USAP-DC

{"dp_type": "Project", "free_text": "Adaptation"}

[{"awards": "2038149 Warner, Jacob", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": null, "datasets": null, "date_created": "Wed, 09 Oct 2024 00:00:00 GMT", "description": "Antarctic marine invertebrates exhibit extraordinarily slow rates of development. This phenomenon has arisen repeatedly in independent Antarctic lineages, including sea urchins, sea stars, brachiopods, and ribbon worms. Despite these observations, little is known about the molecular mechanisms responsible for slow developmental rates in Antarctic marine invertebrates. This proposal is developing the Antarctic sea urchin, Sterechinus neumayeri, as a model invertebrate organism to evaluate cold water organismal adaptation and development. Urchins collected from McMurdo Sound are being studied in carefully controlled laboratory experiments. This work is specifically identifying the gene regulatory network components responsible for regulating developmental timing in S. neumayeri and, more generally, which gene regulatory network elements evolved during adaption to the extreme environment of the Southern Ocean. The lab-based work is focusing in two specific areas: 1) Identify unique gene regulatory network components of S. neumayeri that evolved during its developmental adaptation to the Southern Ocean, and 2) Analyze spatial expression and functions of key genes in the early S. neumayeri gene regulatory networks controlling specification and patterning of territories along the early anterior-posterior axis. A comparative analysis of better studied urchins from warmer regions will be used to inform this work. This effort is relevant to several fields of biology ranging from polar biology, developmental biology, evolution, and genomics while explicitly tying genotype to phenotype. Broader impacts: The proposal included three early career investigators who are new to Antarctic research programs working alongside a well-established Antarctic investigator. The team has developed an ambitious program for science and technology training in computer coding and biology targeted for underrepresented students. They also have developed web-based bioinformatics training blog, \u201c2-bitbio\u201d, which aims to decrease the \u2018barrier to entry\u2019 into the field of bioinformatics. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "Antarctica; ECHINODERMS", "locations": "Antarctica", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Warner, Jacob", "platforms": null, "repositories": null, "science_programs": null, "south": -90.0, "title": "Collaborative Research: LIA: Genomic Mechanisms Controlling the Slow Development of the Antarctic Urchin Sterechinus Neumayeri", "uid": "p0010480", "west": -180.0}, {"awards": "2317995 Herman, Rachael", "bounds_geometry": "POLYGON((-65 -61,-64 -61,-63 -61,-62 -61,-61 -61,-60 -61,-59 -61,-58 -61,-57 -61,-56 -61,-55 -61,-55 -61.5,-55 -62,-55 -62.5,-55 -63,-55 -63.5,-55 -64,-55 -64.5,-55 -65,-55 -65.5,-55 -66,-56 -66,-57 -66,-58 -66,-59 -66,-60 -66,-61 -66,-62 -66,-63 -66,-64 -66,-65 -66,-65 -65.5,-65 -65,-65 -64.5,-65 -64,-65 -63.5,-65 -63,-65 -62.5,-65 -62,-65 -61.5,-65 -61))", "dataset_titles": null, "datasets": null, "date_created": "Fri, 04 Oct 2024 00:00:00 GMT", "description": "Gentoo penguins (Pygoscelis papua) inhabit one of the fastest warming regions on Earth, the Western Antarctic Peninsula (WAP), where environmental shifts are measured in years, not decades. Despite this, the species is flourishing, growing in numbers and colonizing new habitats while sister species, such as Ad\u00e9lie penguins (P. adeliae), are declining in the region. This project will investigate to what extent epigenetics contributes to the success of gentoo penguins. Epigenetic variation is controlled by modifications to DNA or chromatin structure that affect the expression of genes, rather than changes to the underlying DNA sequence. This project will improve the understanding of gentoo penguin adaptation to climate change, and whether it is a result of increased flexibility in behavior and physiology driven by a greater capacity for epigenetic changes (i.e., epigenetic potential). The most studied form of epigenetic variation is the profiling of DNA methylation patterns. Environmental effects can trigger changes in DNA methylation that target specific tissues, allowing for localized gene expression shifts that result in modifications to the phenotype of an organism without any alteration to the underlying genotype. Given that epigenetic variation between populations often exceeds genetic variation, fine-scale genetic differentiation observed amongst gentoo penguin colonies suggests the possibility for local adaptation via even more divergent epigenetic changes and provides a framework for examining epigenetic variation across the gentoo penguin breeding range along multiple ecological axes. The researchers will test this by comprehensively characterizing the epigenomic profiles via patterns of DNA methylation in wild gentoo and Ad\u00e9lie penguins using cutting-edge high-resolution genomics techniques. Specifically, they will investigate whether gentoo penguins exhibit a greater degree of differences in DNA-methylation than underlying genetic differences, suggesting such epigenetic variation is driven by external environmental variables, potentially leading to improved capacity for local adaptation. This project will explore whether epigenetic potential may be selected for in individuals who disperse to new colony locations by comparing older, established colonies to new colonies at the range-edge. By implementing cutting-edge epigenetic methods in wild populations of gentoo penguins, this project will help address ecological questions on environmental plasticity that will impact conservation efforts and decisions on Marine Protected Areas (MPAs) on the Antarctic Peninsula. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": -55.0, "geometry": "POINT(-60 -63.5)", "instruments": null, "is_usap_dc": true, "keywords": "Gentoo Penguin; ECOLOGICAL DYNAMICS; Adaptation; Methylation; Antarctic Peninsula; Climate Change; Epigenetic Variation; PENGUINS", "locations": "Antarctic Peninsula", "north": -61.0, "nsf_funding_programs": "Post Doc/Travel; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Herman, Rachael", "platforms": null, "repositories": null, "science_programs": null, "south": -66.0, "title": "Postdoctoral Fellowship: OPP-PRF: Epigenetic Potential as a Driver of Local Adaptation in Gentoo Penguins (Pygoscelis Papua) along the Western Antarctic Peninsula", "uid": "p0010477", "west": -65.0}, {"awards": "1955368 Daane, Jacob; 2324998 Daane, Jacob", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": null, "datasets": null, "date_created": "Thu, 01 Aug 2024 00:00:00 GMT", "description": "Part I: Nontechnical description The ecologically important notothenioid fish of the Southern Ocean surrounding Antarctica will be studied to address questions central to polar, evolutionary, and adaptational biology. The rapid diversification of the notothenioids into \u003e120 species following a period of Antarctic glaciation and cooling of the Southern Ocean is thought to have been facilitated by key evolutionary innovations, including antifreeze glycoproteins to prevent freezing and bone reduction to increase buoyancy. In this project, a large dataset of genomic sequences will be used to evaluate the genetic mechanisms that underly the broad pattern of novel trait evolution in these fish, including traits relevant to human diseases (e.g., bone density, renal function, and anemia). The team will develop new STEM-based research and teaching modules for undergraduate education at Northeastern University. The work will provide specific research training to scholars at all levels, including a post-doctoral researcher, a graduate student, undergraduate students, and high school students. The team will also contribute to public outreach, including, in part, the develop of teaching videos in molecular evolutionary biology and accompanying educational supplements. Part II: Technical description The researchers will leverage their comprehensive notothenioid phylogenomic dataset comprising \u003e250,000 protein-coding exons and conserved non-coding elements across 44 ingroup and 2 outgroup species to analyze the genetic origins of three iconic notothenioid traits: (1) loss of erythrocytes by the icefish clade in a cold, stable and highly-oxygenated marine environment; (2) reduction in bone mass and retention of juvenile skeletal characteristics as buoyancy mechanisms to facilitate foraging; and (3) loss of kidney glomeruli to retain energetically expensive antifreeze glycoproteins. The team will first track patterns of change in erythroid-related genes throughout the notothenioid phylogeny. They will then examine whether repetitive evolution of a pedomorphic skeleton in notothenioids is based on parallel or divergent evolution of genetic regulators of heterochrony. Third, they will determine whether there is mutational bias in the mechanisms of loss and re-emergence of kidney glomeruli. Finally, identified genetic mechanisms of evolutionary change will be validated by experimental testing using functional genomic strategies in the zebrafish model system. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "United States Of America; FISH", "locations": "United States Of America", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Daane, Jacob; Detrich, H. William", "platforms": null, "repositories": null, "science_programs": null, "south": -90.0, "title": "ANT LIA: Collaborative Research: Evolutionary Patterns and Mechanisms of Trait Diversification in the Antarctic Notothenioid Radiation", "uid": "p0010473", "west": -180.0}, {"awards": "2341344 Baker, Bill", "bounds_geometry": null, "dataset_titles": null, "datasets": null, "date_created": "Fri, 15 Sep 2023 00:00:00 GMT", "description": "Non-technical project description Museums of natural history, such as the Smithsonian National Museum of Natural History (NMNH) in Washington, D.C., are repositories for, among other things, biological specimens. Specimens stored at the NMNH were obtained over many decades and across the globe, resulting in what is currently a treasure trove of biological and chemical information. Chemical compounds (metabolites) found in the tissues of, for example, marine invertebrates, can record the organism\u2019s response to a changing environment. This project seeks to establish a strategy for analyzing these compounds in Antarctic marine invertebrates held in the NMNH. These organisms are especially valuable for studies of their metabolites as such information will contribute to our understanding of the history of the polar environment and how organisms are able to adapt to extreme habitats. Further, studies of these rare and difficult to obtain metabolites have broad impacts in biotechnology and human health. Technical description of the project This project seeks to develop a workflow for the analysis of metabolites in archival marine invertebrate specimens held in the Smithsonian National Museum of Natural History (NMNH). Recent advances in mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy, both instrumental as well as analysis platforms, enable the detection and annotation of chemical structures in these otherwise difficult to obtain metabolites. In particular, NMR strategy (Pure Shift NMR) will be implemented to increase sensitivity toward these sample-limited analytes. Further, the workflow will be applied in an analysis of storage methods used by the NMNH with the aim of understanding how best to preserve specimens for future metabolomics analyses. With an optimized workflow established, additional applications to inform our understanding of adaptation and (cryptic) speciation in the extreme habitats found in Antarctica are possible. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "Antarctica; ANIMALS/INVERTEBRATES", "locations": "Antarctica", "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Baker, Bill", "platforms": null, "repositories": null, "science_programs": null, "south": null, "title": "EAGER: Metabolomics Analysis of Archival Marine Invertebrates", "uid": "p0010435", "west": null}, {"awards": "2228257 Michaud, Alexander", "bounds_geometry": "POINT(-112.05 -79.28)", "dataset_titles": null, "datasets": null, "date_created": "Wed, 31 May 2023 00:00:00 GMT", "description": "Ice cores from glaciers and ice sheets provide detailed archives of past environmental conditions, furthering our understanding of Earth\u2019s climate. Microorganisms in the West Antarctic Ice Sheet are buried over glaciological time and form a stratigraphy record providing the opportunity of analysis of the order and position of layers of geological events, with potential links to Southern Hemisphere climate. However, microbial cells that land on the ice sheet are subject to the stresses of changing habitat conditions due to burial and conditions associated with long-term isolation in ice. These processes may lead to a loss of fidelity within the stratigraphic record of microbial cells. We know little about how and if microorganisms survive burial and remain alive over glacial-interglacial time periods within an ice sheet. This analysis will identify the viable and preserved community of microorganisms and core genomic adaptation that permit cell viability, which will advance knowledge in the areas of microbiology and glaciology while increasing fidelity of ice core measurements relevant to past climate and potential future global climate impacts. This exploratory endeavor has the potential to be a transformative step toward understanding the ecology of one of the most understudied environments on Earth. The project will partner with the Museum of Science, Boston, to increase public scientific literacy via education and outreach. Additionally, this project will support two early-career scientists and two undergraduates in interdisciplinary research at the intersection of microbiology and climate science. Results from this project will provide the first DNA data based on single-cell whole genomic sequencing from the Antarctic Ice Sheet and inform whether post-depositional processes impact the interpretations of paleoenvironmental conditions from microbes. The goals to determine the taxonomic identity of viable and preserved microbial cells, and decode the genetic repertoire that confers survival of burial and long-term viability within glacial ice, will be achieved by utilizing subsamples from a ~60,000 year old record of the West Antarctic Ice Sheet Divide (WD) Ice Core. WD samples will be melted using the Desert Research Institute\u2019s ice core melting system that is optimized for glaciobiological sampling. Microbial cells from the meltwater will be sorted using fluorescence-activated cell sorting, and individually sorted cells will have their genomes sequenced. The fluorescence-based methods will discern the viable (metabolically active) cells from those cells that are non-viable but preserved in the ice (DNA-containing). The genomic analysis will identify the taxonomy of each cell, presence of known genes that confer survival in permanently frozen environments, and comparatively analyze genomes to determine the core set of genes required by viable cells to persist in an ice sheet. The outcomes of this work will expand the potential for biological measurements and contamination control from archived ice cores. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": -112.05, "geometry": "POINT(-112.05 -79.28)", "instruments": null, "is_usap_dc": true, "keywords": "WAIS Divide; TERRESTRIAL ECOSYSTEMS; ICE SHEETS; BACTERIA/ARCHAEA; ICE CORE RECORDS", "locations": "WAIS Divide", "north": -79.28, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Michaud, Alexander; Winski, Dominic A.", "platforms": null, "repositories": null, "science_programs": null, "south": -79.28, "title": "EAGER: ANT LIA: Persist or Perish: Records of Microbial Survival and Long-term Persistence from the West Antarctic Ice Sheet", "uid": "p0010421", "west": -112.05}, {"awards": "1644256 Costa, Daniel; 1643575 Kanatous, Shane; 1644004 Trumble, Stephen", "bounds_geometry": "POLYGON((-66.534369 -52.962091,-65.3857434 -52.962091,-64.2371178 -52.962091,-63.0884922 -52.962091,-61.9398666 -52.962091,-60.791241 -52.962091,-59.6426154 -52.962091,-58.4939898 -52.962091,-57.3453642 -52.962091,-56.1967386 -52.962091,-55.048113 -52.962091,-55.048113 -54.530129,-55.048113 -56.098167000000004,-55.048113 -57.666205000000005,-55.048113 -59.234243,-55.048113 -60.802281,-55.048113 -62.370319,-55.048113 -63.938357,-55.048113 -65.506395,-55.048113 -67.074433,-55.048113 -68.642471,-56.1967386 -68.642471,-57.3453642 -68.642471,-58.4939898 -68.642471,-59.6426154 -68.642471,-60.791241 -68.642471,-61.9398666 -68.642471,-63.0884922 -68.642471,-64.2371178 -68.642471,-65.3857434 -68.642471,-66.534369 -68.642471,-66.534369 -67.074433,-66.534369 -65.506395,-66.534369 -63.938356999999996,-66.534369 -62.370319,-66.534369 -60.802281,-66.534369 -59.234243,-66.534369 -57.666205,-66.534369 -56.098167000000004,-66.534369 -54.530129,-66.534369 -52.962091))", "dataset_titles": "Data from: Whiskers provide time-series of toxic and essential trace elements, Se:Hg molar ratios, and stable isotope values of an apex Antarctic predator, the leopard seal; Leopard Seal Diving behavior data; Leopard Seal movement data", "datasets": [{"dataset_uid": "601689", "doi": "10.15784/601689", "keywords": "Antarctica; Antarctic Peninsula; Biota; Body Mass; Diving Behavior; Leopard Seal; Movement Data; Seals", "people": "Costa, Daniel", "repository": "USAP-DC", "science_program": null, "title": "Leopard Seal movement data", "url": "https://www.usap-dc.org/view/dataset/601689"}, {"dataset_uid": "200361", "doi": "https://doi.org/10.5061/dryad.ksn02v75b", "keywords": null, "people": null, "repository": "Dryad", "science_program": null, "title": "Data from: Whiskers provide time-series of toxic and essential trace elements, Se:Hg molar ratios, and stable isotope values of an apex Antarctic predator, the leopard seal", "url": "https://datadryad.org/stash/dataset/doi:10.5061%2Fdryad.ksn02v75b"}, {"dataset_uid": "601690", "doi": "10.15784/601690", "keywords": "Antarctica; Antarctic Peninsula; Biota; Body Mass; Diving Behavior; Leopard Seal; Seals", "people": "Costa, Daniel", "repository": "USAP-DC", "science_program": null, "title": "Leopard Seal Diving behavior data", "url": "https://www.usap-dc.org/view/dataset/601690"}], "date_created": "Fri, 12 May 2023 00:00:00 GMT", "description": "This research project is a multidisciplinary effort that brings together a diverse team of scientists from multiple institutions together to understand the foraging behavior and physiology of leopard seals and their role in the Southern Ocean food web. The project will examine the physiology and behavior of leopard seals to in an effort to determine their ability to respond to potential changes in their habitat and foraging areas. Using satellite tracking devices the team will examine the movement and diving behavior of leopard seals and couple this information with measurements of their physiological capacity. The project will determine whether leopard seals- who feed on diverse range of prey- are built differently than their deep diving relatives the Weddell and elephant seal who feed on fish and squid. The team will also determine whether leopard seals are operating at or near their physiological capability to determine how much, if any, ?reserve capacity? they might have to forage and live in changing environments. A better understanding of their home ranges, movement patterns, and general behavior will also be informative to help in managing human-leopard seal interactions. The highly visual nature of the data and analysis for this project lends itself to public and educational display and outreach, particularly as they relate to the changing Antarctic habitats. The project will use the research results to educate the public on the unique physiological and ecological adaptations to extreme environments seen in diving marine mammals, including adaptations to exercise under low oxygen conditions and energy utilization, which affect and dictate the lifestyle of these exceptional organisms. The results of the project will also contribute to the broader understanding that may enhance the aims of managing marine living resources. The leopard seal is an apex predator in the Antarctic ecosystem. This project seeks to better understand the ability of the leopard seal to cope with a changing environment. The project will first examine the foraging behavior and habitat utilization of leopard seals using satellite telemetry. Specifically, satellite telemetry tags will be used to obtain dive profiles and movement data for individuals across multiple years. Diet and trophic level positions across multiple temporal scales will then be determined from physiological samples (e.g., blood, vibrissae, blubber fatty acids, stable isotopes, fecal matter). Oceanographic data will be integrated with these measures to develop habitat models that will be used to assess habitat type, habitat utilization, habitat preference, and home range areas for individual animals. Diet composition for individual seals will be evaluated to determine whether specific animals are generalists or specialists. Second, the team will investigate the physiological adaptations that allow leopard seals to be apex predators and determine to what extent leopard seals are working at or near their physiological limit. Diving behavior and physiology of leopard seals will be evaluated (for instance the aerobic dive limit for individual animals and skeletal muscle adaptations will be determined for diving under hypoxic conditions). Data from time-depth recorders will be used to determine foraging strategies for individual seals, and these diving characteristics will be related to physiological variables (e.g., blood volume, muscle oxygen stores) to better understand the link between foraging behavior and physiology. The team will compare myoglobin storage in swimming muscles associated with both forelimb and hind limb propulsion and the use of anaerobic versus aerobic metabolic systems while foraging.", "east": -55.048113, "geometry": "POINT(-60.791241 -60.802281)", "instruments": null, "is_usap_dc": true, "keywords": "Antarctic Peninsula; Diving Behavior; MAMMALS; MARINE ECOSYSTEMS; Movement Patterns; Leopard Seal", "locations": "Antarctic Peninsula", "north": -52.962091, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Costa, Daniel; Trumble, Stephen J; Kanatous, Shane", "platforms": null, "repo": "USAP-DC", "repositories": "Dryad; USAP-DC", "science_programs": null, "south": -68.642471, "title": "Collaborative Research: Foraging Ecology and Physiology of the Leopard Seal", "uid": "p0010419", "west": -66.534369}, {"awards": "2232891 Postlethwait, John; 1947040 Postlethwait, John; 1543383 Postlethwait, John", "bounds_geometry": "POLYGON((-180 -37,-144 -37,-108 -37,-72 -37,-36 -37,0 -37,36 -37,72 -37,108 -37,144 -37,180 -37,180 -42.3,180 -47.6,180 -52.9,180 -58.2,180 -63.5,180 -68.8,180 -74.1,180 -79.4,180 -84.69999999999999,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -84.7,-180 -79.4,-180 -74.1,-180 -68.8,-180 -63.5,-180 -58.2,-180 -52.9,-180 -47.6,-180 -42.300000000000004,-180 -37))", "dataset_titles": "aBSREL tests for episodic diversifying selection on hemoglobin genes in notothenioids.; MEME tests of sites evolving under episodic diversifying selection in notothenioid hemoglobin genes.; Notothenioid hemoglobin protein 3D modeling.; Notothenioid species tree used in the study.; Phylogenetic trees of hemoglobin proteins in notothenioids.; Rates of hemoglobin evolution among genes and across notothenioid species.; RELAX tests for pervasive changes in strength of natural selection on hemoglobin genes in notothenioids.", "datasets": [{"dataset_uid": "601728", "doi": "10.15784/601728", "keywords": "Antarctica; Cold Adaptation; Cryonotothenioid; Dragonfish; Eleginopsioidea; Fish; Gene; Genetic Analysis; Hemoglobin; Icefish; Notothenioid; Notothenioid Fishes; Plunderfish; Sub-Antarctic", "people": "Postlethwait, John; Desvignes, Thomas", "repository": "USAP-DC", "science_program": null, "title": "aBSREL tests for episodic diversifying selection on hemoglobin genes in notothenioids.", "url": "https://www.usap-dc.org/view/dataset/601728"}, {"dataset_uid": "601732", "doi": "10.15784/601732", "keywords": "Antarctica; Cold Adaptation; Cryonotothenioid; Dragonfish; Eleginopsioidea; Fish; Genetic Analysis; Hemoglobin; Icefish; Notothenioid; Notothenioid Fishes; Plunderfish; Sub-Antarctic", "people": "Desvignes, Thomas; Postlethwait, John", "repository": "USAP-DC", "science_program": null, "title": "Notothenioid hemoglobin protein 3D modeling.", "url": "https://www.usap-dc.org/view/dataset/601732"}, {"dataset_uid": "601731", "doi": "10.15784/601731", "keywords": "Antarctica; Cold Adaptation; Cryonotothenioid; Dragonfish; Eleginopsioidea; Fish; Genetic Analysis; Hemoglobin; Icefish; Notothenioid; Notothenioid Fishes; Plunderfish; Sub-Antarctic", "people": "Postlethwait, John; Desvignes, Thomas", "repository": "USAP-DC", "science_program": null, "title": "RELAX tests for pervasive changes in strength of natural selection on hemoglobin genes in notothenioids.", "url": "https://www.usap-dc.org/view/dataset/601731"}, {"dataset_uid": "601721", "doi": "10.15784/601721", "keywords": "Antarctica; Cold Adaptation; Cryonotothenioid; Dragonfish; Eleginopsioidea; Fish; Gene; Hemoglobin; Icefish; Notothenioid; Plunderfish; Sub-Antarctic", "people": "Desvignes, Thomas; Postlethwait, John", "repository": "USAP-DC", "science_program": null, "title": "Notothenioid species tree used in the study.", "url": "https://www.usap-dc.org/view/dataset/601721"}, {"dataset_uid": "601722", "doi": "10.15784/601722", "keywords": "Antarctica; Cold Adaptation; Cryonotothenioid; Dragonfish; Eleginopsioidea; Fish; Gene; Hemoglobin; Icefish; Notothenioid; Plunderfish; Sub-Antarctic", "people": "Desvignes, Thomas; Postlethwait, John", "repository": "USAP-DC", "science_program": null, "title": "Phylogenetic trees of hemoglobin proteins in notothenioids.", "url": "https://www.usap-dc.org/view/dataset/601722"}, {"dataset_uid": "601730", "doi": "10.15784/601730", "keywords": "Antarctica; Cold Adaptation; Cryonotothenioid; Dragonfish; Eleginopsioidea; Fish; Genetic Analysis; Hemoglobin; Icefish; Notothenioid; Notothenioid Fishes; Plunderfish; Sub-Antarctic", "people": "Postlethwait, John; Desvignes, Thomas", "repository": "USAP-DC", "science_program": null, "title": "MEME tests of sites evolving under episodic diversifying selection in notothenioid hemoglobin genes.", "url": "https://www.usap-dc.org/view/dataset/601730"}, {"dataset_uid": "601729", "doi": "10.15784/601729", "keywords": "Antarctica; Cold Adaptation; Cryonotothenioid; Dragonfish; Eleginopsioidea; Fish; Genetic Analysis; Hemoglobin; Icefish; Notothenioid; Notothenioid Fishes; Plunderfish; Sub-Antarctic", "people": "Desvignes, Thomas; Postlethwait, John", "repository": "USAP-DC", "science_program": null, "title": "Rates of hemoglobin evolution among genes and across notothenioid species.", "url": "https://www.usap-dc.org/view/dataset/601729"}], "date_created": "Wed, 03 May 2023 00:00:00 GMT", "description": "Antarctic notothenioid fishes, also known as cryonotothenioids, inhabit the icy and highly oxygenated waters surrounding the Antarctic continent after diverging from notothenioids inhabiting more temperate waters. Notothenioid hemoglobin and blood parameters are known to have evolved along with the establishment of stable polar conditions, and among Antarctic notothenioids, icefishes are evolutionary oddities living without hemoglobin following the deletion of all functional hemoglobin genes from their genomes. In this project, we investigate the evolution of hemoglobin genes and gene clusters across the notothenioid radiation until their loss in the icefish ancestor after its divergence from the dragonfish lineage to understand the forces, mechanisms, and potential causes for hemoglobin gene loss in the icefish ancestor.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "FISH; Icefish; Cryonotothenioid; Gene; Plunderfish; Eleginopsioidea; AQUATIC ECOSYSTEMS; Dragonfish; Sub-Antarctic; Notothenioid; Blood; Hemoglobin", "locations": "Sub-Antarctic", "north": -37.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Desvignes, Thomas; Postlethwait, John", "platforms": null, "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -90.0, "title": "Evolution of hemoglobin genes in notothenioid fishes", "uid": "p0010417", "west": -180.0}, {"awards": "2133684 Fierer, Noah; 2133685 Adams, Byron", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "Geochemical, physicochemical, and genomic data from a continental-scale survey of microbial diversity in Antarctic soils", "datasets": [{"dataset_uid": "200478", "doi": "10.6073/pasta/b4858653c587864f0111aba4c3014d61", "keywords": null, "people": null, "repository": "EDI", "science_program": null, "title": "Geochemical, physicochemical, and genomic data from a continental-scale survey of microbial diversity in Antarctic soils", "url": "https://portal.edirepository.org/nis/mapbrowse?packageid=knb-lter-mcm.275.1"}], "date_created": "Fri, 07 Apr 2023 00:00:00 GMT", "description": "Not all of Antarctica is covered in ice. In fact, soils are common to many parts of Antarctica, and these soils are often unlike any others found on Earth. Antarctic soils harbor unique microorganisms able to cope with the extremely cold and dry conditions common to much of the continent. For decades, microbiologists have been drawn to the unique soils in Antarctica, yet critical knowledge gaps remain. Most notably, it is unclear what properties allow certain microbes to thrive in Antarctic soils. By using a range of methods, this project is developing comprehensive model that discovers the unique genomic features of soils diversity, distributions, and adaptations that allow Antarctic soil microbes to thrive in extreme environments. The proposed work will be relevant to researchers in many fields, including engineers seeking to develop new biotechnologies, ecologists studying the contributions of these microbial communities to the functioning of Antarctic ecosystems, microbiologists studying novel microbial adaptations to extreme environmental conditions, and even astrobiologists studying the potential for life on Mars. More generally, the proposed research presents an opportunity to advance our current understanding of the microbial life found in one of the more distinctive microbial habitats on Earth, a habitat that is inaccessible to many scientists and a habitat that is increasingly under threat from climate change. The research project explores the microbial diversity in Antarctic soils and links specific features to different soil types and environmental conditions. The overarching questions include: What microbial taxa are found in a variety of Antarctic environments? What are the environmental preferences of specific taxa or lineages? What are the genomic and phenotypic traits of microorganisms that allow them to persist in extreme environments and determine biogeographical differneces? This project will analyze archived soils collected from across Antarctica by a network of international collaborators, with samples selected to span broad gradients in soil and site conditions. The project uses cultivation-independent, high-throughput genomic analysis methods and cultivation-dependent approaches to analyze bacterial and fungal communities in soil samples. The results will be used to predict the distributions of specific taxa and lineages, obtain genomic information for the more ubiquitous and abundant taxa, and quantify growth responses in vitro across gradients in temperature, moisture, and salinity. This integration of ecological, environmental, genomic, and trait-based information will provide a comprehensive understanding of microbial life in Antarctic soils. This project will also help facilitate new collaborations between scientists across the globe while providing undergraduate students with \u0027hands-on\u0027 research experiences that introduce the next generation of scientists to the field of Antarctic biology. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "FUNGI; BACTERIA/ARCHAEA; TERRESTRIAL ECOSYSTEMS; Antarctica", "locations": "Antarctica", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Fierer, Noah; Quandt, Alisha A; Adams, Byron; Lemonte, Joshua", "platforms": null, "repo": "EDI", "repositories": "EDI", "science_programs": null, "south": -90.0, "title": "Collaborative Research: ANT LIA Integrating Genomic and Phenotypic Analyses to understand Microbial Life in Antarctic Soils", "uid": "p0010414", "west": -180.0}, {"awards": "2149070 Hawco, Nicholas", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": null, "datasets": null, "date_created": "Mon, 13 Mar 2023 00:00:00 GMT", "description": "The current understanding of what controls productivity in the Southern Ocean is based mostly on the scarcity of a metal compound needed for algal growth, Dissolved Iron in seawater. There is growing evidence that Manganese also plays a critical role in maintaining algal growth and if found in low concentrations can play a role in limiting primary productivity. As algal growth is a major player in absorbing carbon dioxide from the atmosphere, understanding what controls productivity increases our understanding of what role the Southern Ocean plays in the global carbon cycle. This study proposes to study the algal processes that take up Manganese in Antarctic diatoms, one of the main primary producers in the region. Another aspect will be to understand how Zinc, a micronutrient with similar dynamics than Manganese, can inhibit its uptake. The PIs propose lab experiments with cultured diatoms isolated from the Southern Ocean to obtain answers to their questions on micronutrient dynamics and will compare results from those obtained with a diatom species isolated from temperate waters. The proposed research will benefit NSF\u2019s goals of understanding life in cold environments and how they differ from other parts of the ocean. This project will support two first-time early career scientists and a female researcher in Earth Sciences. Two graduate students will also be supported, and scientific techniques used in this research will be shared at open houses sponsored by the academic institutions and with local summer schools. This proposal represents collaborative research to explore manganese (Mn) limitation in Antarctic diatoms by two early career investigators. Diatoms are central players in the Southern Ocean carbon cycle, where the micronutrient chemistry is fundamentally different from other oceans. The Southern Ocean is characterized by widespread low Mn, coupled with high zinc (Zn). High Zn levels are potentially toxic to diatoms as Zn can competitively inhibit Mn uptake and metabolism, compromising the ability of building critical cellular components, thus impacting the biological pump. Using culture experiments with a matrix of micronutrient treatments (Mn, Zn, Fe) and irradiances, and using physiological and transcriptomic approaches, along with biochemical principles, the Principal Investigators will address the central hypothesis (diatoms from the Southern Ocean possess physiological mechanisms to low Mn/high Zn) to quantify rates of uptake and transporter binding constants. The transcriptomics approach will help to identify candidate genes that may provide Antarctic diatoms physiological mechanisms in low Mn/high Zn environment. The project does not require fieldwork but instead would make use of culture experiments with 4 diatom species (3 Antarctic, and 1 temperate). The proposed approach will also enable the goal of developing biomarker(s) for assessing Mn stress or Zn toxicity and results from the physiological experiments will help parameterize models of micronutrient limitation in the Southern Ocean. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "Southern Ocean; BIOGEOCHEMICAL CYCLES; TRACE ELEMENTS; DIATOMS; Iron; Phytoplankton", "locations": "Southern Ocean", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Hawco, Nicholas; Cohen, Natalie", "platforms": null, "repositories": null, "science_programs": null, "south": -90.0, "title": "ANT LIA: Collaborative Research: Adaptations of Southern Ocean Diatoms to Manganese Scarcity: Can Physiological Ingenuity Overcome Unfavorable Chemistry?", "uid": "p0010412", "west": -180.0}, {"awards": "2240780 Cohen, Natalie", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": null, "datasets": null, "date_created": "Mon, 13 Mar 2023 00:00:00 GMT", "description": "Mixotrophs are essential components of the Antarctic planktonic community able to photosynthesize and also ingest small particles like bacteria to meet their nutritional needs. This project aims to understand the physiological response of mixotrophs exposed to micronutrient limitation in the Southern Ocean, specifically iron, manganese and simultaneous limitation of more than one trace metal, or colimitation. Such environmental conditions are characteristic of the Southern Ocean and can only be tested with local algae. The Principal Investigators hypothesize that under trace metal colimitation, some mixotrophs will have a competitive advantage by increasing their ability to consume particles to obtain energy and trace metals from their prey. Given the lack of understanding of how mixotrophs have adapted to the micronutrient limitation, the researchers propose studies with microalgal cultures isolated from the Southern Ocean; they will measure growth responses, consumption behavior, changes in cellular chemistry and transcription of genetic material in response to iron and manganese limitation. This project benefits the National Science Foundation goals of understanding Life in Antarctica and adaptation of organisms to this extreme environment. Society will benefit from the training proposed, whereby students from rural colleges will be instructed in computer coding and scientific data analyses. Furthermore, this work will support one graduate student, two undergraduate summer interns, and two early career scientists. The Principal Investigators hypothesize that under Fe-Mn colimitation, some mixotrophs will have a competitive advantage by increasing their grazing rates to obtain energy, Fe, and Mn from their prey. Given the lack of understanding of how mixotrophs have adapted to seasonal changes in the availability of these micronutrients and how they influence mixotrophic growth dynamics, the PIs propose culture studies to measure growth responses, grazing behavior, and changes in elemental stoichiometry in response to Fe and Mn limitation. Transcriptomic analyses will reveal the metabolic underpinnings of trophic behavior and micronutrient stress responses, with implications for key biogeochemical processes such as carbon fixation, remineralization, and nutrient cycling. Results are expected to clarify the ecological roles of Antarctic mixotrophs and elucidate the adaptations of Southern Ocean organisms to their unique polar ecosystem following the 2015 Strategic Vision for Polar Programs. This work will support one graduate student, two undergraduate summer interns, and two early career scientists. A series of virtual coding and bioinformatic workshops will be organized, in which basic principles of coding, and data processing used in the proposed analysis will be taught to undergraduate students. Small colleges in rural areas will be targeted for 8 modules on bioinformatics training. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "PLANKTON; Georgia; PHYTOPLANKTON", "locations": "Georgia", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Cohen, Natalie; Millette, Nicole", "platforms": null, "repositories": null, "science_programs": null, "south": -90.0, "title": "ANT LIA: Collaborative Research: Mixotrophic Grazing as a Strategy to meet Nutritional Requirements in the Iron and Manganese Deficient Southern Ocean", "uid": "p0010411", "west": -180.0}, {"awards": "1644004 Trumble, Stephen", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "Data from: Whiskers provide time-series of toxic and essential trace elements, Se:Hg molar ratios, and stable isotope values of an apex Antarctic predator, the leopard seal", "datasets": [{"dataset_uid": "200338", "doi": "doi:10.5061/dryad.ksn02v75b", "keywords": null, "people": null, "repository": "Dryad", "science_program": null, "title": "Data from: Whiskers provide time-series of toxic and essential trace elements, Se:Hg molar ratios, and stable isotope values of an apex Antarctic predator, the leopard seal", "url": "https://datadryad.org/stash/share/h6UwXvfhZG26jtPTtDqyXNMnx2UWknOqmv05EBz6A10"}], "date_created": "Tue, 06 Dec 2022 00:00:00 GMT", "description": "This research project is a multidisciplinary effort that brings together a diverse team of scientists from multiple institutions together to understand the foraging behavior and physiology of leopard seals and their role in the Southern Ocean food web. The project will examine the physiology and behavior of leopard seals to in an effort to determine their ability to respond to potential changes in their habitat and foraging areas. Using satellite tracking devices the team will examine the movement and diving behavior of leopard seals and couple this information with measurements of their physiological capacity. The project will determine whether leopard seals- who feed on diverse range of prey- are built differently than their deep diving relatives the Weddell and elephant seal who feed on fish and squid. The team will also determine whether leopard seals are operating at or near their physiological capability to determine how much, if any, ?reserve capacity? they might have to forage and live in changing environments. A better understanding of their home ranges, movement patterns, and general behavior will also be informative to help in managing human-leopard seal interactions. The highly visual nature of the data and analysis for this project lends itself to public and educational display and outreach, particularly as they relate to the changing Antarctic habitats. The project will use the research results to educate the public on the unique physiological and ecological adaptations to extreme environments seen in diving marine mammals, including adaptations to exercise under low oxygen conditions and energy utilization, which affect and dictate the lifestyle of these exceptional organisms. The results of the project will also contribute to the broader understanding that may enhance the aims of managing marine living resources. The leopard seal is an apex predator in the Antarctic ecosystem. This project seeks to better understand the ability of the leopard seal to cope with a changing environment. The project will first examine the foraging behavior and habitat utilization of leopard seals using satellite telemetry. Specifically, satellite telemetry tags will be used to obtain dive profiles and movement data for individuals across multiple years. Diet and trophic level positions across multiple temporal scales will then be determined from physiological samples (e.g., blood, vibrissae, blubber fatty acids, stable isotopes, fecal matter). Oceanographic data will be integrated with these measures to develop habitat models that will be used to assess habitat type, habitat utilization, habitat preference, and home range areas for individual animals. Diet composition for individual seals will be evaluated to determine whether specific animals are generalists or specialists. Second, the team will investigate the physiological adaptations that allow leopard seals to be apex predators and determine to what extent leopard seals are working at or near their physiological limit. Diving behavior and physiology of leopard seals will be evaluated (for instance the aerobic dive limit for individual animals and skeletal muscle adaptations will be determined for diving under hypoxic conditions). Data from time-depth recorders will be used to determine foraging strategies for individual seals, and these diving characteristics will be related to physiological variables (e.g., blood volume, muscle oxygen stores) to better understand the link between foraging behavior and physiology. The team will compare myoglobin storage in swimming muscles associated with both forelimb and hind limb propulsion and the use of anaerobic versus aerobic metabolic systems while foraging.