": "Zenodo", "repositories": "Zenodo", "science_programs": null, "south": -90.0, "title": "Genome Evolution in Polar Fishes", "uid": "p0010200", "west": -180.0}, {"awards": "1543450 Countway, Peter", "bounds_geometry": "POLYGON((-66 -63,-65.7 -63,-65.4 -63,-65.1 -63,-64.8 -63,-64.5 -63,-64.2 -63,-63.9 -63,-63.6 -63,-63.3 -63,-63 -63,-63 -63.3,-63 -63.6,-63 -63.9,-63 -64.2,-63 -64.5,-63 -64.8,-63 -65.1,-63 -65.4,-63 -65.7,-63 -66,-63.3 -66,-63.6 -66,-63.9 -66,-64.2 -66,-64.5 -66,-64.8 -66,-65.1 -66,-65.4 -66,-65.7 -66,-66 -66,-66 -65.7,-66 -65.4,-66 -65.1,-66 -64.8,-66 -64.5,-66 -64.2,-66 -63.9,-66 -63.6,-66 -63.3,-66 -63))", "dataset_titles": "Biogenic Sulfur Samples from Station E (Palmer Station, Antarctica) and Associated Incubation Experiments; Dissolved Inorganic Nutrient Samples from Station E (Palmer Station, Antarctica) and Associated Incubation Experiments ; Dissolved Organic Carbon (DOC) and Total Dissolved Nitrogen (TDN) Samples from Station E (Palmer Station, Antarctica) and Associated Incubation Experiments; Flow Cytometry Samples from Station E (Palmer Station, Antarctica) and Associated Incubation Experiments; Heterotrophic Bacterial Production Samples from Station E (Palmer Station, Antarctica) and Associated Incubation Experiments; Western Antarctic Peninsula plankton raw sequence reads", "datasets": [{"dataset_uid": "601648", "doi": "10.15784/601648", "keywords": "Antarctica; Biota; Dimethyl Sulfide; Dimethylsulfoniopropionate; Dimethylsulfoxide; DMSP; DMSP Lyase; Palmer Station", "people": "Countway, Peter; Matrai, Patricia", "repository": "USAP-DC", "science_program": null, "title": "Biogenic Sulfur Samples from Station E (Palmer Station, Antarctica) and Associated Incubation Experiments", "url": "https://www.usap-dc.org/view/dataset/601648"}, {"dataset_uid": "601647", "doi": "10.15784/601647", "keywords": "Antarctica; Palmer Station; Phytoplankton", "people": "Matrai, Patricia; Countway, Peter", "repository": "USAP-DC", "science_program": null, "title": "Flow Cytometry Samples from Station E (Palmer Station, Antarctica) and Associated Incubation Experiments", "url": "https://www.usap-dc.org/view/dataset/601647"}, {"dataset_uid": "601646", "doi": "10.15784/601646", "keywords": "Antarctica; Carbon; Dissolved Organic Carbon; Nitrogen; Palmer Station; TDN; Total Dissolved Nitrogen", "people": "Matrai, Patricia; Countway, Peter", "repository": "USAP-DC", "science_program": null, "title": "Dissolved Organic Carbon (DOC) and Total Dissolved Nitrogen (TDN) Samples from Station E (Palmer Station, Antarctica) and Associated Incubation Experiments", "url": "https://www.usap-dc.org/view/dataset/601646"}, {"dataset_uid": "601645", "doi": "10.15784/601645", "keywords": "Antarctica; Nitrate; Nitrite; Palmer Station; Phosphate", "people": "Matrai, Patricia; Countway, Peter", "repository": "USAP-DC", "science_program": null, "title": "Dissolved Inorganic Nutrient Samples from Station E (Palmer Station, Antarctica) and Associated Incubation Experiments ", "url": "https://www.usap-dc.org/view/dataset/601645"}, {"dataset_uid": "601644", "doi": "10.15784/601644", "keywords": "3H-Leu; Antarctica; Bacteria; Biota; DMSP; Heterotrophic Bacterial Production; Palmer Station", "people": "Matrai, Patricia; Countway, Peter", "repository": "USAP-DC", "science_program": null, "title": "Heterotrophic Bacterial Production Samples from Station E (Palmer Station, Antarctica) and Associated Incubation Experiments", "url": "https://www.usap-dc.org/view/dataset/601644"}, {"dataset_uid": "200337", "doi": "", "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "Western Antarctic Peninsula plankton raw sequence reads", "url": "https://dataview.ncbi.nlm.nih.gov/object/PRJNA870587?reviewer=bmud2tbbrqbus79i2n2hb83uio"}], "date_created": "Sat, 01 Aug 2020 00:00:00 GMT", "description": "The Southern Ocean in the vicinity of Antarctica is a region characterized by seasonally-driven marine phytoplankton blooms that are often dominated by microalgal species which produce large amounts of dimethylsulfoniopropionate (DMSP). DMSP can be converted to the compound dimethylsulfide (DMS) which is a molecule that can escape into the atmosphere where it is known to have strong condensation properties that are involved in regional cloud formation. Production of DMSP can influence the diversity and composition of microbial assemblages in seawater and the types and activities of microbes in the seawater will likely affect the magnitude of DMSP\\DMS production. The project examined the role of DMSP in structuring the microbial communities in Antarctic waters and how this structuring may influence DMSP cycling. The project interacted with elementary students in Maine and brought undergraduate students to Bigelow Laboratory. The project also engaged with a science writer and illustrator who joined the team in Palmer Station in 2018. Many posts are available at xxx\r\n\r\nThe project is examining (1) the extent to which the cycling of DMSP in southern ocean waters influenced the composition and diversity of bacterial and protistan assemblages; (2) conversely, whether the composition and diversity of southern ocean protistan and bacterial assemblages influenced the magnitude and rates of DMSP cycling; we are awaiting results on (3) the expression of DMSP degradation genes by marine bacteria seasonally and in response to field experimental additions of DMSP; and, this year (2020-21), we will synthesize these results by quantifying (4) the microbial networks resulting from the presence of DMSP-producers and DMSP-consumers along with their predators, all involved in the cycling of DMSP in southern ocean waters. The work was accomplished by conducting continuous growth experiments with DMSP-amended natural samples of different microbial communities present in summer (2016-17) and fall (2018) at Palmer Station, WAP. Data from the molecular (such as 16S/ 18S tag sequences, DMSP-cycle gene transcripts) and biogeochemical (such as biogenic sulfur cycling, bacterial production, microbial biomass) investigations will be integrated via network analysis in the coming year (2020-21). ", "east": -63.0, "geometry": "POINT(-64.5 -64.5)", "instruments": null, "is_usap_dc": true, "keywords": "USAP-DC; COMMUNITY DYNAMICS; FIELD INVESTIGATION; AMD; PLANKTON; Amd/Us; BIOGEOCHEMICAL CYCLES; Palmer Station; USA/NSF", "locations": "Palmer Station", "north": -63.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Countway, Peter", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION", "repo": "USAP-DC", "repositories": "GenBank; USAP-DC", "science_programs": null, "south": -66.0, "title": "Microbial Community Structure and Expression of Functional Genes Involved in the Seasonal Cycling of DMSP in the Southern Ocean", "uid": "p0010120", "west": -66.0}, {"awards": "1543328 Van Mooy, Benjamin", "bounds_geometry": null, "dataset_titles": "Lipidomics of Antarctic waters. (TBD)", "datasets": [{"dataset_uid": "200149", "doi": "TBD", "keywords": null, "people": null, "repository": "BCO-DMO", "science_program": null, "title": "Lipidomics of Antarctic waters. (TBD)", "url": "https://www.bco-dmo.org/data"}], "date_created": "Fri, 19 Jun 2020 00:00:00 GMT", "description": "The depletion of stratospheric ozone over Antarctica leads to abnormally high levels of ultraviolet radiation (UVR) from the sun reaching the surface of the ocean. This phenomenon is predicted to continue for the next half century, despite bans on ozone-destroying pollutants. Phytoplankton in the near surface ocean are subjected to variable amounts of UVR and contain a lot of lipids (fats). Because phytoplankton are at the base of the food chain their lipids makes their way into the Antarctic marine ecosystem\u0027s food web. The molecular structures of phytoplankton lipids are easily altered by UVR. When this happens, their lipids can be transformed from healthy molecules into potentially harmful molecules(oxylipins) known to be disruptive to reproductive and developmental processes. This project will use state-of-the-art molecular methods to answer questions about extent to which UVR damages lipid molecules in phytoplankton, and how these resultant molecules might effect the food chain in the ocean near Antarctica. \u003cbr/\u003e\u003cbr/\u003e\u003cbr/\u003eLipid peroxidation is often invoked as consequence of increased exposure of phytoplankton to UVR-produced reactive oxygen species (ROS), but the literature is practically silent on peroxidized lipids and their byproducts (i.e. oxylipins) in the ocean. In waters of the West Antarctic Peninsula (WAP), spring-time blooms of diatoms contribute significantly to overall marine primary production. Oxylipins from diatoms can be highly bioactive; their impact on zooplankton grazers, bacteria, and other phytoplankton has been the subject of intense study. However, almost all of this work has focused on the production of oxylipins via enzymatic pathways, not by pathways involving UVR and/or ROS. Furthermore, rigorous experimental work on the effects of oxylipins has been confined almost exclusively to pure cultures and artificial communities. Thus, the true potential of these molecules to disrupt carbon cycling is very poorly-constrained, and is entirely unknown in the waters of the WAP. Armed with new highly-sensitive, state-of-the-art analytical techniques based on high-mass-resolution mass spectrometry, the principal investigator and his research group have begun to uncover an exquisite diversity of oxylipins in natural WAP planktonic communities. These techniques will be applied to understand the connections between UVR, ROS, oxylipins, and carbon cycling. The project will answer the question of how UVR, via ROS, affects oxylipin production by diatoms in WAP surface waters in controlled experiments conducted at a field station. With the answer to this question in hand, the project will also seek to answer how this phenomenon impacts the flow of carbon, particularly the export of organic carbon from the system, during a research cruise. The level of UVR-induced stresses experienced by oxylipin-rich planktonic communities in the WAP is unique, making Antarctica the only location for answering these fundamental questions. Major activities will include laboratory experiments with artificial membranes and diatom cultures, as well field experiments with phytoplankton, zooplankton, and bacteria in WAP waters.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "Oxylipins; Palmer Station; UV Radiation; USAP-DC; West Antarctic Shelf; NOT APPLICABLE; AQUATIC SCIENCES; Phytoplankton", "locations": "West Antarctic Shelf; Palmer Station", "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Van Mooy, Benjamin", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "BCO-DMO", "repositories": "BCO-DMO", "science_programs": null, "south": null, "title": "Production and Fate of Oxylipins in Waters of the Western Antarctic Peninsula: Linkages Between UV Radiation, Lipid Peroxidation, and Carbon Cycling", "uid": "p0010109", "west": null}, {"awards": "1907974 Saltzman, Eric", "bounds_geometry": "POLYGON((129.26 -89.86,130.261 -89.86,131.262 -89.86,132.263 -89.86,133.264 -89.86,134.265 -89.86,135.266 -89.86,136.267 -89.86,137.268 -89.86,138.269 -89.86,139.27 -89.86,139.27 -89.861,139.27 -89.862,139.27 -89.863,139.27 -89.864,139.27 -89.865,139.27 -89.866,139.27 -89.867,139.27 -89.868,139.27 -89.869,139.27 -89.87,138.269 -89.87,137.268 -89.87,136.267 -89.87,135.266 -89.87,134.265 -89.87,133.264 -89.87,132.263 -89.87,131.262 -89.87,130.261 -89.87,129.26 -89.87,129.26 -89.869,129.26 -89.868,129.26 -89.867,129.26 -89.866,129.26 -89.865,129.26 -89.864,129.26 -89.863,129.26 -89.862,129.26 -89.861,129.26 -89.86))", "dataset_titles": "H2 in South Pole firn air", "datasets": [{"dataset_uid": "601332", "doi": "10.15784/601332", "keywords": "Antarctica; Firn; Glaciology; Hydrogen; Ice Core Records; Snow/ice; Snow/Ice; South Pole", "people": "Saltzman, Eric", "repository": "USAP-DC", "science_program": null, "title": "H2 in South Pole firn air", "url": "https://www.usap-dc.org/view/dataset/601332"}], "date_created": "Tue, 09 Jun 2020 00:00:00 GMT", "description": "Hydrogen (H2) is one of the most abundant trace gases in the atmosphere, with a mean level of 500 ppb and an atmospheric lifetime of about two years. Hydrogen has an impact on both air quality and climate, due to its role as a precursor for tropospheric ozone and stratospheric water vapor. Projections indicate that a future \"hydrogen economy\" would increase hydrogen emissions. Understanding of the atmospheric hydrogen budget is largely based on a 30-year record of surface air measurements, but there are no long-term records with which to assess either: 1) the influence of climate change on atmospheric hydrogen, or 2) the extent to which humans have impacted the hydrogen budget. Polar ice core records of hydrogen will advance our understanding of the atmospheric hydrogen cycle and provide a stronger basis for projecting future changes to atmospheric levels of hydrogen and their impacts. \u003cbr/\u003e\u003cbr/\u003eThe research will involve laboratory work to enable the collection and analysis of hydrogen in polar ice cores. Hydrogen is a highly diffusive molecule and, unlike most other atmospheric gases, diffusion of hydrogen in ice is so rapid that ice samples must be stored in impermeable containers immediately upon drilling and recovery. This project will: 1) construct a laboratory system for extracting and analyzing hydrogen in polar ice, 2) develop and test materials and construction designs for vessels to store ice core samples in the field, and 3) test the method on samples of opportunity previously stored in the field. The goal of this project is a proven, cost-effective design for storage flasks to be fabricated for use on future polar ice coring projects. This project will support the dissertation research of a graduate student in the UC Irvine Department of Earth System Science.\u003cbr/\u003e\u003cbr/\u003eThis award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 139.27, "geometry": "POINT(134.265 -89.865)", "instruments": null, "is_usap_dc": true, "keywords": "LABORATORY; Firn; TRACE GASES/TRACE SPECIES; South Pole; FIELD INVESTIGATION; USAP-DC", "locations": "South Pole", "north": -89.86, "nsf_funding_programs": "Antarctic Instrumentation and Facilities; Antarctic Glaciology; Antarctic Instrumentation and Support", "paleo_time": null, "persons": "Saltzman, Eric", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION; OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -89.87, "title": "EAGER: Feasibility of Reconstructing the Atmospheric History of Molecular Hydrogen from Antarctic Ice", "uid": "p0010106", "west": 129.26}, {"awards": "1543419 Place, Sean", "bounds_geometry": "POLYGON((163 -76,163.5 -76,164 -76,164.5 -76,165 -76,165.5 -76,166 -76,166.5 -76,167 -76,167.5 -76,168 -76,168 -76.2,168 -76.4,168 -76.6,168 -76.8,168 -77,168 -77.2,168 -77.4,168 -77.6,168 -77.8,168 -78,167.5 -78,167 -78,166.5 -78,166 -78,165.5 -78,165 -78,164.5 -78,164 -78,163.5 -78,163 -78,163 -77.8,163 -77.6,163 -77.4,163 -77.2,163 -77,163 -76.8,163 -76.6,163 -76.4,163 -76.2,163 -76))", "dataset_titles": "Hsc71 MK948005; Hsc71 MN045883; Hsp40 MK948006; Hsp40 MN045884; Hsp90ab MK948004; Hsp90aB MN045885; Hsp90b MN367227; Hspa12a MN367222; Hspa12a MN367224; Hspa13 MN367223; Hspa13 MN367225; Hspa4 MK948003; Hspa4 MN045886; Hspa6 MN367221; HspA6 MN367226; miRNA Characterization in Antarctic fish", "datasets": [{"dataset_uid": "200058", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Hspa4 MK948003", "url": ""}, {"dataset_uid": "200066", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Hsc71 MN045883", "url": ""}, {"dataset_uid": "200065", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Hspa13 MN367223", "url": ""}, {"dataset_uid": "200064", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Hspa12a MN367222", "url": ""}, {"dataset_uid": "200063", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Hspa6 MN367221", "url": ""}, {"dataset_uid": "200062", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Hsp40 MK948006", "url": ""}, {"dataset_uid": "200061", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Hsc71 MK948005", "url": ""}, {"dataset_uid": "200060", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Hsp90ab MK948004", "url": ""}, {"dataset_uid": "200059", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Hspa4 MK948003", "url": ""}, {"dataset_uid": "200057", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "miRNA Characterization in Antarctic fish", "url": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE128132"}, {"dataset_uid": "200073", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Hsp90b MN367227", "url": ""}, {"dataset_uid": "200072", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "HspA6 MN367226", "url": ""}, {"dataset_uid": "200071", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Hspa13 MN367225", "url": ""}, {"dataset_uid": "200070", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Hspa12a MN367224", "url": ""}, {"dataset_uid": "200069", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Hspa4 MN045886", "url": ""}, {"dataset_uid": "200068", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Hsp90aB MN045885", "url": ""}, {"dataset_uid": "200067", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Hsp40 MN045884", "url": ""}], "date_created": "Thu, 19 Sep 2019 00:00:00 GMT", "description": "The project will integrate analyses of fish physiology, protein production and genetics to determine if regulation of molecular chaperones (a class of proteins that facilitate the proper folding of proteins in a cell) has been permanently lost in a key fish species (Trematomus bernacchii) inhabiting the Southern Ocean. To do so, efforts will be undertaken to analyze chaperones in these fishes and how elevated temperatures impact protein turnover and protein damage. These studies should more definitively determine if the interruption of chaperone function is environmentally controlled (which could suggest these fish could benefit in some form by increasing sea surface temperatures) or if there is complete loss of chaperone function due to a change in its structure through evolutionary processes (which would suggest these fish are less likely to be able to adapt to warming). In addition to filling key gaps in our knowledge about the diversity and evolution of fishes in the southern ocean and the potential impacts changing temperatures might have on fish populations, the project will support the training of undergraduate and graduate students at an RUI institution. Specifically, activities and content directly related to this project\u0027s aims will be incorporated into the undergraduate curriculum at Sonoma State University in an effort to increase undergraduate participation in research, especially with respect to underrepresented groups.\u003cbr/\u003e\u003cbr/\u003eThe project has specific aims to perform a comparative analysis of nucleotide divergence resulting in non-synonymous amino acid changes in the trans-regulatory elements, namely members of the heat shock factor (HSF) family of transcription factors, in T. bernacchii and N. angustata. The project will also utilize metabolic labeling of newly synthesized proteins from isolated hepatocytes to monitor protein turnover rates in fish acclimated to both -1.5 and +4 \u00b0C for an extended period. Changes in chaperoning capacity and levels of damaged proteins will be quantified in multiple tissues to gain a better understanding of the cellular requirements for maintaining protein homeostasis under long-term acclimations to +4 \u00b0C. In combination, the work will help answer questions regarding divergence in these fishes as well a fundamental information regarding protein structure and function that may also have bio-medical implications.", "east": 168.0, "geometry": "POINT(165.5 -77)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "USAP-DC; FISH; NOT APPLICABLE; Southern Ocean", "locations": "Southern Ocean", "north": -76.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Place, Sean", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "NCBI GenBank", "repositories": "NCBI GenBank", "science_programs": null, "south": -78.0, "title": "RUI: Characterizing Protein Homeostasis and the Regulatory Mechanisms Controlling Molecular Chaperone Expression in the Highly Stenothermal Notothenioid Fish, Trematomus Bernacchii", "uid": "p0010055", "west": 163.0}, {"awards": "1341385 Lee, Richard; 1341393 Denlinger, David", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "Alaskozetes antarcticus Raw sequence reads; Belgica antarctica Integrated Genome and Transcriptome Project; Data from: Rapid cold hardening protects against sublethal freezing injury in an Antarctic insect", "datasets": [{"dataset_uid": "200054", "doi": " https://doi.org/10.5061/dryad.29p7ng2", "keywords": null, "people": null, "repository": "Dryad", "science_program": null, "title": "Data from: Rapid cold hardening protects against sublethal freezing injury in an Antarctic insect", "url": "https://datadryad.org/resource/doi:10.5061/dryad.29p7ng2"}, {"dataset_uid": "200052", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Alaskozetes antarcticus Raw sequence reads", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA428758"}, {"dataset_uid": "200053", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Belgica antarctica Integrated Genome and Transcriptome Project", "url": "https://www.ncbi.nlm.nih.gov/bioproject/175916"}], "date_created": "Mon, 12 Aug 2019 00:00:00 GMT", "description": "Polar regions are deserts that are not only cold but also lack access to free water. Antarctic insects have unique survival mechanisms including the ability to tolerate freezing and extensive dehydration, surviving the loss of 70% of their body water. How this is done is of interest not only for understanding seasonal adaptations of insects and how they respond to climate change, but the molecular and physiological mechanisms employed may offer valuable insights into more general mechanisms that might be exploited for cryopreservation and long-term storage of human tissues and organs for transplantation and other medical applications. The investigators will study the proteins that are responsible for removing water from the body, cell level consequences of this, and how the responsible genes vary between populations. The project will also further the NSF goals of making scientific discoveries available to the general public and of training new generations of scientists. Each year a K-12 teacher will be a member of the field team and assist with fieldwork and outreach to school children and their teachers. Educational outreach efforts include presentations at local schools and national teacher meetings, providing lesson plans and podcasts on a website, and continuing to publish articles related to this research in education journals. In addition, undergraduate and graduate students will receive extensive training in all aspects of the research project with extended experiences that include publication of scientific papers and presentations at national meetings.\u003cbr/\u003e\u003cbr/\u003eThis project focuses on deciphering the physiological and molecular mechanisms that enable the Antarctic midge Belgica antarctica to survive environmental stress and the loss of most of its body water in the desiccating polar environment. This extremophile is an ideal system for investigating mechanisms of stress tolerance and local geographic adaptations and its genome has recently been sequenced. This project has three focal areas: 1) Evaluating the role of aquaporins (water channel proteins) in the rapid removal of water from the body by studying expression of their genes during dehydration; 2) Investigating the mechanism of metabolic depression and the role of autophagy (controlled breakdown of cellular components) as a mediator of stress tolerance by studying expression of the genes responsible for autophagy during the dehydration process; and 3) Evaluating the population structure, gene flow, and adaptive variation in physiological traits associated with stress tolerance using a genetic approach that takes advantage of the genomic sequence available for this species coupled with physiological and environmental data from the sampled populations and their habitats.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "Antarctica; USAP-DC; ARTHROPODS; NOT APPLICABLE", "locations": "Antarctica", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Denlinger, David; Lee, Richard", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "Dryad", "repositories": "Dryad; NCBI GenBank", "science_programs": null, "south": -90.0, "title": "Collaborative Research: Winter Survival Mechanisms and Adaptive Genetic Variation in an Antarctic Insect", "uid": "p0010048", "west": -180.0}, {"awards": "1745036 Marchetti, Adrian; 1744760 Hopkinson, Brian", "bounds_geometry": "POLYGON((-77 -61,-75.2 -61,-73.4 -61,-71.6 -61,-69.8 -61,-68 -61,-66.2 -61,-64.4 -61,-62.6 -61,-60.8 -61,-59 -61,-59 -62.1,-59 -63.2,-59 -64.3,-59 -65.4,-59 -66.5,-59 -67.6,-59 -68.7,-59 -69.8,-59 -70.9,-59 -72,-60.8 -72,-62.6 -72,-64.4 -72,-66.2 -72,-68 -72,-69.8 -72,-71.6 -72,-73.4 -72,-75.2 -72,-77 -72,-77 -70.9,-77 -69.8,-77 -68.7,-77 -67.6,-77 -66.5,-77 -65.4,-77 -64.3,-77 -63.2,-77 -62.1,-77 -61))", "dataset_titles": "Photosynthetic physiological data of Proteorhodopsin containing diatoms under differing iron availabilities", "datasets": [{"dataset_uid": "601530", "doi": "10.15784/601530", "keywords": "Antarctica; Diatom", "people": "Hopkinson, Brian; Andrew, Sarah; Marchetti, Adrian; Plumb, Kaylie", "repository": "USAP-DC", "science_program": null, "title": "Photosynthetic physiological data of Proteorhodopsin containing diatoms under differing iron availabilities", "url": "https://www.usap-dc.org/view/dataset/601530"}], "date_created": "Sun, 16 Jun 2019 00:00:00 GMT", "description": "Proteorhodopsins (PR) are retinal-binding membrane proteins that can act as light-driven proton pumps to generate energy that can be used for metabolism and growth. The discovery of PRs in many diverse marine prokaryotic microbes has initiated extensive investigations into their distributions and functional roles. Recently, a rhodopsin-like gene of the proton-pumping variety was identified in diatoms thus revealing their presence within obligate marine eukaryotic photoautotrophs. Since this time, PRs have been identified in a number of diatom isolates although there appears to be a much higher frequency of\r\nPR in diatoms residing in cold, iron-limited regions of the ocean, particularly in the Southern Ocean (SO). PR is especially suited for use in SO phytoplankton since unlike conventional photosynthesis, it uses no iron and its reaction rate is insensitive to temperature. The overall objective of our proposed project is to characterize Antarctic diatom-PR and determine its role in the adaptation of SO diatoms to the prevailing conditions of low iron concentrations and extremely low temperatures. Our research objectives will be achieved through a combination of molecular, biochemical and physiological measurements in diatom isolates recently obtained from the Western Antarctic Peninsula region. We will determine the proton-pumping characteristics and pumping rates of PR as a function of light intensity and wavelength, the resultant PR-linked intracellular ATP production rates, and the cellular localization of the protein. We will examine under which environmental conditions Antarctic diatom-PR is most highly expressed and construct a cellular energy budget that includes diatom-PR when grown under these different growth conditions. Estimates of the energy flux generated by PR in PR-containing diatoms will be compared to total energy generation by the photosynthetic light reactions and metabolically coupled respiration rates. Finally, we will compare the characteristics and gene expression of diatom-PR in Antarctic diatoms to PR-containing diatoms isolated from temperate regions in order to investigate if there is a preferential dependence on energy production through diatom-PR in diatoms residing in cold, iron-limited regions of the ocean.", "east": -59.0, "geometry": "POINT(-68 -66.5)", "instruments": null, "is_usap_dc": true, "keywords": "FIELD INVESTIGATION; NSF/USA; Southern Ocean; AMD; Amd/Us; LABORATORY; USAP-DC; BIOGEOCHEMICAL CYCLES", "locations": "Southern Ocean", "north": -61.