USAP-DC

{"dp_type": "Project", "free_text": "DNA"}

[{"awards": "2228257 Michaud, Alexander", "bounds_geometry": "POINT(-112.05 -79.28)", "dataset_titles": null, "datasets": null, "date_created": "Wed, 31 May 2023 00:00:00 GMT", "description": "The goals of this work are to determine the taxonomic identity of viable and preserved microbial cells, and decode the genetic repertoire that confers survival of burial and long-term viability within glacial ice. We will achieve these goals by utilizing subsamples from the ~65 ka record of the West Antarctic Ice Sheet Divide (WD) Ice Core. WD samples will be melted using the Desert Research Institute\u2019s ice core melting system that is optimized for glaciobiological sampling. Microbial cells from the meltwater will be sorted using fluorescence-activated cell sorting, and individually sorted cells will have their genomes sequenced. The fluorescence-based methods will discern the viable (metabolically active) cells from those cells that are non-viable but preserved in the ice (DNA-containing). Our genomic analysis will identify the taxonomy of each cell, presence of known genes that confer survival in permanently frozen environments, and comparatively analyze genomes to determine the core set of genes required by viable cells to persist in an ice sheet. Accomplishing these goals contains significant risk because microbial cells within the ice sheet may have damaged membranes and DNA, rendering their genomes inadequate for sequencing. However, existing methods to study ice core biology cannot produce results with the low-biomass and small sample volumes from ice coring projects. While there are unknowns surrounding the suitability of the cells for flow cytometric sorting and single cell sequencing, making this project an exploratory endeavor; it will be a transformative step toward understanding the ecology of one of the most understudied environments on Earth.", "east": -112.05, "geometry": "POINT(-112.05 -79.28)", "instruments": null, "is_usap_dc": true, "keywords": "WAIS divide; ICE SHEETS; BACTERIA/ARCHAEA; ICE CORE RECORDS; TERRESTRIAL ECOSYSTEMS", "locations": "WAIS divide", "north": -79.28, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Michaud, Alexander; Winski, Dominic A.", "platforms": null, "repositories": null, "science_programs": null, "south": -79.28, "title": "EAGER: ANT LIA: Persist or Perish: Records of Microbial Survival and Long-term Persistence from the West Antarctic Ice Sheet", "uid": "p0010421", "west": -112.05}, {"awards": "1744767 Sanders, Robert", "bounds_geometry": "POLYGON((-68 -64,-67.4 -64,-66.8 -64,-66.2 -64,-65.6 -64,-65 -64,-64.4 -64,-63.8 -64,-63.2 -64,-62.6 -64,-62 -64,-62 -64.5,-62 -65,-62 -65.5,-62 -66,-62 -66.5,-62 -67,-62 -67.5,-62 -68,-62 -68.5,-62 -69,-62.6 -69,-63.2 -69,-63.8 -69,-64.4 -69,-65 -69,-65.6 -69,-66.2 -69,-66.8 -69,-67.4 -69,-68 -69,-68 -68.5,-68 -68,-68 -67.5,-68 -67,-68 -66.5,-68 -66,-68 -65.5,-68 -65,-68 -64.5,-68 -64))", "dataset_titles": "Companion datasets to Diversity of microbial eukaryotes along the West Antarctic peninsula in austral spring.; Expedition Data of NBP1910; NBP1910_protist_community_RNA Raw sequence reads", "datasets": [{"dataset_uid": "200320", "doi": "10.6084/m9.figshare.19514110.v3", "keywords": null, "people": null, "repository": "figshare", "science_program": null, "title": "Companion datasets to Diversity of microbial eukaryotes along the West Antarctic peninsula in austral spring.", "url": "https://doi.org/10.6084/m9.figshare.19514110.v3"}, {"dataset_uid": "200319", "doi": "", "keywords": null, "people": null, "repository": "NCBI", "science_program": null, "title": "NBP1910_protist_community_RNA Raw sequence reads", "url": "https://www.ncbi.nlm.nih.gov/search/all/?term=PRJNA807326"}, {"dataset_uid": "200325", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data of NBP1910", "url": "https://www.rvdata.us/search/cruise/NBP1910"}], "date_created": "Wed, 27 Jul 2022 00:00:00 GMT", "description": "Traditional models of oceanic food chains have consisted of photosynthetic algae (phytoplankton) being ingested by small animals (zooplankton), which were ingested by larger animals (fish). These traditional models changed as new methods allowed recognition of the importance of bacteria and other non-photosynthetic protozoa in more complex food webs. More recently, the wide-spread existence of mixotrophs (organisms that can both photosynthesize and ingest food particles) and their importance as microbial predators has been recognized in many oceanographic areas. In the Southern Ocean, the only two surveys of mixotrophs have suggested that there may be seasonal differences in their importance as predators. During the long polar night (winter), the ability of mixotrophs to ingest particulate food may aid in their survival thus ensuring a sufficient population in spring to support a phytoplankton bloom once photosynthesis rates can increase. Thus mixotrophs may provide a critical early food source upon which zooplankton and larger animals depend on for growth and reproduction. This project will advance understanding of mixotroph diversity and their ecological impact within the Southern Ocean microbial food web. Specifically, efforts will be focused on mixotrophy in the western Antarctica peninsula region during the austral spring and autumn when there are likely to be changes in the relative importance of photosynthesis and ingestion to mixotrophs. The project will provide research opportunities for undergraduate and graduate students and a post-doctoral researcher. There will be real-time outreach from the Southern Ocean to the public via blogs and interviews, and to high school art students through an established program that blends science and art education. Despite traditional views of protists as either \"phototrophic\" or \"heterotrophic,\" there are many photosynthetic protists that consume prey (mixotrophy). Mixotrophy is a widespread phenomenon in aquatic systems and phytoplankton groups with known mixotrophic species, notably chrysophytes, cryptophytes, prymnesiophytes, prasinophytes and dinoflagellates, are present and often abundant in Antarctic waters. However, in the Southern Ocean, the presence of mixotrophic phytoflagellates has been surveyed only twice prior to this project: in the Ross Sea during Austral spring 2008 and summer 2011. The primary goals of the project are to gain better understanding of mixotroph diversity and their ecological impact with respect to the Southern Ocean microbial food web. The contribution of mixotrophs to primary production and bacterial consumption is likely linked to the taxonomic composition of the community and the abundance of particular species. Abundances of novel mixotrophic species will be evaluated via qPCR, which will be coupled with assessments of rates of feeding and photosynthesis with the goal of describing how active mixotrophs direct the movement of carbon through food webs. These experiments will help the determination of how viable and widespread mixotrophy is as a nutritional strategy in polar waters and give direct information on the currently unknown diversity of mixotrophic taxa under different environmental conditions occurring in austral spring and autumn. Furthermore, the methods will simultaneously yield information on the whole communities of protists - mixotrophic, phototrophic and heterotrophic. In addition, a method to examine aspects of the taxonomic and functional diversities of the bacterivorous/mixotrophic community will be employed. A thymidine analog (BrdU) will be used to label DNA of eukaryotes feeding on bacteria. The BrdU-labeled eukaryotic DNA will be isolated using immunoprecipitation. High-throughput sequencing of the labeled DNA (bacterivores) versus unlabeled community DNA will determine the diversity of bacterivorous mixotrophs relative to other microeukaryotes. Flow cytometric sorting based on chlorophyll to focus on mixotrophic species. These approaches will elucidate a gap in current knowledge of the influence of microbial interactions in the Southern Ocean under different conditions. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": -62.0, "geometry": "POINT(-65 -66.5)", "instruments": null, "is_usap_dc": true, "keywords": "Antarctic Peninsula; PLANKTON; COASTAL", "locations": "Antarctic Peninsula", "north": -64.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Sanders, Robert; Gast, Rebecca; Jeffrey, Wade H.", "platforms": null, "repo": "figshare", "repositories": "Other", "science_programs": null, "south": -69.0, "title": "Collaborative Research: Diversity and ecological impacts of Antarctic mixotrophic phytoplankton", "uid": "p0010357", "west": -68.0}, {"awards": "2032029 Gerken, Sarah", "bounds_geometry": null, "dataset_titles": null, "datasets": null, "date_created": "Mon, 13 Jun 2022 00:00:00 GMT", "description": "Ocean communities play an important role in determining the natural and human impacts of global change. The most conspicuous members of those communities are generally large vertebrates such as marine mammals and sea birds. But smaller animals often determine how the changes impact those charismatic animals. In the Antarctic, where some of the most dramatic physical changes are taking place, we do not know much about what small animals exist. This project will sample the sub-Antarctic and three different Antarctic seas with a hope of identifying, quantifying and discovering the variation in species of a group of small invertebrates. Comma shrimp, also called cumaceans, are rarely seen elsewhere but may be common and important in the communities of these locations. Antarctic sampling traditionally used gear that was not very effective at catching cumaceans so we do not know what species exist there and how common they are. This study will utilize modern sampling methods that will allow comma shrimp to be sampled. This will lead to discoveries about the diversity and abundance of comma shrimp, as well as their relationship to other invertebrate species. Major impacts of this work will be an enhancement of museum collections, the development of description of all the comma shrimp of Antarctica including new and unnamed species. Those contributions may be especially important as we strive to understand what drives the dynamics of charismatic vertebrates and fisheries that are tied to Antarctic food webs. \u003cbr/\u003e\u003cbr/\u003eThis project will collect cumaceans from benthic samples from the Antarctic peninsula, Bransfield Strait, and the Weddell Sea using benthic sleds, boxcores and megacores. Specimens will be fixed in 95% ethanol, preserved in 95% ethanol and 5% glycerin to preserve both morphology and DNA, and some specimens will be partially or wholly preserved in RNALater to preserve RNA and DNA. The specimens will form the basis for a monograph synthesizing current knowledge on the Subantarctic and Antarctic Cumacea, including diagnoses of all species, descriptions of new species, additional description for currently unknown life stages of known species, and vouchered gene sequences for all species collected. The monograph will include keys to all families, genera and species known from the region. Monographic revisions that include identification resources are typically useful for decades to a broad spectrum of other scientists.\u003cbr/\u003e\u003cbr/\u003eThis award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "AMD/US; AMD; USAP-DC; NSF/USA; ANIMALS/INVERTEBRATES; Weddell Sea; SHIPS; Antarctic Peninsula", "locations": "Antarctic Peninsula; Weddell Sea", "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Gerken, Sarah", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e SHIPS", "repositories": null, "science_programs": null, "south": null, "title": "RAPID: Monographing the Antarctic and Subantarctic Cumacea", "uid": "p0010338", "west": null}, {"awards": "2053726 Hofmann, Gretchen", "bounds_geometry": "POLYGON((163 -77,163.4 -77,163.8 -77,164.2 -77,164.6 -77,165 -77,165.4 -77,165.8 -77,166.2 -77,166.6 -77,167 -77,167 -77.1,167 -77.2,167 -77.3,167 -77.4,167 -77.5,167 -77.6,167 -77.7,167 -77.8,167 -77.9,167 -78,166.6 -78,166.2 -78,165.8 -78,165.4 -78,165 -78,164.6 -78,164.2 -78,163.8 -78,163.4 -78,163 -78,163 -77.9,163 -77.8,163 -77.7,163 -77.6,163 -77.5,163 -77.4,163 -77.3,163 -77.2,163 -77.1,163 -77))", "dataset_titles": "Analyses combining ATAC-seq, RRBS, and RNA-seq data for purple urchins", "datasets": [{"dataset_uid": "200288", "doi": "", "keywords": null, "people": null, "repository": "github", "science_program": null, "title": "Analyses combining ATAC-seq, RRBS, and RNA-seq data for purple urchins", "url": "https://github.com/snbogan/Sp_RRBS_ATAC"}], "date_created": "Thu, 14 Apr 2022 00:00:00 GMT", "description": "Part 1: Non-technical description:\r\n\tWith support from the Office of Polar Programs, this project will evaluate how an important part of the food web in the coastal ocean of Antarctica will respond to climate change. The focal study organism in the plankton is a shelled mollusk, the Antarctic pteropod, Limacina helicina antarctica, a Southern Ocean organism that this known to respond to climate driven changes in ocean acidification and ocean warming. Ocean acidification, the lowering of ocean pH via the absorption of atmospheric carbon dioxide in the surface of the ocean, is a change in the ocean that is expected to cross deleterious thresholds of pH within decades. This study will improve understanding of how pteropods will respond, which will provide insight into predicting the resilience of the Antarctic marine ecosystem during future changes, one of the planets last marine wildernesses. The project will use tools of molecular biology to examine specifically how gene expression is modulated in the pteropods, and further, how the changes and regulation of genes act to resist the stress of low pH and high temperature. In addition, this project supports the training of Ph.D. graduate students and advances the goal of inclusive excellence in STEM and in marine sciences, in particular. The students involved in this project are from groups traditionally under-represented in marine science including first-generation college students. Overall, the project contributes to the development of the U.S. work force and contributes to diversity and inclusive excellence in the geosciences.\r\n\r\nPart 2: Technical description: \r\nThe overarching goal of this project is to investigate the molecular response of the Antarctic thecosome pteropod, Limacina helicina antarctica to ocean acidification (OA) and ocean warming. The project will investigate changes in the epigenome of juvenile L. h. antarctica, by assessing the dynamics of DNA methylation in response to three scenarios of environmental conditions that were simulated in laboratory mesocosm CO2 experiments: (1) present-day pCO2 conditions for summer and winter, (2) future ocean acidification expected within 10-15 years, and (3) a multiple stressor experiment to investigate synergistic interaction of OA and high temperature stress. Recent lab-based mesocosm experiment research showed significant changes in the dynamics of global DNA methylation in the pteropod genome, along with variation in gene expression in response to abiotic changes. Thus, it is clear that juvenile L. h. antarctica are capable of mounting a substantial epigenetic response to ocean acidification. However, it is not known how DNA methylation, as an epigenetic process, is modulating changes in the transcriptome. In order to address this gap in the epigenetic knowledge regarding pteropods, the project will use next-generation sequencing approaches (e.g., RNA sequencing and reduced representation bisulfite sequencing) to integrate changes in methylation status with changes in gene expression in juvenile pteropods. Overall, this investigation is an important step in exploring environmental transcriptomics and phenotypic plasticity of an ecologically important member of Southern Ocean macrozoooplankton in response to anthropogenic climate change.\r\n\r\nThis award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 167.0, "geometry": "POINT(165 -77.5)", "instruments": null, "is_usap_dc": true, "keywords": "AMD; USAP-DC; McMurdo Sound; FIELD INVESTIGATION; AMD/US; MARINE ECOSYSTEMS; USA/NSF; ANIMALS/INVERTEBRATES", "locations": "McMurdo Sound", "north": -77.