USAP-DC

{"dp_type": "Project", "free_text": "Blood"}

[{"awards": "1643575 Kanatous, Shane; 1644256 Costa, Daniel; 1644004 Trumble, Stephen", "bounds_geometry": "POLYGON((-66.534369 -52.962091,-65.3857434 -52.962091,-64.2371178 -52.962091,-63.0884922 -52.962091,-61.9398666 -52.962091,-60.791241 -52.962091,-59.6426154 -52.962091,-58.4939898 -52.962091,-57.3453642 -52.962091,-56.1967386 -52.962091,-55.048113 -52.962091,-55.048113 -54.530129,-55.048113 -56.098167000000004,-55.048113 -57.666205000000005,-55.048113 -59.234243,-55.048113 -60.802281,-55.048113 -62.370319,-55.048113 -63.938357,-55.048113 -65.506395,-55.048113 -67.074433,-55.048113 -68.642471,-56.1967386 -68.642471,-57.3453642 -68.642471,-58.4939898 -68.642471,-59.6426154 -68.642471,-60.791241 -68.642471,-61.9398666 -68.642471,-63.0884922 -68.642471,-64.2371178 -68.642471,-65.3857434 -68.642471,-66.534369 -68.642471,-66.534369 -67.074433,-66.534369 -65.506395,-66.534369 -63.938356999999996,-66.534369 -62.370319,-66.534369 -60.802281,-66.534369 -59.234243,-66.534369 -57.666205,-66.534369 -56.098167000000004,-66.534369 -54.530129,-66.534369 -52.962091))", "dataset_titles": "Data from: Whiskers provide time-series of toxic and essential trace elements, Se:Hg molar ratios, and stable isotope values of an apex Antarctic predator, the leopard seal; Leopard Seal Diving behavior data; Leopard Seal movement data", "datasets": [{"dataset_uid": "601689", "doi": "10.15784/601689", "keywords": "Antarctica; Antarctic Peninsula; Biota; Body Mass; Diving Behavior; Leopard Seal; Movement Data; Seals", "people": "Costa, Daniel", "repository": "USAP-DC", "science_program": null, "title": "Leopard Seal movement data", "url": "https://www.usap-dc.org/view/dataset/601689"}, {"dataset_uid": "200361", "doi": "https://doi.org/10.5061/dryad.ksn02v75b", "keywords": null, "people": null, "repository": "Dryad", "science_program": null, "title": "Data from: Whiskers provide time-series of toxic and essential trace elements, Se:Hg molar ratios, and stable isotope values of an apex Antarctic predator, the leopard seal", "url": "https://datadryad.org/stash/dataset/doi:10.5061%2Fdryad.ksn02v75b"}, {"dataset_uid": "601690", "doi": "10.15784/601690", "keywords": "Antarctica; Antarctic Peninsula; Biota; Body Mass; Diving Behavior; Leopard Seal; Seals", "people": "Costa, Daniel", "repository": "USAP-DC", "science_program": null, "title": "Leopard Seal Diving behavior data", "url": "https://www.usap-dc.org/view/dataset/601690"}], "date_created": "Fri, 12 May 2023 00:00:00 GMT", "description": "This research project is a multidisciplinary effort that brings together a diverse team of scientists from multiple institutions together to understand the foraging behavior and physiology of leopard seals and their role in the Southern Ocean food web. The project will examine the physiology and behavior of leopard seals to in an effort to determine their ability to respond to potential changes in their habitat and foraging areas. Using satellite tracking devices the team will examine the movement and diving behavior of leopard seals and couple this information with measurements of their physiological capacity. The project will determine whether leopard seals- who feed on diverse range of prey- are built differently than their deep diving relatives the Weddell and elephant seal who feed on fish and squid. The team will also determine whether leopard seals are operating at or near their physiological capability to determine how much, if any, ?reserve capacity? they might have to forage and live in changing environments. A better understanding of their home ranges, movement patterns, and general behavior will also be informative to help in managing human-leopard seal interactions. The highly visual nature of the data and analysis for this project lends itself to public and educational display and outreach, particularly as they relate to the changing Antarctic habitats. The project will use the research results to educate the public on the unique physiological and ecological adaptations to extreme environments seen in diving marine mammals, including adaptations to exercise under low oxygen conditions and energy utilization, which affect and dictate the lifestyle of these exceptional organisms. The results of the project will also contribute to the broader understanding that may enhance the aims of managing marine living resources. The leopard seal is an apex predator in the Antarctic ecosystem. This project seeks to better understand the ability of the leopard seal to cope with a changing environment. The project will first examine the foraging behavior and habitat utilization of leopard seals using satellite telemetry. Specifically, satellite telemetry tags will be used to obtain dive profiles and movement data for individuals across multiple years. Diet and trophic level positions across multiple temporal scales will then be determined from physiological samples (e.g., blood, vibrissae, blubber fatty acids, stable isotopes, fecal matter). Oceanographic data will be integrated with these measures to develop habitat models that will be used to assess habitat type, habitat utilization, habitat preference, and home range areas for individual animals. Diet composition for individual seals will be evaluated to determine whether specific animals are generalists or specialists. Second, the team will investigate the physiological adaptations that allow leopard seals to be apex predators and determine to what extent leopard seals are working at or near their physiological limit. Diving behavior and physiology of leopard seals will be evaluated (for instance the aerobic dive limit for individual animals and skeletal muscle adaptations will be determined for diving under hypoxic conditions). Data from time-depth recorders will be used to determine foraging strategies for individual seals, and these diving characteristics will be related to physiological variables (e.g., blood volume, muscle oxygen stores) to better understand the link between foraging behavior and physiology. The team will compare myoglobin storage in swimming muscles associated with both forelimb and hind limb propulsion and the use of anaerobic versus aerobic metabolic systems while foraging.", "east": -55.048113, "geometry": "POINT(-60.791241 -60.802281)", "instruments": null, "is_usap_dc": true, "keywords": "Antarctic Peninsula; Diving Behavior; MAMMALS; MARINE ECOSYSTEMS; Movement Patterns; Leopard Seal", "locations": "Antarctic Peninsula", "north": -52.962091, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Costa, Daniel; Trumble, Stephen J; Kanatous, Shane", "platforms": null, "repo": "USAP-DC", "repositories": "Dryad; USAP-DC", "science_programs": null, "south": -68.642471, "title": "Collaborative Research: Foraging Ecology and Physiology of the Leopard Seal", "uid": "p0010419", "west": -66.534369}, {"awards": "1543383 Postlethwait, John; 1947040 Postlethwait, John; 2232891 Postlethwait, John", "bounds_geometry": "POLYGON((-180 -37,-144 -37,-108 -37,-72 -37,-36 -37,0 -37,36 -37,72 -37,108 -37,144 -37,180 -37,180 -42.3,180 -47.6,180 -52.9,180 -58.2,180 -63.5,180 -68.8,180 -74.1,180 -79.4,180 -84.69999999999999,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -84.7,-180 -79.4,-180 -74.1,-180 -68.8,-180 -63.5,-180 -58.2,-180 -52.9,-180 -47.6,-180 -42.300000000000004,-180 -37))", "dataset_titles": "aBSREL tests for episodic diversifying selection on hemoglobin genes in notothenioids.; MEME tests of sites evolving under episodic diversifying selection in notothenioid hemoglobin genes.; Notothenioid hemoglobin protein 3D modeling.; Notothenioid species tree used in the study.; Phylogenetic trees of hemoglobin proteins in notothenioids.; Rates of hemoglobin evolution among genes and across notothenioid species.; RELAX tests for pervasive changes in strength of natural selection on hemoglobin genes in notothenioids.", "datasets": [{"dataset_uid": "601732", "doi": "10.15784/601732", "keywords": "Antarctica; Cold Adaptation; Cryonotothenioid; Dragonfish; Eleginopsioidea; Fish; Genetic Analysis; Hemoglobin; Icefish; Notothenioid; Notothenioid Fishes; Plunderfish; Sub-Antarctic", "people": "Postlethwait, John; Desvignes, Thomas", "repository": "USAP-DC", "science_program": null, "title": "Notothenioid hemoglobin protein 3D modeling.", "url": "https://www.usap-dc.org/view/dataset/601732"}, {"dataset_uid": "601721", "doi": "10.15784/601721", "keywords": "Antarctica; Cold Adaptation; Cryonotothenioid; Dragonfish; Eleginopsioidea; Fish; Gene; Hemoglobin; Icefish; Notothenioid; Plunderfish; Sub-Antarctic", "people": "Desvignes, Thomas; Postlethwait, John", "repository": "USAP-DC", "science_program": null, "title": "Notothenioid species tree used in the study.", "url": "https://www.usap-dc.org/view/dataset/601721"}, {"dataset_uid": "601722", "doi": "10.15784/601722", "keywords": "Antarctica; Cold Adaptation; Cryonotothenioid; Dragonfish; Eleginopsioidea; Fish; Gene; Hemoglobin; Icefish; Notothenioid; Plunderfish; Sub-Antarctic", "people": "Postlethwait, John; Desvignes, Thomas", "repository": "USAP-DC", "science_program": null, "title": "Phylogenetic trees of hemoglobin proteins in notothenioids.", "url": "https://www.usap-dc.org/view/dataset/601722"}, {"dataset_uid": "601728", "doi": "10.15784/601728", "keywords": "Antarctica; Cold Adaptation; Cryonotothenioid; Dragonfish; Eleginopsioidea; Fish; Gene; Genetic Analysis; Hemoglobin; Icefish; Notothenioid; Notothenioid Fishes; Plunderfish; Sub-Antarctic", "people": "Desvignes, Thomas; Postlethwait, John", "repository": "USAP-DC", "science_program": null, "title": "aBSREL tests for episodic diversifying selection on hemoglobin genes in notothenioids.", "url": "https://www.usap-dc.org/view/dataset/601728"}, {"dataset_uid": "601729", "doi": "10.15784/601729", "keywords": "Antarctica; Cold Adaptation; Cryonotothenioid; Dragonfish; Eleginopsioidea; Fish; Genetic Analysis; Hemoglobin; Icefish; Notothenioid; Notothenioid Fishes; Plunderfish; Sub-Antarctic", "people": "Desvignes, Thomas; Postlethwait, John", "repository": "USAP-DC", "science_program": null, "title": "Rates of hemoglobin evolution among genes and across notothenioid species.", "url": "https://www.usap-dc.org/view/dataset/601729"}, {"dataset_uid": "601730", "doi": "10.15784/601730", "keywords": "Antarctica; Cold Adaptation; Cryonotothenioid; Dragonfish; Eleginopsioidea; Fish; Genetic Analysis; Hemoglobin; Icefish; Notothenioid; Notothenioid Fishes; Plunderfish; Sub-Antarctic", "people": "Postlethwait, John; Desvignes, Thomas", "repository": "USAP-DC", "science_program": null, "title": "MEME tests of sites evolving under episodic diversifying selection in notothenioid hemoglobin genes.", "url": "https://www.usap-dc.org/view/dataset/601730"}, {"dataset_uid": "601731", "doi": "10.15784/601731", "keywords": "Antarctica; Cold Adaptation; Cryonotothenioid; Dragonfish; Eleginopsioidea; Fish; Genetic Analysis; Hemoglobin; Icefish; Notothenioid; Notothenioid Fishes; Plunderfish; Sub-Antarctic", "people": "Postlethwait, John; Desvignes, Thomas", "repository": "USAP-DC", "science_program": null, "title": "RELAX tests for pervasive changes in strength of natural selection on hemoglobin genes in notothenioids.", "url": "https://www.usap-dc.org/view/dataset/601731"}], "date_created": "Wed, 03 May 2023 00:00:00 GMT", "description": "Antarctic notothenioid fishes, also known as cryonotothenioids, inhabit the icy and highly oxygenated waters surrounding the Antarctic continent after diverging from notothenioids inhabiting more temperate waters. Notothenioid hemoglobin and blood parameters are known to have evolved along with the establishment of stable polar conditions, and among Antarctic notothenioids, icefishes are evolutionary oddities living without hemoglobin following the deletion of all functional hemoglobin genes from their genomes. In this project, we investigate the evolution of hemoglobin genes and gene clusters across the notothenioid radiation until their loss in the icefish ancestor after its divergence from the dragonfish lineage to understand the forces, mechanisms, and potential causes for hemoglobin gene loss in the icefish ancestor.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "FISH; Icefish; Cryonotothenioid; Gene; Plunderfish; Eleginopsioidea; AQUATIC ECOSYSTEMS; Dragonfish; Sub-Antarctic; Notothenioid; Blood; Hemoglobin", "locations": "Sub-Antarctic", "north": -37.