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Marchetti, Adrian; Septer, Alecia; Hopkinson, Brian", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION; OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -72.0, "title": "Collaborative research: Antarctic diatom proteorhodopsins: Characterization and a potential role in the iron-limitation response", "uid": "p0010033", "west": -77.0}, {"awards": "1642570 Thurber, Andrew", "bounds_geometry": "POINT(166.666 -77.8)", "dataset_titles": "Microbial community composition of the Cinder Cones Cold Seep", "datasets": [{"dataset_uid": "200035", "doi": "DOI:10.1575/1912/bco-dmo.756997.1", "keywords": null, "people": null, "repository": "BCO-DMO", "science_program": null, "title": "Microbial community composition of the Cinder Cones Cold Seep", "url": "https://www.bco-dmo.org/dataset/756997"}], "date_created": "Fri, 24 May 2019 00:00:00 GMT", "description": "Methane is a potent greenhouse gas that is naturally emitted into the oceans by geologic seeps and microbial production. Based on studies of persistent deep-sea seeps at mid- and northern latitudes, researchers have learned that bacteria and archaea can create a \"sediment filter\" that oxidizes methane prior to its release. Antarctica is thought to contain large reservoirs of organic carbon buried beneath its ice which could a quantity of methane equivalent to all of the permafrost in the Arctic and yet we know almost nothing about the methane oxidizing microbes in this region. How these microbial communities develop and potentially respond to fluctuations in methane levels is an under-explored avenue of research. A bacterial mat was recently discovered at 78 degrees south, suggesting the possible presence of a methane seep, and associated microbial communities. This project will explore this environment in detail to assess the levels and origin of methane, and the nature of the microbial ecosystem present. \u003cbr/\u003e \u003cbr/\u003eAn expansive bacterial mat appeared and/or was discovered at 78 degrees south in 2011. This site, near McMurdo Station Antarctica, has been visited since the mid-1960s, but this mat was not observed until 2011. The finding of this site provides an unusual opportunity to study an Antarctic marine benthic habitat with active methane cycling and to examine the dynamics of recruitment and community succession of seep fauna including bacteria, archaea, protists and metazoans. This project will collect the necessary baseline data to facilitate further studies of Antarctic methane cycling. The concentration and source of methane will be determined at this site and at potentially analogous sites in McMurdo Sound. In addition to biogeochemical characterization of the sites, molecular analysis of the microbial community will quantify the time scales on which bacteria and archaea respond to methane input and provide information on rates of community development and succession in the Southern Ocean. Project activities will facilitate the training of at least one graduate student and results will be shared at both local and international levels. A female graduate student will be mentored as part of this project and data collected will form part of her dissertation. Lectures will be given in K-12 classrooms in Oregon to excite students about polar science. National and international audiences will be reached through blogs and presentations at a scientific conference. The PI\u0027s previous blogs have been used by K-12 classrooms as part of their lesson plans and followed in over 65 countries.\u003cbr/\u003e", "east": 166.666, "geometry": "POINT(166.666 -77.8)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "Sea Floor; USAP-DC; Ross Sea; BACTERIA/ARCHAEA; NOT APPLICABLE", "locations": "Ross Sea; Sea Floor", "north": -77.8, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Thurber, Andrew", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "BCO-DMO", "repositories": "BCO-DMO", "science_programs": null, "south": -77.8, "title": "EAGER: Elucidating the Antarctic Methane Cycle at the Cinder Cones Reducing Habitat.", "uid": "p0010030", "west": 166.666}, {"awards": "1247510 Detrich, H. William", "bounds_geometry": null, "dataset_titles": "E-MTAB-6759: RNA-seq across tissues in four Notothenioid species (Antarctic icefish); Expedition Data; Expedition data of LMG1003; Expedition data of LMG1004; PRJNA420419: Chaenocephalus aceratus Genome sequencing; PRJNA66471: Notothenia coriiceps Genome Sequencing Notothenia coriiceps isolate:Sejong01 (black rockcod); S-BSST 132: Assembled Transcriptomes for Berthelot et al. (2018); SRA091269: Notothenia coriiceps RNA Raw Sequence Reads; SRP047484: RAD-tag Sequences of Genetically Mapped Notothenia coriiceps Embryos ; SRP118539: RAD-tag Sequences of Genetically Mapped Chaenocephalus aceratus Embryos", "datasets": [{"dataset_uid": "001509", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0806"}, {"dataset_uid": "002685", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG1004", "url": "https://www.rvdata.us/search/cruise/LMG1004"}, {"dataset_uid": "200145", "doi": "", "keywords": null, "people": null, "repository": "BioStudies", "science_program": null, "title": "S-BSST 132: Assembled Transcriptomes for Berthelot et al. (2018)", "url": "https://www.ebi.ac.uk/biostudies/studies/S-BSST132"}, {"dataset_uid": "002684", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG1003", "url": "https://www.rvdata.us/search/cruise/LMG1003"}, {"dataset_uid": "001508", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0807"}, {"dataset_uid": "200142", "doi": "", "keywords": null, "people": null, "repository": "NCBI BioProject", "science_program": null, "title": "PRJNA66471: Notothenia coriiceps Genome Sequencing Notothenia coriiceps isolate:Sejong01 (black rockcod)", "url": "https://www.ncbi.nlm.nih.gov/bioproject/66471"}, {"dataset_uid": "200026", "doi": "", "keywords": null, "people": null, "repository": "NCBI SRA", "science_program": null, "title": "SRA091269: Notothenia coriiceps RNA Raw Sequence Reads", "url": "https://www.ncbi.nlm.nih.gov/sra/?term=SRA091269"}, {"dataset_uid": "200144", "doi": "", "keywords": null, "people": null, "repository": "Array Express", "science_program": null, "title": "E-MTAB-6759: RNA-seq across tissues in four Notothenioid species (Antarctic icefish)", "url": "https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-6759/"}, {"dataset_uid": "200143", "doi": "", "keywords": null, "people": null, "repository": "NCBI BioProject", "science_program": null, "title": "PRJNA420419: Chaenocephalus aceratus Genome sequencing", "url": "https://www.ncbi.nlm.nih.gov/bioproject/420419"}, {"dataset_uid": "200146", "doi": "", "keywords": null, "people": null, "repository": "NCBI SRA", "science_program": null, "title": "SRP047484: RAD-tag Sequences of Genetically Mapped Notothenia coriiceps Embryos ", "url": "https://www.ncbi.nlm.nih.gov/sra/?term=SRP047484"}, {"dataset_uid": "200093", "doi": "", "keywords": null, "people": null, "repository": "NCBI SRA", "science_program": null, "title": "SRP118539: RAD-tag Sequences of Genetically Mapped Chaenocephalus aceratus Embryos", "url": "https://www.ncbi.nlm.nih.gov/sra/SRP118539 "}], "date_created": "Mon, 08 Apr 2019 00:00:00 GMT", "description": "Since the advent of Antarctic continental glaciation, the opening of the Drake Passage between South America and the Antarctic Peninsula, and the onset of cooling of the Southern Ocean ~40-25 million years ago, evolution of the Antarctic marine biota has been driven by the development of extreme cold temperatures. As circum-Antarctic coastal temperatures declined during this period from ~20\u00b0C to the modern -1.9 to +2.0\u00b0C (reached ~8-10 million years ago), the psychrophilic (cold-loving) ectotherms of the Southern Ocean evolved compensatory molecular, cellular, and physiological traits that enabled them to maintain normal metabolic function at cold temperatures. Today, these organisms are threatened by rapid warming of the Southern Ocean over periods measured in centuries (as much as 5\u00b0C/100 yr), a timeframe so short that re-adaptation and/or acclimatization to the \"new warm\" may not be possible. Thus, the long-term goals of this research project are: 1) to understand the biochemical and physiological capacities of the embryos of Antarctic notothenioid fish to resist or compensate for rapid oceanic warming; and 2) to assess the genetic toolkit available to support the acclimatization and adaptation of Antarctic notothenioid embryos to their warming habitat. The specific aims of this work are: 1) to determine the capacity of the chaperonin complex of notothenioid fishes to assist protein folding at temperatures between -4 and +20\u00b0C; and 2) to evaluate the genetic responses of notothenioid embryos, measured as global differential gene transcription, to temperature challenge, with -1.9\u00b0C as the \"normal\" control and +4 and +10\u00b0C as high temperature insults.\r\nThe physiology of embryonic development of marine stenotherms under future climate change scenarios is an important but understudied problem. This project will provide valuable insights into the capacity of Antarctic fish embryos to acclimatize and adapt to plausible climate change scenarios by examining multiple levels of biological organization, from the biochemical to the organismal. The results should also be broadly applicable to understanding the impact of global warming on marine biota worldwide. The research will also introduce graduate and undergraduate students to state-of-the-art biochemical, cellular, and molecular-biological research relevant to ecological and environmental issues of the Antarctic marine ecosystem.", "east": null, "geometry": null, "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e SAMPLERS \u003e TRAWLS/NETS \u003e BOTTOM TRAWL", "is_usap_dc": false, "keywords": "AQUATIC SCIENCES; R/V LMG; USAP-DC; Southern Ocean", "locations": "Southern Ocean", "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Detrich, H. William", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "R2R", "repositories": "Array Express; BioStudies; NCBI BioProject; NCBI SRA; R2R", "science_programs": null, "south": null, "title": "Protein Folding and Embryogenesis in Antarctic Fishes: A Comparative Approach to Environmental Stress", "uid": "p0010024", "west": null}, {"awards": "1341479 Marchetti, Adrian", "bounds_geometry": "POLYGON((-72.8 -48,-67.12 -48,-61.44 -48,-55.76 -48,-50.08 -48,-44.4 -48,-38.72 -48,-33.04 -48,-27.36 -48,-21.68 -48,-16 -48,-16 -50.02,-16 -52.04,-16 -54.06,-16 -56.08,-16 -58.1,-16 -60.12,-16 -62.14,-16 -64.16,-16 -66.18,-16 -68.2,-21.68 -68.2,-27.36 -68.2,-33.04 -68.2,-38.72 -68.2,-44.4 -68.2,-50.08 -68.2,-55.76 -68.2,-61.44 -68.2,-67.12 -68.2,-72.8 -68.2,-72.8 -66.18,-72.8 -64.16,-72.8 -62.14,-72.8 -60.12,-72.8 -58.1,-72.8 -56.08,-72.8 -54.06,-72.8 -52.04,-72.8 -50.02,-72.8 -48))", "dataset_titles": "16S and 18S Sequence data; Fragilariopsis kerguelensis iron and light transcriptomes; Physiology and transcriptomes of polar isolates; Polar isolate transcriptomes; Sequence data from Ocean Station Papa seawater ; Sequence data RNA-Seq of marine phytoplankton: FeB12", "datasets": [{"dataset_uid": "200018", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Sequence data from Ocean Station Papa seawater ", "url": "https://www.ncbi.nlm.nih.gov/sra/SRP006906"}, {"dataset_uid": "200017", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Sequence data RNA-Seq of marine phytoplankton: FeB12", "url": "https://www.ncbi.nlm.nih.gov/sra/SRP074366"}, {"dataset_uid": "200016", "doi": "", "keywords": null, "people": null, "repository": "iMicrobe", "science_program": null, "title": "Fragilariopsis kerguelensis iron and light transcriptomes", "url": "https://www.imicrobe.us/#/projects/104"}, {"dataset_uid": "200020", "doi": "", "keywords": null, "people": null, "repository": "BCO-DMO", "science_program": null, "title": "Physiology and transcriptomes of polar isolates", "url": "https://www.bco-dmo.org/project/653229"}, {"dataset_uid": "200019", "doi": "", "keywords": null, "people": null, "repository": "Cyverse Data Commons", "science_program": null, "title": "Polar isolate transcriptomes", "url": "http://datacommons.cyverse.org/search/?search_term=unc_phyto_isolates"}, {"dataset_uid": "200021", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "16S and 18S Sequence data", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA299401"}], "date_created": "Mon, 11 Mar 2019 00:00:00 GMT", "description": "The Southern Ocean surrounding Antarctica is changing rapidly in response to Earth\u0027s warming climate. These changes will undoubtedly influence communities of primary producers (the organisms at the base of the food chain, particularly plant-like organisms using sunlight for energy) by altering conditions that influence their growth and composition. Because primary producers such as phytoplankton play an important role in global biogeochemical cycling, it is essential to understand how they will respond to changes in their environment. The growth of phytoplankton in certain regions of the Southern Ocean is constrained by steep gradients in chemical and physical properties that vary in both space and time. Light and iron have been identified as key variables influencing phytoplankton abundance and distribution within Antarctic waters. Microscopic algae known as diatoms are dominant members of the phytoplankton and sea ice communities, accounting for significant proportions of primary production. The overall objective of this project is to identify the molecular bases for the physiological responses of polar diatoms to varying light and iron conditions. The project should provide a means of evaluating the extent these factors regulate diatom growth and influence net community productivity in Antarctic waters. Although numerous studies have investigated how polar diatoms are affected by varying light and iron, the cellular mechanisms leading to their distinct physiological responses remain unknown. We observed several growth responses, but a majority of polar diatom growth rates and photophysiology did not appear to be co-limited by iron and light limitation. Using comparative transcriptomics, we have examined the expression patterns of key genes and metabolic pathways in several ecologically important polar diatoms isolated from Antarctic waters and grown under varying iron and irradiance conditions. In addition, molecular indicators for iron and light limitation will be developed within these polar diatoms through the identification of iron- and light-responsive genes -- the expression patterns of which can be used to determine their physiological status. Upon verification in laboratory cultures, these indicators will be utilized by way of metatranscriptomic sequencing to examine iron and light limitation in natural diatom assemblages collected along environmental gradients in Western Antarctic Peninsula waters. In order to fully understand the role phytoplankton play in Southern Ocean biogeochemical cycles, dependable methods that provide a means of elucidating the physiological status of phytoplankton at any given time and location are essential.", "east": -16.0, "geometry": "POINT(-44.4 -58.1)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "NOT APPLICABLE; AQUATIC SCIENCES; PHYTOPLANKTON; USAP-DC; Southern Ocean; Sea Surface; DIATOMS", "locations": "Sea Surface; Southern Ocean", "north": -48.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Marchetti, Adrian", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "NCBI GenBank", "repositories": "BCO-DMO; Cyverse Data Commons; iMicrobe; NCBI GenBank", "science_programs": null, "south": -68.2, "title": "Iron and Light Limitation in Ecologically Important Polar Diatoms: Comparative Transcriptomics and Development of Molecular Indicators", "uid": "p0010018", "west": -72.8}, {"awards": "1704236 Karentz, Deneb", "bounds_geometry": null, "dataset_titles": "2017 SCAR Biology Symposium travel award data", "datasets": [{"dataset_uid": "601155", "doi": "10.15784/601155", "keywords": "Antarctica; Human Dimensions", "people": "Karentz, Deneb", "repository": "USAP-DC", "science_program": null, "title": "2017 SCAR Biology Symposium travel award data", "url": "https://www.usap-dc.org/view/dataset/601155"}], "date_created": "Mon, 04 Feb 2019 00:00:00 GMT", "description": "This project supported US participation in the XIIth Scientific Committee on Antarctic Research (SCAR) International Biology Symposium. The theme of this meeting and ancillary workshops was Scale Matters. Meeting sessions specifically addressed biodiversity and physiology spanning from molecular through ecosystem scales. \r\n\r\nThe project provided partial support (airfare and meeting registration) for 20 US scientists to travel to Leuven, Belgium and attend the SCAR International Biology Symposium in July 2017. Preference was given to applicants who were students and early career scientists. The call for applications was broadly disseminated to encourage participation by underrepresented groups in the sciences. The SCAR International Biology Symposium is a unique opportunity for US scientists to present their work and learn about the most recent findings on all aspects of Antarctic organisms and ecosystems research, to establish and strengthen international contacts, and to be actively involved in the development of new directions and the establishment of new frontiers in polar biology.", "east": null, "geometry": null, "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "ECOLOGICAL DYNAMICS; North America; NOT APPLICABLE; USAP-DC", "locations": "North America", "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Integrated System Science", "paleo_time": null, "persons": "Karentz, Deneb", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": null, "title": "Group Travel Award: XIIth SCAR International Biology Symposium", "uid": "p0010006", "west": null}, {"awards": "1341612 Bowser, Samuel", "bounds_geometry": null, "dataset_titles": "Aerial survey of Explorers Cove shoreline, late January 2005; Astrammina rara genome sequencing and assembly; Astrammina triangularis genome sequencing and assembly; Crithionina delacai mitochondrial genome sequence and assembly; Scanning electron micrographs: Influence of heavy metal (Pb, Cd) exposure on shell morphogenesis in Astrammina rara, a giant agglutinated Antarctic foraminiferan protist", "datasets": [{"dataset_uid": "200090", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Astrammina rara genome sequencing and assembly", "url": "https://dataview.ncbi.nlm.nih.gov/object/PRJNA521081?reviewer=25e190ih1svottjkrrpfa7huoe"}, {"dataset_uid": "200089", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Astrammina triangularis genome sequencing and assembly", "url": "https://dataview.ncbi.nlm.nih.gov/object/PRJNA521279?reviewer=g418tpq02sif2g6do94dpmmjdv"}, {"dataset_uid": "200091", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Crithionina delacai mitochondrial genome sequence and assembly", "url": "https://dataview.ncbi.nlm.nih.gov/object/PRJNA592714?reviewer=ivse8455h3gfaiilg4nqle0vm1"}, {"dataset_uid": "601138", "doi": "10.15784/601138", "keywords": "Antarctica; Biota; Foraminifera; Heavy Metal Toxicity; Scanning Electron Microscop; Scanning Electron Microscope (SEM) Images; Scanning Electron Microscopy; Transantarctic Mountains", "people": "Bowser, Samuel; Andreas, Amanda", "repository": "USAP-DC", "science_program": null, "title": "Scanning electron micrographs: Influence of heavy metal (Pb, Cd) exposure on shell morphogenesis in Astrammina rara, a giant agglutinated Antarctic foraminiferan protist", "url": "https://www.usap-dc.org/view/dataset/601138"}, {"dataset_uid": "601229", "doi": "10.15784/601229", "keywords": "Aerial Imagery; Antarctica; Camera; Delta; Freshwater; Helicopter; Moat; Shoreline Survey; Small Ponds; Snow Melt; Tide Pools", "people": "Bowser, Samuel; Alexander, Steve", "repository": "USAP-DC", "science_program": null, "title": "Aerial survey of Explorers Cove shoreline, late January 2005", "url": "https://www.usap-dc.org/view/dataset/601229"}], "date_created": "Thu, 29 Nov 2018 00:00:00 GMT", "description": "Agglutinated foraminifera (forams for short) are early-evolving, single-celled organisms. These \"living fossils\" construct protective shells using sediment grains held together by adhesive substances that they secrete. During shell construction, agglutinated forams display amazing properties of selection - for example, some species build their shells of clear quartz grains, while other species use only grains of a specific size. Understanding how these single cells assemble complex structures may contribute to nanotechnology by enabling people to use forams as \"cellular machines\" to aid in the construction of nano-devices. This project will analyze the genomes of at least six key foram species, and then \"mine\" these genomes for technologically useful products and processes. The project will focus initially on the adhesive materials forams secrete, which may have wide application in biomedicine and biotechnology. Furthermore, the work will further develop a molecular toolkit which could open up new avenues of research on the physiology, ecology, and population dynamics of this important group of Antarctic organisms. The project will also further the NSF goals of making scientific discoveries available to the general public and of training new generations of scientists. Educational experiences related to the \"thrill of scientific exploration and discovery\" for students and the general public will be provided through freely-available short films and a traveling art/science exhibition. The project will also provide hands-on research opportunities for undergraduate students.\u003cbr/\u003e\u003cbr/\u003eExplorers Cove, situated on the western shore of McMurdo Sound, harbors a unique population of foraminiferan taxa at depths accessible by scuba diving that otherwise are primarily found in the deep sea. The project will use next-generation DNA sequencing and microdissection methods to obtain and analyze nuclear and mitochondrial genomes from crown members of two species each from three distinct, early-evolving foraminiferal groups. It will also use next generation sequencing methods to characterize the in-situ prokaryotic assemblages (microbiomes) of one of these groups and compare them to reference sediment microbiomes. The phyogenomic studies of the targeted Antarctic genera will help fill significant gaps in our current understanding of early foram evolution. Furthermore, comparative genomic analyses of these six species are expected to yield a better understanding of the physiology of single-chambered agglutinated forams, especially the bioadhesive proteins and regulatory factors involved in shell composition and morphogenesis. Additionally, the molecular basis of cold adaptation in forams will be examined, particularly with respect to key proteins.", "east": null, "geometry": null, "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "USAP-DC; NOT APPLICABLE", "locations": null, "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Bowser, Samuel", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "NCBI GenBank", "repositories": "NCBI GenBank; USAP-DC", "science_programs": null, "south": null, "title": "Assembling and Mining the Genomes of Giant Antarctic Foraminifera", "uid": "p0000004", "west": null}, {"awards": "1144176 Lyons, W. Berry; 1727387 Mikucki, Jill; 1144177 Pettit, Erin; 1144192 Tulaczyk, Slawek", "bounds_geometry": "POLYGON((161.8 -77.7,161.88 -77.7,161.96 -77.7,162.04000000000002 -77.7,162.12 -77.7,162.2 -77.7,162.28 -77.7,162.36 -77.7,162.44 -77.7,162.51999999999998 -77.7,162.6 -77.7,162.6 -77.70700000000001,162.6 -77.714,162.6 -77.721,162.6 -77.728,162.6 -77.735,162.6 -77.742,162.6 -77.749,162.6 -77.756,162.6 -77.76299999999999,162.6 -77.77,162.51999999999998 -77.77,162.44 -77.77,162.36 -77.77,162.28 -77.77,162.2 -77.77,162.12 -77.77,162.04000000000002 -77.77,161.96 -77.77,161.88 -77.77,161.8 -77.77,161.8 -77.76299999999999,161.8 -77.756,161.8 -77.749,161.8 -77.742,161.8 -77.735,161.8 -77.728,161.8 -77.721,161.8 -77.714,161.8 -77.70700000000001,161.8 -77.7))", "dataset_titles": "Ablation Stake Data from of Taylor Glacier near Blood Falls; Antarctica Support 2014/2015 - C-528 Blood Falls GPS/GNSS Observations Dataset; Blood Falls, McMurdo Dry Va. International Federation of Digital Seismograph Networks. Dataset/Seismic Network; FLIR thermal imaging data near Blood Falls, Taylor Glacier; Ground Penetrating Radar Data near Blood Falls, Taylor Glacier; Ice Temperature in Shallow Boreholes Near Blood Falls at the Terminus of Taylor Glacier, McMurdo Dry Valleys, Antarctica; NCBI short read archive -Metagenomic survey of Antarctic Groundwater; Terrestrial Radar Interferometry near Blood Falls, Taylor Glacier; The Geochemistry of englacial brine from Taylor Glacier, Antarctica; Time Lapse imagery of the Blood Falls feature, Antarctica ; Vaisala Integrated Met Station near Blood Falls, Taylor Glacier", "datasets": [{"dataset_uid": "601167", "doi": "10.15784/601167", "keywords": "Antarctica; Basal Crevassing; Glacier Hydrology; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Photo; Photo/video; Photo/Video; Snow/ice; Snow/Ice; Taylor Glacier; Timelaps Images", "people": "Pettit, Erin", "repository": "USAP-DC", "science_program": null, "title": "Time Lapse imagery of the Blood Falls feature, Antarctica ", "url": "https://www.usap-dc.org/view/dataset/601167"}, {"dataset_uid": "601168", "doi": "10.15784/601168", "keywords": "Antarctica; Atmosphere; Basal Crevassing; Glacier Hydrology; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Meteorology; Taylor Glacier; Temperature; Weather Station Data; Wind Speed", "people": "Pettit, Erin", "repository": "USAP-DC", "science_program": null, "title": "Vaisala Integrated Met Station near Blood Falls, Taylor Glacier", "url": "https://www.usap-dc.org/view/dataset/601168"}, {"dataset_uid": "200028", "doi": "10.7283/FCEN-8050", "keywords": null, "people": null, "repository": "UNAVCO", "science_program": null, "title": "Antarctica Support 2014/2015 - C-528 Blood Falls GPS/GNSS Observations Dataset", "url": "https://www.unavco.org/data/doi/10.7283/fcen-8050"}, {"dataset_uid": "200029", "doi": "10.7914/SN/YW_2013", "keywords": null, "people": null, "repository": "IRIS", "science_program": null, "title": "Blood Falls, McMurdo Dry Va. International Federation of Digital Seismograph Networks. Dataset/Seismic Network", "url": "http://www.fdsn.org/networks/detail/YW_2013/"}, {"dataset_uid": "601169", "doi": "10.15784/601169", "keywords": "Antarctica; Basal Crevassing; Glacier Hydrology; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Infrared Imagery; Photo/video; Photo/Video; Taylor Glacier; Thermal Camera; Timelaps Images", "people": "Pettit, Erin", "repository": "USAP-DC", "science_program": null, "title": "FLIR