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "MAMMALS; Stable Isotopes; Livingston Island", "locations": "Livingston Island", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Trumble, Stephen J", "platforms": null, "repo": "Dryad", "repositories": "Dryad", "science_programs": null, "south": -90.0, "title": "Collaborative Research: Foraging Ecology and Physiology of the Leopard Seal", "uid": "p0010394", "west": -180.0}, {"awards": "1645087 Catchen, Julian", "bounds_geometry": null, "dataset_titles": "Chromosome-Level Genome Assembly and Circadian Gene Repertoire of the Patagonia Blennie Eleginops maclovinus\u2014The Closest Ancestral Proxy of Antarctic Cryonotothenioids; Evaluating Illumina-, Nanopore-, and PacBio-based genome assembly strategies with the bald notothen, Trematomus borchgrevinki; Genomics of Secondarily Temperate Adaptation in the Only Non-Antarctic Icefish", "datasets": [{"dataset_uid": "200331", "doi": "10.5061/dryad.ghx3ffbs3", "keywords": null, "people": null, "repository": "Dryad", "science_program": null, "title": "Evaluating Illumina-, Nanopore-, and PacBio-based genome assembly strategies with the bald notothen, Trematomus borchgrevinki", "url": "https://datadryad.org/stash/dataset/doi:10.5061/dryad.ghx3ffbs3"}, {"dataset_uid": "200380", "doi": "", "keywords": null, "people": null, "repository": "NCBI", "science_program": null, "title": "Genomics of Secondarily Temperate Adaptation in the Only Non-Antarctic Icefish", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA857989"}, {"dataset_uid": "200381", "doi": "", "keywords": null, "people": null, "repository": "NCBI", "science_program": null, "title": "Chromosome-Level Genome Assembly and Circadian Gene Repertoire of the Patagonia Blennie Eleginops maclovinus\u2014The Closest Ancestral Proxy of Antarctic Cryonotothenioids", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA917608"}, {"dataset_uid": "200330", "doi": "", "keywords": null, "people": null, "repository": "NCBI ", "science_program": null, "title": "Evaluating Illumina-, Nanopore-, and PacBio-based genome assembly strategies with the bald notothen, Trematomus borchgrevinki", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA861284"}], "date_created": "Mon, 10 Oct 2022 00:00:00 GMT", "description": "As plate tectonics pushed Antarctica into a polar position, by ~34 million years ago, the continent and its surrounding Southern Ocean (SO) became geographically and thermally isolated by the Antarctic Circumpolar Current. Terrestrial and marine glaciation followed, resulting in extinctions as well as the survival and radiation of unique flora and fauna. The notothenioid fish survived and arose from a common ancestral stock into tax with 120 species that dominates today?s SO fish fauna. The Notothenioids evolved adaptive traits including novel antifreeze proteins for survival in extreme cold, but also suffered seemingly adverse trait loss including red blood cells in the icefish family, and the ability to mount cellular responses to mitigate heat stress ? otherwise ubiquitous across all life. This project aims to understand how the notothenoid genomes have changed and contributed to their evolution in the cold. The project will sequence, analyze and compare the genomes of two strategic pairs of notothenioid fishes representing both red-blooded and white-blooded species. Each pair will consist of one Antarctic species and one that has readapted to the temperate waters of S. America or New Zealand. The project will also compare the Antarctic species genomes to a genome of the closet non-Antarctic relative representing the temperate notothenioid ancestor. The work aims to uncover the mechanisms that enabled the adaptive evolution of this ecologically vital group of fish in the freezing Southern Ocean, and shed light on their adaptability to a warming world. The finished genomes will be made available to promote and advance Antarctic research and the project will host a symposium of Polar researchers to discuss the cutting edge developments regarding of genomic adaptations in the polar region. Despite subzero, icy conditions that are perilous to teleost fish, the fish fauna of the isolated Southern Ocean (SO) surrounding Antarctica is remarkably bountiful. A single teleost group ? the notothenioid fishes ? dominate the fauna, comprising over 120 species that arose from a common ancestor. When Antarctica became isolated and SO temperatures began to plunge in early Oligocene, the prior temperate fishes became extinct. The ancestor of Antarctic notothenioids overcame forbidding polar conditions and, absent niche competition, it diversified and filled the SO. How did notothenioids adapt to freezing environmental selection pressures and achieve such extraordinary success? And having specialized to life in chronic cold for 30 myr, can they evolve in pace with today?s warming climate to stay viable? Past studies of Antarctic notothenioid evolutionary adaptation have discovered various remarkable traits including the key, life-saving antifreeze proteins. But life specialized to cold also led to paradoxical trait changes such as the loss of the otherwise universal heat shock response, and of the O2-transporting hemoglobin and red blood cells in the icefish family. A few species interestingly regained abilities to live in temperate waters following the escape of their ancestor out of the freezing SO. This proposed project is the first major effort to advance the field from single trait studies to understanding the full spectrum of genomic and genetic responses to climatic and environmental change during notothenioid evolution, and to evaluate their adaptability to continuing climate change. To this end, the project will sequence the genomes of four key species that embody genomic responses to different thermal selection regimes during notothenioids? evolutionary history, and by comparative analyses of genomic structure, architecture and content, deduce the responding changes. Specifically, the project will (i) obtain whole genome assemblies of the red-blooded T. borchgrevinki and the S. American icefish C. esox; (ii) using the finished genomes from (i) as template, obtain assemblies of the New Zealand notothenioid N. angustata, and the white-blooded icefish C. gunnari, representing a long (11 myr) and recent (1 myr) secondarily temperate evolutionary history respectively. Genes that are under selection in the temperate environment but not in the Antarctic environment can be inferred to be directly necessary for that environment ? and the reverse is also true for genes under selection in the Antarctic but not in the temperate environment. Further, genes important for survival in temperate waters will show parallel selection between N. angustata and C. esox despite the fact that the two fish left the Antarctic at far separated time points. Finally, gene families that expanded due to strong selection within the cold Antarctic should show a degradation of duplicates in the temperate environment. The project will test these hypotheses using a number of techniques to compare the content and form of genes, the structure of the chromosomes containing those genes, and through the identification of key characters, such as selfish genetic elements, introns, and structural variants.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "Genome Assembly; FISH; McMurdo Sound; Icefish; SHIPS; Notothenioid; Puerto Natales, Chile", "locations": "McMurdo Sound; Puerto Natales, Chile", "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Catchen, Julian; Cheng, Chi-Hing", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e SHIPS", "repo": "Dryad", "repositories": "Dryad; NCBI; NCBI ", "science_programs": null, "south": null, "title": "Evolutionary Genomic Responses in Antarctic Notothenioid Fishes", "uid": "p0010384", "west": null}, {"awards": "2138994 Kocot, Kevin; 2138993 Gerken, Sarah", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": null, "datasets": null, "date_created": "Tue, 20 Sep 2022 00:00:00 GMT", "description": "Part I: General description Cumaceans are small crustaceans, commonly known as comma shrimp, that live in muddy or sandy bottom environments in marine waters. Cumaceans are important for the diet of fish, birds, and even grey whales. This research program is assessing cumacean diversity and adaptation in different regions of Antarctica and evaluate this organisms adaptations using molecular methods to a changing Antarctic region. The research stands to significantly advance understanding of invertebrate adaptations to cold, stable habitats and responses to changes in those habitats. In addition, this project is advancing understanding of the biology of Cumacea, a globally diverse and biologically important group of animals. Targeted training of early career students and professionals in cumacean biology, molecular techniques, and bioinformatics is included as part of the program. A workshop at the Los Angeles County Natural History Museum will also train 10 additional graduate students, with a focus on training for underrepresented groups. Project outreach also includes social media, outreach to schools in very diverse school districts in Anchorage, AK, and creation of museum events and an exhibit at the Alabama Museum of Natural History. Finally, engagement by the team in activities related to the National Ocean Science Bowl promotes broad engagement with high school students for Antarctic science learning. Part II: Technical Description The overarching goal of this research is to use cumaceans as a model system to explore invertebrate adaptations to the changing Antarctic. This project is leveraging integrative taxonomy, functional, comparative and evolutionary genomics, and phylogenetic comparative methods to understand the true diversity of Cumacea in the Antarctic. The team is identifying genes and gene families experiencing expansions, selection, or significant differential expression, generating a broadly sampled and robust phylogenetic framework for the Antarctic Cumacea based on transcriptomes and genomes, and exploring rates and timing of diversification. The project is providing important information related to gene gain/loss, positive selection, and differential gene expression as a function of adaptation of organisms to Antarctic habitats. Phylogenomic analyses is providing a robust phylogenetic framework for understudied Southern Ocean Cumacea. At the start of this project, only one Antarctic transcriptome was published for this organism. This project is generating sequenced genomes from 8 species, about 250 transcriptomes from about 70 species, and approximately 470 COI and 16S amplicon barcodes from about 100 species. Curated morphological reference collections will be deposited at the Smithsonian, Los Angeles County Natural History Museum and in the New Zealand National Water and Atmospheric Research collection at Greta Point to assist future researchers in identification of Antarctic cumaceans. Beyond the immediate scope of the current project, the genomic resources will be able to be leveraged by members of the polar biology and invertebrate zoology communities for diverse other uses ranging from PCR primer development to inference of ancestral population sizes. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "Benthic; SHIPS; Antarctic Peninsula; Antarctica; Biodiversity; Peracarida; ARTHROPODS; East Antarctica; Chile; BENTHIC; Cumacea; Ross Sea; Crustacea", "locations": "Antarctica; East Antarctica; Chile; Ross Sea; Antarctic Peninsula", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Polar Special Initiatives; Antarctic Organisms and Ecosystems", "paleo_time": "NOT APPLICABLE", "persons": "Gerken, Sarah; Kocot, Kevin", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e SHIPS", "repositories": null, "science_programs": null, "south": -90.0, "title": "Collaborative Research: ANT LIA: Cumacean -Omics to Measure Mode of Adaptation to Antarctica (COMMAA)", "uid": "p0010379", "west": -180.0}, {"awards": "2147045 Learman, Deric", "bounds_geometry": "POLYGON((-180 -60,-168 -60,-156 -60,-144 -60,-132 -60,-120 -60,-108 -60,-96 -60,-84 -60,-72 -60,-60 -60,-60 -62,-60 -64,-60 -66,-60 -68,-60 -70,-60 -72,-60 -74,-60 -76,-60 -78,-60 -80,-72 -80,-84 -80,-96 -80,-108 -80,-120 -80,-132 -80,-144 -80,-156 -80,-168 -80,180 -80,171 -80,162 -80,153 -80,144 -80,135 -80,126 -80,117 -80,108 -80,99 -80,90 -80,90 -78,90 -76,90 -74,90 -72,90 -70,90 -68,90 -66,90 -64,90 -62,90 -60,99 -60,108 -60,117 -60,126 -60,135 -60,144 -60,153 -60,162 -60,171 -60,-180 -60))", "dataset_titles": "Physical and geochemical data from shelf sediments eastern Antarctica", "datasets": [{"dataset_uid": "601876", "doi": "10.15784/601876", "keywords": "Antarctica; Cryosphere", "people": "Learman, Deric", "repository": "USAP-DC", "science_program": null, "title": "Physical and geochemical data from shelf sediments eastern Antarctica", "url": "https://www.usap-dc.org/view/dataset/601876"}], "date_created": "Tue, 30 Aug 2022 00:00:00 GMT", "description": "Microbes in Antarctic surface marine sediments have an important role in degrading organic matter and releasing nutrients to the ocean. Organic matter degradation is at the center of the carbon cycle in the ocean, providing valuable information on nutrient recycling, food availability to animals and carbon dioxide release to the atmosphere. The functionality of these microbes has been inferred by their genomics, however these methods only address the possible function, not their actual rates. In this project the PIs plan to combine genomics methods with cellular estimates of enzyme abundance and activity as a way to determine the rates of carbon degradation. This project aims to sample in several regions of Antarctica to provide a large-scale picture of the processes under study and understand the importance of microbial community composition and environmental factors, such as primary productivity, have on microbial activity. The proposed work will combine research tools such as metagenomics, meta-transcriptomics, and metabolomics coupled with chemical data and enzyme assays to establish degradation of organic matter in Antarctic sediments. This project benefits NSFs goals of understanding the adaptation of Antarctic organisms to the cold and isolated environment, critical to predict effects of climate change to polar organisms, as well as contribute to our knowledge of how Antarctic organisms have adapted to this environment. Society will benefit from this project by education of 2 graduate students, undergraduates and K-12 students as well as increase public literacy through short videos production shared in YouTube. The PIs propose to advance understanding of polar microbial community function, by measuring enzyme and gene function of complex organic matter degradation in several ocean regions, providing a circum-Antarctic description of sediment processes. Two hypotheses are proposed. The first hypothesis states that many genes for the degradation of complex organic matter will be shared in sediments throughout a sampling transect and that where variations in gene content occur, it will reflect differences in the quantity and quality of organic matter, not regional variability. The second hypothesis states that a fraction of gene transcripts for organic matter degradation will not result in measurable enzyme activity due to post-translational modification or rapid degradation of the enzymes. The PIs will analyze sediment cores already collected in a 2020 cruise to the western Antarctic Peninsula with the additional request of participating in a cruise in 2023 to East Antarctica. The PIs will analyze sediments for metagenomics, meta-transcriptomics, and metabolomics coupled with geochemical data and enzyme assays to establish microbial degradation of complex organic matter in Antarctic sediments. Organic carbon concentrations and content in sediments will be measured with \u03b413C, \u03b415N, TOC porewater fluorescence in bulk organic carbon. Combined with determination of geographical variability as well as dependence on carbon sources, results from this study could provide the basis for new hypotheses on how climate variability, with increased water temperature, affects geochemistry in the Southern Ocean. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 90.0, "geometry": "POINT(-165 -70)", "instruments": null, "is_usap_dc": true, "keywords": "BENTHIC; ECOSYSTEM FUNCTIONS; Weddell Sea; Antarctic Peninsula; SEDIMENT CHEMISTRY; R/V NBP", "locations": "Antarctic Peninsula; Weddell Sea", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Learman, Deric", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -80.0, "title": "Collaborative Research: ANT LIA: Connecting Metagenome Potential to Microbial Function: Investigating Microbial Degradation of Complex Organic Matter Antarctic Benthic Sediments", "uid": "p0010373", "west": -60.0}, {"awards": "1443637 Zakon, Harold", "bounds_geometry": null, "dataset_titles": "Evolutionary analysis of transient receptor potential (TRP) channels in notothenioid fishes; Functional characterization of temperature activated ion channels from Antarctic fishes; TagSeq tissue specific expression data for Antarctic Harpagifer antarcticus and tropical African cichlid Astatotilapia (Haplochromis) burtoni", "datasets": [{"dataset_uid": "200292", "doi": "10.18738/T8/NXGNEI", "keywords": null, "people": null, "repository": "Texas Data Repository", "science_program": null, "title": "Evolutionary analysis of transient receptor potential (TRP) channels in notothenioid fishes", "url": "https://doi.org/10.18738/T8/NXGNEI"}, {"dataset_uid": "200293", "doi": "", "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "TagSeq tissue specific expression data for Antarctic Harpagifer antarcticus and tropical African cichlid Astatotilapia (Haplochromis) burtoni", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA758918"}, {"dataset_uid": "601695", "doi": "10.15784/601695", "keywords": "Antarctica; Notothenioid; Southern Ocean", "people": "York, Julia", "repository": "USAP-DC", "science_program": null, "title": "Functional characterization of temperature activated ion channels from Antarctic fishes", "url": "https://www.usap-dc.org/view/dataset/601695"}], "date_created": "Fri, 03 Jun 2022 00:00:00 GMT", "description": "This project studies how the proteins of the nerves and muscles of fish that live in Antarctica function in the cold, which should provide information on the function of these same proteins in all animals, including humans. These proteins, called ion channels, open and close to allow ions (atoms or molecules with electrical charge) to flow into or out of cells which causes the electrical activity of nerves and muscles. Mutations that influence this process are the basis of numerous human disorders such as epilepsy, heart arrhythmias, and muscle paralysis. Thus, it is important to understand what parts of the proteins govern these transitions. The speed with which channels open and close depends on temperature. Human ion channels transition slowly when we are cold, which is why we become numb in the cold. Yet Antarctic fish, called icefish, are active at freezing temperatures that drastically limit the activity of human ion channels. The investigators have evidence that specific alterations in the icefishs\u0027 ion channels allow their channels to operate differently in the cold and they will use gene discovery and biophysical methods to test how these changes alter the transitions of icefish proteins at different temperatures. The project will also further the NSF goals of training new generations of scientists and of making scientific discoveries available to the general public. The gene discovery analysis will be done by undergraduate students including those from a minority-serving university and the investigators will develop a new course which will also serve students at that institution and elsewhere. In addition, the investigators will participate in educational outreach events with the general public as well as with groups with special needs. Notothenioid fishes are one of the most successful groups of vertebrates in Antarctica. Notothens have adaptations to the freezing water they inhabit and this project will study how their voltage-gated ion channels (VGICs) function in the cold. The molecular movements of ion channels are severely impaired by cold, yet notothens function at temperatures that would paralyze the nerves and muscles of \"cold-blooded\" temperate zone animals. Surprisingly, no biophysical or molecular investigations have been conducted on notothen VGICs. The investigators have preliminary data that amino acid substitutions occur at sites in VGICs that are evolutionarily conserved from fruit flies to humans. Some of these sites are known to impact channel function and the role of others in channel transitioning are unknown. The results from studying them will provide novel information also applicable to non-notothen, perhaps even human, VGICs as well as providing insights into how VGICs adapt to the cold. The project will biophysically characterize notothen VGICs using voltage-clamp techniques will and compare their properties over a range of temperatures to the same channel from two temperate zone fish. The role of unique notothen amino acid substitutions will be characterized by mutagenesis. One specific aim will be a project in which undergraduates mine notothen sequence databases to identify other potential amino acid substitutions in VGICs that might facilitate adaptation to the cold.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "Amd/Us; USAP-DC; USA/NSF; FIELD INVESTIGATION; AMD; FISHERIES", "locations": null, "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Zakon, Harold", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION", "repo": "Texas Data Repository", "repositories": "GenBank; Texas Data Repository; USAP-DC", "science_programs": null, "south": null, "title": "Analysis of Voltage-gated Ion Channels in Antarctic Fish", "uid": "p0010331", "west": null}, {"awards": "1951090 Stukel, Michael", "bounds_geometry": "POLYGON((-80 -63,-78.2 -63,-76.4 -63,-74.6 -63,-72.8 -63,-71 -63,-69.2 -63,-67.4 -63,-65.6 -63,-63.8 -63,-62 -63,-62 -63.7,-62 -64.4,-62 -65.1,-62 -65.8,-62 -66.5,-62 -67.2,-62 -67.9,-62 -68.6,-62 -69.3,-62 -70,-63.8 -70,-65.6 -70,-67.4 -70,-69.2 -70,-71 -70,-72.8 -70,-74.6 -70,-76.4 -70,-78.2 -70,-80 -70,-80 -69.3,-80 -68.6,-80 -67.9,-80 -67.2,-80 -66.5,-80 -65.8,-80 -65.1,-80 -64.4,-80 -63.7,-80 -63))", "dataset_titles": "BCO-DMO Project Page", "datasets": [{"dataset_uid": "200294", "doi": null, "keywords": null, "people": null, "repository": "BCO-DMO", "science_program": null, "title": "BCO-DMO Project Page", "url": "https://www.bco-dmo.org/project/838048"}], "date_created": "Fri, 03 Jun 2022 00:00:00 GMT", "description": "Algae in the surface ocean convert carbon dioxide into organic carbon through photosynthesis. The biological carbon pump transports this organic carbon from the atmosphere to the deep ocean where it can be stored for tens to hundreds of years. Annually, the amount transported is similar to that humans are currently emitting by burning fossil fuels. However, at present we cannot predict how this important process will change with a warming ocean. These investigators plan to develop a 15+ year time-series of vertical carbon transfer for the Western Antarctic Peninsula; a highly productive Antarctic ecosystem. This region is also rapid transition to warmer temperatures leading to reduced sea ice coverage. This work will help researchers better understand how the carbon cycle in the Western Antarctic Peninsula will respond to climate change. The researchers will develop the first large-scale time-series of carbon flux anywhere in the ocean. This research will also support the education and training of a graduate student and support the integration of concepts in Antarctic research into two undergraduate courses designed for non-science majors and advanced earth science students. The researchers will also develop educational modules for introducing elementary and middle-school age students to important concepts such as gross and net primary productivity, feedbacks in the marine and atmospheric systems, and the differences between correlation and causation. Results from this proposal will also be incorporated into a children\u2019s book, \u201cPlankton do the Strangest Things\u201d, that is targeted at 5-7 year olds and is designed to introduce them to the incredible diversity and fascinating adaptations of microscopic marine organisms. This research seeks to leverage 6 years (2015-2020) of 234Th samples collected on Palmer LTER program, 5 years of prior measurements (2009-2010, 2012-2014), and upcoming cruises (2021-2023) to develop a time-series of summertime particle flux in the WAP that stretches for 15 years. The 238U-234Th disequilibrium approach utilizes changes in the activity of the particle-active radio-isotope 234Th relative to its parent nuclide 238U to quantify the flux of sinking carbon out of the surface ocean (over a time-scale of ~one month). This proposal will fund 234Th analyses from nine years\u2019 worth of cruises (2015-2023) and extensive analyses designed to investigate the processes driving inter-annual variability in the BCP. These include: 1) physical modeling to quantify the importance of advection and diffusion in the 234Th budget, 2) time-series analyses of particle flux, and 3) statistical modeling of the relationships between particle flux and multiple presumed drivers (biological, chemical, physical, and climate indices) measured by collaborators in the Palmer LTER program. This multi-faceted approach is critical for linking the measurements to models and for predicting responses to climate change. It will also test the hypothesis that export flux is decreasing in the northern WAP, increasing in the southern WAP, and increasing when integrated over the entire region as a result of earlier sea ice retreat and a larger ice-free zone. The project will also investigate relationships between carbon export and multiple potentially controlling factors including: primary productivity, algal biomass and taxonomic composition, biological oxygen saturation, zooplankton biomass and taxonomic composition, bacterial production, temperature, wintertime sea ice extent, date of sea ice retreat, and climate modes. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": -62.0, "geometry": "POINT(-71 -66.5)", "instruments": null, "is_usap_dc": true, "keywords": "Amd/Us; AMD; FIELD INVESTIGATION; Palmer Station; USAP-DC; BIOGEOCHEMICAL CYCLES; USA/NSF", "locations": "Palmer Station", "north": -63.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Stukel, Michael", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION", "repo": "BCO-DMO", "repositories": "BCO-DMO", "science_programs": null, "south": -70.0, "title": "Quantifying Processes Driving Interannual Variability in the Biological Carbon Pump in the Western Antarctic Peninsula", "uid": "p0010332", "west": -80.0}, {"awards": "1951500 Jenouvrier, Stephanie", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "Data from: Individual life histories: Neither slow nor fast, just diverse; Evo-Demo Hyperstate Matrix Model Code Repository; Hyperstate matrix model reveals the influence of personality on demography; Individual life histories: neither slow nor fast, just diverse; Plastic Behaviour Buffers Climate Variability in the Wandering Albatross; Strong winds reduce foraging success in albatrosses; Subtropical anticyclone impacts life-history traits of a marine top predator; The impact of boldness on demographic rates and lifehistory outcomes in the wandering albatross", "datasets": [{"dataset_uid": "200454", "doi": "", "keywords": null, "people": null, "repository": "GITHUB", "science_program": null, "title": "Evo-Demo Hyperstate Matrix Model Code Repository", "url": "https://github.com/fledge-whoi/Eco-EvoHyperstateModel"}, {"dataset_uid": "200459", "doi": "https://doi.org/10.5281/zenodo.13881532", "keywords": null, "people": null, "repository": "ZENODO", "science_program": null, "title": "Strong winds reduce foraging success in albatrosses", "url": "https://zenodo.org/records/13881532"}, {"dataset_uid": "200457", "doi": " https://zenodo.org/doi/10.5281/zenodo.10887354", "keywords": null, "people": null, "repository": "ZENODO", "science_program": null, "title": "Plastic Behaviour Buffers Climate Variability in the Wandering Albatross", "url": "https://zenodo.org/records/14290546"}, {"dataset_uid": "200455", "doi": "", "keywords": null, "people": null, "repository": "GITHUB", "science_program": null, "title": "Hyperstate matrix model reveals the influence of personality on demography", "url": "https://github.com/fledge-whoi/HyperstateWApopulationmodel"}, {"dataset_uid": "200453", "doi": "10.5061/dryad.3bk3j9kpm", "keywords": null, "people": null, "repository": "Dryad", "science_program": null, "title": "Data from: Individual life histories: Neither slow nor fast, just diverse", "url": "https://doi.org/10.5061/dryad.3bk3j9kpm"}, {"dataset_uid": "601770", "doi": "10.15784/601770", "keywords": "Antarctica; Cryosphere; Demography; Sub-Antarctic", "people": "Joanie, Van de Walle; Jenouvrier, Stephanie", "repository": "USAP-DC", "science_program": null, "title": "The impact of boldness on demographic rates and lifehistory outcomes in the wandering albatross", "url": "https://www.usap-dc.org/view/dataset/601770"}, {"dataset_uid": "200458", "doi": "https://doi.org/10.5061/dryad.3bk3j9kpm", "keywords": null, "people": null, "repository": "DRYAD", "science_program": null, "title": "Individual life histories: neither slow nor fast, just diverse", "url": "https://doi.org/10.6084/m9.figshare.c.6181063."}, {"dataset_uid": "200456", "doi": "", "keywords": null, "people": null, "repository": "GITHUB", "science_program": null, "title": "Subtropical anticyclone impacts life-history traits of a marine top predator", "url": "https://github.com/fledge-whoi/Alba_Mascarene-High"}], "date_created": "Wed, 08 Dec 2021 00:00:00 GMT", "description": "Part I: Nontechnical description: This award represents a collaborative geoscience research effort between US NSF and UK Natural Environment Research Council (NERC) researchers with efforts in each nation funded by their respective countries (Dear Colleague Letter NSF 16-132). The research will focus on understanding the links between behavior, ecology, and evolution in a Southern Ocean wandering albatross population in response to global changes in climate and in exploitation of natural resources. The most immediate response of animals to global change typically is behavioral, and this work will provide a more comprehensive understanding of how differences individual bird behavior affect evolution and adaptation for the population under changing environments. Characterization of albatross personality, life-history traits, and population dynamics collected over long time scales will be used to develop robust forecasting of species persistence in the face of future global changes. The results of this project will feed into conservation and management decisions for endangered Southern Ocean species. The work will also be used to provide specific research training at all levels, including a postdoctoral scholar, graduate students and K-12 students. It will also support education for the public about impacts from human-induced activities on our polar ecosystems using animations, public lectures, printed and web media. Part II: Technical description Past research has shown that individual animal personalities range over a continuum of behavior, such that some individuals are consistently more aggressive, more explorative, and bolder than others. How the phenotypic distributions of personality and foraging behavior types within a population is created and maintained by ecological (demographic and phenotypic plasticity) and evolutionary (heritability) processes remain an open question. Differences in personality traits determine how individuals acquire resources and how they allocate these to reproduction and survival. Although some studies have found different foraging behaviors or breeding performances between personality types, none have established the link between personality differences in foraging behaviors and life histories (both reproduction and survival, and their covariations) in the context of global change. Furthermore, plasticity in foraging behaviors is not considered in the pace-of-life syndrome, which has potentially hampered our ability to find covariation between personality and life history trade-off. This project will fill these knowledge gaps and develop an eco-evolutionary model of the complex interactions among individual personality and foraging plasticity, heritability of personality and foraging behaviors, life history strategies, population dynamics in a changing environment (fisheries and climate) using a long-term database consisting of ~1,800 tagged wandering albatross seabirds (Diomedea exulans) with defined individual personalities and life history traits breeding in the Southern Ocean. Climate projections from IPCC atmospheric-oceanic global circulation models will be used to provide projections of population structure under future global change conditions. Specifically, the team will (1) characterize the differences in life history strategies along the shy-bold continuum of personalities and across environmental conditions; (2) develop the link between phenotypic plasticity in foraging effort and personality; (3) characterize the heritability of personality and foraging behaviors; (4) develop a stochastic eco-evolutionary model to predict population growth rates in a changing environment. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "USAP-DC; AMD; ECOLOGICAL DYNAMICS; OCEAN TEMPERATURE; USA/NSF; Antarctica; FIELD INVESTIGATION; SPECIES/POPULATION INTERACTIONS; PENGUINS; Amd/Us", "locations": "Antarctica", "north": -60.0, "nsf_funding_programs": "Antarctic Integrated System Science; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Jenouvrier, Stephanie; Patrick, Samantha", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION", "repo": "GITHUB", "repositories": "Dryad; DRYAD; GITHUB; USAP-DC; ZENODO", "science_programs": null, "south": -90.0, "title": "NSFGEO-NERC: Integrating Individual Personality Differences in the Evolutionary Ecology of a Seabird in the Rapidly Changing Polar Environment", "uid": "p0010283", "west": -180.0}, {"awards": "1744999 Todgham, Anne", "bounds_geometry": "POLYGON((162 -77,162.8 -77,163.6 -77,164.4 -77,165.2 -77,166 -77,166.8 -77,167.6 -77,168.4 -77,169.2 -77,170 -77,170 -77.1,170 -77.2,170 -77.3,170 -77.4,170 -77.5,170 -77.6,170 -77.7,170 -77.8,170 -77.9,170 -78,169.2 -78,168.4 -78,167.6 -78,166.8 -78,166 -78,165.2 -78,164.4 -78,163.6 -78,162.8 -78,162 -78,162 -77.9,162 -77.8,162 -77.7,162 -77.6,162 -77.5,162 -77.4,162 -77.3,162 -77.2,162 -77.1,162 -77))", "dataset_titles": "A comparative and ontogenetic examination of mitochondrial function in Antarctic notothenioid species; Differential temperature preferences exhibited in the juvenile Antarctic notothenioids Trematomus bernacchii and Trematomus pennellii", "datasets": [{"dataset_uid": "601766", "doi": null, "keywords": "Antarctica; McMurdo Sound", "people": "Naslund, Andrew; Frazier, Amanda; Todgham, Anne; Mandic, Milica", "repository": "USAP-DC", "science_program": null, "title": "A comparative and ontogenetic examination of mitochondrial function in Antarctic notothenioid species", "url": "https://www.usap-dc.org/view/dataset/601766"}, {"dataset_uid": "601765", "doi": null, "keywords": "Antarctica; McMurdo Sound; Ross Sea", "people": "Todgham, Anne; Frazier, Amanda; Mandic, Milica; Zillig, Ken; Naslund, Andrew", "repository": "USAP-DC", "science_program": null, "title": "Differential temperature preferences exhibited in the juvenile Antarctic notothenioids Trematomus bernacchii and Trematomus pennellii", "url": "https://www.usap-dc.org/view/dataset/601765"}], "date_created": "Thu, 12 Aug 2021 00:00:00 GMT", "description": "The Southern Ocean contains an extraordinary diversity of marine life. Many Antarctic marine organisms have evolved in stable, cold ocean conditions and possess limited ability to respond to environmental fluctuations. To date, research on the physiological limits of Antarctic fishes has focused largely on adult life stages. However, early life stages may be more sensitive to environmental change because they may need to prioritize energy to growth and development instead of maintenance of physiological balance and integrity- even under stress conditions. This project will examine the specific mechanisms that young (embryos, larvae and juveniles) Antarctic fishes use to respond to changes in ocean conditions at the molecular, cellular and physiological levels, so that they are able to survive. The aim is to provide a unifying framework for linking environmental change, gene expression, metabolism and organismal performance in different species that have various rates of growth and development. There is a diverse and robust education and outreach program linked with the research effort that will reach students, teachers, young scientists, community members and government officials at local and regions scales. Polar species have already been identified as highly vulnerable to global change. However as yet, there is no unifying framework for linking environmental change to organismal performance, in part because a mechanistic understanding of how stressors interact at the molecular, biochemical and physiological level is underdeveloped is lacking for most species. In the marine environment, this paucity of information limits our capacity to accurately predict the impacts of warming and CO2-acidification on polar species, and therefore prevents linking climate model projections to population health predictions. This research will evaluate whether metabolic capacity (i.e. the ability to match energy supply with energy demand) limits the capacity of Antarctic fishes to acclimate to the simultaneous stressors of ocean warming and CO2-acidification. If species are unable to reestablish metabolic homeostasis following exposure to stressors, increased energetic costs may lead to a decline in physiological performance, organismal fitness, and survival. This energy-mismatch hypothesis will be tested in a multi-species approach that focuses on the early life stages, as growing juveniles are likely more vulnerable to energetic constraints than adults, while different species are targeted in order to understand how differences in phenology and life history traits influence metabolic plasticity. The research will provide a mechanistic integration of gene expression and metabolite patterns, and metabolic responses at the cellular and whole organism levels to broadly understand metabolic plasticity of fishes. The research is aligned with the theme \"Decoding the genomic and transcriptomic bases of biological adaptation and response across Antarctic organisms and ecosystems\" which is one of three major themes identified by the National Academy of Sciences in their document \"A Strategic Vision for NSF Investments in Antarctic and Southern Ocean Research\". Additionally, this project builds environmental stewardship and awareness by increasing science literacy in the broader community in three main ways: First it will increase the diversity of students involved in environmental science research by supporting one PhD student, one postdoctoral scholar and two undergraduate students and promoting the training of young students from groups traditionally underrepresented in environmental biology. Second, the project will participate in UC Davis\u0027s OneClimate initiative, which leverages the community\u0027s expertise to develop broad perspectives regarding climate change, science and society, and engage K-12 students, government officials, and local and statewide communities on topics of Antarctic research, organismal adaptation as well as ongoing and potential future changes at the poles. Lastly, summer workshops will be conducted in collaborations with the NSF-funded education program APPLES (Arctic Plant Phenology: Learning through Engaged Science), to engage teachers and K-12 students in polar science. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 170.0, "geometry": "POINT(166 -77.5)", "instruments": null, "is_usap_dc": true, "keywords": "FIELD SURVEYS; AMD; McMurdo Sound; FISH; USA/NSF; Amd/Us; USAP-DC", "locations": "McMurdo Sound", "north": -77.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Todgham, Anne", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -78.0, "title": "Interacting Stressors: Metabolic Capacity to Acclimate under Ocean Warming and CO2- Acidification in Early Developmental Stages of Antarctic Fishes", "uid": "p0010241", "west": 162.0}, {"awards": "1927709 Friedlaender, Ari; 1947453 Hunt, Kathleen; 1927742 Fleming, Alyson", "bounds_geometry": "POLYGON((150 -60,153 -60,156 -60,159 -60,162 -60,165 -60,168 -60,171 -60,174 -60,177 -60,180 -60,180 -61.5,180 -63,180 -64.5,180 -66,180 -67.5,180 -69,180 -70.5,180 -72,180 -73.5,180 -75,177 -75,174 -75,171 -75,168 -75,165 -75,162 -75,159 -75,156 -75,153 -75,150 -75,150 -73.5,150 -72,150 -70.5,150 -69,150 -67.5,150 -66,150 -64.5,150 -63,150 -61.5,150 -60))", "dataset_titles": "Bulk stable isotope data of blue and fin whales; Hormone meta data for Antarctic blue and fin whales", "datasets": [{"dataset_uid": "601908", "doi": "10.15784/601908", "keywords": "Antarctica; Biota; Blue Whale; Cryosphere; Fin Whale; Hormones; Oceans; Reproduction; Whales", "people": "Hunt, Kathleen; Fleming, Alyson", "repository": "USAP-DC", "science_program": null, "title": "Hormone meta data for Antarctic blue and fin whales", "url": "https://www.usap-dc.org/view/dataset/601908"}, {"dataset_uid": "601901", "doi": "10.15784/601901", "keywords": "Antarctica; Biota; Cryosphere; Isotope; Southern Ocean; Whales", "people": "Smith, Malia; Fleming, Alyson", "repository": "USAP-DC", "science_program": null, "title": "Bulk stable isotope data of blue and fin whales", "url": "https://www.usap-dc.org/view/dataset/601901"}], "date_created": "Tue, 10 Aug 2021 00:00:00 GMT", "description": "Blue and fin whales are the two largest animals on the planet, and the two largest krill predators in the Southern Ocean. Commercial whaling in Antarctic waters started in the early 1900?s, and by the 1970\u0027s whale populations were reduced from thousands to only a few hundred individuals. The absence of data about whale biology and ecology prior to these large population reductions has limited our understanding of how the ecosystem functioned when cetacean populations were more robust. However, an archive of baleen plates from 800 Antarctic blue and fin whales harvested between 1946 and 1948 was recently rediscovered in the Smithsonian\u0027s National Museum of Natural History that will shed insight into historic whale ecology. As baleen grows, it incorporates circulating hormones, and compounds from the whale\u0027s diet, recording continuous biological and oceanographic information across multiple years. This project will apply a suite of modern molecular techniques to these archived specimens to ask how blue and fin whale foraging and reproduction responded to climate variability, changes at the base of the food web, and whaling activities in the early 1940s. By comparison with more modern datasets, these investigations will fill major gaps in understanding of the largest krill predators, their response to disturbance and environmental change, and the impact that commercial whaling has had on the structure and function of the Antarctic marine ecosystem. This project will improve stem education through annual programming for middle and high school girls in partnership with UNCW\u0027s Marine Quest program. Public outreach will occur through partnerships with the Smithsonian and the International Association of Antarctic Tour Operators to deliver emerging research on Antarctic ecosystems and highlight the contemporary relevance and scientific value of museum collections. Examination of past conditions and adaptations of polar biota is fundamental to predictions of future climate change scenarios. The baleen record that will be used in this study forms an ideal experimental platform for studying bottom-up, top-down and anthropogenic impacts on blue and fin whales. This historic baleen archive includes years with strong climate and temperature anomalies allowing the influence of climate variability on predators and the ecosystems that support them to be examined. Additionally, the impact of commercial whaling on whale stress levels will be investigated by comparing years of intensive whaling with the non-whaling years of WWII, both of which are captured in the time series. There are three main approaches to this project. First, bulk stable isotope analysis will be used to examine the trophic dynamics of Antarctic blue and fin whales. Second, compound-specific stable isotope analyses (CSIA-AA) will characterize the biogeochemistry of the base of the Antarctic food web. Finally, analyses of hormone levels in baleen will reveal differences in stress levels and reproductive status of individuals, and inform understanding of cetacean population biology. This project will generate a new public data archive to foster research opportunities across various components of the OPP program, all free from the logistical constraints of Antarctic field work. