IEDA
Project Information
Analysis of Voltage-gated Ion Channels in Antarctic Fish
Start Date:
2015-04-01
End Date:
2018-03-31
Description/Abstract
We studied the molecular evolution and physiology of two types of ion channels: voltage gated potassium channels and transient receptor potential (TRP) channels. We also studied the molecular evolution and expression of water-passing channels, the aquaporins, to determine if these show signs of evolutionary change in notothenioids. We noted apparent amino acid substitutions at a number of sites in a muscle-expressing potassium channel (Kv1.3). We were surprised to find that although the AAs at these sites appeared highly conserved in teleosts and even in tetrapods, reverting them singly, in pairs, or all together back to the ancestral condition had no effect on the biophysical properties of the channels that we measured (voltage-sensitivity; rate of activation) at room temperature as well as over a range of temperatures down to 4oC. The results for the TRP channels and aquaporins can be accessed in their publications. York and Zakon (2022) in Genome Biology and Evolution, and two forthcoming papers.
Personnel
Person Role
Zakon, Harold Investigator and contact
York, Julia Researcher
Funding
Antarctic Organisms and Ecosystems Award # 1443637
AMD - DIF Record(s)
Data Management Plan
None in the Database
Product Level:
1 (processed data)
Publications
  1. Julia M York, Harold H Zakon, Evolution of Transient Receptor Potential (TRP) Ion Channels in Antarctic Fishes (Cryonotothenioidea) and Identification of Putative Thermosensors, Genome Biology and Evolution, Volume 14, Issue 2, February 2022, evac009 (doi:10.1093/gbe/evac009)
Keywords
Platforms and Instruments

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