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Desvignes, Thomas; Postlethwait, John", "platforms": null, "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -90.0, "title": "Evolution of hemoglobin genes in notothenioid fishes", "uid": "p0010417", "west": -180.0}, {"awards": "1644004 Trumble, Stephen", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "Data from: Whiskers provide time-series of toxic and essential trace elements, Se:Hg molar ratios, and stable isotope values of an apex Antarctic predator, the leopard seal", "datasets": [{"dataset_uid": "200338", "doi": "doi:10.5061/dryad.ksn02v75b", "keywords": null, "people": null, "repository": "Dryad", "science_program": null, "title": "Data from: Whiskers provide time-series of toxic and essential trace elements, Se:Hg molar ratios, and stable isotope values of an apex Antarctic predator, the leopard seal", "url": "https://datadryad.org/stash/share/h6UwXvfhZG26jtPTtDqyXNMnx2UWknOqmv05EBz6A10"}], "date_created": "Tue, 06 Dec 2022 00:00:00 GMT", "description": "This research project is a multidisciplinary effort that brings together a diverse team of scientists from multiple institutions together to understand the foraging behavior and physiology of leopard seals and their role in the Southern Ocean food web. The project will examine the physiology and behavior of leopard seals to in an effort to determine their ability to respond to potential changes in their habitat and foraging areas. Using satellite tracking devices the team will examine the movement and diving behavior of leopard seals and couple this information with measurements of their physiological capacity. The project will determine whether leopard seals- who feed on diverse range of prey- are built differently than their deep diving relatives the Weddell and elephant seal who feed on fish and squid. The team will also determine whether leopard seals are operating at or near their physiological capability to determine how much, if any, ?reserve capacity? they might have to forage and live in changing environments. A better understanding of their home ranges, movement patterns, and general behavior will also be informative to help in managing human-leopard seal interactions. The highly visual nature of the data and analysis for this project lends itself to public and educational display and outreach, particularly as they relate to the changing Antarctic habitats. The project will use the research results to educate the public on the unique physiological and ecological adaptations to extreme environments seen in diving marine mammals, including adaptations to exercise under low oxygen conditions and energy utilization, which affect and dictate the lifestyle of these exceptional organisms. The results of the project will also contribute to the broader understanding that may enhance the aims of managing marine living resources. The leopard seal is an apex predator in the Antarctic ecosystem. This project seeks to better understand the ability of the leopard seal to cope with a changing environment. The project will first examine the foraging behavior and habitat utilization of leopard seals using satellite telemetry. Specifically, satellite telemetry tags will be used to obtain dive profiles and movement data for individuals across multiple years. Diet and trophic level positions across multiple temporal scales will then be determined from physiological samples (e.g., blood, vibrissae, blubber fatty acids, stable isotopes, fecal matter). Oceanographic data will be integrated with these measures to develop habitat models that will be used to assess habitat type, habitat utilization, habitat preference, and home range areas for individual animals. Diet composition for individual seals will be evaluated to determine whether specific animals are generalists or specialists. Second, the team will investigate the physiological adaptations that allow leopard seals to be apex predators and determine to what extent leopard seals are working at or near their physiological limit. Diving behavior and physiology of leopard seals will be evaluated (for instance the aerobic dive limit for individual animals and skeletal muscle adaptations will be determined for diving under hypoxic conditions). Data from time-depth recorders will be used to determine foraging strategies for individual seals, and these diving characteristics will be related to physiological variables (e.g., blood volume, muscle oxygen stores) to better understand the link between foraging behavior and physiology. The team will compare myoglobin storage in swimming muscles associated with both forelimb and hind limb propulsion and the use of anaerobic versus aerobic metabolic systems while foraging.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "MAMMALS; Stable Isotopes; Livingston Island", "locations": "Livingston Island", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Trumble, Stephen J", "platforms": null, "repo": "Dryad", "repositories": "Dryad", "science_programs": null, "south": -90.0, "title": "Collaborative Research: Foraging Ecology and Physiology of the Leopard Seal", "uid": "p0010394", "west": -180.0}, {"awards": "1645087 Catchen, Julian", "bounds_geometry": null, "dataset_titles": "Chromosome-Level Genome Assembly and Circadian Gene Repertoire of the Patagonia Blennie Eleginops maclovinus\u2014The Closest Ancestral Proxy of Antarctic Cryonotothenioids; Evaluating Illumina-, Nanopore-, and PacBio-based genome assembly strategies with the bald notothen, Trematomus borchgrevinki; Genomics of Secondarily Temperate Adaptation in the Only Non-Antarctic Icefish", "datasets": [{"dataset_uid": "200331", "doi": "10.5061/dryad.ghx3ffbs3", "keywords": null, "people": null, "repository": "Dryad", "science_program": null, "title": "Evaluating Illumina-, Nanopore-, and PacBio-based genome assembly strategies with the bald notothen, Trematomus borchgrevinki", "url": "https://datadryad.org/stash/dataset/doi:10.5061/dryad.ghx3ffbs3"}, {"dataset_uid": "200381", "doi": "", "keywords": null, "people": null, "repository": "NCBI", "science_program": null, "title": "Chromosome-Level Genome Assembly and Circadian Gene Repertoire of the Patagonia Blennie Eleginops maclovinus\u2014The Closest Ancestral Proxy of Antarctic Cryonotothenioids", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA917608"}, {"dataset_uid": "200330", "doi": "", "keywords": null, "people": null, "repository": "NCBI ", "science_program": null, "title": "Evaluating Illumina-, Nanopore-, and PacBio-based genome assembly strategies with the bald notothen, Trematomus borchgrevinki", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA861284"}, {"dataset_uid": "200380", "doi": "", "keywords": null, "people": null, "repository": "NCBI", "science_program": null, "title": "Genomics of Secondarily Temperate Adaptation in the Only Non-Antarctic Icefish", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA857989"}], "date_created": "Mon, 10 Oct 2022 00:00:00 GMT", "description": "As plate tectonics pushed Antarctica into a polar position, by ~34 million years ago, the continent and its surrounding Southern Ocean (SO) became geographically and thermally isolated by the Antarctic Circumpolar Current. Terrestrial and marine glaciation followed, resulting in extinctions as well as the survival and radiation of unique flora and fauna. The notothenioid fish survived and arose from a common ancestral stock into tax with 120 species that dominates today?s SO fish fauna. The Notothenioids evolved adaptive traits including novel antifreeze proteins for survival in extreme cold, but also suffered seemingly adverse trait loss including red blood cells in the icefish family, and the ability to mount cellular responses to mitigate heat stress ? otherwise ubiquitous across all life. This project aims to understand how the notothenoid genomes have changed and contributed to their evolution in the cold. The project will sequence, analyze and compare the genomes of two strategic pairs of notothenioid fishes representing both red-blooded and white-blooded species. Each pair will consist of one Antarctic species and one that has readapted to the temperate waters of S. America or New Zealand. The project will also compare the Antarctic species genomes to a genome of the closet non-Antarctic relative representing the temperate notothenioid ancestor. The work aims to uncover the mechanisms that enabled the adaptive evolution of this ecologically vital group of fish in the freezing Southern Ocean, and shed light on their adaptability to a warming world. The finished genomes will be made available to promote and advance Antarctic research and the project will host a symposium of Polar researchers to discuss the cutting edge developments regarding of genomic adaptations in the polar region. Despite subzero, icy conditions that are perilous to teleost fish, the fish fauna of the isolated Southern Ocean (SO) surrounding Antarctica is remarkably bountiful. A single teleost group ? the notothenioid fishes ? dominate the fauna, comprising over 120 species that arose from a common ancestor. When Antarctica became isolated and SO temperatures began to plunge in early Oligocene, the prior temperate fishes became extinct. The ancestor of Antarctic notothenioids overcame forbidding polar conditions and, absent niche competition, it diversified and filled the SO. How did notothenioids adapt to freezing environmental selection pressures and achieve such extraordinary success? And having specialized to life in chronic cold for 30 myr, can they evolve in pace with today?s warming climate to stay viable? Past studies of Antarctic notothenioid evolutionary adaptation have discovered various remarkable traits including the key, life-saving antifreeze proteins. But life specialized to cold also led to paradoxical trait changes such as the loss of the otherwise universal heat shock response, and of the O2-transporting hemoglobin and red blood cells in the icefish family. A few species interestingly regained abilities to live in temperate waters following the escape of their ancestor out of the freezing SO. This proposed project is the first major effort to advance the field from single trait studies to understanding the full spectrum of genomic and genetic responses to climatic and environmental change during notothenioid evolution, and to evaluate their adaptability to continuing climate change. To this end, the project will sequence the genomes of four key species that embody genomic responses to different thermal selection regimes during notothenioids? evolutionary history, and by comparative analyses of genomic structure, architecture and content, deduce the responding changes. Specifically, the project will (i) obtain whole genome assemblies of the red-blooded T. borchgrevinki and the S. American icefish C. esox; (ii) using the finished genomes from (i) as template, obtain assemblies of the New Zealand notothenioid N. angustata, and the white-blooded icefish C. gunnari, representing a long (11 myr) and recent (1 myr) secondarily temperate evolutionary history respectively. Genes that are under selection in the temperate environment but not in the Antarctic environment can be inferred to be directly necessary for that environment ? and the reverse is also true for genes under selection in the Antarctic but not in the temperate environment. Further, genes important for survival in temperate waters will show parallel selection between N. angustata and C. esox despite the fact that the two fish left the Antarctic at far separated time points. Finally, gene families that expanded due to strong selection within the cold Antarctic should show a degradation of duplicates in the temperate environment. The project will test these hypotheses using a number of techniques to compare the content and form of genes, the structure of the chromosomes containing those genes, and through the identification of key characters, such as selfish genetic elements, introns, and structural variants.