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 180.0, "geometry": "POINT(165 -67.5)", "instruments": null, "is_usap_dc": true, "keywords": "ECOSYSTEM FUNCTIONS; PELAGIC; MAMMALS; LABORATORY; AMD; Amd/Us; Southern Ocean; USAP-DC; USA/NSF", "locations": "Southern Ocean", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Fleming, Alyson; Friedlaender, Ari; McCarthy, Matthew; Hunt, Kathleen", "platforms": "OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -75.0, "title": "Collaborative Research: A New Baseline for Antarctic Blue and Fin Whales", "uid": "p0010240", "west": 150.0}, {"awards": "1644155 Twining, Benjamin", "bounds_geometry": "POLYGON((78 -68.4,78.05 -68.4,78.1 -68.4,78.15 -68.4,78.2 -68.4,78.25 -68.4,78.3 -68.4,78.35 -68.4,78.4 -68.4,78.45 -68.4,78.5 -68.4,78.5 -68.419,78.5 -68.438,78.5 -68.457,78.5 -68.476,78.5 -68.495,78.5 -68.514,78.5 -68.533,78.5 -68.552,78.5 -68.571,78.5 -68.59,78.45 -68.59,78.4 -68.59,78.35 -68.59,78.3 -68.59,78.25 -68.59,78.2 -68.59,78.15 -68.59,78.1 -68.59,78.05 -68.59,78 -68.59,78 -68.571,78 -68.552,78 -68.533,78 -68.514,78 -68.495,78 -68.476,78 -68.457,78 -68.438,78 -68.419,78 -68.4))", "dataset_titles": "Flow cytometry enumeration of virus-like and bacteria-like abundance in Ace, Deep, \u0026 Organic lakes (Antarctica)", "datasets": [{"dataset_uid": "601626", "doi": "10.15784/601626", "keywords": "Ace Lake; Antarctica; Deep Lake; Organic Lake; Vestfold Hills", "people": "Martinez-Martinez, Joaquin; Twining, Benjamin", "repository": "USAP-DC", "science_program": null, "title": "Flow cytometry enumeration of virus-like and bacteria-like abundance in Ace, Deep, \u0026 Organic lakes (Antarctica)", "url": "https://www.usap-dc.org/view/dataset/601626"}], "date_created": "Fri, 06 Aug 2021 00:00:00 GMT", "description": "Viruses are prevalent in aquatic environments where they reach up to five hundred million virus particles in a teaspoon of water. Ongoing discovery of viruses seems to confirm current understanding that all forms of life can host and be infected by viruses and that viruses are one of the largest reservoirs of unexplored genetic diversity on Earth. This study aims to better understand interactions between specific viruses and phytoplankton hosts and determine how these viruses may affect different algal groups present within lakes of the Vestfold Hills, Antarctica. These lakes (Ace, Organic and Deep)were originally derived from the ocean and contain a broad range of saline conditions with a similarly broad range of physicochemical characteristics resulting from isolation and low external influence for thousands of years. These natural laboratories allow examination of microbial processes and interactions that would be difficult to characterize elsewhere on earth. The project will generate extensive genomic information that will be made freely available. The project will also leverage the study of viruses and the genomic approaches employed to advance the training of undergraduate students and to engage and foster an understanding of Antarctic science and studies of microbes during a structured informal education program in Maine for the benefit of high school students. By establishing the dynamics and interactions of (primarily) specific dsDNA virus groups in different habitats with different redox conditions throughout seasonal and inter annual cycles the project will learn about the biotic and abiotic factors that influence microbial community dynamics. This project does not require fieldwork in Antarctica. Instead, the investigators will leverage already collected and archived samples from three lakes that have concurrent measures of physicochemical information. Approximately 2 terabyte of Next Generation Sequencing (NGS) (including metagenomes, SSU rRNA amplicons and single virus genomes) will be generated from selected available samples through a Community Science Program (CSP) funded by the Joint Genome Institute. The investigators will employ bioinformatics to interrogate those sequence databases. In particular, they will focus on investigating the presence, phylogeny and co-occurrence of polintons, polinton-like viruses, virophages and large dsDNA phytoplankton viruses as well as of their putative eukaryotic microbial hosts. Bioinformatic analyses will be complemented with quantitative digital PCR and microbial association network analysis to detect specific virus?virus?host interactions from co-occurrence spatial and temporal patterns. Multivariate analysis and network analyses will also be performed to investigate which abiotic factors most closely correlate with phytoplankton and virus abundances, temporal dynamics, and observed virus-phytoplankton associations within the three lakes. The results of this project will improve understanding of phytoplankton and their viruses as vital components of the carbon cycle in Antarctic, marine-derived aquatic environments, and likely in any other aquatic environment. Overall, this work will advance understanding of the genetic underpinnings of adaptations in unique Antarctic environments.", "east": 78.5, "geometry": "POINT(78.25 -68.495)", "instruments": null, "is_usap_dc": true, "keywords": "LABORATORY; AMD; USAP-DC; VIRUSES; Vestfold Hills; Amd/Us; FIELD SURVEYS; USA/NSF", "locations": "Vestfold Hills", "north": -68.4, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Twining, Benjamin; Martinez-Martinez, Joaquin", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS; OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -68.59, "title": "Viral control of microbial communities in Antarctic lakes", "uid": "p0010237", "west": 78.0}, {"awards": "1746087 Tarrant, Ann", "bounds_geometry": "POLYGON((-80 -60,-77.5 -60,-75 -60,-72.5 -60,-70 -60,-67.5 -60,-65 -60,-62.5 -60,-60 -60,-57.5 -60,-55 -60,-55 -61,-55 -62,-55 -63,-55 -64,-55 -65,-55 -66,-55 -67,-55 -68,-55 -69,-55 -70,-57.5 -70,-60 -70,-62.5 -70,-65 -70,-67.5 -70,-70 -70,-72.5 -70,-75 -70,-77.5 -70,-80 -70,-80 -69,-80 -68,-80 -67,-80 -66,-80 -65,-80 -64,-80 -63,-80 -62,-80 -61,-80 -60))", "dataset_titles": "Calanoides acutus: Transcriptome and gene expression data; BioProject PRJNA757455; Calanus propinquus: Transcriptome and gene expression data; BioProject PRJNA669816; Expedition data of LMG1901; Rhincalanus gigas: Transcriptome and gene expression data; BioProject PRJNA666170", "datasets": [{"dataset_uid": "200283", "doi": "", "keywords": null, "people": null, "repository": "NCBI", "science_program": null, "title": "Calanus propinquus: Transcriptome and gene expression data; BioProject PRJNA669816", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA669816"}, {"dataset_uid": "200239", "doi": "", "keywords": null, "people": null, "repository": "NCBI", "science_program": null, "title": "Rhincalanus gigas: Transcriptome and gene expression data; BioProject PRJNA666170", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA666170"}, {"dataset_uid": "200125", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG1901", "url": "https://www.rvdata.us/search/cruise/LMG1901"}, {"dataset_uid": "200284", "doi": "", "keywords": null, "people": null, "repository": "NCBI", "science_program": null, "title": "Calanoides acutus: Transcriptome and gene expression data; BioProject PRJNA757455", "url": "https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA757455"}], "date_created": "Fri, 06 Aug 2021 00:00:00 GMT", "description": "Animals in the polar oceans have adapted to dramatic seasonal changes in day length, food availability, and ice cover, as well as to consistently cold waters. This project focuses on the adaptations of copepods - small animals that live in the water column and are an important food source to many different predators. The field studies will take place in the western Antarctic Peninsula, an environment and ecosystem that is rapidly changing. Antarctic copepods have developed particular feeding and behavioral strategies to survive in their very seasonal environment, however it is not known how each of these species will respond to environmental change. The overall goal of this project is to examine and compare these adaptations across species and to understand how each species responds to short-term changes in food availability. The project contains three main objectives: the first objective is to compare the sets of genes across species, especially looking at genes related to storage of energy from food. The second objective is to measure and compare the responses of copepods to changes in food availability. The third objective is to determine how variation across the western Antarctic Pensinsula habitat affects the feeding condition of the copepods. To make the data more useful to the broader research community, a database will be developed enabling easy comparison of genetic information between copepod species. This project will provide hands-on training opportunities to graduate and undergraduate student and will seek to recruit students from underrepresented groups. Results and scientific concepts will be shared through outreach activities, including an expedition blog, a series of interactive animations, and public presentations. Polar marine organisms have adapted to dramatic seasonal changes in photoperiod, light intensity, and ice cover, as well as to cold but stable thermal environments. The western Antarctic Peninsula, the focal region for the field studies, has experienced rapid warming and ice melt. While it is difficult to predict exactly how physical conditions in this region will change, effects on species distributions have already been documented. Large Antarctic copepods in the families Calanidae and Rhincalanidae are dominant components of the mesozooplankton that use different metabolic and behavioral strategies to optimize their use of a highly seasonal food supply. The overall goal of this project is to leverage molecular approaches to examine the physiological and metabolic adaptations at the individual and species level. The project focuses on three main objectives: the first objective is to characterize the gene complement and stage-specific gene expression patterns in Antarctic copepods within an evolutionary context. The second objective is to measure and compare the physiological and molecular responses of juvenile copepods to variable feeding conditions. The third objective is to characterize metabolic variation within natural copepod populations. The metabolically diverse Antarctic copepods also provide an excellent opportunity to compare mechanisms regulating energy storage and utilization and to test hypotheses regarding the roles of specific genes. The field studies will aim to utilize information from an ongoing long term research program (the Palmer Long-Term Ecological Research), which complements the ongoing program and provides extensive context for this project. To make the data more useful to the research community, a database will be developed facilitating comparison of transcriptomes between copepod species. This project will provide hands-on training opportunities to graduate and undergraduate students. Efforts will be made to recruit students who are members of underrepresented minorities. Results and scientific concepts will be broadly disseminated through an expedition blog, a series of interactive animations, and public presentations. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": -55.0, "geometry": "POINT(-67.5 -65)", "instruments": null, "is_usap_dc": true, "keywords": "ARTHROPODS; AMD; PELAGIC; USA/NSF; USAP-DC; PLANKTON; West Antarctic Shelf; Amd/Us; SHIPS", "locations": "West Antarctic Shelf", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Tarrant, Ann", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e SHIPS", "repo": "NCBI", "repositories": "NCBI; R2R", "science_programs": null, "south": -70.0, "title": "Physiological Ecology of \"Herbivorous\" Antarctic Copepods", "uid": "p0010239", "west": -80.0}, {"awards": "1643532 Ponganis, Paul", "bounds_geometry": "POLYGON((163 -77,163.4 -77,163.8 -77,164.2 -77,164.6 -77,165 -77,165.4 -77,165.8 -77,166.2 -77,166.6 -77,167 -77,167 -77.1,167 -77.2,167 -77.3,167 -77.4,167 -77.5,167 -77.6,167 -77.7,167 -77.8,167 -77.9,167 -78,166.6 -78,166.2 -78,165.8 -78,165.4 -78,165 -78,164.6 -78,164.2 -78,163.8 -78,163.4 -78,163 -78,163 -77.9,163 -77.8,163 -77.7,163 -77.6,163 -77.5,163 -77.4,163 -77.3,163 -77.2,163 -77.1,163 -77))", "dataset_titles": "Blood oxygen transport and depletion in diving emperor penguins; Emperor penguin air sac oxygen", "datasets": [{"dataset_uid": "200409", "doi": "10.5061/dryad.qv9s4mwnp", "keywords": null, "people": null, "repository": "Dryad", "science_program": null, "title": "Blood oxygen transport and depletion in diving emperor penguins", "url": "https://doi.org/10.5061/dryad.qv9s4mwnp"}, {"dataset_uid": "200236", "doi": "10.5061/dryad.3tx95x6f5", "keywords": null, "people": null, "repository": "Dryad", "science_program": null, "title": "Emperor penguin air sac oxygen", "url": "https://doi.org/10.6076/D1H01Z"}], "date_created": "Fri, 30 Jul 2021 00:00:00 GMT", "description": "During exercise, oxygen must be efficiently delivered from the lungs to the working tissues. Birds have a unique respiratory system that includes both air sacs and lungs (called parabronchi) and has a one-way, rather than bidirectional, air flow pattern. This allows a high proportion of the oxygen in inhaled air to be transferred into the blood so that it can be circulated by the cardiovascular system to the tissues. In diving birds such as the emperor penguin, the air sac-to-tissue oxygen delivery is essential to the dive capacity, and is one of the adaptations that allows this species to dive deeper than 500 meters. However, the physiological mechanisms underlying the transfer of oxygen from air sacs to blood and the subsequent distribution of oxygen to tissues are poorly understood. The emperor penguin is ideal for investigation of this oxygen cascade because of its large body size, dive capacity, physiological data base, and the prior development of research techniques and protocols for this species. This study should provide insight into a) the mechanisms underlying the efficiency of the bird oxygen transport system, b) the physiological basis of penguin dive behavior, and the ability of penguins to adapt to environmental change, and c) perhaps, even the design of better therapeutic strategies and tools for treatment of respiratory disease. The project also includes educational exhibits and lecture programs on penguin biology at SeaWorld of San Diego. These educational programs at SeaWorld have outreach to diverse groups of grade school and high school students. One graduate student will also be trained, and participate in Antarctic physiological research. This project will examine the transport of oxygen from air sacs to tissues in a series of studies with temporarily captive emperor penguins that are free-diving at an isolated dive hole research camp in McMurdo Sound. Physiological data will be obtained with application of backpack recorders for the partial pressure of oxygen (PO2) in air sacs and/or blood, and backpack heart rate/stroke rate recorders. This experimental approach will lay the groundwork for future investigations of air sac to lung to blood oxygen transfer during exercise of flying and running birds. Four major topics are examined in this project: a) air sac oxygen distribution/depletion and the movement of air between anterior and posterior air sacs, b) anterior air sac to arterial PO2 differences and parabronchial gas exchange, c) blood oxygen transport and depletion throughout dives, and the nature of the aerobic dive limit, and d) the relationship of venous oxygen depletion patterns to both heart rate and stroke effort during dives. Specific educational outreach goals include a) short video features to be displayed in the Penguin Encounter exhibit at SeaWorld of San Diego, and b) lectures, video presentations, and pre- and post-course evaluations for student campers and participants in SeaWorld\u0027s education programs. Underwater video for exhibits/presentations with be obtained with use of a penguin backpack camera in the Antarctic. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 167.0, "geometry": "POINT(165 -77.5)", "instruments": null, "is_usap_dc": true, "keywords": "McMurdo Sound; USAP-DC; FIELD SURVEYS; USA/NSF; Amd/Us; AMD; PENGUINS", "locations": "McMurdo Sound", "north": -77.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Ponganis, Paul", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS", "repo": "Dryad", "repositories": "Dryad", "science_programs": null, "south": -78.0, "title": "From Air Sacs to Tissues: Oxygen Transfer and Utilization in Diving Emperor Penguins", "uid": "p0010236", "west": 163.0}, {"awards": "1643825 Bucklin, Ann", "bounds_geometry": "POLYGON((-74.57 -60.9,-72.487 -60.9,-70.404 -60.9,-68.321 -60.9,-66.238 -60.9,-64.155 -60.9,-62.072 -60.9,-59.989 -60.9,-57.906 -60.9,-55.823 -60.9,-53.74 -60.9,-53.74 -61.537,-53.74 -62.174,-53.74 -62.811,-53.74 -63.448,-53.74 -64.085,-53.74 -64.722,-53.74 -65.359,-53.74 -65.996,-53.74 -66.633,-53.74 -67.27,-55.823 -67.27,-57.906 -67.27,-59.989 -67.27,-62.072 -67.27,-64.155 -67.27,-66.238 -67.27,-68.321 -67.27,-70.404 -67.27,-72.487 -67.27,-74.57 -67.27,-74.57 -66.633,-74.57 -65.996,-74.57 -65.359,-74.57 -64.722,-74.57 -64.085,-74.57 -63.448,-74.57 -62.811,-74.57 -62.174,-74.57 -61.537,-74.57 -60.9))", "dataset_titles": "Alongtrack data collected continuously by the ship\u0027s underway acquisition system from ARSV Laurence M. Gould cruise LMG1110 in the Southern Ocean in 2011 ; Bucklin, A., R.J. O\u0027Neill, D. Payne (2018) Antarctic salp genome and RNAseq transcriptome from ARSV Laurence M. Gould, Umitaka-Maru, R/V Polarstern LMG1110, UM-08-09, ANT-XXVII-2 in the Southern Ocean. Biological and Chemical Oceanography Data Management Office (BCO-DMO). ; Bucklin, A., R.J. O\u0027Neill, D. Payne (2018) Salp specimen log for genomic and transcriptomic study collected from ARSV Laurence M. Gould, Umitaka-Maru, R/V Polarstern LMG1110, UM-08-09, ANT-XXVII-2. Biological and Chemical Oceanography Data Management Office (BCO-DMO).; CTD data from ARSV Laurence M. Gould LMG1110 in the Southern Ocean from November to December 2011 (Salp_Antarctic project) ; CTD data from MOCNESS tows taken in the Antarctic in 2011 from ARSV Laurence M. Gould LMG1110 in the Southern Ocean from November to December 2011 (Salp_Antarctic project) ; Scientific sampling event log from ARSV Laurence M. Gould LMG1110 in the Southern Ocean from Nov. 2011 (Salp_Antarctic project) ", "datasets": [{"dataset_uid": "200229", "doi": "", "keywords": null, "people": null, "repository": "BCO-DMO", "science_program": null, "title": "CTD data from ARSV Laurence M. Gould LMG1110 in the Southern Ocean from November to December 2011 (Salp_Antarctic project) ", "url": "https://www.bco-dmo.org/dataset/559174/data"}, {"dataset_uid": "200230", "doi": "", "keywords": null, "people": null, "repository": "BCO-DMO", "science_program": null, "title": "Scientific sampling event log from ARSV Laurence M. Gould LMG1110 in the Southern Ocean from Nov. 2011 (Salp_Antarctic project) ", "url": "https://www.bco-dmo.org/dataset/3565/data"}, {"dataset_uid": "200228", "doi": "", "keywords": null, "people": null, "repository": "BCO-DMO", "science_program": null, "title": "Bucklin, A., R.J. O\u0027Neill, D. Payne (2018) Antarctic salp genome and RNAseq transcriptome from ARSV Laurence M. Gould, Umitaka-Maru, R/V Polarstern LMG1110, UM-08-09, ANT-XXVII-2 in the Southern Ocean. Biological and Chemical Oceanography Data Management Office (BCO-DMO). ", "url": "https://www.bco-dmo.org/dataset/675040/data"}, {"dataset_uid": "200231", "doi": "", "keywords": null, "people": null, "repository": "BCO-DMO", "science_program": null, "title": "Alongtrack data collected continuously by the ship\u0027s underway acquisition system from ARSV Laurence M. Gould cruise LMG1110 in the Southern Ocean in 2011 ", "url": "https://www.bco-dmo.org/dataset/3636/data"}, {"dataset_uid": "200227", "doi": "", "keywords": null, "people": null, "repository": "BCO-DMO", "science_program": null, "title": "Bucklin, A., R.J. O\u0027Neill, D. Payne (2018) Salp specimen log for genomic and transcriptomic study collected from ARSV Laurence M. Gould, Umitaka-Maru, R/V Polarstern LMG1110, UM-08-09, ANT-XXVII-2. Biological and Chemical Oceanography Data Management Office (BCO-DMO).", "url": "https://www.bco-dmo.org/dataset/672600"}, {"dataset_uid": "200232", "doi": "", "keywords": null, "people": null, "repository": "BCO-DMO", "science_program": null, "title": "CTD data from MOCNESS tows taken in the Antarctic in 2011 from ARSV Laurence M. Gould LMG1110 in the Southern Ocean from November to December 2011 (Salp_Antarctic project) ", "url": "https://www.bco-dmo.org/dataset/488871/data"}], "date_created": "Sat, 03 Jul 2021 00:00:00 GMT", "description": "The Antarctic salp, Salpa thompsoni, is a gelatinous zooplankton that is an important member in the Southern Ocean pelagic ecosystem. Field studies have documented rapid population growth under favorable environmental conditions, resulting in dense blooms of salps that substantially change the pelagic ecosystem in regards to both structure and processes. Because this zooplankton can proliferate rapidly and it is not readily consumed by upper trophic levels, its periodic dominance has the potential to drastically chance ecosystem energetics as well as change material export to the deep ocean. Completion of a comprehensive reference genome for the Antarctic salp will enable the identification of genes and gene networks underlying physiological responses and allow detection of potential processes driving natural selection and the species? adaptation strategies to the Antarctic Environment. Comparative genomic analysis will add the dimension of time to inferences about organismal adaptation and allow consideration of their potential to adapt to future environmental changes, and will allow examination of novel aspects of genomic evolution found only in the invertebrate class Tunicata. The completed salp reference genome will provide a valuable foundational resource for other scientists working on this species as well as the genomic basis for function and adaptation in the Antarctic. The primary goal of this effort is to examine the rapid genome evolution characteristic of this tunicate species and examine the genomic bases of the species? potential for adaptation, and specifically the role of flexible gene networks for successful responses to changing environmental conditions. The primary hypothesis driving this research is that predicted S. thompsoni orthologs (i.e., genes of the same function that share a common ancestor) that show evidence of rapid evolution are indicative of positive selection, and further that these genes and associated gene networks provide the basis for rapid adaptation of the Antarctic salp to environmental variation associated with a changing ocean. The proposed genome assembly strategy will allow further refinements and scaffolding of the current, highly fragmented genome assembly using the methods developed during previous work. Specimens of S. thompsoni now archived at UConn will be analyzed to improve the salp genome assembly, increasing overall scaffold length, and decreasing the number of total contigs. High-quality reference assemblies will be obtained with two high-output paired-end sequencing runs (Illumina) on a single individual, coupled with three runs on the Oxford Nanopore long-read sequencer. The same sequencing strategy will be performed on a sub-sampling of tissues from the same specimen to produce a very high quality reference transcriptome, which will allow for high quality gene models and near-complete gene predictions in the genome assembly. Comparisons with available genomic data for Urochordate and Cephalochordate species will increase the number of orthologs analyzed. Orthologous genes will be tested for evidence of rapid selection in the salp lineage, and the results will be compared to published expression profiles and ontology functions for the salp. All data will be made publicly available via existing web portals; a project website will be developed to disseminate research results for access by the both research and educational communities. Website design will use a local instance of jbrowse that will offer annotations, downloadable data files, and tracts of previously-published datasets.", "east": -53.74, "geometry": "POINT(-64.155 -64.085)", "instruments": null, "is_usap_dc": true, "keywords": "SHIPS; PELAGIC; Southern Ocean", "locations": "Southern Ocean", "north": -60.9, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Bucklin, Ann; O\u0027Neill, Rachel J", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e SHIPS", "repo": "BCO-DMO", "repositories": "BCO-DMO", "science_programs": null, "south": -67.27, "title": "Genome Assembly and Analysis of the Bloom Forming Southern Ocean Salp, Salpa thompsoni", "uid": "p0010224", "west": -74.57}, {"awards": "1947882 Robel, Alexander", "bounds_geometry": null, "dataset_titles": null, "datasets": null, "date_created": "Thu, 01 Jul 2021 00:00:00 GMT", "description": "Uncertainty in projections of future sea level rise comes, in part, from ice-sheet melting under the influence of unpredictable variations in ocean and atmospheric temperature near ice sheets. Using state-of-the-art modeling techniques, the Antarctic Ice Sheet Large Ensemble (AISLENS) Project will estimate the range of possible Antarctic Ice Sheet melt during the recent past and over the next several centuries that could result from such climate variations. The AISLENS Project will also facilitate research by providing modeling output as an open product to the broader climate and glaciology communities. The project will support an early career faculty member, and interdisciplinary training for a graduate student, postdoctoral fellow and undergraduate student. As a part of this project, an undergraduate course on \"Sea Level Rise and Coastal Engineering\" will be also developed, bringing together Earth Science and Civil Engineering students in an interdisciplinary setting and contributing to their education in sea level science and coastal adaptation. This will be done in the geographic context of the Southeastern US, the region of most concentrated vulnerability to sea-level rise in the US. The primary goal of the proposed research is to understand and quantify the role of internal climate variability in driving ice loss from the Antarctic Ice Sheet over the recent past and into the future. The AISLENS Project will encompass hundreds of simulations of Antarctic ice sheet evolution from 1950 to 2300 forced by realistic variations in climate, including snowfall and melt from fluctuating oceanic and atmospheric temperatures. Plausible realizations of Antarctic climate forcing will be generated from stochastic emulation of output from the Energy Exascale Earth System Model (E3SM) under past and future emissions scenarios. These realizations of variable climate will be used to force the MPAS Albany Land Ice (MALI) model, a state-of-the-art model of ice flow in the Antarctic Ice Sheet. In this project, AISLENS will be used to conduct uncertainty and attribution analyses. In the uncertainty analysis, the evolution of ensemble spread in simulations of the future evolution of the Antarctic Ice Sheet will be systematically decomposed to determine which temporal and spatial scales of climate variability contribute the most to future ice-sheet projection uncertainty. In the attribution analysis, a range of satellite-based observations of recent Antarctic ice loss will be compared to the envelope of internal variability of Antarctic ice loss simulated in AISLENS simulations encompassing the recent past. This analysis will provide context to recent observations indicating significant variability of Antarctic climate forcing and provide a possible path forward for conducting robust statistical inference studies for observed ice-sheet changes. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "ICE SHEETS; Antarctica; Antarctic Ice Sheet; AMD; USAP-DC; USA/NSF; MODELS; Amd/Us", "locations": "Antarctic Ice Sheet; Antarctica", "north": null, "nsf_funding_programs": "Antarctic Glaciology; Antarctic Integrated System Science", "paleo_time": null, "persons": "Robel, Alexander", "platforms": "OTHER \u003e MODELS \u003e MODELS", "repositories": null, "science_programs": null, "south": null, "title": "The Antarctic Ice Sheet Large Ensemble (AISLENS) Project: Assessing the Role of Climate Variability in Past and Future Ice Sheet Mass Loss", "uid": "p0010223", "west": null}, {"awards": "1935672 Ryan, Joseph; 1935635 Santagata, Scott", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": null, "datasets": null, "date_created": "Mon, 28 Jun 2021 00:00:00 GMT", "description": "The Antarctic benthic marine invertebrate communities are currently experiencing rapid environmental change due to the combined effects of global warming, ocean acidification, and the potential for ice-shelf collapse. Colonial invertebrate animals called bryozoans create specialized \u2018reef-like\u2019 habitats that are reminiscent of the coral reefs found in tropical marine environments. In the Antarctic, these bryozoan communities occupy significant portions of the shallow and deep seafloor, and provide habitat for other marine animals. The bryozoan lineages that make up these communities have undergone dramatic genetic and physiological changes in response to the unique environmental conditions found in Antarctica. Comparison of the DNA data from multiple Antarctic bryozoans to those of related warm-water species will help researchers identify unique and shared adaptations characteristic of bryozoans and other marine organisms that have adapted to the Antarctic environment. Additionally, direct experimental tests of catalytic-related genes (enzymes) will shed light on potential cold-adaption in various cell processes. Workshops will train diverse groups of scientists using computational tools to identify genetic modifications of organisms from disparate environments. Public outreach activities to students, social media, and science journalists are designed to raise awareness and appreciation of the spectacular marine life in the Antarctic and the hidden beauty of bryozoan biology. Understanding the genomic changes underlying adaptations to polar environments is critical for predicting how ecological changes will affect life in these fragile environments. Accomplishing these goals requires looking in detail at genome-scale data across a wide array of organisms in a phylogenetic framework. This study combines multifaceted computational and functional approaches that involves analyzing in the genic evolution of invertebrate organisms, known as the bryozoans or ectoprocts. In addition, the commonality of bryozoan results with those of other taxa will be tested by comparing newly generated data to that produced in previous workshops. The specific aims of this study include: 1) identifying genes involved in adaptation to Antarctic marine environments using transcriptomic and genomic data from bryozoans to test for positively selected genes in a phylogenetic framework, 2) experimentally testing identified candidate enzymes (especially those involved in calcium signaling, glycolysis, the citric acid cycle, and the cytoskeleton) for evidence of cold adaption, and 3) conducting computational workshops aimed at training scientists in techniques for the identification of genetic adaptations to polar and other disparate environments. The proposed work provides critical insights into the molecular rules of life in rapidly changing Antarctic environments, and provides important information for understanding how Antarctic taxa will respond to future environmental conditions. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "USAP-DC; USA/NSF; Ross Sea; Ant Lia; ANIMALS/INVERTEBRATES; FIELD SURVEYS; Weddell Sea; Bellingshausen Sea; Amundsen Sea; Antarctic Peninsula; Amd/Us; AMD", "locations": "Amundsen Sea; Antarctic Peninsula; Bellingshausen Sea; Ross Sea; Weddell Sea", "north": -60.0, "nsf_funding_programs": "Antarctic Integrated System Science; Antarctic Integrated System Science; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Ryan, Joseph; Santagata, Scott", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS", "repositories": null, "science_programs": null, "south": -90.0, "title": "ANT LIA Collaborative Research: Interrogating Molecular and Physiological Adaptations in Antarctic Marine Animals.", "uid": "p0010212", "west": -180.0}, {"awards": "1906015 Kelley, Joanna", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "Data, Code, and Results for the Zoarcoidei Phylogeny (Hotaling et al.)", "datasets": [{"dataset_uid": "200221", "doi": "10.5281/zenodo.4306092).", "keywords": null, "people": null, "repository": "Zenodo", "science_program": null, "title": "Data, Code, and Results for the Zoarcoidei Phylogeny (Hotaling et al.)", "url": "https://doi.org/10.5281/zenodo.4306092"}], "date_created": "Fri, 25 Jun 2021 00:00:00 GMT", "description": "Fish that reside in the harsh, subfreezing waters of the Antarctic and Arctic provide fascinating examples of adaptation to extreme environments. Species at both poles have independently evolved ways to deal with constant cold temperature, including the evolution of antifreeze proteins. Under freezing conditions, these compounds attach to ice crystals and prevent their growth. This lowers the tissue freezing point and reduces the chance the animal will be injured or killed. While it might seem that the need for unique adaptations to survive in polar waters would reduce species diversity in these habitats, recent evidence showed higher speciation rates in fishes from polar environments as compared to those found in warmer waters. This is despite the fact cold temperatures slow cellular processes, which had been expected to lower rates of molecular evolution in these species. To determine how rates of speciation and molecular evolution are linked in marine fishes, this project will compare the genomes of multiple polar and non-polar fishes. By doing so, it will (1) clarify how rates of evolution vary in polar environments, (2) identify general trends that shape the adaptive trajectories of polar fishes, and (3) determine how functional differences shape the evolution of novel compounds such as the antifreeze proteins some polar fishes rely upon to survive. In addition to training a new generation of scientists, the project will develop curriculum and outreach activities for elementary and undergraduate science courses. Materials will be delivered in classrooms across the western United States, with a focus on rural schools as part of a network for promoting evolutionary education in rural communities. To better understand the biology of polar fishes and the evolution of antifreeze proteins (AFPs), this research will compare the evolutionary histories of cold-adapted organisms to those of related non-polar species from both a genotypic and phenotypic context. In doing so, this research will test whether evolutionary rates are slowed in polar environments, perhaps due to constraints on cellular processes. It will also evaluate the effects of positive selection and the relaxation of selection on genes and pathways, both of which appear to be key adaptive strategies involved in the adaptation to polar environments. To address specific mechanisms by which extreme adaptation occurs, researchers will determine how global gradients of temperature and dissolved oxygen shape genome variation and influence adaptive trajectories among multiple species of eelpouts (family Zoarcidae). An in-vitro experimental approach will then be used to test functional hypotheses about the role of copy number variation in AFP evolution, and how and why multiple antifreeze protein isoforms have evolved. By comparing the genomes of multiple polar and non-polar fishes, the project will clarify how rates of evolution vary in polar environments, identify general trends that shape the adaptive trajectories of cold-adapted marine fishes, and determine how functional differences shape the evolution of novel proteins. This project addresses the strategic programmatic aim to provide a better understanding of the genetic underpinnings of organismal adaptations to their current environment and ways in which polar fishes may respond to changing conditions over different evolutionary time scales. The project is jointly funded by the Antarctic Organisms and Ecosystems Program in the Office of Polar Programs of the Geosciences Directorate, and the Molecular Biophysics Program of the Division of Molecular and Cellular Biosciences in the Biological Sciences Directorate. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "Antarctica; FISH; MARINE ECOSYSTEMS; LABORATORY; AMD; USAP-DC; Amd/Us; USA/NSF", "locations": "Antarctica", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Kelley, Joanna", "platforms": "OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repo": "Zenodo", "repositories": "Zenodo", "science_programs": null, "south": -90.0, "title": "Genome Evolution in Polar Fishes", "uid": "p0010200", "west": -180.0}, {"awards": "1850988 Teets, Nicholas", "bounds_geometry": "POLYGON((-64.366767 -62.68104,-63.9917036 -62.68104,-63.6166402 -62.68104,-63.2415768 -62.68104,-62.8665134 -62.68104,-62.49145 -62.68104,-62.1163866 -62.68104,-61.7413232 -62.68104,-61.3662598 -62.68104,-60.9911964 -62.68104,-60.616133 -62.68104,-60.616133 -62.9537037,-60.616133 -63.2263674,-60.616133 -63.4990311,-60.616133 -63.7716948,-60.616133 -64.0443585,-60.616133 -64.3170222,-60.616133 -64.5896859,-60.616133 -64.8623496,-60.616133 -65.1350133,-60.616133 -65.407677,-60.9911964 -65.407677,-61.3662598 -65.407677,-61.7413232 -65.407677,-62.1163866 -65.407677,-62.49145 -65.407677,-62.8665134 -65.407677,-63.2415768 -65.407677,-63.6166402 -65.407677,-63.9917036 -65.407677,-64.366767 -65.407677,-64.366767 -65.1350133,-64.366767 -64.8623496,-64.366767 -64.5896859,-64.366767 -64.3170222,-64.366767 -64.0443585,-64.366767 -63.7716948,-64.366767 -63.4990311,-64.366767 -63.2263674,-64.366767 -62.9537037,-64.366767 -62.68104))", "dataset_titles": "Belgica antarctica collection sites - Summer 2023/2024 field season; Cold and dehydration tolerance of Belgica antarctica from three distinct geographic locations; Cross-tolerance in Belgica antarctica near Palmer Peninsula; Data from Edgington, H., Pavinato, V.A.C., Spacht, D., Gantz, J.D., Convey, P., Lee, R.E., Denlinger, D.L., Michel, A., 2023. Genetic history, structure and gene flow among populations of Belgica antarctica, the only free-living insect in the western Antarctic Peninsula. Polar Science 36, 100945.; Data from microplastics exposure in Belgica antarctica; Fine\u2011scale variation in microhabitat conditions influences physiology and metabolism in an Antarctic insect; Information on 2023 collection sites for Belgica antarctica; LMG2002 Expedtition Data; Long-term recovery from freezing in Belgica antarctica; Multiple stress tolerance in the Antarctic midge; Simulated winter warming negatively impacts survival of Antarcticas only endemic insect; Stress tolerance in Belgica antarctica and Eretmoptera murphyi; Temporal and spatial variation in stress tolerance in Belgica antarctica populations from distinct islands", "datasets": [{"dataset_uid": "601873", "doi": null, "keywords": "Antarctica; Belgica Antarctica; Cryosphere; Population Genetics", "people": "Michel, Andrew; Sousa Lima, Cleverson; Teets, Nicholas; Hayward, Scott", "repository": "USAP-DC", "science_program": null, "title": "Temporal and spatial variation in stress tolerance in Belgica antarctica populations from distinct islands", "url": "https://www.usap-dc.org/view/dataset/601873"}, {"dataset_uid": "601875", "doi": null, "keywords": "Antarctica; Cryosphere", "people": "Michel, Andrew; Sousa Lima, Cleverson; Hayward, Scott; Teets, Nicholas", "repository": "USAP-DC", "science_program": null, "title": "Belgica antarctica collection sites - Summer 2023/2024 field season", "url": "https://www.usap-dc.org/view/dataset/601875"}, {"dataset_uid": "601872", "doi": "10.15784/601872", "keywords": "Antarctica; Belgica Antarctica; Cryosphere", "people": "Colinet, Herve; Teets, Nicholas; Sousa Lima, Cleverson; Michel, Andrew; Hayward, Scott", "repository": "USAP-DC", "science_program": null, "title": "Cross-tolerance in Belgica antarctica near Palmer Peninsula", "url": "https://www.usap-dc.org/view/dataset/601872"}, {"dataset_uid": "601871", "doi": null, "keywords": "Antarctica; Belgica Antarctica; Cryosphere", "people": "Kawarasaki, Yuta; Pavinato, Vitor; Gantz, Josiah D.; Devlin, Jack; Michel, Andrew; Teets, Nicholas; Hayward, Scott; Sousa Lima, Cleverson; Aquilino, Monica", "repository": "USAP-DC", "science_program": null, "title": "Stress tolerance in Belgica antarctica and Eretmoptera murphyi", "url": "https://www.usap-dc.org/view/dataset/601871"}, {"dataset_uid": "601867", "doi": null, "keywords": "Antarctica; Antarctic Peninsula; Cryosphere", "people": "Teets, Nicholas", "repository": "USAP-DC", "science_program": null, "title": "Multiple stress tolerance in the Antarctic midge", "url": "https://www.usap-dc.org/view/dataset/601867"}, {"dataset_uid": "601866", "doi": null, "keywords": "Antarctica; Antarctic Peninsula; Cryosphere", "people": "Teets, Nicholas; Devlin, Jack", "repository": "USAP-DC", "science_program": null, "title": "Data from microplastics exposure in Belgica antarctica", "url": "https://www.usap-dc.org/view/dataset/601866"}, {"dataset_uid": "601865", "doi": "10.15784/601865", "keywords": "Antarctica; Antarctic Peninsula; Cryosphere; Seasonality", "people": "Spacht, Drew; Denlinger, David; Lee, Richard; McCabe, Eleanor; Devlin, Jack; Gantz, Josiah D.; Teets, Nicholas", "repository": "USAP-DC", "science_program": null, "title": "Fine\u2011scale variation in microhabitat conditions influences physiology and metabolism in an Antarctic insect", "url": "https://www.usap-dc.org/view/dataset/601865"}, {"dataset_uid": "601864", "doi": "10.15784/601864", "keywords": "Antarctica; Antarctic Peninsula; Cryosphere", "people": "Teets, Nicholas; Kawarasaki, Yuta", "repository": "USAP-DC", "science_program": null, "title": "Cold and dehydration tolerance of Belgica antarctica from three distinct geographic locations", "url": "https://www.usap-dc.org/view/dataset/601864"}, {"dataset_uid": "200222", "doi": "10.7284/908802", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "LMG2002 Expedtition Data", "url": "https://www.rvdata.us/search/cruise/LMG2002"}, {"dataset_uid": "200425", "doi": "", "keywords": null, "people": null, "repository": "USAP-DC", "science_program": null, "title": "Simulated winter warming negatively impacts survival of Antarcticas only endemic insect", "url": "https://www.usap-dc.org/view/dataset/601694"}, {"dataset_uid": "200437", "doi": "", "keywords": null, "people": null, "repository": "USAP-DC", "science_program": null, "title": "Stress tolerance in Belgica antarctica and Eretmoptera murphyi", "url": "https://www.usap-dc.org/view/dataset/601874"}, {"dataset_uid": "200438", "doi": "", "keywords": null, "people": null, "repository": "NCBI", "science_program": null, "title": "Data from Edgington, H., Pavinato, V.A.C., Spacht, D., Gantz, J.D., Convey, P., Lee, R.E., Denlinger, D.L., Michel, A., 2023. Genetic history, structure and gene flow among populations of Belgica antarctica, the only free-living insect in the western Antarctic Peninsula. Polar Science 36, 100945.", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA565153/"}, {"dataset_uid": "601687", "doi": "10.15784/601687", "keywords": "Antarctica; Antarctic Peninsula; Belgica Antarctica; Biota; Sample Location", "people": "Teets, Nicholas; Michel, Andrew; Peter, Convey; Sousa Lima, Cleverson; Pavinato, Vitor; Devlin, Jack; Kawarasaki, Yuta; Gantz, Joseph", "repository": "USAP-DC", "science_program": null, "title": "Information on 2023 collection sites for Belgica antarctica", "url": "https://www.usap-dc.org/view/dataset/601687"}, {"dataset_uid": "601698", "doi": "10.15784/601698", "keywords": "Antarctica; Belgica Antarctica; Palmer Station", "people": "Devlin, Jack; Teets, Nicholas; Sousa Lima, Cleverson; Lecheta, Melise", "repository": "USAP-DC", "science_program": null, "title": "Long-term recovery from freezing in Belgica antarctica", "url": "https://www.usap-dc.org/view/dataset/601698"}], "date_created": "Fri, 25 Jun 2021 00:00:00 GMT", "description": "The cold, dry terrestrial environments of Antarctica are inhospitable for insects, and only three midge species make Antarctica home. Of these, Belgica antarctica is the only species found exclusively in Antarctica, and it has been a resident of Antarctica since the continent split from South America ~30 million years ago. Thus, this species is an excellent system to model the biological history of Antarctica throughout its repeated glaciation events and shifts in climate. This insect is also a classic example of extreme adaptation, and much previous work has focused on identifying the genetic and physiological mechanisms that allow this species to survive where no other insect is capable. However, it has been difficult to pinpoint the unique evolutionary adaptations that are required to survive in Antarctica due to a lack of information from closely related Antarctic and sub-Antarctic species. This project will compare adaptations, genome sequences, and population characteristics of four midge species that span an environmental gradient from sub-Antarctic to Antarctic habitats. In addition to B. antarctica, these species include two species that are strictly sub-Antarctic and a third that is native to the sub-Antarctic but has invaded parts of Antarctica. The researchers, comprised of scientists from the US, UK, Chile, and France, will sample insects from across their geographic range and measure their ability to tolerate environmental stressors (i.e., cold and desiccation), quantify molecular responses to stress, and compare the makeup of the genome and patterns of genetic diversity. This research will contribute to a greater understanding of adaptation to extremes, to an understanding of biodiversity on the planet and to understanding and predicting changes accompanying environmental change. The project will train two graduate students and two postdoctoral researchers, and a K-12 educator will be a member of the field team and will assist with fieldwork and facilitate outreach with schools in the US. The project includes partnership activities with several STEM education organizations to deliver educational content to K-12 and secondary students. This is a project that is jointly funded by the National Science Foundation\u0027s Directorate of Geosciences (NSF/GEO) and the National Environment Research Council (NERC) of the United Kingdom (UK) via the NSF/GEO-NERC Lead Agency Agreement. This Agreement allows a single joint US/UK proposal to be submitted and peer-reviewed by the Agency whose investigator has the largest proportion of the budget. Each Agency funds the proportion of the budget and the investigators associated with its own country. UK participation in this project includes deploying scientists as part of the field team, supporting field and sampling logistics at remote Antarctic sites, and genome sequencing, annotation, and analyses. This project focuses on the key physiological adaptations and molecular processes that allow a select few insect species to survive in Antarctica. The focal species are all wingless with limited dispersal capacity, suggesting there is also significant potential to locally adapt to variable environmental conditions across the range of these species. The central hypothesis is that similar molecular mechanisms drive both population-level adaptation to local environmental conditions and macroevolutionary changes across species living in different environments. The specific aims of the project are to 1) Characterize conserved and species-specific adaptations to extreme environments through comparative physiology and transcriptomics, 2) Compare the genome sequences of these species to identify genetic signatures of extreme adaption, and 3) Investigate patterns of diversification and local adaptation across each species? range using population genomics. The project establishes an international collaboration of researchers from the US, UK, Chile, and France with shared interests and complementary expertise in the biology, genomics, and conservation of Antarctic arthropods. The Broader Impacts of the project include training students and partnering with the Living Arts and Science Center to design and implement educational content for K-12 students. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": -60.616133, "geometry": "POINT(-62.49145 -64.0443585)", "instruments": null, "is_usap_dc": true, "keywords": "Antarctic Peninsula; Livingston Island; Antarctica; USAP-DC; AMD; R/V LMG; USA/NSF; ARTHROPODS; Amd/Us; Anvers Island", "locations": "Antarctica; Antarctic Peninsula; Anvers Island; Livingston Island", "north": -62.68104, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Teets, Nicholas; Michel, Andrew", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "USAP-DC", "repositories": "NCBI; R2R; USAP-DC", "science_programs": null, "south": -65.407677, "title": "NSFGEO-NERC: Mechanisms of Adaptation to Terrestrial Antarctica through Comparative Physiology and Genomics of Antarctic and sub-Antarctic Insects", "uid": "p0010203", "west": -64.366767}, {"awards": "1543539 Liwanag, Heather", "bounds_geometry": null, "dataset_titles": "metabolic measurements; Sedation dose and response; TDR and weather data", "datasets": [{"dataset_uid": "601631", "doi": "10.15784/601631", "keywords": "Antarctica; McMurdo Sound; Weddell Seal", "people": "Pearson, Linnea", "repository": "USAP-DC", "science_program": null, "title": "Sedation dose and response", "url": "https://www.usap-dc.org/view/dataset/601631"}, {"dataset_uid": "601435", "doi": "10.15784/601435", "keywords": "Antarctica; McMurdo Sound; Weddell Seal", "people": "Liwanag, Heather; Weitzner, Emma; Pearson, Linnea", "repository": "USAP-DC", "science_program": null, "title": "TDR and weather data", "url": "https://www.usap-dc.org/view/dataset/601435"}, {"dataset_uid": "601524", "doi": "10.15784/601524", "keywords": "Antarctica; McMurdo Sound; Metabolic Rate; Thermoregulation; Weddell Seal", "people": "Pearson, Linnea", "repository": "USAP-DC", "science_program": null, "title": "metabolic measurements", "url": "https://www.usap-dc.org/view/dataset/601524"}], "date_created": "Sat, 12 Dec 2020 00:00:00 GMT", "description": "The transition of young from parental care to independence is a critical stage in the life of many animals. Surviving this stage can be especially challenging for polar mammals where the extreme cold requires extra energy to keep warm, rather than using the majority of energy for growth, development and physical activities. Young Weddell seals (Leptonychotes weddellii) have only weeks to develop the capabilities to survive both on top of the sea ice and within the -1.9\u00b0C seawater where they can forage for food. The project seeks to better understand how Weddell seal pups rapidly develop (within weeks) the capacity to transition between these two extreme environments (that differ greatly in their abilities to conduct heat) and how they budget their energy during the transition. Though the biology and physiology of adult Weddell seals is well studied, the energetic and physiological strategies of pups during development is still unclear. Understanding factors that may affect survival at critical life history events is essential for better understanding factors that might affect marine mammal populations. Weddell seals are the southernmost breeding mammal and are easily recognizable as quintessential Antarctic seals. Determining potential vulnerabilities at critical life stages to change in the Antarctic environment will facilitate the researchers\u0027 ability to not only gain public interest but also communicate how research is revealing ways in which changes are occurring at the poles and how these changes may affect polar ecosystems. By collaborating with the Marine Mammal Center, the project will directly reach the public, through curricular educational materials and public outreach that will impact over 100,000 visitors annually. To elucidate the physiological strategies that facilitate the survival of Weddell seal pups from birth to independence, the proposed study examines the development of their thermoregulation and diving capability. To achieve this, the project will determine the mechanisms that Weddell seal pups use to maintain a stable, warm body temperature in air and in water and then examine the development of diving capability as the animals prepare for independent foraging. The researchers will take a fully integrative approach- making assessments from proteins to tissues to the whole-animal level- when investigating both these objectives. To assess the development of thermoregulatory capability, researchers will quantify body insulation, resting metabolic rates in air and in water, muscle thermogenesis (shivering), and body surface temperatures in the field. The project will also assess the development of dive capability by quantifying oxygen storage capacities and measuring early dive behavior. To identify possible cellular mechanisms for how Weddell seals navigate this trade-off during development, the program will quantify several key developmental regulators of increased hypoxic capacity (HIF, VEGF and EPO) using qPCR, as well as follow the proteomic changes of adipose and muscle tissue, which will include abundance changes of metabolic, antioxidant, cytoskeletal, and Ca2+-regulating proteins. The study of the physiological development leading up to the transition to independence in pinnipeds will help researchers better predict the effects of climate change on the distribution and abundance of this species and how this will affect other trophic levels. Environmental changes that alter habitat suitability have been shown to decrease population health, specifically because of declines in juvenile survival.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "MAMMALS; FIELD INVESTIGATION; McMurdo Sound", "locations": "McMurdo Sound", "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Liwanag, Heather; Pearson, Linnea; Tomanek, Lars", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": null, "title": "RUI: Growing Up on Ice: Physiological Adaptations and Developmental Plasticity in Weddell Seal Pups Across Two Extreme Physical Environments", "uid": "p0010144", "west": null}, {"awards": "0732450 Van Dover, Cindy; 0732983 Vernet, Maria; 0732917 McCormick, Michael; 0732711 Smith, Craig", "bounds_geometry": "POLYGON((-60.5 -63.1,-59.99 -63.1,-59.48 -63.1,-58.97 -63.1,-58.46 -63.1,-57.95 -63.1,-57.44 -63.1,-56.93 -63.1,-56.42 -63.1,-55.91 -63.1,-55.4 -63.1,-55.4 -63.29,-55.4 -63.48,-55.4 -63.67,-55.4 -63.86,-55.4 -64.05,-55.4 -64.24,-55.4 -64.43,-55.4 -64.62,-55.4 -64.81,-55.4 -65,-55.91 -65,-56.42 -65,-56.93 -65,-57.44 -65,-57.95 -65,-58.46 -65,-58.97 -65,-59.48 -65,-59.99 -65,-60.5 -65,-60.5 -64.81,-60.5 -64.62,-60.5 -64.43,-60.5 -64.24,-60.5 -64.05,-60.5 -63.86,-60.5 -63.67,-60.5 -63.48,-60.5 -63.29,-60.5 -63.1))", "dataset_titles": "LARISSA: Impact of ice-shelf loss on geochemical profiles and microbial community composition in marine sediments of the Larsen A embayment, Antarctic Peninsula; NBP1001 cruise data; NBP1203 cruise data; Species Abundance Data from the Larsen Ice Shelf Ice acquired during R/V Nathaniel B. Palmer expedition NBP1203", "datasets": [{"dataset_uid": "000143", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "NBP1203 cruise data", "url": "https://www.rvdata.us/search/cruise/NBP1203"}, {"dataset_uid": "000142", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "NBP1001 cruise data", "url": "https://www.rvdata.us/search/cruise/NBP1001"}, {"dataset_uid": "601073", "doi": "10.15784/601073", "keywords": "Antarctica; Antarctic Peninsula; Chemistry:ice; Chemistry:Ice; Geochemistry; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Ice Core Records; LARISSA; Microbiology", "people": "McCormick, Michael", "repository": "USAP-DC", "science_program": null, "title": "LARISSA: Impact of ice-shelf loss on geochemical profiles and microbial community composition in marine sediments of the Larsen A embayment, Antarctic Peninsula", "url": "https://www.usap-dc.org/view/dataset/601073"}, {"dataset_uid": "601304", "doi": null, "keywords": "Antarctica; Antarctic Peninsula; Biota; Box Corer; LARISSA; Larsen Ice Shelf; Macrofauna; Megafauna; NBP1203; Oceans; R/v Nathaniel B. Palmer; Seafloor Sampling; Species Abundance", "people": "Smith, Craig", "repository": "USAP-DC", "science_program": "LARISSA", "title": "Species Abundance Data from the Larsen Ice Shelf Ice acquired during R/V Nathaniel B. Palmer expedition NBP1203", "url": "https://www.usap-dc.org/view/dataset/601304"}], "date_created": "Fri, 09 Oct 2020 00:00:00 GMT", "description": "A profound transformation in ecosystem structure and function is occurring in coastal waters of the western Weddell Sea, with the collapse of the Larsen B ice shelf. This transformation appears to be yielding a redistribution of energy flow between chemoautotrophic and photosynthetic production, and to be causing the rapid demise of the extraordinary seep ecosystem discovered beneath the ice shelf. This event provides an ideal opportunity to examine fundamental aspects of ecosystem transition associated with climate change. We propose to test the following hypotheses to elucidate the transformations occurring in marine ecosystems as a consequence of the Larsen B collapse: (1) The biogeographic isolation and sub-ice shelf setting of the Larsen B seep has led to novel habitat characteristics, chemoautotrophically dependent taxa and functional adaptations. (2) Benthic communities beneath the former Larsen B ice shelf are fundamentally different from assemblages at similar depths in the Weddell sea-ice zone, and resemble oligotrophic deep-sea communities. Larsen B assemblages are undergoing rapid change. (3) The previously dark, oligotrophic waters of the Larsen B embayment now support a thriving phototrophic community, with production rates and phytoplankton composition similar to other productive areas of the Weddell Sea. To document rapid changes occurring in the Larsen B ecosystem, we will use a remotely operated vehicle, shipboard samplers, and moored sediment traps. We will characterize microbial, macrofaunal and megafaunal components of the seep community; evaluate patterns of surface productivity, export flux, and benthic faunal composition in areas previously covered by the ice shelf, and compare these areas to the open sea-ice zone. These changes will be placed within the geological, glaciological and climatological context that led to ice-shelf retreat, through companion research projects funded in concert with this effort. Together these projects will help predict the likely consequences of ice-shelf collapse to marine ecosystems in other regions of Antarctica vulnerable to climate change. The research features international collaborators from Argentina, Belgium, Canada, Germany, Spain and the United Kingdom. The broader impacts include participation of a science writer; broadcast of science segments by members of the Jim Lehrer News Hour (Public Broadcasting System); material for summer courses in environmental change; mentoring of graduate students and postdoctoral fellows; and showcasing scientific activities and findings to students and public through podcasts.", "east": -55.4, "geometry": "POINT(-57.95 -64.05)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "NBP1203; USAP-DC; Amd/Us; LARISSA; Larsen Ice Shelf; Species Abundance Data; R/V NBP; Antarctic Peninsula; NBP1001; USA/NSF; AMD; Antarctica; MARINE ECOSYSTEMS", "locations": "Antarctica; Antarctic Peninsula; Larsen Ice Shelf", "north": -63.1, "nsf_funding_programs": "Antarctic Integrated System Science; Antarctic Organisms and Ecosystems; Antarctic Integrated System Science; Antarctic Integrated System Science; Antarctic Organisms and Ecosystems; Antarctic Integrated System Science", "paleo_time": null, "persons": "McCormick, Michael; Vernet, Maria; Van Dover, Cindy; Smith, Craig", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repo": "R2R", "repositories": "R2R; USAP-DC", "science_programs": "LARISSA", "south": -65.0, "title": "Collaborative Research in IPY: Abrupt Environmental Change in the Larsen Ice Shelf System, a Multidisciplinary Approach - Marine Ecosystems.", "uid": "p0010135", "west": -60.5}, {"awards": "9978236 Bell, Robin", "bounds_geometry": "POLYGON((101 -75.5,101.9 -75.5,102.8 -75.5,103.7 -75.5,104.6 -75.5,105.5 -75.5,106.4 -75.5,107.3 -75.5,108.2 -75.5,109.1 -75.5,110 -75.5,110 -75.85,110 -76.2,110 -76.55,110 -76.9,110 -77.25,110 -77.6,110 -77.95,110 -78.3,110 -78.65,110 -79,109.1 -79,108.2 -79,107.3 -79,106.4 -79,105.5 -79,104.6 -79,103.7 -79,102.8 -79,101.9 -79,101 -79,101 -78.65,101 -78.3,101 -77.95,101 -77.6,101 -77.25,101 -76.9,101 -76.55,101 -76.2,101 -75.85,101 -75.5))", "dataset_titles": "SOAR-Lake Vostok Survey airborne radar data; SOAR-Lake Vostok Survey bed elevation data; SOAR-Lake Vostok Survey Gravity data; SOAR-Lake Vostok Survey ice thickness data; SOAR-Lake Vostok survey magnetic anomaly data; SOAR-Lake Vostok Survey surface elevation data", "datasets": [{"dataset_uid": "601296", "doi": " 10.1594/IEDA/306564", "keywords": "Airborne Magnetic; Airplane; Antarctica; East Antarctica; Glaciers/ice Sheet; Glaciers/Ice Sheet; Lake Vostok; Magnetic; Magnetic Anomaly; Magnetometer; Potential Field; SOAR; Solid Earth", "people": "Bell, Robin; Studinger, Michael S.", "repository": "USAP-DC", "science_program": null, "title": "SOAR-Lake Vostok survey magnetic anomaly data", "url": "https://www.usap-dc.org/view/dataset/601296"}, {"dataset_uid": "601297", "doi": "10.1594/IEDA/306567", "keywords": "Airborne Laser Altimeters; Airborne Radar; Airplane; Antarctica; East Antarctica; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Ice; Ice Sheet; Ice Stratigraphy; Ice Thickness; Ice Thickness Distribution; Lake Vostok; Radar; Radar Altimetry; Radar Echo Sounder; SOAR; Subglacial Lake", "people": "Studinger, Michael S.; Bell, Robin", "repository": "USAP-DC", "science_program": null, "title": "SOAR-Lake Vostok Survey ice thickness data", "url": "https://www.usap-dc.org/view/dataset/601297"}, {"dataset_uid": "601300", "doi": "10.1594/IEDA/306568", "keywords": "Airborne Radar; Airplane; Antarctica; East Antarctica; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Lake Vostok; Navigation; Radar; SOAR; Subglacial Lakes", "people": "Bell, Robin; Studinger, Michael S.", "repository": "USAP-DC", "science_program": null, "title": "SOAR-Lake Vostok Survey airborne radar data", "url": "https://www.usap-dc.org/view/dataset/601300"}, {"dataset_uid": "601299", "doi": "10.1594/IEDA/306565", "keywords": "Airborne Laser Altimeters; Airborne Laser Altimetry; Airborne Radar; Airplane; Antarctica; Bed Elevation; Bedrock Elevation; Digital Elevation Model; East Antarctica; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Ice Sheet; Lake Vostok; Radar; Radar Echo Sounder; SOAR", "people": "Studinger, Michael S.; Bell, Robin", "repository": "USAP-DC", "science_program": null, "title": "SOAR-Lake Vostok Survey bed elevation data", "url": "https://www.usap-dc.org/view/dataset/601299"}, {"dataset_uid": "601298", "doi": "10.1594/IEDA/306566", "keywords": "Airborne Altimetry; Airborne Laser Altimeters; Airborne Radar; Airplane; Antarctica; East Antarctica; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Ice Sheet; Ice Sheet Elevation; Ice Surface; Lake Vostok; Radar Echo Sounder; SOAR; Surface Elevation", "people": "Studinger, Michael S.; Bell, Robin", "repository": "USAP-DC", "science_program": null, "title": "SOAR-Lake Vostok Survey surface elevation data", "url": "https://www.usap-dc.org/view/dataset/601298"}, {"dataset_uid": "601295", "doi": "10.1594/IEDA/306563", "keywords": "Airborne Gravity; Airplane; Antarctica; East Antarctica; Free Air Gravity; Glaciers/ice Sheet; Glaciers/Ice Sheet; Gravimeter; Gravity; Lake Vostok; Potential Field; Solid Earth", "people": "Studinger, Michael S.; Bell, Robin", "repository": "USAP-DC", "science_program": null, "title": "SOAR-Lake Vostok Survey Gravity data", "url": "https://www.usap-dc.org/view/dataset/601295"}], "date_created": "Fri, 24 Apr 2020 00:00:00 GMT", "description": "9978236 Bell Abstract This award, provided by the Office of Polar Programs under the Life in Extreme Environments (LExEn) Program, supports a geophysical study of Lake Vostok, a large lake beneath the East Antarctic Ice Sheet. Subglacial ecosystems, in particular subglacial lake ecosystems are extreme oligotrophic environments. These environments, and the ecosystems which may exist within them, should provide key insights into a range of fundamental questions about the development of Earth and other bodies in the Solar System including: 1) the processes associated with rapid evolutionary radiation after the extensive Neoproterozoic glaciations; 2) the overall carbon cycle through glacial and interglacial periods; and 3) the possible adaptations organisms may require to thrive in environments such as on Europa, the ice covered moon of Jupiter. Over 70 subglacial lakes have been identified beneath the 3-4 kilometer thick ice of Antarctica. One lake, Lake Vostok, is sufficiently large to be clearly identified from space with satellite altimetry. Lake Vostok is similar to Lake Ontario in area but with a much larger volume including measured water depths of 600 meters. The overlying ice sheet is acting as a conveyer belt continually delivering new water, nutrients, gas hydrates, sediments and microbes as the ice sheet flows across the lake. The goal of this program is to determine the fundamental boundary conditions for this subglacial lake as an essential first step toward understanding the physical processes within the lake. An aerogeophysical survey over the lake and into the surrounding regions will be acquired to meet this goal. This data set includes gravity, magnetic, laser altimetry and ice penetrating radar data and will be used to compile a basic set of ice surface elevation, subglacial topography, gravity and magnetic anomaly maps. Potential field methods widely used in the oil industry will be modified to estimate the subglacial topography from gravity data where the ice penetrating radar will be unable to recover the depth of the lake. A similar method can be modified to estimate the thickness of the sediments beneath the lake from magnetic data. These methods will be tested and applied to subglacial lakes near South Pole prior to the Lake Vostok field campaign and will provide valuable comparisons to the planned survey. Once the methods have been adjusted for the Lake Vostok application, maps of the water cavity and sediment thickness beneath the lake will be produced. These maps will become tools to explore the geologic origin of the lake. The two endmember models are, first, that the lake is an active tectonic rift such as Lake Baikal and, second, the lake is the result of glacial scouring. The distinct characteristics of an extensional rift can be easily identified with our aerogeophysical survey. The geological interpretation of the airborne geophysical survey will provide the first geological constraints of the interior of the East Antarctic continent based on modern data. In addition, the underlying geology will influence the ecosystem within the lake. One of the critical issues for the ecosystem within the lake will be the flux of nutrients. A preliminary estimation of the regions of freezing and melting based on the distance between distinctive internal layers observed on the radar data will be made. These basic boundary conditions will provide guidance for a potential international effort aimed at in situ exploration of the lake and improve the understanding of East Antarctic geologic structures.", "east": 110.0, "geometry": "POINT(105.5 -77.25)", "instruments": "EARTH REMOTE SENSING INSTRUMENTS \u003e ACTIVE REMOTE SENSING \u003e PROFILERS/SOUNDERS \u003e RADAR SOUNDERS \u003e RADAR ECHO SOUNDERS; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e MAGNETIC FIELD/ELECTRIC FIELD INSTRUMENTS \u003e MAGNETOMETERS \u003e MGF; IN SITU/LABORATORY INSTRUMENTS \u003e MAGNETIC/MOTION SENSORS \u003e GRAVIMETERS \u003e AIRGRAV", "is_usap_dc": true, "keywords": "Gravity; GLACIERS/ICE SHEETS; East Antarctica; USAP-DC; Lake Vostok; Airborne Radar; Subglacial Lake; MAGNETIC FIELD; GRAVITY", "locations": "East Antarctica; Lake Vostok", "north": -75.5, "nsf_funding_programs": "Antarctic Earth Sciences", "paleo_time": null, "persons": "Bell, Robin; Studinger, Michael S.", "platforms": null, "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -79.0, "title": "Understanding the Boundary Conditions of the Lake Vostok Environment: A Site Survey for Future Work\r\n", "uid": "p0010097", "west": 101.0}, {"awards": "1142158 Cheng, Chi-Hing; 0231006 DeVries, Arthur", "bounds_geometry": "POLYGON((163 -76.5,163.5 -76.5,164 -76.5,164.5 -76.5,165 -76.5,165.5 -76.5,166 -76.5,166.5 -76.5,167 -76.5,167.5 -76.5,168 -76.5,168 -76.63,168 -76.76,168 -76.89,168 -77.02,168 -77.15,168 -77.28,168 -77.41,168 -77.54,168 -77.67,168 -77.8,167.5 -77.8,167 -77.8,166.5 -77.8,166 -77.8,165.5 -77.8,165 -77.8,164.5 -77.8,164 -77.8,163.5 -77.8,163 -77.8,163 -77.67,163 -77.54,163 -77.41,163 -77.28,163 -77.15,163 -77.02,163 -76.89,163 -76.76,163 -76.63,163 -76.5))", "dataset_titles": "High-resolution benthic seawater temperature record 1999-2012 (25-40m depth) from near intake jetty at McMurdo Station, Antarctica; Metadata associated with the description of Akarotaxis gouldae n. sp. (Bathydraconidae)", "datasets": [{"dataset_uid": "601811", "doi": null, "keywords": "Antarctica; Bellingshausen Sea; Cryosphere; Southern Ocean", "people": "Hilton, Eric; Desvignes, Thomas; Corso, Andrew; Cheng, Chi-Hing; Steinberg, Deborah; McDowell, Jan; Biesack, Ellen", "repository": "USAP-DC", "science_program": "LTER", "title": "Metadata associated with the description of Akarotaxis gouldae n. sp. (Bathydraconidae)", "url": "https://www.usap-dc.org/view/dataset/601811"}, {"dataset_uid": "601275", "doi": null, "keywords": "Antarctica; Benthic; McMurdo Sound; Mcmurdo Station; Oceans; Physical Oceanography; Temperature Probe; Water Temperature", "people": "Cheng, Chi-Hing; Devries, Arthur; Cziko, Paul", "repository": "USAP-DC", "science_program": null, "title": "High-resolution benthic seawater temperature record 1999-2012 (25-40m depth) from near intake jetty at McMurdo Station, Antarctica", "url": "https://www.usap-dc.org/view/dataset/601275"}], "date_created": "Wed, 08 Apr 2020 00:00:00 GMT", "description": "Antarctic notothenioid fishes exhibit two adaptive traits to survive in frigid temperatures. The first of these is the production of anti-freeze proteins in their blood and tissues. The second is a system-wide ability to perform cellular and physiological functions at extremely cold temperatures.The proposal goals are to show how Antarctic fishes use these characteristics to avoid freezing, and which additional genes are turned on, or suppressed in order for these fishes to maintain normal physiological function in extreme cold temperatures. Progressively colder habitats are encountered in the high latitude McMurdo Sound and Ross Shelf region, along with somewhat milder near?shore water environments in the Western Antarctic Peninsula (WAP). By quantifying the extent of ice crystals invading and lodging in the spleen, the percentage of McMurdo Sound fish during austral summer (Oct-Feb) will be compared to the WAP intertidal fish during austral winter (Jul-Sep) to demonstrate their capability and extent of freeze avoidance. Resistance to ice entry in surface epithelia (e.g. skin, gill and intestinal lining) is another expression of the adaptation of these fish to otherwise lethally freezing conditions. The adaptive nature of a uniquely characteristic polar genome will be explored by the study of the transcriptome (the set of expressed RNA transcripts that constitutes the precursor to set of proteins expressed by an entire genome). Three notothenioid species (E.maclovinus, D. Mawsoni and C. aceratus) will be analysed to document evolutionary genetic changes (both gain and loss) shaped by life under extreme chronic cold. A differential gene expression (DGE) study will be carried out on these different species to evaluate evolutionary modification of tissue-wide response to heat challenges. The transcriptomes and other sequencing libraries will contribute to de novo ice-fish genome sequencing efforts.", "east": 168.0, "geometry": "POINT(165.5 -77.15)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD", "is_usap_dc": true, "keywords": "McMurdo Sound; MARINE ECOSYSTEMS; Water Temperature; AQUATIC SCIENCES; OCEAN TEMPERATURE; FIELD INVESTIGATION; USAP-DC", "locations": "McMurdo Sound", "north": -76.5, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Cheng, Chi-Hing; Devries, Arthur", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -77.8, "title": "Antarctic Notothenioid Fish Freeze Avoidance and Genome-wide Evolution for Life in the Cold", "uid": "p0010091", "west": 163.0}, {"awards": "1341602 Crockett, Elizabeth; 1341663 O\u0027Brien, Kristin", "bounds_geometry": null, "dataset_titles": "Acclimation of cardiovascular function in Notothenia coriiceps; Adrenergic and adenosinergic regulation of the cardiovascular system in the Antarctic icefish Chaenocephalus aceratus; Cardiac metabolism in Antarctic fishes in response to an acute increase in temperature; Chaenocephalus aceratus HIF-1A mRNA, complete cds; Chionodraco rastrospinosus HIF-1A mRNA, partial cds; Effects of acute warming on cardiovascular performance of Antarctic fishes; Eleginops maclovinus HIF-1A mRNA, partial cds; Gymnodraco acuticeps HIF-1A mRNA, partial cds; Hypoxia response of hearts of Antarctic fishes; Maximum cardiac performance of Antarctic fishes that lack haemoglobin and myoglobin: exploring the effect of warming on nature\u2019s natural knockouts; Measurements of splenic contraction in Antarctic fishes; Mitochondrial membranes in cardiac muscle from Antarctic notothenioid fishes vary in phospholipid composition and membrane fluidity; Notothenia coriiceps HIF-1A mRNA, complete cds; Parachaenichthys charcoti HIF-1A mRNA, partial cds; Physical, chemical, and functional properties of neuronal membranes vary between species of Antarctic notothenioids differing in thermal tolerance; Thermal sensitivity of membrane fluidity and integrity in hearts of Antarctic fishes that vary in expression of hemoglobin and myoglobin", "datasets": [{"dataset_uid": "601408", "doi": "10.15784/601408", "keywords": "Antarctica; Antarctic Peninsula", "people": "Egginton, Stuart; Crockett, Elizabeth; O\u0027Brien, Kristin; Joyce, William; Axelsson, Michael; Farrell, Anthony", "repository": "USAP-DC", "science_program": null, "title": "Acclimation of cardiovascular function in Notothenia coriiceps", "url": "https://www.usap-dc.org/view/dataset/601408"}, {"dataset_uid": "601410", "doi": "10.15784/601410", "keywords": "Antarctica; Antarctic Peninsula; Biota; Fish", "people": "Crockett, Elizabeth; O\u0027Brien, Kristin; Joyce, William; Farrell, Anthony; Axelsson, Michael; Egginton, Stuart", "repository": "USAP-DC", "science_program": null, "title": "Effects of acute warming on cardiovascular performance of Antarctic fishes", "url": "https://www.usap-dc.org/view/dataset/601410"}, {"dataset_uid": "601409", "doi": "10.15784/601409", "keywords": "Antarctica; Antarctic Peninsula", "people": "O\u0027Brien, Kristin; Joyce, Michael; Axelsson, Michael; Farrell, Anthony; Egginton, Stuart", "repository": "USAP-DC", "science_program": null, "title": "Adrenergic and adenosinergic regulation of the cardiovascular system in the Antarctic icefish Chaenocephalus aceratus", "url": "https://www.usap-dc.org/view/dataset/601409"}, {"dataset_uid": "601414", "doi": "10.15784/601414", "keywords": "Antarctica; Antarctic Peninsula", "people": "Farnoud, Amir; Crockett, Elizabeth; O\u0027Brien, Kristin; Evans, Elizabeth", "repository": "USAP-DC", "science_program": null, "title": "Thermal sensitivity of membrane fluidity and integrity in hearts of Antarctic fishes that vary in expression of hemoglobin and myoglobin", "url": "https://www.usap-dc.org/view/dataset/601414"}, {"dataset_uid": "601407", "doi": "10.15784/601407", "keywords": "Antarctica; Antarctic Peninsula", "people": "Joyce, William; Axelsson, Michael; O\u0027Brien, Kristin", "repository": "USAP-DC", "science_program": null, "title": "Measurements of splenic contraction in Antarctic fishes", "url": "https://www.usap-dc.org/view/dataset/601407"}, {"dataset_uid": "601406", "doi": "10.15784/601406", "keywords": "Antarctica; Antarctic Peninsula", "people": "O\u0027Brien, Kristin", "repository": "USAP-DC", "science_program": null, "title": "Hypoxia response of hearts of Antarctic fishes", "url": "https://www.usap-dc.org/view/dataset/601406"}, {"dataset_uid": "601405", "doi": "10.15784/601405", "keywords": "Antarctica; Antarctic Peninsula", "people": "O\u0027Brien, Kristin", "repository": "USAP-DC", "science_program": null, "title": "Cardiac metabolism in Antarctic fishes in response to an acute increase in temperature", "url": "https://www.usap-dc.org/view/dataset/601405"}, {"dataset_uid": "200184", "doi": "10.5061/dryad.83vc5", "keywords": null, "people": null, "repository": "Dryad", "science_program": null, "title": "Maximum cardiac performance of Antarctic fishes that lack haemoglobin and myoglobin: exploring the effect of warming on nature\u2019s natural knockouts", "url": "https://doi.org/10.5061/dryad.83vc5"}, {"dataset_uid": "200185", "doi": "10.5061/dryad.k90h35k", "keywords": null, "people": null, "repository": "Dryad", "science_program": null, "title": "Mitochondrial membranes in cardiac muscle from Antarctic notothenioid fishes vary in phospholipid composition and membrane fluidity", "url": "https://doi.org/10.5061/dryad.k90h35k"}, {"dataset_uid": "200186", "doi": "10.5061/dryad.qm0b25h", "keywords": null, "people": null, "repository": "Dryad", "science_program": null, "title": "Physical, chemical, and functional properties of neuronal membranes vary between species of Antarctic notothenioids differing in thermal tolerance", "url": "https://doi.org/10.5061/dryad.qm0b25h"}, {"dataset_uid": "200187", "doi": null, "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "Chaenocephalus aceratus HIF-1A mRNA, complete cds", "url": "https://www.ncbi.nlm.nih.gov/nuccore/KX950828"}, {"dataset_uid": "200188", "doi": null, "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "Notothenia coriiceps HIF-1A mRNA, complete cds", "url": "https://www.ncbi.nlm.nih.gov/nuccore/KX950829"}, {"dataset_uid": "200189", "doi": null, "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "Eleginops maclovinus HIF-1A mRNA, partial cds", "url": "https://www.ncbi.nlm.nih.gov/nuccore/KX950830"}, {"dataset_uid": "200190", "doi": null, "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "Parachaenichthys charcoti HIF-1A mRNA, partial cds", "url": "https://www.ncbi.nlm.nih.gov/nuccore/KX950833"}, {"dataset_uid": "200191", "doi": null, "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "Gymnodraco acuticeps HIF-1A mRNA, partial cds", "url": "https://www.ncbi.nlm.nih.gov/nuccore/kx950832"}, {"dataset_uid": "200192", "doi": null, "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "Chionodraco rastrospinosus HIF-1A mRNA, partial cds", "url": "https://www.ncbi.nlm.nih.gov/nuccore/kx950831"}], "date_created": "Wed, 26 Feb 2020 00:00:00 GMT", "description": "The ocean surrounding Antarctica is home to an extraordinary assemblage of fishes, dominated by a single group that are extremely well-suited to life in icy waters and which are of significant ecological importance there. Of great concern is the capacity of these fishes to withstand increases in temperature as the region of the Western Antarctic Peninsula warms at a rate faster than any other area in the Southern hemisphere. One particular group of Antarctic fishes, known as the icefishes, are particularly vulnerable to increases in temperature because unlike all other vertebrates on earth, icefishes are white-blooded due to their lack of the oxygen-binding protein hemoglobin. This greatly reduces their capacity to transport and deliver oxygen to tissues compared to red-blooded Antarctic fishes. Previous studies have shown that icefishes are indeed less tolerant to elevations in temperature but the underlying factors are completely unknown. Additionally, it is not understood if red- or white-blooded Antarctic fishes can adjust, or acclimate, to modest increases in temperature, similar to those changes in temperature the animals might experience as the earth warms. The investigators will determine if heart function and/or nervous system function limits thermal tolerance of Antarctic fishes, and will determine their capacity to acclimate to warmer temperatures. The project will further the NSF goal of training new generations of scientists by training graduate and undergraduate students. In addition, the project will collaborate with a high school biology teacher from a school which serves a largely minority student body. The students will learn about the marine environment, and will construct a camera to be used in the field to learn more about Antarctic fishes. Two students and the teacher will also attend a summer marine biology internship program. Antarctic fishes within the suborder Notothenioidei (called \"notothenioids\") are among the organisms on earth least able to deal with changes in temperature. The hemoglobinless icefish are even less able to withstand temperature changes than are red-blooded notothenioids. While this is well documented, the underlying physiological and biochemical mechanisms responsible are unknown. The investigators will test the hypotheses that cardiac work is significantly greater in icefishes compared to red-blooded species, and that as temperature increases, the greater cardiac work of icefishes, coupled with reduced blood oxygen-carrying capacity, results in cardiac failure at a lower temperature compared to red-blooded species. They also hypothesize that neuronal function limits thermal tolerance of red-blooded notothenioids. These hypotheses will be tested using a wide variety of experiments. For example, the investigators will measure heart rate concurrently with critical thermal maximum. They will also characterize metabolic and gene-expression responses to elevated temperature and determine if mitochondrial function contributes to thermal tolerance using a variety of techniques. To determine if neuronal function limits thermal tolerance they will quantify behavioral responses to warming of whole animals and to warming of only the brain area. They will also determine if acclimation to warmer temperatures impacts heart function and they will measure activities of a variety of enzymes from central metabolic pathways.