thermal imaging data near Blood Falls, Taylor Glacier", "url": "https://www.usap-dc.org/view/dataset/601169"}, {"dataset_uid": "601179", "doi": "10.15784/601179", "keywords": "Antarctica; Chemistry:ice; Chemistry:Ice; Geochemistry; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Subglacial Brine", "people": "Gardner, Christopher B.; Lyons, W. Berry", "repository": "USAP-DC", "science_program": null, "title": "The Geochemistry of englacial brine from Taylor Glacier, Antarctica", "url": "https://www.usap-dc.org/view/dataset/601179"}, {"dataset_uid": "601139", "doi": "10.15784/601139", "keywords": "Antarctica; Borehole; Borehole Logging; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Ice Temperature; Snow/ice; Snow/Ice; Temperature; Temperature Profiles", "people": "Tulaczyk, Slawek", "repository": "USAP-DC", "science_program": null, "title": "Ice Temperature in Shallow Boreholes Near Blood Falls at the Terminus of Taylor Glacier, McMurdo Dry Valleys, Antarctica", "url": "https://www.usap-dc.org/view/dataset/601139"}, {"dataset_uid": "601164", "doi": "10.15784/601164", "keywords": "Antarctica; Basal Crevassing; Glacier Hydrology; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Snow/ice; Snow/Ice", "people": "Pettit, Erin", "repository": "USAP-DC", "science_program": null, "title": "Ablation Stake Data from of Taylor Glacier near Blood Falls", "url": "https://www.usap-dc.org/view/dataset/601164"}, {"dataset_uid": "601165", "doi": "10.15784/601165", "keywords": "Antarctica; Basal Crevassing; Glacier Hydrology; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; GPR; Radar; Snow/ice; Snow/Ice; Taylor Glacier", "people": "Pettit, Erin", "repository": "USAP-DC", "science_program": null, "title": "Ground Penetrating Radar Data near Blood Falls, Taylor Glacier", "url": "https://www.usap-dc.org/view/dataset/601165"}, {"dataset_uid": "200074", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "NCBI short read archive -Metagenomic survey of Antarctic Groundwater", "url": "https://www.ncbi.nlm.nih.gov/sra/?term=SRR6667787"}, {"dataset_uid": "601166", "doi": "10.15784/601166", "keywords": "Antarctica; Basal Crevassing; Glacier Hydrology; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; GPR; Radar; Snow/ice; Snow/Ice; Taylor Glacier", "people": "Pettit, Erin", "repository": "USAP-DC", "science_program": null, "title": "Terrestrial Radar Interferometry near Blood Falls, Taylor Glacier", "url": "https://www.usap-dc.org/view/dataset/601166"}], "date_created": "Wed, 28 Nov 2018 00:00:00 GMT", "description": "Recent discoveries of widespread liquid water and microbial ecosystems below the Antarctic ice sheets have generated considerable interest in studying Antarctic subglacial environments. Understanding subglacial hydrology, the persistence of life in extended isolation and the evolution and stability of subglacial habitats requires an integrated, interdisciplinary approach. The collaborative project, Minimally Invasive Direct Glacial Exploration (MIDGE) of the Biogeochemistry, Hydrology and Glaciology of Blood Falls, McMurdo Dry Valleys will integrate geophysical measurements, molecular microbial ecology and geochemical analyses to explore a unique Antarctic subglacial system known as Blood Falls. Blood Falls is a hypersaline, subglacial brine that supports an active microbial community. The subglacial brine is released from a crevasse at the surface of the Taylor Glacier providing an accessible portal into an Antarctic subglacial ecosystem. Recent geochemical and molecular analyses support a marine source for the salts and microorganisms in Blood Falls. The last time marine waters inundated this part of the McMurdo Dry Valleys was during the Late Tertiary, which suggests the brine is ancient. Still, no direct samples have been collected from the subglacial source to Blood Falls and little is known about the origin of this brine or the amount of time it has been sealed below Taylor Glacier. Radar profiles collected near Blood Falls delineate a possible fault in the subglacial substrate that may help explain the localized and episodic nature of brine release. However it remains unclear what triggers the episodic release of brine exclusively at the Blood Falls crevasse or the extent to which the brine is altered as it makes its way to the surface. \u003cbr/\u003e\u003cbr/\u003eThe MIDGE project aims to determine the mechanism of brine release at Blood Falls, evaluate changes in the geochemistry and the microbial community within the englacial conduit and assess if Blood Falls waters have a distinct impact on the thermal and stress state of Taylor Glacier, one of the most studied polar glaciers in Antarctica. The geophysical study of the glaciological structure and mechanism of brine release will use GPR, GPS, and a small passive seismic network. Together with international collaborators, the \u0027Ice Mole\u0027 team from FH Aachen University of Applied Sciences, Germany (funded by the German Aerospace Center, DLR), MIDGE will develop and deploy innovative, minimally invasive technologies for clean access and brine sample retrieval from deep within the Blood Falls drainage system. These technologies will allow for the collection of samples of the brine away from the surface (up to tens of meters) for geochemical analyses and microbial structure-function experiments. There is concern over the contamination of pristine subglacial environments from chemical and biological materials inherent in the drilling process; and MIDGE will provide data on the efficacy of thermoelectric probes for clean access and retrieval of representative subglacial samples. Antarctic subglacial environments provide an excellent opportunity for researching survivability and adaptability of microbial life and are potential terrestrial analogues for life habitats on icy planetary bodies. The MIDGE project offers a portable, versatile, clean alternative to hot water and mechanical drilling and will enable the exploration of subglacial hydrology and ecosystem function while making significant progress towards developing technologies for minimally invasive and clean sampling of icy systems.", "east": 162.6, "geometry": "POINT(162.2 -77.735)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "NOT APPLICABLE; BACTERIA/ARCHAEA; USAP-DC", "locations": null, "north": -77.7, "nsf_funding_programs": "Antarctic Instrumentation and Support; Antarctic Integrated System Science; Antarctic Integrated System Science; Antarctic Integrated System Science; Antarctic Integrated System Science", "paleo_time": null, "persons": "Tulaczyk, Slawek; Pettit, Erin; Lyons, W. Berry; Mikucki, Jill", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "USAP-DC", "repositories": "IRIS; NCBI GenBank; UNAVCO; USAP-DC", "science_programs": null, "south": -77.77, "title": "Collaborative Research: MIDGE: Minimally Invasive Direct Glacial Exploration of Biogeochemistry, Hydrology and Glaciology of Blood Falls, McMurdo Dry Valleys", "uid": "p0000002", "west": 161.8}, {"awards": "1543245 Rynearson, Tatiana", "bounds_geometry": null, "dataset_titles": "Expedition Data; Expedition data of NBP1701; NCBI Popset of 43 Southern Ocean diatom isolates, including accessions ON678208.1 - ON678250.1; Specific growth rate measurements for 43 Southern Ocean diatoms", "datasets": [{"dataset_uid": "001369", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/NBP1701"}, {"dataset_uid": "200328", "doi": "", "keywords": null, "people": null, "repository": "NCBI", "science_program": null, "title": "NCBI Popset of 43 Southern Ocean diatom isolates, including accessions ON678208.1 - ON678250.1", "url": "https://www.ncbi.nlm.nih.gov/popset/?term=2248543458"}, {"dataset_uid": "601586", "doi": "10.15784/601586", "keywords": "Antarctica; Biota; NBP1701; Phytoplankton; R/v Nathaniel B. Palmer; Specific Growth Rate; Thermal Optimum Temperature", "people": "Bishop, Ian", "repository": "USAP-DC", "science_program": null, "title": "Specific growth rate measurements for 43 Southern Ocean diatoms", "url": "https://www.usap-dc.org/view/dataset/601586"}, {"dataset_uid": "002661", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of NBP1701", "url": "https://www.rvdata.us/search/cruise/NBP1701"}], "date_created": "Fri, 29 Dec 2017 00:00:00 GMT", "description": "The research will examine how diatoms (an important group of plankton in the Southern Ocean) adapt to environmental change. Diatoms will be sampled from different regions of the Southern Ocean, including the Drake Passage, the Pacific Sector of the Southern Ocean and the Ross Sea and examined to determine the range of genetic variation among diatoms in these regions. Experiments on a range of diatoms will be conducted in home laboratories and will be aimed at measuring shifts in physiological capacities over many generations in response to directional changes in the environment (temperature and pH). The information on the genetic diversity of field populations combined with information on potential rates of adaptability and genome changes will provide insight into ways in which polar marine diatoms populations may respond to environmental changes that may occur in surface oceans in the future or may have occurred during past climate conditions. Such information allows better modeling of biogeochemical cycles in the ocean as well as improves our abilities to interpret records of past ocean conditions. The project will support a doctoral student and a postdoctoral researcher as well as several undergraduate students. These scientists will learn the fundamentals of experimental evolution, a skill set that is being sought in the fields of biology and oceanography. The project also includes a collaboration with the Metcalf Institute for Marine and Environmental Reporting that will design and facilitate a session focused on current research related to evolution and climate change to be held at the annual conference of the National Association of Science Writers (NASW). \u003cbr/\u003e\u003cbr/\u003eBoth physiological and genetic variation are key parameters for understanding evolutionary processes in phytoplankton but they are essentially unknown for Southern Ocean diatoms. The extent to which these two factors determine plasticity and adaptability in field populations and the interaction between them will influence how and whether cold-adapted diatoms can respond to changing environments. This project includes a combination of field work to identify genetic diversity within diatoms using molecular approaches and experiments in the lab to assess the range of physiological variation in contemporary populations of diatoms and evolution experiments in the lab to assess how the combination of genetic diversity and physiological variation influence the evolutionary potential of diatoms under a changing environment. This research will uncover general relationships between physiological variation, genetic diversity, and evolutionary potential that may apply across microbial taxa and geographical regions, substantially improving efforts to predict shifts in marine ecosystems. Results from this study can be integrated into developing models that incorporate evolution to predict ecosystem changes under future climate change scenarios.", "east": null, "geometry": null, "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; IN SITU/LABORATORY INSTRUMENTS \u003e CHEMICAL METERS/ANALYZERS \u003e FLUOROMETERS; IN SITU/LABORATORY INSTRUMENTS \u003e MAGNETIC/MOTION SENSORS \u003e GRAVIMETERS \u003e GRAVIMETERS; IN SITU/LABORATORY INSTRUMENTS \u003e RECORDERS/LOGGERS \u003e AWS; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; EARTH REMOTE SENSING INSTRUMENTS \u003e ACTIVE REMOTE SENSING \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ECHO SOUNDERS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e MBES; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e THERMOSALINOGRAPHS", "is_usap_dc": true, "keywords": "USAP-DC; NBP1701; R/V NBP; AMD; USA/NSF; Amd/Us; DIATOMS", "locations": null, "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Rynearson, Tatiana; Bishop, Ian", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repo": "R2R", "repositories": "NCBI; R2R; USAP-DC", "science_programs": null, "south": null, "title": "NSFGEO-NERC: Evolutionary Response of Southern Ocean Diatoms to Environmental Change", "uid": "p0000850", "west": null}, {"awards": "1245703 Manahan, Donal", "bounds_geometry": "POLYGON((-68.0574 -52.7267,-67.39775 -52.7267,-66.7381 -52.7267,-66.07845 -52.7267,-65.4188 -52.7267,-64.75915 -52.7267,-64.0995 -52.7267,-63.43985 -52.7267,-62.7802 -52.7267,-62.12055 -52.7267,-61.4609 -52.7267,-61.4609 -53.95849,-61.4609 -55.19028,-61.4609 -56.42207,-61.4609 -57.65386,-61.4609 -58.88565,-61.4609 -60.11744,-61.4609 -61.34923,-61.4609 -62.58102,-61.4609 -63.81281,-61.4609 -65.0446,-62.12055 -65.0446,-62.7802 -65.0446,-63.43985 -65.0446,-64.0995 -65.0446,-64.75915 -65.0446,-65.4188 -65.0446,-66.07845 -65.0446,-66.7381 -65.0446,-67.39775 -65.0446,-68.0574 -65.0446,-68.0574 -63.81281,-68.0574 -62.58102,-68.0574 -61.34923,-68.0574 -60.11744,-68.0574 -58.88565,-68.0574 -57.65386,-68.0574 -56.42207,-68.0574 -55.19028,-68.0574 -53.95849,-68.0574 -52.7267))", "dataset_titles": "Expedition Data", "datasets": [{"dataset_uid": "001372", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG1606"}], "date_created": "Fri, 29 Dec 2017 00:00:00 GMT", "description": "This project will support two training courses that will introduce early-career scientists from a wide range of disciplinary backgrounds to key issues in polar science, and especially to provide the opportunity to gain hands-on experience in Antarctic field activities. Antarctica is an ideal location to study a wide variety of questions in biology. However, few students and early-career scientists have the opportunity to work on-site in Antarctica unless they are directly associated with a senior scientist who has a funded Antarctic project. The project will further the NSF goal of training new generations of scientists by providing hands-on training in Antarctica during one course at Palmer Station in 2016 and another at McMurdo Station in 2018. This represents a continuation of nine previous courses at McMurdo Station which have a proven record of introducing participants to Antarctic science under realistic field conditions, providing opportunities to understand and appreciate the complexities and logistical challenges of undertaking science in Antarctica, enhancing the professional careers of the participants, and increasing international collaborations for early-career scientists.\u003cbr/\u003e\u003cbr/\u003eThe proposed training courses will be open to Ph.D. students and post-doctoral scientists who have interests in the study of Antarctic marine organisms to help prepare them for success in developing their own independent research programs in polar regions. Long-standing and recent questions in evolution and ecology of Antarctic organisms will be examined with 1) field collections, 2) physiological experiments on whole organisms, 3) studies of isolated cells and tissues, 4) experiments on macromolecular processes (e.g., enzymes), and 5) molecular biological analyses.", "east": -61.4609, "geometry": "POINT(-64.75915 -58.88565)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; IN SITU/LABORATORY INSTRUMENTS \u003e CHEMICAL METERS/ANALYZERS \u003e FLUOROMETERS; IN SITU/LABORATORY INSTRUMENTS \u003e RECORDERS/LOGGERS \u003e AWS; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e XBT; EARTH REMOTE SENSING INSTRUMENTS \u003e ACTIVE REMOTE SENSING \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ECHO SOUNDERS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e THERMOSALINOGRAPHS", "is_usap_dc": false, "keywords": "R/V LMG; LMG1606", "locations": null, "north": -52.7267, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Manahan, Donal", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "R2R", "repositories": "R2R", "science_programs": null, "south": -65.0446, "title": "Collaborative Research: Biological Adaptations to Environmental Change in Antarctica - An Advanced Training Program for Early Career Scientists", "uid": "p0000392", "west": -68.0574}, {"awards": "1443474 Jenkins, Bethany", "bounds_geometry": null, "dataset_titles": "Expedition data of NBP1608", "datasets": [{"dataset_uid": "002664", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of NBP1608", "url": "https://www.rvdata.us/search/cruise/NBP1608"}], "date_created": "Fri, 29 Dec 2017 00:00:00 GMT", "description": "This project focuses on an important group of photosynthetic algae in the Southern Ocean (SO), diatoms, and the roles associated bacterial communities play in modulating their growth. Diatom growth fuels the SO food web and balances atmospheric carbon dioxide by sequestering the carbon used for growth to the deep ocean on long time scales as cells sink below the surface. The diatom growth is limited by the available iron in the seawater, most of which is not freely available to the diatoms but instead is tightly bound to other compounds. The nature of these compounds and how phytoplankton acquire iron from them is critical to understanding productivity in this region and globally. The investigators will conduct experiments to characterize the relationship between diatoms, their associated bacteria, and iron in open ocean and inshore waters. Experiments will involve supplying nutrients at varying nutrient ratios to natural phytoplankton assemblages to determine how diatoms and their associated bacteria respond to different conditions. This will provide valuable data that can be used by climate and food web modelers and it will help us better understand the relationship between iron, a key nutrient in the ocean, and the organisms at the base of the food web that use iron for photosynthetic growth and carbon uptake. The project will also further the NSF goals of training new generations of scientists and of making scientific discoveries available to the general public. The project supports early career senior investigators and the training of graduate and undergraduate students as well as outreach activities with middle school Girl Scouts in Rhode Island, inner city middle and high school age girls in Virginia, and middle school girls in Florida.\u003cbr/\u003e\u003cbr/\u003eThe project combines trace metal biogeochemistry, phytoplankton cultivation, and molecular biology to address questions regarding the production of iron-binding compounds and the role of diatom-bacterial interactions in this iron-limited region. Iron is an essential micronutrient for marine phytoplankton. Phytoplankton growth in the SO is limited by a lack of sufficient iron, with important consequences for carbon cycling and climate in this high latitude regime. Some of the major outstanding questions in iron biogeochemistry relate to the organic compounds that bind \u003e99.9% of dissolved iron in surface oceans. The investigators\u0027 prior research in this region suggests that production of strong iron-binding compounds in the SO is linked to diatom blooms in waters with high nitrate to iron ratios. The sources of these compounds are unknown but the investigators hypothesize that they may be from bacteria, which are known to produce such compounds for their own use. The project will test three hypotheses concerning the production of these iron-binding compounds, limitations on the biological availability of iron even if present in high concentrations, and the roles of diatom-associated bacteria in these processes. Results from this project will provide fundamental information about the biogeochemical trigger, and biological sources and function, of natural strong iron-binding compound production in the SO, where iron plays a critical role in phytoplankton productivity, carbon cycling, and climate regulation.", "east": null, "geometry": null, "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; IN SITU/LABORATORY INSTRUMENTS \u003e CHEMICAL METERS/ANALYZERS \u003e FLUOROMETERS; IN SITU/LABORATORY INSTRUMENTS \u003e MAGNETIC/MOTION SENSORS \u003e GRAVIMETERS \u003e GRAVIMETERS; IN SITU/LABORATORY INSTRUMENTS \u003e RECORDERS/LOGGERS \u003e AWS; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; EARTH REMOTE SENSING INSTRUMENTS \u003e ACTIVE REMOTE SENSING \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ECHO SOUNDERS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e MBES; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e THERMOSALINOGRAPHS", "is_usap_dc": false, "keywords": "R/V NBP; NBP1608", "locations": null, "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Jenkins, Bethany", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repo": "R2R", "repositories": "R2R", "science_programs": null, "south": null, "title": "Collaborative Research: Investigating Iron-inding Ligands in Southern Ocean Diatom Communities: The Role of Diatom-Bacteria Associations", "uid": "p0000852", "west": null}, {"awards": "1341701 Bilyk, Kevin", "bounds_geometry": null, "dataset_titles": "Antarctic Ice fish; submission ID #SRP113562", "datasets": [{"dataset_uid": "000206", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Antarctic Ice fish; submission ID #SRP113562", "url": "https://www.ncbi.nlm.nih.gov/genbank/"}], "date_created": "Mon, 31 Jul 2017 00:00:00 GMT", "description": "This work will broaden our knowledge and insights into genetic trait loss or change accompanying species evolution in general as well as within the uniquely isolated and frigid Southern Ocean. The system of oxygen-carrying and related proteins being studied is very important to human health and the two proteins being specifically studied in this work (haptoglobin and hemopexin) have crucial roles in preventing excess iron loading in the kidneys. As such, the project has the potential to contribute novel insights that could be valuable to medical science. The project will also further the NSF goals of training new generations of scientists and of making scientific discoveries available to the general public. The lead principal investigator on the project is an early career scientist whose career development will be enhanced by this project. It will also support the training of several undergraduate students in molecular biology, protein biochemistry, and appreciation of the unique Antarctic fish fauna and environment. The project will contribute to a content-rich web site that will bring to the public the history of biological discoveries and sciences on fishes of the Southern Ocean and through this project the investigators will contribute to an annual polar event at a children\u0027s science museum. \u003cbr/\u003e\u003cbr/\u003eThe Antarctic icefishes have thrived despite the striking evolutionary loss of the normally indispensable respiratory protein hemoglobin in all species and myoglobin in some. Studies over the past decades have predominately focused on the mechanisms behind hemoprotein losses and the resulting compensatory adaptations in these fish, while evolutionary impact of such losses on the supporting protein genes and functions has remained unaddressed. This project investigates the evolutionary fate of two important partner proteins, the hemoglobin scavenger haptoglobin and the heme scavenger hemopexin (heme groups are the iron-containing functional group of proteins such as hemoglobin and myoglobin). With the permanent hemoglobin-null state in Antarctic icefishes, and particularly in dual hemoglobin- and myoglobin-null species, the preservation of a functional haptoglobin would seem unessential and the role of hemopexin likely diminished. This project seeks to resolve whether co-evolutionary loss or reduction of these supporting proteins occurred with the extinction of the hemoglobin trait in the icefishes, and the molecular mechanisms underlying such changes. The investigators envisage the cold and oxygen rich marine environment as the start of a cascade of relaxation of selection pressures. Initially this would have obviated the need for maintaining functional oxygen carrying proteins, ultimately leading to their permanent loss. These events in turn would have relaxed the maintenance of the network of supporting systems, leading to additional trait loss or change.