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "Genome Assembly; FISH; McMurdo Sound; Icefish; SHIPS; Notothenioid; Puerto Natales, Chile", "locations": "McMurdo Sound; Puerto Natales, Chile", "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Catchen, Julian; Cheng, Chi-Hing", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e SHIPS", "repo": "Dryad", "repositories": "Dryad; NCBI; NCBI ", "science_programs": null, "south": null, "title": "Evolutionary Genomic Responses in Antarctic Notothenioid Fishes", "uid": "p0010384", "west": null}, {"awards": "1443637 Zakon, Harold", "bounds_geometry": null, "dataset_titles": "Evolutionary analysis of transient receptor potential (TRP) channels in notothenioid fishes; Functional characterization of temperature activated ion channels from Antarctic fishes; TagSeq tissue specific expression data for Antarctic Harpagifer antarcticus and tropical African cichlid Astatotilapia (Haplochromis) burtoni", "datasets": [{"dataset_uid": "200292", "doi": "10.18738/T8/NXGNEI", "keywords": null, "people": null, "repository": "Texas Data Repository", "science_program": null, "title": "Evolutionary analysis of transient receptor potential (TRP) channels in notothenioid fishes", "url": "https://doi.org/10.18738/T8/NXGNEI"}, {"dataset_uid": "601695", "doi": "10.15784/601695", "keywords": "Antarctica; Notothenioid; Southern Ocean", "people": "York, Julia", "repository": "USAP-DC", "science_program": null, "title": "Functional characterization of temperature activated ion channels from Antarctic fishes", "url": "https://www.usap-dc.org/view/dataset/601695"}, {"dataset_uid": "200293", "doi": "", "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "TagSeq tissue specific expression data for Antarctic Harpagifer antarcticus and tropical African cichlid Astatotilapia (Haplochromis) burtoni", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA758918"}], "date_created": "Fri, 03 Jun 2022 00:00:00 GMT", "description": "This project studies how the proteins of the nerves and muscles of fish that live in Antarctica function in the cold, which should provide information on the function of these same proteins in all animals, including humans. These proteins, called ion channels, open and close to allow ions (atoms or molecules with electrical charge) to flow into or out of cells which causes the electrical activity of nerves and muscles. Mutations that influence this process are the basis of numerous human disorders such as epilepsy, heart arrhythmias, and muscle paralysis. Thus, it is important to understand what parts of the proteins govern these transitions. The speed with which channels open and close depends on temperature. Human ion channels transition slowly when we are cold, which is why we become numb in the cold. Yet Antarctic fish, called icefish, are active at freezing temperatures that drastically limit the activity of human ion channels. The investigators have evidence that specific alterations in the icefishs\u0027 ion channels allow their channels to operate differently in the cold and they will use gene discovery and biophysical methods to test how these changes alter the transitions of icefish proteins at different temperatures. The project will also further the NSF goals of training new generations of scientists and of making scientific discoveries available to the general public. The gene discovery analysis will be done by undergraduate students including those from a minority-serving university and the investigators will develop a new course which will also serve students at that institution and elsewhere. In addition, the investigators will participate in educational outreach events with the general public as well as with groups with special needs. Notothenioid fishes are one of the most successful groups of vertebrates in Antarctica. Notothens have adaptations to the freezing water they inhabit and this project will study how their voltage-gated ion channels (VGICs) function in the cold. The molecular movements of ion channels are severely impaired by cold, yet notothens function at temperatures that would paralyze the nerves and muscles of \"cold-blooded\" temperate zone animals. Surprisingly, no biophysical or molecular investigations have been conducted on notothen VGICs. The investigators have preliminary data that amino acid substitutions occur at sites in VGICs that are evolutionarily conserved from fruit flies to humans. Some of these sites are known to impact channel function and the role of others in channel transitioning are unknown. The results from studying them will provide novel information also applicable to non-notothen, perhaps even human, VGICs as well as providing insights into how VGICs adapt to the cold. The project will biophysically characterize notothen VGICs using voltage-clamp techniques will and compare their properties over a range of temperatures to the same channel from two temperate zone fish. The role of unique notothen amino acid substitutions will be characterized by mutagenesis. One specific aim will be a project in which undergraduates mine notothen sequence databases to identify other potential amino acid substitutions in VGICs that might facilitate adaptation to the cold.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "Amd/Us; USAP-DC; USA/NSF; FIELD INVESTIGATION; AMD; FISHERIES", "locations": null, "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Zakon, Harold", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION", "repo": "Texas Data Repository", "repositories": "GenBank; Texas Data Repository; USAP-DC", "science_programs": null, "south": null, "title": "Analysis of Voltage-gated Ion Channels in Antarctic Fish", "uid": "p0010331", "west": null}, {"awards": "2020664 Vazquez-Medina, Jose Pablo; 2020706 Hindle, Allyson", "bounds_geometry": "POLYGON((164 -77.2,164.3 -77.2,164.6 -77.2,164.9 -77.2,165.2 -77.2,165.5 -77.2,165.8 -77.2,166.1 -77.2,166.4 -77.2,166.7 -77.2,167 -77.2,167 -77.265,167 -77.33,167 -77.395,167 -77.46,167 -77.525,167 -77.59,167 -77.655,167 -77.72,167 -77.785,167 -77.85,166.7 -77.85,166.4 -77.85,166.1 -77.85,165.8 -77.85,165.5 -77.85,165.2 -77.85,164.9 -77.85,164.6 -77.85,164.3 -77.85,164 -77.85,164 -77.785,164 -77.72,164 -77.655,164 -77.59,164 -77.525,164 -77.46,164 -77.395,164 -77.33,164 -77.265,164 -77.2))", "dataset_titles": null, "datasets": null, "date_created": "Thu, 09 Sep 2021 00:00:00 GMT", "description": "This award is funded in whole or in part under the American Rescue Plan Act of 2021 (Public Law 117-2). Part I: Non-technical description: The Weddell seal is an iconic Antarctic species and a superb diver, swimming down to 2,000 feet and staying underwater for up to 45 minutes. However, as for any mammal, the low oxygen concentrations in the blood during diving and the recovery once back at the surface are challenges that need to be overcome making their diving ability something unique that has fascinated scientists for decades. This research project will evaluate the underlying processes in Weddell seal\u2019s physiology that protects this species from the consequences of diving. The work will combine laboratory experiments where cells that line the blood vessels will be exposed to conditions of low oxygen, similar to those that will be measured in diving seals in Antarctica. The investigarors will test a new idea that several short-term dives, performed before a long dive, allows seals to condition themselves. Measurements on the chemical compounds released to the blood during dives, combined with experiments on the genes that regulate them will provide clues on the biochemical pathways that help the seals tolerate these extreme conditions. The project allows for documentation of individual seal dives and provisioning of such information to the broader science community that seeks to study these seals, educating graduate and undergraduate students and a post-doctoral researcher and producing a science-outreach comic book for middle-school students to illustrate the project\u0027s science activities, goals and outcomes. Part II: Technical description: The Weddell seal is a champion diver with high natural tolerance for low blood oxygen concentration (hypoxemia) and inadequate blood supply (ischemia). The processes unique to this species protects their tissues from inflammation and oxidative stress observed in other mammalian tissues exposed to such physiological conditions. This project aims to understand the signatures of the processes that protect seals from inflammation and oxidant stress, using molecular, cellular and metabolic tools. Repetitive short dives before long ones are hypothesized to precondition seal tissues and activate the protective processes. The new aspect of this work is the study of endothelial cells, which sense changes in oxygen and blood flow, providing a link between breath-holding and cellular function. The approach is one of laboratory experiments combined with 2-years of field work in an ice camp off McMurdo Station in Antarctica. The study is structured by three main objectives: 1) laboratory experiments with arterial endothelial cells exposed to changes in oxygen and flow to identify molecular pathways responsible for tolerance of hypoxia and ischemia using several physiological, biochemical and genomic tools including CRSPR/Cas9 knochout and knockdown approaches. 2) Metabolomic analyses of blood metabolites produced by seals during long dives. And 3) Metabolomic and genomic determinations of seal physiology during short dives hypothesized to pre-condition tolerance responses. In the field, blood samples will be taken after seals dive in an isolated ice hole and its diving performance recorded. It is expected that the blood will contain metabolites that can be related to molecular pathways identified in lab experiments. Expert collaborators will provide field support, with the ice camp, dive hole for the seals, and telemetry associated with the seals\u2019 dives. The project builds upon previous NSF-funded projects where the seal genome and cellular resources were produced. Undergraduate researchers will be recruited from institutional programs with a track record of attracting underrepresented minorities and a minority-serving institution. To further increase polar literacy training and educational impacts, the field team will include a blog where field experiences are shared and comic book preparation with an artist designed for K-12 students and public outreach. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 167.0, "geometry": "POINT(165.5 -77.525)", "instruments": null, "is_usap_dc": true, "keywords": "FIELD SURVEYS; USA/NSF; AMD; MAMMALS; McMurdo Sound; Amd/Us", "locations": "McMurdo Sound", "north": -77.2, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Hindle, Allyson", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS", "repositories": null, "science_programs": null, "south": -77.85, "title": "Collaborative Research: Role of Endothelial Cell Activation in Hypoxia Tolerance of an Elite Diver, the Weddell Seal", "uid": "p0010257", "west": 164.0}, {"awards": "1954241 O\u0027\u0027Brien, Kristin", "bounds_geometry": null, "dataset_titles": null, "datasets": null, "date_created": "Tue, 17 Aug 2021 00:00:00 GMT", "description": "Part 1: Non-technical description: Global climate warming is increasing the frequency and severity of low oxygen events in marine and freshwater environments worldwide, and these events threaten the health of aquatic ecosystems and the viability of fish populations. The Southern Ocean surrounding Antarctica has historically been a stable, icy-cold, and oxygen-rich environment, but is now warming at an unprecedented rate and faster than all other regions in the Southern hemisphere. Antarctic fishes have evolved in sub-zero temperatures that have been stable over long periods of time with traits allowing them to thrive in frigid waters, but with diminished resilience to warming temperatures. Presently little is known about the ability of Antarctic fishes to withstand hypoxic, or low-oxygen, conditions that often accompany warming. This research will investigate the hypoxia tolerance of four species of Antarctic fishes, including two species of icefishes that lack the oxygen-carrying protein, hemoglobin, which may compromise their ability to oxygenate tissues under hypoxic conditions. The hypoxia tolerance of four Antarctic fish species will be compared to that of a related fish species inhabiting warmer coastal regions of South America. Physiological and biochemical responses to hypoxia will be evaluated and compared amongst the five species to bolster our predictions of the capacity of Antarctic fishes to cope with a changing environment. This research will provide training opportunities for undergraduate and graduate students, and a postdoctoral research fellow. A year-long seminar series hosted by the Aquarium of the Pacific will feature female scientists who work in Antarctica to inspire youth in the greater Los Angeles area to pursue careers in science. Part 2: Technical description: The overarching hypothesis to be tested in this project is that the long evolution of Antarctic notothenioid fishes in a cold, oxygen-rich environment has reduced their capacity to mount a robust physiological, biochemical, and molecular response to hypoxia compared to related, cold-temperate fish species. Hypoxia tolerance will be compared among the red-blooded Antarctic notothenioids, Notothenia coriiceps and Notothenia rossii; the hemoglobinless Antarctic icefishes, Chaenocephalus aceratus and Chionodraco rastrospinosus; and the basal, cold-temperate notothenioid, Eleginops maclovinus, a species that has never inhabited waters south of the Polar Front. The minimum level of oxygen required to sustain maintenance metabolic requirements (O2crit) will be quantified. Animals will then be exposed to 65% of O2crit for 48 hours, and responses to hypoxia will be evaluated by measuring hematocrit and hemoglobin levels, as well as metabolites in brain, liver, glycolytic and cardiac muscles. Maximal activities of key enzymes of aerobic and anaerobic metabolism will be quantified to assess capacities for synthesizing ATP in hypoxic conditions. Gill remodeling will be analyzed using light and scanning electron microscopy. The molecular response to hypoxia will be characterized in liver and brains by quantifying levels of the master transcriptional regulator of oxygen homeostasis, hypoxia-inducible factor-1 (HIF-1), and hypoxic gene expression will be quantified using RNA-Seq. Cell cultures will be used to determine if a previously identified insertion mutation in notothenioid HIF-1 affects the ability of HIF-1 to drive gene expression and thus, hypoxia tolerance. The results of this project will provide the most comprehensive assessment of the hypoxia tolerance of Antarctic fishes to date. Broader impacts include research training opportunities for undergraduate and graduate students and a postdoctoral research associate, with a focus on involving Native Alaskan students in research. In partnership with the Aquarium of the Pacific, a year-long public seminar series will be held, showcasing the research and careers of 9 women who conduct research in Antarctica. The goal of the series is to cultivate and empower a community of middle and high school students in the greater Los Angeles area to pursue their interests in science and related fields, and to enhance the public engagement capacities of research scientists so that they may better inspire youth and early career scientists in STEM fields. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "Palmer Station; FIELD SURVEYS; USAP-DC; AMD; USA/NSF; Amd/Us; FISH", "locations": "Palmer Station", "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "O\u0027Brien, Kristin", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS", "repositories": null, "science_programs": null, "south": null, "title": "ANT LIA: Hypoxia Tolerance in Notothenioid Fishes", "uid": "p0010246", "west": null}, {"awards": "1643532 Ponganis, Paul", "bounds_geometry": "POLYGON((163 -77,163.4 -77,163.8 -77,164.2 -77,164.6 -77,165 -77,165.4 -77,165.8 -77,166.2 -77,166.6 -77,167 -77,167 -77.1,167 -77.2,167 -77.3,167 -77.4,167 -77.5,167 -77.6,167 -77.7,167 -77.8,167 -77.9,167 -78,166.6 -78,166.2 -78,165.8 -78,165.4 -78,165 -78,164.6 -78,164.2 -78,163.8 -78,163.4 -78,163 -78,163 -77.9,163 -77.8,163 -77.7,163 -77.6,163 -77.5,163 -77.4,163 -77.3,163 -77.2,163 -77.1,163 -77))", "dataset_titles": "Blood oxygen transport and depletion in diving emperor penguins; Emperor penguin air sac oxygen", "datasets": [{"dataset_uid": "200236", "doi": "10.5061/dryad.3tx95x6f5", "keywords": null, "people": null, "repository": "Dryad", "science_program": null, "title": "Emperor penguin air sac oxygen", "url": "https://doi.org/10.6076/D1H01Z"}, {"dataset_uid": "200409", "doi": "10.5061/dryad.qv9s4mwnp", "keywords": null, "people": null, "repository": "Dryad", "science_program": null, "title": "Blood oxygen transport and depletion in diving emperor penguins", "url": "https://doi.org/10.5061/dryad.qv9s4mwnp"}], "date_created": "Fri, 30 Jul 2021 00:00:00 GMT", "description": "During exercise, oxygen must be efficiently delivered from the lungs to the working tissues. Birds have a unique respiratory system that includes both air sacs and lungs (called parabronchi) and has a one-way, rather than bidirectional, air flow pattern. This allows a high proportion of the oxygen in inhaled air to be transferred into the blood so that it can be circulated by the cardiovascular system to the tissues. In diving birds such as the emperor penguin, the air sac-to-tissue oxygen delivery is essential to the dive capacity, and is one of the adaptations that allows this species to dive deeper than 500 meters. However, the physiological mechanisms underlying the transfer of oxygen from air sacs to blood and the subsequent distribution of oxygen to tissues are poorly understood. The emperor penguin is ideal for investigation of this oxygen cascade because of its large body size, dive capacity, physiological data base, and the prior development of research techniques and protocols for this species. This study should provide insight into a) the mechanisms underlying the efficiency of the bird oxygen transport system, b) the physiological basis of penguin dive behavior, and the ability of penguins to adapt to environmental change, and c) perhaps, even the design of better therapeutic strategies and tools for treatment of respiratory disease. The project also includes educational exhibits and lecture programs on penguin biology at SeaWorld of San Diego. These educational programs at SeaWorld have outreach to diverse groups of grade school and high school students. One graduate student will also be trained, and participate in Antarctic physiological research. This project will examine the transport of oxygen from air sacs to tissues in a series of studies with temporarily captive emperor penguins that are free-diving at an isolated dive hole research camp in McMurdo Sound. Physiological data will be obtained with application of backpack recorders for the partial pressure of oxygen (PO2) in air sacs and/or blood, and backpack heart rate/stroke rate recorders. This experimental approach will lay the groundwork for future investigations of air sac to lung to blood oxygen transfer during exercise of flying and running birds. Four major topics are examined in this project: a) air sac oxygen distribution/depletion and the movement of air between anterior and posterior air sacs, b) anterior air sac to arterial PO2 differences and parabronchial gas exchange, c) blood oxygen transport and depletion throughout dives, and the nature of the aerobic dive limit, and d) the relationship of venous oxygen depletion patterns to both heart rate and stroke effort during dives. Specific educational outreach goals include a) short video features to be displayed in the Penguin Encounter exhibit at SeaWorld of San Diego, and b) lectures, video presentations, and pre- and post-course evaluations for student campers and participants in SeaWorld\u0027s education programs. Underwater video for exhibits/presentations with be obtained with use of a penguin backpack camera in the Antarctic. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 167.0, "geometry": "POINT(165 -77.5)", "instruments": null, "is_usap_dc": true, "keywords": "McMurdo Sound; USAP-DC; FIELD SURVEYS; USA/NSF; Amd/Us; AMD; PENGUINS", "locations": "McMurdo Sound", "north": -77.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Ponganis, Paul", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS", "repo": "Dryad", "repositories": "Dryad", "science_programs": null, "south": -78.0, "title": "From Air Sacs to Tissues: Oxygen Transfer and Utilization in Diving Emperor Penguins", "uid": "p0010236", "west": 163.0}, {"awards": "1745130 Moran, Amy", "bounds_geometry": "POLYGON((163 -76,163.3 -76,163.6 -76,163.9 -76,164.2 -76,164.5 -76,164.8 -76,165.1 -76,165.4 -76,165.7 -76,166 -76,166 -76.2,166 -76.4,166 -76.6,166 -76.8,166 -77,166 -77.2,166 -77.4,166 -77.6,166 -77.8,166 -78,165.7 -78,165.4 -78,165.1 -78,164.8 -78,164.5 -78,164.2 -78,163.9 -78,163.6 -78,163.3 -78,163 -78,163 -77.8,163 -77.6,163 -77.4,163 -77.2,163 -77,163 -76.8,163 -76.6,163 -76.4,163 -76.2,163 -76))", "dataset_titles": "Benthic seawater temperature and conductivity measurements at six sites in McMurdo Sound; Effect of temperature on cleavage rate of Antarctic invertebrates; Effect of temperature on oxygen consumption rates of larvae of four Antarctic marine invertebrates; Egg diameters of Colossendeis megalonyx; Survey Metadata. All counts of Odontaster validus from SSWS surveys at the McMurdo Intake Jetty and Cinder Cones.; Temperature acclimation and acclimatization of sea spider larvae; Temperature effects on proximal composition and development rate of embryos and larvae of four Antarctic invertebrates; Video of Colossendeis megalonyx behavior around egg mass", "datasets": [{"dataset_uid": "601888", "doi": "10.15784/601888", "keywords": "Antarctica; Cryosphere; McMurdo; Temperature", "people": "Lobert, Graham; Toh, MIng Wei Aaron; Moran, Amy", "repository": "USAP-DC", "science_program": null, "title": "Effect of temperature on oxygen consumption rates of larvae of four Antarctic marine invertebrates", "url": "https://www.usap-dc.org/view/dataset/601888"}, {"dataset_uid": "601889", "doi": "10.15784/601889", "keywords": "Antarctica; Cryosphere; McMurdo; Temperature", "people": "Moran, Amy; Lobert, Graham; Toh, MIng Wei Aaron", "repository": "USAP-DC", "science_program": null, "title": "Temperature acclimation and acclimatization of sea spider larvae", "url": "https://www.usap-dc.org/view/dataset/601889"}, {"dataset_uid": "601869", "doi": null, "keywords": "Antarctica; Cryosphere; McMurdo; McMurdo Sound", "people": "Thurber, Andrew; Moran, Amy", "repository": "USAP-DC", "science_program": null, "title": "Survey Metadata. All counts of Odontaster validus from SSWS surveys at the McMurdo Intake Jetty and Cinder Cones.", "url": "https://www.usap-dc.org/view/dataset/601869"}, {"dataset_uid": "601886", "doi": "10.15784/601886", "keywords": "Antarctica; Cryosphere; McMurdo; Temperature", "people": "Toh, Ming Wei Aaron; Lobert, Graham; Moran, Amy", "repository": "USAP-DC", "science_program": null, "title": "Temperature effects on proximal composition and development rate of embryos and larvae of four Antarctic invertebrates", "url": "https://www.usap-dc.org/view/dataset/601886"}, {"dataset_uid": "601870", "doi": "10.15784/601870", "keywords": "Antarctica; Cryosphere; McMurdo Sound; Salinity; Temperature", "people": "Moran, Amy", "repository": "USAP-DC", "science_program": null, "title": "Benthic seawater temperature and conductivity measurements at six sites in McMurdo Sound", "url": "https://www.usap-dc.org/view/dataset/601870"}, {"dataset_uid": "601716", "doi": "10.15784/601716", "keywords": "Antarctica; McMurdo; Pycnogonida; Sea Spider", "people": "Moran, Amy; Lobert, Graham", "repository": "USAP-DC", "science_program": null, "title": "Video of Colossendeis megalonyx behavior around egg mass", "url": "https://www.usap-dc.org/view/dataset/601716"}, {"dataset_uid": "601717", "doi": "10.15784/601717", "keywords": "Antarctica; McMurdo", "people": "Moran, Amy", "repository": "USAP-DC", "science_program": null, "title": "Egg diameters of Colossendeis megalonyx", "url": "https://www.usap-dc.org/view/dataset/601717"}, {"dataset_uid": "601887", "doi": "10.15784/601887", "keywords": "Antarctica; Cryosphere; McMurdo; Temperature", "people": "Moran, Amy; Lobert, Graham; Toh, Ming Wei Aaron", "repository": "USAP-DC", "science_program": null, "title": "Effect of temperature on cleavage rate of Antarctic invertebrates", "url": "https://www.usap-dc.org/view/dataset/601887"}], "date_created": "Wed, 09 Jun 2021 00:00:00 GMT", "description": "Cold-blooded animals in the Antarctic ocean have survived in near-constant, extreme cold conditions for millions of years and are very sensitive to even small changes in water temperature. However, the consequences of this extreme thermal sensitivity for the energetics, development, and survival of developing embryos is not well understood. This award will investigate the effect of temperature on the metabolism, growth rate, developmental rate, and developmental energetics of embryos and larvae of Antarctic marine ectotherms. The project will also measure annual variation in temperature and oxygen at different sites in McMurdo Sound, and compare embryonic and larval metabolism in winter and summer to determine the extent to which these life stages can acclimate to seasonal shifts. This research will provide insight into the ability of polar marine animals and ecosystems to withstand warming polar ocean conditions. Antarctic marine ectotherms exhibit universally slow growth, low metabolic rates, and extended development, yet many of their rate processes related to physiology and metabolism are highly thermally sensitive. This suggests that small changes in temperature may result in dramatic changes to energy metabolism, growth, and the rate and duration of development. This project will measure the effects of temperature on metabolism, developmental rate, and the energetic cost of development of four common and ecologically important species of benthic Antarctic marine invertebrates. These effects will be measured over the functional ranges of the organisms and in the context of environmentally relevant seasonal shifts in temperature around McMurdo Sound. Recent data show that seasonal warming of ~1 deg C near McMurdo Station is accompanied by long-lasting hyperoxic events that impact the benthos in the nearshore boundary layer. This research will provide a more comprehensive understanding of both annual variation in environmental oxygen and temperature across the Sound, and whether this variation drives changes in developmental rate and energetics that are consistent with physiological acclimatization. These data will provide key information about potential impacts of warming Antarctic ectotherms. In addition, this project will support undergraduate and graduate research and partner with large-enrollment undergraduate courses and REU programs at an ANNH and AANAPISI Title III minority-serving institution. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 166.0, "geometry": "POINT(164.5 -77)", "instruments": null, "is_usap_dc": true, "keywords": "USAP-DC; Amd/Us; McMurdo Sound; AMD; BENTHIC; USA/NSF; FIELD INVESTIGATION", "locations": "McMurdo Sound", "north": -76.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Moran, Amy", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -78.0, "title": "Thermal Sensitivity of Antarctic Embryos and Larvae: Effects of Temperature on Metabolism, Developmental Rate, and the Metabolic Cost of Development ", "uid": "p0010187", "west": 163.0}, {"awards": "1142158 Cheng, Chi-Hing; 0231006 DeVries, Arthur", "bounds_geometry": "POLYGON((163 -76.5,163.5 -76.5,164 -76.5,164.5 -76.5,165 -76.5,165.5 -76.5,166 -76.5,166.5 -76.5,167 -76.5,167.5 -76.5,168 -76.5,168 -76.63,168 -76.76,168 -76.89,168 -77.02,168 -77.15,168 -77.28,168 -77.41,168 -77.54,168 -77.67,168 -77.8,167.5 -77.8,167 -77.8,166.5 -77.8,166 -77.8,165.5 -77.8,165 -77.8,164.5 -77.8,164 -77.8,163.5 -77.8,163 -77.8,163 -77.67,163 -77.54,163 -77.41,163 -77.28,163 -77.15,163 -77.02,163 -76.89,163 -76.76,163 -76.63,163 -76.5))", "dataset_titles": "High-resolution benthic seawater temperature record 1999-2012 (25-40m depth) from near intake jetty at McMurdo Station, Antarctica; Metadata associated with the description of Akarotaxis gouldae n. sp. (Bathydraconidae)", "datasets": [{"dataset_uid": "601811", "doi": null, "keywords": "Antarctica; Bellingshausen Sea; Cryosphere; Southern Ocean", "people": "Biesack, Ellen; Corso, Andrew; Desvignes, Thomas; McDowell, Jan; Cheng, Chi-Hing; Steinberg, Deborah; Hilton, Eric", "repository": "USAP-DC", "science_program": "LTER", "title": "Metadata associated with the description of Akarotaxis gouldae n. sp. (Bathydraconidae)", "url": "https://www.usap-dc.org/view/dataset/601811"}, {"dataset_uid": "601275", "doi": null, "keywords": "Antarctica; Benthic; McMurdo Sound; Mcmurdo Station; Oceans; Physical Oceanography; Temperature Probe; Water Temperature", "people": "Cziko, Paul; Devries, Arthur; Cheng, Chi-Hing", "repository": "USAP-DC", "science_program": null, "title": "High-resolution benthic seawater temperature record 1999-2012 (25-40m depth) from near intake jetty at McMurdo Station, Antarctica", "url": "https://www.usap-dc.org/view/dataset/601275"}], "date_created": "Wed, 08 Apr 2020 00:00:00 GMT", "description": "Antarctic notothenioid fishes exhibit two adaptive traits to survive in frigid temperatures. The first of these is the production of anti-freeze proteins in their blood and tissues. The second is a system-wide ability to perform cellular and physiological functions at extremely cold temperatures.The proposal goals are to show how Antarctic fishes use these characteristics to avoid freezing, and which additional genes are turned on, or suppressed in order for these fishes to maintain normal physiological function in extreme cold temperatures. Progressively colder habitats are encountered in the high latitude McMurdo Sound and Ross Shelf region, along with somewhat milder near?shore water environments in the Western Antarctic Peninsula (WAP). By quantifying the extent of ice crystals invading and lodging in the spleen, the percentage of McMurdo Sound fish during austral summer (Oct-Feb) will be compared to the WAP intertidal fish during austral winter (Jul-Sep) to demonstrate their capability and extent of freeze avoidance. Resistance to ice entry in surface epithelia (e.g. skin, gill and intestinal lining) is another expression of the adaptation of these fish to otherwise lethally freezing conditions. The adaptive nature of a uniquely characteristic polar genome will be explored by the study of the transcriptome (the set of expressed RNA transcripts that constitutes the precursor to set of proteins expressed by an entire genome). Three notothenioid species (E.maclovinus, D. Mawsoni and C. aceratus) will be analysed to document evolutionary genetic changes (both gain and loss) shaped by life under extreme chronic cold. A differential gene expression (DGE) study will be carried out on these different species to evaluate evolutionary modification of tissue-wide response to heat challenges. The transcriptomes and other sequencing libraries will contribute to de novo ice-fish genome sequencing efforts.", "east": 168.0, "geometry": "POINT(165.5 -77.15)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD", "is_usap_dc": true, "keywords": "McMurdo Sound; MARINE ECOSYSTEMS; Water Temperature; AQUATIC SCIENCES; OCEAN TEMPERATURE; FIELD INVESTIGATION; USAP-DC", "locations": "McMurdo Sound", "north": -76.5, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Cheng, Chi-Hing; Devries, Arthur", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -77.8, "title": "Antarctic Notothenioid Fish Freeze Avoidance and Genome-wide Evolution for Life in the Cold", "uid": "p0010091", "west": 163.0}, {"awards": "1341602 Crockett, Elizabeth; 1341663 O\u0027Brien, Kristin", "bounds_geometry": null, "dataset_titles": "Acclimation of cardiovascular function in Notothenia coriiceps; Adrenergic and adenosinergic regulation of the cardiovascular system in the Antarctic icefish Chaenocephalus aceratus; Cardiac metabolism in Antarctic fishes in response to an acute increase in temperature; Chaenocephalus aceratus HIF-1A mRNA, complete cds; Chionodraco rastrospinosus HIF-1A mRNA, partial cds; Effects of acute warming on cardiovascular performance of Antarctic fishes; Eleginops maclovinus HIF-1A mRNA, partial cds; Gymnodraco acuticeps HIF-1A mRNA, partial cds; Hypoxia response of hearts of Antarctic fishes; Maximum cardiac performance of Antarctic fishes that lack haemoglobin and myoglobin: exploring the effect of warming on nature\u2019s natural knockouts; Measurements of splenic contraction in Antarctic fishes; Mitochondrial membranes in cardiac muscle from Antarctic notothenioid fishes vary in phospholipid composition and membrane fluidity; Notothenia coriiceps HIF-1A mRNA, complete cds; Parachaenichthys charcoti HIF-1A mRNA, partial cds; Physical, chemical, and functional properties of neuronal membranes vary between species of Antarctic notothenioids differing in thermal tolerance; Thermal sensitivity of membrane fluidity and integrity in hearts of Antarctic fishes that vary in expression of hemoglobin and myoglobin", "datasets": [{"dataset_uid": "601406", "doi": "10.15784/601406", "keywords": "Antarctica; Antarctic Peninsula", "people": "O\u0027Brien, Kristin", "repository": "USAP-DC", "science_program": null, "title": "Hypoxia response of hearts of Antarctic fishes", "url": "https://www.usap-dc.org/view/dataset/601406"}, {"dataset_uid": "601405", "doi": "10.15784/601405", "keywords": "Antarctica; Antarctic Peninsula", "people": "O\u0027Brien, Kristin", "repository": "USAP-DC", "science_program": null, "title": "Cardiac metabolism in Antarctic fishes in response to an acute increase in temperature", "url": "https://www.usap-dc.org/view/dataset/601405"}, {"dataset_uid": "200192", "doi": null, "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "Chionodraco rastrospinosus HIF-1A mRNA, partial cds", "url": "https://www.ncbi.nlm.nih.gov/nuccore/kx950831"}, {"dataset_uid": "200185", "doi": "10.5061/dryad.k90h35k", "keywords": null, "people": null, "repository": "Dryad", "science_program": null, "title": "Mitochondrial membranes in cardiac muscle from Antarctic notothenioid fishes vary in phospholipid composition and membrane fluidity", "url": "https://doi.org/10.5061/dryad.k90h35k"}, {"dataset_uid": "200186", "doi": "10.5061/dryad.qm0b25h", "keywords": null, "people": null, "repository": "Dryad", "science_program": null, "title": "Physical, chemical, and functional properties of neuronal membranes vary between species of Antarctic notothenioids differing in thermal tolerance", "url": "https://doi.org/10.5061/dryad.qm0b25h"}, {"dataset_uid": "200187", "doi": null, "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "Chaenocephalus aceratus HIF-1A mRNA, complete cds", "url": "https://www.ncbi.nlm.nih.gov/nuccore/KX950828"}, {"dataset_uid": "200188", "doi": null, "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "Notothenia coriiceps HIF-1A mRNA, complete cds", "url": "https://www.ncbi.nlm.nih.gov/nuccore/KX950829"}, {"dataset_uid": "200189", "doi": null, "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "Eleginops maclovinus HIF-1A mRNA, partial cds", "url": "https://www.ncbi.nlm.nih.gov/nuccore/KX950830"}, {"dataset_uid": "601410", "doi": "10.15784/601410", "keywords": "Antarctica; Antarctic Peninsula; Biota; Fish", "people": "O\u0027Brien, Kristin; Crockett, Elizabeth; Egginton, Stuart; Axelsson, Michael; Farrell, Anthony; Joyce, William", "repository": "USAP-DC", "science_program": null, "title": "Effects of acute warming on cardiovascular performance of Antarctic fishes", "url": "https://www.usap-dc.org/view/dataset/601410"}, {"dataset_uid": "601409", "doi": "10.15784/601409", "keywords": "Antarctica; Antarctic Peninsula", "people": "Joyce, Michael; Axelsson, Michael; Farrell, Anthony; Egginton, Stuart; O\u0027Brien, Kristin", "repository": "USAP-DC", "science_program": null, "title": "Adrenergic and adenosinergic regulation of the cardiovascular system in the Antarctic icefish Chaenocephalus aceratus", "url": "https://www.usap-dc.org/view/dataset/601409"}, {"dataset_uid": "601408", "doi": "10.15784/601408", "keywords": "Antarctica; Antarctic Peninsula", "people": "Crockett, Elizabeth; Joyce, William; Farrell, Anthony; Egginton, Stuart; Axelsson, Michael; O\u0027Brien, Kristin", "repository": "USAP-DC", "science_program": null, "title": "Acclimation of cardiovascular function in Notothenia coriiceps", "url": "https://www.usap-dc.org/view/dataset/601408"}, {"dataset_uid": "601407", "doi": "10.15784/601407", "keywords": "Antarctica; Antarctic Peninsula", "people": "Axelsson, Michael; O\u0027Brien, Kristin; Joyce, William", "repository": "USAP-DC", "science_program": null, "title": "Measurements of splenic contraction in Antarctic fishes", "url": "https://www.usap-dc.org/view/dataset/601407"}, {"dataset_uid": "200191", "doi": null, "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "Gymnodraco acuticeps HIF-1A mRNA, partial cds", "url": "https://www.ncbi.nlm.nih.gov/nuccore/kx950832"}, {"dataset_uid": "601414", "doi": "10.15784/601414", "keywords": "Antarctica; Antarctic Peninsula", "people": "Crockett, Elizabeth; O\u0027Brien, Kristin; Evans, Elizabeth; Farnoud, Amir", "repository": "USAP-DC", "science_program": null, "title": "Thermal sensitivity of membrane fluidity and integrity in hearts of Antarctic fishes that vary in expression of hemoglobin and myoglobin", "url": "https://www.usap-dc.org/view/dataset/601414"}, {"dataset_uid": "200190", "doi": null, "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "Parachaenichthys charcoti HIF-1A mRNA, partial cds", "url": "https://www.ncbi.nlm.nih.gov/nuccore/KX950833"}, {"dataset_uid": "200184", "doi": "10.5061/dryad.83vc5", "keywords": null, "people": null, "repository": "Dryad", "science_program": null, "title": "Maximum cardiac performance of Antarctic fishes that lack haemoglobin and myoglobin: exploring the effect of warming on nature\u2019s natural knockouts", "url": "https://doi.org/10.5061/dryad.83vc5"}], "date_created": "Wed, 26 Feb 2020 00:00:00 GMT", "description": "The ocean surrounding Antarctica is home to an extraordinary assemblage of fishes, dominated by a single group that are extremely well-suited to life in icy waters and which are of significant ecological importance there. Of great concern is the capacity of these fishes to withstand increases in temperature as the region of the Western Antarctic Peninsula warms at a rate faster than any other area in the Southern hemisphere. One particular group of Antarctic fishes, known as the icefishes, are particularly vulnerable to increases in temperature because unlike all other vertebrates on earth, icefishes are white-blooded due to their lack of the oxygen-binding protein hemoglobin. This greatly reduces their capacity to transport and deliver oxygen to tissues compared to red-blooded Antarctic fishes. Previous studies have shown that icefishes are indeed less tolerant to elevations in temperature but the underlying factors are completely unknown. Additionally, it is not understood if red- or white-blooded Antarctic fishes can adjust, or acclimate, to modest increases in temperature, similar to those changes in temperature the animals might experience as the earth warms. The investigators will determine if heart function and/or nervous system function limits thermal tolerance of Antarctic fishes, and will determine their capacity to acclimate to warmer temperatures. The project will further the NSF goal of training new generations of scientists by training graduate and undergraduate students. In addition, the project will collaborate with a high school biology teacher from a school which serves a largely minority student body. The students will learn about the marine environment, and will construct a camera to be used in the field to learn more about Antarctic fishes. Two students and the teacher will also attend a summer marine biology internship program. Antarctic fishes within the suborder Notothenioidei (called \"notothenioids\") are among the organisms on earth least able to deal with changes in temperature. The hemoglobinless icefish are even less able to withstand temperature changes than are red-blooded notothenioids. While this is well documented, the underlying physiological and biochemical mechanisms responsible are unknown. The investigators will test the hypotheses that cardiac work is significantly greater in icefishes compared to red-blooded species, and that as temperature increases, the greater cardiac work of icefishes, coupled with reduced blood oxygen-carrying capacity, results in cardiac failure at a lower temperature compared to red-blooded species. They also hypothesize that neuronal function limits thermal tolerance of red-blooded notothenioids. These hypotheses will be tested using a wide variety of experiments. For example, the investigators will measure heart rate concurrently with critical thermal maximum. They will also characterize metabolic and gene-expression responses to elevated temperature and determine if mitochondrial function contributes to thermal tolerance using a variety of techniques. To determine if neuronal function limits thermal tolerance they will quantify behavioral responses to warming of whole animals and to warming of only the brain area. They will also determine if acclimation to warmer temperatures impacts heart function and they will measure activities of a variety of enzymes from central metabolic pathways.