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "Antarctica; Amd/Us; FISH; USA/NSF; FIELD INVESTIGATION; AMD; Antarctic Peninsula; LABORATORY; USAP-DC", "locations": "Antarctica; Antarctic Peninsula", "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Crockett, Elizabeth; O\u0027Brien, Kristin", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION; OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repo": "USAP-DC", "repositories": "Dryad; GenBank; USAP-DC", "science_programs": null, "south": null, "title": "Collaborative Research: The Physiological and Biochemical Underpinnings of Thermal Tolerance in Antarctic Notothenioid Fishes", "uid": "p0010084", "west": null}, {"awards": "1543383 Postlethwait, John", "bounds_geometry": "POLYGON((-66 -62,-65.2 -62,-64.4 -62,-63.6 -62,-62.8 -62,-62 -62,-61.2 -62,-60.4 -62,-59.6 -62,-58.8 -62,-58 -62,-58 -62.4,-58 -62.8,-58 -63.2,-58 -63.6,-58 -64,-58 -64.4,-58 -64.8,-58 -65.2,-58 -65.6,-58 -66,-58.8 -66,-59.6 -66,-60.4 -66,-61.2 -66,-62 -66,-62.8 -66,-63.6 -66,-64.4 -66,-65.2 -66,-66 -66,-66 -65.6,-66 -65.2,-66 -64.8,-66 -64.4,-66 -64,-66 -63.6,-66 -63.2,-66 -62.8,-66 -62.4,-66 -62))", "dataset_titles": "C. aceratus pronephric kidney (head kidney) miRNA; mirtop\r\ncommand lines tool to annotate miRNAs with a standard mirna/isomir naming; Patagonotothen cornucola isolate Pcor_18_01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial; Patagonotothen sima isolate Psim_18_11 cardiac muscle myosin heavy chain 6 (myh6) gene, partial cds; Patagonotothen sima isolate Psim_18_11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial; Patagonotothen sima isolate Psim_18_12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial; Prost!, a tool for miRNA annotation and next generation smallRNA sequencing experiment analysis; Quantifying expression levels of smallRNAs between tissues in Danio Rerio strain AB.; Quantifying expression levels of smallRNAs between tissues in three-spined stickleback", "datasets": [{"dataset_uid": "200132", "doi": "", "keywords": null, "people": null, "repository": "GitHub", "science_program": null, "title": "mirtop\r\ncommand lines tool to annotate miRNAs with a standard mirna/isomir naming", "url": "https://github.com/miRTop"}, {"dataset_uid": "200131", "doi": "", "keywords": null, "people": null, "repository": "GitHub", "science_program": null, "title": "Prost!, a tool for miRNA annotation and next generation smallRNA sequencing experiment analysis", "url": "https://github.com/uoregon-postlethwait/prost"}, {"dataset_uid": "200128", "doi": "", "keywords": null, "people": null, "repository": "NCBI SRA", "science_program": null, "title": "Quantifying expression levels of smallRNAs between tissues in three-spined stickleback", "url": "https://www.ncbi.nlm.nih.gov/search/all/?term=SRP157992"}, {"dataset_uid": "200130", "doi": "", "keywords": null, "people": null, "repository": "NCBI SRA", "science_program": null, "title": "C. aceratus pronephric kidney (head kidney) miRNA", "url": "https://www.ncbi.nlm.nih.gov/search/all/?term=SRP069031"}, {"dataset_uid": "200129", "doi": "", "keywords": null, "people": null, "repository": "NCBI SRA", "science_program": null, "title": "Quantifying expression levels of smallRNAs between tissues in Danio Rerio strain AB.", "url": "https://www.ncbi.nlm.nih.gov/search/all/?term=SRP039502"}, {"dataset_uid": "200135", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Patagonotothen sima isolate Psim_18_12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial", "url": "https://www.ncbi.nlm.nih.gov/search/all/?term=MN136233+"}, {"dataset_uid": "200136", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Patagonotothen sima isolate Psim_18_11 cardiac muscle myosin heavy chain 6 (myh6) gene, partial cds", "url": "https://www.ncbi.nlm.nih.gov/search/all/?term=MN136234+"}, {"dataset_uid": "200134", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Patagonotothen sima isolate Psim_18_11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial", "url": "https://www.ncbi.nlm.nih.gov/search/all/?term=MN136232"}, {"dataset_uid": "200133", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Patagonotothen cornucola isolate Pcor_18_01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial", "url": "https://www.ncbi.nlm.nih.gov/search/all/?term=MN136231"}], "date_created": "Wed, 26 Feb 2020 00:00:00 GMT", "description": "Icefish live in frigid Antarctic seas, and have unique traits such as the absence of red blood cells, enlarged hearts, large diameter blood vessels, low bone mineral densities, and fat droplets that disrupt their muscles. These features would be harmful in other animals. In mammals and fish inhabiting warm waters, development of organs involved in these traits is modulated by genes that encode specific proteins, but the rate of protein production is often regulated by short RNA molecules called microRNAs (miRNAs). Genes that code for proteins must first make an RNA copy, and the actual protein is made from this RNA copy intermediate. MiRNAs regulate the amount of protein that is made by binding to the RNA intermediate and interrupting its production of protein. Binding of miRNAs to RNA depends strongly on temperature. Regulation of genes by miRNAs has not been studied in Antarctic fish, which live in seas with temperatures below the freezing point of fresh water. This project will compare miRNA regulation 1) in Antarctic fish vs. warm-water fish to learn how miRNAs regulate gene expression in constant cold; and 2) in Antarctic icefish with no red blood cells, enlarged hearts, and reduced bone density vs. closely related Antarctic fish containing red blood cells, normal hearts, and dense bones. The project will have broad impacts to science and society nationally and globally. First, this will be the first study of important factors in gene regulation (miRNAs) in Antarctic fish, which are an essential component of the entire ecology of the Southern Ocean, and will shed light on how these fish might respond to the warming of Antarctic waters. Second, it will bring Antarctic science to under-represented high school students at a local alternative downtown high school by conducting video conferences during the Antarctic field seasons and hosting student investigations of Antarctic fish in the research laboratory. microRNAs (miRNAs) are key post-transcriptional regulators of gene expression that modulate development and physiology in temperate animals. Although miRNAs act by binding to messenger RNAs (mRNAs), a process that is strongly sensitive to temperature, miRNAs have yet not been studied in Antarctic animals, including Notothenioid fish, which dominate the Southern Ocean. This project will compare miRNA regulation in 1) Antarctic vs. temperate fish to learn the roles of miRNA regulation in adaptation to constant cold; and in 2) bottom-dwelling, dense-boned, red-blooded Nototheniods vs. high buoyancy, osteopenic, white-blooded icefish to understand miRNA regulation in specialized organs after the evolution of the loss of hemoglobin genes and red blood cells, the origin of enlarged heart and vasculature, and the evolution of increased buoyancy, which arose by decreased bone mineralization and increased lipid deposition. Aim 1 is to test the hypothesis that Antarctic fish evolved miRNA-related genome specializations in response to constant cold. The project will compare four Antarctic Notothenioid species to two temperate Notothenioids and two temperate laboratory species to test the hypotheses that (a) Antarctic fish evolved miRNA genome repertoires by loss of ancestral genes and/or gain of new genes, (b) express miRNAs that are involved in cold tolerance, and (c) respond to temperature change by changing miRNA gene expression. Aim 2 is to test the hypothesis that the evolution of icefish from red-blooded bottom-dwelling ancestors was accompanied by an altered miRNA genomic repertoire, sequence, and/or expression. The project will test the hypotheses that (a) miRNAs in icefish evolved in sequence and/or in expression in icefish specializations, including head kidney (origin of red blood cells); heart (changes in vascular system), cranium and pectoral girdle (reduced bone mineral density); and skeletal muscle (lipid deposition), and (b) miRNAs that evolved in icefish specializations had ancestral functions related to their derived roles in icefish, as determined by functional tests of zebrafish orthologs of icefish miRNAs in developing zebrafish. The program will isolate, sequence, and determine the expression of miRNAs and mRNAs using high-throughput transcriptomics and novel software. Results will show how the microRNA system evolves in vertebrate animals pushed to physiological extremes and provide insights into the prospects of key species in the most rapidly warming part of the globe.", "east": -58.0, "geometry": "POINT(-62 -64)", "instruments": null, "is_usap_dc": true, "keywords": "USAP-DC; Palmer Station; NOT APPLICABLE; FISH", "locations": "Palmer Station", "north": -62.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Postlethwait, John; Desvignes, Thomas", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "GitHub", "repositories": "GitHub; NCBI GenBank; NCBI SRA", "science_programs": null, "south": -66.0, "title": "Antarctic Fish and MicroRNA Control of Development and Physiology", "uid": "p0010085", "west": -66.0}, {"awards": "1341393 Denlinger, David; 1341385 Lee, Richard", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "Alaskozetes antarcticus Raw sequence reads; Belgica antarctica Integrated Genome and Transcriptome Project; Data from: Rapid cold hardening protects against sublethal freezing injury in an Antarctic insect", "datasets": [{"dataset_uid": "200053", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Belgica antarctica Integrated Genome and Transcriptome Project", "url": "https://www.ncbi.nlm.nih.gov/bioproject/175916"}, {"dataset_uid": "200052", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Alaskozetes antarcticus Raw sequence reads", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA428758"}, {"dataset_uid": "200054", "doi": " https://doi.org/10.5061/dryad.29p7ng2", "keywords": null, "people": null, "repository": "Dryad", "science_program": null, "title": "Data from: Rapid cold hardening protects against sublethal freezing injury in an Antarctic insect", "url": "https://datadryad.org/resource/doi:10.5061/dryad.29p7ng2"}], "date_created": "Mon, 12 Aug 2019 00:00:00 GMT", "description": "Polar regions are deserts that are not only cold but also lack access to free water. Antarctic insects have unique survival mechanisms including the ability to tolerate freezing and extensive dehydration, surviving the loss of 70% of their body water. How this is done is of interest not only for understanding seasonal adaptations of insects and how they respond to climate change, but the molecular and physiological mechanisms employed may offer valuable insights into more general mechanisms that might be exploited for cryopreservation and long-term storage of human tissues and organs for transplantation and other medical applications. The investigators will study the proteins that are responsible for removing water from the body, cell level consequences of this, and how the responsible genes vary between populations. The project will also further the NSF goals of making scientific discoveries available to the general public and of training new generations of scientists. Each year a K-12 teacher will be a member of the field team and assist with fieldwork and outreach to school children and their teachers. Educational outreach efforts include presentations at local schools and national teacher meetings, providing lesson plans and podcasts on a website, and continuing to publish articles related to this research in education journals. In addition, undergraduate and graduate students will receive extensive training in all aspects of the research project with extended experiences that include publication of scientific papers and presentations at national meetings. This project focuses on deciphering the physiological and molecular mechanisms that enable the Antarctic midge Belgica antarctica to survive environmental stress and the loss of most of its body water in the desiccating polar environment. This extremophile is an ideal system for investigating mechanisms of stress tolerance and local geographic adaptations and its genome has recently been sequenced. This project has three focal areas: 1) Evaluating the role of aquaporins (water channel proteins) in the rapid removal of water from the body by studying expression of their genes during dehydration; 2) Investigating the mechanism of metabolic depression and the role of autophagy (controlled breakdown of cellular components) as a mediator of stress tolerance by studying expression of the genes responsible for autophagy during the dehydration process; and 3) Evaluating the population structure, gene flow, and adaptive variation in physiological traits associated with stress tolerance using a genetic approach that takes advantage of the genomic sequence available for this species coupled with physiological and environmental data from the sampled populations and their habitats.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "Antarctica; USAP-DC; ARTHROPODS; NOT APPLICABLE", "locations": "Antarctica", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Denlinger, David; Lee, Richard", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "NCBI GenBank", "repositories": "Dryad; NCBI GenBank", "science_programs": null, "south": -90.0, "title": "Collaborative Research: Winter Survival Mechanisms and Adaptive Genetic Variation in an Antarctic Insect", "uid": "p0010048", "west": -180.0}, {"awards": "1745036 Marchetti, Adrian; 1744760 Hopkinson, Brian", "bounds_geometry": "POLYGON((-77 -61,-75.2 -61,-73.4 -61,-71.6 -61,-69.8 -61,-68 -61,-66.2 -61,-64.4 -61,-62.6 -61,-60.8 -61,-59 -61,-59 -62.1,-59 -63.2,-59 -64.3,-59 -65.4,-59 -66.5,-59 -67.6,-59 -68.7,-59 -69.8,-59 -70.9,-59 -72,-60.8 -72,-62.6 -72,-64.4 -72,-66.2 -72,-68 -72,-69.8 -72,-71.6 -72,-73.4 -72,-75.2 -72,-77 -72,-77 -70.9,-77 -69.8,-77 -68.7,-77 -67.6,-77 -66.5,-77 -65.4,-77 -64.3,-77 -63.2,-77 -62.1,-77 -61))", "dataset_titles": "Photosynthetic physiological data of Proteorhodopsin containing diatoms under differing iron availabilities", "datasets": [{"dataset_uid": "601530", "doi": "10.15784/601530", "keywords": "Antarctica; Diatom", "people": "Plumb, Kaylie; Hopkinson, Brian; Andrew, Sarah; Marchetti, Adrian", "repository": "USAP-DC", "science_program": null, "title": "Photosynthetic physiological data of Proteorhodopsin containing diatoms under differing iron availabilities", "url": "https://www.usap-dc.org/view/dataset/601530"}], "date_created": "Sun, 16 Jun 2019 00:00:00 GMT", "description": "Proteorhodopsins are proteins that are embedded in membranes that can act as light-driven proton pumps to generate energy for metabolism and growth. The discovery of proteorhodopsins in many diverse marine prokaryotic microbes has initiated extensive investigation into their distributions and functional roles. Recently, a proton-pumping, rhodopsin-like gene was identified in diatoms, a group of marine phytoplankton that dominates the base of the food web in much of the Southern Ocean. Since this time, proteorhodopsins have been identified in many, but not all, diatom species. The proteorhodopsin gene is more frequently found in diatoms residing in cold, iron-limited regions of the ocean, including the Southern Ocean, than in diatoms from other regions. It is thought that proteorhodopsin is especially suited for use energy production in the Southern Ocean since it uses no iron and its reaction rate is insensitive to temperature (unlike conventional photosynthesis). The overall objective of the project is to characterize Antarctic diatom-proteorhodopsin and determine its role in the adaptation of these diatoms to low iron concentrations and extremely low temperatures found in Antarctic waters. This research will provide new information on the genetic underpinnings that contribute to the success of diatoms in the Southern Ocean and how this unique molecule may play a pivotal role in providing energy to the base of the Antarctic food web. Broader impact activities are aimed to promote the teaching and learning of polar marine-sciences related topics by translating research objectives into readily accessible educational materials for middle-school students. This project will combine molecular, biochemical and physiological measurements to determine the role and importance of proteorhodopsin in diatom isolates from the Western Antarctic Peninsula region. Proton-pumping characteristics and pumping rates of proteorhodopsin as a function of light intensity and temperature, the resultant proteorhodopsin-linked intracellular ATP production rates, and the cellular localization of the protein will be determined. The project will examine the environmental conditions where Antarctic diatom-proteorhodopsin is most highly expressed and construct a cellular energy budget that includes diatom-proteorhodopsin when grown under these different environmental conditions. Estimates of the energy flux generated by proteorhodopsin will be compared to total energy generation by the photosynthetic light reactions and metabolically coupled respiration rates. Finally, the characteristics and gene expression of diatom-proteorhodopsin in Antarctic diatoms and a proteorhodopsin-containing diatom isolates from temperate regions will be compared in order to determine if there is a preferential dependence on energy production through proteorhodopsin in diatoms residing in cold, iron-limited regions of the ocean. Educational activities will be performed in collaboration with the Morehead Planetarium and Science Center who co-ordinates the SciVentures program, a popular summer camp for middle-school students from Chapel Hill and surrounding areas. In collaboration with the Planetarium, the researchers will develop activities that focus on phytoplankton and the important role they play within polar marine food webs for the SciVentures participants. Additionally, a teaching module on Antarctic phytoplankton will be developed for classrooms and made available to educational networking websites and presented at workshops for science educators nationwide. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": -59.0, "geometry": "POINT(-68 -66.5)", "instruments": null, "is_usap_dc": true, "keywords": "FIELD INVESTIGATION; NSF/USA; Southern Ocean; AMD; Amd/Us; LABORATORY; USAP-DC; BIOGEOCHEMICAL CYCLES", "locations": "Southern Ocean", "north": -61.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Marchetti, Adrian; Septer, Alecia; Hopkinson, Brian", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION; OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -72.0, "title": "Collaborative research: Antarctic diatom proteorhodopsins: Characterization and a potential role in the iron-limitation response", "uid": "p0010033", "west": -77.0}, {"awards": "1247510 Detrich, H. William", "bounds_geometry": null, "dataset_titles": "E-MTAB-6759: RNA-seq across tissues in four Notothenioid species (Antarctic icefish); Expedition Data; Expedition data of LMG1003; Expedition data of LMG1004; PRJNA420419: Chaenocephalus aceratus Genome sequencing; PRJNA66471: Notothenia coriiceps Genome Sequencing Notothenia coriiceps isolate:Sejong01 (black rockcod); S-BSST 132: Assembled Transcriptomes for Berthelot et al. (2018); SRA091269: Notothenia coriiceps RNA Raw Sequence Reads; SRP047484: RAD-tag Sequences of Genetically Mapped Notothenia coriiceps Embryos ; SRP118539: RAD-tag Sequences of Genetically Mapped Chaenocephalus aceratus Embryos", "datasets": [{"dataset_uid": "200146", "doi": "", "keywords": null, "people": null, "repository": "NCBI SRA", "science_program": null, "title": "SRP047484: RAD-tag Sequences of Genetically Mapped Notothenia coriiceps Embryos ", "url": "https://www.ncbi.nlm.nih.gov/sra/?term=SRP047484"}, {"dataset_uid": "200144", "doi": "", "keywords": null, "people": null, "repository": "Array Express", "science_program": null, "title": "E-MTAB-6759: RNA-seq across tissues in four Notothenioid species (Antarctic icefish)", "url": "https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-6759/"}, {"dataset_uid": "001509", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0806"}, {"dataset_uid": "200145", "doi": "", "keywords": null, "people": null, "repository": "BioStudies", "science_program": null, "title": "S-BSST 132: Assembled Transcriptomes for Berthelot et al. (2018)", "url": "https://www.ebi.ac.uk/biostudies/studies/S-BSST132"}, {"dataset_uid": "200026", "doi": "", "keywords": null, "people": null, "repository": "NCBI SRA", "science_program": null, "title": "SRA091269: Notothenia coriiceps RNA Raw Sequence Reads", "url": "https://www.ncbi.nlm.nih.gov/sra/?term=SRA091269"}, {"dataset_uid": "200142", "doi": "", "keywords": null, "people": null, "repository": "NCBI BioProject", "science_program": null, "title": "PRJNA66471: Notothenia coriiceps Genome Sequencing Notothenia coriiceps isolate:Sejong01 (black rockcod)", "url": "https://www.ncbi.nlm.nih.gov/bioproject/66471"}, {"dataset_uid": "200143", "doi": "", "keywords": null, "people": null, "repository": "NCBI BioProject", "science_program": null, "title": "PRJNA420419: Chaenocephalus aceratus Genome sequencing", "url": "https://www.ncbi.nlm.nih.gov/bioproject/420419"}, {"dataset_uid": "001508", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0807"}, {"dataset_uid": "002685", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG1004", "url": "https://www.rvdata.us/search/cruise/LMG1004"}, {"dataset_uid": "002684", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG1003", "url": "https://www.rvdata.us/search/cruise/LMG1003"}, {"dataset_uid": "200093", "doi": "", "keywords": null, "people": null, "repository": "NCBI SRA", "science_program": null, "title": "SRP118539: RAD-tag Sequences of Genetically Mapped Chaenocephalus aceratus Embryos", "url": "https://www.ncbi.nlm.nih.gov/sra/SRP118539 "}], "date_created": "Mon, 08 Apr 2019 00:00:00 GMT", "description": "Since the advent of Antarctic continental glaciation, the opening of the Drake Passage between South America and the Antarctic Peninsula, and the onset of cooling of the Southern Ocean ~40-25 million years ago, evolution of the Antarctic marine biota has been driven by the development of extreme cold temperatures. As circum-Antarctic coastal temperatures declined during this period from ~20\u00b0C to the modern ?1.9 to +2.0\u00b0C (reached ~8-10 million years ago), the psychrophilic (cold-loving) ectotherms of the Southern Ocean evolved compensatory molecular, cellular, and physiological traits that enabled them to maintain normal metabolic function at cold temperatures. Today, these organisms are threatened by rapid warming of the Southern Ocean over periods measured in centuries (as much as 5\u00b0C/100 yr), a timeframe so short that re-adaptation and/or acclimatization to the ?new warm? may not be possible. Thus, the long-term goals of this research project are: 1) to understand the biochemical and physiological capacities of the embryos of Antarctic notothenioid fish to resist or compensate for rapid oceanic warming; and 2) to assess the genetic toolkit available to support the acclimatization and adaptation of Antarctic notothenioid embryos to their warming habitat. The specific aims of this work are: 1) to determine the capacity of the chaperonin complex of notothenioid fishes to assist protein folding at temperatures between ?4 and +20\u00b0C; and 2) to evaluate the genetic responses of notothenioid embryos, measured as global differential gene transcription, to temperature challenge, with ?1.9\u00b0C as the ?normal? control and +4 and +10\u00b0C as high temperature insults. The physiology of embryonic development of marine stenotherms under future climate change scenarios is an important but understudied problem. This project will provide valuable insights into the capacity of Antarctic fish embryos to acclimatize and adapt to plausible climate change scenarios by examining multiple levels of biological organization, from the biochemical to the organismal. The results should also be broadly applicable to understanding the impact of global warming on marine biota worldwide. The research will also introduce graduate and REU undergraduate students to state-of-the-art biochemical, cellular, and molecular-biological research relevant to ecological and environmental issues of the Antarctic marine ecosystem.", "east": null, "geometry": null, "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e SAMPLERS \u003e TRAWLS/NETS \u003e BOTTOM TRAWL", "is_usap_dc": false, "keywords": "AQUATIC SCIENCES; R/V LMG; USAP-DC; Southern Ocean", "locations": "Southern Ocean", "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Detrich, H. William", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "NCBI SRA", "repositories": "Array Express; BioStudies; NCBI BioProject; NCBI SRA; R2R", "science_programs": null, "south": null, "title": "Protein Folding and Embryogenesis in Antarctic Fishes: A Comparative Approach to Environmental Stress", "uid": "p0010024", "west": null}, {"awards": "1246407 Jenouvrier, Stephanie", "bounds_geometry": "POINT(70.2433 -49.6875)", "dataset_titles": "Demographic outputs and their variances for three life history complexes for the Southern Fulmar across contrasted sea ice conditions.; Desprez, M., Jenouvrier, S., Barbraud, C., Delord, K. and Weimerskirch, H., 2018. Linking oceanographic conditions, migratory schedules and foraging behaviour during the non\u2010breeding season to reproductive performance in a long\u2010lived seabird. Functional ecology, 32(8), pp.2040-2053.; Jenouvrier, S., Desprez, M., Fay, R., Barbraud, C., Weimerskirch, H., Delord, K. and Caswell, H., 2018. Climate change and functional traits affect population dynamics of a long\u2010lived seabird. Journal of Animal Ecology, 87(4), pp.906-920.; Linking oceanographic conditions, migratory schedules and foraging behaviour during the non-breeding season to reproductive performance in a long-lived seabird", "datasets": [{"dataset_uid": "200007", "doi": "10.1111/1365-2656.12827.", "keywords": null, "people": null, "repository": "Dryad", "science_program": null, "title": "Jenouvrier, S., Desprez, M., Fay, R., Barbraud, C., Weimerskirch, H., Delord, K. and Caswell, H., 2018. Climate change and functional traits affect population dynamics of a long\u2010lived seabird. Journal of Animal Ecology, 87(4), pp.906-920.", "url": "https://doi.org/10.5061/dryad.h5vk5"}, {"dataset_uid": "601585", "doi": "10.15784/601585", "keywords": "Antarctica; Biota; Birds; East Antarctica; Southern Fulmar", "people": "Jenouvrier, Stephanie", "repository": "USAP-DC", "science_program": null, "title": "Demographic outputs and their variances for three life history complexes for the Southern Fulmar across contrasted sea ice conditions.", "url": "https://www.usap-dc.org/view/dataset/601585"}, {"dataset_uid": "601140", "doi": "10.15784/601140", "keywords": "Albatross; Animal Behavior Observation; Antarctica; Biota; Birds; Black-Browed Albatross (thalassarche Melanophris); Field Investigations; Foraging; Kerguelen Island; Ocean Island/plateau; Ocean Island/Plateau; Southern Ocean", "people": "Jenouvrier, Stephanie", "repository": "USAP-DC", "science_program": null, "title": "Linking oceanographic conditions, migratory schedules and foraging behaviour during the non-breeding season to reproductive performance in a long-lived seabird", "url": "https://www.usap-dc.org/view/dataset/601140"}, {"dataset_uid": "200008", "doi": "10.1111/1365-2435.13117", "keywords": null, "people": null, "repository": "Dryad", "science_program": null, "title": "Desprez, M., Jenouvrier, S., Barbraud, C., Delord, K. and Weimerskirch, H., 2018. Linking oceanographic conditions, migratory schedules and foraging behaviour during the non\u2010breeding season to reproductive performance in a long\u2010lived seabird. Functional ecology, 32(8), pp.2040-2053.", "url": "https://datadryad.org/resource/doi:10.5061/dryad.pb209db"}], "date_created": "Thu, 31 Jan 2019 00:00:00 GMT", "description": "Understanding the ecological consequences - present and future-of climate change is a central question in conservation biology. The goal of this project is to identify the effects of climate change on the Black-Browed Albatross, a seabird breeding in the Southern Ocean. The Black-Browed Albatross exhibits remarkable flight adaptations, using winds as an energy source to glide for long distances. This is the basis of their foraging strategy, by which they obtain food for themselves and their offspring. Climate change, however, is expected to modify wind patterns over the Southern Ocean. This project will analyze the effect of winds on life history traits (foraging behaviors, body conditions and demographic traits), and the effects of these traits on populations. New demographic models will provide the link between foraging behavior and the physical environment, and evaluate the persistence of this population in the face of climate change. Understanding and predicting population responses to climate change is important because the world?s climate will continue to change throughout the 21st century and beyond. To help guide conservation strategies and policy decisions in the face of climate change, reliable assessments of population extinction risks are urgently needed. The Black-Browed Albatross is considered endangered by the International Union for Conservation of Nature due to recent drastic reductions in its population size. This project will improve our understanding of the mechanisms by which climate affects the life history and populations of Black-Browed Albatross to improve prediction of extinction risks under future climate change.", "east": 70.2433, "geometry": "POINT(70.2433 -49.6875)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "Southern Ocean; NOT APPLICABLE; USAP-DC; BIRDS", "locations": "Southern Ocean", "north": -49.6875, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Jenouvrier, Stephanie", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "Dryad", "repositories": "Dryad; USAP-DC", "science_programs": null, "south": -49.6875, "title": "Linking Foraging Behaviors to Demography to understand Albatrosses Population Responses to Climate Change", "uid": "p0010002", "west": 70.2433}, {"awards": "1341476 Moran, Amy", "bounds_geometry": "POINT(166.666 -77.84999)", "dataset_titles": "Cuticle morphology and oxygen gradients of Antarctic sea spiders; Physiological and biochemical measurements on Pycnogonida from McMurdo Sound; Physiological, biomechanical, and locomotory data on Antarctic sea spiders fouled and unfouled with epibionts; Size scaling of oxygen physiology and metabolic rate of Antarctic sea spiders", "datasets": [{"dataset_uid": "601145", "doi": "10.15784/601145", "keywords": "Antarctica; Benthos; Biota; Body Size; Cuticle; McMurdo Sound; Microelectrodes; Microscope; Microscopy; Oxygen; Pore; Respiration; Sea Spider; Southern Ocean", "people": "Woods, H. Arthur; Arthur Woods, H.", "repository": "USAP-DC", "science_program": null, "title": "Cuticle morphology and oxygen gradients of Antarctic sea spiders", "url": "https://www.usap-dc.org/view/dataset/601145"}, {"dataset_uid": "601142", "doi": "10.15784/601142", "keywords": "Antarctica; Biomechanics; Biota; Cold Adaptation; McMurdo Sound; Metabolism; Oceans; Oxygen; Pycnogonida; Southern Ocean", "people": "Moran, Amy; Woods, H. Arthur; Tobalske, Bret", "repository": "USAP-DC", "science_program": null, "title": "Physiological and biochemical measurements on Pycnogonida from McMurdo Sound", "url": "https://www.usap-dc.org/view/dataset/601142"}, {"dataset_uid": "601149", "doi": "10.15784/601149", "keywords": "Antarctica; Barnacles; Biota; Cuticle; Epibionts; Fouling; Grooming; Locomotion; Oxygen; Respiration", "people": "Moran, Amy; Shishido, Caitlin; Woods, H. Arthur; Lane, Steven J.; Tobalske, Bret", "repository": "USAP-DC", "science_program": null, "title": "Physiological, biomechanical, and locomotory data on Antarctic sea spiders fouled and unfouled with epibionts", "url": "https://www.usap-dc.org/view/dataset/601149"}, {"dataset_uid": "601150", "doi": "10.15784/601150", "keywords": "Antarctica; Biota; Body Size; Cuticle; Metabolic Rate; Oxygen; Polar Gigantism; Respiration; Size Limits; Southern Ocean; Temperature", "people": "Lane, Steven J.; Moran, Amy; Shishido, Caitlin; Woods, H. Arthur", "repository": "USAP-DC", "science_program": null, "title": "Size scaling of oxygen physiology and metabolic rate of Antarctic sea spiders", "url": "https://www.usap-dc.org/view/dataset/601150"}], "date_created": "Mon, 10 Dec 2018 00:00:00 GMT", "description": "Beginning with the earliest expeditions to the poles, scientists have noted that many polar taxa grow to unusually large body sizes, a phenomenon now known as \u0027polar gigantism.\u0027 Although scientists have been interested in polar giants for many years, many questions still remain about the biology of this significant form of polar diversity. This award from the Antarctic Organisms and Ecosystems program within the Polar Sciences Division at the National Science Foundation will investigate the respiratory and biomechanical mechanisms underlying polar gigantism in Antarctic pycnogonids (commonly known as sea spiders). The project will use a series of manipulative experiments to investigate the effects of temperature and oxygen availability on respiratory capacity and biomechanical strength, and will compare Antarctic sea spiders to related species from temperate and tropical regions. The research will provide insight into the ability of polar giants to withstand the warming polar ocean temperatures associated with climate change.\u003cbr/\u003e\u003cbr/\u003eThe prevailing hypothesis to explain the evolution of gigantism invokes shifts in respiratory relationships in extremely cold ocean waters: in the cold, oxygen is more plentiful while at the same time metabolic rates are very low. Together these effects alleviate constraints on oxygen supply that restrict organisms living in warmer waters. Respiratory capacity must evolve in the context of adaptive tradeoffs, so for organisms including pycnogonids there must be tradeoffs between respiratory capacity and resistance to biomechanical stresses. The investigators will test a novel hypothesis that respiratory challenges are not associated with particular body sizes, and will answer the following questions: What are the dynamics of oxygen transport and consumption in Antarctic pycnogonids; how do structural features related to oxygen diffusion trade off with requirements for body support and locomotion; how does body size influence vulnerability to environmental hypoxia and to temperature-oxygen interactions; and does the cold-driven high oxygen availability in the Antarctic raise the limit on body size by reducing trade-offs between diffusivity and structural integrity? The research will explore the effects of increased ocean temperatures upon organisms that have different body sizes. In addition, it will provide training for graduate and undergraduate students affiliated with universities in EPSCOR states.", "east": 166.666, "geometry": "POINT(166.666 -77.84999)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "USAP-DC; NOT APPLICABLE", "locations": null, "north": -77.84999, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Moran, Amy; Woods, H. Arthur", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -77.84999, "title": "Collaborative Research: Body Size, Oxygen, and Vulnerability to Climate Change in Antarctic Pycnogonida", "uid": "p0000007", "west": 166.666}, {"awards": "1341612 Bowser, Samuel", "bounds_geometry": null, "dataset_titles": "Aerial survey of Explorers Cove shoreline, late January 2005; Astrammina rara genome sequencing and assembly; Astrammina triangularis genome sequencing and assembly; Crithionina delacai mitochondrial genome sequence and assembly; Scanning electron micrographs: Influence of heavy metal (Pb, Cd) exposure on shell morphogenesis in Astrammina rara, a giant agglutinated Antarctic foraminiferan protist", "datasets": [{"dataset_uid": "200090", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Astrammina rara genome sequencing and assembly", "url": "https://dataview.ncbi.nlm.nih.gov/object/PRJNA521081?reviewer=25e190ih1svottjkrrpfa7huoe"}, {"dataset_uid": "601229", "doi": "10.15784/601229", "keywords": "Aerial Imagery; Antarctica; Camera; Delta; Freshwater; Helicopter; Moat; Shoreline Survey; Small Ponds; Snow Melt; Tide Pools", "people": "Alexander, Steve; Bowser, Samuel", "repository": "USAP-DC", "science_program": null, "title": "Aerial survey of Explorers Cove shoreline, late January 2005", "url": "https://www.usap-dc.org/view/dataset/601229"}, {"dataset_uid": "601138", "doi": "10.15784/601138", "keywords": "Antarctica; Biota; Foraminifera; Heavy Metal Toxicity; Scanning Electron Microscop; Scanning Electron Microscope (SEM) Images; Scanning Electron Microscopy; Transantarctic Mountains", "people": "Andreas, Amanda; Bowser, Samuel", "repository": "USAP-DC", "science_program": null, "title": "Scanning electron micrographs: Influence of heavy metal (Pb, Cd) exposure on shell morphogenesis in Astrammina rara, a giant agglutinated Antarctic foraminiferan protist", "url": "https://www.usap-dc.org/view/dataset/601138"}, {"dataset_uid": "200091", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Crithionina delacai mitochondrial genome sequence and assembly", "url": "https://dataview.ncbi.nlm.nih.gov/object/PRJNA592714?reviewer=ivse8455h3gfaiilg4nqle0vm1"}, {"dataset_uid": "200089", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Astrammina triangularis genome sequencing and assembly", "url": "https://dataview.ncbi.nlm.nih.gov/object/PRJNA521279?reviewer=g418tpq02sif2g6do94dpmmjdv"}], "date_created": "Thu, 29 Nov 2018 00:00:00 GMT", "description": "Agglutinated foraminifera (forams for short) are early-evolving, single-celled organisms. These \"living fossils\" construct protective shells using sediment grains held together by adhesive substances that they secrete. During shell construction, agglutinated forams display amazing properties of selection - for example, some species build their shells of clear quartz grains, while other species use only grains of a specific size. Understanding how these single cells assemble complex structures may contribute to nanotechnology by enabling people to use forams as \"cellular machines\" to aid in the construction of nano-devices. This project will analyze the genomes of at least six key foram species, and then \"mine\" these genomes for technologically useful products and processes. The project will focus initially on the adhesive materials forams secrete, which may have wide application in biomedicine and biotechnology. Furthermore, the work will further develop a molecular toolkit which could open up new avenues of research on the physiology, ecology, and population dynamics of this important group of Antarctic organisms. The project will also further the NSF goals of making scientific discoveries available to the general public and of training new generations of scientists. Educational experiences related to the \"thrill of scientific exploration and discovery\" for students and the general public will be provided through freely-available short films and a traveling art/science exhibition. The project will also provide hands-on research opportunities for undergraduate students. Explorers Cove, situated on the western shore of McMurdo Sound, harbors a unique population of foraminiferan taxa at depths accessible by scuba diving that otherwise are primarily found in the deep sea. The project will use next-generation DNA sequencing and microdissection methods to obtain and analyze nuclear and mitochondrial genomes from crown members of two species each from three distinct, early-evolving foraminiferal groups. It will also use next generation sequencing methods to characterize the in-situ prokaryotic assemblages (microbiomes) of one of these groups and compare them to reference sediment microbiomes. The phyogenomic studies of the targeted Antarctic genera will help fill significant gaps in our current understanding of early foram evolution. Furthermore, comparative genomic analyses of these six species are expected to yield a better understanding of the physiology of single-chambered agglutinated forams, especially the bioadhesive proteins and regulatory factors involved in shell composition and morphogenesis. Additionally, the molecular basis of cold adaptation in forams will be examined, particularly with respect to key proteins.", "east": null, "geometry": null, "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "USAP-DC; NOT APPLICABLE", "locations": null, "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Bowser, Samuel", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "NCBI GenBank", "repositories": "NCBI GenBank; USAP-DC", "science_programs": null, "south": null, "title": "Assembling and Mining the Genomes of Giant Antarctic Foraminifera", "uid": "p0000004", "west": null}, {"awards": "1341362 Gast, Rebecca", "bounds_geometry": "POLYGON((-180 -65,-176 -65,-172 -65,-168 -65,-164 -65,-160 -65,-156 -65,-152 -65,-148 -65,-144 -65,-140 -65,-140 -66.5,-140 -68,-140 -69.5,-140 -71,-140 -72.5,-140 -74,-140 -75.5,-140 -77,-140 -78.5,-140 -80,-144 -80,-148 -80,-152 -80,-156 -80,-160 -80,-164 -80,-168 -80,-172 -80,-176 -80,180 -80,178 -80,176 -80,174 -80,172 -80,170 -80,168 -80,166 -80,164 -80,162 -80,160 -80,160 -78.5,160 -77,160 -75.5,160 -74,160 -72.5,160 -71,160 -69.5,160 -68,160 -66.5,160 -65,162 -65,164 -65,166 -65,168 -65,170 -65,172 -65,174 -65,176 -65,178 -65,-180 -65))", "dataset_titles": "Dinoflagellate sequende data", "datasets": [{"dataset_uid": "000240", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Dinoflagellate sequende data", "url": "http://www.ncbi.nlm.nih.gov/bioproject/428208"}], "date_created": "Mon, 12 Feb 2018 00:00:00 GMT", "description": "Kleptoplasty, the temporary acquisition and use of functional chloroplasts derived from algal prey, is viewed as an important model for the early evolution of the permanent, endosymbiotically-derived chloroplasts found in all permanently photosynthetic eukaryotes. This project will study the evolutionary history and expression of plastid-targeted genes in an abundant Antarctic dinoflagellate that steals chloroplasts from an ecologically important alga, the haptophyte Phaeocystis. Algae play an important role in the fixation and export of CO2 in the Southern Ocean, and this project will explore the genetic basis for the function of these chimeric cells with regard to their functional adaptation to extreme environments and will study the evolutionary history and expression of plastid-targeted genes in both the host and recipient. The project seeks to determine whether the kleptoplastidic dinoflagellate utilizes ancestral plastid proteins to regulate its stolen plastid, and how their transcription is related to environmental factors that are relevant to the Southern Ocean environment (temperature and light). To accomplish these goals, the project will utilize high throughput transcriptome analysis and RNA-sequencing experiments with the dinoflagellate and Phaeocystis. This work will help biologists understand the environmental success of this alternative nutritional strategy, and to assess the potential impact of anthropogenic climate change on the organism. The project will also contribute to the maintenance of a culture collection of heterotrophic, phototrophic and mixotrophic Antarctic protists that are available to the scientific community, and it will support the mentoring of a graduate student and a postdoctoral fellow. The work is being accomplished as an international collaboration between US and Canadian scientists, and in addition to publishing results in peer-reviewed journals, the investigators will incorporate aspects of this work into public outreach activities. These include field data analysis opportunities for middle school students and science-based art projects with local schools and museums.", "east": -140.0, "geometry": "POINT(-170 -72.5)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "NOT APPLICABLE; USAP-DC", "locations": null, "north": -65.