IEDA
Project Information
RUI: Characterizing Protein Homeostasis and the Regulatory Mechanisms Controlling Molecular Chaperone Expression in the Highly Stenothermal Notothenioid Fish, Trematomus Bernacchii
Short Title:
Protein homeostasis
Start Date:
2016-08-01
End Date:
2020-07-31
Project Location(s)
McMurdo Sound
Description/Abstract
The project will integrate analyses of fish physiology, protein production and genetics to determine if regulation of molecular chaperones (a class of proteins that facilitate the proper folding of proteins in a cell) has been permanently lost in a key fish species (Trematomus bernacchii) inhabiting the Southern Ocean. To do so, efforts will be undertaken to analyze chaperones in these fishes and how elevated temperatures impact protein turnover and protein damage. These studies should more definitively determine if the interruption of chaperone function is environmentally controlled (which could suggest these fish could benefit in some form by increasing sea surface temperatures) or if there is complete loss of chaperone function due to a change in its structure through evolutionary processes (which would suggest these fish are less likely to be able to adapt to warming). In addition to filling key gaps in our knowledge about the diversity and evolution of fishes in the southern ocean and the potential impacts changing temperatures might have on fish populations, the project will support the training of undergraduate and graduate students at an RUI institution. Specifically, activities and content directly related to this project's aims will be incorporated into the undergraduate curriculum at Sonoma State University in an effort to increase undergraduate participation in research, especially with respect to underrepresented groups.

The project has specific aims to perform a comparative analysis of nucleotide divergence resulting in non-synonymous amino acid changes in the trans-regulatory elements, namely members of the heat shock factor (HSF) family of transcription factors, in T. bernacchii and N. angustata. The project will also utilize metabolic labeling of newly synthesized proteins from isolated hepatocytes to monitor protein turnover rates in fish acclimated to both -1.5 and +4 °C for an extended period. Changes in chaperoning capacity and levels of damaged proteins will be quantified in multiple tissues to gain a better understanding of the cellular requirements for maintaining protein homeostasis under long-term acclimations to +4 °C. In combination, the work will help answer questions regarding divergence in these fishes as well a fundamental information regarding protein structure and function that may also have bio-medical implications.
Personnel
Person Role
Place, Sean Investigator and contact
Funding
Antarctic Organisms and Ecosystems Award # 1543419
AMD - DIF Record(s)
Data Management Plan
None in the Database
Product Level:
0 (raw data)
Datasets
Repository Title (link) Format(s) Status
NCBI GenBank miRNA Characterization in Antarctic fish None exists
NCBI GenBank Hspa4 MK948003 None no link
NCBI GenBank Hspa4 MK948003 None no link
NCBI GenBank Hsp90ab MK948004 None no link
NCBI GenBank Hsc71 MK948005 None no link
NCBI GenBank Hsp40 MK948006 None no link
NCBI GenBank Hspa6 MN367221 None no link
NCBI GenBank Hspa12a MN367222 None no link
NCBI GenBank Hspa13 MN367223 None no link
NCBI GenBank Hsc71 MN045883 None no link
NCBI GenBank Hsp40 MN045884 None no link
NCBI GenBank Hsp90aB MN045885 None no link
NCBI GenBank Hspa4 MN045886 None no link
NCBI GenBank Hspa12a MN367224 None no link
NCBI GenBank Hspa13 MN367225 None no link
NCBI GenBank HspA6 MN367226 None no link
NCBI GenBank Hsp90b MN367227 None no link
Publications
  1. Vasadia, D.J., Zippay, M.L., Place, S.P. (2019). Characterization of thermally sensitive miRNAs reveals a central role of the FoxO signaling pathway in regulating the cellular stress response of an extreme stenotherm, Trematomus bernacchii. Marine Genomics. (doi:10.1016/j.margen.2019.100698)
  2. Tolomeo, A.M., Carraro, A, Bakiu, R, Toppo, S., Garofalo, F., Pellegrino, D., Gerdol, M., Ferro, D., Place,S.P., Santovito, G. (2019). Molecular characterization of novel mitochondrial peroxiredoxins from the Antarctic emerald rockcod and their gene expression in response to environmental warming. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 225: (doi:10.1016/j.cbpc.2019.108580)
  3. Enzor, L.A., Hunter, E.M. and S.P. Place (2017). The effects of elevated temperature and ocean acidification on the metabolic pathways of notothenioid fish. Conserv Physiol. 5 (1): cox019. (doi:10.1093/conphys/cox019)
  4. Tercero, A. D., & Place, S. P. (2020). Characterizing Gene Copy Number of Heat Shock Protein Gene Families in the Emerald Rockcod, Trematomus bernacchii. Genes, 11(8), 867. (doi:10.3390/genes11080867)
Platforms and Instruments

This project has been viewed 74 times since May 2019 (based on unique date-IP combinations)