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Bilyk, Kevin", "platforms": "Not provided", "repo": "NCBI GenBank", "repositories": "NCBI GenBank", "science_programs": null, "south": null, "title": "Evolutionary Fates of Hemoglobin and Heme Scavengers in White-blooded Antarctic Icefishes", "uid": "p0000396", "west": null}, {"awards": "1443554 Buys, Emmanuel", "bounds_geometry": "POLYGON((166.163 -76.665,166.2635 -76.665,166.364 -76.665,166.4645 -76.665,166.565 -76.665,166.6655 -76.665,166.766 -76.665,166.8665 -76.665,166.967 -76.665,167.0675 -76.665,167.168 -76.665,167.168 -76.782,167.168 -76.899,167.168 -77.016,167.168 -77.133,167.168 -77.25,167.168 -77.367,167.168 -77.484,167.168 -77.601,167.168 -77.718,167.168 -77.835,167.0675 -77.835,166.967 -77.835,166.8665 -77.835,166.766 -77.835,166.6655 -77.835,166.565 -77.835,166.4645 -77.835,166.364 -77.835,166.2635 -77.835,166.163 -77.835,166.163 -77.718,166.163 -77.601,166.163 -77.484,166.163 -77.367,166.163 -77.25,166.163 -77.133,166.163 -77.016,166.163 -76.899,166.163 -76.782,166.163 -76.665))", "dataset_titles": "Biosamples and observations from Weddell Seal colonies in McMurdo Sound during the 2015-2016 Antarctic field season", "datasets": [{"dataset_uid": "601028", "doi": "10.15784/601028", "keywords": "Antarctica; Biota; Mcmurdo Sound; Ross Sea; Sample/collection Description; Sample/Collection Description; Seals", "people": "Hindle, Allyson; Buys, Emmanuel", "repository": "USAP-DC", "science_program": null, "title": "Biosamples and observations from Weddell Seal colonies in McMurdo Sound during the 2015-2016 Antarctic field season", "url": "https://www.usap-dc.org/view/dataset/601028"}], "date_created": "Fri, 26 May 2017 00:00:00 GMT", "description": "The Weddell seal is a champion diving mammal. The physiology that permits these animals to sustain extended breath-hold periods and survive the extreme pressure of diving deep allows them to thrive in icy Antarctic waters. Key elements of their physiological specializations to breath-hold diving are their ability for remarkable adjustment of their heart and blood vessel system, coordinating blood pressure and flow to specific body regions based on their metabolic requirements, and their ability to sustain periods without oxygen. Identifying the details of these strategies has tremendous potential to better inform human medicine, helping us to develop novel therapies for cardiovascular trauma (e.g. stroke, heart attack) and diseases associated with blunted oxygen delivery to tissues (e.g. pneumonia, sepsis, or cancer). The goal of this project is to document specific genes that control these cardiovascular adjustments in seals, and to compare their abundance and activity with humans. Specifically, the investigators will study a signaling pathway that coordinates local blood flow. They will also use tissue samples to generate cultured cells from Weddell seals that can be used to study the molecular effects of low oxygen conditions in the laboratory. The project will further the NSF goals of training new generations of scientists and of making scientific discoveries available to the general public. The project will train a pre-veterinary student researcher will conduct public outreach via a center for community health improvement, a multicultural affairs office, and a public aquarium. The goal of this study is to unravel the molecular mechanisms underlying the dive response. A hallmark of the dive response is tissue-specific vascular system regulation, likely resulting from variation in both nerve inputs and in production of local signaling molecules produced by blood vessel cells. The investigators will use emerging genomic information to begin to unravel the genetics underlying redistribution of the circulation during diving. They will also directly test the hypothesis that modifications in the signaling system prevent local blood vessel changes under low oxygen conditions, thereby allowing the centrally mediated diving reflex to override local physiological responses and to control the constriction of blood vessel walls in Weddell seals. They will perform RNA-sequencing of Weddell seal tissues and use the resulting sequence, along with information from other mammals such as dog, to obtain a full annotation (identifying all genes based on named features of reference genomes) of the existing genome assembly for the Weddell seal, facilitating comparative and species-specific genomic research. They will also generate a Weddell seal pluripotent stem cell line which should be a valuable research tool for cell biologists, molecular biologists and physiologists that will allow them to further test their hypotheses. It is expected that the proposed studies will advance our knowledge of the biochemical and physiological adaptations that allow the Weddell seal to thrive in the Antarctic environment.", "east": 167.168, "geometry": "POINT(166.6655 -77.25)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -76.665, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Buys, Emmanuel; Costa, Daniel; Zapol, Warren; Hindle, Allyson", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -77.835, "title": "Unraveling the Genomic and Molecular Basis of the Dive Response: Nitric Oxide Signaling and Vasoregulation in the Weddell Seal", "uid": "p0000072", "west": 166.163}, {"awards": "0947821 Ashworth, Allan", "bounds_geometry": "POLYGON((-180 -85.095235,-145.3719418 -85.095235,-110.7438836 -85.095235,-76.1158254 -85.095235,-41.4877672 -85.095235,-6.859709 -85.095235,27.7683492 -85.095235,62.3964074 -85.095235,97.0244656 -85.095235,131.6525238 -85.095235,166.280582 -85.095235,166.280582 -85.0996451,166.280582 -85.1040552,166.280582 -85.1084653,166.280582 -85.1128754,166.280582 -85.1172855,166.280582 -85.1216956,166.280582 -85.1261057,166.280582 -85.1305158,166.280582 -85.1349259,166.280582 -85.139336,131.6525238 -85.139336,97.0244656 -85.139336,62.3964074 -85.139336,27.7683492 -85.139336,-6.859709 -85.139336,-41.4877672 -85.139336,-76.1158254 -85.139336,-110.7438836 -85.139336,-145.3719418 -85.139336,180 -85.139336,178.6280582 -85.139336,177.2561164 -85.139336,175.8841746 -85.139336,174.5122328 -85.139336,173.140291 -85.139336,171.7683492 -85.139336,170.3964074 -85.139336,169.0244656 -85.139336,167.6525238 -85.139336,166.280582 -85.139336,166.280582 -85.1349259,166.280582 -85.1305158,166.280582 -85.1261057,166.280582 -85.1216956,166.280582 -85.1172855,166.280582 -85.1128754,166.280582 -85.1084653,166.280582 -85.1040552,166.280582 -85.0996451,166.280582 -85.095235,167.6525238 -85.095235,169.0244656 -85.095235,170.3964074 -85.095235,171.7683492 -85.095235,173.140291 -85.095235,174.5122328 -85.095235,175.8841746 -85.095235,177.2561164 -85.095235,178.6280582 -85.095235,-180 -85.095235))", "dataset_titles": "Neogene Paleoecology of the Beardmore Glacier Region", "datasets": [{"dataset_uid": "600387", "doi": "10.15784/600387", "keywords": "Antarctica; Beardmore Glacier; Biota; Fossil; GPS; Oliver Bluffs; Paleoclimate; Sample/collection Description; Sample/Collection Description; Seeds; Solid Earth; Transantarctic Mountains", "people": "Ashworth, Allan", "repository": "USAP-DC", "science_program": null, "title": "Neogene Paleoecology of the Beardmore Glacier Region", "url": "https://www.usap-dc.org/view/dataset/600387"}], "date_created": "Thu, 12 Jan 2017 00:00:00 GMT", "description": "Intellectual Merit: \u003cbr/\u003eThe primary goal of this project is to sample two beds in the Meyer Desert Formation, which are known to be especially fossiliferous containing plants, insects, other arthropods, freshwater mollusks, and fish. There is a possibility that the teeth and bones of a small marsupial could also be found. Previous studies have demonstrated that these horizons contain unique fossil assemblages that provide information used to reconstruct paleoenvironments and paleoclimate. The fossils represent organisms previously not found in Antarctica and consequently their study will lead to the development of new hypotheses concerning southern hemisphere biogeography. The new discoveries will also increase knowledge of paleoenvironments and paleoclimates as well as biogeographic relationships of the biota of the southern hemisphere. For some organisms, such as Nothofagus (Southern Beech) or the trechine groundbeetle, fossils would confirm that Antarctica was inhabited as part of Gondwana. For other fossils, such as the cyclorrhaphan fly or freshwater mollusks not expected to have inhabited Antarctica, the discoveries will require a reassessment of phylogenetic interpretations and a reinvestigation of the role of Antarctica in the evolutionary history of those organisms. The new fossil-based knowledge will require integration with interpretations from cladistics and molecular genetics to develop more comprehensive phylogenetic hypotheses for a range of organisms.\u003cbr/\u003e\u003cbr/\u003eBroader impacts: \u003cbr/\u003eThe discovery of fossils in Antarctica and implications for climate change has proven to be popular with the media. This attention will help disseminate the results of this study. Before the field season, the PI will work with local media and with area schools to set up field interviews and web casts from Antarctica. The project will also involve the training of a graduate student in the field and in the follow up studies of the fossils in the laboratory.", "east": 166.280582, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -85.095235, "nsf_funding_programs": "Antarctic Earth Sciences", "paleo_time": null, "persons": "Ashworth, Allan", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -85.139336, "title": "Neogene Paleoecology of the Beardmore Glacier Region", "uid": "p0000424", "west": 166.280582}, {"awards": "1142102 Takacs-Vesbach, Cristina; 1142096 Schwartz, Egbert", "bounds_geometry": "POLYGON((161 -77,161.3 -77,161.6 -77,161.9 -77,162.2 -77,162.5 -77,162.8 -77,163.1 -77,163.4 -77,163.7 -77,164 -77,164 -77.05,164 -77.1,164 -77.15,164 -77.2,164 -77.25,164 -77.3,164 -77.35,164 -77.4,164 -77.45,164 -77.5,163.7 -77.5,163.4 -77.5,163.1 -77.5,162.8 -77.5,162.5 -77.5,162.2 -77.5,161.9 -77.5,161.6 -77.5,161.3 -77.5,161 -77.5,161 -77.45,161 -77.4,161 -77.35,161 -77.3,161 -77.25,161 -77.2,161 -77.15,161 -77.1,161 -77.05,161 -77))", "dataset_titles": "GenBank. Accession # PRJNA232062,PRJNA228947,PRJNA228945; McMurdo Dry Valleys LTER Genetic/Genomic Data Resource; NCBI GenBank RNA sequences", "datasets": [{"dataset_uid": "000178", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "GenBank. Accession # PRJNA232062,PRJNA228947,PRJNA228945", "url": "http://www.ncbi.nlm.nih.gov/genbank/"}, {"dataset_uid": "000177", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "NCBI GenBank RNA sequences", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA356879"}, {"dataset_uid": "000180", "doi": "", "keywords": null, "people": null, "repository": "LTER", "science_program": null, "title": "McMurdo Dry Valleys LTER Genetic/Genomic Data Resource", "url": "http://www.mcmlter.org/genetic"}], "date_created": "Wed, 04 Jan 2017 00:00:00 GMT", "description": "The McMurdo Dry Valleys in Antarctica are among the coldest, driest habitats on the planet. Previous research has documented the presence of surprisingly diverse microbial communities in the soils of the Dry Valleys despite these extreme conditions. However, the degree to which these organisms are active is unknown; it is possible that much of this diversity reflects microbes that have blown into this environment that are subsequently preserved in these cold, dry conditions. This research will use modern molecular techniques to answer a fundamental question regarding these communities: which organisms are active and how do they live in such extreme conditions? The research will include manipulations to explore how changes in water, salt and carbon affect the microbial community, to address the role that these organisms play in nutrient cycling in this environment. The results of this work will provide a broader understanding of how life adapts to such extreme conditions as well as the role of dormancy in the life history of microorganisms. Results will be widely disseminated through publications as well as through presentations at national and international meetings; raw data will be made available through a high-profile web-based portal. The research will support two graduate students, two undergraduate research assistants and a postdoctoral fellow. The results will be incorporated into a webinar targeted to secondary and post-secondary educators and a complimentary hands-on class activity kit will be developed and made available to various teacher and outreach organizations.", "east": 164.0, "geometry": "POINT(162.5 -77.25)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -77.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Takacs-Vesbach, Cristina; Schwartz, Egbert; Van Horn, David", "platforms": "Not provided", "repo": "NCBI GenBank", "repositories": "LTER; NCBI GenBank", "science_programs": "LTER", "south": -77.5, "title": "Collaborative Research: An Integrated Ecological Investigation of McMurdo Dry Valley\u0027s Active Soil Microbial Communities", "uid": "p0000334", "west": 161.0}, {"awards": "1142018 Arrigo, Kevin", "bounds_geometry": "POLYGON((-75.8 -61.08,-74.457 -61.08,-73.114 -61.08,-71.771 -61.08,-70.428 -61.08,-69.085 -61.08,-67.742 -61.08,-66.399 -61.08,-65.056 -61.08,-63.713 -61.08,-62.37 -61.08,-62.37 -61.684,-62.37 -62.288,-62.37 -62.892,-62.37 -63.496,-62.37 -64.1,-62.37 -64.704,-62.37 -65.308,-62.37 -65.912,-62.37 -66.516,-62.37 -67.12,-63.713 -67.12,-65.056 -67.12,-66.399 -67.12,-67.742 -67.12,-69.085 -67.12,-70.428 -67.12,-71.771 -67.12,-73.114 -67.12,-74.457 -67.12,-75.8 -67.12,-75.8 -66.516,-75.8 -65.912,-75.8 -65.308,-75.8 -64.704,-75.8 -64.1,-75.8 -63.496,-75.8 -62.892,-75.8 -62.288,-75.8 -61.684,-75.8 -61.08))", "dataset_titles": "Adaptive Responses of Phaeocystis Populations in Antarctic Ecosystems; Expedition Data", "datasets": [{"dataset_uid": "001417", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/NBP1310"}, {"dataset_uid": "600161", "doi": "10.15784/600161", "keywords": "Antarctica; Antarctic Peninsula; Biota; Chlorophyll; CTD Data; NBP1310; NBP1409; Oceans; Physical Oceanography; Phytoplankton; Sea Surface; Southern Ocean", "people": "Arrigo, Kevin", "repository": "USAP-DC", "science_program": null, "title": "Adaptive Responses of Phaeocystis Populations in Antarctic Ecosystems", "url": "https://www.usap-dc.org/view/dataset/600161"}], "date_created": "Mon, 11 Jan 2016 00:00:00 GMT", "description": "Global climate change is having significant effects on areas of the Southern Ocean, and a better understanding of this ecosystem will permit predictions about the large-scale implications of these shifts. The haptophyte Phaeocystis antarctica is an important component of the phytoplankton communities in this region, but little is known about the factors controlling its distribution. Preliminary data suggest that P. antarctica posses unique adaptations that allow it to thrive in regions with dynamic light regimes. This research will extend these results to identify the physiological and genetic mechanisms that affect the growth and distribution of P. antarctica. This work will use field and laboratory-based studies and a suite of modern molecular techniques to better understand the biogeography and physiology of this key organism. Results will be widely disseminated through publications as well as through presentations at national and international meetings. In addition, raw data will be made available through open-access databases. This project will support the research and training of two graduate students and will foster an established international collaboration with Dutch scientists. Researchers on this project will participate in outreach programs targeting K12 teachers as well as high school students.", "east": -62.37, "geometry": "POINT(-69.085 -64.1)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; IN SITU/LABORATORY INSTRUMENTS \u003e CHEMICAL METERS/ANALYZERS \u003e FLUOROMETERS; IN SITU/LABORATORY INSTRUMENTS \u003e RECORDERS/LOGGERS \u003e AWS; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; EARTH REMOTE SENSING INSTRUMENTS \u003e ACTIVE REMOTE SENSING \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ECHO SOUNDERS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e MBES; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e THERMOSALINOGRAPHS", "is_usap_dc": true, "keywords": "R/V NBP; Not provided", "locations": null, "north": -61.08, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Arrigo, Kevin", "platforms": "Not provided; WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repo": "R2R", "repositories": "R2R; USAP-DC", "science_programs": null, "south": -67.12, "title": "Collaborative Research: Adaptive Responses of Phaeocystis Populations in Antarctic Ecosystems", "uid": "p0000446", "west": -75.8}, {"awards": "0944646 Bowser, Samuel", "bounds_geometry": "POLYGON((-160 -68,-159 -68,-158 -68,-157 -68,-156 -68,-155 -68,-154 -68,-153 -68,-152 -68,-151 -68,-150 -68,-150 -69,-150 -70,-150 -71,-150 -72,-150 -73,-150 -74,-150 -75,-150 -76,-150 -77,-150 -78,-151 -78,-152 -78,-153 -78,-154 -78,-155 -78,-156 -78,-157 -78,-158 -78,-159 -78,-160 -78,-160 -77,-160 -76,-160 -75,-160 -74,-160 -73,-160 -72,-160 -71,-160 -70,-160 -69,-160 -68))", "dataset_titles": "Evolution and Diversity of Antarctic Rhizarian Protists", "datasets": [{"dataset_uid": "600157", "doi": "10.15784/600157", "keywords": "Antarctica; Biota; Foraminifera; Oceans; Southern Ocean", "people": "Bowser, Samuel S.", "repository": "USAP-DC", "science_program": null, "title": "Evolution and Diversity of Antarctic Rhizarian Protists", "url": "https://www.usap-dc.org/view/dataset/600157"}], "date_created": "Wed, 23 Dec 2015 00:00:00 GMT", "description": "This research project will continue studies of a taxonomically definitive group of early evolving (single-chambered) Foraminifera at both multi-gene molecular and ultrastructural levels of analysis, in order to generate more robust and detailed phylogenies of these ecologically-important organisms. Studies will be extended to include members of the enigmatic genus Gromia, to better define their ecological significance and placement within the protistan supergroup Rhizara. Together, these structural and molecular data will be used to complete taxonomic descriptions of over 20 new rhizarian species. The research will develop rapid molecular screening methods for future ecological studies of rhizarian protists, and uncover the driving forces that led to their diversification. A range of educational activities will target local and national audiences, and include development of hands-on, standards-based, innovative science classroom activities. In addition, a short art/science documentary film depicting research on the biology and evolution of Antarctic rhizarian protists will form the basis of a workshop hosted by the Capital Region Center for Arts in Education.", "east": -150.0, "geometry": "POINT(-155 -73)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -68.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Bowser, Samuel", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -78.0, "title": "Evolution and Diversity of Antarctic Rhizarian Protists", "uid": "p0000413", "west": -160.0}, {"awards": "1141936 Foreman, Christine", "bounds_geometry": "POINT(112.085 -79.467)", "dataset_titles": "Molecular Level Characterization of Dissolved Organic Carbon and Microbial Diversity in the WAIS Divide Replicate Core", "datasets": [{"dataset_uid": "600133", "doi": "10.15784/600133", "keywords": "Antarctica; Biota; Genetic Sequences; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Ice Core Records; Paleoclimate; WAIS Divide; WAIS Divide Ice Core", "people": "Foreman, Christine", "repository": "USAP-DC", "science_program": "WAIS Divide Ice Core", "title": "Molecular Level Characterization of Dissolved Organic Carbon and Microbial Diversity in the WAIS Divide Replicate Core", "url": "https://www.usap-dc.org/view/dataset/600133"}], "date_created": "Thu, 05 Nov 2015 00:00:00 GMT", "description": "This award supports a detailed, molecular level characterization of dissolved organic carbon and microbes in Antarctic ice cores. Using the most modern biological (genomic), geochemical techniques, and advanced chemical instrumentation researchers will 1) optimize protocols for collecting, extracting and amplifying DNA from deep ice cores suitable for use in next generation pyrosequencing; 2) determine the microbial diversity within the ice core; and 3) obtain and analyze detailed molecular characterizations of the carbon in the ice by ultrahigh resolution Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS). With this pilot study investigators will be able to quantify the amount of material (microbial biomass and carbon) required to perform these characterizations, which is needed to inform future ice coring projects. The ultimate goal will be to develop protocols that maximize the yield, while minimizing the amount of ice required. The broader impacts include education and outreach at both the local and national levels. As a faculty mentor with the American Indian Research Opportunities and BRIDGES programs at Montana State University, Foreman will serve as a mentor to a Native American student in the lab during the summer months. Susan Kelly is an Education and Outreach Coordinator with a MS degree in Geology and over 10 years of experience in science outreach. She will coordinate efforts for comprehensive educational collaboration with the Hardin School District on the Crow Indian Reservation in South-central Montana.", "east": 112.085, "geometry": "POINT(112.085 -79.467)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e CHEMICAL METERS/ANALYZERS \u003e ADS; IN SITU/LABORATORY INSTRUMENTS \u003e CORERS \u003e CORING DEVICES; IN SITU/LABORATORY INSTRUMENTS \u003e SPECTROMETERS/RADIOMETERS \u003e MASS SPECTROMETERS", "is_usap_dc": true, "keywords": "Dissolved Organic Carbon; Microbes; Ice Core; Not provided; Pyrosequencing; Microbial Diversity; Molecular; WAIS Divide; LABORATORY; FIELD SURVEYS; Antarctic; FIELD INVESTIGATION; DNA", "locations": "Antarctic; WAIS Divide", "north": -79.467, "nsf_funding_programs": "Antarctic Glaciology", "paleo_time": null, "persons": "Foreman, Christine", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION; LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS; Not provided; OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": "WAIS Divide Ice Core", "south": -79.467, "title": "Molecular Level Characterization of Dissolved Organic Carbon and Microbial Diversity in the WAIS Divide Replicate Core", "uid": "p0000342", "west": 112.085}, {"awards": "0939628 Barletta, Robert", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": null, "datasets": null, "date_created": "Thu, 06 Aug 2015 00:00:00 GMT", "description": "Biogenic sulfur compounds, such as dimethyl sulfide (DMS), its precursors dimethyl sulfoniopropionate (DMSP) and dimethyl sulfoxide (DMSO), and its atmospheric oxidation product, methane sulfonic acid (MSA), are important components of the global sulfur cycle that significantly impact global climate. The roles of DMSP and DMSO within the organisms that produce them, as well as their intracellular concentrations, are poorly understood. DMSO has been speculated to play a role in intracellular osmoregulation, cryoprotection and scavenging of reactive oxygen species, but its intracellular concentration in plankton has only been inferred. Quantitative measurement of the concentration of biogenic sulfur compounds in vivo is necessary to more completely understand their biogeochemistry. The principal investigator has developed methods for the quantitative analysis of biogenic sulfur compounds using Raman spectroscopy, which have resulted in the detection of DMSO with a sensitivity of \u003c10 mM - far lower than the current estimates of its intracellular concentrations. The research will extend this technique to DMSP. The direct determination of the intracellular DMSP and DMSO, will allow the proposed roles of these compounds in phytoplankton to be investigated. Lastly, using field-collected cores, measurements will be made of the intracellular sulfur compounds as well as the concentration of molecular anions in the sea ice micro-environment. As an RUI project, successful completion of this work will have a substantial impact on undergraduate education in the Chemistry Department at the University of South Alabama, exposing undergraduates and, particularly, under-represented minorities in the sciences to cutting-edge research. It will provide financial support for their education and allow them to present research in journal articles and at technical meetings. Contacts with scientists in the field of Antarctic research at other institutions will give students the opportunity to interact with researchers in related fields, broadening their experience base.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Barletta, Robert", "platforms": "Not provided", "repositories": null, "science_programs": null, "south": -90.0, "title": "RUI: Analysis of Intracellular Biogenic Sulfur Using micro-Raman Spectroscopy", "uid": "p0000403", "west": -180.0}, {"awards": "1143619 Severinghaus, Jeffrey", "bounds_geometry": "POINT(-112.09 -79.47)", "dataset_titles": null, "datasets": null, "date_created": "Mon, 13 Jul 2015 00:00:00 GMT", "description": "This award supports a project to extend the study of gases in ice cores to those gases whose small molecular diameters cause them to escape rapidly from ice samples (the so-called \"fugitive gases\"). The work will employ helium, neon, argon, and oxygen measurements in the WAIS Divide ice core to better understand the mechanism of the gas close-off fractionation that occurs while air bubbles are incorporated into ice. The intellectual merit of the proposed work is that corrections for this fractionation using neon (which is constant in the atmosphere) may ultimately enable the first ice core-based atmospheric oxygen and helium records. Neon may also illuminate the mechanistic link between local insolation and oxygen used for astronomical dating of ice cores. Helium measure-ments in the deepest ~100 m of the core will also shed light on the stratigraphic integrity of the basal ice, and serve as a probe of solid earth-ice interaction at the base of the West Antarctic ice sheet. Past atmospheric oxygen records, currently unavailable prior to 1989 CE, would reveal changes in the size of the terrestrial biosphere carbon pool that accompany climate variations and place constraints on the biogeochemical feedback response to future warming. An atmospheric helium-3/helium-4 record would test the hypothesis that the solar wind (which is highly enriched in helium-3) condensed directly into Earth?s atmosphere during the collapse of the geomagnetic field that occurred 41,000 years ago, known as the Laschamp Event. Fugitive-gas samples will be taken on-site immediately after recovery of the ice core by the PI and one postdoctoral scholar, under the umbrella of an existing project to support replicate coring and borehole deepening. This work will add value to the scientific return from field work activity with little additional cost to logistical resources. The broader impacts of the work on atmospheric oxygen are that it may increase understanding of how terrestrial carbon pools and atmospheric greenhouse gas sources will respond in a feedback sense to the coming warming. Long-term atmospheric oxygen trends are also of interest for understanding biogeochemical regulatory mechanisms and the impact of atmospheric evolution on life. Helium records have value in understanding the budget of this non-renewable gas and its implications for space weather and solar activity. The project will train one graduate student and one postdoctoral scholar. The fascination of linking solid earth, cryosphere, atmosphere, and space weather will help to entrain and excite young scientists and efforts to understand the Earth as a whole interlinked system will provide fuel to outreach efforts at all ages.", "east": -112.09, "geometry": "POINT(-112.09 -79.47)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e SPECTROMETERS/RADIOMETERS \u003e MASS SPECTROMETERS", "is_usap_dc": false, "keywords": "WAIS Divide; Not provided; Tracers; FIELD INVESTIGATION; Past Biospheric Carbon Storage; LABORATORY; Fugitive Gases; Basal Processes; Neon; Helium; FIELD SURVEYS; Antarctica", "locations": "WAIS Divide; Antarctica", "north": -79.47, "nsf_funding_programs": "Antarctic Glaciology", "paleo_time": null, "persons": "Severinghaus, Jeffrey P.", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION; LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS; Not provided; OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repositories": null, "science_programs": null, "south": -79.47, "title": "Fugitive Gases (Helium, Neon, and Oxygen) in the WAIS Divide Ice Core as Tracers of Basal Processes and Past Biospheric Carbon Storage", "uid": "p0000441", "west": -112.09}, {"awards": "1044982 Bucklin, Ann", "bounds_geometry": "POLYGON((-69.3804 -52.760597,-67.79698 -52.760597,-66.21356 -52.760597,-64.63014 -52.760597,-63.04672 -52.760597,-61.4633 -52.760597,-59.87988 -52.760597,-58.29646 -52.760597,-56.71304 -52.760597,-55.12962 -52.760597,-53.5462 -52.760597,-53.5462 -53.9928073,-53.5462 -55.2250176,-53.5462 -56.4572279,-53.5462 -57.6894382,-53.5462 -58.9216485,-53.5462 -60.1538588,-53.5462 -61.3860691,-53.5462 -62.6182794,-53.5462 -63.8504897,-53.5462 -65.0827,-55.12962 -65.0827,-56.71304 -65.0827,-58.29646 -65.0827,-59.87988 -65.0827,-61.4633 -65.0827,-63.04672 -65.0827,-64.63014 -65.0827,-66.21356 -65.0827,-67.79698 -65.0827,-69.3804 -65.0827,-69.3804 -63.8504897,-69.3804 -62.6182794,-69.3804 -61.3860691,-69.3804 -60.1538588,-69.3804 -58.9216485,-69.3804 -57.6894382,-69.3804 -56.4572279,-69.3804 -55.2250176,-69.3804 -53.9928073,-69.3804 -52.760597))", "dataset_titles": "Expedition Data", "datasets": [{"dataset_uid": "001445", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG1110"}], "date_created": "Wed, 17 Jun 2015 00:00:00 GMT", "description": "The proposed research targets the molecular genetics of salps, an increasingly important member of the zooplankton in Antarctic waters who\u0027s dominance appears to be related to climate warming and sea ice loss. Specifically the research will examine genome-wide patterns of gene expression, target gene expression levels, and patterns of population genetic diversity and structure underlying the complex life history and population dynamics of S. thompsoni. The P.I.s hypothesize that (1) deep analysis of the Salpa thompsoni transcriptome will reveal significant associations among selected set of differentially expressed genes and critical life history stages and events (e.g., ontogenetic maturation, sexual reproduction, senescence) of the salp; and 2) the species will show variable levels of clonal diversity and significant genetic differentiation among populations in different regions of the Southern Ocean. Broader impacts include training of two