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Gast, Rebecca", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "NCBI GenBank", "repositories": "NCBI GenBank", "science_programs": null, "south": -80.0, "title": "You are what you eat: The Role of Kleptoplasty in an Antarctic Dinoflagellate", "uid": "p0000302", "west": 160.0}, {"awards": "0732655 Mosley-Thompson, Ellen; 0732711 Smith, Craig; 0732602 Truffer, Martin; 0732983 Vernet, Maria; 0732651 Gordon, Arnold; 0732625 Leventer, Amy", "bounds_geometry": "POLYGON((-68 -57.8,-66.78 -57.8,-65.56 -57.8,-64.34 -57.8,-63.12 -57.8,-61.9 -57.8,-60.68 -57.8,-59.46 -57.8,-58.24 -57.8,-57.02 -57.8,-55.8 -57.8,-55.8 -58.8,-55.8 -59.8,-55.8 -60.8,-55.8 -61.8,-55.8 -62.8,-55.8 -63.8,-55.8 -64.8,-55.8 -65.8,-55.8 -66.8,-55.8 -67.8,-57.02 -67.8,-58.24 -67.8,-59.46 -67.8,-60.68 -67.8,-61.9 -67.8,-63.12 -67.8,-64.34 -67.8,-65.56 -67.8,-66.78 -67.8,-68 -67.8,-68 -66.8,-68 -65.8,-68 -64.8,-68 -63.8,-68 -62.8,-68 -61.8,-68 -60.8,-68 -59.8,-68 -58.8,-68 -57.8))", "dataset_titles": "Abrupt Environmental Change in the Larsen Ice Shelf System (LARISSA) - Marine Ecosystems; Biology Species Abundance from the Larsen Ice Shelf acquired during the Nathaniel B. Palmer expeditions NBP1001 and NBP1203; Bruce Plateau Accumulation O18 2009-1900; Easten Antarctic Peninsula Surface Sediment Diatom Data; LMG13-11 JKC-1 Paleoceanographic data; Macrofauna Species Abundance Raw Data from the Larsen Ice Shelf acquired during the Nathaniel B. Palmer expedition NBP1001; Megafauna Species Abundance Raw Data from the Larsen Ice Shelf acquired during the Nathaniel B. Palmer expedition NBP1001; NBP1001 cruise data; NBP1203 cruise data; Processed CTD Data from the Larsen Ice Shelf in Antarctica acquired during the Nathaniel B. Palmer expedition NBP1001; Processed CTD Data from the Larsen Ice Shelf near Antarctica acquired during the Nathaniel B. Palmer expedition NBP1203; Processed ship-based LADCP Sonar Data from the Larsen Ice Shelf in Antarctica acquired during the Nathaniel B. Palmer expedition NBP1001; Processed ship-based LADCP Sonar Data from the Larsen Ice Shelf near Antarctica acquired during the Nathaniel B. Palmer expedition NBP1203; Radioisotope data (C-14 and Pb-210) from bulk sediments, Larsen A Ice Shelf; Sediment samples (full data link not provided)", "datasets": [{"dataset_uid": "601348", "doi": null, "keywords": "Antarctica; Antarctic Peninsula; CTD; CTD Data; LARISSA; Larsen Ice Shelf; NBP1203; Oceans; Physical Oceanography; R/v Nathaniel B. Palmer; Salinity; Temperature", "people": "Gordon, Arnold; Huber, Bruce", "repository": "USAP-DC", "science_program": null, "title": "Processed CTD Data from the Larsen Ice Shelf near Antarctica acquired during the Nathaniel B. Palmer expedition NBP1203", "url": "https://www.usap-dc.org/view/dataset/601348"}, {"dataset_uid": "000226", "doi": "", "keywords": null, "people": null, "repository": "MGDS", "science_program": null, "title": "Biology Species Abundance from the Larsen Ice Shelf acquired during the Nathaniel B. Palmer expeditions NBP1001 and NBP1203", "url": "https://doi.org/10.1594/ieda/320821"}, {"dataset_uid": "601347", "doi": null, "keywords": "Antarctica; Antarctic Peninsula; Current Measurements; LADCP; LARISSA; Larsen Ice Shelf; NBP1203; Oceans; Physical Oceanography; R/v Nathaniel B. Palmer", "people": "Huber, Bruce; Gordon, Arnold", "repository": "USAP-DC", "science_program": null, "title": "Processed ship-based LADCP Sonar Data from the Larsen Ice Shelf near Antarctica acquired during the Nathaniel B. Palmer expedition NBP1203", "url": "https://www.usap-dc.org/view/dataset/601347"}, {"dataset_uid": "601485", "doi": "10.15784/601485", "keywords": "Antarctica; Antarctic Peninsula; Delta 13C; Delta 18O; Paleoceanography; Temperature", "people": "Shevenell, Amelia", "repository": "USAP-DC", "science_program": "LARISSA", "title": "LMG13-11 JKC-1 Paleoceanographic data", "url": "https://www.usap-dc.org/view/dataset/601485"}, {"dataset_uid": "000142", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "NBP1001 cruise data", "url": "https://www.rvdata.us/search/cruise/NBP1001"}, {"dataset_uid": "601306", "doi": null, "keywords": "Antarctica; Antarctic Peninsula; Biota; Box Corer; LARISSA; Larsen Ice Shelf; Macrofauna; NBP1001; Oceans; R/v Nathaniel B. Palmer; Seafloor Sampling; Species Abundance", "people": "Smith, Craig", "repository": "USAP-DC", "science_program": "LARISSA", "title": "Macrofauna Species Abundance Raw Data from the Larsen Ice Shelf acquired during the Nathaniel B. Palmer expedition NBP1001", "url": "https://www.usap-dc.org/view/dataset/601306"}, {"dataset_uid": "601336", "doi": "10.15784/601336", "keywords": "Antarctica; Carbon-14; Larsen Ice Shelf; Lead-210; Marine Sediments; Radioisotope Analysis", "people": "Taylor, Richard; DeMaster, David", "repository": "USAP-DC", "science_program": "LARISSA", "title": "Radioisotope data (C-14 and Pb-210) from bulk sediments, Larsen A Ice Shelf", "url": "https://www.usap-dc.org/view/dataset/601336"}, {"dataset_uid": "601345", "doi": null, "keywords": "Antarctica; Antarctic Peninsula; CTD; CTD Data; LARISSA; Larsen Ice Shelf; NBP1001; Oceans; Physical Oceanography; R/v Nathaniel B. Palmer; Salinity; Temperature", "people": "Huber, Bruce; Gordon, Arnold", "repository": "USAP-DC", "science_program": "LARISSA", "title": "Processed CTD Data from the Larsen Ice Shelf in Antarctica acquired during the Nathaniel B. Palmer expedition NBP1001", "url": "https://www.usap-dc.org/view/dataset/601345"}, {"dataset_uid": "601211", "doi": "10.15784/601211", "keywords": "Antarctica; Antarctic Peninsula; Benthos; Biota; Diatom; Geology/Geophysics - Other; LMG0502; Marine Geoscience; Marine Sediments; Microscope; NBP0003; NBP0107; NBP0603; NBP1203; R/v Nathaniel B. Palmer; Surface Sediment", "people": "Leventer, Amy", "repository": "USAP-DC", "science_program": "LARISSA", "title": "Easten Antarctic Peninsula Surface Sediment Diatom Data", "url": "https://www.usap-dc.org/view/dataset/601211"}, {"dataset_uid": "601305", "doi": null, "keywords": "Antarctica; Antarctic Peninsula; Biota; Box Corer; LARISSA; Larsen Ice Shelf; Macrofauna; Megafauna; NBP1001; Oceans; R/v Nathaniel B. Palmer; Seafloor Sampling; Species Abundance", "people": "Smith, Craig", "repository": "USAP-DC", "science_program": "LARISSA", "title": "Megafauna Species Abundance Raw Data from the Larsen Ice Shelf acquired during the Nathaniel B. Palmer expedition NBP1001", "url": "https://www.usap-dc.org/view/dataset/601305"}, {"dataset_uid": "000143", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "NBP1203 cruise data", "url": "https://www.rvdata.us/search/cruise/NBP1203"}, {"dataset_uid": "601346", "doi": null, "keywords": "Antarctica; Antarctic Peninsula; Current Measurements; LADCP; Larsen Ice Shelf; NBP1001; Oceans; Physical Oceanography; R/v Nathaniel B. Palmer", "people": "Huber, Bruce; Gordon, Arnold", "repository": "USAP-DC", "science_program": "LARISSA", "title": "Processed ship-based LADCP Sonar Data from the Larsen Ice Shelf in Antarctica acquired during the Nathaniel B. Palmer expedition NBP1001", "url": "https://www.usap-dc.org/view/dataset/601346"}, {"dataset_uid": "600073", "doi": "10.15784/600073", "keywords": "Antarctica; Antarctic Peninsula; Araon1304; Biota; LARISSA; Larsen B Ice Shelf; NBP1001; NBP1203; Oceans; Physical Oceanography; Southern Ocean; Weddell Sea", "people": "Vernet, Maria", "repository": "USAP-DC", "science_program": "LARISSA", "title": "Abrupt Environmental Change in the Larsen Ice Shelf System (LARISSA) - Marine Ecosystems", "url": "https://www.usap-dc.org/view/dataset/600073"}, {"dataset_uid": "000145", "doi": "", "keywords": null, "people": null, "repository": "AMGRF", "science_program": null, "title": "Sediment samples (full data link not provided)", "url": "http://arf.fsu.edu/"}, {"dataset_uid": "600167", "doi": "10.15784/600167", "keywords": "Antarctica; Antarctic Peninsula; Bruce Plateau; Glaciology; Ice Core Records; Isotope; LARISSA; Paleoclimate; Sample/collection Description; Sample/Collection Description; Snow Accumulation", "people": "Mosley-Thompson, Ellen; Thompson, Lonnie G.", "repository": "USAP-DC", "science_program": "LARISSA", "title": "Bruce Plateau Accumulation O18 2009-1900", "url": "https://www.usap-dc.org/view/dataset/600167"}], "date_created": "Thu, 01 Feb 2018 00:00:00 GMT", "description": "Like no other region on Earth, the northern Antarctic Peninsula represents a spectacular natural laboratory of climate change and provides the opportunity to study the record of past climate and ecological shifts alongside the present-day changes in one of the most rapidly warming regions on Earth. This award supports the cryospheric and oceano-graphic components of an integrated multi-disciplinary program to address these rapid and fundamental changes now taking place in Antarctic Peninsula (AP). By making use of a marine research platform (the RV NB Palmer and on-board helicopters) and additional logistical support from the Argentine Antarctic program, the project will bring glaciologists, oceanographers, marine geologists and biologists together, working collaboratively to address fundamentally interdisciplinary questions regarding climate change. The project will include gathering a new, high-resolution paleoclimate record from the Bruce Plateau of Graham Land, and using it to compare Holocene- and possibly glacial-epoch climate to the modern period; investigating the stability of the remaining Larsen Ice Shelf and rapid post-breakup glacier response ? in particular, the roles of surface melt and ice-ocean interactions in the speed-up and retreat; observing the contribution of, and response of, oceanographic systems to ice shelf disintegration and ice-glacier interactions. Helicopter support on board will allow access to a wide range of glacial and geological areas of interest adjacent to the Larsen embayment. At these locations, long-term in situ glacial monitoring, isostatic uplift, and ice flow GPS sites will be established, and high-resolution ice core records will be obtained using previously tested lightweight drilling equipment. Long-term monitoring of deep water outflow will, for the first time, be integrated into changes in ice shelf extent and thickness, bottom water formation, and multi-level circulation by linking near-source observations to distal sites of concentrated outflow. The broader impacts of this international, multidisciplinary effort are that it will significantly advance our understanding of linkages amongst the earth\u0027s systems in the Polar Regions, and are proposed with international participation (UK, Spain, Belgium, Germany and Argentina) and interdisciplinary engagement in the true spirit of the International Polar Year (IPY). It will also provide a means of engaging and educating the public in virtually all aspects of polar science and the effects of ongoing climate change. The research team has a long record of involving undergraduates in research, educating high-performing graduate students, and providing innovative and engaging outreach products to the K-12 education and public media forums. Moreover, forging the new links both in science and international Antarctic programs will provide a continuing legacy, beyond IPY, of improved understanding and cooperation in Antarctica.", "east": -55.8, "geometry": "POINT(-61.9 -62.8)", "instruments": "EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE; IN SITU/LABORATORY INSTRUMENTS \u003e CORERS \u003e BOX CORE; IN SITU/LABORATORY INSTRUMENTS \u003e CORERS \u003e CORING DEVICES; IN SITU/LABORATORY INSTRUMENTS \u003e CORERS \u003e ICE AUGERS; IN SITU/LABORATORY INSTRUMENTS \u003e CORERS \u003e SNOW DENSITY CUTTER", "is_usap_dc": true, "keywords": "Paleoclimate; Megafauna; USAP-DC; AMD; Amd/Us; Antarctica; Climate Change; LABORATORY; Climate Variability; Multi-Disciplinary; Cryosphere; NBP1001; FIELD SURVEYS; Not provided; Antarctic Peninsula; R/V NBP; FIELD INVESTIGATION; USA/NSF; Ice Core; Holocene", "locations": "Antarctica; Antarctic Peninsula", "north": -57.8, "nsf_funding_programs": "Antarctic Integrated System Science; Antarctic Organisms and Ecosystems; Antarctic Integrated System Science; Antarctic Integrated System Science; Antarctic Integrated System Science; Antarctic Organisms and Ecosystems; Antarctic Integrated System Science; Antarctic Integrated System Science", "paleo_time": "PHANEROZOIC \u003e CENOZOIC \u003e QUATERNARY \u003e HOLOCENE", "persons": "Truffer, Martin; Gordon, Arnold; Huber, Bruce; Mosley-Thompson, Ellen; Leventer, Amy; Vernet, Maria; Smith, Craig; Thompson, Lonnie G.", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION; LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS; Not provided; OTHER \u003e PHYSICAL MODELS \u003e LABORATORY; WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repo": "USAP-DC", "repositories": "AMGRF; MGDS; R2R; USAP-DC", "science_programs": "LARISSA", "south": -67.8, "title": "Collaborative Research in IPY: Abrupt Environmental Change in the Larsen Ice Shelf System, a Multidisciplinary Approach -- Cryosphere and Oceans", "uid": "p0000101", "west": -68.0}, {"awards": "1245703 Manahan, Donal", "bounds_geometry": "POLYGON((-68.0574 -52.7267,-67.39775 -52.7267,-66.7381 -52.7267,-66.07845 -52.7267,-65.4188 -52.7267,-64.75915 -52.7267,-64.0995 -52.7267,-63.43985 -52.7267,-62.7802 -52.7267,-62.12055 -52.7267,-61.4609 -52.7267,-61.4609 -53.95849,-61.4609 -55.19028,-61.4609 -56.42207,-61.4609 -57.65386,-61.4609 -58.88565,-61.4609 -60.11744,-61.4609 -61.34923,-61.4609 -62.58102,-61.4609 -63.81281,-61.4609 -65.0446,-62.12055 -65.0446,-62.7802 -65.0446,-63.43985 -65.0446,-64.0995 -65.0446,-64.75915 -65.0446,-65.4188 -65.0446,-66.07845 -65.0446,-66.7381 -65.0446,-67.39775 -65.0446,-68.0574 -65.0446,-68.0574 -63.81281,-68.0574 -62.58102,-68.0574 -61.34923,-68.0574 -60.11744,-68.0574 -58.88565,-68.0574 -57.65386,-68.0574 -56.42207,-68.0574 -55.19028,-68.0574 -53.95849,-68.0574 -52.7267))", "dataset_titles": "Expedition Data", "datasets": [{"dataset_uid": "001372", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG1606"}], "date_created": "Fri, 29 Dec 2017 00:00:00 GMT", "description": "This project will support two training courses that will introduce early-career scientists from a wide range of disciplinary backgrounds to key issues in polar science, and especially to provide the opportunity to gain hands-on experience in Antarctic field activities. Antarctica is an ideal location to study a wide variety of questions in biology. However, few students and early-career scientists have the opportunity to work on-site in Antarctica unless they are directly associated with a senior scientist who has a funded Antarctic project. The project will further the NSF goal of training new generations of scientists by providing hands-on training in Antarctica during one course at Palmer Station in 2016 and another at McMurdo Station in 2018. This represents a continuation of nine previous courses at McMurdo Station which have a proven record of introducing participants to Antarctic science under realistic field conditions, providing opportunities to understand and appreciate the complexities and logistical challenges of undertaking science in Antarctica, enhancing the professional careers of the participants, and increasing international collaborations for early-career scientists. The proposed training courses will be open to Ph.D. students and post-doctoral scientists who have interests in the study of Antarctic marine organisms to help prepare them for success in developing their own independent research programs in polar regions. Long-standing and recent questions in evolution and ecology of Antarctic organisms will be examined with 1) field collections, 2) physiological experiments on whole organisms, 3) studies of isolated cells and tissues, 4) experiments on macromolecular processes (e.g., enzymes), and 5) molecular biological analyses.", "east": -61.4609, "geometry": "POINT(-64.75915 -58.88565)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; IN SITU/LABORATORY INSTRUMENTS \u003e CHEMICAL METERS/ANALYZERS \u003e FLUOROMETERS; IN SITU/LABORATORY INSTRUMENTS \u003e RECORDERS/LOGGERS \u003e AWS; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e XBT; EARTH REMOTE SENSING INSTRUMENTS \u003e ACTIVE REMOTE SENSING \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ECHO SOUNDERS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e THERMOSALINOGRAPHS", "is_usap_dc": false, "keywords": "R/V LMG; LMG1606", "locations": null, "north": -52.7267, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Manahan, Donal", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "R2R", "repositories": "R2R", "science_programs": null, "south": -65.0446, "title": "Collaborative Research: Biological Adaptations to Environmental Change in Antarctica - An Advanced Training Program for Early Career Scientists", "uid": "p0000392", "west": -68.0574}, {"awards": "1341701 Bilyk, Kevin", "bounds_geometry": null, "dataset_titles": "Antarctic Ice fish; submission ID #SRP113562", "datasets": [{"dataset_uid": "000206", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Antarctic Ice fish; submission ID #SRP113562", "url": "https://www.ncbi.nlm.nih.gov/genbank/"}], "date_created": "Mon, 31 Jul 2017 00:00:00 GMT", "description": "This work will broaden our knowledge and insights into genetic trait loss or change accompanying species evolution in general as well as within the uniquely isolated and frigid Southern Ocean. The system of oxygen-carrying and related proteins being studied is very important to human health and the two proteins being specifically studied in this work (haptoglobin and hemopexin) have crucial roles in preventing excess iron loading in the kidneys. As such, the project has the potential to contribute novel insights that could be valuable to medical science. The project will also further the NSF goals of training new generations of scientists and of making scientific discoveries available to the general public. The lead principal investigator on the project is an early career scientist whose career development will be enhanced by this project. It will also support the training of several undergraduate students in molecular biology, protein biochemistry, and appreciation of the unique Antarctic fish fauna and environment. The project will contribute to a content-rich web site that will bring to the public the history of biological discoveries and sciences on fishes of the Southern Ocean and through this project the investigators will contribute to an annual polar event at a children\u0027s science museum. The Antarctic icefishes have thrived despite the striking evolutionary loss of the normally indispensable respiratory protein hemoglobin in all species and myoglobin in some. Studies over the past decades have predominately focused on the mechanisms behind hemoprotein losses and the resulting compensatory adaptations in these fish, while evolutionary impact of such losses on the supporting protein genes and functions has remained unaddressed. This project investigates the evolutionary fate of two important partner proteins, the hemoglobin scavenger haptoglobin and the heme scavenger hemopexin (heme groups are the iron-containing functional group of proteins such as hemoglobin and myoglobin). With the permanent hemoglobin-null state in Antarctic icefishes, and particularly in dual hemoglobin- and myoglobin-null species, the preservation of a functional haptoglobin would seem unessential and the role of hemopexin likely diminished. This project seeks to resolve whether co-evolutionary loss or reduction of these supporting proteins occurred with the extinction of the hemoglobin trait in the icefishes, and the molecular mechanisms underlying such changes. The investigators envisage the cold and oxygen rich marine environment as the start of a cascade of relaxation of selection pressures. Initially this would have obviated the need for maintaining functional oxygen carrying proteins, ultimately leading to their permanent loss. These events in turn would have relaxed the maintenance of the network of supporting systems, leading to additional trait loss or change.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Bilyk, Kevin", "platforms": "Not provided", "repo": "NCBI GenBank", "repositories": "NCBI GenBank", "science_programs": null, "south": null, "title": "Evolutionary Fates of Hemoglobin and Heme Scavengers in White-blooded Antarctic Icefishes", "uid": "p0000396", "west": null}, {"awards": "1443554 Buys, Emmanuel", "bounds_geometry": "POLYGON((166.163 -76.665,166.2635 -76.665,166.364 -76.665,166.4645 -76.665,166.565 -76.665,166.6655 -76.665,166.766 -76.665,166.8665 -76.665,166.967 -76.665,167.0675 -76.665,167.168 -76.665,167.168 -76.782,167.168 -76.899,167.168 -77.016,167.168 -77.133,167.168 -77.25,167.168 -77.367,167.168 -77.484,167.168 -77.601,167.168 -77.718,167.168 -77.835,167.0675 -77.835,166.967 -77.835,166.8665 -77.835,166.766 -77.835,166.6655 -77.835,166.565 -77.835,166.4645 -77.835,166.364 -77.835,166.2635 -77.835,166.163 -77.835,166.163 -77.718,166.163 -77.601,166.163 -77.484,166.163 -77.367,166.163 -77.25,166.163 -77.133,166.163 -77.016,166.163 -76.899,166.163 -76.782,166.163 -76.665))", "dataset_titles": "Biosamples and observations from Weddell Seal colonies in McMurdo Sound during the 2015-2016 Antarctic field season", "datasets": [{"dataset_uid": "601028", "doi": "10.15784/601028", "keywords": "Antarctica; Biota; McMurdo Sound; Ross Sea; Sample/collection Description; Sample/Collection Description; Seals", "people": "Buys, Emmanuel; Hindle, Allyson", "repository": "USAP-DC", "science_program": null, "title": "Biosamples and observations from Weddell Seal colonies in McMurdo Sound during the 2015-2016 Antarctic field season", "url": "https://www.usap-dc.org/view/dataset/601028"}], "date_created": "Fri, 26 May 2017 00:00:00 GMT", "description": "The Weddell seal is a champion diving mammal. The physiology that permits these animals to sustain extended breath-hold periods and survive the extreme pressure of diving deep allows them to thrive in icy Antarctic waters. Key elements of their physiological specializations to breath-hold diving are their ability for remarkable adjustment of their heart and blood vessel system, coordinating blood pressure and flow to specific body regions based on their metabolic requirements, and their ability to sustain periods without oxygen. Identifying the details of these strategies has tremendous potential to better inform human medicine, helping us to develop novel therapies for cardiovascular trauma (e.g. stroke, heart attack) and diseases associated with blunted oxygen delivery to tissues (e.g. pneumonia, sepsis, or cancer). The goal of this project is to document specific genes that control these cardiovascular adjustments in seals, and to compare their abundance and activity with humans. Specifically, the investigators will study a signaling pathway that coordinates local blood flow. They will also use tissue samples to generate cultured cells from Weddell seals that can be used to study the molecular effects of low oxygen conditions in the laboratory. The project will further the NSF goals of training new generations of scientists and of making scientific discoveries available to the general public. The project will train a pre-veterinary student researcher will conduct public outreach via a center for community health improvement, a multicultural affairs office, and a public aquarium. The goal of this study is to unravel the molecular mechanisms underlying the dive response. A hallmark of the dive response is tissue-specific vascular system regulation, likely resulting from variation in both nerve inputs and in production of local signaling molecules produced by blood vessel cells. The investigators will use emerging genomic information to begin to unravel the genetics underlying redistribution of the circulation during diving. They will also directly test the hypothesis that modifications in the signaling system prevent local blood vessel changes under low oxygen conditions, thereby allowing the centrally mediated diving reflex to override local physiological responses and to control the constriction of blood vessel walls in Weddell seals. They will perform RNA-sequencing of Weddell seal tissues and use the resulting sequence, along with information from other mammals such as dog, to obtain a full annotation (identifying all genes based on named features of reference genomes) of the existing genome assembly for the Weddell seal, facilitating comparative and species-specific genomic research. They will also generate a Weddell seal pluripotent stem cell line which should be a valuable research tool for cell biologists, molecular biologists and physiologists that will allow them to further test their hypotheses. It is expected that the proposed studies will advance our knowledge of the biochemical and physiological adaptations that allow the Weddell seal to thrive in the Antarctic environment.", "east": 167.168, "geometry": "POINT(166.6655 -77.25)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -76.665, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Buys, Emmanuel; Costa, Daniel; Zapol, Warren; Hindle, Allyson", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -77.835, "title": "Unraveling the Genomic and Molecular Basis of the Dive Response: Nitric Oxide Signaling and Vasoregulation in the Weddell Seal", "uid": "p0000072", "west": 166.163}, {"awards": "1142018 Arrigo, Kevin", "bounds_geometry": "POLYGON((-75.8 -61.08,-74.457 -61.08,-73.114 -61.08,-71.771 -61.08,-70.428 -61.08,-69.085 -61.08,-67.742 -61.08,-66.399 -61.08,-65.056 -61.08,-63.713 -61.08,-62.37 -61.08,-62.37 -61.684,-62.37 -62.288,-62.37 -62.892,-62.37 -63.496,-62.37 -64.1,-62.37 -64.704,-62.37 -65.308,-62.37 -65.912,-62.37 -66.516,-62.37 -67.12,-63.713 -67.12,-65.056 -67.12,-66.399 -67.12,-67.742 -67.12,-69.085 -67.12,-70.428 -67.12,-71.771 -67.12,-73.114 -67.12,-74.457 -67.12,-75.8 -67.12,-75.8 -66.516,-75.8 -65.912,-75.8 -65.308,-75.8 -64.704,-75.8 -64.1,-75.8 -63.496,-75.8 -62.892,-75.8 -62.288,-75.8 -61.684,-75.8 -61.08))", "dataset_titles": "Adaptive Responses of Phaeocystis Populations in Antarctic Ecosystems; Expedition Data", "datasets": [{"dataset_uid": "001417", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/NBP1310"}, {"dataset_uid": "600161", "doi": "10.15784/600161", "keywords": "Antarctica; Antarctic Peninsula; Biota; Chlorophyll; CTD Data; NBP1310; NBP1409; Oceans; Physical Oceanography; Phytoplankton; Sea Surface; Southern Ocean", "people": "Arrigo, Kevin", "repository": "USAP-DC", "science_program": null, "title": "Adaptive Responses of Phaeocystis Populations in Antarctic Ecosystems", "url": "https://www.usap-dc.org/view/dataset/600161"}], "date_created": "Mon, 11 Jan 2016 00:00:00 GMT", "description": "Global climate change is having significant effects on areas of the Southern Ocean, and a better understanding of this ecosystem will permit predictions about the large-scale implications of these shifts. The haptophyte Phaeocystis antarctica is an important component of the phytoplankton communities in this region, but little is known about the factors controlling its distribution. Preliminary data suggest that P. antarctica posses unique adaptations that allow it to thrive in regions with dynamic light regimes. This research will extend these results to identify the physiological and genetic mechanisms that affect the growth and distribution of P. antarctica. This work will use field and laboratory-based studies and a suite of modern molecular techniques to better understand the biogeography and physiology of this key organism. Results will be widely disseminated through publications as well as through presentations at national and international meetings. In addition, raw data will be made available through open-access databases. This project will support the research and training of two graduate students and will foster an established international collaboration with Dutch scientists. Researchers on this project will participate in outreach programs targeting K12 teachers as well as high school students.", "east": -62.37, "geometry": "POINT(-69.085 -64.1)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; IN SITU/LABORATORY INSTRUMENTS \u003e CHEMICAL METERS/ANALYZERS \u003e FLUOROMETERS; IN SITU/LABORATORY INSTRUMENTS \u003e RECORDERS/LOGGERS \u003e AWS; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; EARTH REMOTE SENSING INSTRUMENTS \u003e ACTIVE REMOTE SENSING \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ECHO SOUNDERS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e MBES; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e THERMOSALINOGRAPHS", "is_usap_dc": true, "keywords": "R/V NBP; Not provided", "locations": null, "north": -61.08, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Arrigo, Kevin", "platforms": "Not provided; WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repo": "R2R", "repositories": "R2R; USAP-DC", "science_programs": null, "south": -67.12, "title": "Collaborative Research: Adaptive Responses of Phaeocystis Populations in Antarctic Ecosystems", "uid": "p0000446", "west": -75.8}, {"awards": "0944557 Marsh, Adam", "bounds_geometry": "POINT(166 78)", "dataset_titles": "Environmental Genomics of an Antarctic Polychaete #SRP040946", "datasets": [{"dataset_uid": "000223", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Environmental Genomics of an Antarctic Polychaete #SRP040946", "url": "https://www.ncbi.nlm.nih.gov/sra/?term=SRP040946"}], "date_created": "Tue, 13 Jan 2015 00:00:00 GMT", "description": "Genome-enabled biology provides a foundation for understanding the genetic basis of organism-environment interactions. . The research project links gene expression, genome methylation, and metabolic rates to assess the mechanisms of environmental adaptation (temperature) across multiple generations in a polar, and closely related temperate, polychaete. By comparing these two species, the research will assess how a polar environment shapes responses to environmental stress. This work will produce: 1) a database of full transcriptome (gene specific) profiling data for the polar polychaete cultured at two temperatures; 2) the contribution of genome methylation to the suppression of gene transcription activities; 3) the linkage between shifts in mRNA pools and total cellular activities (as ATP consumption via respiration); 4) an assessment of the inheritance of patterns of gene expression and metabolic activities across three generations; and 5) a simple demographic model of the polar polychaete population dynamics under normal and \u0027global-warming\u0027 temperature scenarios. Broader impacts include two outreach activities. The first is a mentoring program, where African-American undergraduate students spend 1.5 years working on a research project with a UD faculty member (2 summers plus their senior academic year). The second is a children\u0027s display activity at UD?s School of Marine Science \"Coast Day\".", "east": 166.0, "geometry": "POINT(166 -78)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -78.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Marsh, Adam G.", "platforms": "Not provided", "repo": "NCBI GenBank", "repositories": "NCBI GenBank", "science_programs": null, "south": -78.0, "title": "Environmental Genomics in an Antarctic polychaete", "uid": "p0000355", "west": 166.0}, {"awards": "1447291 Place, Sean; 1040957 Sarmiento, Jorge; 1040945 Place, Sean", "bounds_geometry": "POLYGON((-180 90,-144 90,-108 90,-72 90,-36 90,0 90,36 90,72 90,108 90,144 90,180 90,180 72,180 54,180 36,180 18,180 0,180 -18,180 -36,180 -54,180 -72,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -72,-180 -54,-180 -36,-180 -18,-180 0,-180 18,-180 36,-180 54,-180 72,-180 90))", "dataset_titles": "Does the strength of the carbonate pump change with ocean stratification and acidification and how? Project data; NCBI GenBank RNA sequences, Pagothenia borchgrevinki; NCBI GenBank RNA sequences, Trematomus bernacchii; NCBI GenBank RNA sequences, Trematomus newnesi; NCBI links to BioProjects of total RNA isolated from Trematomus bernacchii gill tissues acclimated to elevated temperature and pCO2, July 2015", "datasets": [{"dataset_uid": "000166", "doi": "", "keywords": null, "people": null, "repository": "BCO-DMO", "science_program": null, "title": "NCBI links to BioProjects of total RNA isolated from Trematomus bernacchii gill tissues acclimated to elevated temperature and pCO2, July 2015", "url": "http://www.bco-dmo.org/dataset/665853"}, {"dataset_uid": "000165", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "NCBI GenBank RNA sequences, Trematomus newnesi", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA294787"}, {"dataset_uid": "000186", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "NCBI GenBank RNA sequences, Trematomus newnesi", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA294787"}, {"dataset_uid": "000164", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "NCBI GenBank RNA sequences, Pagothenia borchgrevinki", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA294774"}, {"dataset_uid": "000219", "doi": "", "keywords": null, "people": null, "repository": "BCO-DMO", "science_program": null, "title": "Does the strength of the carbonate pump change with ocean stratification and acidification and how? Project data", "url": "http://www.bco-dmo.org/project/521216"}, {"dataset_uid": "000163", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "NCBI GenBank RNA sequences, Trematomus bernacchii", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA289753"}, {"dataset_uid": "000184", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "NCBI GenBank RNA sequences, Trematomus bernacchii", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA289753"}, {"dataset_uid": "000185", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "NCBI GenBank RNA sequences, Pagothenia borchgrevinki", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA294774"}], "date_created": "Mon, 12 Jan 2015 00:00:00 GMT", "description": "The proposed research will investigate the interacting and potentially synergistic influence of two oceanographic features - ocean acidification and the projected rise in mean sea surface temperature - on the performance of Notothenioids, the dominant fish of the Antarctic marine ecosystem. Understanding the joint effects of acidification and temperature rise on these fish is a vital component of predicting the resilience of coastal marine ecosystems. Notothenioids have repeatedly displayed a narrow window of physiological tolerances when subjected to abiotic stresses. Given that evolutionary adaptation may have led to finely-tuned traits with narrow physiological limits in these organisms, this system provides a unique opportunity to examine physiological trade-offs associated with acclimation to the multi-stressor environment expected from future atmospheric CO2 projections. Understanding these trade-offs will provide valuable insight into the capacity species have for responses to climate change via phenotypic plasticity. As an extension to functional measurements, this study will use evolutionary approaches to map variation in physiological responses onto the phylogeny of these fishes and the genetic diversity within species. These approaches offer insight into the historical constraints and future potential for evolutionary optimization. The research will significantly expand the genomic resources available to polar researchers and will support the training of graduate students and a post doc at an EPSCoR institution. Research outcomes will be incorporated into classroom curriculum.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": 90.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems; Antarctic Integrated System Science; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Place, Sean; Sarmiento, Jorge; Dudycha, Jeffry; Kwon, Eun-Young", "platforms": "Not provided", "repo": "BCO-DMO", "repositories": "BCO-DMO; NCBI GenBank", "science_programs": null, "south": -90.0, "title": "Ocean Acidification Category 1: Identifying Adaptive Responses of Polar Fishes in a Vulnerable Ecosystem", "uid": "p0000006", "west": -180.0}, {"awards": "0837559 Lee, Richard", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "Belgica antarctica isolate:Palmer_Station_2011 Genome sequencing and assembly", "datasets": [{"dataset_uid": "000147", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Belgica antarctica isolate:Palmer_Station_2011 Genome sequencing and assembly", "url": "http://www.ncbi.nlm.nih.gov/bioproject/PRJNA172148"}], "date_created": "Thu, 16 Oct 2014 00:00:00 GMT", "description": "This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).\u003cbr/\u003e\u003cbr/\u003ePolar terrestrial environments are often described as deserts, where water availability is a critical factor limiting the distribution of terrestrial organisms. In such environments, tolerance of low moisture conditions is likely as important as cold resistance. Winter survival for many polar organisms depends on a coordinated transition from feeding, growth and reproduction during short summers, to an energy-conserving dormancy coupled with enhanced resistance to environmental extremes during long, severe winters. The midge Belgica antarctica provides an excellent model system for investigating mechanisms of stress (cold and low moisture) tolerance, and the role of extreme photoperiodic changes in coordinating seasonal adaptations. The proposed research will use gene and protein level approaches to investigate the seasonal roles of dehydration and photoperiodic cues in preparing a polar insect for winter survival. The research will investigate (1) the role of aquaporins, dehydrins, and cryoprotective dehydration in seasonal survival, and (2) the role of photoperiodism in preparing for winter. Broader impacts involve engagement of K-12 educators and students, including hands-on, in-the-field research experiences for teachers, presentations at local schools, development of lesson plans and podcasts, and publication of articles in education journals. The principal investigators also will engage graduate students, undergraduates, and post-docs in the project.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Lee, Richard", "platforms": "Not provided", "repo": "NCBI GenBank", "repositories": "NCBI GenBank", "science_programs": null, "south": -90.0, "title": "Collaborative Proposal: Roles for Dehydration and Photoperiodism in Preparing an Antarctic Insect for the Polar Night", "uid": "p0000669", "west": -180.0}, {"awards": "0943935 Isbell, John; 0943934 Taylor, Edith", "bounds_geometry": null, "dataset_titles": "Portal to search geologic sample collections, Polar Rock Repository, Byrd Polar Research Center, The Ohio State University; Portal to search paleobotanical collections, Biodiversity Institute, University of Kansas", "datasets": [{"dataset_uid": "001402", "doi": "", "keywords": null, "people": null, "repository": "PI website", "science_program": null, "title": "Portal to search paleobotanical collections, Biodiversity Institute, University of Kansas", "url": "http://biodiversity.ku.edu/paleobotany/collections/collections-search"}, {"dataset_uid": "001377", "doi": "", "keywords": null, "people": null, "repository": "PRR", "science_program": null, "title": "Portal to search geologic sample collections, Polar Rock Repository, Byrd Polar Research Center, The Ohio State University", "url": "http://research.bpcrc.osu.edu/rr/"}, {"dataset_uid": "002567", "doi": "", "keywords": null, "people": null, "repository": "PI website", "science_program": null, "title": "Portal to search paleobotanical collections, Biodiversity Institute, University of Kansas", "url": "http://biodiversity.ku.edu/paleobotany/collections/collections-search"}], "date_created": "Tue, 23 Sep 2014 00:00:00 GMT", "description": "Intellectual Merit:\u003cbr/\u003eThe focus of this proposal is to collect fossil plants and palynomorphs from Permian-Triassic (P-T) rocks of the central Transantarctic Mountains (CTM), together with detailed data on sedimentologic and paleoecologic depositional environments. Fossil plants are important climate proxies that offer a unique window into the past, and the CTM fossils are an important source of data on the ways that plants responded to a strongly seasonal, polar light regime during a time of global change. The proposed project uses paleobotanical expertise, integrated with detailed sedimentology and stratigraphy, to reconstruct Permian-Triassic plant communities and their paleoenvironments. This interdisciplinary approach could uncover details of Antarctica?s complex late Paleozoic and Mesozoic environmental and climatic history which included: 1) deglaciation, 2) development and evolution of a post-glacial landscape and biota, 3) environmental and biotic change associated with the end-Permian mass extinction, 4) environmental recovery in the earliest Triassic, 5) strong, possible runaway Triassic greenhouse, and 6) widespread orogenesis and development of a foreland basin system. The PIs will collect compression floras both quantitatively and qualitatively to obtain biodiversity and abundance data. Since silicified wood is also present, the PIs will analyze tree rings and growth in a warm, high-latitude environment for which there is no modern analogue. Fossil plants from the CTM can provide biological and environmental information to: 1) interpret paleoclimate when Gondwana moved from icehouse to greenhouse conditions; 2) trace floral evolution across the P-T boundary; 3) reconstruct Antarctic plant life; 4) further understanding of plant adaptations to high latitudes. The Intellectual Merit of the research includes: 1) tracing floral evolution after the retreat of glaciers; 2) examining floral composition and diversity across the PTB; and 3) obtaining data on the recovery of these ecosystems in the Early Triassic, as well as changes in floral cover and diversity in the Early-Middle Triassic. Antarctica is the only place on Earth that includes extensive outcrops of terrestrial rocks, combined with widespread and well-preserved plant fossils, which spans this crucial time period.\u003cbr/\u003e\u003cbr/\u003eBroader impacts:\u003cbr/\u003eThe broader impacts include public outreach; teaching, and mentoring of women and underrepresented students; mentoring graduate student, postdoctoral, and new faculty women; development of an inquiry-based workshop on Antarctic paleoclimate with the Division of Education, KU Natural History Museum; continuing support of workshops for middle school girls in science via the Expanding Your Horizons Program, Emporia State University, and the TRIO program, KU; exploring Antarctic geosciences through video/computer links from McMurdo Station and satellite phone conferences from the field with K-12 science classes in Wisconsin and Kansas, and through participation in the NSF Research Experiences for Teachers program at the University of Wisconsin.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": false, "keywords": "FIELD SURVEYS; LABORATORY; Transanatarctic Basin; Paleobotany; Fossil Plants; FIELD INVESTIGATION; Sedimentology; Late Paleozoic Ice Age; Not provided; Central Transantarctic Mountains; Beardmore Glacier", "locations": "Transanatarctic Basin; Central Transantarctic Mountains; Beardmore Glacier", "north": null, "nsf_funding_programs": "Antarctic Earth Sciences; Antarctic Instrumentation and Support; Antarctic Earth Sciences", "paleo_time": "PHANEROZOIC \u003e PALEOZOIC \u003e PERMIAN; PHANEROZOIC \u003e MESOZOIC \u003e TRIASSIC; PHANEROZOIC \u003e PALEOZOIC \u003e PERMIAN; PHANEROZOIC \u003e MESOZOIC \u003e TRIASSIC", "persons": "Isbell, John", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION; LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS; Not provided; OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repo": "PI website", "repositories": "PI website; PRR", "science_programs": null, "south": null, "title": "Collaborative Research: Antarctic Ecosystems across the Permian-Triassic Boundary: Integrating Paleobotany, Sedimentology, and Paleoecology", "uid": "p0000372", "west": null}, {"awards": "1043532 Grzymski, Joseph", "bounds_geometry": "POINT(-64 -64.7)", "dataset_titles": "NCBI GenBank Sequences# PRJNA244317, PRJNA242746", "datasets": [{"dataset_uid": "000168", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "NCBI GenBank Sequences# PRJNA244317, PRJNA242746", "url": "http://www.ncbi.nlm.nih.gov/genbank/"}], "date_created": "Mon, 08 Sep 2014 00:00:00 GMT", "description": "The proposed research will investigate the genomic basis of the physiological and ecological transition of Antarctic marine phytoplankton from a cold dark winter to a warmer, brighter spring. During a field season at Palmer Station, functional genomics (using next generation sequencing technology to identify expressed genes) and in situ fluorometry (FRRF) will be integrated with classical ecological methods to investigate photosynthetic adaptation during phytoplankton species succession from late winter into spring. Using large data sets, this project will test whether amino acid usages differ based on expression. The specific objectives are (1) To characterize phytoplankton succession from the winter to spring transition, and (2) To correlate community gene expression profiles to adaptational differences among taxa. Broader impacts include training of a post doctoral researcher and two undergraduate science majors, with efforts to attract students from underrepresented groups. The P.I.s also will prepare presentations for the public, regarding research experiences, research results, and the importance of climate change.", "east": -64.0, "geometry": "POINT(-64 -64.7)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -64.7, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Grzymski, Joseph", "platforms": "Not provided", "repo": "NCBI GenBank", "repositories": "NCBI GenBank", "science_programs": null, "south": -64.7, "title": "Collaborative Research: Functional Genomics and Physiological Ecology of Seasonal Succession in Antarctic Phytoplankton: Adaptations to Light and Temperature", "uid": "p0000462", "west": -64.0}, {"awards": "0944517 Detrich, H. William", "bounds_geometry": "POLYGON((-67.42 -61.2,-66.28 -61.2,-65.14 -61.2,-64 -61.2,-62.86 -61.2,-61.72 -61.2,-60.58 -61.2,-59.44 -61.2,-58.3 -61.2,-57.16 -61.2,-56.02 -61.2,-56.02 -61.71,-56.02 -62.22,-56.02 -62.73,-56.02 -63.24,-56.02 -63.75,-56.02 -64.26,-56.02 -64.77,-56.02 -65.28,-56.02 -65.79,-56.02 -66.3,-57.16 -66.3,-58.3 -66.3,-59.44 -66.3,-60.58 -66.3,-61.72 -66.3,-62.86 -66.3,-64 -66.3,-65.14 -66.3,-66.28 -66.3,-67.42 -66.3,-67.42 -65.79,-67.42 -65.28,-67.42 -64.77,-67.42 -64.26,-67.42 -63.75,-67.42 -63.24,-67.42 -62.73,-67.42 -62.22,-67.42 -61.71,-67.42 -61.2))", "dataset_titles": "Cruise LMG1203; Cruise LMG1205; Cruise LMG1206; GenBank KC594078, FJ914563.1, FJ914564.1, FJ914565.1, FJ896018.1, FJ896019.1, FJ896022.1, FJ896023.1, FJ896024.1, FJ896025.1, FJ896026.1", "datasets": [{"dataset_uid": "000162", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Cruise LMG1206", "url": "https://www.rvdata.us/search/cruise/LMG1206"}, {"dataset_uid": "000159", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "GenBank KC594078, FJ914563.1, FJ914564.1, FJ914565.1, FJ896018.1, FJ896019.1, FJ896022.1, FJ896023.1, FJ896024.1, FJ896025.1, FJ896026.1", "url": "http://www.ncbi.nlm.nih.gov/genbank/"}, {"dataset_uid": "000160", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Cruise LMG1203", "url": "https://www.rvdata.us/search/cruise/LMG1203"}, {"dataset_uid": "000161", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Cruise LMG1205", "url": "https://www.rvdata.us/search/cruise/LMG1205"}], "date_created": "Fri, 20 Dec 2013 00:00:00 GMT", "description": "Since the advent of Antarctic continental glaciation, the opening of the Drake Passage between South America and the Antarctic Peninsula, and the onset of cooling of the Southern Ocean ~40-25 million years ago, evolution of the Antarctic marine biota has been driven by the development of extreme cold temperatures. The biochemical and physiological challenges facing ectotherms living in the Southern Ocean include the reduction of reaction rates and metabolic fluxes and a pervasive weakening of macromolecular interactions. Yet, Southern Ocean ectotherms are now threatened by warming over periods measured in centuries or less. The proposed research seeks to understand the molecular mechanisms of cold adaptation in Antarctic marine fishes and to assess the physiological capacity of these organisms to resist or compensate for rapid oceanic warming. The P.I. will characterize two important and interacting protein systems, the tubulins that form microtubules and the chaperonin CCT (cytoplasmic chaperonin-containing TCP-1, a family of proteins that assists the folding of the tubulins). Higher-level, integrative responses to global temperature change will be analyzed by studying the thermal dependence of cleavage in Antarctic fish embryos (a microtubule-dependent process). The objectives are (1) to determine the contributions of five novel amino acid substitutions found in Antarctic fish beta-tubulins to microtubule assembly at cold temperature. (2) to compare the functional properties of CCT from testis tissues of Antarctic fishes and mammals. (3) to evaluate the effects of increased temperature on embryogenesis in Antarctic fishes. The research will introduce graduate and REU undergraduate students to state-of-the-art biochemical, cellular, and molecular-biological research relevant to ecological and environmental issues of the Antarctic marine ecosystem. The proposed work also will benefit society by developing a cold-functioning chaperonin protein folding system, of great value to the biopharmaceutical and biotechnological industries for use in folding insoluble proteins.", "east": -56.02, "geometry": "POINT(-61.72 -63.75)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -61.2, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Detrich, H. William", "platforms": "Not provided", "repo": "R2R", "repositories": "NCBI GenBank; R2R", "science_programs": null, "south": -66.3, "title": "Microtubule Function, Protein Folding, and Embryogenesis in Antarctic Fishes: An Integrative Approach", "uid": "p0000664", "west": -67.42}, {"awards": "0839007 Near, Thomas", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "Genetic Sequence Data", "datasets": [{"dataset_uid": "000151", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Genetic Sequence Data", "url": "http://www.ncbi.nlm.nih.gov/genbank/"}], "date_created": "Fri, 22 Nov 2013 00:00:00 GMT", "description": "This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).