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Hofmann, Gretchen", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION", "repo": "github", "repositories": "Other", "science_programs": null, "south": -78.0, "title": "The Role of the Epigenetic Mechanism, DNA Methylation, in the Tolerance and Resistance of Antarctic Pteropods to Ocean Acidification and Warming", "uid": "p0010313", "west": 163.0}, {"awards": "1643534 Cassar, Nicolas", "bounds_geometry": "POLYGON((-83 -62,-80.3 -62,-77.6 -62,-74.9 -62,-72.2 -62,-69.5 -62,-66.8 -62,-64.1 -62,-61.4 -62,-58.7 -62,-56 -62,-56 -63.1,-56 -64.2,-56 -65.3,-56 -66.4,-56 -67.5,-56 -68.6,-56 -69.7,-56 -70.8,-56 -71.9,-56 -73,-58.7 -73,-61.4 -73,-64.1 -73,-66.8 -73,-69.5 -73,-72.2 -73,-74.9 -73,-77.6 -73,-80.3 -73,-83 -73,-83 -71.9,-83 -70.8,-83 -69.7,-83 -68.6,-83 -67.5,-83 -66.4,-83 -65.3,-83 -64.2,-83 -63.1,-83 -62))", "dataset_titles": "Palmer LTER 18S rRNA gene metabarcodin; rDNA amplicon sequencing of WAP microbial community", "datasets": [{"dataset_uid": "200285", "doi": "", "keywords": null, "people": null, "repository": "NCBI", "science_program": null, "title": "Palmer LTER 18S rRNA gene metabarcodin", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA508517"}, {"dataset_uid": "200286", "doi": "", "keywords": null, "people": null, "repository": "NCBI", "science_program": null, "title": "rDNA amplicon sequencing of WAP microbial community", "url": "https://www.ncbi.nlm.nih.gov/sra/SRR6162326/"}], "date_created": "Thu, 03 Mar 2022 00:00:00 GMT", "description": "This project seeks to make detailed measurements of the oxygen content of the surface ocean along the Western Antarctic Peninsula. Detailed maps of changes in net oxygen content will be combined with measurements of the surface water chemistry and phytoplankton distributions. The project will determine the extent to which on-shore or offshore phytoplankton blooms along the peninsula are likely to lead to different amounts of carbon being exported to the deeper ocean. \r\n\r\nThe project will analyze oxygen in relation to argon that will allow determination of the physical and biological contributions to surface ocean oxygen dynamics. These assessments will be combined with spatial and temporal distributions of nutrients (iron and macronutrients) and irradiances. This will allow the investigators to unravel the complex interplay between ice dynamics, iron and physical mixing dynamics as they relate to Net Community Production (NCP) in the region. NCP measurements will be normalized to Particulate Organic Carbon (POC) and be used to help identify area of \"High Biomass and Low NCP\" and those with \"Low Biomass and High NCP\" as a function of microbial plankton community composition. The team will use machine learning methods- including decision tree assemblages and genetic programming- to identify plankton groups key to facilitating biological carbon fluxes. Decomposing the oxygen signal along the West Antarctic Peninsula will also help elucidate biotic and abiotic drivers of the O2 saturation to further contextualize the growing inventory of oxygen measurements (e.g. by Argo floats) throughout the global oceans.", "east": -56.0, "geometry": "POINT(-69.5 -67.5)", "instruments": null, "is_usap_dc": true, "keywords": "West Antarctica; AMD; USA/NSF; LABORATORY; AMD/US; USAP-DC; BIOGEOCHEMICAL CYCLES", "locations": "West Antarctica", "north": -62.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Cassar, Nicolas", "platforms": "OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repo": "NCBI", "repositories": "Other", "science_programs": null, "south": -73.0, "title": "Biological and Physical Drivers of Oxygen Saturation and Net Community Production Variability along the Western Antarctic Peninsula", "uid": "p0010303", "west": -83.0}, {"awards": "1644155 Twining, Benjamin", "bounds_geometry": "POLYGON((78 -68.4,78.05 -68.4,78.1 -68.4,78.15 -68.4,78.2 -68.4,78.25 -68.4,78.3 -68.4,78.35 -68.4,78.4 -68.4,78.45 -68.4,78.5 -68.4,78.5 -68.419,78.5 -68.438,78.5 -68.457,78.5 -68.476,78.5 -68.495,78.5 -68.514,78.5 -68.533,78.5 -68.552,78.5 -68.571,78.5 -68.59,78.45 -68.59,78.4 -68.59,78.35 -68.59,78.3 -68.59,78.25 -68.59,78.2 -68.59,78.15 -68.59,78.1 -68.59,78.05 -68.59,78 -68.59,78 -68.571,78 -68.552,78 -68.533,78 -68.514,78 -68.495,78 -68.476,78 -68.457,78 -68.438,78 -68.419,78 -68.4))", "dataset_titles": "Flow cytometry enumeration of virus-like and bacteria-like abundance in Ace, Deep, \u0026 Organic lakes (Antarctica)", "datasets": [{"dataset_uid": "601626", "doi": "10.15784/601626", "keywords": "Ace Lake; Antarctica; Cryosphere; Deep Lake; Organic Lake; Vestfold Hills", "people": "Martinez-Martinez, Joaquin; Twining, Benjamin", "repository": "USAP-DC", "science_program": null, "title": "Flow cytometry enumeration of virus-like and bacteria-like abundance in Ace, Deep, \u0026 Organic lakes (Antarctica)", "url": "https://www.usap-dc.org/view/dataset/601626"}], "date_created": "Fri, 06 Aug 2021 00:00:00 GMT", "description": "Viruses are prevalent in aquatic environments where they reach up to five hundred million virus particles in a teaspoon of water. Ongoing discovery of viruses seems to confirm current understanding that all forms of life can host and be infected by viruses and that viruses are one of the largest reservoirs of unexplored genetic diversity on Earth. This study aims to better understand interactions between specific viruses and phytoplankton hosts and determine how these viruses may affect different algal groups present within lakes of the Vestfold Hills, Antarctica. These lakes (Ace, Organic and Deep)were originally derived from the ocean and contain a broad range of saline conditions with a similarly broad range of physicochemical characteristics resulting from isolation and low external influence for thousands of years. These natural laboratories allow examination of microbial processes and interactions that would be difficult to characterize elsewhere on earth. The project will generate extensive genomic information that will be made freely available. The project will also leverage the study of viruses and the genomic approaches employed to advance the training of undergraduate students and to engage and foster an understanding of Antarctic science and studies of microbes during a structured informal education program in Maine for the benefit of high school students.\r\n\r\nBy establishing the dynamics and interactions of (primarily) specific dsDNA virus groups in different habitats with different redox conditions throughout seasonal and inter annual cycles the project will learn about the biotic and abiotic factors that influence microbial community dynamics. This project does not require fieldwork in Antarctica. Instead, the investigators will leverage already collected and archived samples from three lakes that have concurrent measures of physicochemical information. Approximately 2 terabyte of Next Generation Sequencing (NGS) (including metagenomes, SSU rRNA amplicons and single virus genomes) will be generated from selected available samples through a Community Science Program (CSP) funded by the Joint Genome Institute. The investigators will employ bioinformatics to interrogate those sequence databases. In particular, they will focus on investigating the presence, phylogeny and co-occurrence of polintons, polinton-like viruses, virophages and large dsDNA phytoplankton viruses as well as of their putative eukaryotic microbial hosts. Bioinformatic analyses will be complemented with quantitative digital PCR and microbial association network analysis to detect specific virus-host interactions from co-occurrence spatial and temporal patterns. Multivariate analysis and network analyses will also be performed to investigate which abiotic factors most closely correlate with phytoplankton and virus abundances, temporal dynamics, and observed virus-phytoplankton associations within the three lakes. The results of this project will improve understanding of phytoplankton and their viruses as vital components of the carbon cycle in Antarctic, marine-derived aquatic environments, and likely in any other aquatic environment. Overall, this work will advance understanding of the genetic underpinnings of adaptations in unique Antarctic environments.", "east": 78.5, "geometry": "POINT(78.25 -68.495)", "instruments": null, "is_usap_dc": true, "keywords": "Vestfold Hills; AMD/US; LABORATORY; AMD; FIELD SURVEYS; USAP-DC; VIRUSES; USA/NSF", "locations": "Vestfold Hills", "north": -68.4, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Twining, Benjamin; Martinez-Martinez, Joaquin", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS; OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -68.59, "title": "Viral control of microbial communities in Antarctic lakes", "uid": "p0010237", "west": 78.0}, {"awards": "1947040 Postlethwait, John", "bounds_geometry": "POLYGON((-65.3 -63.3,-65 -63.3,-64.7 -63.3,-64.4 -63.3,-64.1 -63.3,-63.8 -63.3,-63.5 -63.3,-63.2 -63.3,-62.9 -63.3,-62.6 -63.3,-62.3 -63.3,-62.3 -63.47,-62.3 -63.64,-62.3 -63.81,-62.3 -63.98,-62.3 -64.15,-62.3 -64.32,-62.3 -64.49,-62.3 -64.66,-62.3 -64.83,-62.3 -65,-62.6 -65,-62.9 -65,-63.2 -65,-63.5 -65,-63.8 -65,-64.1 -65,-64.4 -65,-64.7 -65,-65 -65,-65.3 -65,-65.3 -64.83,-65.3 -64.66,-65.3 -64.49,-65.3 -64.32,-65.3 -64.15,-65.3 -63.98,-65.3 -63.81,-65.3 -63.64,-65.3 -63.47,-65.3 -63.3))", "dataset_titles": "18 SSU rDNA type sequences for Notoxcellia coronata (nov. sp.); 18 SSU rDNA type sequences for Notoxcellia picta (nov. sp.); Fish pictures and skin pathology of X-cell infection in Trematomus scotti.; Histopathology of X-cell xenomas in Trematomus scotti and Nototheniops larseni.; In situ hybridization of X-cell and host fish 18S SSU rRNA in alternate sections of tumor xenomas.; Metagenomic analysis of apparently healthy and tumor samples using Kaiju software ; microMRI analyses of Trematomus scotti Tsco_18_08 with X-cell xenomas; Morphological and pathological data of Trematomus scotti specimens captured on May 30th, 2018 in Andvord Bay.; Phylogenetic Analysis of Notoxcellia species.; Raw Illumina sequencing reads from skin tumors and visually healthy skins from Trematomus scotti and Nototheniops larseni; Temperature profiles at five fishing locations on the West Antarctic Peninsula during austral fall 2018.; Trematomus scotti with X-cell xenomas", "datasets": [{"dataset_uid": "601536", "doi": "10.15784/601536", "keywords": "Alveolata; Antarctica; Antarctic Peninsula; Cryosphere; Notoxcellia coronata; Notoxcellia picta; Perkinsozoa; Xcellidae", "people": "Postlethwait, John; Murray, Katrina N. ; Desvignes, Thomas; Kent, Michael L. ", "repository": "USAP-DC", "science_program": null, "title": "Histopathology of X-cell xenomas in Trematomus scotti and Nototheniops larseni.", "url": "https://www.usap-dc.org/view/dataset/601536"}, {"dataset_uid": "601496", "doi": "10.15784/601496", "keywords": "Andvord Bay; Antarctica; Cryosphere; Fish", "people": "Postlethwait, John; Lauridsen, Henrik; Desvignes, Thomas", "repository": "USAP-DC", "science_program": null, "title": "Fish pictures and skin pathology of X-cell infection in Trematomus scotti.", "url": "https://www.usap-dc.org/view/dataset/601496"}, {"dataset_uid": "200276", "doi": "", "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "18 SSU rDNA type sequences for Notoxcellia picta (nov. sp.)", "url": "https://www.ncbi.nlm.nih.gov/nuccore/OL630145"}, {"dataset_uid": "200275", "doi": "", "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "18 SSU rDNA type sequences for Notoxcellia coronata (nov. sp.)", "url": "https://www.ncbi.nlm.nih.gov/nuccore/OL630144"}, {"dataset_uid": "200262", "doi": "", "keywords": null, "people": null, "repository": "MorphoSource", "science_program": null, "title": "Trematomus scotti with X-cell xenomas", "url": "https://www.morphosource.org/projects/000405843?locale=en"}, {"dataset_uid": "601539", "doi": "10.15784/601539", "keywords": "Alveolata; Antarctica; Antarctic Peninsula; Cryosphere; Notoxcellia coronata; Notoxcellia picta; Perkinsozoa; Xcellidae", "people": "Desvignes, Thomas; Postlethwait, John", "repository": "USAP-DC", "science_program": null, "title": "In situ hybridization of X-cell and host fish 18S SSU rRNA in alternate sections of tumor xenomas.", "url": "https://www.usap-dc.org/view/dataset/601539"}, {"dataset_uid": "601538", "doi": "10.15784/601538", "keywords": "Alveolata; Antarctica; Antarctic Peninsula; Cryosphere; Notoxcellia coronata; Notoxcellia picta; Perkinsozoa; Xcellidae", "people": "Postlethwait, John; Desvignes, Thomas; Lauridsen, Henrik", "repository": "USAP-DC", "science_program": null, "title": "microMRI analyses of Trematomus scotti Tsco_18_08 with X-cell xenomas", "url": "https://www.usap-dc.org/view/dataset/601538"}, {"dataset_uid": "601537", "doi": "10.15784/601537", "keywords": "Alveolata; Antarctica; Antarctic Peninsula; Cryosphere; Notoxcellia coronata; Notoxcellia picta; Perkinsozoa; Xcellidae", "people": "Desvignes, Thomas; Postlethwait, John; Varsani, Arvind; Kraberger, Simona ; Fontenele, Rafaela S. ", "repository": "USAP-DC", "science_program": null, "title": "Metagenomic analysis of apparently healthy and tumor samples using Kaiju software ", "url": "https://www.usap-dc.org/view/dataset/601537"}, {"dataset_uid": "601501", "doi": "10.15784/601501", "keywords": "Alveolata; Antarctica; Antarctic Peninsula; Biology; Biosphere; Cryosphere; Notoxcellia coronata; Notoxcellia picta; Oceans; Perkinsozoa; Xcellidae", "people": "Postlethwait, John; Desvignes, Thomas; Varsani, Arvind", "repository": "USAP-DC", "science_program": null, "title": "Phylogenetic Analysis of Notoxcellia species.", "url": "https://www.usap-dc.org/view/dataset/601501"}, {"dataset_uid": "200277", "doi": "", "keywords": null, "people": null, "repository": "NCBI SRA", "science_program": null, "title": "Raw Illumina sequencing reads from skin tumors and visually healthy skins from Trematomus scotti and Nototheniops larseni", "url": "https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA789574"}, {"dataset_uid": "601495", "doi": "10.15784/601495", "keywords": "Antarctica; Antarctic Peninsula; Cryosphere", "people": "Desvignes, Thomas", "repository": "USAP-DC", "science_program": null, "title": "Temperature profiles at five fishing locations on the West Antarctic Peninsula during austral fall 2018.", "url": "https://www.usap-dc.org/view/dataset/601495"}, {"dataset_uid": "601494", "doi": "10.15784/601494", "keywords": "Andvord Bay; Antarctica; Cryosphere; Fish", "people": "Lauridsen, Henrik; Le Francois, Nathalie; Desvignes, Thomas; Postlethwait, John", "repository": "USAP-DC", "science_program": null, "title": "Morphological and pathological data of Trematomus scotti specimens captured on May 30th, 2018 in Andvord Bay.", "url": "https://www.usap-dc.org/view/dataset/601494"}], "date_created": "Thu, 01 Jul 2021 00:00:00 GMT", "description": "Overview:\r\nAntarctic biota face increasing stressors from warming oceans. A key question is: What will be the effect of warming waters on Antarctic biota? A gap in our knowledge is the identify of early harbingers of new stressors. In our recent field season, we unexpectedly discovered pink, wart-like neoplasms in Antarctic notothenioid fish, including Trematomus scotti (crowned notothen) and Nototheniops larseni (painted notothen). Neoplasms affected about 30% of T. scotti collected in Andvord Bay on the West Antarctic Peninsula and covered 10 to 30% of the bodies of affected individuals, usually in one contiguous patch. We collected samples from affected and apparently unaffected controls. We could not find evidence of any similar outbreak. Our overall goal is to learn the biological origins of this neoplasm and how it affects cellular function and organismal physiology. \r\nIntellectual Merit:\r\nAim 1: Pathogenic agents. Aim 1a: To test the hypothesis that a virus causes the neoplasm. Methods involve isolating and sequencing viral nucleic acids from neoplasms and from unaffected skin and comparing sequences to known viruses. Aim 1b: To test the hypothesis that neoplasms are hosts to parasites not present in healthy skin. Methods include tissue sections and DNA sequencing to find evidence of parasitic organisms. Significance: achieving Aim 1 will narrow down possible etiological agents. An untested possibility is that environmental contaminants cause the condition; exploring that hypothesis would require further sampling outside the limits of an EAGER proposal.\r\nAim 2: Cell-level pathology. Aim 2a: To test the hypothesis that the histopathology of the neoplasms is similar to other known skin neoplasias; alternatively, it might be a previously unknown type of neoplasia. Methods involve the examination of histological sections to identify pathology-specific characters. Aim 2b: To find effects of neoplasms on cell function. Methods involve performing whole-genome transcriptomics of affected and normal skin by RNA-seq and aligning reads to a T. scotti reference genome. Significance: achieving Aim 2 will define the cell biology and gene-expression phenotypes of the neoplasia, thus suggesting mechanisms that cause it.