graduate students; inclusion of undergraduates in research, and in a formal training workshop; development of a summer workshop for high school teachers in collaboration with Connecticut Sea Grant; and public outreach via postings on the Census of Marine Zooplankton homepage.", "east": -53.5462, "geometry": "POINT(-61.4633 -58.9216485)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; IN SITU/LABORATORY INSTRUMENTS \u003e CHEMICAL METERS/ANALYZERS \u003e FLUOROMETERS; IN SITU/LABORATORY INSTRUMENTS \u003e RECORDERS/LOGGERS \u003e AWS; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e XBT; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ADCP; EARTH REMOTE SENSING INSTRUMENTS \u003e ACTIVE REMOTE SENSING \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ECHO SOUNDERS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e THERMOSALINOGRAPHS", "is_usap_dc": false, "keywords": "PLANKTON; Antarctic Peninsula; R/V LMG", "locations": "Antarctic Peninsula", "north": -52.760597, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Bucklin, Ann", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "R2R", "repositories": "R2R", "science_programs": null, "south": -65.0827, "title": "Population ecology of Salpa thompsoni based on molecular indicators", "uid": "p0000508", "west": -69.3804}, {"awards": "0944556 Barrett, John", "bounds_geometry": "POLYGON((160.6015 -76.9089,161.7382 -76.9089,162.8749 -76.9089,164.0116 -76.9089,165.1483 -76.9089,166.285 -76.9089,167.4217 -76.9089,168.5584 -76.9089,169.6951 -76.9089,170.8318 -76.9089,171.9685 -76.9089,171.9685 -77.73527,171.9685 -78.56164,171.9685 -79.38801,171.9685 -80.21438,171.9685 -81.04075,171.9685 -81.86712,171.9685 -82.69349,171.9685 -83.51986,171.9685 -84.34623,171.9685 -85.1726,170.8318 -85.1726,169.6951 -85.1726,168.5584 -85.1726,167.4217 -85.1726,166.285 -85.1726,165.1483 -85.1726,164.0116 -85.1726,162.8749 -85.1726,161.7382 -85.1726,160.6015 -85.1726,160.6015 -84.34623,160.6015 -83.51986,160.6015 -82.69349,160.6015 -81.86712,160.6015 -81.04075,160.6015 -80.21438,160.6015 -79.38801,160.6015 -78.56164,160.6015 -77.73527,160.6015 -76.9089))", "dataset_titles": "Ecosphere (Supplement), Ecological Society of America.", "datasets": [{"dataset_uid": "002538", "doi": "", "keywords": null, "people": null, "repository": "Publication", "science_program": null, "title": "Ecosphere (Supplement), Ecological Society of America.", "url": "http://www.esapubs.org/archive/ecos/C004/014/suppl-1.php"}], "date_created": "Fri, 13 Feb 2015 00:00:00 GMT", "description": "Advances in molecular techniques have expanded our understanding of soil microbial communities, and raised important questions about regional and global patterns in microbial diversity. The proposed research will investigate the composition and activity of microbial communities across a range of geochemical and hydrologic soil conditions, and over local to regional scales in the Transantarctic Mountains, in order to assess controls over microbial biogeography. The research targets two areas in the Transantarctic mountains, the McMurdo Dry Valleys, and the Beardmore Glacier region further south, the latter representing an underexplored and inarguably more extreme soil environment. The research project will adopt an integrated approach, using molecular techniques and in situ assessment of biological activity in a quantitative biogeographical framework, with the goal of distinguishing fine versus broad scale controls over microbial community structure. The research is essential to determining the basic trophic status of extreme microbial food webs, and their sensitivity to climate change. The investigators will engage secondary and post-secondary educators through first person outreach as well as web-based communications and exercises. Two postdoctoral scientists will be trained in an interdisciplinary and international setting.", "east": 171.9685, "geometry": "POINT(166.285 -81.04075)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -76.9089, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Barrett, John", "platforms": "Not provided", "repo": "Publication", "repositories": "Publication", "science_programs": null, "south": -85.1726, "title": "Collaborative Research: Controls over the Spatial Distribution and Activity of Microbial Communities in Antarctic Soils", "uid": "p0000350", "west": 160.6015}, {"awards": "1019305 Grim, Jeffrey", "bounds_geometry": null, "dataset_titles": "Impact of Rising Oceanic Temperatures on the Embryonic Development of Antarctic Notothenioid Fishes", "datasets": [{"dataset_uid": "600119", "doi": "10.15784/600119", "keywords": "Biota; Fish Logs; LMG1203; LMG1204; LMG1205; Oceans; Pot; Southern Ocean; Trawl", "people": "Grim, Jeffrey", "repository": "USAP-DC", "science_program": null, "title": "Impact of Rising Oceanic Temperatures on the Embryonic Development of Antarctic Notothenioid Fishes", "url": "https://www.usap-dc.org/view/dataset/600119"}], "date_created": "Mon, 10 Feb 2014 00:00:00 GMT", "description": "Survival of Antarctic notothenioid fishes in the context of global climate change will depend upon the impact of rising oceanic temperatures on their embryonic development, yet little is known regarding the molecular mechanisms underlying this complex suite of processes. Many notothenioids are characterized by secondary pelagicism, which enables them to exploit food sources in the water column and is supported in part by skeletal pedomorphism. Here the PI proposes to examine the hypothesis that reactive oxygen species (ROS) regulate notothenioid skeletal pedomorphism. The PI proposes to examine the hypothesis that reactive oxygen species (ROS) regulate notothenioid skeletal pedomorphism. The research objectives are : 1) To quantify and localize ROS production and identify the point(s) of origin of ROS production in embryonic Antarctic fishes that differ in skeletal phenotypes 2) To determine whether the time course of embryogenesis and the extent of osteological development in embryonic Antarctic fishes can be altered by changing the oxidative status of the animal during embryogenesis 3) To evaluate whether transgenic alteration of oxidative status can induce skeletal pedomorphism in a fish model. Broader Impacts will include teaching undergraduate lectures, recruiting undergraduate students to help with lab analyses (and possibly field work), lectures and demonstrations to high school students, and allowing secondary educators access to personal photos and videos of research animals for curriculum development.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Grim, Jeffrey", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": null, "title": "PostDoctoral Research Fellowship", "uid": "p0000482", "west": null}, {"awards": "0944517 Detrich, H. William", "bounds_geometry": "POLYGON((-67.42 -61.2,-66.28 -61.2,-65.14 -61.2,-64 -61.2,-62.86 -61.2,-61.72 -61.2,-60.58 -61.2,-59.44 -61.2,-58.3 -61.2,-57.16 -61.2,-56.02 -61.2,-56.02 -61.71,-56.02 -62.22,-56.02 -62.73,-56.02 -63.24,-56.02 -63.75,-56.02 -64.26,-56.02 -64.77,-56.02 -65.28,-56.02 -65.79,-56.02 -66.3,-57.16 -66.3,-58.3 -66.3,-59.44 -66.3,-60.58 -66.3,-61.72 -66.3,-62.86 -66.3,-64 -66.3,-65.14 -66.3,-66.28 -66.3,-67.42 -66.3,-67.42 -65.79,-67.42 -65.28,-67.42 -64.77,-67.42 -64.26,-67.42 -63.75,-67.42 -63.24,-67.42 -62.73,-67.42 -62.22,-67.42 -61.71,-67.42 -61.2))", "dataset_titles": "Cruise LMG1203; Cruise LMG1205; Cruise LMG1206; GenBank KC594078, FJ914563.1, FJ914564.1, FJ914565.1, FJ896018.1, FJ896019.1, FJ896022.1, FJ896023.1, FJ896024.1, FJ896025.1, FJ896026.1", "datasets": [{"dataset_uid": "000159", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "GenBank KC594078, FJ914563.1, FJ914564.1, FJ914565.1, FJ896018.1, FJ896019.1, FJ896022.1, FJ896023.1, FJ896024.1, FJ896025.1, FJ896026.1", "url": "http://www.ncbi.nlm.nih.gov/genbank/"}, {"dataset_uid": "000160", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Cruise LMG1203", "url": "https://www.rvdata.us/search/cruise/LMG1203"}, {"dataset_uid": "000161", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Cruise LMG1205", "url": "https://www.rvdata.us/search/cruise/LMG1205"}, {"dataset_uid": "000162", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Cruise LMG1206", "url": "https://www.rvdata.us/search/cruise/LMG1206"}], "date_created": "Fri, 20 Dec 2013 00:00:00 GMT", "description": "Since the advent of Antarctic continental glaciation, the opening of the Drake Passage between South America and the Antarctic Peninsula, and the onset of cooling of the Southern Ocean ~40-25 million years ago, evolution of the Antarctic marine biota has been driven by the development of extreme cold temperatures. The biochemical and physiological challenges facing ectotherms living in the Southern Ocean include the reduction of reaction rates and metabolic fluxes and a pervasive weakening of macromolecular interactions. Yet, Southern Ocean ectotherms are now threatened by warming over periods measured in centuries or less. The proposed research seeks to understand the molecular mechanisms of cold adaptation in Antarctic marine fishes and to assess the physiological capacity of these organisms to resist or compensate for rapid oceanic warming. The P.I. will characterize two important and interacting protein systems, the tubulins that form microtubules and the chaperonin CCT (cytoplasmic chaperonin-containing TCP-1, a family of proteins that assists the folding of the tubulins). Higher-level, integrative responses to global temperature change will be analyzed by studying the thermal dependence of cleavage in Antarctic fish embryos (a microtubule-dependent process). The objectives are (1) to determine the contributions of five novel amino acid substitutions found in Antarctic fish beta-tubulins to microtubule assembly at cold temperature. (2) to compare the functional properties of CCT from testis tissues of Antarctic fishes and mammals. (3) to evaluate the effects of increased temperature on embryogenesis in Antarctic fishes. The research will introduce graduate and REU undergraduate students to state-of-the-art biochemical, cellular, and molecular-biological research relevant to ecological and environmental issues of the Antarctic marine ecosystem. The proposed work also will benefit society by developing a cold-functioning chaperonin protein folding system, of great value to the biopharmaceutical and biotechnological industries for use in folding insoluble proteins.", "east": -56.02, "geometry": "POINT(-61.72 -63.75)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -61.2, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Detrich, H. William", "platforms": "Not provided", "repo": "NCBI GenBank", "repositories": "NCBI GenBank; R2R", "science_programs": null, "south": -66.3, "title": "Microtubule Function, Protein Folding, and Embryogenesis in Antarctic Fishes: An Integrative Approach", "uid": "p0000664", "west": -67.42}, {"awards": "0739648 Cary, Stephen", "bounds_geometry": "POINT(163 -77.5)", "dataset_titles": "Biogeochemistry of Cyanobactrial Mats and Hyporheic Zone Microbes in McMurdo Dry Valley Glacial Meltwater Streams", "datasets": [{"dataset_uid": "600079", "doi": "10.15784/600079", "keywords": "Antarctica; Biota; Cell Counts; Dry Valleys; Microbiology", "people": "Cary, S. Craig", "repository": "USAP-DC", "science_program": null, "title": "Biogeochemistry of Cyanobactrial Mats and Hyporheic Zone Microbes in McMurdo Dry Valley Glacial Meltwater Streams", "url": "https://www.usap-dc.org/view/dataset/600079"}], "date_created": "Tue, 10 Dec 2013 00:00:00 GMT", "description": "The glacial streams of the McMurdo Dry Valleys have extensive cyanobacterial mats that are a probable source of fixed C and N to the Valleys. The research will examine the interplay between the microbial mats in the ephemeral glacial streams and the microbiota of the hyporheic soils (wetted soil zone) underlying and adjacent to those mats. It is hypothesized that the mats are important sources of organic carbon and fixed nitrogen for the soil communities of the hyporheic zone, and release dissolved organic carbon (DOC) and nitrogen (DON) that serves the entire Dry Valley ecosystem. Field efforts will entail both observational and experimental components. Direct comparisons will be made between the mats and microbial populations underlying naturally rehydrated and desiccated mat areas, and between mat areas in the melt streams of the Adams and Miers Glaciers in Miers Valley. Both physiological and phylogenetic indices of the soil microbiota will be examined. Observations will include estimates of rates of mat carbon and nitrogen fixation, soil respiration and leucine and thymidine uptake (as measures of protein \u0026 DNA synthesis, respectively) by soil bacteria, bacterial densities and their molecular ecology. Experimental manipulations will include experimental re-wetting of soils and observations of the time course of response of the microbial community. The research will integrate modern molecular genetic approaches (ARISA-DNA fingerprinting and ultra deep 16S rDNA microbial phylogenetic analysis) with geochemistry to study the diversity, ecology, and function of microbial communities that thrive in these extreme environments. The broader impacts of the project include research and educational opportunities for graduate students and a postdoctoral associate. The P.I.s will involve undergraduates as work-study students and in REU programs, and will participate in educational and outreach programs.", "east": 163.0, "geometry": "POINT(163 -77.5)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -77.5, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Cary, Stephen", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -77.5, "title": "Collaborative Research: Biogeochemistry of Cyanobactrial Mats and Hyporheic Zone Microbes in McMurdo Dry Valley Glacial Meltwater Streams", "uid": "p0000476", "west": 163.0}, {"awards": "0741301 O\u0027Brien, Kristin; 1142720 Crockett, Elizabeth", "bounds_geometry": "POLYGON((-64.45 -63.29,-64.249 -63.29,-64.048 -63.29,-63.847 -63.29,-63.646 -63.29,-63.445 -63.29,-63.244 -63.29,-63.043 -63.29,-62.842 -63.29,-62.641 -63.29,-62.44 -63.29,-62.44 -63.370999999999995,-62.44 -63.452,-62.44 -63.533,-62.44 -63.614,-62.44 -63.69499999999999,-62.44 -63.775999999999996,-62.44 -63.857,-62.44 -63.937999999999995,-62.44 -64.01899999999999,-62.44 -64.1,-62.641 -64.1,-62.842 -64.1,-63.043 -64.1,-63.244 -64.1,-63.445 -64.1,-63.646 -64.1,-63.847 -64.1,-64.048 -64.1,-64.249 -64.1,-64.45 -64.1,-64.45 -64.01899999999999,-64.45 -63.937999999999995,-64.45 -63.857,-64.45 -63.775999999999996,-64.45 -63.69499999999999,-64.45 -63.614,-64.45 -63.533,-64.45 -63.452,-64.45 -63.370999999999995,-64.45 -63.29))", "dataset_titles": "Linkages among Mitochondrial Form, Function and Thermal Tolerance of Antarctic Notothenioid Fishes", "datasets": [{"dataset_uid": "600084", "doi": "10.15784/600084", "keywords": "Biota; Oceans; Pot; Southern Ocean; Trawl", "people": "O\u0027Brien, Kristin", "repository": "USAP-DC", "science_program": null, "title": "Linkages among Mitochondrial Form, Function and Thermal Tolerance of Antarctic Notothenioid Fishes", "url": "https://www.usap-dc.org/view/dataset/600084"}], "date_created": "Sat, 30 Nov 2013 00:00:00 GMT", "description": "Antarctic notothenioid fishes have evolved in the Southern Ocean for 10-14 MY under an unusual set of circumstances. Their characteristics include the complete absence of the circulating oxygen-binding protein, hemoglobin (Hb) within the Channichthyid (Icefish) family of notothenioids. Moreover, some species within the 16 members of this family have also lost the ability to express the oxygen-binding and storage protein, myoglobin (Mb) in cardiac muscle. Our previous work has determined that the loss of Hb and/or Mb is correlated with significant increases in densities of mitochondria within oxidative tissues, and extensive remodeling of these vital organelles. To date, nothing is known about how modifications in mitochondrial architecture of icefishes affect organelle function, or more importantly, how they affect organismal-level physiology. Most critical for Antarctic fishes is that mitochondrial characteristics have been linked to how well ectotherms can withstand increases in temperature. \u003cbr/\u003e\u003cbr/\u003eThis collaborative research project will address the hypothesis that the unusual mitochondrial architecture of Antarctic Channichthyids has led to changes in function that impact their ability to withstand elevations in temperature. Specifically, the research will (1) determine if the unusual mitochondrial architecture of icefishes affects function and contributes to organismal thermal sensitivity, (2) identify differences in organismal thermal tolerance between red- and white- blooded notothenioids, (3) identify molecular mechanisms regulating changes in mitochondrial structure in icefishes. The results may establish channichthyid icefishes as a sentinel taxon for signaling the impact of global warming on the Southern Ocean. Broad impacts of this project will be realized by participation of high school biology teachers in field work through cooperation with the ARMADA project at the University of Rhode Island, as well as graduate education.", "east": -62.44, "geometry": "POINT(-63.445 -63.695)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -63.29, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Crockett, Elizabeth; O\u0027Brien, Kristin", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -64.1, "title": "Collaborative Research: Linkages among Mitochondrial Form, Function and Thermal Tolerance of Antarctic Notothenioid Fishes", "uid": "p0000483", "west": -64.45}, {"awards": "0839007 Near, Thomas", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "Genetic Sequence Data", "datasets": [{"dataset_uid": "000151", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Genetic Sequence Data", "url": "http://www.ncbi.nlm.nih.gov/genbank/"}], "date_created": "Fri, 22 Nov 2013 00:00:00 GMT", "description": "This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).\u003cbr/\u003e\u003cbr/\u003eThe teleost fish fauna in the waters surrounding Antarctica are completely dominated by a single clade of closely related species, the Notothenioidei. This clade offers an unprecedented opportunity to investigate the effects of deep time paleogeographic transformations and periods of global climate change on lineage diversification and facilitation of adaptive radiation. With over 100 species, the Antarctic notothenioid radiation has been the subject of intensive investigation of biochemical, physiological, and morphological adaptations associated with freezing avoidance in the subzero Southern Ocean marine habitats. However, broadly sampled time-calibrated phylogenetic hypotheses of notothenioids have not been used to examine patterns of adaptive radiation in this clade. The goals of this project are to develop an intensive phylogenomic scale dataset for 90 of the 124 recognized notothenioid species, and use this genomic resource to generate time-calibrated molecular phylogenetic trees. The results of pilot phylogenetic studies indicate a very exciting correlation of the initial diversification of notothenioids with the fragmentation of East Gondwana approximately 80 million years ago, and the origin of the Antarctic Clade adaptive radiation at a time of global cooling and formation of polar conditions in the Southern Ocean, approximately 35 million years ago. This project will provide research experiences for undergraduates, training for a graduate student, and support a post doctoral researcher. In addition the project will include three high school students from New Haven Public Schools for summer research internships.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Near, Thomas", "platforms": "Not provided", "repo": "NCBI GenBank", "repositories": "NCBI GenBank", "science_programs": null, "south": -90.0, "title": "Genomic Approaches to Resolving Phylogenies of Antarctic Notothenioid Fishes", "uid": "p0000497", "west": -180.0}, {"awards": "1043749 Rouse, Gregory", "bounds_geometry": null, "dataset_titles": "Expedition data of NBP1105", "datasets": [{"dataset_uid": "002659", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of NBP1105", "url": "https://www.rvdata.us/search/cruise/NBP1105"}], "date_created": "Mon, 24 Jun 2013 00:00:00 GMT", "description": "The west Antarctic Peninsula is warming rapidly, and continuing changes in the thermal regime will likely result in severe consequences for marine fauna, including potential extinction of strongly adapted stenotherms, and invasions from neighboring faunas. Initial impacts of climate change may result in changes in connectivity among populations of the same species. These changes may will be undetectable by direct observation, but may be assessed via genetic connectivity, i.e. differences in allele or haplotype frequencies among populations can be used to infer levels of gene flow. The proposed research will explore the role that the Scotia Arc plays in connecting populations from South America to Antarctica, a corridor identified as a likely entry route for invaders into Antarctica. It also will examine the way in which cryptic species may confound our knowledge of broad-scale distributions, and in doing so, make contributions towards understanding biodiversity and testing the paradigm of circumpolarity in Antarctica. The principal investigator will to collect multi-locus genetic data across \u0027species\u0027 from a broad suite of benthic marine invertebrate phyla, from multiple locations, in order to address hypotheses regarding speciation and connectivity, to estimate demographic population changes, and to identify the underlying processes that drive observed phylogeographic patterns. Comparative phylogeography is a particularly valuable approach because it enables the identification of long-term barriers and refugia common to groups of species and is consequently highly relevant to conservation planning. Moreover, this work will form a valuable baseline for detecting future changes in connectivity. The results of the research will be disseminated through peer-reviewed publications and presentations at conferences. In addition, the project will support the interdisciplinary training of a female graduate student, two undergraduate students, and host additional summer students through the STARS program at SIO, which helps minority students prepare for graduate school. This project will integrate research and education through conducting an interdisciplinary workshop that brings together Earth Science and Biology high school teachers. This workshop aims to assist teachers derive their own curricula uniting plate tectonics, ocean history and evolution, supporting a new high school earth sciences program. Information generated by this project will also directly feed into international efforts to design a series of Marine Protected Areas (MPAs) in Antarctica.", "east": null, "geometry": null, "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e CHEMICAL METERS/ANALYZERS \u003e FLUOROMETERS; IN SITU/LABORATORY INSTRUMENTS \u003e MAGNETIC/MOTION SENSORS \u003e GRAVIMETERS \u003e GRAVIMETERS; IN SITU/LABORATORY INSTRUMENTS \u003e RECORDERS/LOGGERS \u003e AWS; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e XBT; EARTH REMOTE SENSING INSTRUMENTS \u003e ACTIVE REMOTE SENSING \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ECHO SOUNDERS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e MBES; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e THERMOSALINOGRAPHS", "is_usap_dc": false, "keywords": "R/V NBP", "locations": null, "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Rouse, Gregory", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repo": "R2R", "repositories": "R2R", "science_programs": null, "south": null, "title": "Using molecular data to test connectivity and the circumpolar paradigm for Antarctic marine invertebrates", "uid": "p0000847", "west": null}, {"awards": "0739684 Hatcher, Patrick", "bounds_geometry": null, "dataset_titles": null, "datasets": null, "date_created": "Wed, 26 Sep 2012 00:00:00 GMT", "description": "This award supports a project to fully develop the analytical protocols needed to exploit a relatively new technique for the analysis of soluble organic matter in ice core samples. The technique couples Electrospray ionization to high resolution Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR-MS). Sample volume will be reduced and pre-concentration steps will be eliminated. Following method optimization a suite of ice core samples will be studied from several Antarctic and Greenland locations to address several hypothesis driven research questions. Preliminary results show that a vast record of relatively high molecular weight organic material exists in ice core samples and intriguing results from a few samples warrant further investigation. Several important questions related to developing a better understanding of the nature and paleo record of organic matter in ice cores will be addressed. These include developing a better understanding of the origin of nitrogen and sulfur isotopes in pre-industrial vs. modern samples, developing the methods to apply molecular biomarker techniques, routinely used by organic geochemists for sediment analyses, to the analysis of organic matter in ice cores, tracking the level of oxidation of homologous series of compounds and using them as a proxy for atmospheric oxidant levels in the past and determining whether or not high resolution FTICR mass spectral analysis can provide the ice core community with a robust method to analyze organic materials at the molecular level. The intellectual merit of this work is that this analytical method will provide a new understanding of the nature of organic matter in ice, possibly leading to the discovery of multitudes of molecular species indicative of global change processes whose abundances can be compared with other change proxies. The proposed studies are of an exploratory nature and potentially transformative for the field of ice core research and cryobiology. The broader impacts of these studies are that they should provide compelling evidence regarding organic matter sources, atmospheric processing and anthropogenic inputs to polar ice and how these have varied over time. The collaborative work proposed here will partner atmospheric chemistry/polar ice chemistry expertise with organic geochemistry expertise, resulting in significant contributions to both fields of study and significant advances in ice core analysis. Training of both graduate and undergraduate students will be a key component of the project and students will be involved in collaborative research using advanced analytical instrumentation, presentation of research results at national meetings, and will participate in manuscript preparation.", "east": null, "geometry": null, "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e SPECTROMETERS/RADIOMETERS \u003e MASS SPECTROMETERS", "is_usap_dc": false, "keywords": "Ice Core; Isotope; Organic Matter; Nitrogen; Sulfur; Not provided; LABORATORY; Mass Spectrometry; COMPUTERS; Molecular", "locations": null, "north": null, "nsf_funding_programs": "Antarctic Glaciology", "paleo_time": null, "persons": "Hatcher, Patrick; Grannas, Amanda", "platforms": "Not provided; OTHER \u003e MODELS \u003e COMPUTERS; OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repositories": null, "science_programs": null, "south": null, "title": "Collaborative Research: Molecular Level Characterization of Organic Matter in Ice Cores using High-resolution FTICR mass spectrometry", "uid": "p0000707", "west": null}, {"awards": "0741348 