\u003cbr/\u003e\u003cbr/\u003eThe teleost fish fauna in the waters surrounding Antarctica are completely dominated by a single clade of closely related species, the Notothenioidei. This clade offers an unprecedented opportunity to investigate the effects of deep time paleogeographic transformations and periods of global climate change on lineage diversification and facilitation of adaptive radiation. With over 100 species, the Antarctic notothenioid radiation has been the subject of intensive investigation of biochemical, physiological, and morphological adaptations associated with freezing avoidance in the subzero Southern Ocean marine habitats. However, broadly sampled time-calibrated phylogenetic hypotheses of notothenioids have not been used to examine patterns of adaptive radiation in this clade. The goals of this project are to develop an intensive phylogenomic scale dataset for 90 of the 124 recognized notothenioid species, and use this genomic resource to generate time-calibrated molecular phylogenetic trees. The results of pilot phylogenetic studies indicate a very exciting correlation of the initial diversification of notothenioids with the fragmentation of East Gondwana approximately 80 million years ago, and the origin of the Antarctic Clade adaptive radiation at a time of global cooling and formation of polar conditions in the Southern Ocean, approximately 35 million years ago. This project will provide research experiences for undergraduates, training for a graduate student, and support a post doctoral researcher. In addition the project will include three high school students from New Haven Public Schools for summer research internships.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Near, Thomas", "platforms": "Not provided", "repo": "NCBI GenBank", "repositories": "NCBI GenBank", "science_programs": null, "south": -90.0, "title": "Genomic Approaches to Resolving Phylogenies of Antarctic Notothenioid Fishes", "uid": "p0000497", "west": -180.0}, {"awards": "0739783 Junge, Karen", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "Metabolic Activities and Gene Expression of Marine Psychrophiles in Cold Ice", "datasets": [{"dataset_uid": "600083", "doi": "10.15784/600083", "keywords": "Antarctica; Biota; Microbiology; Oceans; Sea Ice; Southern Ocean", "people": "Junge, Karen", "repository": "USAP-DC", "science_program": null, "title": "Metabolic Activities and Gene Expression of Marine Psychrophiles in Cold Ice", "url": "https://www.usap-dc.org/view/dataset/600083"}], "date_created": "Wed, 25 Sep 2013 00:00:00 GMT", "description": "The mechanisms enabling bacteria to be metabolically active at very low temperatures are of considerable importance to polar microbial ecology, astrobiology, climate and cryopreservation. This research program has two main objectives. The first is to investigate metabolic activities and gene expression of polar marine psychrophilic bacteria when confronted with freezing conditions at temperatures above the eutectic of seawater (\u003c54C) to unveil cold adaptation mechanisms with relevance to wintertime sea-ice ecology. The second objective is to discern if psychrophilic processes of leucine incorporation into proteins, shown to occur to -196C, amount to metabolic activity providing for the survival of cells or are merely biochemical reactions still possible in flash-frozen samples without any effect on survival. We will examine extracellular and intracellular processes of psychrophilic activity above and below the eutectic by (i) determining the temperature range of metabolic activities such as DNA synthesis, carbon utilization, respiration and ATP generation using radioactive tracer technology, including a control at liquid helium temperature (-268.9C), (ii) analyzing gene expression in ice using whole genome and microarray analyses and iii) examining the role of exopolymeric substances (EPS) and ice micro-physics for the observed activity using an in-situ microscopy technique. Results of the proposed research can be expected to aid in the determination of cellular and genetic strategies that allow cells to maintain activity at extremely low temperatures within an icy matrix and/or to resume activity again when more growth-permissive conditions are encountered. The research is an interdisciplinary collaboration involving three different institutions with participants in Oceanography, Genomics, and Geophysical Sciences. The proposed activity will support the beginning professional career of a female researcher and will serve as the basis for several undergraduate student laboratory projects.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Junge, Karen", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -90.0, "title": "Metabolic Activities and Gene Expression of Marine Psychrophiles in Cold Ice", "uid": "p0000673", "west": -180.0}, {"awards": "1043564 Karentz, Deneb", "bounds_geometry": null, "dataset_titles": "Expedition data of LMG1106A", "datasets": [{"dataset_uid": "002686", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG1106A", "url": "https://www.rvdata.us/search/cruise/LMG1106A"}], "date_created": "Tue, 17 Jan 2012 00:00:00 GMT", "description": "The proposed research will investigate the genomic basis of the physiological and ecological transition of Antarctic marine phytoplankton from a cold dark winter to a warmer, brighter spring. During a field season at Palmer Station, functional genomics (using next generation sequencing technology to identify expressed genes) and in situ fluorometry (FRRF) will be integrated with classical ecological methods to investigate photosynthetic adaptation during phytoplankton species succession from late winter into spring. Using large data sets, this project will test whether amino acid usages differ based on expression. The specific objectives are (1) To characterize phytoplankton succession from the winter to spring transition, and (2) To correlate community gene expression profiles to adaptational differences among taxa. Broader impacts include training of a post doctoral researcher and two undergraduate science majors, with efforts to attract students from underrepresented groups. The P.I.s also will prepare presentations for the public, regarding research experiences, research results, and the importance of climate change.", "east": null, "geometry": null, "instruments": "EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS", "is_usap_dc": false, "keywords": "R/V LMG", "locations": null, "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Karentz, Deneb", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "R2R", "repositories": "R2R", "science_programs": null, "south": null, "title": "Collaborative Research: Functional Genomics and Physiological Ecology of Seasonal Succession in Antarctic Phytoplankton: Adaptations to Light and Temperature", "uid": "p0000861", "west": null}, {"awards": "0631659 Morgan-Kiss, Rachael; 0631494 Priscu, John", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "GenBank (NCBI) 18S rRNA genes: GU969060 to GU969102, rbcL genes: GU132860 to GU132939; McMurdo Dry Valleys Long-Term Ecological Research (MCM LTER) Program", "datasets": [{"dataset_uid": "000125", "doi": "", "keywords": null, "people": null, "repository": "LTER", "science_program": null, "title": "McMurdo Dry Valleys Long-Term Ecological Research (MCM LTER) Program", "url": "http://www.mcmlter.org/"}, {"dataset_uid": "000126", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "GenBank (NCBI) 18S rRNA genes: GU969060 to GU969102, rbcL genes: GU132860 to GU132939", "url": "http://www.ncbi.nlm.nih.gov/genbank/"}], "date_created": "Fri, 08 Oct 2010 00:00:00 GMT", "description": "Data collected on the permanently ice-covered lakes of the McMurdo Dry Valleys (MCM) during the late 1950\u0027s as part of the International Geophysical Year (IGY) showed that they were the only year round liquid water environments on the continent. Organisms in the lakes must possess novel physiological strategies that allow them to survive at low temperature and under extended darkness. Subsequent research has now shown that most organisms in the lakes are not just \"surviving the extremes\" but are actively feeding, growing and reproducing. However, nearly all research on the MCM lakes is restricted to the austral spring and summer when logistical support is provided. The unique aspects of physiological adaptation and metabolic function during the permanently cold and prolonged darkness of the Antarctic winter remain unknown. As part of the \"International Polar Year 2007-2008\" (IPY), the proposed research will study lakes within the Taylor Valley during the transition to polar night to test the overarching hypothesis that the onset of darkness induces a cascade of physiological changes that alters the functional role of autotrophic and heterotrophic microplankton within the lakes. This overarching theme will be addressed through an interdisciplinary study of selected biological components of the lake ecosystems using genomic and physiological tools to understand not only how individual organisms survive, but how they control ecosystem function during this seasonal transition. \u003cbr/\u003e\u003cbr/\u003eThis project is directly relevant to IPY objectives as it addresses a major identified theme (Adaptations to Life in Extreme Cold and Prolonged Darkness) with an international (UK, NZ),\u003cbr/\u003emultidisciplinary team. The research has substantial broader impacts, as it will add to the body of long-term data accumulated by the MCM LTER and MCM Microbial Observatory projects in a synergistic manner; and it will include three undergraduates, a graduate student and two young female investigators. The project is linked to a highly visible education, outreach and human diversity programs supported by the McMurdo LTER, and initiates new outreach programs, including the Passport to Knowledge program.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Morgan-Kiss, Rachael; Priscu, John; Mikucki, Jill", "platforms": "Not provided", "repo": "LTER", "repositories": "LTER; NCBI GenBank", "science_programs": "LTER", "south": -90.0, "title": "Collaborative Research: IPY- Plankton Dynamics in the McMurdo Dry Valley Lakes During the Transition to Polar Night", "uid": "p0000525", "west": -180.0}, {"awards": "0635470 Detrich, H. William", "bounds_geometry": "POLYGON((-67.41667 -61.2,-66.27667 -61.2,-65.13667 -61.2,-63.99667 -61.2,-62.85667 -61.2,-61.71667 -61.2,-60.57667 -61.2,-59.43667 -61.2,-58.29667 -61.2,-57.15667 -61.2,-56.01667 -61.2,-56.01667 -61.71,-56.01667 -62.22,-56.01667 -62.73,-56.01667 -63.24,-56.01667 -63.75,-56.01667 -64.26,-56.01667 -64.77,-56.01667 -65.28,-56.01667 -65.79,-56.01667 -66.3,-57.15667 -66.3,-58.29667 -66.3,-59.43667 -66.3,-60.57667 -66.3,-61.71667 -66.3,-62.85667 -66.3,-63.99667 -66.3,-65.13667 -66.3,-66.27667 -66.3,-67.41667 -66.3,-67.41667 -65.79,-67.41667 -65.28,-67.41667 -64.77,-67.41667 -64.26,-67.41667 -63.75,-67.41667 -63.24,-67.41667 -62.73,-67.41667 -62.22,-67.41667 -61.71,-67.41667 -61.2))", "dataset_titles": "Expedition Data; Expedition data of LMG1003; Expedition data of LMG1004; Sequence data", "datasets": [{"dataset_uid": "000133", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Sequence data", "url": "http://www.ncbi.nlm.nih.gov/genbank/"}, {"dataset_uid": "001508", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0807"}, {"dataset_uid": "002685", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG1004", "url": "https://www.rvdata.us/search/cruise/LMG1004"}, {"dataset_uid": "002684", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG1003", "url": "https://www.rvdata.us/search/cruise/LMG1003"}, {"dataset_uid": "001509", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0806"}], "date_created": "Mon, 06 Sep 2010 00:00:00 GMT", "description": "Abstract\u003cbr/\u003e\u003cbr/\u003eSince the advent of Antarctic continental glaciation, the opening of the Drake Passage between South America and the Antarctic Peninsula, and the onset of cooling of the Southern Ocean ~38-25 million years ago, evolution of the Antarctic marine biota has been driven by the development of extreme cold temperatures. Because they live at very low and stable temperatures, Antarctic fishes of the suborder Nototheniodei are particularly attractive as models for understanding the mechanisms of biomolecular cold adaptation, or the compensatory restructuring of biochemical and physiological systems to preserve biological function in cold thermal regimes. Two interrelated and potentially co-evolved systems, the tubulins that form microtubules and the chaperonin-containing TCP1 (t-complex protein-1) complex (CCT) that assists the folding of tubulins, provide an unparalleled opportunity to elucidate these mechanisms. This research will yield new and important knowledge regarding: 1) cold adaptation of microtubule assembly and of chaperonin function; and 2) the co-evolutionary origin of tubulin-binding specificity by CCT. The first objective of this proposal is to determine the contributions of five novel amino acid substitutions found in Antarctic fish beta-tubulins to microtubule assembly at cold temperature. The second objective is to establish a chaperonin folding system in vitro using CCT purified from testis tissue of Antarctic fishes and to evaluate its thermal properties and mechanism. The third objective is to evaluate, through phylogenetically controlled contrasts, the hypothesis that CCT and its tubulin substrates from Antarctic fishes have co-evolved to function at cold temperatures. The broader impacts of this proposal include introduction of graduate and REU undergraduate students of Northeastern University to state-of-the-art biochemical, cellular, and molecular-biological research relevant to ecological and environmental issues of the Antarctic marine ecosystem. Because much of the research on the biogenesis and function of cold-adapted proteins will be performed in the field at Palmer Station, these students will gain invaluable experience in the practical considerations of expeditionary biological science. The research also will increase knowledge about molecular cold adaptation in one of the Earth\u0027s extreme environments, and hence is relevant to the formulation of refined hypotheses regarding potential extraterrestrial life on Mars or Europa. The cold-functioning chaperonin protein folding system will be of great value to the biopharmaceutical and biotechnological industries for use in folding insoluble proteins.", "east": -56.01667, "geometry": "POINT(-61.71667 -63.75)", "instruments": "EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ADCP", "is_usap_dc": true, "keywords": "R/V LMG", "locations": null, "north": -61.2, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Detrich, H. William", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "NCBI GenBank", "repositories": "NCBI GenBank; R2R", "science_programs": null, "south": -66.3, "title": "Protein Folding and Function at Cold Temperature: Co-Evolution of the Chaperonin CCT and Tubulins from Antarctic Fishes", "uid": "p0000470", "west": -67.41667}, {"awards": "0649609 Horning, Markus", "bounds_geometry": "POLYGON((165.975 -77.54,166.0631 -77.54,166.1512 -77.54,166.2393 -77.54,166.3274 -77.54,166.4155 -77.54,166.5036 -77.54,166.5917 -77.54,166.6798 -77.54,166.7679 -77.54,166.856 -77.54,166.856 -77.5709,166.856 -77.6018,166.856 -77.6327,166.856 -77.6636,166.856 -77.6945,166.856 -77.7254,166.856 -77.7563,166.856 -77.7872,166.856 -77.8181,166.856 -77.849,166.7679 -77.849,166.6798 -77.849,166.5917 -77.849,166.5036 -77.849,166.4155 -77.849,166.3274 -77.849,166.2393 -77.849,166.1512 -77.849,166.0631 -77.849,165.975 -77.849,165.975 -77.8181,165.975 -77.7872,165.975 -77.7563,165.975 -77.7254,165.975 -77.6945,165.975 -77.6636,165.975 -77.6327,165.975 -77.6018,165.975 -77.5709,165.975 -77.54))", "dataset_titles": "Aging in Weddell Seals: Proximate Mechanisms of Age-Related Changes in Adaptations to Breath-Hold Hunting in an Extreme Environment", "datasets": [{"dataset_uid": "600071", "doi": "10.15784/600071", "keywords": "Antarctica; Biota; McMurdo; Oceans; Seals; Southern Ocean", "people": "Horning, Markus", "repository": "USAP-DC", "science_program": null, "title": "Aging in Weddell Seals: Proximate Mechanisms of Age-Related Changes in Adaptations to Breath-Hold Hunting in an Extreme Environment", "url": "https://www.usap-dc.org/view/dataset/600071"}], "date_created": "Wed, 04 Aug 2010 00:00:00 GMT", "description": "The primary objectives of this research are to investigate the proximate effects of aging on diving capability in the Weddell Seal and to describe mechanisms by which aging may influence foraging ecology, through physiology and behavior. This model pinniped species has been the focus of three decades of research in McMurdo Sound, Antarctica. Compared to the knowledge of pinniped diving physiology and ecology during early development and young adulthood, little is known about individuals nearing the upper limit of their normal reproductive age range. Evolutionary aging theories predict that elderly diving seals should exhibit senescence. This should be exacerbated by surges in the generation of oxygen free radicals via hypoxia-reoxygenation during breath-hold diving and hunting, which are implicated in age-related damage to cellular mitochondria. Surprisingly, limited observations of non-threatened pinniped populations indicate that senescence does not occur to a level where reproductive output is affected. The ability of pinnipeds to avoid apparent senescence raises two major questions: what specific physiological and morphological changes occur with advancing age in pinnipeds and what subtle adjustments are made by these animals to cope with such changes? This investigation will focus on specific, functional physiological and behavioral changes relating to dive capability with advancing age. The investigators will quantify age-related changes in general health and body condition, combined with fine scale assessments of external and internal ability to do work in the form of diving. Specifically, patterns of oxidative status and oxygen use with age will be examined. The effects of age on muscular function, contractile capacity in vascular smooth muscle, and exercise capacity via exercise performance in skeletal muscle will be examined. Data will be compared between Weddell seals in the peak, and near the end, of their reproductive age range. An assessment will be made of the ability to do external work (i.e. diving) as well as muscle functionality (ability to do internal work). The investigators hypothesize that senescence does occur in Weddell seals at the level of small-scale, proximate physiological effects and performance, but that behavioral plasticity allows for a given degree of compensation. Broader impacts include the training of students and outreach activities including interviews and articles written for the popular media. Photographs and project summaries will be available to the interested public on the project website. This study should also establish diving seals as a novel model for the study of cardiovascular and muscular physiology of aging. Research on Weddell seals could validate this model and thus develop a foundation for similar research on other species. Advancement of the understanding of aging by medical science has been impressive in recent years and the development of new models for the study of aging has tremendous potential benefits to society at large", "east": 166.856, "geometry": "POINT(166.4155 -77.6945)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -77.54, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Horning, Markus", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -77.849, "title": "Collaborative Research: Aging in Weddell Seals: Proximate Mechanisms of Age-Related Changes in Adaptations to Breath-Hold Hunting in an Extreme Environment", "uid": "p0000487", "west": 165.975}, {"awards": "0632389 Murray, Alison; 0632278 Ducklow, Hugh", "bounds_geometry": "POLYGON((-77 -62,-75.5 -62,-74 -62,-72.5 -62,-71 -62,-69.5 -62,-68 -62,-66.5 -62,-65 -62,-63.5 -62,-62 -62,-62 -62.7,-62 -63.4,-62 -64.1,-62 -64.8,-62 -65.5,-62 -66.2,-62 -66.9,-62 -67.6,-62 -68.3,-62 -69,-63.5 -69,-65 -69,-66.5 -69,-68 -69,-69.5 -69,-71 -69,-72.5 -69,-74 -69,-75.5 -69,-77 -69,-77 -68.3,-77 -67.6,-77 -66.9,-77 -66.2,-77 -65.5,-77 -64.8,-77 -64.1,-77 -63.4,-77 -62.7,-77 -62))", "dataset_titles": "IPY: Bacterioplankton Genomic Adaptations to Antarctic Winter", "datasets": [{"dataset_uid": "600061", "doi": "10.15784/600061", "keywords": "Antarctica; Antarctic Peninsula; Biota; Chemistry:fluid; Chemistry:Fluid; Oceans; Southern Ocean", "people": "Grzymski, Joseph; Murray, Alison", "repository": "USAP-DC", "science_program": null, "title": "IPY: Bacterioplankton Genomic Adaptations to Antarctic Winter", "url": "https://www.usap-dc.org/view/dataset/600061"}], "date_created": "Thu, 08 Jul 2010 00:00:00 GMT", "description": "The Western Antarctic Peninsula is experiencing one of the most rapid rates of climate warming on Earth, with an increase of 5degrees C in the mean winter temperature in 50 years. Impacts on upper trophic levels are evident, though there have been few, if any studies that have considered the impacts on bacterioplankton in the Southern Ocean. This proposal will characterize the winter bacterioplankton genome, transcriptome, and proteome and discover those features (community composition, genes up-regulated, and proteins expressed) that are essential to winter bacterioplankton survival and livelihood. We have assembled a polar ocean ecology and genomics network including strategic partnerships with Palmer LTER, the British Antarctic Survey\u0027s ocean metagenome program, US and Canadian scientists studying the Arctic Ocean genome, an Australian colleague who specialized in archaeal proteomics, and French colleagues studying Sub-Antarctic and Coastal Adelie Land marine bacterioplankton. The primary objectives of this program are: 1 Describe the differences in diversity and genomic content between austral winter and summer bacterioplankton communities. 2. Investigate the winter-time bacterioplankton growth and cellular signals (mRNA and proteins expressed) in order to understand the specific adaptations key to survival. \u003cbr/\u003e\u003cbr/\u003eOur results will extend from the Antarctic to the Arctic - as the cold, dark, carbon-limited deep seas linking these two systems have many common features. Education and outreach activities target (i) undergraduate and graduate students, hopefully including minority students recruited through the Diversity in Research in Environmental and Marine Sciences (DREAMS) Program at VIMS; (ii) a broad audience with our education and outreach partnerships with The Cousteau Society and with the Census for Antarctic Marine Life program. Data and links to external databases will be listed on the http://genex2.dri.edu website. Sequence data will be publicly accessible in GenBank and IMG-M databases.", "east": -62.0, "geometry": "POINT(-69.5 -65.5)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -62.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Murray, Alison; Grzymski, Joseph; Ducklow, Hugh", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -69.0, "title": "IPY: Bacterioplankton Genomic Adaptations to Antarctic Winter", "uid": "p0000091", "west": -77.0}, {"awards": "0634619 Hammer, William", "bounds_geometry": "POINT(166 -84)", "dataset_titles": "Continued Research on the Jurassic Vertebrate Fauna from the Beardmore Glacier Region of Antarctica", "datasets": [{"dataset_uid": "600062", "doi": "10.15784/600062", "keywords": "Antarctica; Beardmore Glacier; Biota; Geochronology; Solid Earth", "people": "Hammer, William R.", "repository": "USAP-DC", "science_program": null, "title": "Continued Research on the Jurassic Vertebrate Fauna from the Beardmore Glacier Region of Antarctica", "url": "https://www.usap-dc.org/view/dataset/600062"}], "date_created": "Fri, 02 Jul 2010 00:00:00 GMT", "description": "Abstract\u003cbr/\u003e\u003cbr/\u003eThis award supports preparation and study of fossil dinosaurs discovered on Mt. Kirkpatrick, Antarctica, during the 2003-04 field season. The 4,000 pounds of bone bearing matrix to be processed includes new pieces of Cryolophosaurus, a 22 foot long meat eating theropod, as well as a new unnamed sauropod dinosaur and other yet to be identified taxa. This project advances our understanding of dinosaur evolution and adaptation at the beginning of the reign of the dinosaurs, the Late Triassic and Early Jurassic. This period is poorly understood due to lack of fossils, which makes these fossils from Antarctica particularly unique. Also, since these fossils are from high paleolatitudes they will contribute to our understanding of past climates and the physiologic adaptations of dinosaurs to lengthy periods of darkness. \u003cbr/\u003e\u003cbr/\u003eThe broader impacts include outreach to the general public through museum exhibits and presentations.", "east": 166.0, "geometry": "POINT(166 -84)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -84.0, "nsf_funding_programs": "Antarctic Earth Sciences", "paleo_time": null, "persons": "Hammer, William R.", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -84.0, "title": "Continued Research on the Jurassic Vertebrate Fauna from the Beardmore Glacier Region of Antarctica", "uid": "p0000538", "west": 166.0}, {"awards": "0801392 Swanson, Brian", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "Ice Nucleation by Marine Psychrophiles", "datasets": [{"dataset_uid": "600087", "doi": "10.15784/600087", "keywords": "Biota; Microbiology; Oceans; Raman Spectroscopy; Sea Ice; Sea Surface; Southern Ocean", "people": "Swanson, Brian", "repository": "USAP-DC", "science_program": null, "title": "Ice Nucleation by Marine Psychrophiles", "url": "https://www.usap-dc.org/view/dataset/600087"}], "date_created": "Sat, 26 Jun 2010 00:00:00 GMT", "description": "The primary objective of this research is to investigate polar marine psychrophilic bacteria for their potential to nucleate ice using a combination of microbiological, molecular biological and atmospheric science approaches in the laboratory. Very little is known about how psychrophiles interact and cope with ice or their adaptations to conditions of extreme cold and salinity. This work will involve a series of laboratory experiments using a novel freeze-tube technique for assaying freezing spectra which will provide quantitative information on: (i) the temperature-dependent freezing rates for heterogeneously frozen droplets containing sea-ice bacteria, (ii) the proportional occurrence of ice-nucleation activity versus anti-freeze activity among sea-ice bacterial isolates and (iii) the temperature-dependent freezing rates of bacteria with ice-nucleation activity grown at a range of temperatures and salinities. The compound(s) responsible for the observed activity will be identified, which is an essential step towards the development of an in-situ bacterial ice-nucleation detection assay that can be applied in the field to Antarctic water and cloud samples.\u003cbr/\u003e One of the goals of this work is to better understand survival and cold adaptation processes of polar marine bacteria confronted with freezing conditions in sea ice. Since sea ice strongly impacts polar, as well as the global climates, this research is of significant interest because it will also provide data for accessing the importance of bacterial ice nucleation in the formation of sea ice. These measurements of ice-nucleation rates will be the first high-resolution measurements for psychrophilic marine bacteria. Another goal is to better understand the impact of bacterial ice initiation processes in polar clouds by making high-resolution measurements of nucleation rates for cloud bacteria found over Arctic and Antarctic regions. Initial measurements indicate these bacteria nucleate ice at warmer temperatures and the effect in polar regions may be quite important, since ice can strongly impact cloud dynamics, cloud radiative properties, precipitation formation, and cloud chemistry. If these initial measurements are confirmed, the data collected here will be important for improving the understanding of polar cloud processes and models. A third goal is to better understand the molecular basis of marine bacterial ice nucleation by characterizing the ice-nucleation compound and comparing it with those of known plant-derived ice-nucleating bacteria, which are the only ice-nucleating bacteria examined in detail to date. The proposed activity will support the beginning academic career of a post-doctoral researcher and will serve as the basis for several undergraduate student laboratory projects. Results from this research will be widely published in various scientific journals and outreach venues.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Swanson, Brian", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -90.0, "title": "Ice Nucleation by Marine Psychrophiles", "uid": "p0000195", "west": -180.0}, {"awards": "0537143 Blanchette, Robert", "bounds_geometry": "POLYGON((-69 -60,-68.3 -60,-67.6 -60,-66.9 -60,-66.2 -60,-65.5 -60,-64.8 -60,-64.1 -60,-63.4 -60,-62.7 -60,-62 -60,-62 -61,-62 -62,-62 -63,-62 -64,-62 -65,-62 -66,-62 -67,-62 -68,-62 -69,-62 -70,-62.7 -70,-63.4 -70,-64.1 -70,-64.8 -70,-65.5 -70,-66.2 -70,-66.9 -70,-67.6 -70,-68.3 -70,-69 -70,-69 -69,-69 -68,-69 -67,-69 -66,-69 -65,-69 -64,-69 -63,-69 -62,-69 -61,-69 -60))", "dataset_titles": "(Arenz et al. 2006) DQ317323, DQ317324, DQ317325, DQ317326, DQ317327, DQ317328, DQ317329, DQ317330, DQ317331, DQ317332, DQ317333, DQ317334, DQ317335, DQ317336, DQ317337, DQ317338, DQ317339, DQ317340, DQ317341, DQ317342, DQ317343, DQ317344, DQ317345, DQ317346, DQ317347, DQ317348, DQ317349, DQ317350, DQ317351, DQ317352, DQ317353, DQ317354, DQ317355, DQ317356, DQ317357, DQ317358, DQ317359, DQ317360, DQ317361, DQ317362, DQ317363, DQ317364, DQ317365, DQ317366, DQ317367, DQ317368, DQ317369, DQ317370, DQ317371, DQ317372, DQ317373, DQ317374, DQ317375, DQ317376, DQ317377, DQ317378, DQ317379, DQ317380, DQ317381, DQ317382, DQ317383, DQ317384, DQ317385, DQ317386, DQ317387, DQ317388, DQ317389 (Arenz and Blanchette 2009) FJ235934, FJ235935, FJ235936, FJ235937, FJ235938, FJ235939, FJ235940, FJ235941, FJ235942, FJ235943, FJ235944, FJ235945, FJ235946, FJ235947, FJ235948, FJ235949, FJ235950, FJ235951, FJ235952, FJ235953, FJ235954, FJ235955, FJ235956, FJ235957, FJ235958, FJ235959, FJ235960, FJ235961, FJ235962, FJ235963, FJ235964, FJ235965, FJ235966, FJ235967, FJ235968, FJ235969, FJ235970, FJ235971, FJ235972, FJ235973, FJ235974, FJ235975, FJ235976, FJ235977, FJ235978, FJ235979, FJ235980, FJ235981, FJ235982, FJ235983, FJ235984, FJ235985, FJ235986, FJ235987, FJ235988, FJ235989, FJ235990, FJ235991, FJ235992, FJ235993, FJ235994, FJ235995, FJ235996, FJ235997, FJ235998, FJ235999, FJ236000, FJ236001, FJ236002, FJ236003, FJ236004, FJ236005, FJ236006, FJ236007, FJ236008, FJ236009, FJ236010, FJ236011, FJ236012, FJ236013, FJ236014 (Blanchette et al. 2010) GU212367, GU212368, GU212369, GU212370, GU212371, GU212372, GU212373, GU212374, GU212375, GU212376, GU212377, GU212378, GU212379, GU212380, GU212381, GU212382, GU212383, GU212384, GU212385, GU212386, GU212387, GU212388, GU212389, GU212390, GU212391, GU212392, GU212393, GU212394, GU212395, GU212396, GU212397, GU212398, GU212399, GU212400, GU212401, GU212402, GU212403, GU212404, GU212405, GU212406, GU212407, GU212408, GU212409, GU212410, GU212411, GU212412, GU212413, GU212414, GU212415, GU212416, GU212417, GU212418, GU212419, GU212420, GU212421, GU212422, GU212423, GU212424, GU212425, GU212426, GU212427, GU212428, GU212429, GU212430, GU212431, GU212432, GU212433, GU212434", "datasets": [{"dataset_uid": "000121", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "(Arenz et al. 2006) DQ317323, DQ317324, DQ317325, DQ317326, DQ317327, DQ317328, DQ317329, DQ317330, DQ317331, DQ317332, DQ317333, DQ317334, DQ317335, DQ317336, DQ317337, DQ317338, DQ317339, DQ317340, DQ317341, DQ317342, DQ317343, DQ317344, DQ317345, DQ317346, DQ317347, DQ317348, DQ317349, DQ317350, DQ317351, DQ317352, DQ317353, DQ317354, DQ317355, DQ317356, DQ317357, DQ317358, DQ317359, DQ317360, DQ317361, DQ317362, DQ317363, DQ317364, DQ317365, DQ317366, DQ317367, DQ317368, DQ317369, DQ317370, DQ317371, DQ317372, DQ317373, DQ317374, DQ317375, DQ317376, DQ317377, DQ317378, DQ317379, DQ317380, DQ317381, DQ317382, DQ317383, DQ317384, DQ317385, DQ317386, DQ317387, DQ317388, DQ317389 (Arenz and Blanchette 2009) FJ235934, FJ235935, FJ235936, FJ235937, FJ235938, FJ235939, FJ235940, FJ235941, FJ235942, FJ235943, FJ235944, FJ235945, FJ235946, FJ235947, FJ235948, FJ235949, FJ235950, FJ235951, FJ235952, FJ235953, FJ235954, FJ235955, FJ235956, FJ235957, FJ235958, FJ235959, FJ235960, FJ235961, FJ235962, FJ235963, FJ235964, FJ235965, FJ235966, FJ235967, FJ235968, FJ235969, FJ235970, FJ235971, FJ235972, FJ235973, FJ235974, FJ235975, FJ235976, FJ235977, FJ235978, FJ235979, FJ235980, FJ235981, FJ235982, FJ235983, FJ235984, FJ235985, FJ235986, FJ235987, FJ235988, FJ235989, FJ235990, FJ235991, FJ235992, FJ235993, FJ235994, FJ235995, FJ235996, FJ235997, FJ235998, FJ235999, FJ236000, FJ236001, FJ236002, FJ236003, FJ236004, FJ236005, FJ236006, FJ236007, FJ236008, FJ236009, FJ236010, FJ236011, FJ236012, FJ236013, FJ236014 (Blanchette et al. 2010) GU212367, GU212368, GU212369, GU212370, GU212371, GU212372, GU212373, GU212374, GU212375, GU212376, GU212377, GU212378, GU212379, GU212380, GU212381, GU212382, GU212383, GU212384, GU212385, GU212386, GU212387, GU212388, GU212389, GU212390, GU212391, GU212392, GU212393, GU212394, GU212395, GU212396, GU212397, GU212398, GU212399, GU212400, GU212401, GU212402, GU212403, GU212404, GU212405, GU212406, GU212407, GU212408, GU212409, GU212410, GU212411, GU212412, GU212413, GU212414, GU212415, GU212416, GU212417, GU212418, GU212419, GU212420, GU212421, GU212422, GU212423, GU212424, GU212425, GU212426, GU212427, GU212428, GU212429, GU212430, GU212431, GU212432, GU212433, GU212434", "url": "http://www.ncbi.nlm.nih.gov/genbank/"}], "date_created": "Mon, 24 May 2010 00:00:00 GMT", "description": "Fungi in Antarctic ecosystems are major contributors to biodiversity and have great influence on many processes such as biodegradation and nutrient cycling. It is essential for biological surveys as well as genomic and proteomic studies to be completed so a better understanding of these organisms is obtained. Previous research has identified unique fungi associated with historic wooden structures brought to Antarctica by Robert F. Scott and Ernest Shackleton during the Heroic Era of exploration. Many of the fungi found are previously undescribed species that belong to the little known genus Cadophora. The research team will obtain important new information on the fungi present in the Ross Sea and Peninsula Regions of Antarctica, particularly their role in decomposition and nutrient recycling and their mechanisms and strategies for survival in the polar environment. New tools and methods include denaturing gradient gel electrophoresis (DGGE), real-time PCR, and proteomic profiling. These analyses will reveal key details of the physiological adaptations these fungi have evolved to carry out processes such as biodegradation and nutrient cycling under conditions that would inhibit other fungi. This work, coupled with the training and learning opportunities it provides, will be of value to many fields of study including microbial ecology, polar biology, wood microbiology, environmental science, soil science, geobiochemistry, and mycology as well as fungal phylogenetics, proteomics and genomics. Results obtained will have immediate applied use to help preserve and protect Antarctica\u0027s historic monuments. The investigations proposed are a continuation of research to identify the microbes attacking these historic structures and artifacts and to elucidate their biology and ecology in the polar environment. New research will also be done at the historic Cape Adare huts, the first wooden structures to be built in Antarctica and also at East Base, an American historic site on Stonington Island from the Admiral Byrd and Ronne Expeditions of 1939-1948. The research team will conduct vital studies needed to successfully conserve the wooden structures and artifacts at these sites and protect them for future generations", "east": -62.0, "geometry": "POINT(-65.5 -65)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Blanchette, Robert", "platforms": "Not provided", "repo": "NCBI GenBank", "repositories": "NCBI GenBank", "science_programs": null, "south": -70.0, "title": "Studies of Antarctic Fungi: Adaptive Stratigies for Survival and Protecting Antarctica\u0027s Historic Structures", "uid": "p0000187", "west": -69.0}, {"awards": "0636696 DeVries, Arthur", "bounds_geometry": "POLYGON((-68.0025 -52.7599,-67.07254 -52.7599,-66.14258 -52.7599,-65.21262 -52.7599,-64.28266 -52.7599,-63.3527 -52.7599,-62.42274 -52.7599,-61.49278 -52.7599,-60.56282 -52.7599,-59.63286 -52.7599,-58.7029 -52.7599,-58.7029 -53.98242,-58.7029 -55.20494,-58.7029 -56.42746,-58.7029 -57.64998,-58.7029 -58.8725,-58.7029 -60.09502,-58.7029 -61.31754,-58.7029 -62.54006,-58.7029 -63.76258,-58.7029 -64.9851,-59.63286 -64.9851,-60.56282 -64.9851,-61.49278 -64.9851,-62.42274 -64.9851,-63.3527 -64.9851,-64.28266 -64.9851,-65.21262 -64.9851,-66.14258 -64.9851,-67.07254 -64.9851,-68.0025 -64.9851,-68.0025 -63.76258,-68.0025 -62.54006,-68.0025 -61.31754,-68.0025 -60.09502,-68.0025 -58.8725,-68.0025 -57.64998,-68.0025 -56.42746,-68.0025 -55.20494,-68.0025 -53.98242,-68.0025 -52.7599))", "dataset_titles": "Expedition Data; Expedition data of LMG0809; Metadata associated with the description of Akarotaxis gouldae n. sp. (Bathydraconidae)", "datasets": [{"dataset_uid": "601811", "doi": null, "keywords": "Antarctica; Bellingshausen Sea; Cryosphere; Southern Ocean", "people": "Hilton, Eric; Desvignes, Thomas; Corso, Andrew; Cheng, Chi-Hing; Steinberg, Deborah; McDowell, Jan; Biesack, Ellen", "repository": "USAP-DC", "science_program": "LTER", "title": "Metadata associated with the description of Akarotaxis gouldae n. sp. (Bathydraconidae)", "url": "https://www.usap-dc.org/view/dataset/601811"}, {"dataset_uid": "001493", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0810"}, {"dataset_uid": "002728", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0809", "url": "https://www.rvdata.us/search/cruise/LMG0809"}, {"dataset_uid": "001504", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0809"}], "date_created": "Tue, 04 May 2010 00:00:00 GMT", "description": "Antarctic notothenioid fish evolved antifreeze (AF) proteins that prevent ice crystals that enter their body fluids from growing, and thereby avoid freezing in their icy habitats. However, even in the extreme cold Antarctic marine environment, regional gradations of severity are found. The biological correlate for environmental severity in fish is the endogenous ice load, which likely determines the tolerable limit of environmental severity for notothenioid habitation. The endogenous ice load develops from environmental ice crystals entering through body surfaces and somehow localizing to the spleen. How prone the surface tissues are to ice entry, how ice reaches the spleen, and what the fate of splenic ice is, requires elucidation. Spleen sequestration of ice raises the hypothesis that macrophages may play a role in the translocation and perhaps elimination of AF-bound ice crystals. Antifreeze glycoproteins (AFGP) act in concert with a second, recently discovered antifreeze called antifreeze potentiating protein (AFPP), necessitating an assessment of the contribution of AFPP to freezing avoidance. Recent research suggests that the exocrine pancreas and the anterior stomach, not the liver, synthesize AFGPs and secrete them into the intestine, from where they may be returned to the blood. A GI-to-blood transport is a highly unconventional path for a major plasma protein and also begs the questions, What is the source of blood AFPP?. Why are two distinct AF proteins needed and what is the chronology of their evolution? What genomic changes in the DNA are associated with the development or loss of the antifreeze trait? Experiments described in this proposal address these interrelated questions of environmental, organismal, and evolutionary physiology, and will further our understanding of novel vertebrate physiologies, the limits of environmental adaptation, and climatically driven changes in the genome. The proposed research will (1) determine the temporal and spatial heterogeneity of environmental temperature and iciness in progressively more severe fish habitats in the greater McMurdo Sound area, and in the milder Arthur Harbor at Palmer Station. The splenic ice load in fishes inhabiting these sites will be determined to correlate to environmental severity and habitability. (2) Assess the surface tissue site of ice entry and their relative barrier properties in intact fish and isolated tissues preparations (3) Assess the role of immune cells in the fate of endogenous ice, (4) determine whether the blood AFGPs are from intestinal/rectal uptake, (5) examine the contribution of AFPP to the total blood AF activity (6) evaluate the progression of genomic changes in the AFGP locus across Notothenioidei as modulated by disparate thermal environments, in four selected species through the analyses of large insert DNA BAC clones. The origin and evolution of AFPP will be examined also by analyzing BAC clones encompassing the AFPP genomic locus. The broader impacts of the proposed research include training of graduate and undergraduate students in research approaches ranging from physical field measurements to cutting edge genomics. Undergraduate research projects have lead to co-authored publications and will continue to do so. Outreach includes establishing Wiki websites on topics of Antarctic fish biology and freeze avoidance, providing advisory services to the San Francisco Science Exploratorium, and making BAC libraries available to interested polar biologists. This research theme has repeatedly received national and international science news coverage and will continue to be disseminated to the public in that manner.", "east": -58.7029, "geometry": "POINT(-63.3527 -58.8725)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ADCP", "is_usap_dc": false, "keywords": "R/V LMG", "locations": null, "north": -52.7599, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Devries, Arthur", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "USAP-DC", "repositories": "R2R; USAP-DC", "science_programs": null, "south": -64.9851, "title": "Environmental, Organismal and Evolutionary Physiology of Freeze Avoidance in Antarctic Notothenioid Fishes", "uid": "p0000560", "west": -68.0025}, {"awards": "0125890 Sidell, Bruce", "bounds_geometry": "POLYGON((-68.1413 -52.6755,-67.47503 -52.6755,-66.80876 -52.6755,-66.14249 -52.6755,-65.47622 -52.6755,-64.80995 -52.6755,-64.14368 -52.6755,-63.47741 -52.6755,-62.81114 -52.6755,-62.14487 -52.6755,-61.4786 -52.6755,-61.4786 -53.8957,-61.4786 -55.1159,-61.4786 -56.3361,-61.4786 -57.5563,-61.4786 -58.7765,-61.4786 -59.9967,-61.4786 -61.2169,-61.4786 -62.4371,-61.4786 -63.6573,-61.4786 -64.8775,-62.14487 -64.8775,-62.81114 -64.8775,-63.47741 -64.8775,-64.14368 -64.8775,-64.80995 -64.8775,-65.47622 -64.8775,-66.14249 -64.8775,-66.80876 -64.8775,-67.47503 -64.8775,-68.1413 -64.8775,-68.1413 -63.6573,-68.1413 -62.4371,-68.1413 -61.2169,-68.1413 -59.9967,-68.1413 -58.7765,-68.1413 -57.5563,-68.1413 -56.3361,-68.1413 -55.1159,-68.1413 -53.8957,-68.1413 -52.6755))", "dataset_titles": "Expedition Data; Expedition data of LMG0304; Expedition data of LMG0304A", "datasets": [{"dataset_uid": "001596", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0506"}, {"dataset_uid": "002707", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0304A", "url": "https://www.rvdata.us/search/cruise/LMG0304A"}, {"dataset_uid": "001704", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0304"}, {"dataset_uid": "001597", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0505"}, {"dataset_uid": "002706", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0304", "url": "https://www.rvdata.us/search/cruise/LMG0304"}, {"dataset_uid": "002708", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0304A", "url": "https://www.rvdata.us/search/cruise/LMG0304A"}], "date_created": "Tue, 04 May 2010 00:00:00 GMT", "description": "Notothenioid fishes that dominate the fish fauna surrounding Antarctica have been evolving for 10-14 million years at a nearly constant body temperature of ~0C throughout their life histories. As a result, this group of animals is uniquely suited to studies aimed at understanding and identifying features of physiology and biochemistry that result from the process of evolution at cold body temperature. This project has three major objectives aimed at examining adaptations for life in cold environments: \u003cbr/\u003e\u003cbr/\u003e1. Identify the amino acid substitutions in the fatty acid-binding pocket of fatty acyl CoA synthetase (FACS) that explain its substrate specificity. Fatty acids are a major fuel of energy metabolism in Antarctic fishes. FACS catalyzes the condensation of CoASH and fatty acids to fatty acyl CoA esters, a step required for subsequent metabolism of these important compounds. This research may permit us to resolve the specific amino acid substitutions that explain both substrate specificity and preservation of catalytic rate of notothenioid FACS at cold physiological temperatures.