USAP-DC

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{"@context": "https://schema.org/", "@type": "Dataset", "@id": "doi:10.15784/601405", "additionalType": ["geolink:Dataset", "vivo:Dataset"], "name": "Cardiac metabolism in Antarctic fishes in response to an acute increase in temperature", "description": "Studies in temperate fishes provide evidence that cardiac mitochondrial function and the capacity to fuel cardiac work contribute to thermal tolerance. Here, we tested the hypothesis that decreased cardiac aerobic metabolic capacity contributes to the lower thermal tolerance of the haemoglobinless Antarctic icefish, Chaenocephalus aceratus, compared with that of the red-blooded Antarctic species, Notothenia coriiceps. Maximal activities of citrate synthase (CS) and lactate dehydrogenase (LDH), respiration rates of isolated mitochondria, adenylate levels and changes in mitochondrial protein expression were quantified from hearts of animals held at ambient temperature or exposed to their critical thermal maximum (CTmax). Compared with C. aceratus, activity of CS, ATP concentration and energy charge were higher in hearts of N. coriiceps at ambient temperature and CTmax While state 3 mitochondrial respiration rates were not impaired by exposure to CTmax in either species, state 4 rates, indicative of proton leakage, increased following exposure to CTmax in C. aceratus but not N. coriiceps The interactive effect of temperature and species resulted in an increase in antioxidants and aerobic metabolic enzymes in N. coriiceps but not in C. aceratus Together, our results support the hypothesis that the lower aerobic metabolic capacity of C. aceratus hearts contributes to its low thermal tolerance. ", "citation": "O'Brien, K. (2020) \"Cardiac metabolism in Antarctic fishes in response to an acute increase in temperature\" U.S. Antarctic Program (USAP) Data Center. doi: https://doi.org/10.15784/601405.", "datePublished": "2020-12-18", "keywords": ["Antarctica", "Antarctic Peninsula"], "creator": [{"@type": "Person", "additionalType": "geolink:Person", "name": "O'Brien, Kristin", "email": "kmobrien@alaska.edu", "affiliation": {"@type": "Organization", "name": "University of Alaska"}}], "distribution": [{"@type": "DataDownload", "additionalType": "http://www.w3.org/ns/dcat#DataCatalog", "encodingFormat": "text/xml", "name": "ISO Metadata Document", "url": "https://www.usap-dc.org/metadata/isoxml/601405iso.xml", "contentUrl": "/dataset/filename"}, {"@type": "DataDownload", "@id": "http://dx.doi.org/10.15784/601405", "additionalType": "dcat:distribution", "url": "http://dx.doi.org/10.15784/601405", "name": "landing page", "description": "Link to a web page related to the resource.. Service Protocol: Link to a web page related to the resource.. Link Function: information", "contentUrl": "/dataset/filename", "encodingFormat": "text/html"}, {"@type": "DataDownload", "@id": "https://www.usap-dc.org/view/dataset/601405", "additionalType": "dcat:distribution", "url": "https://www.usap-dc.org/view/dataset/601405", "name": "landing page", "description": "Link to a web page related to the resource.. Service Protocol: Link to a web page related to the resource.. Link Function: information", "contentUrl": "/dataset/filename", "encodingFormat": "text/html"}], "identifier": {"@type": "PropertyValue", "propertyID": "https://registry.identifiers.org/registry/doi", "value": "doi:10.15784/601405", "url": "https://doi.org/10.15784/601405"}, "contributor": [{"@type": "Role", "roleName": "credit", "description": "funderName:NSF:GEO:PLR:Antarctic Organisms and Ecosystems awardNumber:1341663 awardTitle:Collaborative Research: The Physiological and Biochemical Underpinnings of Thermal Tolerance in Antarctic Notothenioid Fishes"}], "license": [{"@type": "CreativeWork", "URL": "https://creativecommons.org/licenses/by/4.0/", "name": "MD_Constraints", "description": "useLimitation: Creative Commons Attribution Only v4.0 Generic [CC BY 4.0]."}, {"@type": "CreativeWork", "name": "MD_LegalConstraints", "description": "accessConstraints: license. otherConstraints: Creative Commons Attribution Only v4.0 Generic [CC BY 4.0]."}, {"@type": "CreativeWork", "name": "MD_SecurityConstraints", "description": "classification: "}], "publisher": {"@type": "Organization", "name": "U.S. Antarctic Program (USAP) Data Center"}, "spatialCoverage": []}

Cardiac metabolism in Antarctic fishes in response to an acute increase in temperature Data DOI: https://doi.org/10.15784/601405 Cite as O'Brien, K. (2020) "Cardiac metabolism in Antarctic fishes in response to an acute increase in temperature" U.S. Antarctic Program (USAP) Data Center. doi: https://doi.org/10.15784/601405. AMD - DIF Record(s) USAP-1341602_1 Abstract Studies in temperate fishes provide evidence that cardiac mitochondrial function and the capacity to fuel cardiac work contribute to thermal tolerance. Here, we tested the hypothesis that decreased cardiac aerobic metabolic capacity contributes to the lower thermal tolerance of the haemoglobinless Antarctic icefish, Chaenocephalus aceratus, compared with that of the red-blooded Antarctic species, Notothenia coriiceps. Maximal activities of citrate synthase (CS) and lactate dehydrogenase (LDH), respiration rates of isolated mitochondria, adenylate levels and changes in mitochondrial protein expression were quantified from hearts of animals held at ambient temperature or exposed to their critical thermal maximum (CTmax). Compared with C. aceratus, activity of CS, ATP concentration and energy charge were higher in hearts of N. coriiceps at ambient temperature and CTmax While state 3 mitochondrial respiration rates were not impaired by exposure to CTmax in either species, state 4 rates, indicative of proton leakage, increased following exposure to CTmax in C. aceratus but not N. coriiceps The interactive effect of temperature and species resulted in an increase in antioxidants and aerobic metabolic enzymes in N. coriiceps but not in C. aceratus Together, our results support the hypothesis that the lower aerobic metabolic capacity of C. aceratus hearts contributes to its low thermal tolerance. Creator(s): O'Brien, Kristin Date Created: 2020-12-18 Repository: USAP-DC (current) License: Creative Commons Attribution Only v4.0 Generic [CC BY 4.0] Award(s) NSF 1341663 Version: 1 Related Project(s) Collaborative Research: The Physiological and Biochemical Underpinnings of Thermal Tolerance in Antarctic Notothenioid Fishes References O’Brien, K.M., A.S. Rix, S. Egginton, A.P. Farrell, E.L. Crockett, K. Schlauch, R. Woolsey, M. Hoffman, and S. Merriman. (2018). Cardiac mitochondrial metabolism may contribute to differences in thermal tolerance of red- and white-blooded Antarctic notothenioid fishes. Journal of Experimental Biology 221. jeb 177816. (doi:10.1242/jeb.177816) Keywords Antarctic Peninsula Antarctica