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "Antarctica; Amd/Us; FISH; USA/NSF; FIELD INVESTIGATION; AMD; Antarctic Peninsula; LABORATORY; USAP-DC", "locations": "Antarctica; Antarctic Peninsula", "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Crockett, Elizabeth; O\u0027Brien, Kristin", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION; OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repo": "USAP-DC", "repositories": "Dryad; GenBank; USAP-DC", "science_programs": null, "south": null, "title": "Collaborative Research: The Physiological and Biochemical Underpinnings of Thermal Tolerance in Antarctic Notothenioid Fishes", "uid": "p0010084", "west": null}, {"awards": "1543383 Postlethwait, John", "bounds_geometry": "POLYGON((-66 -62,-65.2 -62,-64.4 -62,-63.6 -62,-62.8 -62,-62 -62,-61.2 -62,-60.4 -62,-59.6 -62,-58.8 -62,-58 -62,-58 -62.4,-58 -62.8,-58 -63.2,-58 -63.6,-58 -64,-58 -64.4,-58 -64.8,-58 -65.2,-58 -65.6,-58 -66,-58.8 -66,-59.6 -66,-60.4 -66,-61.2 -66,-62 -66,-62.8 -66,-63.6 -66,-64.4 -66,-65.2 -66,-66 -66,-66 -65.6,-66 -65.2,-66 -64.8,-66 -64.4,-66 -64,-66 -63.6,-66 -63.2,-66 -62.8,-66 -62.4,-66 -62))", "dataset_titles": "C. aceratus pronephric kidney (head kidney) miRNA; mirtop\r\ncommand lines tool to annotate miRNAs with a standard mirna/isomir naming; Patagonotothen cornucola isolate Pcor_18_01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial; Patagonotothen sima isolate Psim_18_11 cardiac muscle myosin heavy chain 6 (myh6) gene, partial cds; Patagonotothen sima isolate Psim_18_11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial; Patagonotothen sima isolate Psim_18_12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial; Prost!, a tool for miRNA annotation and next generation smallRNA sequencing experiment analysis; Quantifying expression levels of smallRNAs between tissues in Danio Rerio strain AB.; Quantifying expression levels of smallRNAs between tissues in three-spined stickleback", "datasets": [{"dataset_uid": "200131", "doi": "", "keywords": null, "people": null, "repository": "GitHub", "science_program": null, "title": "Prost!, a tool for miRNA annotation and next generation smallRNA sequencing experiment analysis", "url": "https://github.com/uoregon-postlethwait/prost"}, {"dataset_uid": "200130", "doi": "", "keywords": null, "people": null, "repository": "NCBI SRA", "science_program": null, "title": "C. aceratus pronephric kidney (head kidney) miRNA", "url": "https://www.ncbi.nlm.nih.gov/search/all/?term=SRP069031"}, {"dataset_uid": "200129", "doi": "", "keywords": null, "people": null, "repository": "NCBI SRA", "science_program": null, "title": "Quantifying expression levels of smallRNAs between tissues in Danio Rerio strain AB.", "url": "https://www.ncbi.nlm.nih.gov/search/all/?term=SRP039502"}, {"dataset_uid": "200136", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Patagonotothen sima isolate Psim_18_11 cardiac muscle myosin heavy chain 6 (myh6) gene, partial cds", "url": "https://www.ncbi.nlm.nih.gov/search/all/?term=MN136234+"}, {"dataset_uid": "200135", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Patagonotothen sima isolate Psim_18_12 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial", "url": "https://www.ncbi.nlm.nih.gov/search/all/?term=MN136233+"}, {"dataset_uid": "200128", "doi": "", "keywords": null, "people": null, "repository": "NCBI SRA", "science_program": null, "title": "Quantifying expression levels of smallRNAs between tissues in three-spined stickleback", "url": "https://www.ncbi.nlm.nih.gov/search/all/?term=SRP157992"}, {"dataset_uid": "200132", "doi": "", "keywords": null, "people": null, "repository": "GitHub", "science_program": null, "title": "mirtop\r\ncommand lines tool to annotate miRNAs with a standard mirna/isomir naming", "url": "https://github.com/miRTop"}, {"dataset_uid": "200134", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Patagonotothen sima isolate Psim_18_11 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial", "url": "https://www.ncbi.nlm.nih.gov/search/all/?term=MN136232"}, {"dataset_uid": "200133", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Patagonotothen cornucola isolate Pcor_18_01 cytochrome oxidase subunit 1 (COI) gene, partial cds; mitochondrial", "url": "https://www.ncbi.nlm.nih.gov/search/all/?term=MN136231"}], "date_created": "Wed, 26 Feb 2020 00:00:00 GMT", "description": "Icefish live in frigid Antarctic seas, and have unique traits such as the absence of red blood cells, enlarged hearts, large diameter blood vessels, low bone mineral densities, and fat droplets that disrupt their muscles. These features would be harmful in other animals. In mammals and fish inhabiting warm waters, development of organs involved in these traits is modulated by genes that encode specific proteins, but the rate of protein production is often regulated by short RNA molecules called microRNAs (miRNAs). Genes that code for proteins must first make an RNA copy, and the actual protein is made from this RNA copy intermediate. MiRNAs regulate the amount of protein that is made by binding to the RNA intermediate and interrupting its production of protein. Binding of miRNAs to RNA depends strongly on temperature. Regulation of genes by miRNAs has not been studied in Antarctic fish, which live in seas with temperatures below the freezing point of fresh water. This project will compare miRNA regulation 1) in Antarctic fish vs. warm-water fish to learn how miRNAs regulate gene expression in constant cold; and 2) in Antarctic icefish with no red blood cells, enlarged hearts, and reduced bone density vs. closely related Antarctic fish containing red blood cells, normal hearts, and dense bones. The project will have broad impacts to science and society nationally and globally. First, this will be the first study of important factors in gene regulation (miRNAs) in Antarctic fish, which are an essential component of the entire ecology of the Southern Ocean, and will shed light on how these fish might respond to the warming of Antarctic waters. Second, it will bring Antarctic science to under-represented high school students at a local alternative downtown high school by conducting video conferences during the Antarctic field seasons and hosting student investigations of Antarctic fish in the research laboratory. microRNAs (miRNAs) are key post-transcriptional regulators of gene expression that modulate development and physiology in temperate animals. Although miRNAs act by binding to messenger RNAs (mRNAs), a process that is strongly sensitive to temperature, miRNAs have yet not been studied in Antarctic animals, including Notothenioid fish, which dominate the Southern Ocean. This project will compare miRNA regulation in 1) Antarctic vs. temperate fish to learn the roles of miRNA regulation in adaptation to constant cold; and in 2) bottom-dwelling, dense-boned, red-blooded Nototheniods vs. high buoyancy, osteopenic, white-blooded icefish to understand miRNA regulation in specialized organs after the evolution of the loss of hemoglobin genes and red blood cells, the origin of enlarged heart and vasculature, and the evolution of increased buoyancy, which arose by decreased bone mineralization and increased lipid deposition. Aim 1 is to test the hypothesis that Antarctic fish evolved miRNA-related genome specializations in response to constant cold. The project will compare four Antarctic Notothenioid species to two temperate Notothenioids and two temperate laboratory species to test the hypotheses that (a) Antarctic fish evolved miRNA genome repertoires by loss of ancestral genes and/or gain of new genes, (b) express miRNAs that are involved in cold tolerance, and (c) respond to temperature change by changing miRNA gene expression. Aim 2 is to test the hypothesis that the evolution of icefish from red-blooded bottom-dwelling ancestors was accompanied by an altered miRNA genomic repertoire, sequence, and/or expression. The project will test the hypotheses that (a) miRNAs in icefish evolved in sequence and/or in expression in icefish specializations, including head kidney (origin of red blood cells); heart (changes in vascular system), cranium and pectoral girdle (reduced bone mineral density); and skeletal muscle (lipid deposition), and (b) miRNAs that evolved in icefish specializations had ancestral functions related to their derived roles in icefish, as determined by functional tests of zebrafish orthologs of icefish miRNAs in developing zebrafish. The program will isolate, sequence, and determine the expression of miRNAs and mRNAs using high-throughput transcriptomics and novel software. Results will show how the microRNA system evolves in vertebrate animals pushed to physiological extremes and provide insights into the prospects of key species in the most rapidly warming part of the globe.", "east": -58.0, "geometry": "POINT(-62 -64)", "instruments": null, "is_usap_dc": true, "keywords": "USAP-DC; Palmer Station; NOT APPLICABLE; FISH", "locations": "Palmer Station", "north": -62.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Postlethwait, John; Desvignes, Thomas", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "GitHub", "repositories": "GitHub; NCBI GenBank; NCBI SRA", "science_programs": null, "south": -66.0, "title": "Antarctic Fish and MicroRNA Control of Development and Physiology", "uid": "p0010085", "west": -66.0}, {"awards": "1444167 Detrich, H. William", "bounds_geometry": "POLYGON((-70 -58,-68.5 -58,-67 -58,-65.5 -58,-64 -58,-62.5 -58,-61 -58,-59.5 -58,-58 -58,-56.5 -58,-55 -58,-55 -59.8,-55 -61.6,-55 -63.4,-55 -65.2,-55 -67,-55 -68.8,-55 -70.6,-55 -72.4,-55 -74.2,-55 -76,-56.5 -76,-58 -76,-59.5 -76,-61 -76,-62.5 -76,-64 -76,-65.5 -76,-67 -76,-68.5 -76,-70 -76,-70 -74.2,-70 -72.4,-70 -70.6,-70 -68.8,-70 -67,-70 -65.2,-70 -63.4,-70 -61.6,-70 -59.8,-70 -58))", "dataset_titles": "Assembled Contig Dat for Daane et al. (2019); E-MTAB-6759: RNA-seq across tissues in four Notothenioid species (Antarctic icefish); Expedition Data of LMG1603; Expedition Data of LMG1604; Expedition Data of LMG1605; Expedition Data of LMG1803; Expedition Data of LMG1804; Expedition Data of LMG1805; Full raw data set, computer code, and evolutionary trajectories for all species in Damsgaard et al. (2019); Histology-, CT-, ultrasound-, and MRI-scans (~2 TB) for Damsgaard et al. (2019); PRJNA420419: Genome and Transcriptome Data for Kim et al. (2019) Blackfin Icefish Genome; PRJNA531677: Sequencing Data for Daane et al. (2019); S-BSST132: Assembled Transcriptomes for Berthelot et al. (2018); SRP047484 RAD-tag Sequences of Genetically Mapped Notothenia coriiceps embryos; SRP118539: RAD-tag Sequences of Genetically Mapped Chaenocephalus aceratus Embryos; Transposable element sequences and genome sizes, refs 142597 to MF142757", "datasets": [{"dataset_uid": "200099", "doi": "10.5281/zenodo.2628936", "keywords": null, "people": null, "repository": "Zenodo", "science_program": null, "title": "Assembled Contig Dat for Daane et al. (2019)", "url": "https://zenodo.org/record/2628936#.Xegqj3dFw2w"}, {"dataset_uid": "200250", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data of LMG1603", "url": "https://www.rvdata.us/search/cruise/LMG1603"}, {"dataset_uid": "200092", "doi": "", "keywords": null, "people": null, "repository": "NCBI BioProject", "science_program": null, "title": "PRJNA420419: Genome and