\u003cbr/\u003e\u003cbr/\u003e2. Produce a rigorous biochemical and biophysical characterization of the intracellular calcium-binding protein, parvalbumin, from white axial musculature of Antarctic fishes. Parvalbumin plays a pivotal role in facilitating the relaxation phase of fast-contracting muscles and is a likely site of strong selective pressure. Preliminary data strongly indicate that the protein from Antarctic fishes has been modified to ensure function at cold temperature. A suite of physical techniques will be used to determine dissociation constants of Antarctic fish parvalbumins for calcium and magnesium and unidirectional rate constants of ion-dissociation from the protein. Full-length cDNA clones for Antarctic fish parvalbumin(s) will permit deduction of primary amino acid sequence These data will yield insight into structural elements that permit the protein from notothenioid fishes to function at very cold body temperature.\u003cbr/\u003e\u003cbr/\u003e3. Conduct a broad survey of the pattern of cardiac myoglobin expression in the Suborder Notothenoidei. Previous work has indicated a variable pattern of presence or absence of the intracellular oxygen-binding protein, myoglobin (Mb), in hearts of one family of Antarctic notothenioid fishes (Channichthyidae; icefishes). Because Mb is of physiological value in species that express the protein, the observed pattern of interspecific expression has been attributed to unusually low niche competition in the Southern Ocean. This leads to the prediction that similar loss of cardiac Mb should be observed in other notothenioid taxa. This part of the project will survey for the presence and absence of cardiac Mb in as many notothenioid species as possible and, if Mb-lacking species are detected, will extend analyses to determine the mechanism(s) responsible for loss of its expression using molecular biological techniques.", "east": -61.4786, "geometry": "POINT(-64.80995 -58.7765)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PRESSURE/HEIGHT METERS \u003e PRESSURE SENSORS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ADCP", "is_usap_dc": false, "keywords": "R/V LMG", "locations": null, "north": -52.6755, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Sidell, Bruce; Detrich, H. William", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "R2R", "repositories": "R2R", "science_programs": null, "south": -64.8775, "title": "Cold Body Temperature as an Evolutionary Shaping force in the Physiology of Antarctic Fishes", "uid": "p0000241", "west": -68.1413}, {"awards": "0324539 Yen, Jeannette", "bounds_geometry": null, "dataset_titles": "Expedition Data; Expedition data of LMG0308", "datasets": [{"dataset_uid": "001686", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0308"}, {"dataset_uid": "002709", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0308", "url": "https://www.rvdata.us/search/cruise/LMG0308"}], "date_created": "Tue, 04 May 2010 00:00:00 GMT", "description": "This project explores the feasibility of applying fluid physical analyses to evaluate the importance of viscous forces over compensatory temperature adaptations in a polar copepod. The water of the Southern Ocean is 20 Celsius colder and nearly twice as viscous as subtropical seas, and the increased viscosity has significant implications for swimming zooplankton. In each of these warm and cold aquatic environments have evolved abundant carnivorous copepods in the family Euchaetidae. In this exploratory study, two species from the extremes of the natural temperature range (0 and 23C) will be compared to test two alternate hypotheses concerning how Antarctic plankton adapt to the low temperature-high viscosity realm of the Antarctic and to evaluate the importance of viscous forces in the evolution of plankton. How do stronger viscous forces and lower temperature affect the behavior of the Antarctic species? If the Antarctic congener is dynamically similar to its tropical relative, it will operate at the same Reynolds number (Re) as its tropical congener. Alternatively, if the adaptations of the Antarctic congener are proportional to size, they should occupy a higher Re regime, which suggests that the allometry of various processes is not constrained by having to occupy a transitional fluid regime. The experiments are designed with clearly defined outcomes regarding a number of copepod characteristics, such as swimming speed, propulsive force, and size of the sensory field. These characteristics determine not only how copepods relate to the physical world, but also structure their biological interactions. The results of this study will provide insights on major evolutionary forces affecting plankton and provide a means to evaluate the importance of the fluid physical conditions relative to compensatory measures for temperature. Fluid physical, biomechanical, and neurophysiological techniques have not been previously applied to these polar plankton. However, these approaches, if productive and feasible, will provide ways to explore the sensory ecology of polar plankton and the role of small-scale biological-physical-chemical interactions in a polar environment. Experimental evidence validating the importance of viscous effects will also justify further research using latitudinal comparisons of other congeners along a temperature gradient in the world ocean.", "east": null, "geometry": null, "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ADCP", "is_usap_dc": false, "keywords": "R/V LMG", "locations": null, "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Yen, Jeannette", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "R2R", "repositories": "R2R", "science_programs": null, "south": null, "title": "Dynamic Similarity or Size Proportionality? Adaptations of a Polar Copepod.", "uid": "p0000867", "west": null}, {"awards": "0089451 Detrich, H. William", "bounds_geometry": "POLYGON((-70.907 -52.353,-69.8619 -52.353,-68.8168 -52.353,-67.7717 -52.353,-66.7266 -52.353,-65.6815 -52.353,-64.6364 -52.353,-63.5913 -52.353,-62.5462 -52.353,-61.5011 -52.353,-60.456 -52.353,-60.456 -53.64334,-60.456 -54.93368,-60.456 -56.22402,-60.456 -57.51436,-60.456 -58.8047,-60.456 -60.09504,-60.456 -61.38538,-60.456 -62.67572,-60.456 -63.96606,-60.456 -65.2564,-61.5011 -65.2564,-62.5462 -65.2564,-63.5913 -65.2564,-64.6364 -65.2564,-65.6815 -65.2564,-66.7266 -65.2564,-67.7717 -65.2564,-68.8168 -65.2564,-69.8619 -65.2564,-70.907 -65.2564,-70.907 -63.96606,-70.907 -62.67572,-70.907 -61.38538,-70.907 -60.09504,-70.907 -58.8047,-70.907 -57.51436,-70.907 -56.22402,-70.907 -54.93368,-70.907 -53.64334,-70.907 -52.353))", "dataset_titles": "Expedition Data; Expedition data of LMG0304A", "datasets": [{"dataset_uid": "002707", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0304A", "url": "https://www.rvdata.us/search/cruise/LMG0304A"}, {"dataset_uid": "001704", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0304"}, {"dataset_uid": "001869", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0105"}], "date_created": "Tue, 04 May 2010 00:00:00 GMT", "description": "Abstract\u003cbr/\u003eOPP-0089451\u003cbr/\u003eP.I. William Detrich\u003cbr/\u003e\u003cbr/\u003e As the Southern Ocean cooled during the past 25 million years, the fishes of Antarctic coastal waters evolved biochemical and physiological adaptations that maintain essential cellular processes such as cytoskeletal function and gene transcription. Their microtubules, for example, assemble and function at body temperatures (-1.8 to +1 oC) well below those of homeotherms and temperate poikilotherms. The long range goals of the proposed research are to determine, at the molecular level, the adaptations that enhance the assembly of microtubules, the function of kinesin motors, and the expression of globin and tubulin genes. The specific objectives are three: 1) to determine the primary sequence changes and posttranslational modifications that contribute to the efficient polymerization of Antarctic fish tubulins at low temperatures; 2) to evaluate the biochemical adaptations required for efficient function of the brain kinesin motor of Antarctic fishes at low temperatures; and 3) to characterize the structure, organization, and promoter-driven expression of globin and tubulin genes from an Antarctic rockcod (Notothenia coriiceps) and a temperate congener (N. angustata). Brain tubulins from Antarctic fishes differ from those of temperate and warm-blooded vertebrates both in unusual primary sequence substitutions (located primarily in lateral loops and the cores of tubulin monomers) and in posttranslational C-terminal glutamylation. Potential primary sequence adaptations of the Antarctic fish tubulins will be tested directly by production of wild-type and site directed tubulin mutants for functional analysis in vitro. The capacity of mutated and wild-type fish tubulins to form \"cold-stable\" microtubules will be determined by measurement of their critical concentrations for assembly and by analysis of their dynamics by video-enhanced microscopy. Three unusual substitutions in the kinesin motor domain of Chionodraco rastrospinosus may enhance mechanochemical activity at low temperature by modifying the binding of ATP and/or the velocity of the motor. To test the functional significance of these changes, the fish residues will be converted individually, and in concert, to those found in mammalian brain kinesin. Reciprocal substitutions will be introduced into the framework of the mammalian kinesin motor domain. After production in Escherichia coli and purification, the functional performance of the mutant motor domains will be evaluated by measurement of the temperature dependence of their ATPase and motility activities. Molecular adaptation of gene expression in N. coriiceps will be analyzed using an a-globin/b-globin gene pair and an a-tubulin gene cluster. Structural features of N. coriiceps globin and tubulin gene regulatory sequences (promoters and enhancers) that support efficient expression will be assessed by transient transfection assay of promoter/luciferase reporter plasmid constructs in inducible erythrocytic and neuronal model cell systems followed by assay of luciferase reporter activity. Together, these studies should reveal the molecular adaptations of Antarctic fishes that maintain efficient cytoskeletal assembly, mechanochemical motor function, and gene expression at low temperatures. In the broadest sense, this research program should advance the molecular understanding of the poikilothermic mode of life.", "east": -60.456, "geometry": "POINT(-65.6815 -58.8047)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; IN SITU/LABORATORY INSTRUMENTS \u003e PRESSURE/HEIGHT METERS \u003e PRESSURE SENSORS; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ADCP", "is_usap_dc": false, "keywords": "R/V LMG", "locations": null, "north": -52.353, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Sidell, Bruce; Detrich, H. William", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "R2R", "repositories": "R2R", "science_programs": null, "south": -65.2564, "title": "Structure, Function, and Expression of Tubulins, Globins, and Microtubule-Dependent Motors from Cold-Adapted Antarctic Fishes", "uid": "p0000591", "west": -70.907}, {"awards": "9811427 Felbeck, Horst", "bounds_geometry": null, "dataset_titles": "Expedition data of LMG9902", "datasets": [{"dataset_uid": "002716", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG9902", "url": "https://www.rvdata.us/search/cruise/LMG9902"}], "date_created": "Tue, 04 May 2010 00:00:00 GMT", "description": "9811427 Felbeck Deception Island is a flooded caldera in the South Shetland Islands, Antarctica. The most recent eruption was in the 1970s causing the formation of new islands in the caldera and various other structures. It harbors many hot springs and fumaroles submerged in the caldera and intertidally. Sulfide and methane are prominent chemicals in the outflowing waters. Bacterial densities in the caldera reach unusually high values probably due to the input of reduced chemicals as energy sources. The environment around the springs resembles that found at hydrothermal vents where whole communities are based on the input of chemical energy by the hot waters. Similarities to hydrothermal vent environments include cold waters surrounding the hot springs resulting in large distances to the next warm water habitat and a lack of external food sources. The latter is due to ice cover during winter at Deception Island and the large distance to the euphotic zone at the vent sites. These parameters encourage the evolution of alternative ways to support life such as the establishment of a bacterial symbiosis. This Small Grant for Exploratory Research project will focus on an examination of the warm springs around Deception Island for the presence of marine invertebrates with chemoautotrophic symbionts. Maps will be made of any submerged fumaroles and of warm and hot springs in the intertidal zone. If animals are found near the fumaroles or in the hot springs, specimens will be collected. Collected specimens will be examined for the presence of chemoautotrophic bacteria and other adaptations to a hot sulfide- and methane-rich environment using enzyme text experimental incubations to analyze metabolic pathways and microscopic examination.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": false, "keywords": "R/V LMG", "locations": null, "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Smith, Ken", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "R2R", "repositories": "R2R", "science_programs": null, "south": null, "title": "Adaptations of Organisms at the Sulfide- and Methane-Containing Hydrothermal Areas of Deception Island, Antarctica", "uid": "p0000868", "west": null}, {"awards": "0632250 Cary, Stephen", "bounds_geometry": "POLYGON((-38.5 -72.6,-23.963 -72.6,-9.426 -72.6,5.111 -72.6,19.648 -72.6,34.185 -72.6,48.722 -72.6,63.259 -72.6,77.796 -72.6,92.333 -72.6,106.87 -72.6,106.87 -73.185,106.87 -73.77,106.87 -74.355,106.87 -74.94,106.87 -75.525,106.87 -76.11,106.87 -76.695,106.87 -77.28,106.87 -77.865,106.87 -78.45,92.333 -78.45,77.796 -78.45,63.259 -78.45,48.722 -78.45,34.185 -78.45,19.648 -78.45,5.111 -78.45,-9.426 -78.45,-23.963 -78.45,-38.5 -78.45,-38.5 -77.865,-38.5 -77.28,-38.5 -76.695,-38.5 -76.11,-38.5 -75.525,-38.5 -74.94,-38.5 -74.355,-38.5 -73.77,-38.5 -73.185,-38.5 -72.6))", "dataset_titles": "Metagenomic Data Lake Vostok Microbial Community", "datasets": [{"dataset_uid": "000136", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Metagenomic Data Lake Vostok Microbial Community", "url": "http://www.ncbi.nlm.nih.gov/genbank/"}], "date_created": "Fri, 30 Apr 2010 00:00:00 GMT", "description": "This project brings together researchers with expertise in molecular microbial ecology, Antarctic and deep sea environments, and metagenomics to address the overarching question: how do ecosystems dominated by microorganisms adapt to conditions of continuous cold and dark over evolutionarily and geologically relevant time scales? Lake Vostok, buried for at least 15 million years beneath approximately 4 km of ice that has prevented any communication with the external environment for as much as 1.5 million years, is an ideal system to study this question. Water from the lake that has frozen on to the bottom of the ice sheet (accretion ice) is available for study. Several studies have indicated the presence of low abundance, but detectable microbial communities in the accretion ice. Our central hypothesis maintains that Lake Vostok microbes are specifically adapted to life in conditions of extreme cold, dark, and oligotrophy and that signatures of those adaptations can be observed in their genome sequences at the gene, organism, and community levels. To address this hypothesis, we propose to characterize the metagenome (i.e. the genomes of all members of the community) of the accretion ice. using whole genome amplification (WGA), which can provide micrograms of unbiased metagenomic DNA from only a few cells. The results of this project have relevance to evolutionary biology and ecology, subglacial Antarctic lake exploration, biotechnology, and astrobiology. The project directly addresses priorities and themes in the International Polar Year at the national and international levels. A legacy of DNA sequence data and the metagenomic library will be created and maintained. Press releases and a publicly available web page will facilitate communication with the public. K-12 outreach will be the focus of a new, two-tiered program targeting the 7th grade classroom and on site visits to the Joint Genome Institute Production Sequencing Facility by high school juniors and seniors and community college level students. Minority undergraduate researchers will be recruited for research on this project, and support and training are provided to two graduate students, a postdoctoral scholar, and a technician.", "east": 106.87, "geometry": "POINT(34.185 -75.525)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -72.6, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Cary, Stephen", "platforms": "Not provided", "repo": "NCBI GenBank", "repositories": "NCBI GenBank", "science_programs": null, "south": -78.45, "title": "IPY: Collaborative Research: A Metagenomic Investigation of Adaptation to Prolonged Cold and Dark Conditions of the Lake Vostok Microbial Community", "uid": "p0000201", "west": -38.5}, {"awards": "0551969 Moran, Amy", "bounds_geometry": null, "dataset_titles": null, "datasets": null, "date_created": "Tue, 12 Jan 2010 00:00:00 GMT", "description": "This project seeks to understand the evolutionary physiology of reproductive strategies in Southern Ocean marine invertebrates. The fauna of the Southern Ocean has evolved under stable, cold temperatures for approximately 14 million years. These conditions have led to the evolution of unusual physiological and biochemical characteristics, many of which may reflect adaptations to relatively low oxygen availability and high larval oxygen demands. The goal of the proposed projects is to understand latitudinal variation in the function of invertebrate egg masses in relation to oxygen availability and temperature. This relationship is critical to larval survival in the low-temperature, high-oxygen conditions found at high latitudes. In particular, the investigators will: (1) use first principles to model the diffusion of oxygen into egg and embryo masses of Antarctic organisms at environmentally relevant temperatures; (2) test model assumptions by measuring the temperature-dependence of embryonic metabolism and oxygen diffusivity through natural and artificial gels; (3) test model predictions by using oxygen microelectrodes to measure oxygen gradients in both artificial and natural egg masses, and by measuring developmental rates of embryos at different positions in masses; and (4) compare the structure and function of egg masses from the Southern Ocean to those from temperate waters. These components of the study constitute an integrated examination of the evolutionary physiology of egg mass structure and function. Studies of masses endemic to polar conditions will increase the understanding of egg mass evolution across equator-to-pole gradients in temperature and across gradients in oxygen partial pressure. The proposal will support graduate students and will involve several undergraduates in research. The PIs will also design and implement units on polar biology for undergraduate classes at their respective institutions. These educational units will focus on the PIs\u0027 photographs, video footage, experiments, and data from this project. The PIs will use web-linked video and instructional technologies to design and co-teach a new class on polar ecological physiology, will work with local grade school institutions to involve high school students in research, and will develop high school course modules about polar biology.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": false, "keywords": "Not provided", "locations": null, "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Moran, Amy", "platforms": "Not provided", "repositories": null, "science_programs": null, "south": null, "title": "Collaborative Research: Effects of Oxygen and Temperature on Egg Mass Function of Southern Ocean Marine Minvertebrates", "uid": "p0000716", "west": null}, {"awards": "0437887 Sidell, Bruce", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "Differential Expression of Oxygen-binding Proteins in Antarctic Fishes Affects Nitric Oxide-mediated Pathways of Angiogenesis and Mitochondrial Biogenesis; Expedition Data; Expedition data of LMG0705; Expedition data of LMG0706", "datasets": [{"dataset_uid": "002713", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0706", "url": "https://www.rvdata.us/search/cruise/LMG0706"}, {"dataset_uid": "002712", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0705", "url": "https://www.rvdata.us/search/cruise/LMG0705"}, {"dataset_uid": "001534", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0705"}, {"dataset_uid": "600039", "doi": "10.15784/600039", "keywords": "Biota; Oceans; Pot; Sample/collection Description; Sample/Collection Description; Southern Ocean; Trawl", "people": "Sidell, Bruce", "repository": "USAP-DC", "science_program": null, "title": "Differential Expression of Oxygen-binding Proteins in Antarctic Fishes Affects Nitric Oxide-mediated Pathways of Angiogenesis and Mitochondrial Biogenesis", "url": "https://www.usap-dc.org/view/dataset/600039"}], "date_created": "Sun, 06 Dec 2009 00:00:00 GMT", "description": "The polar ocean presently surrounding Antarctica is the coldest, most thermally stable marine environment on earth. Because oxygen solubility in seawater is inversely proportional to temperature, the cold Antarctic seas are an exceptionally oxygen-rich aquatic habitat. Eight families of a single perciform suborder, the Notothenioidei, dominate the present fish fauna surrounding Antarctica. Notothenioids account for approximately 35% of fish species and 90% of fish biomass south of the Antarctic Polar Front. Radiation of closely related notothenioid species thus has occurred rapidly and under a very unusual set of conditions: relative oceanographic isolation from other faunas due to circumpolar currents and deep ocean trenches surrounding the continent, chronically, severely cold water temperatures, very high oxygen availability, very low levels of niche competition in a Southern Ocean depauperate of species subsequent to a dramatic crash in species diversity of fishes that occurred sometime between the mid-Tertiary and present. These features make Antarctic notothenioid fishes an uniquely attractive group for the study of physiological and biochemical adaptations to cold body temperature. \u003cbr/\u003eFew distinctive features of Antarctic fishes are as unique as the pattern of expression of oxygen-binding proteins in one notothenioid family, the Channichthyidae (Antarctic icefishes). All channichthyid icefishes lack the circulating oxygen-binding protein, hemoglobin (Hb); the intracellular oxygen-binding protein, myoglobin (Mb) is not uniformly expressed in species of this family. Both proteins are normally considered essential for adequate delivery of oxygen to aerobically poised tissues of animals. To compensate for the absence of Hb, icefishes have developed large hearts, rapidly circulate a large blood volume and possess elaborate vasculature of larger lumenal diameter than is seen in red-blooded fishes. Loss of Mb expression in oxidative muscles correlates with dramatic elevation in density of mitochondria within the cell, although each individual organelle is less densely packed with respiratory proteins. \u003cbr/\u003eWithin the framework of oxygen movement, the adaptive significance of greater vascular density and mitochondrial populations is understandable but mechanisms underlying development of these characteristics remain unknown. The answer may lie in another major function of both Hb and Mb, degradation of the ubiquitous bioactive compound, nitric oxide (NO). The research will test the hypothesis that loss of hemoprotein expression in icefishes has resulted in an increase in levels of NO that mediate modification of vascular systems and expansion of mitochondrial populations in oxidative tissues. The objectives of the proposal are to quantify the vascular density of retinas in +Hb and -Hb notothenioid species, to characterize NOS isoforms and catalytic activity in retina and cardiac muscle of Antarctic notothenioid fishes, to evaluate level of expression of downstream factors implicated in angiogenesis (in retinal tissue) and mitochondrial biogenesis (in cardiac muscle), and to determine whether inhibition of NOS in vivo results in regression of angiogenic and mitochondrial biogenic responses in icefishes. Broader impacts range from basic biology, through training of young scientists, to enhanced understanding of clinically relevant biomedical processes.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ADCP; IN SITU/LABORATORY INSTRUMENTS \u003e PRESSURE/HEIGHT METERS \u003e PRESSURE SENSORS; IN SITU/LABORATORY INSTRUMENTS \u003e PHOTON/OPTICAL DETECTORS \u003e TURBIDITY METERS", "is_usap_dc": true, "keywords": "R/V LMG; Not provided", "locations": null, "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Sidell, Bruce", "platforms": "Not provided; WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "R2R", "repositories": "R2R; USAP-DC", "science_programs": null, "south": -90.0, "title": "Collaborative Research: Differential Expression of Oxygen-binding Proteins in Antarctic Fishes Affects Nitric Oxide-mediated Pathways of Angiogenesis and Mitochondrial Biogenesis.", "uid": "p0000527", "west": -180.0}, {"awards": "0634682 Kanatous, Shane", "bounds_geometry": "POLYGON((160 -77,160.7 -77,161.4 -77,162.1 -77,162.8 -77,163.5 -77,164.2 -77,164.9 -77,165.6 -77,166.3 -77,167 -77,167 -77.1,167 -77.2,167 -77.3,167 -77.4,167 -77.5,167 -77.6,167 -77.7,167 -77.8,167 -77.9,167 -78,166.3 -78,165.6 -78,164.9 -78,164.2 -78,163.5 -78,162.8 -78,162.1 -78,161.4 -78,160.7 -78,160 -78,160 -77.9,160 -77.8,160 -77.7,160 -77.6,160 -77.5,160 -77.4,160 -77.3,160 -77.2,160 -77.1,160 -77))", "dataset_titles": "The Molecular Signals that Regulate the Ontogeny of Aerobic Capacity, Lipid Metabolism and Elevated Myoglobin Concentrations in the Skeletal Muscles of Weddell Seals", "datasets": [{"dataset_uid": "600063", "doi": "10.15784/600063", "keywords": "Antarctica; Biota; Oceans; Seals; Sea Surface; Southern Ocean", "people": "Kanatous, Shane; Lyons, W. Berry", "repository": "USAP-DC", "science_program": null, "title": "The Molecular Signals that Regulate the Ontogeny of Aerobic Capacity, Lipid Metabolism and Elevated Myoglobin Concentrations in the Skeletal Muscles of Weddell Seals", "url": "https://www.usap-dc.org/view/dataset/600063"}], "date_created": "Fri, 31 Jul 2009 00:00:00 GMT", "description": "During the past three decades, intensive field studies have revealed much about the\u003cbr/\u003ebehavior, physiology, life history, and population dynamics of the Weddell seal (Leptonychotes weddelli) population of McMurdo Sound, Antarctica. These animals are marine predators that are highly adapted for an aquatic life in shore-fast and pack ice habitats. They must locate and capture sparsely distributed under the ice. Most of what is known about their diving behavior is based on studies of adult animals with little known about the development or the genetic controls of diving behavior of young animals. The goal of this project is to examine the temporal development of aerobic capacity, lipid metabolism and oxygen stores in the skeletal muscles of young Weddell seals and to determine which aspects of the cellular environment are important in the regulation of these adaptations during maturation. This project builds on past results to investigate the molecular controls that underlie the development of these adaptations. The first objective is to further characterize the ontogenetic changes in muscle aerobic capacity, lipid metabolism and myoglobin concentration and distribution using enzymatic, immuno-histochemical and myoglobin assays in newly weaned, subadult, and adult seals. The second objective is to determine the molecular controls that regulate these changes in aerobic capacity, fiber type distribution and myoglobin in skeletal muscles during maturation. Through subtractive hybridization and subsequent analysis, differences in mRNA populations in the swimming muscles of the different age classes of Weddell seals will be determined. These techniques will allow for the identification of the proteins and transcription factors that influence the ontogenetic changes in myoglobin concentration, fiber type distribution and aerobic capacity. These results will increase our\u003cbr/\u003eunderstanding of both the ontogeny and molecular mechanisms by which young seals acquire the physiological capabilities to make deep (up to 700 m) and long aerobic dives (ca 20 min). This study will advance knowledge of the molecular regulation for the\u003cbr/\u003eadaptations that enable active skeletal muscle to function under hypoxic conditions; this has a broader application for human medicine especially in regards to cardiac and pulmonary disease. Additional broader impacts include the participation of underrepresented scientists and a continuation of a website in collaboration\u003cbr/\u003ewith the Science Teachers Access to Resources at Southwestern University (STARS Program) which involves weekly updates about research efforts during the field season, weekly questions/answer session involving students and teachers, and updates on research results throughout the year.", "east": 167.0, "geometry": "POINT(163.5 -77.5)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -77.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Kanatous, Shane; Lyons, W. Berry", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -78.0, "title": "The Molecular Signals that Regulate the Ontogeny of Aerobic Capacity, Lipid Metabolism and Elevated Myoglobin Concentrations in the Skeletal Muscles of Weddell Seals", "uid": "p0000536", "west": 160.0}, {"awards": "0238281 Marsh, Adam", "bounds_geometry": "POLYGON((163 -77,163.4 -77,163.8 -77,164.2 -77,164.6 -77,165 -77,165.4 -77,165.8 -77,166.2 -77,166.6 -77,167 -77,167 -77.1,167 -77.2,167 -77.3,167 -77.4,167 -77.5,167 -77.6,167 -77.7,167 -77.8,167 -77.9,167 -78,166.6 -78,166.2 -78,165.8 -78,165.4 -78,165 -78,164.6 -78,164.2 -78,163.8 -78,163.4 -78,163 -78,163 -77.9,163 -77.8,163 -77.7,163 -77.6,163 -77.5,163 -77.4,163 -77.3,163 -77.2,163 -77.1,163 -77))", "dataset_titles": "Marine Invertebrates of McMurdo Sound", "datasets": [{"dataset_uid": "600034", "doi": "10.15784/600034", "keywords": "Antarctica; Biota; McMurdo Sound; Oceans; Photo/video; Photo/Video; Southern Ocean", "people": "Marsh, Adam G.", "repository": "USAP-DC", "science_program": null, "title": "Marine Invertebrates of McMurdo Sound", "url": "https://www.usap-dc.org/view/dataset/600034"}], "date_created": "Mon, 09 Jun 2008 00:00:00 GMT", "description": "Although the cold ocean ecosystems comprise seventy-two percent of the biosphere on Earth by volume, they remain sparsely inhabited and relatively unexploited, particularly in terms of metazoan phyla. Consequently, the few animals that can exist at this border of intracellular freezing represent ideal systems for exploring genomic-level processes of environmental adaptations. Understanding life at a margin of the biosphere is likely to convey significant insights into the essential genomic processes necessary for survival under intense selection pressures. This study of adaptive mechanisms in genomic networks focuses on an experimental system that faces a formidable challenge for viability at low water temperatures: embryonic development at sea water temperatures of -1.8 o C in two Antarctic echinoderms, the sea star Odontaster validus and the sea urchin Sterechinus neumayeri. The project strategy will quantify temperature effects on gene expression and protein turnover networks during early development using a Bayesian network analysis to identify clusters of genes and proteins whose expression levels are associated in fixed, synergistic interactions. Ultimately, there is a simple question to be addressed: Is it more or less difficult (complex) for an embryo to develop in an extreme environment? To answer this question, the research plan will decipher network topologies and subnet structuring to uncover gene connectivity patterns associated with embryo development in this polar environment. This is the new area of Environmental Genomics that the PI will explore by expanding his research experience into computational network analyses. Overall, there is a significant need for integrative biologists in the future development of environmental sciences, particularly for the application of genomic-scale technologies to answer ecological-scale questions. The educational goals of this CAREER proposal are focused at two levels in terms of interesting young students in the developing field of environmental genomics: 1) increasing the racial diversity of the scientists attracted to environmental research, and 2) increasing the awareness of career opportunities within environmental research.\u003cbr/\u003eThese educational objectives are incorporated into the research plan to engage students with the excitement of working in an extreme environment such as Antarctica and to interest them in the insights that genome-level research can reveal about how organisms are adapted to specific habitats. Working in a remote, extreme environment such as Antarctica is always a challenge. However, the adventurous nature of the work can be utilized to establish educational and outreach components of high interest to both undergraduate students and the public in general. The proposed plan will bring the experience of working in Antarctica to a larger audience through several means. These include the following: the project theme of environmental genomics will be incorporated into a new Bioinformatics curriculum currently being developed at the University of Delaware; an intern program will be implemented to involved minority undergraduate students in summer research in the United States and then to bring the students to Antarctica to participate in the research; and a K-12 education program will bring the excitement of working in Antarctica to the classrooms of thousands of children (U.S. and international) through a program produced with the Marine Science Public Education Office at the University of Delaware.", "east": 167.0, "geometry": "POINT(165 -77.5)", "instruments": null, "is_usap_dc": true, "keywords": "FIELD SURVEYS", "locations": null, "north": -77.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Marsh, Adam G.", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -78.0, "title": "CAREER: Genomic Networks for Cold-Adaptation in Embryos of Polar Marine Invertebrates", "uid": "p0000240", "west": 163.0}, {"awards": "0337656 Lee, Richard", "bounds_geometry": "POLYGON((-64.1 -64.75,-64.085 -64.75,-64.07 -64.75,-64.055 -64.75,-64.04 -64.75,-64.025 -64.75,-64.01 -64.75,-63.995 -64.75,-63.98 -64.75,-63.965 -64.75,-63.95 -64.75,-63.95 -64.757,-63.95 -64.764,-63.95 -64.771,-63.95 -64.778,-63.95 -64.785,-63.95 -64.792,-63.95 -64.799,-63.95 -64.806,-63.95 -64.813,-63.95 -64.82,-63.965 -64.82,-63.98 -64.82,-63.995 -64.82,-64.01 -64.82,-64.025 -64.82,-64.04 -64.82,-64.055 -64.82,-64.07 -64.82,-64.085 -64.82,-64.1 -64.82,-64.1 -64.813,-64.1 -64.806,-64.1 -64.799,-64.1 -64.792,-64.1 -64.785,-64.1 -64.778,-64.1 -64.771,-64.1 -64.764,-64.1 -64.757,-64.1 -64.75))", "dataset_titles": null, "datasets": null, "date_created": "Fri, 06 Jun 2008 00:00:00 GMT", "description": "Polar terrestrial environments are often described as deserts, where water availability is recognized as one of the most important limits on the distribution of terrestrial organisms. In addition, prolonged low winter temperatures threaten survival, and summer temperatures challenge organisms with extensive diel variations and rapid transitions from freezing to desiccating conditions. Global warming has further impacted the extreme thermal and hydric conditions experienced by Antarctic terrestrial plant and arthropod communities, especially as a result of glacial retreat along the Antarctic Peninsula. This research will focus on thermal and hydric adaptations in the terrestrial midge, Belgica antarctica, the largest and most southerly holometabolous insect living in this challenging and changing environment. \u003cbr/\u003eOverwintering midge larvae encased in the frozen substrate must endure desert-like conditions for more than 300 days since free water is biologically unavailable as ice. During the summer, larvae may be immersed in melt water or outwash from penguin colonies and seal wallows, in addition to saltwater splash. Alternatively, the larvae may be subjected to extended periods of desiccation as their microhabitats dry out. Due to their small size, relative immobility and the patchiness of suitable microhabitats, larvae may thus be subjected to stresses that include desiccation, hypo- or hyperosmotic conditions, high salinity exposure, and anoxia for extended periods. Research efforts will focus in three areas relevant to the stress tolerance mechanisms operating in these midges:(1) obtaining a detailed characterization of microclimatic conditions experienced by B. antarctica, especially those related to thermal and hydric diversity, both seasonally and among microhabitat types in the vicinity of Palmer Station, Antarctica; (2) examining the effects of extreme fluctuations in water availability and effects on physiological and molecular responses - to determine if midge larvae utilize the mechanism of cryoprotective dehydration for winter survival, and if genes encoding heat shock proteins and other genes are upregulated in larval responses to dehydration and rehydration; (3) investigating the dietary transmission of cryoprotectants from plant to insect host, which will test the hypothesis that midge larvae acquire increased resistance to desiccation and temperature stress by acquiring cryoprotectants from their host plants. \u003cbr/\u003eThis project will provide outreach to both elementary and secondary educators and their students. The team will include a teacher who will benefit professionally by full participation in the research, and will also assist in providing outreach to other teachers and their students. From Palmer Station, the field team will communicate daily research progress by e-mail supplemented with digital pictures with teachers and their elementary students to stimulate interest in an Antarctic biology and scientific research. These efforts will be supplemented with presentations at local schools and national teacher meetings, and by publishing hands-on, inquiry-based articles related to cryobiology and polar biology in education journals. Furthermore, the principal investigators will maintain major commitments to training graduate students and postdoctoral scholars, as well as undergraduate students by providing extended research experience that includes publication of scientific papers and presentations at national meetings.", "east": -63.95, "geometry": "POINT(-64.025 -64.785)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -64.75, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Denlinger, David; Lee, Richard", "platforms": "Not provided", "repositories": null, "science_programs": null, "south": -64.82, "title": "Physiological and Molecular Mechanisms of Stress Tolerance in a Polar Insect", "uid": "p0000742", "west": -64.1}]

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