\r\n[Note: NSF deleted funds specifically to achieve the Aim 3, which nevertheless appears here to represent the original proposal.] Aim 3: Organismal pathology. Aim 3a: To test the hypothesis that the neoplasm has adverse effects on growth and physiology. Methods are to perform morphometrics in fish with neoplasms compared to age-matched controls from otolith studies. Aim 3b: To test the hypothesis that the neoplasia affects reproductive traits. Methods compare reproductive effort in affected and unaffected individuals. Significance: if the neoplasia has little consequences on growth and reproduction, our worry about its spread will be lessened, but if it is harmful, then Antarctic ecology, which largely depends on notothenioid fish, might be in danger.\r\nAchieving Aims 1-3 will advance knowledge by identifying the causes of a neoplasia outbreak in Antarctic fish. Work is potentially transformative because it might represent an early sign of Antarctic fish responses to the stress of global climate change. Proposed work would be the first to investigate a neoplasia outbreak in Antarctic fish. We will assess the project\u2019s success by whether we identify a causative agent and its effects on physiology.\r\nBroader Impacts:\r\nAim 4: Publicizing the neoplasia. We aim to raise awareness of the outbreak and publicize its distinct diagnostic features, including assays to detect it, by contributing to groups that track Antarctic ecosystems. \r\nAim 5: Inclusion. We will involve underrepresented groups in scientific research with authentic research experiences.\r\nAchieving Aims 4 and 5 will benefit society because they will disseminate to scientific and lay communities a potential early-warning system for the effects of an apparently new neoplasia affecting, at least locally, a large proportion of a fish population. Dissemination will stir research to determine whether this neoplasia outbreak is an isolated event or is becoming a general phenomenon, and thus a concern for Antarctic ecosystems. Proposed research will enhance research infrastructure by providing tools to identify the neoplasia. Finally, the project will broaden access to research careers by exposing underserved high school students and undergraduates to an exciting live research project.\r\n", "east": -62.3, "geometry": "POINT(-63.8 -64.15)", "instruments": null, "is_usap_dc": true, "keywords": "FIELD SURVEYS; PROTISTS; BENTHIC; FISH; Dallmann Bay; USAP-DC; Andvord Bay; NSF/USA; AMD/US; AMD", "locations": "Andvord Bay; Dallmann Bay", "north": -63.3, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Postlethwait, John; Varsani, Arvind; Desvignes, Thomas", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS", "repo": "USAP-DC", "repositories": "GenBank; Other; USAP-DC", "science_programs": null, "south": -65.0, "title": "EAGER: Origin and Physiological Consequences of a Neoplasm Outbreak in Antarctic Fish ", "uid": "p0010221", "west": -65.3}, {"awards": "1341736 Adams, Byron", "bounds_geometry": null, "dataset_titles": "Dataset DS-TAMS: Genetic diversity of Collembola from the Transantarctic Mountains; GenBank accession numbers MN619477 to MN619610; Meteoric 10Be data of soils from the Shackleton Glacier region; Shackleton Glacier region soil water-soluble geochemical data; Shackleton Glacier region water-soluble salt isotopes; Soil invertebrate surveys from the Shackleton Glacier region of Antarctica during the 2017-2018 austral summer", "datasets": [{"dataset_uid": "200258", "doi": "doi:10.6073/pasta/7959821e5f6f8d56d94bb6a26873b3ae", "keywords": null, "people": null, "repository": "Environmental Data Initiative", "science_program": null, "title": "Soil invertebrate surveys from the Shackleton Glacier region of Antarctica during the 2017-2018 austral summer", "url": "https://doi.org/10.6073/pasta/7959821e5f6f8d56d94bb6a26873b3ae"}, {"dataset_uid": "200174", "doi": "10.5883/DS-TAMS", "keywords": null, "people": null, "repository": "Barcode of Life Datasystems (BOLD)", "science_program": null, "title": "Dataset DS-TAMS: Genetic diversity of Collembola from the Transantarctic Mountains", "url": "http://dx.doi.org/10.5883/DS-TAMS"}, {"dataset_uid": "601419", "doi": "10.15784/601419", "keywords": "Antarctica; Cryosphere; Geochemistry; Nitrate; Shackleton Glacier; Stable Isotopes; Sulfate; Transantarctic Mountains", "people": "Diaz, Melisa A.; Gardner, Christopher B.; Lyons, W. Berry", "repository": "USAP-DC", "science_program": null, "title": "Shackleton Glacier region water-soluble salt isotopes", "url": "https://www.usap-dc.org/view/dataset/601419"}, {"dataset_uid": "200175", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "GenBank accession numbers MN619477 to MN619610", "url": "https://www.ncbi.nlm.nih.gov/nuccore/MN619477"}, {"dataset_uid": "601418", "doi": "10.15784/601418", "keywords": "Antarctica; Cryosphere; Geochemistry; Shackleton Glacier", "people": "Gardner, Christopher B.; Diaz, Melisa A.; Lyons, W. Berry", "repository": "USAP-DC", "science_program": null, "title": "Shackleton Glacier region soil water-soluble geochemical data", "url": "https://www.usap-dc.org/view/dataset/601418"}, {"dataset_uid": "601421", "doi": "10.15784/601421", "keywords": "Antarctica; Be-10; Beryllium-10; Cosmogenic Radionuclides; Cryosphere; Geochemistry; Geomorphology; Shackleton Glacier; SURFACE EXPOSURE DATES", "people": "Diaz, Melisa A.", "repository": "USAP-DC", "science_program": null, "title": "Meteoric 10Be data of soils from the Shackleton Glacier region", "url": "https://www.usap-dc.org/view/dataset/601421"}], "date_created": "Mon, 02 Nov 2020 00:00:00 GMT", "description": "The project will characterize the functional, taxonomic, biotic and abiotic drivers of soil ecosystems in the Trans Antarctic Mountains (one of the most remote and harsh terrestrial landscapes on the planet). The work will utilize new high-throughput DNA and RNA sequencing technologies to identify members of the microbial communities and determine if the microbial community structures are independent of local environmental heterogeneities. In addition the project will determine if microbial diversity and function are correlated with time since the last glacial maximum (LGM). The expected results will greatly contribute to our knowledge regarding rates of microbial succession and help define the some of the limits to life and life-maintaining processes on Earth.\u003cbr/\u003e\u003cbr/\u003eThe project will analyze genomes and RNA derived from these genomes to describe the relationships between biodiversity and ecosystem functioning from soils above and below LGM elevations and to correlate these with the environmental drivers associated with their development during the last ~18,000 years. The team will identify the taxonomic diversity and the functional genetic composition within a broad suite of soil biota and examine their patterns of assembly and distribution within the framework of their geological legacies. The project will mentor participants from undergraduate students to postdoctoral researchers and prepare them to effectively engage in research to meet their career aspirations. The project will contribute to ongoing public education efforts through relationships with K-12 teachers and administrators- to include University-Public School partnerships. Less formal activities include public lecture series and weblogs aimed at providing information on Antarctic polar desert ecosystems to the general public. Targeted classrooms near each PI\u0027s institution will participate in online, real-time discussions about current topics in Antarctic ecosystems research.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "FIELD INVESTIGATION; AMD/US; LABORATORY; AMD; USA/NSF; TERRESTRIAL ECOSYSTEMS; Transantarctic Mountains; USAP-DC", "locations": "Transantarctic Mountains", "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Earth Sciences", "paleo_time": null, "persons": "Adams, Byron; Fierer, Noah; Wall, Diana; Diaz, Melisa A.; Gardner, Christopher B.; Lyons, W. Berry", "platforms": "OTHER \u003e PHYSICAL MODELS \u003e LABORATORY; LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION", "repo": "Environmental Data Initiative", "repositories": "Other; USAP-DC", "science_programs": null, "south": null, "title": "Collaborative Research: The Role of Glacial History on the Structure and Functioning of Ecological Communities in the Shackleton Glacier Region of the Transantarctic Mountains", "uid": "p0010140", "west": null}, {"awards": "1620976 Johnson, Sarah", "bounds_geometry": "POLYGON((160 -77,160.3 -77,160.6 -77,160.9 -77,161.2 -77,161.5 -77,161.8 -77,162.1 -77,162.4 -77,162.7 -77,163 -77,163 -77.1,163 -77.2,163 -77.3,163 -77.4,163 -77.5,163 -77.6,163 -77.7,163 -77.8,163 -77.9,163 -78,162.7 -78,162.4 -78,162.1 -78,161.8 -78,161.5 -78,161.2 -78,160.9 -78,160.6 -78,160.3 -78,160 -78,160 -77.9,160 -77.8,160 -77.7,160 -77.6,160 -77.5,160 -77.4,160 -77.3,160 -77.2,160 -77.1,160 -77))", "dataset_titles": "GenBank Sequence Read Archive with accession numbers SRR8217969 - SRR8217976 and project accession PRJNA506221", "datasets": [{"dataset_uid": "200164", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "GenBank Sequence Read Archive with accession numbers SRR8217969 - SRR8217976 and project accession PRJNA506221", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA506221/"}], "date_created": "Tue, 01 Sep 2020 00:00:00 GMT", "description": "Despite recent advances, we still know little about how life and its traces persist in extremely harsh conditions. What survival strategies do cells employ when pushed to their limit? Using a new technique, this project will investigate whether Antarctic paleolakes harbor \"microbial seed banks,\" or caches of viable microbes adapted to past paleoenvironments that could help transform our understanding of how cells survive over ancient timescales. Findings from this investigation could also illuminate novel DNA repair pathways with possible biomedical and biotechnology applications and help to refine life detection strategies for Mars. The project will bring Antarctic research to Georgetown University\u0027\u0027s campus for the first time, providing training opportunities in cutting edge analytical techniques for multiple students and a postdoctoral fellow. The field site will be the McMurdo Dry Valleys, which provide an unrivaled opportunity to investigate fundamental questions about the persistence of microbial life. Multiple lines of evidence, from interbedded and overlying ashfall deposits to parameterized models, suggest that the large-scale landforms there have remained essentially fixed as far back as the middle of the Miocene Epoch (i.e., ~8 million years ago). This geologic stability, coupled with geographic isolation and a steady polar climate, mean that biological activity has probably undergone few qualitative changes over the last one to two million years. The team will sample paleolake facies using sterile techniques from multiple Dry Valleys sites and extract DNA from entombed organic material. Genetic material will then be sequenced using Pacific Biosciences\u0027\u0027 Single Molecule, Real-Time DNA sequencing technology, which sequences native DNA as opposed to amplified DNA, thereby eliminating PCR primer bias, and enables read lengths that have never before been possible. The data will be analyzed with a range of bioinformatic techniques, with results that stand to impact our understanding of cell biology, Antarctic paleobiology, microbiology and biogeography, biotechnology, and planetary science.", "east": 163.0, "geometry": "POINT(161.5 -77.5)", "instruments": null, "is_usap_dc": true, "keywords": "BACTERIA/ARCHAEA; CYANOBACTERIA (BLUE-GREEN ALGAE); LABORATORY; Dry Valleys; ECOSYSTEM FUNCTIONS; COMMUNITY DYNAMICS", "locations": "Dry Valleys", "north": -77.0, "nsf_funding_programs": "Antarctic Earth Sciences; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Johnson, Sarah", "platforms": "OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repo": "NCBI GenBank", "repositories": "Other", "science_programs": null, "south": -78.0, "title": "EAGER: Single-Molecule DNA Sequencing of Antarctic Paleolakes", "uid": "p0010125", "west": 160.0}, {"awards": "1341661 Near, Thomas", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "Phylogenomics of Antarctic notothenioid fishes", "datasets": [{"dataset_uid": "601262", "doi": "10.15784/601262", "keywords": "Adaptive Radiation; Antarctica; Cryosphere; Fish; Notothenioidei; Phylogeny; Southern Ocean; Speciation", "people": "Dornburg, Alex; Near, Thomas", "repository": "USAP-DC", "science_program": null, "title": "Phylogenomics of Antarctic notothenioid fishes", "url": "https://www.usap-dc.org/view/dataset/601262"}, {"dataset_uid": "601264", "doi": null, "keywords": "Adaptive Radiation; Antarctica; Cryosphere; Fish; Notothenioidei; Phylogeny; Southern Ocean; Speciation", "people": "Dornburg, Alex; Near, Thomas", "repository": "USAP-DC", "science_program": null, "title": "Phylogenomics of Antarctic notothenioid fishes", "url": "https://www.usap-dc.org/view/dataset/601264"}], "date_created": "Sat, 29 Feb 2020 00:00:00 GMT", "description": "Understanding how groups of organisms respond to climate change is fundamentally important to assessing the impacts of human activities as well as understanding how past climatic shifts have shaped biological diversity over deep stretches of time. The fishes occupying the near-shore marine habitats around Antarctica are dominated by one group of closely related species called notothenioids. It appears dramatic changes in Antarctic climate were important in the origin and evolutionary diversification of this economically important lineage of fishes. Deposits of fossil fishes in Antarctica that were formed when the continent was experiencing milder temperatures show that the area was home to a much more diverse array of fish lineages. Today the waters of the Southern Ocean are very cold, and often below freezing, but notothenioids fishes exhibit a number of adaptions to live in this harsh set of marine habitats, including the presence of anti-freeze proteins. This research project will collect DNA sequences from hundreds of genes to infer the genealogical relationships of nearly all 124 notothenioid species, and use mathematical techniques to estimate the ages of species and lineages. Knowledge on the timing of evolutionary divergence in notothenioids will allow investigators to assess if timing of previous major climatic shifts in Antarctica are correlated with key events in the formation of the modern Southern Ocean fish fauna. The project will also further the NSF goals of making scientific discoveries available to the general public and of training new generations of scientists. The project will support educational outreach activities to teenager groups and to the general public through a natural history museum exhibit and other public lectures. It will provide professional training opportunities for graduate students and a postdoctoral research scholar. \u003cbr/\u003e\u003cbr/\u003eAdaptive radiation, where lineages experience high rates of evolutionary diversification coincident with ecological divergence, is mostly studied in island ecosystems. Notothenioids dominate the fish fauna of the Southern Ocean and exhibit antifreeze glycoproteins that allow occupation of the subzero waters. Notothenioids are noted as one of the only examples of adaptive radiation among marine fishes, but the evolutionary history of diversification and radiation into different ecological habitats is poorly understood. This research will generate a species phylogeny (evolutionary history) for nearly all of the 124 recognized notothenioid species to investigate the mechanisms of adaptive radiation in this lineage. The phylogeny is inferred from approximately 350 genes sampled using next generation DNA sequencing and related techniques. Morphometric data are taken for museum specimens to investigate the tempo of morphological diversification and to determine if there are correlations between rates of lineage diversification and the origin of morphological disparity. The patterns of lineage, morphological, and ecological diversification in the notothenioid radiation will be compared to the paleoclimatic record to determine if past instances of global climate change have shaped the evolutionary diversification of this lineage of polar-adapted fishes.