Torres, Joseph", "bounds_geometry": null, "dataset_titles": "Expedition data of NBP1002", "datasets": [{"dataset_uid": "002652", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of NBP1002", "url": "https://www.rvdata.us/search/cruise/NBP1002"}], "date_created": "Thu, 03 Mar 2011 00:00:00 GMT", "description": "Intellectual Merit: Pleuragramma antarcticum, the Antarctic silverfish, play a key role in the trophic pyramid of the Antarctic coastal ecosystem, acting as food for larger fishes, flying and non-flying seabirds, pinnipeds, and whales. In turn, they are predators on coastal euphausiids, including both Euphausia superba and crystallorophias. Historically, Pleuragramma have been an important food source for Ad\u00e9lie Penguins of the Western Antarctic Peninsula (WAP), but during the last decade Pleuragramma have disappeared from the Ad\u00e9lie diet. We suggest that Pleuragramma?s absence from the diets of top predators is linked to the declining sea ice canopy, which serves as a nursery for eggs and larvae during the austral spring. The research will investigate four hydrographic regimes over the WAP continental shelf with the following features: (1) persistent gyral flows that act to retain locally spawned larvae, (2) spring sea ice that has declined in recent years (3) the prevalence of adult silverfish, and (4) the presence of breeding Ad\u00e9lie penguins whose diets vary in the proportions of silverfish consumed. The research will evaluate the importance of local reproduction versus larval advection, and the extent to which populations in the subregions of study are genetically distinct, via analysis of population structure, otolith microchemistry and molecular genetics of fish. The Pleuragramma data will be compared with penguin diet samples taken synoptically. \u003cbr/\u003e\u003cbr/\u003eBroader Impacts: The proposed research brings together an international group of scientists with highly complimentary suites of skills to address the fate of Pleuragramma on the WAP shelf. Graduate students will use the data acquired as part of their Ph.D research, and will receive cross-training in ornithological field techniques, molecular genetic methods and otolith isotope chemistry. The PIs will work actively with the St. Petersburg Times to produce a blog in real time with pictures and text, which will be used to interact with local schools while we are at sea and after our return. The investigators also will collaborate with the COSEE center at USF and at local schools and museums to disseminate results to the K-12 community throughout the region.", "east": null, "geometry": null, "instruments": "EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS", "is_usap_dc": false, "keywords": "R/V NBP", "locations": null, "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Torres, Joseph", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repo": "R2R", "repositories": "R2R", "science_programs": null, "south": null, "title": "Collaborative research: Possible climate-induced change in the distribution of Pleuragramma antarcticum on the Western Antarctic Peninsula shelf", "uid": "p0000842", "west": null}, {"awards": "0635470 Detrich, H. William", "bounds_geometry": "POLYGON((-67.41667 -61.2,-66.27667 -61.2,-65.13667 -61.2,-63.99667 -61.2,-62.85667 -61.2,-61.71667 -61.2,-60.57667 -61.2,-59.43667 -61.2,-58.29667 -61.2,-57.15667 -61.2,-56.01667 -61.2,-56.01667 -61.71,-56.01667 -62.22,-56.01667 -62.73,-56.01667 -63.24,-56.01667 -63.75,-56.01667 -64.26,-56.01667 -64.77,-56.01667 -65.28,-56.01667 -65.79,-56.01667 -66.3,-57.15667 -66.3,-58.29667 -66.3,-59.43667 -66.3,-60.57667 -66.3,-61.71667 -66.3,-62.85667 -66.3,-63.99667 -66.3,-65.13667 -66.3,-66.27667 -66.3,-67.41667 -66.3,-67.41667 -65.79,-67.41667 -65.28,-67.41667 -64.77,-67.41667 -64.26,-67.41667 -63.75,-67.41667 -63.24,-67.41667 -62.73,-67.41667 -62.22,-67.41667 -61.71,-67.41667 -61.2))", "dataset_titles": "Expedition Data; Expedition data of LMG1003; Expedition data of LMG1004; Sequence data", "datasets": [{"dataset_uid": "002684", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG1003", "url": "https://www.rvdata.us/search/cruise/LMG1003"}, {"dataset_uid": "000133", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Sequence data", "url": "http://www.ncbi.nlm.nih.gov/genbank/"}, {"dataset_uid": "001508", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0807"}, {"dataset_uid": "001509", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0806"}, {"dataset_uid": "002685", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG1004", "url": "https://www.rvdata.us/search/cruise/LMG1004"}], "date_created": "Mon, 06 Sep 2010 00:00:00 GMT", "description": "Abstract\u003cbr/\u003e\u003cbr/\u003eSince the advent of Antarctic continental glaciation, the opening of the Drake Passage between South America and the Antarctic Peninsula, and the onset of cooling of the Southern Ocean ~38-25 million years ago, evolution of the Antarctic marine biota has been driven by the development of extreme cold temperatures. Because they live at very low and stable temperatures, Antarctic fishes of the suborder Nototheniodei are particularly attractive as models for understanding the mechanisms of biomolecular cold adaptation, or the compensatory restructuring of biochemical and physiological systems to preserve biological function in cold thermal regimes. Two interrelated and potentially co-evolved systems, the tubulins that form microtubules and the chaperonin-containing TCP1 (t-complex protein-1) complex (CCT) that assists the folding of tubulins, provide an unparalleled opportunity to elucidate these mechanisms. This research will yield new and important knowledge regarding: 1) cold adaptation of microtubule assembly and of chaperonin function; and 2) the co-evolutionary origin of tubulin-binding specificity by CCT. The first objective of this proposal is to determine the contributions of five novel amino acid substitutions found in Antarctic fish beta-tubulins to microtubule assembly at cold temperature. The second objective is to establish a chaperonin folding system in vitro using CCT purified from testis tissue of Antarctic fishes and to evaluate its thermal properties and mechanism. The third objective is to evaluate, through phylogenetically controlled contrasts, the hypothesis that CCT and its tubulin substrates from Antarctic fishes have co-evolved to function at cold temperatures. The broader impacts of this proposal include introduction of graduate and REU undergraduate students of Northeastern University to state-of-the-art biochemical, cellular, and molecular-biological research relevant to ecological and environmental issues of the Antarctic marine ecosystem. Because much of the research on the biogenesis and function of cold-adapted proteins will be performed in the field at Palmer Station, these students will gain invaluable experience in the practical considerations of expeditionary biological science. The research also will increase knowledge about molecular cold adaptation in one of the Earth\u0027s extreme environments, and hence is relevant to the formulation of refined hypotheses regarding potential extraterrestrial life on Mars or Europa. The cold-functioning chaperonin protein folding system will be of great value to the biopharmaceutical and biotechnological industries for use in folding insoluble proteins.", "east": -56.01667, "geometry": "POINT(-61.71667 -63.75)", "instruments": "EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ADCP", "is_usap_dc": true, "keywords": "R/V LMG", "locations": null, "north": -61.2, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Detrich, H. William", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "R2R", "repositories": "NCBI GenBank; R2R", "science_programs": null, "south": -66.3, "title": "Protein Folding and Function at Cold Temperature: Co-Evolution of the Chaperonin CCT and Tubulins from Antarctic Fishes", "uid": "p0000470", "west": -67.41667}, {"awards": "0801392 Swanson, Brian", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "Ice Nucleation by Marine Psychrophiles", "datasets": [{"dataset_uid": "600087", "doi": "10.15784/600087", "keywords": "Biota; Microbiology; Oceans; Raman Spectroscopy; Sea Ice; Sea Surface; Southern Ocean", "people": "Swanson, Brian", "repository": "USAP-DC", "science_program": null, "title": "Ice Nucleation by Marine Psychrophiles", "url": "https://www.usap-dc.org/view/dataset/600087"}], "date_created": "Sat, 26 Jun 2010 00:00:00 GMT", "description": "The primary objective of this research is to investigate polar marine psychrophilic bacteria for their potential to nucleate ice using a combination of microbiological, molecular biological and atmospheric science approaches in the laboratory. Very little is known about how psychrophiles interact and cope with ice or their adaptations to conditions of extreme cold and salinity. This work will involve a series of laboratory experiments using a novel freeze-tube technique for assaying freezing spectra which will provide quantitative information on: (i) the temperature-dependent freezing rates for heterogeneously frozen droplets containing sea-ice bacteria, (ii) the proportional occurrence of ice-nucleation activity versus anti-freeze activity among sea-ice bacterial isolates and (iii) the temperature-dependent freezing rates of bacteria with ice-nucleation activity grown at a range of temperatures and salinities. The compound(s) responsible for the observed activity will be identified, which is an essential step towards the development of an in-situ bacterial ice-nucleation detection assay that can be applied in the field to Antarctic water and cloud samples.\u003cbr/\u003e One of the goals of this work is to better understand survival and cold adaptation processes of polar marine bacteria confronted with freezing conditions in sea ice. Since sea ice strongly impacts polar, as well as the global climates, this research is of significant interest because it will also provide data for accessing the importance of bacterial ice nucleation in the formation of sea ice. These measurements of ice-nucleation rates will be the first high-resolution measurements for psychrophilic marine bacteria. Another goal is to better understand the impact of bacterial ice initiation processes in polar clouds by making high-resolution measurements of nucleation rates for cloud bacteria found over Arctic and Antarctic regions. Initial measurements indicate these bacteria nucleate ice at warmer temperatures and the effect in polar regions may be quite important, since ice can strongly impact cloud dynamics, cloud radiative properties, precipitation formation, and cloud chemistry. If these initial measurements are confirmed, the data collected here will be important for improving the understanding of polar cloud processes and models. A third goal is to better understand the molecular basis of marine bacterial ice nucleation by characterizing the ice-nucleation compound and comparing it with those of known plant-derived ice-nucleating bacteria, which are the only ice-nucleating bacteria examined in detail to date. The proposed activity will support the beginning academic career of a post-doctoral researcher and will serve as the basis for several undergraduate student laboratory projects. Results from this research will be widely published in various scientific journals and outreach venues.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Swanson, Brian", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -90.0, "title": "Ice Nucleation by Marine Psychrophiles", "uid": "p0000195", "west": -180.0}, {"awards": "9909055 Sidell, Bruce", "bounds_geometry": null, "dataset_titles": "Expedition Data; Expedition data of LMG0105", "datasets": [{"dataset_uid": "002693", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0105", "url": "https://www.rvdata.us/search/cruise/LMG0105"}, {"dataset_uid": "001869", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0105"}], "date_created": "Tue, 04 May 2010 00:00:00 GMT", "description": "The suborder Notothenoidei is the dominant fish group of the Southern Ocean surrounding Antarctica, both in terms of number of species and biomass. For about fourteen million years, these highly successful fish evolved under stable thermal conditions that result in body temperatures of about zero degrees centigrade throughout their life histories. Evolution this cold environment has led to unusual physiological and biochemical characteristics. In some cases, the characteristics contribute to overcoming constraints of cold temperature on biological processes. In other instances, mutations that probably would have been lethal in warmer, less oxygen-rich environments than the Southern Ocean have been retained in Antarctic fishes. This research project focuses on three major objectives that exploit these unusual conditions to identify mechanisms compatible with normal cellular function at cold temperature and to gain unique insights into the physiological roles of key intracellular proteins. The three lines of study proposed are the molecular basis for the failure of the myoglobin encoding gene to be expressed in certain Antarctic notothenioid fishes, the basis of the substrate specificity of the enzyme fatty acyl-CoA synthetase that is involved in the catabolism of fatty acids, and the functional roles played by different isoforms of creatine phosphokinase in locomotory muscle of Antarctic fish. Results from this study will not only provide insight into the evolutionary biology of the Antarctic notothenioid fishes, but will elucidate important general principles that are applicable to widely different taxa beyond the Antarctic.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": false, "keywords": "R/V LMG", "locations": null, "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Sidell, Bruce", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "R2R", "repositories": "R2R", "science_programs": null, "south": null, "title": "Proteins of Oxygen-Binding and Energy Metabolism in Muscles of Antarctic Fishes: Evolutionary Adjustments to Life at Cold Temperature", "uid": "p0000863", "west": null}, {"awards": "9527255 Mopper, Kenneth", "bounds_geometry": null, "dataset_titles": "Expedition data of LMG9809", "datasets": [{"dataset_uid": "002719", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG9809", "url": "https://www.rvdata.us/search/cruise/LMG9809"}], "date_created": "Tue, 04 May 2010 00:00:00 GMT", "description": "ACT K. Mopper \u0026 D. Kieber OPP 9527255 \u0026 OPP 9527314 PHOTOCHEMICAL AND OPTICAL PROPERTIES OF ANTARCTIC WATERS IN RESPONSE TO CHANGING UV-B FLUXES The decrease in stratospheric ozone over the Antarctic results in an increase in the UV-B flux in the euphotic zone. The increase leads to cellular damage to aquatic organisms, as documented by photo-inhibition and decreased productivity. Cellular damage can occur either intracellularly, or externally at the cell surface from biomolecular reactions with externally-generated reactive transients. The extent of this extracellular damage will depend on the photochemistry of the seawater surrounding the cell. Until recently, nothing was known about the type of photochemical processes, rates, and steady state concentrations of transients in Antarctic waters. It is proposed that field experiments be performed which will allow the construction of predictive models of photochemical production rates in surface waters and with depth. These studies will involve further quantum yield measurementts, development of a sensitive underwater actinometer system, and use of a new underwater multichannel photometer. The model will allow the prediction of the impact of varying levels of UV-B on the photoproduction and steady state concentration of several key reactive transient species in the upper water column. In addition to this effort, experiments will also be performed to study the photodegradation of dissolved organic matter and to determine whether biologically utilizable substrates that are formed photochemically can enhance secondary productivity in Antarctic waters.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": false, "keywords": "R/V LMG", "locations": null, "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Mopper, Kenneth", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "R2R", "repositories": "R2R", "science_programs": null, "south": null, "title": "Collaborative Research: Photochemical and Optical Properties of Antarctic Waters in Response to Changing UV-B Fluxes", "uid": "p0000870", "west": null}, {"awards": "0125890 Sidell, Bruce", "bounds_geometry": "POLYGON((-68.1413 -52.6755,-67.47503 -52.6755,-66.80876 -52.6755,-66.14249 -52.6755,-65.47622 -52.6755,-64.80995 -52.6755,-64.14368 -52.6755,-63.47741 -52.6755,-62.81114 -52.6755,-62.14487 -52.6755,-61.4786 -52.6755,-61.4786 -53.8957,-61.4786 -55.1159,-61.4786 -56.3361,-61.4786 -57.5563,-61.4786 -58.7765,-61.4786 -59.9967,-61.4786 -61.2169,-61.4786 -62.4371,-61.4786 -63.6573,-61.4786 -64.8775,-62.14487 -64.8775,-62.81114 -64.8775,-63.47741 -64.8775,-64.14368 -64.8775,-64.80995 -64.8775,-65.47622 -64.8775,-66.14249 -64.8775,-66.80876 -64.8775,-67.47503 -64.8775,-68.1413 -64.8775,-68.1413 -63.6573,-68.1413 -62.4371,-68.1413 -61.2169,-68.1413 -59.9967,-68.1413 -58.7765,-68.1413 -57.5563,-68.1413 -56.3361,-68.1413 -55.1159,-68.1413 -53.8957,-68.1413 -52.6755))", "dataset_titles": "Expedition Data; Expedition data of LMG0304; Expedition data of LMG0304A", "datasets": [{"dataset_uid": "002708", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0304A", "url": "https://www.rvdata.us/search/cruise/LMG0304A"}, {"dataset_uid": "001596", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0506"}, {"dataset_uid": "001597", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0505"}, {"dataset_uid": "001704", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0304"}, {"dataset_uid": "002707", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0304A", "url": "https://www.rvdata.us/search/cruise/LMG0304A"}, {"dataset_uid": "002706", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0304", "url": "https://www.rvdata.us/search/cruise/LMG0304"}], "date_created": "Tue, 04 May 2010 00:00:00 GMT", "description": "Notothenioid fishes that dominate the fish fauna surrounding Antarctica have been evolving for 10-14 million years at a nearly constant body temperature of ~0C throughout their life histories. As a result, this group of animals is uniquely suited to studies aimed at understanding and identifying features of physiology and biochemistry that result from the process of evolution at cold body temperature. This project has three major objectives aimed at examining adaptations for life in cold environments: \u003cbr/\u003e\u003cbr/\u003e1. Identify the amino acid substitutions in the fatty acid-binding pocket of fatty acyl CoA synthetase (FACS) that explain its substrate specificity. Fatty acids are a major fuel of energy metabolism in Antarctic fishes. FACS catalyzes the condensation of CoASH and fatty acids to fatty acyl CoA esters, a step required for subsequent metabolism of these important compounds. This research may permit us to resolve the specific amino acid substitutions that explain both substrate specificity and preservation of catalytic rate of notothenioid FACS at cold physiological temperatures.\u003cbr/\u003e\u003cbr/\u003e2. Produce a rigorous biochemical and biophysical characterization of the intracellular calcium-binding protein, parvalbumin, from white axial musculature of Antarctic fishes. Parvalbumin plays a pivotal role in facilitating the relaxation phase of fast-contracting muscles and is a likely site of strong selective pressure. Preliminary data strongly indicate that the protein from Antarctic fishes has been modified to ensure function at cold temperature. A suite of physical techniques will be used to determine dissociation constants of Antarctic fish parvalbumins for calcium and magnesium and unidirectional rate constants of ion-dissociation from the protein. Full-length cDNA clones for Antarctic fish parvalbumin(s) will permit deduction of primary amino acid sequence These data will yield insight into structural elements that permit the protein from notothenioid fishes to function at very cold body temperature.\u003cbr/\u003e\u003cbr/\u003e3. Conduct a broad survey of the pattern of cardiac myoglobin expression in the Suborder Notothenoidei. Previous work has indicated a variable pattern of presence or absence of the intracellular oxygen-binding protein, myoglobin (Mb), in hearts of one family of Antarctic notothenioid fishes (Channichthyidae; icefishes). Because Mb is of physiological value in species that express the protein, the observed pattern of interspecific expression has been attributed to unusually low niche competition in the Southern Ocean. This leads to the prediction that similar loss of cardiac Mb should be observed in other notothenioid taxa. This part of the project will survey for the presence and absence of cardiac Mb in as many notothenioid species as possible and, if Mb-lacking species are detected, will extend analyses to determine the mechanism(s) responsible for loss of its expression using molecular biological techniques.", "east": -61.4786, "geometry": "POINT(-64.80995 -58.7765)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PRESSURE/HEIGHT METERS \u003e PRESSURE SENSORS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ADCP", "is_usap_dc": false, "keywords": "R/V LMG", "locations": null, "north": -52.6755, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Sidell, Bruce; Detrich, H. William", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "R2R", "repositories": "R2R", "science_programs": null, "south": -64.8775, "title": "Cold Body Temperature as an Evolutionary Shaping force in the Physiology of Antarctic Fishes", "uid": "p0000241", "west": -68.1413}, {"awards": "9714299 Caron, David", "bounds_geometry": "POLYGON((-179.9984 -43.56676,-143.99865 -43.56676,-107.9989 -43.56676,-71.99915 -43.56676,-35.9994 -43.56676,0.000349999999997 -43.56676,36.0001 -43.56676,71.99985 -43.56676,107.9996 -43.56676,143.99935 -43.56676,179.9991 -43.56676,179.9991 -47.057693,179.9991 -50.548626,179.9991 -54.039559,179.9991 -57.530492,179.9991 -61.021425,179.9991 -64.512358,179.9991 -68.003291,179.9991 -71.494224,179.9991 -74.985157,179.9991 -78.47609,143.99935 -78.47609,107.9996 -78.47609,71.99985 -78.47609,36.0001 -78.47609,0.000349999999997 -78.47609,-35.9994 -78.47609,-71.99915 -78.47609,-107.9989 -78.47609,-143.99865 -78.47609,-179.9984 -78.47609,-179.9984 -74.985157,-179.9984 -71.494224,-179.9984 -68.003291,-179.9984 -64.512358,-179.9984 -61.021425,-179.9984 -57.530492,-179.9984 -54.039559,-179.9984 -50.548626,-179.9984 -47.057693,-179.9984 -43.56676))", "dataset_titles": "Expedition Data", "datasets": [{"dataset_uid": "002003", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/NBP9901"}], "date_created": "Tue, 04 May 2010 00:00:00 GMT", "description": "*** Caron 9714299 The analysis of microbial biodiversity of extreme environments is difficult because traditional methods for examining diversity are often ineffective for assessing species richness within these communities. Additional difficulties arise due to the difficulties of recreating and maintaining pertinent environmental features during sample collection and procession. This study focuses on the protistan assemblages (algae and protozoa) in the sea ice, sediment and ocean environments of the Ross Sea, Antarctica. The identification of protistan species in natural assemblages traditionally has entailed direct microscopical analyses as well as enrichment and culture techniques for assessing biodiversity. Determination of diversity for these assemblages in therefore susceptible to biases as a consequence of sampling, enrichment and culture, as well as selective losses due to sample preservation and concentration for microscopy. The goals of this project are: (1) to develop and apply molecular biological approaches to assess species diversity of small protists (algae and protozoa smaller than 100 micrometers) in ocean water, sea ice and sediment environments and (2) to obtain baseline physiological information on the growth rates, feeding rates and growth efficiencies of cultured protozoa under pertinent temperature regimes. Molecular biological studies will involve the use of PCR-based protocols to examine small subunit ribosomal RNA gene (srDNA) diversity. Approaches and techniques developed will be applicable to any other water body or sediment and would provide a means to examine the representativeness of protistan cultures in extant culture collections. ***", "east": 179.9991, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": false, "keywords": "R/V NBP", "locations": null, "north": -43.56676, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Caron, David; Jeffries, Martin", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repo": "R2R", "repositories": "R2R", "science_programs": null, "south": -78.47609, "title": "LEXEN: Protistan Biodiversity in Antarctic Marine Ecosystems: Molecular Biological and Traditional Approaches", "uid": "p0000625", "west": -179.9984}, {"awards": "0089451 Detrich, H. William", "bounds_geometry": "POLYGON((-70.907 -52.353,-69.8619 -52.353,-68.8168 -52.353,-67.7717 -52.353,-66.7266 -52.353,-65.6815 -52.353,-64.6364 -52.353,-63.5913 -52.353,-62.5462 -52.353,-61.5011 -52.353,-60.456 -52.353,-60.456 -53.64334,-60.456 -54.93368,-60.456 -56.22402,-60.456 -57.51436,-60.456 -58.8047,-60.456 -60.09504,-60.456 -61.38538,-60.456 -62.67572,-60.456 -63.96606,-60.456 -65.2564,-61.5011 -65.2564,-62.5462 -65.2564,-63.5913 -65.2564,-64.6364 -65.2564,-65.6815 -65.2564,-66.7266 -65.2564,-67.7717 -65.2564,-68.8168 -65.2564,-69.8619 -65.2564,-70.907 -65.2564,-70.907 -63.96606,-70.907 -62.67572,-70.907 -61.38538,-70.907 -60.09504,-70.907 -58.8047,-70.907 -57.51436,-70.907 -56.22402,-70.907 -54.93368,-70.907 -53.64334,-70.907 -52.353))", "dataset_titles": "Expedition Data; Expedition data of LMG0304A", "datasets": [{"dataset_uid": "001869", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0105"}, {"dataset_uid": "001704", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0304"}, {"dataset_uid": "002707", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0304A", "url": "https://www.rvdata.us/search/cruise/LMG0304A"}], "date_created": "Tue, 04 May 2010 00:00:00 GMT", "description": "Abstract\u003cbr/\u003eOPP-0089451\u003cbr/\u003eP.I. William Detrich\u003cbr/\u003e\u003cbr/\u003e As the Southern Ocean cooled during the past 25 million years, the fishes of Antarctic coastal waters evolved biochemical and physiological adaptations that maintain essential cellular processes such as cytoskeletal function and gene transcription. Their microtubules, for example, assemble and function at body temperatures (-1.8 to +1 oC) well below those of homeotherms and temperate poikilotherms. The long range goals of the proposed research are to determine, at the molecular level, the adaptations that enhance the assembly of microtubules, the function of kinesin motors, and the expression of globin and tubulin genes. The specific objectives are three: 1) to determine the primary sequence changes and posttranslational modifications that contribute to the efficient polymerization of Antarctic fish tubulins at low temperatures; 2) to evaluate the biochemical adaptations required for efficient function of the brain kinesin motor of Antarctic fishes at low temperatures; and 3) to characterize the structure, organization, and promoter-driven expression of globin and tubulin genes from an Antarctic rockcod (Notothenia coriiceps) and a temperate congener (N. angustata). Brain tubulins from Antarctic fishes differ from those of temperate and warm-blooded vertebrates both in unusual primary sequence substitutions (located primarily in lateral loops and the cores of tubulin monomers) and in posttranslational C-terminal glutamylation. Potential primary sequence adaptations of the Antarctic fish tubulins will be tested directly by production of wild-type and site directed tubulin mutants for functional analysis in vitro. The capacity of mutated and wild-type fish tubulins to form \"cold-stable\" microtubules will be determined by measurement of their critical concentrations for assembly and by analysis of their dynamics by video-enhanced microscopy. Three unusual substitutions in the kinesin motor domain of Chionodraco rastrospinosus may enhance mechanochemical activity at low temperature by modifying the binding of ATP and/or the velocity of the motor. To test the functional significance of these changes, the fish residues will be converted individually, and in concert, to those found in mammalian brain kinesin. Reciprocal substitutions will be introduced into the framework of the mammalian kinesin motor domain. After production in Escherichia coli and purification, the functional performance of the mutant motor domains will be evaluated by measurement of the temperature dependence of their ATPase and motility activities. Molecular adaptation of gene expression in N. coriiceps will be analyzed using an a-globin/b-globin gene pair and an a-tubulin gene cluster. Structural features of N. coriiceps globin and tubulin gene regulatory sequences (promoters and enhancers) that support efficient expression will be assessed by transient transfection assay of promoter/luciferase reporter plasmid constructs in inducible erythrocytic and neuronal model cell systems followed by assay of luciferase reporter activity. Together, these studies should reveal the molecular adaptations of Antarctic fishes that maintain efficient cytoskeletal assembly, mechanochemical motor function, and gene expression at low temperatures. In the broadest sense, this research program should advance the molecular understanding of the poikilothermic mode of life.", "east": -60.456, "geometry": "POINT(-65.6815 -58.8047)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; IN SITU/LABORATORY INSTRUMENTS \u003e PRESSURE/HEIGHT METERS \u003e PRESSURE SENSORS; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ADCP", "is_usap_dc": false, "keywords": "R/V LMG", "locations": null, "north": -52.353, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Sidell, Bruce; Detrich, H. William", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "R2R", "repositories": "R2R", "science_programs": null, "south": -65.2564, "title": "Structure, Function, and Expression of Tubulins, Globins, and Microtubule-Dependent Motors from Cold-Adapted Antarctic Fishes", "uid": "p0000591", "west": -70.907}, {"awards": "0132032 Detrich, H. William", "bounds_geometry": "POLYGON((-68.84315 -42.87167,-61.576321 -42.87167,-54.309492 -42.87167,-47.042663 -42.87167,-39.775834 -42.87167,-32.509005 -42.87167,-25.242176 -42.87167,-17.975347 -42.87167,-10.708518 -42.87167,-3.441689 -42.87167,3.82514 -42.87167,3.82514 -44.482708,3.82514 -46.093746,3.82514 -47.704784,3.82514 -49.315822,3.82514 -50.92686,3.82514 -52.537898,3.82514 -54.148936,3.82514 -55.759974,3.82514 -57.371012,3.82514 -58.98205,-3.441689 -58.98205,-10.708518 -58.98205,-17.975347 -58.98205,-25.242176 -58.98205,-32.509005 -58.98205,-39.775834 -58.98205,-47.042663 -58.98205,-54.309492 -58.98205,-61.576321 -58.98205,-68.84315 -58.98205,-68.84315 -57.371012,-68.84315 -55.759974,-68.84315 -54.148936,-68.84315 -52.537898,-68.84315 -50.92686,-68.84315 -49.315822,-68.84315 -47.704784,-68.84315 -46.093746,-68.84315 -44.482708,-68.84315 -42.87167))", "dataset_titles": "Expedition Data", "datasets": [{"dataset_uid": "001655", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/NBP0404"}], "date_created": "Tue, 04 May 2010 00:00:00 GMT", "description": "Notothenioid fish are a major group of fish in the Southern Ocean. The ancestral notothenioid fish stock of Antarctica probably arose as a sluggish, bottom-dwelling perciform species that evolved some 40-60 million years ago in the then temperate shelf waters of the Antarctic continent. The grounding of the ice sheet on the continental shelf and changing trophic conditions may have eliminated the taxonomically diverse late Eocene fauna and initiated the original diversification of notothenioids. On the High Antarctic shelf, notothenioids today dominate the ichthyofauna in terms of species diversity, abundance and biomass, the latter two at levels of 90-95%. Since the International Geophysical Year of 1957-58, fish biologists from the Antarctic Treaty nations have made impressive progress in understanding the notothenioid ichthyofauna of the cold Antarctic marine ecosystem. However, integration of this work into the broader marine context has been limited, largely due to lack of access to, and analysis of, specimens of Sub-Antarctic notothenioid fishes. Sub-Antarctic fishes of the notothenioid suborder are critical for a complete understanding of the evolution, population dynamics, eco-physiology, and eco-biochemistry of their Antarctic relatives. This project will support an international, collaborative research cruise to collect and study fish indigenous to sub-antarctic habitats. The topics included in the research plans of the international team of researchers includes Systematics and Evolutionary Studies; Life History Strategies and Population Dynamics; Physiological, Biochemical, and Molecular Biological Investigations of Major Organ and Tissue Systems; Genomic Resources for the Sub-Antarctic Notothenioids; and Ecological Studies of Transitional Benthic Invertebrates. In a world that is experiencing changes in global climate, the loss of biological diversity, and the depletion of marine fisheries, the Antarctic, Sub-Antarctic, and their biota offer compelling natural laboratories for understanding the evolutionary impacts of these processes. The proposed work will contribute to development of a baseline understanding of these sensitive ecosystems, one against which future changes in species distribution and survival may be evaluated judiciously.", "east": 3.82514, "geometry": "POINT(-32.509005 -50.92686)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; IN SITU/LABORATORY INSTRUMENTS \u003e MAGNETIC/MOTION SENSORS \u003e GRAVIMETERS \u003e GRAVIMETERS; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ADCP", "is_usap_dc": false, "keywords": "R/V NBP", "locations": null, "north": -42.87167, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Detrich, H. William", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repo": "R2R", "repositories": "R2R", "science_programs": null, "south": -58.98205, "title": "International Collaborative Expedition to Collect and Study Fish Indigenous to Sub-Antarctic Habitats", "uid": "p0000584", "west": -68.84315}, {"awards": "9910164 Scheltema, Rudolf", "bounds_geometry": "POLYGON((-70.557 -52.351,-68.9316 -52.351,-67.3062 -52.351,-65.6808 -52.351,-64.0554 -52.351,-62.43 -52.351,-60.8046 -52.351,-59.1792 -52.351,-57.5538 -52.351,-55.9284 -52.351,-54.303 -52.351,-54.303 -53.60557,-54.303 -54.86014,-54.303 -56.11471,-54.303 -57.36928,-54.303 -58.62385,-54.303 -59.87842,-54.303 -61.13299,-54.303 -62.38756,-54.303 -63.64213,-54.303 -64.8967,-55.9284 -64.8967,-57.5538 -64.8967,-59.1792 -64.8967,-60.8046 -64.8967,-62.43 -64.8967,-64.0554 -64.8967,-65.6808 -64.8967,-67.3062 -64.8967,-68.9316 -64.8967,-70.557 -64.8967,-70.557 -63.64213,-70.557 -62.38756,-70.557 -61.13299,-70.557 -59.87842,-70.557 -58.62385,-70.557 -57.36928,-70.557 -56.11471,-70.557 -54.86014,-70.557 -53.60557,-70.557 -52.351))", "dataset_titles": "Expedition Data", "datasets": [{"dataset_uid": "001846", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0109"}], "date_created": "Tue, 04 May 2010 00:00:00 GMT", "description": "Because of the extreme isolation of Antarctica since the early Oligocene one can expect to encounter a unique invertebrate fauna with a high degree of endemism. Yet, some benthic taxa include from 20 to \u003e50 percent non-endemic species. To account for such species it has been proposed that an intermittent reciprocal exchange must occur between the antiboreal populations of South America and the Antarctic continent. One possible means by which the geographical distribution can be maintained and genetic exchange may be accomplished is by the passive dispersal of planktonic larvae. To show that such dispersal is actually accomplished it must be demonstrated that (1) larvae of sublittoral species actually are found within the Drake passage and that such larvae belong to species that occur both in the antiboreal South American and Antarctic faunas and (2) that a hydrographic mechanism exists that can explain how the passive transport of larvae may occur between the two continents. The proposed research will address these two requirements by making transects of plankton samples across the Drake passage and by examining the possibility of cross frontal exchange of larvae at the subantarctic and polar fronts of the Antarctic circumpolar current as well as the possible transport of larvae in mesoscale rings. The outcome may suggest species that in the future may profitably be examined using molecular techniques, comparing individuals from bottom populations of South America and Antarctica. The study necessarily must be of a very preliminary nature since the occurrence of planktonic larvae of sublittoral benthic species in the Drake Passage has never before been examined.", "east": -54.303, "geometry": "POINT(-62.43 -58.62385)", "instruments": null, "is_usap_dc": false, "keywords": "R/V LMG", "locations": null, "north": -52.351, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Scheltema, Rudolf; Veit, Richard", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "R2R", "repositories": "R2R", "science_programs": null, "south": -64.8967, "title": "SGER: Dispersal of Planktonic Invertebrate Larvae and the Biogeography of the Antarctic Benthos", "uid": "p0000602", "west": -70.557}, {"awards": "0632250 Cary, Stephen", "bounds_geometry": "POLYGON((-38.5 -72.6,-23.963 -72.6,-9.426 -72.6,5.111 -72.6,19.648 -72.6,34.185 -72.6,48.722 -72.6,63.259 -72.6,77.796 -72.6,92.333 -72.6,106.87 -72.6,106.87 -73.185,106.87 -73.77,106.87 -74.355,106.87 -74.94,106.87 -75.525,106.87 -76.11,106.87 -76.695,106.87 -77.28,106.87 -77.865,106.87 -78.45,92.333 -78.45,77.796 -78.45,63.259 -78.45,48.722 -78.45,34.185 -78.45,19.648 -78.45,5.111 -78.45,-9.426 -78.45,-23.963 -78.45,-38.5 -78.45,-38.5 -77.865,-38.5 -77.28,-38.5 -76.695,-38.5 -76.11,-38.5 -75.525,-38.5 -74.94,-38.5 -74.355,-38.5 -73.77,-38.5 -73.185,-38.5 -72.6))", "dataset_titles": "Metagenomic Data Lake Vostok Microbial Community", "datasets": [{"dataset_uid": "000136", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Metagenomic Data Lake Vostok Microbial Community", "url": "http://www.ncbi.nlm.nih.gov/genbank/"}], "date_created": "Fri, 30 Apr 2010 00:00:00 GMT", "description": "This project brings together researchers with expertise in molecular microbial ecology, Antarctic and deep sea environments, and metagenomics to address the overarching question: how do ecosystems dominated by microorganisms adapt to conditions of continuous cold and dark over evolutionarily and geologically relevant time scales? Lake Vostok, buried for at least 15 million years beneath approximately 4 km of ice that has prevented any communication with the external environment for as much as 1.5 million years, is an ideal system to study this question. Water from the lake that has frozen on to the bottom of the ice sheet (accretion ice) is available for study. Several studies have indicated the presence of low abundance, but detectable microbial communities in the accretion ice. Our central hypothesis maintains that Lake Vostok microbes are specifically adapted to life in conditions of extreme cold, dark, and oligotrophy and that signatures of those adaptations can be observed in their genome sequences at the gene, organism, and community levels. To address this hypothesis, we propose to characterize the metagenome (i.e. the genomes of all members of the community) of the accretion ice. using whole genome amplification (WGA), which can provide micrograms of unbiased metagenomic DNA from only a few cells. The results of this project have relevance to evolutionary biology and ecology, subglacial Antarctic lake exploration, biotechnology, and astrobiology. The project directly addresses priorities and themes in the International Polar Year at the national and international levels. A legacy of DNA sequence data and the metagenomic library will be created and maintained. Press releases and a publicly available web page will facilitate communication with the public. K-12 outreach will be the focus of a new, two-tiered program targeting the 7th grade classroom and on site visits to the Joint Genome Institute Production Sequencing Facility by high school juniors and seniors and community college level students. Minority undergraduate researchers will be recruited for research on this project, and support and training are provided to two graduate students, a postdoctoral scholar, and a technician.", "east": 106.87, "geometry": "POINT(34.185 -75.525)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -72.6, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Cary, Stephen", "platforms": "Not provided", "repo": "NCBI GenBank", "repositories": "NCBI GenBank", "science_programs": null, "south": -78.45, "title": "IPY: Collaborative Research: A Metagenomic Investigation of Adaptation to Prolonged Cold and Dark Conditions of the Lake Vostok Microbial Community", "uid": "p0000201", "west": -38.5}, {"awards": "0808947 Hofmann, Gretchen", "bounds_geometry": "POLYGON((-180 -70,-177 -70,-174 -70,-171 -70,-168 -70,-165 -70,-162 -70,-159 -70,-156 -70,-153 -70,-150 -70,-150 -70.7,-150 -71.4,-150 -72.1,-150 -72.8,-150 -73.5,-150 -74.2,-150 -74.9,-150 -75.6,-150 -76.3,-150 -77,-153 -77,-156 -77,-159 -77,-162 -77,-165 -77,-168 -77,-171 -77,-174 -77,-177 -77,180 -77,178 -77,176 -77,174 -77,172 -77,170 -77,168 -77,166 -77,164 -77,162 -77,160 -77,160 -76.3,160 -75.6,160 -74.9,160 -74.2,160 -73.5,160 -72.8,160 -72.1,160 -71.4,160 -70.7,160 -70,162 -70,164 -70,166 -70,168 -70,170 -70,172 -70,174 -70,176 -70,178 -70,-180 -70))", "dataset_titles": "Science of Opportunity: A SGER proposal to support the development of genomic resources for Antarctic pteropods", "datasets": [{"dataset_uid": "600088", "doi": "10.15784/600088", "keywords": "Biota; Genomics; Oceans; Southern Ocean", "people": "Fabry, Victoria; Hofmann, Gretchen", "repository": "USAP-DC", "science_program": null, "title": "Science of Opportunity: A SGER proposal to support the development of genomic resources for Antarctic pteropods", "url": "https://www.usap-dc.org/view/dataset/600088"}], "date_created": "Sat, 20 Feb 2010 00:00:00 GMT", "description": "This Small Grant for Exploratory Research (SGER) will support the rapid acquisition of DNA sequence for the Antarctic pteropod Limacina helicina, a resource that would allow the development of a cDNA microarray to profile gene expression in this critical marine invertebrate in response to ocean acidification. This request would facilitate the collaboration of the PI (Hofmann), a marine molecular ecologist, with co-PI, Prof. Victoria Fabry, an expert in pteropod calcification biology, and a leader in the ocean acidification research community. Finally, the resources developed here would be shared with the polar research community and all DNA sequence data and protocols would be available via web databases. Notably, the genomic tool developed here would most likely be useful for pteropods from Antarctic and Arctic waters. The broader impacts of this project would be the development of genomic tools for a critical Antarctic marine invertebrate that is threatened by ocean acidification. In addition, these resources would be shared with the polar biology research community.", "east": -150.0, "geometry": "POINT(-175 -73.5)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -70.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Hofmann, Gretchen; Fabry, Victoria", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -77.0, "title": "Science of Opportunity: A SGER proposal to support the development of genomic resources for Antarctic pteropods", "uid": "p0000213", "west": 160.0}, {"awards": "0634682 Kanatous, Shane", "bounds_geometry": "POLYGON((160 -77,160.7 -77,161.4 -77,162.1 -77,162.8 -77,163.5 -77,164.2 -77,164.9 -77,165.6 -77,166.3 -77,167 -77,167 -77.1,167 -77.2,167 -77.3,167 -77.4,167 -77.5,167 -77.6,167 -77.7,167 -77.8,167 -77.9,167 -78,166.3 -78,165.6 -78,164.9 -78,164.2 -78,163.5 -78,162.8 -78,162.1 -78,161.4 -78,160.7 -78,160 -78,160 -77.9,160 -77.8,160 -77.7,160 -77.6,160 -77.5,160 -77.4,160 -77.3,160 -77.2,160 -77.1,160 -77))", "dataset_titles": "The Molecular Signals that Regulate the Ontogeny of Aerobic Capacity, Lipid Metabolism and Elevated Myoglobin Concentrations in the Skeletal Muscles of Weddell Seals", "datasets": [{"dataset_uid": "600063", "doi": "10.15784/600063", "keywords": "Antarctica; Biota; Oceans; Seals; Sea Surface; Southern Ocean", "people": "Kanatous, Shane; Lyons, W. Berry", "repository": "USAP-DC", "science_program": null, "title": "The Molecular Signals that Regulate the Ontogeny of Aerobic Capacity, Lipid Metabolism and Elevated Myoglobin Concentrations in the Skeletal Muscles of Weddell Seals", "url": "https://www.usap-dc.org/view/dataset/600063"}], "date_created": "Fri, 31 Jul 2009 00:00:00 GMT", "description": "During the past three decades, intensive field studies have revealed much about the\u003cbr/\u003ebehavior, physiology, life history, and population dynamics of the Weddell seal (Leptonychotes weddelli) population of McMurdo Sound, Antarctica. These animals are marine predators that are highly adapted for an aquatic life in shore-fast and pack ice habitats. They must locate and capture sparsely distributed under the ice. Most of what is known about their diving behavior is based on studies of adult animals with little known about the development or the genetic controls of diving behavior of young animals. The goal of this project is to examine the temporal development of aerobic capacity, lipid metabolism and oxygen stores in the skeletal muscles of young Weddell seals and to determine which aspects of the cellular environment are important in the regulation of these adaptations during maturation. This project builds on past results to investigate the molecular controls that underlie the development of these adaptations. The first objective is to further characterize the ontogenetic changes in muscle aerobic capacity, lipid metabolism and myoglobin concentration and distribution using enzymatic, immuno-histochemical and myoglobin assays in newly weaned, subadult, and adult seals. The second objective is to determine the molecular controls that regulate these changes in aerobic capacity, fiber type distribution and myoglobin in skeletal muscles during maturation. Through subtractive hybridization and subsequent analysis, differences in mRNA populations in the swimming muscles of the different age classes of Weddell seals will be determined. These techniques will allow for the identification of the proteins and transcription factors that influence the ontogenetic changes in myoglobin concentration, fiber type distribution and aerobic capacity. These results will increase our\u003cbr/\u003eunderstanding of both the ontogeny and molecular mechanisms by which young seals acquire the physiological capabilities to make deep (up to 700 m) and long aerobic dives (ca 20 min). This study will advance knowledge of the molecular regulation for the\u003cbr/\u003eadaptations that enable active skeletal muscle to function under hypoxic conditions; this has a broader application for human medicine especially in regards to cardiac and pulmonary disease. Additional broader impacts include the participation of underrepresented scientists and a continuation of a website in collaboration\u003cbr/\u003ewith the Science Teachers Access to Resources at Southwestern University (STARS Program) which involves weekly updates about research efforts during the field season, weekly questions/answer session involving students and teachers, and updates on research results throughout the year.", "east": 167.0, "geometry": "POINT(163.5 -77.5)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -77.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Kanatous, Shane; Lyons, W. Berry", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -78.0, "title": "The Molecular Signals that Regulate the Ontogeny of Aerobic Capacity, Lipid Metabolism and Elevated Myoglobin Concentrations in the Skeletal Muscles of Weddell Seals", "uid": "p0000536", "west": 160.0}, {"awards": "0739712 Staudigel, Hubertus", "bounds_geometry": "POLYGON((167.04 -77.51,167.067 -77.51,167.094 -77.51,167.121 -77.51,167.148 -77.51,167.175 -77.51,167.202 -77.51,167.229 -77.51,167.256 -77.51,167.283 -77.51,167.31 -77.51,167.31 -77.513,167.31 -77.516,167.31 -77.519,167.31 -77.522,167.31 -77.525,167.31 -77.528,167.31 -77.531,167.31 -77.534,167.31 -77.537,167.31 -77.54,167.283 -77.54,167.256 -77.54,167.229 -77.54,167.202 -77.54,167.175 -77.54,167.148 -77.54,167.121 -77.54,167.094 -77.54,167.067 -77.54,167.04 -77.54,167.04 -77.537,167.04 -77.534,167.04 -77.531,167.04 -77.528,167.04 -77.525,167.04 -77.522,167.04 -77.519,167.04 -77.516,167.04 -77.513,167.04 -77.51))", "dataset_titles": "Metagenome from fumarole sediments sampled from Warren Cave, Antarctica", "datasets": [{"dataset_uid": "000213", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Metagenome from fumarole sediments sampled from Warren Cave, Antarctica", "url": "http://www.ncbi.nlm.nih.gov/bioproject/PRJNA255918"}], "date_created": "Mon, 22 Jun 2009 00:00:00 GMT", "description": "This project studies the microbial processes that alter volcanic glass, which is critical to understanding the earliest life on earth. To understand the environmental controls on these processes, this project uses the extreme environments of the McMurdo region of Antarctica as a natural laboratory. Volcanic glass substrates are placed in hydrothermal systems, lakes, and other areas for two to four years to identify colonizing microbial consortia and the chemical processes of microbe-glass interaction. Recovered experiments are analyzed to explore the role of eukaryotic and prokaryotic organisms, and the relevance of autotrophs during colonization and biofilm formation using microscopic, molecular and culture techniques. \u003cbr/\u003e\u003cbr/\u003eThe broader impacts include graduate and undergraduate student participation in research and K-12 outreach and teacher training.", "east": 167.31, "geometry": "POINT(167.175 -77.525)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -77.51, "nsf_funding_programs": "Antarctic Earth Sciences", "paleo_time": null, "persons": "Staudigel, Hubertus", "platforms": "Not provided", "repo": "NCBI GenBank", "repositories": "NCBI GenBank", "science_programs": null, "south": -77.54, "title": "Collaborative Research: Microbially Mediated Alteration of Volcanic Glass using McMurdo Extreme Environments as Natural Laboratories", "uid": "p0000545", "west": 167.04}, {"awards": "9221598 Mopper, Kenneth", "bounds_geometry": null, "dataset_titles": "Expedition Data", "datasets": [{"dataset_uid": "002282", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/NBP9306"}], "date_created": "Fri, 19 Jun 2009 00:00:00 GMT", "description": "Decreases in stratospheric ozone over the Antarctic result in an increase in the ultraviolet radiation flux in the euphotic zone of the ocean. This increase may lead to cellular damage in aquatic organisms resulting in photo-inhibition and decreased productivity. Cellular damage can occur either intracellularly, or externally at the cell surface from biomolecular reactions with externally-generated reactive transient species. Extracellular damage will depend to a large degree on the photochemistry of the seawater surrounding the cell. To date, little is known about the photochemistry of the unique Antarctic waters. This project integrates a field and laboratory approach to obtain baseline information regarding the marine photochemistry of the euphotic zone in Antarctica waters as related to changes in ultraviolet radiation levels. In situ photochemical production rates and steady state concentrations of a suite of reactive species and dissolved organic matter degradation products as well as downwelling ultraviolet radiation will be measured. Additionally, flux by in situ chemical actinometry, action spectra for photochemical production of various reactive species and dissolved organic matter degradation products, and fluorescence and absorbance properties of dissolved organic matter will be determined. This information will serve as a basis for understanding and predicting the effects of ultraviolet radiation-induced marine photochemical processes on the productivity and ecology in the euphotic zone of the Antarctic Ocean.", "east": null, "geometry": null, "instruments": "EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS", "is_usap_dc": false, "keywords": "R/V NBP", "locations": null, "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Mopper, Kenneth; Neale, Patrick", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repo": "R2R", "repositories": "R2R", "science_programs": null, "south": null, "title": "Photochemistry of Antarctic Waters in Repsonse to Changing Ultraviolet Radiation Fluxes", "uid": "p0000649", "west": null}, {"awards": "0230276 Ward, Bess", "bounds_geometry": "POLYGON((162 -77.2,162.16 -77.2,162.32 -77.2,162.48 -77.2,162.64 -77.2,162.8 -77.2,162.96 -77.2,163.12 -77.2,163.28 -77.2,163.44 -77.2,163.6 -77.2,163.6 -77.26,163.6 -77.32,163.6 -77.38,163.6 -77.44,163.6 -77.5,163.6 -77.56,163.6 -77.62,163.6 -77.68,163.6 -77.74,163.6 -77.8,163.44 -77.8,163.28 -77.8,163.12 -77.8,162.96 -77.8,162.8 -77.8,162.64 -77.8,162.48 -77.8,162.32 -77.8,162.16 -77.8,162 -77.8,162 -77.74,162 -77.68,162 -77.62,162 -77.56,162 -77.5,162 -77.44,162 -77.38,162 -77.32,162 -77.26,162 -77.2))", "dataset_titles": "What Limits Denitrification and Bacterial Growth in Lake Bonney, Taylor Valley, Antarctica?", "datasets": [{"dataset_uid": "600033", "doi": "10.15784/600033", "keywords": "Antarctica; Biota; CTD Data; Dry Valleys; Lake Bonney; Lake Vanda; Microbiology; Taylor Valley", "people": "Ward, Bess", "repository": "USAP-DC", "science_program": null, "title": "What Limits Denitrification and Bacterial Growth in Lake Bonney, Taylor Valley, Antarctica?", "url": "https://www.usap-dc.org/view/dataset/600033"}], "date_created": "Sun, 18 Jan 2009 00:00:00 GMT", "description": "Denitrification is the main process by which fixed nitrogen is lost from ecosystems and the regulation of this process may directly affect primary production and carbon cycling over short and long time scales. Previous investigations of the role of bioactive metals in regulating denitrification in bacteria from permanently ice-covered Lake Bonney in the Taylor Valley of East Antarctica indicated that denitrifying bacteria can be negatively affected by metals such as copper, iron, cadmium, lead, chromium, nickel, silver and zinc; and that there is a distinct difference in denitrifying activity between the east and west lobes of the lake. Low iron concentrations were found to exacerbate the potential toxicity of the other metals, while silver has the potential to specifically inhibit denitrification because of its ability to interfere with copper binding in redox proteins, such as nitrite reductase and nitrous oxide reductase. High silver concentrations might prevent the functioning of nitrous oxide reductase in the same way that simple copper limitation does, thereby causing the buildup of nitrous oxide and resulting in a nonfunctional nitrogen cycle. Other factors, such as oxygen concentration, are likely also to affect bacterial activity in Lake Bonney. This project will investigate silver toxicity, general metal toxicity and oxygen concentration to determine their effect on denitrification in the lake by using a suite of \"sentinel\" strains of denitrifying bacteria (isolated from the lake) incubated in Lake Bonney water and subjected to various treatments. The physiological responses of these strains to changes in metal and oxygen concentration will be quantified by flow cytometric detection of single cell molecular probes whose sensitivity and interpretation have been optimized for the sentinel strains. Understanding the relationships between metals and denitrification is expected to enhance our understanding of not only Lake Bonney\u0027s unusual nitrogen cycle, but more generally, of the potential role of metals in the regulation of microbial nitrogen transformations.