Transcriptome Data for Kim et al. (2019) Blackfin Icefish Genome", "url": "https://www.ncbi.nlm.nih.gov/bioproject/?term=prjna420419"}, {"dataset_uid": "200094", "doi": "", "keywords": null, "people": null, "repository": "Array Express", "science_program": null, "title": "E-MTAB-6759: RNA-seq across tissues in four Notothenioid species (Antarctic icefish)", "url": "https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-6759/"}, {"dataset_uid": "200095", "doi": "", "keywords": null, "people": null, "repository": "BioStudies", "science_program": null, "title": "S-BSST132: Assembled Transcriptomes for Berthelot et al. (2018)", "url": "https://www.ebi.ac.uk/biostudies/studies/S-BSST132"}, {"dataset_uid": "200096", "doi": "", "keywords": null, "people": null, "repository": "NCBI SRA", "science_program": null, "title": "SRP047484 RAD-tag Sequences of Genetically Mapped Notothenia coriiceps embryos", "url": "https://www.ncbi.nlm.nih.gov/sra/?term=SRP047484"}, {"dataset_uid": "200252", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data of LMG1604", "url": "https://www.rvdata.us/search/cruise/LMG1604"}, {"dataset_uid": "200102", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Transposable element sequences and genome sizes, refs 142597 to MF142757", "url": "https://www.ncbi.nlm.nih.gov/nuccore?LinkName=pubmed_nuccore\u0026from_uid=29739320"}, {"dataset_uid": "200103", "doi": "", "keywords": null, "people": null, "repository": "GitHub", "science_program": null, "title": "Full raw data set, computer code, and evolutionary trajectories for all species in Damsgaard et al. (2019)", "url": "https://github.com/elifesciences-publications/Retinaevolution"}, {"dataset_uid": "200253", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data of LMG1605", "url": "https://www.rvdata.us/search/cruise/LMG1605"}, {"dataset_uid": "200254", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data of LMG1805", "url": "https://www.rvdata.us/search/cruise/LMG1805"}, {"dataset_uid": "200098", "doi": "", "keywords": null, "people": null, "repository": "NCBI BioProject", "science_program": null, "title": "PRJNA531677: Sequencing Data for Daane et al. (2019)", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA531677"}, {"dataset_uid": "200104", "doi": "", "keywords": null, "people": null, "repository": "eLife", "science_program": null, "title": "Histology-, CT-, ultrasound-, and MRI-scans (~2 TB) for Damsgaard et al. (2019)", "url": "https://retinaevolution.bios.au.dk/eLife%20documentation/README.txt"}, {"dataset_uid": "200249", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data of LMG1803", "url": "https://www.rvdata.us/search/cruise/LMG1803"}, {"dataset_uid": "200251", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data of LMG1804", "url": "https://www.rvdata.us/search/cruise/LMG1804"}, {"dataset_uid": "200093", "doi": "", "keywords": null, "people": null, "repository": "NCBI SRA", "science_program": null, "title": "SRP118539: RAD-tag Sequences of Genetically Mapped Chaenocephalus aceratus Embryos", "url": "https://www.ncbi.nlm.nih.gov/sra/SRP118539 "}], "date_created": "Wed, 04 Dec 2019 00:00:00 GMT", "description": "Antarctic fish and their early developmental stages are an important component of the food web that sustains life in the cold Southern Ocean (SO) that surrounds Antarctica. They feed on smaller organisms and in turn are eaten by larger animals, including seals and killer whales. Little is known about how rising ocean temperatures will impact the development of Antarctic fish embryos and their growth after hatching. This project will address this gap by assessing the effects of elevated temperatures on embryo viability, on the rate of embryo development, and on the gene \"toolkits\" that respond to temperature stress. One of the two species to be studied does not produce red blood cells, a defect that may make its embryos particularly vulnerable to heat. The outcomes of this research will provide the public and policymakers with \"real world\" data that are necessary to inform decisions and design strategies to cope with changes in the Earth\u0027s climate, particularly with respect to protecting life in the SO. The project will also further the NSF goals of training new generations of scientists, including providing scientific training for undergraduate and graduate students, and of making scientific discoveries available to the general public. This includes the unique educational opportunity for undergraduates to participate in research in Antarctica and engaging the public in several ways, including the development of professionally-produced educational videos with bi-lingual closed captioning. Since the onset of cooling of the SO about 40 million years ago, evolution of Antarctic marine organisms has been driven by the development of cold temperatures. Because body temperatures of Antarctic fishes fall in a narrow range determined by their habitat (-1.9 to +2.0 C) they are particularly attractive models for understanding how organismal physiology and biochemistry have been shaped to maintain life in a cooling environment. The long-term objective of this project is to understand the capacities of Antarctic fishes to acclimatize and/or adapt to rapid oceanic warming through analysis of their underlying genetic \"toolkits.\" This objective will be accomplished through three Specific Aims: 1) assessing the effects of elevated temperatures on gene expression during development of embryos; 2) examining the effects of elevated temperatures on embryonic morphology and on the temporal and spatial patterns of gene expression; and 3) evaluating the evolutionary mechanisms that have led to the loss of the red blood cell genetic program by the white-blooded fishes. Aims 1 and 2 will be investigated by acclimating experimental embryos of both red-blooded and white-blooded fish to elevated temperatures. Differential gene expression will be examined through the use of high throughput RNA sequencing. The temporal and spatial patterns of gene expression in the context of embryonic morphology (Aim 2) will be determined by microscopic analysis of embryos \"stained\" with (hybridized to) differentially expressed gene probes revealed by Aim 1; other developmental marker genes will also be used. The genetic lesions resulting from loss of red blood cells by the white-blooded fishes (Aim 3) will be examined by comparing genes and genomes in the two fish groups.", "east": -55.0, "geometry": "POINT(-62.5 -67)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "NOT APPLICABLE; Polar; South Shetland Islands; USAP-DC; COASTAL", "locations": "Polar; South Shetland Islands", "north": -58.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Detrich, H. William", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "Zenodo", "repositories": "Array Express; BioStudies; eLife; GitHub; NCBI BioProject; NCBI GenBank; NCBI SRA; R2R; Zenodo", "science_programs": null, "south": -76.0, "title": "Antarctic Notothenioid Fishes: Sentinel Taxa for Southern Ocean Warming", "uid": "p0010073", "west": -70.0}, {"awards": "1144176 Lyons, W. Berry; 1144192 Tulaczyk, Slawek; 1727387 Mikucki, Jill; 1144177 Pettit, Erin", "bounds_geometry": "POLYGON((161.8 -77.7,161.88 -77.7,161.96 -77.7,162.04000000000002 -77.7,162.12 -77.7,162.2 -77.7,162.28 -77.7,162.36 -77.7,162.44 -77.7,162.51999999999998 -77.7,162.6 -77.7,162.6 -77.70700000000001,162.6 -77.714,162.6 -77.721,162.6 -77.728,162.6 -77.735,162.6 -77.742,162.6 -77.749,162.6 -77.756,162.6 -77.76299999999999,162.6 -77.77,162.51999999999998 -77.77,162.44 -77.77,162.36 -77.77,162.28 -77.77,162.2 -77.77,162.12 -77.77,162.04000000000002 -77.77,161.96 -77.77,161.88 -77.77,161.8 -77.77,161.8 -77.76299999999999,161.8 -77.756,161.8 -77.749,161.8 -77.742,161.8 -77.735,161.8 -77.728,161.8 -77.721,161.8 -77.714,161.8 -77.70700000000001,161.8 -77.7))", "dataset_titles": "Ablation Stake Data from of Taylor Glacier near Blood Falls; Antarctica Support 2014/2015 - C-528 Blood Falls GPS/GNSS Observations Dataset; Blood Falls, McMurdo Dry Va. International Federation of Digital Seismograph Networks. Dataset/Seismic Network; FLIR thermal imaging data near Blood Falls, Taylor Glacier; Ground Penetrating Radar Data near Blood Falls, Taylor Glacier; Ice Temperature in Shallow Boreholes Near Blood Falls at the Terminus of Taylor Glacier, McMurdo Dry Valleys, Antarctica; NCBI short read archive -Metagenomic survey of Antarctic Groundwater; Terrestrial Radar Interferometry near Blood Falls, Taylor Glacier; The Geochemistry of englacial brine from Taylor Glacier, Antarctica; Time Lapse imagery of the Blood Falls feature, Antarctica ; Vaisala Integrated Met Station near Blood Falls, Taylor Glacier", "datasets": [{"dataset_uid": "601139", "doi": "10.15784/601139", "keywords": "Antarctica; Borehole; Borehole Logging; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Ice Temperature; Snow/ice; Snow/Ice; Temperature; Temperature Profiles", "people": "Tulaczyk, Slawek", "repository": "USAP-DC", "science_program": null, "title": "Ice Temperature in Shallow Boreholes Near Blood Falls at the Terminus of Taylor Glacier, McMurdo Dry Valleys, Antarctica", "url": "https://www.usap-dc.org/view/dataset/601139"}, {"dataset_uid": "200074", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "NCBI short read archive -Metagenomic survey of Antarctic Groundwater", "url": "https://www.ncbi.nlm.nih.gov/sra/?term=SRR6667787"}, {"dataset_uid": "601179", "doi": "10.15784/601179", "keywords": "Antarctica; Chemistry:ice; Chemistry:Ice; Geochemistry; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Subglacial Brine", "people": "Lyons, W. Berry; Gardner, Christopher B.", "repository": "USAP-DC", "science_program": null, "title": "The Geochemistry of englacial brine from Taylor Glacier, Antarctica", "url": "https://www.usap-dc.org/view/dataset/601179"}, {"dataset_uid": "601169", "doi": "10.15784/601169", "keywords": "Antarctica; Basal Crevassing; Glacier Hydrology; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Infrared Imagery; Photo/video; Photo/Video; Taylor Glacier; Thermal Camera; Timelaps Images", "people": "Pettit, Erin", "repository": "USAP-DC", "science_program": null, "title": "FLIR thermal imaging data near Blood Falls, Taylor Glacier", "url": "https://www.usap-dc.org/view/dataset/601169"}, {"dataset_uid": "601168", "doi": "10.15784/601168", "keywords": "Antarctica; Atmosphere; Basal Crevassing; Glacier Hydrology; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Meteorology; Taylor Glacier; Temperature; Weather Station Data; Wind Speed", "people": "Pettit, Erin", "repository": "USAP-DC", "science_program": null, "title": "Vaisala Integrated Met Station near Blood Falls, Taylor Glacier", "url": "https://www.usap-dc.org/view/dataset/601168"}, {"dataset_uid": "601167", "doi": "10.15784/601167", "keywords": "Antarctica; Basal Crevassing; Glacier Hydrology; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Photo; Photo/video; Photo/Video; Snow/ice; Snow/Ice; Taylor Glacier; Timelaps Images", "people": "Pettit, Erin", "repository": "USAP-DC", "science_program": null, "title": "Time Lapse imagery of the Blood Falls feature, Antarctica ", "url": "https://www.usap-dc.org/view/dataset/601167"}, {"dataset_uid": "601166", "doi": "10.15784/601166", "keywords": "Antarctica; Basal Crevassing; Glacier Hydrology; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; GPR; Radar; Snow/ice; Snow/Ice; Taylor Glacier", "people": "Pettit, Erin", "repository": "USAP-DC", "science_program": null, "title": "Terrestrial Radar Interferometry near Blood Falls, Taylor Glacier", "url": "https://www.usap-dc.org/view/dataset/601166"}, {"dataset_uid": "601165", "doi": "10.15784/601165", "keywords": "Antarctica; Basal Crevassing; Glacier Hydrology; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; GPR; Radar; Snow/ice; Snow/Ice; Taylor Glacier", "people": "Pettit, Erin", "repository": "USAP-DC", "science_program": null, "title": "Ground Penetrating Radar Data near Blood Falls, Taylor Glacier", "url": "https://www.usap-dc.org/view/dataset/601165"}, {"dataset_uid": "601164", "doi": "10.15784/601164", "keywords": "Antarctica; Basal Crevassing; Glacier Hydrology; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Snow/ice; Snow/Ice", "people": "Pettit, Erin", "repository": "USAP-DC", "science_program": null, "title": "Ablation Stake Data from of Taylor Glacier near Blood Falls", "url": "https://www.usap-dc.org/view/dataset/601164"}, {"dataset_uid": "200028", "doi": "10.7283/FCEN-8050", "keywords": null, "people": null, "repository": "UNAVCO", "science_program": null, "title": "Antarctica Support 2014/2015 - C-528 Blood Falls GPS/GNSS Observations Dataset", "url": "https://www.unavco.org/data/doi/10.7283/fcen-8050"}, {"dataset_uid": "200029", "doi": "10.7914/SN/YW_2013", "keywords": null, "people": null, "repository": "IRIS", "science_program": null, "title": "Blood Falls, McMurdo Dry Va. International Federation of Digital Seismograph Networks. Dataset/Seismic