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "Southern Ocean; FISH; NOT APPLICABLE; USAP-DC; Fish; AMD; MARINE ECOSYSTEMS; Notothenioidei; AMD/US; Phylogeny; USA/NSF", "locations": "Southern Ocean", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Near, Thomas", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -90.0, "title": "Phylogenomic Study of Adaptive Radiation in Antarctic Fishes", "uid": "p0010087", "west": -180.0}, {"awards": "1443578 Schmidt, Steven", "bounds_geometry": "POLYGON((161.5 -77.5,161.7 -77.5,161.9 -77.5,162.1 -77.5,162.3 -77.5,162.5 -77.5,162.7 -77.5,162.9 -77.5,163.1 -77.5,163.3 -77.5,163.5 -77.5,163.5 -77.53,163.5 -77.56,163.5 -77.59,163.5 -77.62,163.5 -77.65,163.5 -77.68,163.5 -77.71,163.5 -77.74,163.5 -77.77,163.5 -77.8,163.3 -77.8,163.1 -77.8,162.9 -77.8,162.7 -77.8,162.5 -77.8,162.3 -77.8,162.1 -77.8,161.9 -77.8,161.7 -77.8,161.5 -77.8,161.5 -77.77,161.5 -77.74,161.5 -77.71,161.5 -77.68,161.5 -77.65,161.5 -77.62,161.5 -77.59,161.5 -77.56,161.5 -77.53,161.5 -77.5))", "dataset_titles": "16S and 18S amplicon sequencing of Antarctic cryoconite holes; Genomes of Antarctic ssDNA viruses (GenBank accession numbers MN311489-MN311492 and MN328267-MN328291); Metadata from samples (in the process of submitting to EDI; will update with DOI once completed); Microbial species-area relationships in Antarctic cryoconite holes; Soil microbial communities of a mountain landscape, McMurdo Dry Valleys, Antarctica", "datasets": [{"dataset_uid": "200281", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Microbial species-area relationships in Antarctic cryoconite holes", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA668398/"}, {"dataset_uid": "200279", "doi": "", "keywords": null, "people": null, "repository": "GitHub", "science_program": null, "title": "Metadata from samples (in the process of submitting to EDI; will update with DOI once completed)", "url": "https://github.com/pacificasommers/Cryoconite-metadata"}, {"dataset_uid": "200280", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Soil microbial communities of a mountain landscape, McMurdo Dry Valleys, Antarctica", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA721735/"}, {"dataset_uid": "200084", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Genomes of Antarctic ssDNA viruses (GenBank accession numbers MN311489-MN311492 and MN328267-MN328291)", "url": ""}, {"dataset_uid": "200081", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "16S and 18S amplicon sequencing of Antarctic cryoconite holes", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA480849/"}], "date_created": "Fri, 01 Nov 2019 00:00:00 GMT", "description": "Cryoconite holes are pockets of life completely encased in otherwise barren glacial ice. These pockets of life form when dust blown onto the ice melts a small, largely isolated hole that can function as its own tiny ecosystem. This dust can contain microorganisms such as bacteria, algae, or microscopic animals. The microorganisms within the hole interact and carry out functions typical of a larger ecosystem, such as a forest. Cryoconite holes are especially important in extreme cold environments such as the Antarctic Dry Valleys, where they function as repositories of life. Because cryoconite holes are mostly enclosed and persist for years, they can be tracked over time to test fundamental scientific questions about how communities of interacting organisms develop to become fully functioning ecosystems. This project will sample existing and experimentally created cryoconite holes to understand how these ecosystems develop and to what degree random processes (such as which organisms get there first) affect the final community composition and functioning. The results will not only improve our understanding of how microbial communities assemble and affect the functioning of microecosystems such as cryoconite holes, but also how the processes of community assembly affect functioning of larger ecosystems, such as forests. A better understanding of community establishment, development, and response to abiotic factors are essential to forecasting ecological responses to environmental change.\u003cbr/\u003e\u003cbr/\u003eIt is essential to unravel the links between community assembly, biodiversity, and nutrient cycling across numerous ecosystems because these are critical factors determining ecological responses to environmental change. The unique, largely isolated nature of cryoconite holes provides an experimental system that will advance fundamental understanding of the processes (e.g., stochastic dynamics such as dispersal limitation, assembly order, and ecological drift) driving community assembly. This project will use a field sampling campaign and a number of manipulative experiments to test a hypothesis that unites theory in community and ecosystem ecology: the degree to which stochastic processes guide microbial community assembly and affects regional patterns in biodiversity and ecosystem processes. Cryoconite holes will be sampled to compare community composition, environmental factors, and ecosystem functioning between hydrologically connected and isolated holes. New cryoconite holes will also be constructed and monitored over the course of two growing seasons to specifically alter assembly order and community size, thereby pairing a unique manipulative experiment with field surveys to address questions with relevance to the Antarctic and beyond. Amplicon sequencing, metagenomics, microscopy, sensitive environmental chemistry methods, and photosynthesis and respiration measurements will be used to test a series of sub-hypotheses that relate stochasticity to patterns in regional biodiversity, heterogeneity in environmental factors, and ecosystem processes.", "east": 163.5, "geometry": "POINT(162.5 -77.65)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "GLACIERS; USAP-DC; FIELD INVESTIGATION; Antarctica", "locations": "Antarctica", "north": -77.5, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Schmidt, Steven; Cawley, Kaelin; Fountain, Andrew", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION", "repo": "NCBI GenBank", "repositories": "Other", "science_programs": null, "south": -77.8, "title": "Collaborative Research: Stochasticity and Cryoconite Community Assembly and Function", "uid": "p0010063", "west": 161.5}, {"awards": "1543412 Reinfelder, John", "bounds_geometry": null, "dataset_titles": "16S rRNA gene libraries of krill gut microbial communities; Microbial gene libraries of krill gut microbial communities", "datasets": [{"dataset_uid": "601171", "doi": "10.15784/601171", "keywords": "Antarctica; Biology; Biosphere; Cryosphere; Krill; LTER Palmer Station; Microbiome; Oceans; Southern Ocean", "people": "Reinfelder, John", "repository": "USAP-DC", "science_program": "LTER", "title": "16S rRNA gene libraries of krill gut microbial communities", "url": "https://www.usap-dc.org/view/dataset/601171"}, {"dataset_uid": "200024", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Microbial gene libraries of krill gut microbial communities", "url": "https://nam02.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fbioproject%2F531145\u0026amp;data=02%7C01%7Creinfeld%40envsci.rutgers.edu%7C7e30a0192dc748ab271408d6b9d57d08%7Cb92d2b234d35447093ff69aca6632ffe%7C1%7C0%7C636900723909188941\u0026amp;sdata=G6cNg4bBHzeikrWSCYITcT6XS3NLWwjQ1yNdwtrALPc%3D\u0026amp;reserved=0"}], "date_created": "Sun, 31 Mar 2019 00:00:00 GMT", "description": "The goal of this project was to conduct a preliminary assessment of gut microbiomes in Antarctic krill (Euphasia superba) collected in coastal waters west of the Antarctic Peninsula and identify organisms potentially capable of catalyzing the production of methylmercury. DNA was extracted from composite krill digestive tracts and eukaryotic DNA removed. Prokaryotic microbial DNA extracted from krill digestive tracts was sequenced and gene libraries were constructed. Genera of anaerobic microorganisms which are known to support mercury methylation were identified.", "east": -68.2816, "geometry": "POINT(-69.09295 -66.8017)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "BACTERIA/ARCHAEA; USAP-DC; Antarctica; NOT APPLICABLE", "locations": "Antarctica", "north": -65.8708, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Schaefer, Jeffra; Reinfelder, John; Barkar, T.", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "USAP-DC", "repositories": "Other; USAP-DC", "science_programs": "LTER", "south": -67.7326, "title": "Methylmercury in Antarctic Krill Microbiomes", "uid": "p0010023", "west": -69.9043}, {"awards": "1341612 Bowser, Samuel", "bounds_geometry": null, "dataset_titles": "Aerial survey of Explorers Cove shoreline, late January 2005; Astrammina rara genome sequencing and assembly; Astrammina triangularis genome sequencing and assembly; Crithionina delacai mitochondrial genome sequence and assembly; Scanning electron micrographs: Influence of heavy metal (Pb, Cd) exposure on shell morphogenesis in Astrammina rara, a giant agglutinated Antarctic foraminiferan protist", "datasets": [{"dataset_uid": "200090", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Astrammina rara genome sequencing and assembly", "url": "https://dataview.ncbi.nlm.nih.gov/object/PRJNA521081?reviewer=25e190ih1svottjkrrpfa7huoe"}, {"dataset_uid": "200089", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Astrammina triangularis genome sequencing and assembly", "url": "https://dataview.ncbi.nlm.nih.gov/object/PRJNA521279?reviewer=g418tpq02sif2g6do94dpmmjdv"}, {"dataset_uid": "601138", "doi": "10.15784/601138", "keywords": "Antarctica; Biology; Biosphere; Cryosphere; Foraminifera; Heavy Metal Toxicity; Scanning Electron Microscop; Scanning Electron Microscope (SEM) images; scanning electron microscopy; Transantarctic Mountains", "people": "Andreas, Amanda; Bowser, Samuel", "repository": "USAP-DC", "science_program": null, "title": "Scanning electron micrographs: Influence of heavy metal (Pb, Cd) exposure on shell morphogenesis in Astrammina rara, a giant agglutinated Antarctic foraminiferan protist", "url": "https://www.usap-dc.org/view/dataset/601138"}, {"dataset_uid": "200091", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Crithionina delacai mitochondrial genome sequence and assembly", "url": "https://dataview.ncbi.nlm.nih.gov/object/PRJNA592714?reviewer=ivse8455h3gfaiilg4nqle0vm1"}, {"dataset_uid": "601229", "doi": "10.15784/601229", "keywords": "Aerial Imagery; Antarctica; Cryosphere; Delta; Digital Single Lens Reflex (DSLR) Camera; Freshwater Input; Helicopter; Moat; Shoreline Survey; Small Ponds; Snow Melt; Tide Pools", "people": "Bowser, Samuel; Alexander, Steve", "repository": "USAP-DC", "science_program": null, "title": "Aerial survey of Explorers Cove shoreline, late January 2005", "url": "https://www.usap-dc.org/view/dataset/601229"}], "date_created": "Thu, 29 Nov 2018 00:00:00 GMT", "description": "Agglutinated foraminifera (forams for short) are early-evolving, single-celled organisms. These \"living fossils\" construct protective shells using sediment grains held together by adhesive substances that they secrete. During shell construction, agglutinated forams display amazing properties of selection - for example, some species build their shells of clear quartz grains, while other species use only grains of a specific size. Understanding how these single cells assemble complex structures may contribute to nanotechnology by enabling people to use forams as \"cellular machines\" to aid in the construction of nano-devices. This project will analyze the genomes of at least six key foram species, and then \"mine\" these genomes for technologically useful products and processes. The project will focus initially on the adhesive materials forams secrete, which may have wide application in biomedicine and biotechnology. Furthermore, the work will further develop a molecular toolkit which could open up new avenues of research on the physiology, ecology, and population dynamics of this important group of Antarctic organisms. The project will also further the NSF goals of making scientific discoveries available to the general public and of training new generations of scientists. Educational experiences related to the \"thrill of scientific exploration and discovery\" for students and the general public will be provided through freely-available short films and a traveling art/science exhibition. The project will also provide hands-on research opportunities for undergraduate students.\u003cbr/\u003e\u003cbr/\u003eExplorers Cove, situated on the western shore of McMurdo Sound, harbors a unique population of foraminiferan taxa at depths accessible by scuba diving that otherwise are primarily found in the deep sea. The project will use next-generation DNA sequencing and microdissection methods to obtain and analyze nuclear and mitochondrial genomes from crown members of two species each from three distinct, early-evolving foraminiferal groups. It will also use next generation sequencing methods to characterize the in-situ prokaryotic assemblages (microbiomes) of one of these groups and compare them to reference sediment microbiomes. The phyogenomic studies of the targeted Antarctic genera will help fill significant gaps in our current understanding of early foram evolution. Furthermore, comparative genomic analyses of these six species are expected to yield a better understanding of the physiology of single-chambered agglutinated forams, especially the bioadhesive proteins and regulatory factors involved in shell composition and morphogenesis. Additionally, the molecular basis of cold adaptation in forams will be examined, particularly with respect to key proteins.", "east": null, "geometry": null, "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "USAP-DC; NOT APPLICABLE", "locations": null, "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Bowser, Samuel", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "NCBI GenBank", "repositories": "Other; USAP-DC", "science_programs": null, "south": null, "title": "Assembling and Mining the Genomes of Giant Antarctic Foraminifera", "uid": "p0000004", "west": null}, {"awards": "1246292 Cary, Stephen", "bounds_geometry": "POLYGON((161.36062 -77.20215,161.610171 -77.20215,161.859722 -77.20215,162.109273 -77.20215,162.358824 -77.20215,162.608375 -77.20215,162.857926 -77.20215,163.107477 -77.20215,163.357028 -77.20215,163.606579 -77.20215,163.85613 -77.20215,163.85613 -77.291278,163.85613 -77.380406,163.85613 -77.469534,163.85613 -77.558662,163.85613 -77.64779,163.85613 -77.736918,163.85613 -77.826046,163.85613 -77.915174,163.85613 -78.004302,163.85613 -78.09343,163.606579 -78.09343,163.357028 -78.09343,163.107477 -78.09343,162.857926 -78.09343,162.608375 -78.09343,162.358824 -78.09343,162.109273 -78.09343,161.859722 -78.09343,161.610171 -78.09343,161.36062 -78.09343,161.36062 -78.004302,161.36062 -77.915174,161.36062 -77.826046,161.36062 -77.736918,161.36062 -77.64779,161.36062 -77.558662,161.36062 -77.469534,161.36062 -77.380406,161.36062 -77.291278,161.36062 -77.20215))", "dataset_titles": "Carbon-fixation rates and associated microbial communities; Carbon-fixation rates and associated microbial communities residing in arid and ephemerally wet Antarctic Dry Valley soils; Importance of Heterotrophic and Phototrophic N2 Fixation in the McMurdo Dry Valleys ; Microbial community composition of transiently wetted Antarctic Dry Valley soils.; Microbial population dynamics along a terrestrial Antarctic moisture gradient; Microbial population dynamics along a terrestrial wetted gradient", "datasets": [{"dataset_uid": "002736", "doi": "", "keywords": null, "people": null, "repository": "EMBL", "science_program": null, "title": "Microbial population dynamics along a terrestrial Antarctic moisture gradient", "url": "https://www.ebi.ac.uk/ena/data/view/PRJEB27415"}, {"dataset_uid": "200013", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Importance of Heterotrophic and Phototrophic N2 Fixation in the McMurdo Dry Valleys ", "url": "https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA505820"}, {"dataset_uid": "200015", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Microbial community composition of transiently wetted Antarctic Dry Valley soils.", "url": "https://www.ncbi.nlm.nih.gov/popset/?term=KP836071%20to%20KP836108"}, {"dataset_uid": "200014", "doi": "", "keywords": null, "people": null, "repository": "EMBL", "science_program": null, "title": "Microbial population dynamics along a terrestrial wetted gradient", "url": "https://www.ebi.ac.uk/ena/data/view/PRJEB7939"}, {"dataset_uid": "002737", "doi": "", "keywords": null, "people": null, "repository": "KNB", "science_program": null, "title": "Carbon-fixation rates and associated microbial communities residing in arid and ephemerally wet Antarctic Dry Valley soils", "url": "https://knb.ecoinformatics.org/view/knb.756.1"}, {"dataset_uid": "002738", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Carbon-fixation rates and associated microbial communities", "url": "https://www.ncbi.nlm.nih.gov/protein/?term=craig%20cary"}], "date_created": "Wed, 14 Mar 2018 00:00:00 GMT", "description": "The McMurdo Dry Valleys in Antarctica are recognized as being the driest, coldest and probably one of the harshest environments on Earth. In addition to the lack of water, the biota in the valleys face a very limited supply of nutrients such as nitrogen compounds - necessary for protein synthesis. The glacial streams of the Dry Valleys have extensive cyanobacterial (blue green algae) mats that are a major source of carbon and nitrogen compounds to biota in this region. While cyanobacteria in streams are important as a source of these compounds, other non-photosynthetic bacteria also contribute a significant fraction (~50%) of fixed nitrogen compounds to valley biota. This research effort will involve an examination of exactly which non-phototrophic bacteria are involved in nitrogen fixation and what environmental factors are responsible for controlling nitrogen fixation by these microbes. This work will resolve the environmental factors that control the activity, abundance and diversity of nitrogen-fixing microbes across four of the McMurdo Dry Valleys. This will allow for comparisons among sites of differing latitude, temperature, elevation and exposure to water. These results will be integrated into a landscape wetness model that will help determine the impact of both cyanobacterial and non-photosynthetic nitrogen fixing microorganisms in this very harsh environment.