\u003cbr/\u003e\u003cbr/\u003eThe broader impacts of this work include not only a better understanding of regional biogeochemistry and global perspectives on these processes; but also the training of graduate students and a substantial outreach effort for school children.", "east": 163.6, "geometry": "POINT(162.8 -77.5)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD", "is_usap_dc": true, "keywords": "FIELD SURVEYS", "locations": null, "north": -77.2, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Ward, Bess", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -77.8, "title": "Collaborative Research: What Limits Denitrification and Bacterial Growth in Lake Bonney, Taylor Valley, Antarctica?", "uid": "p0000223", "west": 162.0}, {"awards": "0636706 Sivjee, Gulamabas", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "NCAR Coupling, Energetics and Dynamics of Atmospheric Regions (CEDAR) Data System ID# 5700 (full data link not provided)", "datasets": [{"dataset_uid": "000137", "doi": "", "keywords": null, "people": null, "repository": "NCAR", "science_program": null, "title": "NCAR Coupling, Energetics and Dynamics of Atmospheric Regions (CEDAR) Data System ID# 5700 (full data link not provided)", "url": "http://cedarweb.hao.ucar.edu/"}], "date_created": "Thu, 23 Oct 2008 00:00:00 GMT", "description": "This project will provide for the continued operation and data analysis of an electro-optical remote sensing facility at South Pole Station. The facility will be used to examine 1) the source(s) and propagation of patches of enhanced plasma density in the F-region of the Antarctic ionosphere, 2) changes in the Antarctic E-region O/N2 ratio in the center of the night-sector of the auroral oval and compare the ratios with those found in the sun-aligned auroral arcs in the Polar Cap region, 3) Antarctic middle atmosphere disturbances generated by Stratospheric Warming Events (SWE), 4) quantitative characterization of the effects of solar variability on the temperature of the upper mesosphere region, 5) Antarctic thermospheric response to Solar Magnetic Cloud/Coronal Mass Ejection (SMC/CME) events, and 6) the effects of Joule heating on the thermodynamics of the Antarctic F-region. Data for all these studies will come from two sets of remote-sensing facilities at SPS: 1) Auroral emissions brightness measurements from the sun-synchronous Meridian Scanning Photon Counting Multichannel photometer; 2) Airglow and Auroral emission spectra recorded continuously during Austral winter at SPS with the high throughput, high resolution Infrared Michelson Interferometer as well as Visible - Near Infrared CCD spectrographs. \u003cbr/\u003e\u003cbr/\u003eMeridional variations in the brightness of F-region\u0027s auroral emissions provide the necessary data for investigations of the dynamics and IMF control, as well as the excitation mechanism(s), of the F-region patches. The brightness of auroral emissions from O and N relative to those from molecular species (O2 and N2) can be analyzed to assess, quantitatively, changes in the thermospheric composition. These data (from continuous (24 hours a day) measurements during the totally dark six months of each Austral winter at SPS) will be used to investigate the effects of solar-terrestrial disturbances on Antarctic thermospheric composition and thermodynamics, including response of the mesopause to solar cycle variations. Changes in airglow temperature (derived from OH and O2 bands), from different mesosphere/lower-thermosphere (MLT) heights, permit studies of the dynamical effects of Planetary, Tidal and Gravity waves propagating in the MLT regions as well as non-linear interactions among these waves. Coupling of different atmospheric regions over SPS, through enhanced gravity wave activities during SWE that lead to a precursor as Mesospheric cooling, will be investigated through the observed changes in MLT kinetic air temperature and density. \u003cbr/\u003e\u003cbr/\u003eThe project will enhance the infrastructure for research and education at Embry-Riddle Aeronautical University, bringing together the PI/Co-I and students from Departments of Physical Sciences and Aerospace Engineering. Graduate and undergraduate students will participate in modern research and software development.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -60.0, "nsf_funding_programs": "Antarctic Astrophysics and Geospace Sciences", "paleo_time": null, "persons": "Gulamabas, Sivjee; Azeem, Syed", "platforms": "Not provided", "repo": "NCAR", "repositories": "NCAR", "science_programs": null, "south": -90.0, "title": "Observations of Upper Atmospheric Energetics, Dynamics, and Long-Term Variations over the South Pole Station", "uid": "p0000292", "west": -180.0}, {"awards": "0338267 Gooseff, Michael", "bounds_geometry": "POLYGON((161.6 -77.4,161.773 -77.4,161.946 -77.4,162.119 -77.4,162.292 -77.4,162.465 -77.4,162.638 -77.4,162.811 -77.4,162.984 -77.4,163.157 -77.4,163.33 -77.4,163.33 -77.435,163.33 -77.47,163.33 -77.505,163.33 -77.54,163.33 -77.575,163.33 -77.61,163.33 -77.645,163.33 -77.68,163.33 -77.715,163.33 -77.75,163.157 -77.75,162.984 -77.75,162.811 -77.75,162.638 -77.75,162.465 -77.75,162.292 -77.75,162.119 -77.75,161.946 -77.75,161.773 -77.75,161.6 -77.75,161.6 -77.715,161.6 -77.68,161.6 -77.645,161.6 -77.61,161.6 -77.575,161.6 -77.54,161.6 -77.505,161.6 -77.47,161.6 -77.435,161.6 -77.4))", "dataset_titles": "Antarctic Hydrologic Margin Microbiology and Biogeochemistry - data; Hydrologic Margins Research Project, 2004-2008, McMurdo Dry Valleys", "datasets": [{"dataset_uid": "600016", "doi": "", "keywords": null, "people": "Gooseff, Michael N.", "repository": "USAP-DC", "science_program": null, "title": "Hydrologic Margins Research Project, 2004-2008, McMurdo Dry Valleys", "url": "https://www.usap-dc.org/view/dataset/600016"}, {"dataset_uid": "000238", "doi": "", "keywords": null, "people": null, "repository": "PI website", "science_program": null, "title": "Antarctic Hydrologic Margin Microbiology and Biogeochemistry - data", "url": "http://water.engr.psu.edu/gooseff/web_antarctica/data.html"}], "date_created": "Thu, 11 Sep 2008 00:00:00 GMT", "description": "Aquatic-terrestrial transition zones are crucial environments in understanding the biogeochemistry of landscapes. In temperate watersheds, these areas are generally dominated by riparian zones, which have been identified as regions of special interest for biogeochemistry because of the increased microbial activity in these locations, and because of the importance of these hydrological margins in facilitating and buffering hydrologic and biogeochemical exchanges between terrestrial and aquatic ecosystems. In the Antarctic Dry Valleys, terrestrial-aquatic transition zones are intriguing landscape features because of the vast importance of water in this polar desert, and because the material and energy budgets of dry valley ecosystems are linked by hydrology. Hydrological margins in aquatic-terrestrial transition zones will be studied in the Dry Valleys of Antarctica to answer two overarching questions: (1) what are the major controls over hydrologic and biogeochemical exchange across aquaticterrestrial transition zones and (2) to what extent do trends in nutrient cycling (e.g. nitrogen cycling) across these transition zones reflect differences in microbial communities or function vs. differences in the physical and chemical environment (e.g., redox potential)? The hydrologic gradients that define these interfaces provide the opportunity to assess the relative influence of physical conditions and microbial biodiversity and functioning upon biogeochemical cycling. Coordinated hydrologic, biogeochemical, and molecular microbial studies will be executed within hydrologic margins with the following research objectives: to determine the role of sediment characteristics, permafrost and active layer dynamics, and topography on sub-surface water content and distribution in hydrologic margins, to determine the extent to which transformations of nitrogen in hydrological margins are influenced by physical conditions (i.e., moisture, redox potential and pH) or by the presence of specific microbial communities (e.g., denitrifiers), and to characterize the microbial community structure and function of saturated zones.\u003cbr/\u003e\u003cbr/\u003eThis proposed research will provide an improved understanding of the interaction of liquid water, soils, microbial communities, and biogeochemistry within the important hydrologic margin landscape units of the dry valleys. Dry valleys streams and lakes are unique because there is no influence of higher vegetation on the movement of water and may therefore provide a model system for understanding physical and hydrological influences on microbial ecology and biogeochemistry. Hence the findings will contribute to Antarctic science as well as the broader study of riparian zones and hydrologic margins worldwide. Graduate students and undergraduate students will be involved with fieldwork and research projects. Information will be disseminated through a project web site, and outreach activities will include science education in local elementary, middle and high schools near the three universities involved.", "east": 163.33, "geometry": "POINT(162.465 -77.575)", "instruments": null, "is_usap_dc": false, "keywords": "Not provided", "locations": null, "north": -77.4, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Gooseff, Michael N.; Barrett, John; Takacs-Vesbach, Cristina", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "PI website; USAP-DC", "science_programs": null, "south": -77.75, "title": "Collaborative Research: Hydrologic Controls over Biogeochemistry and Microbial Community Structure and Function across Terrestrial/Aquatic Interfaces in a Polar Desert", "uid": "p0000340", "west": 161.6}, {"awards": "0536870 Rogers, Scott", "bounds_geometry": "POINT(-106.8 -72.4667)", "dataset_titles": "Comprehensive Biological Study of Vostok Accretion Ice", "datasets": [{"dataset_uid": "600052", "doi": "10.15784/600052", "keywords": "Antarctica; Biota; Cryosphere; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Hydrothermal Vent; Lake Vostok; Microbes; Subglacial Lake", "people": "Rogers, Scott O.", "repository": "USAP-DC", "science_program": null, "title": "Comprehensive Biological Study of Vostok Accretion Ice", "url": "https://www.usap-dc.org/view/dataset/600052"}], "date_created": "Tue, 02 Sep 2008 00:00:00 GMT", "description": "The large subglacial Lake Vostok in Antarctica is unique ecological site with a novel microbial biota. The temperatures, pressures and lack of light all select for organisms that may not exist anywhere else on Earth. The accretion ice (lake water frozen to the bottom of the lower surface of the glacier) has preserved microbial samples from each region of Lake Vostok as the glacier passes over and into the lake. Thus, without contaminating the lake with microorganisms from the surface, microbes originating from the lake can be collected, transported to the laboratory and studied. Two of the deepest ice cores sections in this project are part of the international allocation. The will be shared between four researchers (Sergey Bulat from Russia, Jean-Robert Petit and Daniel Prieur from France, Scott Rogers from USA). The United States team will study, isolate, and characterize bacteria, fungi, and viruses that have been sampled from the lake through the process of ice accretion to the lower surface of 3500+m thick glacier overriding the lake. The project will involve a suite of methods, including molecular, morphological, and cultural. This includes observation and description by fluorescence, light, and electron microscopy, isolation on thirteen separate cultural media, polymerase chain reaction amplification, DNA sequencing, and phylogenetic analyses. Eleven accretion ice core sections, as well as two glacial ice core sections. As well as two glacial ice core sections will be studied. The accretion ice core sections, as well as two glacial ice core sections will be studied. The accretion ice core sections represent all of the major regions of the lake that have been sampled by the accretion process in the vicinity of the Vostok 5G ice core. The broader impacts of the work relate to the impact the results will have on the filed. These long=isolated lakes, deep below the Antarctic ice sheet may contain novel uniquely adapted organisms. Glacial ice contains an enormous diversity of entrapped microbes, some of which may be metabolically active in the ice. The microbes from Lake Vostok are of special interest, since they are adapted to cold, dark, and high pressure. Thus, their enzyme systems and biochemical pathways may be significantly different from those in the microbes that are the subject of current studies. As such, these organisms may form compounds that may have useful applications. Also, study of the accretion ice, and eventually the water, from Lake Vostok will provide a basis for the study of other subglacial lakes. Additionally, study of the microbes in the accretion ice will be useful to those planning to study analogous systems on ice-covered planets and moons.", "east": -106.8, "geometry": "POINT(-106.8 -72.4667)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -72.4667, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Rogers, Scott O.", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -72.4667, "title": "Comprehensive Biological Study of Vostok Accretion Ice", "uid": "p0000566", "west": -106.8}, {"awards": "0338087 Scheltema, Rudolf; 0338218 Halanych, Kenneth", "bounds_geometry": "POLYGON((-70 -55,-68 -55,-66 -55,-64 -55,-62 -55,-60 -55,-58 -55,-56 -55,-54 -55,-52 -55,-50 -55,-50 -56,-50 -57,-50 -58,-50 -59,-50 -60,-50 -61,-50 -62,-50 -63,-50 -64,-50 -65,-52 -65,-54 -65,-56 -65,-58 -65,-60 -65,-62 -65,-64 -65,-66 -65,-68 -65,-70 -65,-70 -64,-70 -63,-70 -62,-70 -61,-70 -60,-70 -59,-70 -58,-70 -57,-70 -56,-70 -55))", "dataset_titles": "Expedition Data; Expedition data of LMG0414; Expedition data of LMG0605; Relevance of Planktonic Larval Dispersal to Endemism and Biogeography of Antarctic Benthic Invertebrates", "datasets": [{"dataset_uid": "600035", "doi": "10.15784/600035", "keywords": "Antarctica; Biota; Oceans; R/v Laurence M. Gould; Sample/collection Description; Sample/Collection Description; Southern Ocean", "people": "Scheltema, Rudolf", "repository": "USAP-DC", "science_program": null, "title": "Relevance of Planktonic Larval Dispersal to Endemism and Biogeography of Antarctic Benthic Invertebrates", "url": "https://www.usap-dc.org/view/dataset/600035"}, {"dataset_uid": "002711", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0414", "url": "https://www.rvdata.us/search/cruise/LMG0414"}, {"dataset_uid": "002682", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0605", "url": "https://www.rvdata.us/search/cruise/LMG0605"}, {"dataset_uid": "001565", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0414"}], "date_created": "Wed, 18 Jun 2008 00:00:00 GMT", "description": "Because of extreme isolation of the Antarctic continent since the Early Oligocene, one expects a unique invertebrate benthic fauna with a high degree of endemism. Yet some invertebrate taxa that constitute important ecological components of sedimentary benthic communities include more than 40 percent non-endemic species (e.g., benthic polychaetes). To account for non-endemic species, intermittent genetic exchange must occur between Antarctic and other (e.g. South American) populations. The most likely mechanism for such gene flow, at least for in-faunal and mobile macrobenthos, is dispersal of planktonic larvae across the sub- Antarctic and Antarctic polar fronts. To test for larval dispersal as a mechanism of maintaining genetic continuity across polar fronts, the scientists propose to (1) take plankton samples along transects across Drake passage during both the austral summer and winter seasons while concurrently collecting the appropriate hydrographic data. Such data will help elucidate the hydrographic mechanisms that allow dispersal across Drake Passage. Using a molecular phylogenetic approach, they will (2) compare seemingly identical adult forms from Antarctic and South America continents to identify genetic breaks, historical gene flow, and control for the presence of cryptic species. (3) Similar molecular tools will be used to relate planktonic larvae to their adult forms. Through this procedure, they propose to link the larval forms respectively to their Antarctic or South America origins. The proposed work builds on previous research that provides the basis for this effort to develop a synthetic understanding of historical gene flow and present day dispersal mechanism in South American/Drake Passage/Antarctic Peninsular region. Furthermore, this work represents one of the first attempts to examine recent gene flow in Antarctic benthic invertebrates. Graduate students and a postdoctoral fellow will be trained during this research.", "east": -50.0, "geometry": "POINT(-60 -60)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; IN SITU/LABORATORY INSTRUMENTS \u003e PRESSURE/HEIGHT METERS \u003e PRESSURE SENSORS; IN SITU/LABORATORY INSTRUMENTS \u003e PHOTON/OPTICAL DETECTORS \u003e TURBIDITY METERS; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ADCP", "is_usap_dc": true, "keywords": "EU735823-EU735850; R/V LMG; FIELD SURVEYS; Genbank Ef565745-Ef565820; Not provided", "locations": null, "north": -55.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Scheltema, Rudolf; Halanych, Kenneth", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS; Not provided; WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "USAP-DC", "repositories": "R2R; USAP-DC", "science_programs": null, "south": -65.0, "title": "Collaborative Research: Relevance of Planktonic Larval Dispersal to Endemism and Biogeography of Antarctic Benthic Invertebrates", "uid": "p0000189", "west": -70.0}, {"awards": "0337656 Lee, Richard", "bounds_geometry": "POLYGON((-64.1 -64.75,-64.085 -64.75,-64.07 -64.75,-64.055 -64.75,-64.04 -64.75,-64.025 -64.75,-64.01 -64.75,-63.995 -64.75,-63.98 -64.75,-63.965 -64.75,-63.95 -64.75,-63.95 -64.757,-63.95 -64.764,-63.95 -64.771,-63.95 -64.778,-63.95 -64.785,-63.95 -64.792,-63.95 -64.799,-63.95 -64.806,-63.95 -64.813,-63.95 -64.82,-63.965 -64.82,-63.98 -64.82,-63.995 -64.82,-64.01 -64.82,-64.025 -64.82,-64.04 -64.82,-64.055 -64.82,-64.07 -64.82,-64.085 -64.82,-64.1 -64.82,-64.1 -64.813,-64.1 -64.806,-64.1 -64.799,-64.1 -64.792,-64.1 -64.785,-64.1 -64.778,-64.1 -64.771,-64.1 -64.764,-64.1 -64.757,-64.1 -64.75))", "dataset_titles": null, "datasets": null, "date_created": "Fri, 06 Jun 2008 00:00:00 GMT", "description": "Polar terrestrial environments are often described as deserts, where water availability is recognized as one of the most important limits on the distribution of terrestrial organisms. In addition, prolonged low winter temperatures threaten survival, and summer temperatures challenge organisms with extensive diel variations and rapid transitions from freezing to desiccating conditions. Global warming has further impacted the extreme thermal and hydric conditions experienced by Antarctic terrestrial plant and arthropod communities, especially as a result of glacial retreat along the Antarctic Peninsula. This research will focus on thermal and hydric adaptations in the terrestrial midge, Belgica antarctica, the largest and most southerly holometabolous insect living in this challenging and changing environment. \u003cbr/\u003eOverwintering midge larvae encased in the frozen substrate must endure desert-like conditions for more than 300 days since free water is biologically unavailable as ice. During the summer, larvae may be immersed in melt water or outwash from penguin colonies and seal wallows, in addition to saltwater splash. Alternatively, the larvae may be subjected to extended periods of desiccation as their microhabitats dry out. Due to their small size, relative immobility and the patchiness of suitable microhabitats, larvae may thus be subjected to stresses that include desiccation, hypo- or hyperosmotic conditions, high salinity exposure, and anoxia for extended periods. Research efforts will focus in three areas relevant to the stress tolerance mechanisms operating in these midges:(1) obtaining a detailed characterization of microclimatic conditions experienced by B. antarctica, especially those related to thermal and hydric diversity, both seasonally and among microhabitat types in the vicinity of Palmer Station, Antarctica; (2) examining the effects of extreme fluctuations in water availability and effects on physiological and molecular responses - to determine if midge larvae utilize the mechanism of cryoprotective dehydration for winter survival, and if genes encoding heat shock proteins and other genes are upregulated in larval responses to dehydration and rehydration; (3) investigating the dietary transmission of cryoprotectants from plant to insect host, which will test the hypothesis that midge larvae acquire increased resistance to desiccation and temperature stress by acquiring cryoprotectants from their host plants. \u003cbr/\u003eThis project will provide outreach to both elementary and secondary educators and their students. The team will include a teacher who will benefit professionally by full participation in the research, and will also assist in providing outreach to other teachers and their students. From Palmer Station, the field team will communicate daily research progress by e-mail supplemented with digital pictures with teachers and their elementary students to stimulate interest in an Antarctic biology and scientific research. These efforts will be supplemented with presentations at local schools and national teacher meetings, and by publishing hands-on, inquiry-based articles related to cryobiology and polar biology in education journals. Furthermore, the principal investigators will maintain major commitments to training graduate students and postdoctoral scholars, as well as undergraduate students by providing extended research experience that includes publication of scientific papers and presentations at national meetings.", "east": -63.95, "geometry": "POINT(-64.025 -64.785)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -64.75, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Denlinger, David; Lee, Richard", "platforms": "Not provided", "repositories": null, "science_programs": null, "south": -64.82, "title": "Physiological and Molecular Mechanisms of Stress Tolerance in a Polar Insect", "uid": "p0000742", "west": -64.1}, {"awards": "0230452 Severinghaus, Jeffrey", "bounds_geometry": "POINT(124.5 -80.78)", "dataset_titles": "Antarctic megadunes", "datasets": [{"dataset_uid": "000191", "doi": "", "keywords": null, "people": null, "repository": "NSIDC", "science_program": null, "title": "Antarctic megadunes", "url": "http://nsidc.org/antarctica/megadunes/"}], "date_created": "Wed, 27 Sep 2006 00:00:00 GMT", "description": "This award supports a study of the chemical composition of air in the snow layer (firn) in a region of \"megadunes\" near Vostok station, Antarctica. It will test the hypothesis that a deep \"convective zone\" of vigorous wind-driven mixing can prevent gas fractionation in the upper one-third of the polar firn layer. In the megadunes, ultralow snow accumulation rates lead to structural changes (large grains, pipes, and cracks) that make the permeability of firn to air movement orders of magnitude higher than normal. The unknown thickness of the convective zone has hampered the interpretation of ice core 15N/14N and 40Ar/36Ar ratios as indicators of past firn thickness, which is a key constraint on the climatically important variables of temperature, accumulation rate, and gas age-ice age difference. Studying this \"extreme end-member\" example will better define the role of the convective zone in gas reconstructions. This study will pump air from a profile of ~20 depths in the firn, to definitively test for the presence of a convective zone based on the fit of observed 15 N/14N and 40Ar/36Ar to a molecular- and eddy-diffusion model. Permeability measurements on the core and 2-D air flow modeling (in collaboration with M. Albert) will permit a more physically realistic interpretation of the isotope data and will relate mixing vigor to air velocities. A new proxy indicator of convective zone thickness will be tested on firn and ice core bubble air, based on the principle that isotopes of slow-diffusing heavy noble gases (Kr, Xe) should be more affected by convection than isotopes of fast-diffusing N2 . These tools will be applied to a test of the hypothesis that the megadunes and a deep convective zone existed at the Vostok site during glacial periods, which would explain the anomalously low 15N/14N and 40Ar/36Ar in the Vostok ice core glacial periods. The broader impacts of this work include 1) clarification of phase relationships of greenhouse gases and temperature in ice core records, with implications for understanding of past and future climates, 2) education of one graduate student, and 3) building of collaborative relationships with five investigators.", "east": 124.5, "geometry": "POINT(124.5 -80.78)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e CORERS \u003e CORING DEVICES; IN SITU/LABORATORY INSTRUMENTS \u003e SAMPLERS \u003e BOTTLES/FLASKS/JARS \u003e FLASKS", "is_usap_dc": false, "keywords": "Antarctica; Methane; Carbon-14; Permeability; CO2; Firn Core; FIELD SURVEYS; Deuterium Excess; GROUND-BASED OBSERVATIONS; LABORATORY; Isotope; Ice Core Density; Firn Air; Megadunes; Ice Core; Not provided; FIELD INVESTIGATION", "locations": "Antarctica", "north": -80.78, "nsf_funding_programs": "Antarctic Glaciology", "paleo_time": null, "persons": "Bauer, Rob; Albert, Mary R.; Severinghaus, Jeffrey P.", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION; LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS; LAND-BASED PLATFORMS \u003e PERMANENT LAND SITES \u003e GROUND-BASED OBSERVATIONS; Not provided; OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repo": "NSIDC", "repositories": "NSIDC", "science_programs": null, "south": -80.78, "title": "How Thick Is the Convective Zone: A Study of Firn Air in the Megadunes Near Vostok, Antarctica", "uid": "p0000097", "west": 124.5}]

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