\u003cbr/\u003e\u003cbr/\u003eThe Dry Valleys in many ways resemble the Martian environment, and understanding the primitive life and very simple nutrient cycling in the Dry Valleys has relevance for understanding how life might have once existed on other planets. Furthermore, the study of microbes from extreme environments has resulted in numerous biotechnological applications such as the polymerase chain reaction for amplifying DNA and mechanisms for freeze resistance in agricultural crops. Thus, this research should yield insights into how biota survive in extreme environments, and these insights could lead to other commercial applications.", "east": 163.85613, "geometry": "POINT(162.608375 -77.64779)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "NOT APPLICABLE; Antarctica; USAP-DC; RIVERS/STREAM", "locations": "Antarctica", "north": -77.20215, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Cary, Stephen", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "EMBL", "repositories": "Other", "science_programs": null, "south": -78.09343, "title": "Collaborative Research: Importance of Heterotrophic and Phototrophic N2 Fixation in the McMurdo Dry Valleys on Local, Regional and Landscape Scales", "uid": "p0000235", "west": 161.36062}, {"awards": "1142108 Koch, Paul", "bounds_geometry": "POLYGON((-180 -55.1,-168.1 -55.1,-156.2 -55.1,-144.3 -55.1,-132.4 -55.1,-120.5 -55.1,-108.6 -55.1,-96.7 -55.1,-84.8 -55.1,-72.9 -55.1,-61 -55.1,-61 -57.4,-61 -59.7,-61 -62,-61 -64.3,-61 -66.6,-61 -68.9,-61 -71.2,-61 -73.5,-61 -75.8,-61 -78.1,-72.9 -78.1,-84.8 -78.1,-96.7 -78.1,-108.6 -78.1,-120.5 -78.1,-132.4 -78.1,-144.3 -78.1,-156.2 -78.1,-168.1 -78.1,180 -78.1,178.47 -78.1,176.94 -78.1,175.41 -78.1,173.88 -78.1,172.35 -78.1,170.82 -78.1,169.29 -78.1,167.76 -78.1,166.23 -78.1,164.7 -78.1,164.7 -75.8,164.7 -73.5,164.7 -71.2,164.7 -68.9,164.7 -66.6,164.7 -64.3,164.7 -62,164.7 -59.7,164.7 -57.4,164.7 -55.1,166.23 -55.1,167.76 -55.1,169.29 -55.1,170.82 -55.1,172.35 -55.1,173.88 -55.1,175.41 -55.1,176.94 -55.1,178.47 -55.1,-180 -55.1))", "dataset_titles": "Southern Ocean Pinnipeds", "datasets": [{"dataset_uid": "000242", "doi": "", "keywords": null, "people": null, "repository": "BCO-DMO", "science_program": null, "title": "Southern Ocean Pinnipeds", "url": "https://www.bco-dmo.org/project/726874"}], "date_created": "Wed, 28 Feb 2018 00:00:00 GMT", "description": "Building on previously funded NSF research, the use of paleobiological and paleogenetic data from mummified elephant seal carcasses found along the Dry Valleys and Victoria Land Coast in areas that today are too cold to support seal colonies (Mirougina leonina; southern elephant seals; SES) supports the former existence of these seals in this region. The occurrence and then subsequent disappearance of these SES colonies is consistent with major shifts in the Holocene climate to much colder conditions at the last ~1000 years BCE). \u003cbr/\u003e\u003cbr/\u003eFurther analysis of the preserved remains of three other abundant pinnipeds ? crabeater (Lobodon carciophagus), Weddell (Leptonychotes weddelli) and leopard (Hydrurga leptonyx) will be studied to track changes in their population size (revealed by DNA analysis) and their diet (studied via stable isotope analysis). Combined with known differences in life history, preferred ice habitat and ecosystem sensitivity among these species, this paleoclimate proxy data will be used to assess their exposure and sensitivity to climate change in the Ross Sea region during the past ~1-2,000 years", "east": -61.0, "geometry": "POINT(-128.15 -66.6)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "USAP-DC; NOT APPLICABLE", "locations": null, "north": -55.1, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Koch, Paul; Costa, Daniel; Hoelzel, A. Rus", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "BCO-DMO", "repositories": "BCO-DMO", "science_programs": null, "south": -78.1, "title": "Collaborative Research: Exploring the Vulnerability of Southern Ocean Pinnipeds to Climate Change - An Integrated Approach", "uid": "p0000410", "west": 164.7}, {"awards": "1141936 Foreman, Christine", "bounds_geometry": "POINT(112.085 -79.467)", "dataset_titles": "Molecular Level Characterization of Dissolved Organic Carbon and Microbial Diversity in the WAIS Divide Replicate Core", "datasets": [{"dataset_uid": "600133", "doi": "10.15784/600133", "keywords": "Antarctica; Biology; Biosphere; Cryosphere; Genetic Sequences; Glaciers/Ice Sheet; Glaciology; Ice Core Records; Paleoclimate; WAIS divide; WAIS Divide Ice Core", "people": "Foreman, Christine", "repository": "USAP-DC", "science_program": "WAIS Divide Ice Core", "title": "Molecular Level Characterization of Dissolved Organic Carbon and Microbial Diversity in the WAIS Divide Replicate Core", "url": "https://www.usap-dc.org/view/dataset/600133"}], "date_created": "Thu, 05 Nov 2015 00:00:00 GMT", "description": "This award supports a detailed, molecular level characterization of dissolved organic carbon and microbes in Antarctic ice cores. Using the most modern biological (genomic), geochemical techniques, and advanced chemical instrumentation researchers will 1) optimize protocols for collecting, extracting and amplifying DNA from deep ice cores suitable for use in next generation pyrosequencing; 2) determine the microbial diversity within the ice core; and 3) obtain and analyze detailed molecular characterizations of the carbon in the ice by ultrahigh resolution Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS). With this pilot study investigators will be able to quantify the amount of material (microbial biomass and carbon) required to perform these characterizations, which is needed to inform future ice coring projects. The ultimate goal will be to develop protocols that maximize the yield, while minimizing the amount of ice required. The broader impacts include education and outreach at both the local and national levels. As a faculty mentor with the American Indian Research Opportunities and BRIDGES programs at Montana State University, Foreman will serve as a mentor to a Native American student in the lab during the summer months. Susan Kelly is an Education and Outreach Coordinator with a MS degree in Geology and over 10 years of experience in science outreach. She will coordinate efforts for comprehensive educational collaboration with the Hardin School District on the Crow Indian Reservation in South-central Montana.", "east": 112.085, "geometry": "POINT(112.085 -79.467)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e CHEMICAL METERS/ANALYZERS \u003e ADS; IN SITU/LABORATORY INSTRUMENTS \u003e CORERS \u003e CORING DEVICES; IN SITU/LABORATORY INSTRUMENTS \u003e SPECTROMETERS/RADIOMETERS \u003e MASS SPECTROMETERS", "is_usap_dc": true, "keywords": "LABORATORY; Dissolved Organic Carbon; WAIS divide; FIELD SURVEYS; Microbes; Ice Core; Not provided; molecular; Antarctic; FIELD INVESTIGATION; Microbial Diversity; pyrosequencing; DNA", "locations": "Antarctic; WAIS divide", "north": -79.467, "nsf_funding_programs": "Antarctic Glaciology", "paleo_time": null, "persons": "Foreman, Christine", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS; OTHER \u003e PHYSICAL MODELS \u003e LABORATORY; LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION; Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": "WAIS Divide Ice Core", "south": -79.467, "title": "Molecular Level Characterization of Dissolved Organic Carbon and Microbial Diversity in the WAIS Divide Replicate Core", "uid": "p0000342", "west": 112.085}, {"awards": "0944201 Hofmann, Gretchen", "bounds_geometry": "POLYGON((-180 -68,-177 -68,-174 -68,-171 -68,-168 -68,-165 -68,-162 -68,-159 -68,-156 -68,-153 -68,-150 -68,-150 -69,-150 -70,-150 -71,-150 -72,-150 -73,-150 -74,-150 -75,-150 -76,-150 -77,-150 -78,-153 -78,-156 -78,-159 -78,-162 -78,-165 -78,-168 -78,-171 -78,-174 -78,-177 -78,180 -78,178 -78,176 -78,174 -78,172 -78,170 -78,168 -78,166 -78,164 -78,162 -78,160 -78,160 -77,160 -76,160 -75,160 -74,160 -73,160 -72,160 -71,160 -70,160 -69,160 -68,162 -68,164 -68,166 -68,168 -68,170 -68,172 -68,174 -68,176 -68,178 -68,-180 -68))", "dataset_titles": "Effect of Ocean Acidification on Early Life History Stages of the Antarctic Sea Urchins Sterechinus Neumayeri", "datasets": [{"dataset_uid": "600112", "doi": "10.15784/600112", "keywords": "Antarctica; Biosphere; Oceans; Southern Ocean", "people": "Hofmann, Gretchen", "repository": "USAP-DC", "science_program": null, "title": "Effect of Ocean Acidification on Early Life History Stages of the Antarctic Sea Urchins Sterechinus Neumayeri", "url": "https://www.usap-dc.org/view/dataset/600112"}], "date_created": "Tue, 23 Dec 2014 00:00:00 GMT", "description": "Abstract\u003cbr/\u003e\u003cbr/\u003eThe research examine the effects of ocean acidification on embryos and larvae of a contemporary calcifier in the coastal waters of Antarctica, the sea urchin Sterechinus neumayeri. The effect of future ocean acidification is projected to be particularly threatening to calcifying marine organisms in coldwater, high latitude seas, making tolerance data on these organisms a critical research need in Antarctic marine ecosystems. Due to a high magnesium (Mg) content of their calcitic hard parts, echinoderms are especially vulnerable to dissolution stress from ocean acidification because they currently inhabit seawater that is barely at the saturation level to support biogenic calcification. Thus, cold-water, high latitude species with a high Mg-content in their hard parts are considered to be the \u0027first responders\u0027 to chemical changes in the surface oceans. Studies in this proposal will use several metrics to examine the physiological plasticity of contemporary urchin embryos and larvae to CO2-acidified seawater, to mimic the scenarios defined by IPCC models and by analyses of future acidification predicted for the Southern Ocean. The research also will investigats the biological consequences of synergistic interactions of two converging climate change-related stressors - CO2- driven ocean acidification and ocean warming. Specifically the research will (1) assess the effect of CO2-acidified seawater on the development of early embryos and larvae, (2) using morphometrics, examine changes in the larval endoskeleton in response to development under the high-CO2 conditions of ocean acidification, (3) using a DNA microarray, profile changes in gene expression for genes involved in biomineralization and other important physiological processes, and (4) measure costs and physiological consequences of development under conditions of ocean acidification. The proposal will support the training of undergraduates, graduate students and a postdoctoral fellow. The PI also will collaborate with the UC Santa Barbara Gevirtz Graduate School of Education to link the biological effects of ocean acidification to the chemical changes expected for the Southern Ocean using the \u0027Science on a Sphere\u0027 technology. This display will be housed in an education and public outreach center, the Outreach Center for Teaching Ocean Science (OCTOS), a new state-of-the-art facility under construction at UC Santa Barbara.", "east": -150.0, "geometry": "POINT(-175 -73)", "instruments": null, "is_usap_dc": true, "keywords": "MOORINGS", "locations": null, "north": -68.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Hofmann, Gretchen", "platforms": "WATER-BASED PLATFORMS \u003e BUOYS \u003e MOORED \u003e MOORINGS", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -78.0, "title": "Effect of Ocean Acidification on Early Life History Stages of the Antarctic Sea Urchins Sterechinus Neumayeri", "uid": "p0000352", "west": 160.0}, {"awards": "1043745 Halanych, Kenneth", "bounds_geometry": null, "dataset_titles": "Expedition Data", "datasets": [{"dataset_uid": "001427", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/NBP1210"}, {"dataset_uid": "000439", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG1312"}], "date_created": "Fri, 07 Feb 2014 00:00:00 GMT", "description": "The research will explore the genetics, diversity, and biogeography of Antarctic marine benthic invertebrates, seeking to overturn the widely accepted suggestion that benthic fauna do not constitute a large, panmictic population. The investigators will sample adults and larvae from undersampled regions of West Antarctica that, combined with existing samples, will provide significant coverage of the western hemisphere of the Southern Ocean. The objectives are: 1) To assess the degree of genetic connectivity (or isolation) of benthic invertebrate species in the Western Antarctic using high-resolution genetic markers. 2) To begin exploring planktonic larvae spatial and bathymetric distributions for benthic shelf invertebrates in the Bellinghausen, Amundsen and Ross Seas. 3) To continue to develop a Marine Antarctic Genetic Inventory (MAGI) that relates larval and adult forms via DNA barcoding. Broader impacts include traditional forms of training (postdocs, graduate studentships, undergraduate research experiences) and lectures to K-12 groups.", "east": null, "geometry": null, "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e CHEMICAL METERS/ANALYZERS \u003e FLUOROMETERS; IN SITU/LABORATORY INSTRUMENTS \u003e RECORDERS/LOGGERS \u003e AWS; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e XBT; EARTH REMOTE SENSING INSTRUMENTS \u003e ACTIVE REMOTE SENSING \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ECHO SOUNDERS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e THERMOSALINOGRAPHS; IN SITU/LABORATORY INSTRUMENTS \u003e MAGNETIC/MOTION SENSORS \u003e GRAVIMETERS \u003e GRAVIMETERS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e PROFILERS; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e XBT; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e MBES", "is_usap_dc": false, "keywords": "R/V NBP; R/V LMG", "locations": null, "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Halanych, Kenneth", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP; WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "R2R", "repositories": "Other", "science_programs": null, "south": null, "title": "Collaborative Research: Genetic connectivity and biogeographic patterns of Antarctic benthic invertebrates", "uid": "p0000263", "west": null}, {"awards": "1142107 Durbin, Edward", "bounds_geometry": null, "dataset_titles": "Expedition data of NBP1304", "datasets": [{"dataset_uid": "002660", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of NBP1304", "url": "https://www.rvdata.us/search/cruise/NBP1304"}], "date_created": "Fri, 07 Feb 2014 00:00:00 GMT", "description": "Krill, Euphausia superba, is a keystone species in the Antarctic ecosystem and provides the trophic link between microscopic plankton and charismatic megafauna such as penguins and whales. Recent evidence suggests krill may not be exclusively planktonivorous, which introduces the potential of new pathways of carbon flux through krill based ecosystems. A change in our view of krill from one of being herbivores to omnivores opens up several questions.\u003cbr/\u003e\u003cbr/\u003eClimate induced change in the extent, thickness and duration of overlying sea ice coverage is expected to change the prey fields available to krill, and to have subsequent effects on the suite of predators supported by krill. The nature of this benthic prey?krill link, which may be crucial in those parts of the seasonal cycle other than the well studied spring bloom, is yet to be determined. DNA techniques will be used to identify and quantify the prey organisms. \u003cbr/\u003e\u003cbr/\u003eThis project will measure the in situ feeding ecology and behavior of krill and, ultimately, the success of this key species. An overall goal is\u003cbr/\u003eto investigate seasonal changes in Euphausia superba in-situ feeding and swimming behavior in the Wilhelmina Bay region of the Western Antarctic Peninsula (WAP) area, known to be a region of changing climate. Understanding the biological impacts of climate change is important to societal and economic goals. The project scientists will additionally team with a marine and environmental reporting group to design presentations for an annual journalist meeting.", "east": null, "geometry": null, "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; IN SITU/LABORATORY INSTRUMENTS \u003e CHEMICAL METERS/ANALYZERS \u003e FLUOROMETERS; IN SITU/LABORATORY INSTRUMENTS \u003e MAGNETIC/MOTION SENSORS \u003e GRAVIMETERS \u003e GRAVIMETERS; IN SITU/LABORATORY INSTRUMENTS \u003e RECORDERS/LOGGERS \u003e AWS; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e XBT; EARTH REMOTE SENSING INSTRUMENTS \u003e ACTIVE REMOTE SENSING \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ECHO SOUNDERS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e MBES; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e THERMOSALINOGRAPHS", "is_usap_dc": false, "keywords": "R/V NBP", "locations": null, "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Instrumentation and Support", "paleo_time": null, "persons": "Durbin, Edward", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repo": "R2R", "repositories": "Other", "science_programs": null, "south": null, "title": "Collaborative Research: Seasonal Trophic Roles of Euphausia Superba (STRES)", "uid": "p0000848", "west": null}, {"awards": "0739648 Cary, Stephen", "bounds_geometry": "POINT(163 -77.5)", "dataset_titles": "Biogeochemistry of Cyanobactrial Mats and Hyporheic Zone Microbes in McMurdo Dry Valley Glacial Meltwater Streams", "datasets": [{"dataset_uid": "600079", "doi": "10.15784/600079", "keywords": "Antarctica; Biology; Biosphere; Cell Counts; Cryosphere; Dry Valleys; Microbiology", "people": "Cary, S. Craig", "repository": "USAP-DC", "science_program": null, "title": "Biogeochemistry of Cyanobactrial Mats and Hyporheic Zone Microbes in McMurdo Dry Valley Glacial Meltwater Streams", "url": "https://www.usap-dc.org/view/dataset/600079"}], "date_created": "Tue, 10 Dec 2013 00:00:00 GMT", "description": "The glacial streams of the McMurdo Dry Valleys have extensive cyanobacterial mats that are a probable source of fixed C and N to the Valleys. The research will examine the interplay between the microbial mats in the ephemeral glacial streams and the microbiota of the hyporheic soils (wetted soil zone) underlying and adjacent to those mats. It is hypothesized that the mats are important sources of organic carbon and fixed nitrogen for the soil communities of the hyporheic zone, and release dissolved organic carbon (DOC) and nitrogen (DON) that serves the entire Dry Valley ecosystem. Field efforts will entail both observational and experimental components. Direct comparisons will be made between the mats and microbial populations underlying naturally rehydrated and desiccated mat areas, and between mat areas in the melt streams of the Adams and Miers Glaciers in Miers Valley. Both physiological and phylogenetic indices of the soil microbiota will be examined. Observations will include estimates of rates of mat carbon and nitrogen fixation, soil respiration and leucine and thymidine uptake (as measures of protein \u0026 DNA synthesis, respectively) by soil bacteria, bacterial densities and their molecular ecology. Experimental manipulations will include experimental re-wetting of soils and observations of the time course of response of the microbial community. The research will integrate modern molecular genetic approaches (ARISA-DNA fingerprinting and ultra deep 16S rDNA microbial phylogenetic analysis) with geochemistry to study the diversity, ecology, and function of microbial communities that thrive in these extreme environments. The broader impacts of the project include research and educational opportunities for graduate students and a postdoctoral associate. The P.I.s will involve undergraduates as work-study students and in REU programs, and will participate in educational and outreach programs.", "east": 163.0, "geometry": "POINT(163 -77.5)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -77.5, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Cary, Stephen", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -77.5, "title": "Collaborative Research: Biogeochemistry of Cyanobactrial Mats and Hyporheic Zone Microbes in McMurdo Dry Valley Glacial Meltwater Streams", "uid": "p0000476", "west": 163.0}, {"awards": "0739783 Junge, Karen", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "Metabolic Activities and Gene Expression of Marine Psychrophiles in Cold Ice", "datasets": [{"dataset_uid": "600083", "doi": "10.15784/600083", "keywords": "Antarctica; Biology; Biosphere; Cryosphere; Microbiology; Oceans; Sea Ice; Southern Ocean", "people": "Junge, Karen", "repository": "USAP-DC", "science_program": null, "title": "Metabolic Activities and Gene Expression of Marine Psychrophiles in Cold Ice", "url": "https://www.usap-dc.org/view/dataset/600083"}], "date_created": "Wed, 25 Sep 2013 00:00:00 GMT", "description": "The mechanisms enabling bacteria to be metabolically active at very low temperatures are of considerable importance to polar microbial ecology, astrobiology, climate and cryopreservation. This research program has two main objectives. The first is to investigate metabolic activities and gene expression of polar marine psychrophilic bacteria when confronted with freezing conditions at temperatures above the eutectic of seawater (\u003c54C) to unveil cold adaptation mechanisms with relevance to wintertime sea-ice ecology. The second objective is to discern if psychrophilic processes of leucine incorporation into proteins, shown to occur to -196C, amount to metabolic activity providing for the survival of cells or are merely biochemical reactions still possible in flash-frozen samples without any effect on survival. We will examine extracellular and intracellular processes of psychrophilic activity above and below the eutectic by (i) determining the temperature range of metabolic activities such as DNA synthesis, carbon utilization, respiration and ATP generation using radioactive tracer technology, including a control at liquid helium temperature (-268.9C), (ii) analyzing gene expression in ice using whole genome and microarray analyses and iii) examining the role of exopolymeric substances (EPS) and ice micro-physics for the observed activity using an in-situ microscopy technique. Results of the proposed research can be expected to aid in the determination of cellular and genetic strategies that allow cells to maintain activity at extremely low temperatures within an icy matrix and/or to resume activity again when more growth-permissive conditions are encountered. The research is an interdisciplinary collaboration involving three different institutions with participants in Oceanography, Genomics, and Geophysical Sciences. The proposed activity will support the beginning professional career of a female researcher and will serve as the basis for several undergraduate student laboratory projects.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Junge, Karen", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -90.0, "title": "Metabolic Activities and Gene Expression of Marine Psychrophiles in Cold Ice", "uid": "p0000673", "west": -180.0}, {"awards": "0439906 Koch, Paul", "bounds_geometry": "POLYGON((162 -72,162.6 -72,163.2 -72,163.8 -72,164.4 -72,165 -72,165.6 -72,166.2 -72,166.8 -72,167.4 -72,168 -72,168 -72.6,168 -73.2,168 -73.8,168 -74.4,168 -75,168 -75.6,168 -76.2,168 -76.8,168 -77.4,168 -78,167.4 -78,166.8 -78,166.2 -78,165.6 -78,165 -78,164.4 -78,163.8 -78,163.2 -78,162.6 -78,162 -78,162 -77.4,162 -76.8,162 -76.2,162 -75.6,162 -75,162 -74.4,162 -73.8,162 -73.2,162 -72.6,162 -72))", "dataset_titles": "Abandoned Elephant Seal Colonies in Antarctica: Integration of Genetic, Isotopic, and Geologic Approaches toward Understanding Holocene Environmental Change", "datasets": [{"dataset_uid": "600041", "doi": "10.15784/600041", "keywords": "Biology; Biosphere; Isotope; Penguin; Ross Sea; Seals; Southern Ocean", "people": "Koch, Paul", "repository": "USAP-DC", "science_program": null, "title": "Abandoned Elephant Seal Colonies in Antarctica: Integration of Genetic, Isotopic, and Geologic Approaches toward Understanding Holocene Environmental Change", "url": "https://www.usap-dc.org/view/dataset/600041"}], "date_created": "Sat, 30 Oct 2010 00:00:00 GMT", "description": "During previous NSF-sponsored research, the PI\u0027s discovered that southern elephant seal colonies once existed along the Victoria Land coast (VLC) of Antarctica, a region where they are no longer observed. Molted seal skin and hair occur along 300 km of coastline, more than 1000 km from any extant colony. The last record of a seal at a former colony site is at ~A.D. 1600. Because abandonment occurred prior to subantarctic sealing, disappearance of the VLC colony probably was due to environmental factors, possibly cooling and encroachment of land-fast, perennial sea ice that made access to haul-out sites difficult. The record of seal inhabitation along the VLC, therefore, has potential as a proxy for climate change. Elephant seals are a predominantly subantarctic species with circumpolar distribution. Genetic studies have revealed significant differentiation among populations, particularly with regard to that at Macquarie I., which is the extant population nearest to the abandoned VLC colony. Not only is the Macquarie population unique genetically, but it is has undergone unexplained decline of 2%/yr over the last 50 years3. In a pilot study, genetic analyses showed a close relationship between the VLC seals and those at Macquarie I. An understanding of the relationship between the two populations, as well as of the environmental pressures that led to the demise of the VLC colonies, will provide a better understanding of present-day population genetic structure, the effect of environmental change on seal populations, and possibly the reasons underlying the modern decline at Macquarie Island.\u003cbr/\u003eThis project addresses several key research problems: (1) Why did elephant seals colonize and then abandon the VLC? (2) What does the elephant seal record reveal about Holocene climate change and sea-ice conditions? (3) What were the foraging strategies of the seals and did these strategies change over time as climate varied? (4) How does the genetic structure of the VLC seals relate to extant populations? (5) How did genetic diversity change over time and with colony decline? (6) Using ancient samples to estimate mtDNA mutation rates, what can be learned about VLC population dynamics over time? (7) What was the ecological relationship between elephant seals and Adelie penguins that occupied the same sites, but apparently at different times? The proposed work includes the professional training of young researchers and incorporation of data into graduate and undergraduate courses.", "east": 168.0, "geometry": "POINT(165 -75)", "instruments": null, "is_usap_dc": true, "keywords": "FIELD SURVEYS", "locations": null, "north": -72.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Earth Sciences", "paleo_time": null, "persons": "Koch, Paul", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -78.0, "title": "Collaborative Research: Abandoned Elephant Seal Colonies in Antarctica: Integration of Genetic, Isotopic, and Geologic Approaches toward Understanding Holocene Environmental Change", "uid": "p0000533", "west": 162.0}, {"awards": "0636696 DeVries, Arthur", "bounds_geometry": "POLYGON((-68.0025 -52.7599,-67.07254 -52.7599,-66.14258 -52.7599,-65.21262 -52.7599,-64.28266 -52.7599,-63.3527 -52.7599,-62.42274 -52.7599,-61.49278 -52.7599,-60.56282 -52.7599,-59.63286 -52.7599,-58.7029 -52.7599,-58.7029 -53.98242,-58.7029 -55.20494,-58.7029 -56.42746,-58.7029 -57.64998,-58.7029 -58.8725,-58.7029 -60.09502,-58.7029 -61.31754,-58.7029 -62.54006,-58.7029 -63.76258,-58.7029 -64.9851,-59.63286 -64.9851,-60.56282 -64.9851,-61.49278 -64.9851,-62.42274 -64.9851,-63.3527 -64.9851,-64.28266 -64.9851,-65.21262 -64.9851,-66.14258 -64.9851,-67.07254 -64.9851,-68.0025 -64.9851,-68.0025 -63.76258,-68.0025 -62.54006,-68.0025 -61.31754,-68.0025 -60.09502,-68.0025 -58.8725,-68.0025 -57.64998,-68.0025 -56.42746,-68.0025 -55.20494,-68.0025 -53.98242,-68.0025 -52.7599))", "dataset_titles": "Expedition Data; Expedition data of LMG0809", "datasets": [{"dataset_uid": "001504", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0809"}, {"dataset_uid": "001493", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0810"}, {"dataset_uid": "002728", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0809", "url": "https://www.rvdata.us/search/cruise/LMG0809"}], "date_created": "Tue, 04 May 2010 00:00:00 GMT", "description": "Antarctic notothenioid fish evolved antifreeze (AF) proteins that prevent ice crystals that enter their body fluids from growing, and thereby avoid freezing in their icy habitats. However, even in the extreme cold Antarctic marine environment, regional gradations of severity are found. The biological correlate for environmental severity in fish is the endogenous ice load, which likely determines the tolerable limit of environmental severity for notothenioid habitation. The endogenous ice load develops from environmental ice crystals entering through body surfaces and somehow localizing to the spleen. How prone the surface tissues are to ice entry, how ice reaches the spleen, and what the fate of splenic ice is, requires elucidation. Spleen sequestration of ice raises the hypothesis that macrophages may play a role in the translocation and perhaps elimination of AF-bound ice crystals. Antifreeze glycoproteins (AFGP) act in concert with a second, recently discovered antifreeze called antifreeze potentiating protein (AFPP), necessitating an assessment of the contribution of AFPP to freezing avoidance. Recent research suggests that the exocrine pancreas and the anterior stomach, not the liver, synthesize AFGPs and secrete them into the intestine, from where they may be returned to the blood. A GI-to-blood transport is a highly unconventional path for a major plasma protein and also begs the questions, What is the source of blood AFPP?. Why are two distinct AF proteins needed and what is the chronology of their evolution? What genomic changes in the DNA are associated with the development or loss of the antifreeze trait? Experiments described in this proposal address these interrelated questions of environmental, organismal, and evolutionary physiology, and will further our understanding of novel vertebrate physiologies, the limits of environmental adaptation, and climatically driven changes in the genome. The proposed research will (1) determine the temporal and spatial heterogeneity of environmental temperature and iciness in progressively more severe fish habitats in the greater McMurdo Sound area, and in the milder Arthur Harbor at Palmer Station. The splenic ice load in fishes inhabiting these sites will be determined to correlate to environmental severity and habitability. (2) Assess the surface tissue site of ice entry and their relative barrier properties in intact fish and isolated tissues preparations (3) Assess the role of immune cells in the fate of endogenous ice, (4) determine whether the blood AFGPs are from intestinal/rectal uptake, (5) examine the contribution of AFPP to the total blood AF activity (6) evaluate the progression of genomic changes in the AFGP locus across Notothenioidei as modulated by disparate thermal environments, in four selected species through the analyses of large insert DNA BAC clones. The origin and evolution of AFPP will be examined also by analyzing BAC clones encompassing the AFPP genomic locus. The broader impacts of the proposed research include training of graduate and undergraduate students in research approaches ranging from physical field measurements to cutting edge genomics. Undergraduate research projects have lead to co-authored publications and will continue to do so. Outreach includes establishing Wiki websites on topics of Antarctic fish biology and freeze avoidance, providing advisory services to the San Francisco Science Exploratorium, and making BAC libraries available to interested polar biologists. This research theme has repeatedly received national and international science news coverage and will continue to be disseminated to the public in that manner.", "east": -58.7029, "geometry": "POINT(-63.3527 -58.8725)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ADCP", "is_usap_dc": false, "keywords": "R/V LMG", "locations": null, "north": -52.7599, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Devries, Arthur", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "R2R", "repositories": "Other", "science_programs": null, "south": -64.9851, "title": "Environmental, Organismal and Evolutionary Physiology of Freeze Avoidance in Antarctic Notothenioid Fishes", "uid": "p0000560", "west": -68.0025}, {"awards": "0125818 Gargett, Ann", "bounds_geometry": null, "dataset_titles": "Expedition Data; Expedition data of NBP0508", "datasets": [{"dataset_uid": "002610", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of NBP0508", "url": "https://www.rvdata.us/search/cruise/NBP0508"}, {"dataset_uid": "001584", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/NBP0508"}], "date_created": "Tue, 04 May 2010 00:00:00 GMT", "description": "Ultraviolet radiation influences the dynamics of plankton processes in the near-surface waters of most aquatic ecosystems. In particular, the Southern Ocean is affected in the austral spring period when biologically damaging ultraviolet radiation is enhanced by ozone depletion. While progress has been made in estimating the quantitative impact of ultraviolet radiation on bacteria and phytoplankton in the Southern Ocean, some important issues remain to be resolved. Little is known about responses in systems dominated by the colonial haptophyte Phaeocystis antarctica, which dominates spring blooms in a polyna that develops in the southern Ross Sea. The Ross Sea is also of interest because of the occurrence of open water at a far southerly location in the spring, well within the ozone hole, and continuous daylight, with implications for the regulation of DNA repair. A number of studies suggest that vertical mixing can significant modify the impact of ultraviolet radiation in the Southern Ocean and elsewhere. However, there are limited measurements of turbulence intensity in the surface layer and measurements have not been integrated with parallel studies of ultraviolet radiation effects on phytoplankton and bacterioplankton. To address these issues, this collaborative study will focus on vertical mixing and the impact of ultraviolet radiation in the Ross Sea. The spectral and temporal responses of phytoplankton and bacterioplankton to ultraviolet radiation will be characterized in both laboratory and solar incubations. These will lead to the definition of biological weighting functions and response models capable of predicting the depth and time distribution of ultraviolet radiation impacts on photosynthesis, bacterial incorporation and DNA damage in the surface layer. Diel sampling will measure depth-dependent profiles of DNA damage, bacterial incorporation, photosynthesis and fluorescence parameters over a 24 h cycle. Sampling will include stations with contrasting wind-driven mixing and stratification as the polyna develops. The program of vertical mixing measurements is optimized for the typical springtime Ross Sea situation in which turbulence of intermediate intensity is insufficient to mix the upper layer thoroughly in the presence of stabilizing influences like solar heating and/or surface freshwater input from melting ice. Fine-scale vertical density profiles will be measured with a free-fall CTD unit and the profiles will be used to directly estimate large-eddy scales by determining Thorpe scales. Eddy scales and estimated turbulent diffusivities will be directly related to surface layer effects, and used to generate lagrangian depth-time trajectories in models of ultraviolet radiation responses in the surface mixed layer. The proposed research will be the first in-depth study of ultraviolet radiation effects in the Ross Sea and provide a valuable comparison with previous work in the Weddell-Scotia Confluence and Palmer Station regions. It will also enhance the understanding of vertical mixing processes, trophic interactions and biogeochemical cycling in the Ross Sea.", "east": null, "geometry": null, "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; IN SITU/LABORATORY INSTRUMENTS \u003e MAGNETIC/MOTION SENSORS \u003e GRAVIMETERS \u003e GRAVIMETERS; IN SITU/LABORATORY INSTRUMENTS \u003e PRESSURE/HEIGHT METERS \u003e PRESSURE SENSORS; IN SITU/LABORATORY INSTRUMENTS \u003e PHOTON/OPTICAL DETECTORS \u003e TURBIDITY METERS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ADCP", "is_usap_dc": false, "keywords": "B-15J; R/V NBP", "locations": null, "north": null, "nsf_funding_programs": "Antarctic Ocean and Atmospheric Sciences", "paleo_time": null, "persons": "Neale, Patrick", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repo": "R2R", "repositories": "Other", "science_programs": null, "south": null, "title": "Collaborative Research: Interactive effects of UV and vertical mixing on phytoplankton and bacterioplankton in the Ross Sea", "uid": "p0000822", "west": null}, {"awards": "0125890 Sidell, Bruce", "bounds_geometry": "POLYGON((-68.1413 -52.6755,-67.47503 -52.6755,-66.80876 -52.6755,-66.14249 -52.6755,-65.47622 -52.6755,-64.80995 -52.6755,-64.14368 -52.6755,-63.47741 -52.6755,-62.81114 -52.6755,-62.14487 -52.6755,-61.4786 -52.6755,-61.4786 -53.8957,-61.4786 -55.1159,-61.4786 -56.3361,-61.4786 -57.5563,-61.4786 -58.7765,-61.4786 -59.9967,-61.4786 -61.2169,-61.4786 -62.4371,-61.4786 -63.6573,-61.4786 -64.8775,-62.14487 -64.8775,-62.81114 -64.8775,-63.47741 -64.8775,-64.14368 -64.8775,-64.80995 -64.8775,-65.47622 -64.8775,-66.14249 -64.8775,-66.80876 -64.8775,-67.47503 -64.8775,-68.1413 -64.8775,-68.1413 -63.6573,-68.1413 -62.4371,-68.1413 -61.2169,-68.1413 -59.9967,-68.1413 -58.7765,-68.1413 -57.5563,-68.1413 -56.3361,-68.1413 -55.1159,-68.1413 -53.8957,-68.1413 -52.6755))", "dataset_titles": "Expedition Data; Expedition data of LMG0304; Expedition data of LMG0304A", "datasets": [{"dataset_uid": "001704", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0304"}, {"dataset_uid": "002708", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0304A", "url": "https://www.rvdata.us/search/cruise/LMG0304A"}, {"dataset_uid": "002707", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0304A", "url": "https://www.rvdata.us/search/cruise/LMG0304A"}, {"dataset_uid": "002706", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0304", "url": "https://www.rvdata.us/search/cruise/LMG0304"}, {"dataset_uid": "001596", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0506"}, {"dataset_uid": "001597", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0505"}], "date_created": "Tue, 04 May 2010 00:00:00 GMT", "description": "Notothenioid fishes that dominate the fish fauna surrounding Antarctica have been evolving for 10-14 million years at a nearly constant body temperature of ~0C throughout their life histories. As a result, this group of animals is uniquely suited to studies aimed at understanding and identifying features of physiology and biochemistry that result from the process of evolution at cold body temperature. This project has three major objectives aimed at examining adaptations for life in cold environments: \u003cbr/\u003e\u003cbr/\u003e1. Identify the amino acid substitutions in the fatty acid-binding pocket of fatty acyl CoA synthetase (FACS) that explain its substrate specificity. Fatty acids are a major fuel of energy metabolism in Antarctic fishes. FACS catalyzes the condensation of CoASH and fatty acids to fatty acyl CoA esters, a step required for subsequent metabolism of these important compounds. This research may permit us to resolve the specific amino acid substitutions that explain both substrate specificity and preservation of catalytic rate of notothenioid FACS at cold physiological temperatures.\u003cbr/\u003e\u003cbr/\u003e2. Produce a rigorous biochemical and biophysical characterization of the intracellular calcium-binding protein, parvalbumin, from white axial musculature of Antarctic fishes. Parvalbumin plays a pivotal role in facilitating the relaxation phase of fast-contracting muscles and is a likely site of strong selective pressure. Preliminary data strongly indicate that the protein from Antarctic fishes has been modified to ensure function at cold temperature. A suite of physical techniques will be used to determine dissociation constants of Antarctic fish parvalbumins for calcium and magnesium and unidirectional rate constants of ion-dissociation from the protein. Full-length cDNA clones for Antarctic fish parvalbumin(s) will permit deduction of primary amino acid sequence These data will yield insight into structural elements that permit the protein from notothenioid fishes to function at very cold body temperature.\u003cbr/\u003e\u003cbr/\u003e3. Conduct a broad survey of the pattern of cardiac myoglobin expression in the Suborder Notothenoidei. Previous work has indicated a variable pattern of presence or absence of the intracellular oxygen-binding protein, myoglobin (Mb), in hearts of one family of Antarctic notothenioid fishes (Channichthyidae; icefishes). Because Mb is of physiological value in species that express the protein, the observed pattern of interspecific expression has been attributed to unusually low niche competition in the Southern Ocean. This leads to the prediction that similar loss of cardiac Mb should be observed in other notothenioid taxa. This part of the project will survey for the presence and absence of cardiac Mb in as many notothenioid species as possible and, if Mb-lacking species are detected, will extend analyses to determine the mechanism(s) responsible for loss of its expression using molecular biological techniques.", "east": -61.4786, "geometry": "POINT(-64.80995 -58.7765)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PRESSURE/HEIGHT METERS \u003e PRESSURE SENSORS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ADCP", "is_usap_dc": false, "keywords": "R/V LMG", "locations": null, "north": -52.6755, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Sidell, Bruce; Detrich, H. William", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "R2R", "repositories": "Other", "science_programs": null, "south": -64.8775, "title": "Cold Body Temperature as an Evolutionary Shaping force in the Physiology of Antarctic Fishes", "uid": "p0000241", "west": -68.1413}, {"awards": "9714299 Caron, David", "bounds_geometry": "POLYGON((-179.9984 -43.56676,-143.99865 -43.56676,-107.9989 -43.56676,-71.99915 -43.56676,-35.9994 -43.56676,0.000349999999997 -43.56676,36.0001 -43.56676,71.99985 -43.56676,107.9996 -43.56676,143.99935 -43.56676,179.9991 -43.56676,179.9991 -47.057693,179.9991 -50.548626,179.9991 -54.039559,179.9991 -57.530492,179.9991 -61.021425,179.9991 -64.512358,179.9991 -68.003291,179.9991 -71.494224,179.9991 -74.985157,179.9991 -78.47609,143.99935 -78.47609,107.9996 -78.47609,71.99985 -78.47609,36.0001 -78.47609,0.000349999999997 -78.47609,-35.9994 -78.47609,-71.99915 -78.47609,-107.9989 -78.47609,-143.99865 -78.47609,-179.9984 -78.47609,-179.9984 -74.985157,-179.9984 -71.494224,-179.9984 -68.003291,-179.9984 -64.512358,-179.9984 -61.021425,-179.9984 -57.530492,-179.9984 -54.039559,-179.9984 -50.548626,-179.9984 -47.057693,-179.9984 -43.56676))", "dataset_titles": "Expedition Data", "datasets": [{"dataset_uid": "002003", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/NBP9901"}], "date_created": "Tue, 04 May 2010 00:00:00 GMT", "description": "*** Caron 9714299 The analysis of microbial biodiversity of extreme environments is difficult because traditional methods for examining diversity are often ineffective for assessing species richness within these communities. Additional difficulties arise due to the difficulties of recreating and maintaining pertinent environmental features during sample collection and procession. This study focuses on the protistan assemblages (algae and protozoa) in the sea ice, sediment and ocean environments of the Ross Sea, Antarctica. The identification of protistan species in natural assemblages traditionally has entailed direct microscopical analyses as well as enrichment and culture techniques for assessing biodiversity. Determination of diversity for these assemblages in therefore susceptible to biases as a consequence of sampling, enrichment and culture, as well as selective losses due to sample preservation and concentration for microscopy. The goals of this project are: (1) to develop and apply molecular biological approaches to assess species diversity of small protists (algae and protozoa smaller than 100 micrometers) in ocean water, sea ice and sediment environments and (2) to obtain baseline physiological information on the growth rates, feeding rates and growth efficiencies of cultured protozoa under pertinent temperature regimes. Molecular biological studies will involve the use of PCR-based protocols to examine small subunit ribosomal RNA gene (srDNA) diversity. Approaches and techniques developed will be applicable to any other water body or sediment and would provide a means to examine the representativeness of protistan cultures in extant culture collections. ***", "east": 179.9991, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": false, "keywords": "R/V NBP", "locations": null, "north": -43.56676, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Caron, David; Jeffries, Martin", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repo": "R2R", "repositories": "Other", "science_programs": null, "south": -78.47609, "title": "LEXEN: Protistan Biodiversity in Antarctic Marine Ecosystems: Molecular Biological and Traditional Approaches", "uid": "p0000625", "west": -179.9984}, {"awards": "0632250 Cary, Stephen", "bounds_geometry": "POLYGON((-38.5 -72.6,-23.963 -72.6,-9.426 -72.6,5.111 -72.6,19.648 -72.6,34.185 -72.6,48.722 -72.6,63.259 -72.6,77.796 -72.6,92.333 -72.6,106.87 -72.6,106.87 -73.185,106.87 -73.77,106.87 -74.355,106.87 -74.94,106.87 -75.525,106.87 -76.11,106.87 -76.695,106.87 -77.28,106.87 -77.865,106.87 -78.45,92.333 -78.45,77.796 -78.45,63.259 -78.45,48.722 -78.45,34.185 -78.45,19.648 -78.45,5.111 -78.45,-9.426 -78.45,-23.963 -78.45,-38.5 -78.45,-38.5 -77.865,-38.5 -77.28,-38.5 -76.695,-38.5 -76.11,-38.5 -75.525,-38.5 -74.94,-38.5 -74.355,-38.5 -73.77,-38.5 -73.185,-38.5 -72.6))", "dataset_titles": "Metagenomic Data Lake Vostok Microbial Community", "datasets": [{"dataset_uid": "000136", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Metagenomic Data Lake Vostok Microbial Community", "url": "http://www.ncbi.nlm.nih.gov/genbank/"}], "date_created": "Fri, 30 Apr 2010 00:00:00 GMT", "description": "This project brings together researchers with expertise in molecular microbial ecology, Antarctic and deep sea environments, and metagenomics to address the overarching question: how do ecosystems dominated by microorganisms adapt to conditions of continuous cold and dark over evolutionarily and geologically relevant time scales? Lake Vostok, buried for at least 15 million years beneath approximately 4 km of ice that has prevented any communication with the external environment for as much as 1.5 million years, is an ideal system to study this question. Water from the lake that has frozen on to the bottom of the ice sheet (accretion ice) is available for study. Several studies have indicated the presence of low abundance, but detectable microbial communities in the accretion ice. Our central hypothesis maintains that Lake Vostok microbes are specifically adapted to life in conditions of extreme cold, dark, and oligotrophy and that signatures of those adaptations can be observed in their genome sequences at the gene, organism, and community levels. To address this hypothesis, we propose to characterize the metagenome (i.e. the genomes of all members of the community) of the accretion ice. using whole genome amplification (WGA), which can provide micrograms of unbiased metagenomic DNA from only a few cells. The results of this project have relevance to evolutionary biology and ecology, subglacial Antarctic lake exploration, biotechnology, and astrobiology. The project directly addresses priorities and themes in the International Polar Year at the national and international levels. A legacy of DNA sequence data and the metagenomic library will be created and maintained. Press releases and a publicly available web page will facilitate communication with the public. K-12 outreach will be the focus of a new, two-tiered program targeting the 7th grade classroom and on site visits to the Joint Genome Institute Production Sequencing Facility by high school juniors and seniors and community college level students. Minority undergraduate researchers will be recruited for research on this project, and support and training are provided to two graduate students, a postdoctoral scholar, and a technician.", "east": 106.87, "geometry": "POINT(34.185 -75.525)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -72.6, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Cary, Stephen", "platforms": "Not provided", "repo": "NCBI GenBank", "repositories": "Other", "science_programs": null, "south": -78.45, "title": "IPY: Collaborative Research: A Metagenomic Investigation of Adaptation to Prolonged Cold and Dark Conditions of the Lake Vostok Microbial Community", "uid": "p0000201", "west": -38.5}, {"awards": "0808947 Hofmann, Gretchen", "bounds_geometry": "POLYGON((-180 -70,-177 -70,-174 -70,-171 -70,-168 -70,-165 -70,-162 -70,-159 -70,-156 -70,-153 -70,-150 -70,-150 -70.7,-150 -71.4,-150 -72.1,-150 -72.8,-150 -73.5,-150 -74.2,-150 -74.9,-150 -75.6,-150 -76.3,-150 -77,-153 -77,-156 -77,-159 -77,-162 -77,-165 -77,-168 -77,-171 -77,-174 -77,-177 -77,180 -77,178 -77,176 -77,174 -77,172 -77,170 -77,168 -77,166 -77,164 -77,162 -77,160 -77,160 -76.3,160 -75.6,160 -74.9,160 -74.2,160 -73.5,160 -72.8,160 -72.1,160 -71.4,160 -70.7,160 -70,162 -70,164 -70,166 -70,168 -70,170 -70,172 -70,174 -70,176 -70,178 -70,-180 -70))", "dataset_titles": "Science of Opportunity: A SGER proposal to support the development of genomic resources for Antarctic pteropods", "datasets": [{"dataset_uid": "600088", "doi": "10.15784/600088", "keywords": "Biology; Biosphere; Genomics; Oceans; Southern Ocean", "people": "Fabry, Victoria; Hofmann, Gretchen", "repository": "USAP-DC", "science_program": null, "title": "Science of Opportunity: A SGER proposal to support the development of genomic resources for Antarctic pteropods", "url": "https://www.usap-dc.org/view/dataset/600088"}], "date_created": "Sat, 20 Feb 2010 00:00:00 GMT", "description": "This Small Grant for Exploratory Research (SGER) will support the rapid acquisition of DNA sequence for the Antarctic pteropod Limacina helicina, a resource that would allow the development of a cDNA microarray to profile gene expression in this critical marine invertebrate in response to ocean acidification. This request would facilitate the collaboration of the PI (Hofmann), a marine molecular ecologist, with co-PI, Prof. Victoria Fabry, an expert in pteropod calcification biology, and a leader in the ocean acidification research community. Finally, the resources developed here would be shared with the polar research community and all DNA sequence data and protocols would be available via web databases. Notably, the genomic tool developed here would most likely be useful for pteropods from Antarctic and Arctic waters. The broader impacts of this project would be the development of genomic tools for a critical Antarctic marine invertebrate that is threatened by ocean acidification. In addition, these resources would be shared with the polar biology research community.", "east": -150.0, "geometry": "POINT(-175 -73.5)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -70.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Hofmann, Gretchen; Fabry, Victoria", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -77.0, "title": "Science of Opportunity: A SGER proposal to support the development of genomic resources for Antarctic pteropods", "uid": "p0000213", "west": 160.0}, {"awards": "0125098 Emslie, Steven", "bounds_geometry": "POLYGON((-50 -60,-29 -60,-8 -60,13 -60,34 -60,55 -60,76 -60,97 -60,118 -60,139 -60,160 -60,160 -63,160 -66,160 -69,160 -72,160 -75,160 -78,160 -81,160 -84,160 -87,160 -90,139 -90,118 -90,97 -90,76 -90,55 -90,34 -90,13 -90,-8 -90,-29 -90,-50 -90,-50 -87,-50 -84,-50 -81,-50 -78,-50 -75,-50 -72,-50 -69,-50 -66,-50 -63,-50 -60))", "dataset_titles": "Occupation History and Diet of Adelie Penguins in the Ross Sea Region", "datasets": [{"dataset_uid": "600028", "doi": "10.15784/600028", "keywords": "Antarctica; Biology; Biosphere; Geochronology; Oceans; Paleoclimate; Penguin; Radiocarbon; Ross Sea; Southern Ocean", "people": "Emslie, Steven", "repository": "USAP-DC", "science_program": null, "title": "Occupation History and Diet of Adelie Penguins in the Ross Sea Region", "url": "https://www.usap-dc.org/view/dataset/600028"}], "date_created": "Sun, 01 Feb 2009 00:00:00 GMT", "description": "#0125098\u003cbr/\u003eSteve Emslie\u003cbr/\u003e\u003cbr/\u003eOccupation History and Diet of Adelie Penguins in the Ross Sea Region\u003cbr/\u003e\u003cbr/\u003eThis project will build on previous studies to investigate the occupation history and diet of Adelie penguins (Pygoscelis adeliae) in the Ross Sea region, Antarctica, with excavations of abandoned and active penguin colonies. Numerous active and abandoned colonies exist on the Victoria Land coast, from Cape Adare to Marble Point will be sampled. Some of these sites have been radiocarbon-dated and indicate a long occupation history for Adelie penguins extending to 13,000 years before present (B. P.). The material recovered from excavations, as demonstrated from previous investigations, will include penguin bones, tissue, and eggshell fragments as well as abundant remains of prey (fish bones, otoliths, squid beaks) preserved in ornithogenic (formed from bird guano) soils. These organic remains will be quantified and subjected to radiocarbon analyses to obtain a colonization history of penguins in this region. Identification of prey remains in the sediments will allow assessment of penguin diet. Other data (ancient DNA) from these sites will be analyzed through collaboration with New Zealand scientists. Past climatic conditions will be interpreted from published ice-core and marine-sediment records. These data will be used to test the hypothesis that Adelie penguins respond to climate change, past and present, in a predictable manner. In addition, the hypothesis that Adelie penguins alter their diet in accordance with climate, sea-ice conditions, and other marine environmental variables along a latitudinal gradient will be tested. Graduate and undergraduate students will be involved in this project and a project Web site will be developed to report results and maintain educational interaction between the PI and students at local middle and high schools in Wilmington, NC.", "east": 160.0, "geometry": "POINT(55 -75)", "instruments": null, "is_usap_dc": true, "keywords": "FIELD SURVEYS", "locations": null, "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Emslie, Steven", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -90.0, "title": "Occupation History and Diet of Adelie Penguins in the Ross Sea Region", "uid": "p0000220", "west": -50.0}, {"awards": "0536870 Rogers, Scott", "bounds_geometry": "POINT(-106.8 -72.4667)", "dataset_titles": "Comprehensive Biological Study of Vostok Accretion Ice", "datasets": [{"dataset_uid": "600052", "doi": "10.15784/600052", "keywords": "Antarctica; Biology; Biosphere; Cryosphere; Glaciers/Ice Sheet; Glaciology; Hydrothermal Vent; Lake Vostok; Microbes; Subglacial lakes", "people": "Rogers, Scott O.", "repository": "USAP-DC", "science_program": null, "title": "Comprehensive Biological Study of Vostok Accretion Ice", "url": "https://www.usap-dc.org/view/dataset/600052"}], "date_created": "Tue, 02 Sep 2008 00:00:00 GMT", "description": "The large subglacial Lake Vostok in Antarctica is unique ecological site with a novel microbial biota. The temperatures, pressures and lack of light all select for organisms that may not exist anywhere else on Earth. The accretion ice (lake water frozen to the bottom of the lower surface of the glacier) has preserved microbial samples from each region of Lake Vostok as the glacier passes over and into the lake. Thus, without contaminating the lake with microorganisms from the surface, microbes originating from the lake can be collected, transported to the laboratory and studied. Two of the deepest ice cores sections in this project are part of the international allocation. The will be shared between four researchers (Sergey Bulat from Russia, Jean-Robert Petit and Daniel Prieur from France, Scott Rogers from USA). The United States team will study, isolate, and characterize bacteria, fungi, and viruses that have been sampled from the lake through the process of ice accretion to the lower surface of 3500+m thick glacier overriding the lake. The project will involve a suite of methods, including molecular, morphological, and cultural. This includes observation and description by fluorescence, light, and electron microscopy, isolation on thirteen separate cultural media, polymerase chain reaction amplification, DNA sequencing, and phylogenetic analyses. Eleven accretion ice core sections, as well as two glacial ice core sections. As well as two glacial ice core sections will be studied. The accretion ice core sections, as well as two glacial ice core sections will be studied. The accretion ice core sections represent all of the major regions of the lake that have been sampled by the accretion process in the vicinity of the Vostok 5G ice core. The broader impacts of the work relate to the impact the results will have on the filed. These long=isolated lakes, deep below the Antarctic ice sheet may contain novel uniquely adapted organisms. Glacial ice contains an enormous diversity of entrapped microbes, some of which may be metabolically active in the ice. The microbes from Lake Vostok are of special interest, since they are adapted to cold, dark, and high pressure. Thus, their enzyme systems and biochemical pathways may be significantly different from those in the microbes that are the subject of current studies. As such, these organisms may form compounds that may have useful applications. Also, study of the accretion ice, and eventually the water, from Lake Vostok will provide a basis for the study of other subglacial lakes. Additionally, study of the microbes in the accretion ice will be useful to those planning to study analogous systems on ice-covered planets and moons.", "east": -106.8, "geometry": "POINT(-106.8 -72.4667)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -72.4667, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Rogers, Scott O.", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -72.4667, "title": "Comprehensive Biological Study of Vostok Accretion Ice", "uid": "p0000566", "west": -106.8}, {"awards": "0127022 Jeffrey, Wade", "bounds_geometry": "POLYGON((-177.639 -43.5676,-143.1091 -43.5676,-108.5792 -43.5676,-74.0493 -43.5676,-39.5194 -43.5676,-4.9895 -43.5676,29.5404 -43.5676,64.0703 -43.5676,98.6002 -43.5676,133.1301 -43.5676,167.66 -43.5676,167.66 -46.99877,167.66 -50.42994,167.66 -53.86111,167.66 -57.29228,167.66 -60.72345,167.66 -64.15462,167.66 -67.58579,167.66 -71.01696,167.66 -74.44813,167.66 -77.8793,133.1301 -77.8793,98.6002 -77.8793,64.0703 -77.8793,29.5404 -77.8793,-4.9895 -77.8793,-39.5194 -77.8793,-74.0493 -77.8793,-108.5792 -77.8793,-143.1091 -77.8793,-177.639 -77.8793,-177.639 -74.44813,-177.639 -71.01696,-177.639 -67.58579,-177.639 -64.15462,-177.639 -60.72345,-177.639 -57.29228,-177.639 -53.86111,-177.639 -50.42994,-177.639 -46.99877,-177.639 -43.5676))", "dataset_titles": "Expedition Data; Ross Sea microbial biomass and production", "datasets": [{"dataset_uid": "001584", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/NBP0508"}, {"dataset_uid": "001690", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/NBP0304B"}, {"dataset_uid": "600029", "doi": "10.15784/600029", "keywords": "Biology; Biosphere; Chemistry:Fluid; CTD Data; Microbiology; Oceans; Phytoplankton; Ross Sea; Southern Ocean", "people": "Jeffrey, Wade H.", "repository": "USAP-DC", "science_program": null, "title": "Ross Sea microbial biomass and production", "url": "https://www.usap-dc.org/view/dataset/600029"}], "date_created": "Thu, 12 Jun 2008 00:00:00 GMT", "description": "Ultraviolet radiation influences the dynamics of plankton processes in the near-surface waters of most aquatic ecosystems. In particular, the Southern Ocean is affected in the austral spring period when biologically damaging ultraviolet radiation is enhanced by ozone depletion. While progress has been made in estimating the quantitative impact of ultraviolet radiation on bacteria and phytoplankton in the Southern Ocean, some important issues remain to be resolved. Little is known about responses in systems dominated by the colonial haptophyte Phaeocystis antarctica, which dominates spring blooms in a polyna that develops in the southern Ross Sea. The Ross Sea is also of interest because of the occurrence of open water at a far southerly location in the spring, well within the ozone hole, and continuous daylight, with implications for the regulation of DNA repair. A number of studies suggest that vertical mixing can significant modify the impact of ultraviolet radiation in the Southern Ocean and elsewhere. However, there are limited measurements of turbulence intensity in the surface layer and measurements have not been integrated with parallel studies of ultraviolet radiation effects on phytoplankton and bacterioplankton. To address these issues, this collaborative study will focus on vertical mixing and the impact of ultraviolet radiation in the Ross Sea. The spectral and temporal responses of phytoplankton and bacterioplankton to ultraviolet radiation will be characterized in both laboratory and solar incubations. These will lead to the definition of biological weighting functions and response models capable of predicting the depth and time distribution of ultraviolet radiation impacts on photosynthesis, bacterial incorporation and DNA damage in the surface layer. Diel sampling will measure depth-dependent profiles of DNA damage, bacterial incorporation, photosynthesis and fluorescence parameters over a 24 h cycle. Sampling will include stations with contrasting wind-driven mixing and stratification as the polyna develops. The program of vertical mixing measurements is optimized for the typical springtime Ross Sea situation in which turbulence of intermediate intensity is insufficient to mix the upper layer thoroughly in the presence of stabilizing influences like solar heating and/or surface freshwater input from melting ice. Fine-scale vertical density profiles will be measured with a free-fall CTD unit and the profiles will be used to directly estimate large-eddy scales by determining Thorpe scales. Eddy scales and estimated turbulent diffusivities will be directly related to surface layer effects, and used to generate lagrangian depth-time trajectories in models of ultraviolet radiation responses in the surface mixed layer. The proposed research will be the first in-depth study of ultraviolet radiation effects in the Ross Sea and provide a valuable comparison with previous work in the Weddell-Scotia Confluence and Palmer Station regions. It will also enhance the understanding of vertical mixing processes, trophic interactions and biogeochemical cycling in the Ross Sea.", "east": 167.66, "geometry": "POINT(-4.9895 -60.72345)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; IN SITU/LABORATORY INSTRUMENTS \u003e MAGNETIC/MOTION SENSORS \u003e GRAVIMETERS \u003e GRAVIMETERS; IN SITU/LABORATORY INSTRUMENTS \u003e PRESSURE/HEIGHT METERS \u003e PRESSURE SENSORS; IN SITU/LABORATORY INSTRUMENTS \u003e PHOTON/OPTICAL DETECTORS \u003e TURBIDITY METERS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ADCP; IN SITU/LABORATORY INSTRUMENTS \u003e SAMPLERS \u003e BOTTLES/FLASKS/JARS \u003e WATER BOTTLES; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e MSBS; IN SITU/LABORATORY INSTRUMENTS \u003e CHEMICAL METERS/ANALYZERS \u003e FLUORESCENCE MICROSCOPY; IN SITU/LABORATORY INSTRUMENTS \u003e PHOTON/OPTICAL DETECTORS \u003e MICROSCOPES; IN SITU/LABORATORY INSTRUMENTS \u003e CHEMICAL METERS/ANALYZERS \u003e FLUOROMETERS; IN SITU/LABORATORY INSTRUMENTS \u003e SAMPLERS \u003e BOTTLES/FLASKS/JARS \u003e GO-FLO BOTTLES", "is_usap_dc": true, "keywords": "R/V NBP; B-15J", "locations": null, "north": -43.5676, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Jeffrey, Wade H.; Neale, Patrick", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repo": "R2R", "repositories": "Other; USAP-DC", "science_programs": null, "south": -77.8793, "title": "Collaborative Proposal: Interactive Effects of UV Radiation and Vertical Mixing on Phytoplankton and Bacterial Productivity of Ross See Phaeocystis Blooms", "uid": "p0000578", "west": -177.639}]

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