Network", "url": "http://www.fdsn.org/networks/detail/YW_2013/"}], "date_created": "Wed, 28 Nov 2018 00:00:00 GMT", "description": "Recent discoveries of widespread liquid water and microbial ecosystems below the Antarctic ice sheets have generated considerable interest in studying Antarctic subglacial environments. Understanding subglacial hydrology, the persistence of life in extended isolation and the evolution and stability of subglacial habitats requires an integrated, interdisciplinary approach. The collaborative project, Minimally Invasive Direct Glacial Exploration (MIDGE) of the Biogeochemistry, Hydrology and Glaciology of Blood Falls, McMurdo Dry Valleys will integrate geophysical measurements, molecular microbial ecology and geochemical analyses to explore a unique Antarctic subglacial system known as Blood Falls. Blood Falls is a hypersaline, subglacial brine that supports an active microbial community. The subglacial brine is released from a crevasse at the surface of the Taylor Glacier providing an accessible portal into an Antarctic subglacial ecosystem. Recent geochemical and molecular analyses support a marine source for the salts and microorganisms in Blood Falls. The last time marine waters inundated this part of the McMurdo Dry Valleys was during the Late Tertiary, which suggests the brine is ancient. Still, no direct samples have been collected from the subglacial source to Blood Falls and little is known about the origin of this brine or the amount of time it has been sealed below Taylor Glacier. Radar profiles collected near Blood Falls delineate a possible fault in the subglacial substrate that may help explain the localized and episodic nature of brine release. However it remains unclear what triggers the episodic release of brine exclusively at the Blood Falls crevasse or the extent to which the brine is altered as it makes its way to the surface. The MIDGE project aims to determine the mechanism of brine release at Blood Falls, evaluate changes in the geochemistry and the microbial community within the englacial conduit and assess if Blood Falls waters have a distinct impact on the thermal and stress state of Taylor Glacier, one of the most studied polar glaciers in Antarctica. The geophysical study of the glaciological structure and mechanism of brine release will use GPR, GPS, and a small passive seismic network. Together with international collaborators, the \u0027Ice Mole\u0027 team from FH Aachen University of Applied Sciences, Germany (funded by the German Aerospace Center, DLR), MIDGE will develop and deploy innovative, minimally invasive technologies for clean access and brine sample retrieval from deep within the Blood Falls drainage system. These technologies will allow for the collection of samples of the brine away from the surface (up to tens of meters) for geochemical analyses and microbial structure-function experiments. There is concern over the contamination of pristine subglacial environments from chemical and biological materials inherent in the drilling process; and MIDGE will provide data on the efficacy of thermoelectric probes for clean access and retrieval of representative subglacial samples. Antarctic subglacial environments provide an excellent opportunity for researching survivability and adaptability of microbial life and are potential terrestrial analogues for life habitats on icy planetary bodies. The MIDGE project offers a portable, versatile, clean alternative to hot water and mechanical drilling and will enable the exploration of subglacial hydrology and ecosystem function while making significant progress towards developing technologies for minimally invasive and clean sampling of icy systems.", "east": 162.6, "geometry": "POINT(162.2 -77.735)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "NOT APPLICABLE; BACTERIA/ARCHAEA; USAP-DC", "locations": null, "north": -77.7, "nsf_funding_programs": "Antarctic Integrated System Science; Antarctic Integrated System Science; Antarctic Integrated System Science; Antarctic Integrated System Science; Antarctic Instrumentation and Support", "paleo_time": null, "persons": "Tulaczyk, Slawek; Pettit, Erin; Lyons, W. Berry; Mikucki, Jill", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "USAP-DC", "repositories": "IRIS; NCBI GenBank; UNAVCO; USAP-DC", "science_programs": null, "south": -77.77, "title": "Collaborative Research: MIDGE: Minimally Invasive Direct Glacial Exploration of Biogeochemistry, Hydrology and Glaciology of Blood Falls, McMurdo Dry Valleys", "uid": "p0000002", "west": 161.8}, {"awards": "1341701 Bilyk, Kevin", "bounds_geometry": null, "dataset_titles": "Antarctic Ice fish; submission ID #SRP113562", "datasets": [{"dataset_uid": "000206", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Antarctic Ice fish; submission ID #SRP113562", "url": "https://www.ncbi.nlm.nih.gov/genbank/"}], "date_created": "Mon, 31 Jul 2017 00:00:00 GMT", "description": "This work will broaden our knowledge and insights into genetic trait loss or change accompanying species evolution in general as well as within the uniquely isolated and frigid Southern Ocean. The system of oxygen-carrying and related proteins being studied is very important to human health and the two proteins being specifically studied in this work (haptoglobin and hemopexin) have crucial roles in preventing excess iron loading in the kidneys. As such, the project has the potential to contribute novel insights that could be valuable to medical science. The project will also further the NSF goals of training new generations of scientists and of making scientific discoveries available to the general public. The lead principal investigator on the project is an early career scientist whose career development will be enhanced by this project. It will also support the training of several undergraduate students in molecular biology, protein biochemistry, and appreciation of the unique Antarctic fish fauna and environment. The project will contribute to a content-rich web site that will bring to the public the history of biological discoveries and sciences on fishes of the Southern Ocean and through this project the investigators will contribute to an annual polar event at a children\u0027s science museum. The Antarctic icefishes have thrived despite the striking evolutionary loss of the normally indispensable respiratory protein hemoglobin in all species and myoglobin in some. Studies over the past decades have predominately focused on the mechanisms behind hemoprotein losses and the resulting compensatory adaptations in these fish, while evolutionary impact of such losses on the supporting protein genes and functions has remained unaddressed. This project investigates the evolutionary fate of two important partner proteins, the hemoglobin scavenger haptoglobin and the heme scavenger hemopexin (heme groups are the iron-containing functional group of proteins such as hemoglobin and myoglobin). With the permanent hemoglobin-null state in Antarctic icefishes, and particularly in dual hemoglobin- and myoglobin-null species, the preservation of a functional haptoglobin would seem unessential and the role of hemopexin likely diminished. This project seeks to resolve whether co-evolutionary loss or reduction of these supporting proteins occurred with the extinction of the hemoglobin trait in the icefishes, and the molecular mechanisms underlying such changes. The investigators envisage the cold and oxygen rich marine environment as the start of a cascade of relaxation of selection pressures. Initially this would have obviated the need for maintaining functional oxygen carrying proteins, ultimately leading to their permanent loss. These events in turn would have relaxed the maintenance of the network of supporting systems, leading to additional trait loss or change.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Bilyk, Kevin", "platforms": "Not provided", "repo": "NCBI GenBank", "repositories": "NCBI GenBank", "science_programs": null, "south": null, "title": "Evolutionary Fates of Hemoglobin and Heme Scavengers in White-blooded Antarctic Icefishes", "uid": "p0000396", "west": null}, {"awards": "1443554 Buys, Emmanuel", "bounds_geometry": "POLYGON((166.163 -76.665,166.2635 -76.665,166.364 -76.665,166.4645 -76.665,166.565 -76.665,166.6655 -76.665,166.766 -76.665,166.8665 -76.665,166.967 -76.665,167.0675 -76.665,167.168 -76.665,167.168 -76.782,167.168 -76.899,167.168 -77.016,167.168 -77.133,167.168 -77.25,167.168 -77.367,167.168 -77.484,167.168 -77.601,167.168 -77.718,167.168 -77.835,167.0675 -77.835,166.967 -77.835,166.8665 -77.835,166.766 -77.835,166.6655 -77.835,166.565 -77.835,166.4645 -77.835,166.364 -77.835,166.2635 -77.835,166.163 -77.835,166.163 -77.718,166.163 -77.601,166.163 -77.484,166.163 -77.367,166.163 -77.25,166.163 -77.133,166.163 -77.016,166.163 -76.899,166.163 -76.782,166.163 -76.665))", "dataset_titles": "Biosamples and observations from Weddell Seal colonies in McMurdo Sound during the 2015-2016 Antarctic field season", "datasets": [{"dataset_uid": "601028", "doi": "10.15784/601028", "keywords": "Antarctica; Biota; McMurdo Sound; Ross Sea; Sample/collection Description; Sample/Collection Description; Seals", "people": "Buys, Emmanuel; Hindle, Allyson", "repository": "USAP-DC", "science_program": null, "title": "Biosamples and observations from Weddell Seal colonies in McMurdo Sound during the 2015-2016 Antarctic field season", "url": "https://www.usap-dc.org/view/dataset/601028"}], "date_created": "Fri, 26 May 2017 00:00:00 GMT", "description": "The Weddell seal is a champion diving mammal. The physiology that permits these animals to sustain extended breath-hold periods and survive the extreme pressure of diving deep allows them to thrive in icy Antarctic waters. Key elements of their physiological specializations to breath-hold diving are their ability for remarkable adjustment of their heart and blood vessel system, coordinating blood pressure and flow to specific body regions based on their metabolic requirements, and their ability to sustain periods without oxygen. Identifying the details of these strategies has tremendous potential to better inform human medicine, helping us to develop novel therapies for cardiovascular trauma (e.g. stroke, heart attack) and diseases associated with blunted oxygen delivery to tissues (e.g. pneumonia, sepsis, or cancer). The goal of this project is to document specific genes that control these cardiovascular adjustments in seals, and to compare their abundance and activity with humans. Specifically, the investigators will study a signaling pathway that coordinates local blood flow. They will also use tissue samples to generate cultured cells from Weddell seals that can be used to study the molecular effects of low oxygen conditions in the laboratory. The project will further the NSF goals of training new generations of scientists and of making scientific discoveries available to the general public. The project will train a pre-veterinary student researcher will conduct public outreach via a center for community health improvement, a multicultural affairs office, and a public aquarium. The goal of this study is to unravel the molecular mechanisms underlying the dive response. A hallmark of the dive response is tissue-specific vascular system regulation, likely resulting from variation in both nerve inputs and in production of local signaling molecules produced by blood vessel cells. The investigators will use emerging genomic information to begin to unravel the genetics underlying redistribution of the circulation during diving. They will also directly test the hypothesis that modifications in the signaling system prevent local blood vessel changes under low oxygen conditions, thereby allowing the centrally mediated diving reflex to override local physiological responses and to control the constriction of blood vessel walls in Weddell seals. They will perform RNA-sequencing of Weddell seal tissues and use the resulting sequence, along with information from other mammals such as dog, to obtain a full annotation (identifying all genes based on named features of reference genomes) of the existing genome assembly for the Weddell seal, facilitating comparative and species-specific genomic research. They will also generate a Weddell seal pluripotent stem cell line which should be a valuable research tool for cell biologists, molecular biologists and physiologists that will allow them to further test their hypotheses. It is expected that the proposed studies will advance our knowledge of the biochemical and physiological adaptations that allow the Weddell seal to thrive in the Antarctic environment.", "east": 167.168, "geometry": "POINT(166.6655 -77.25)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -76.665, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Buys, Emmanuel; Costa, Daniel; Zapol, Warren; Hindle, Allyson", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -77.835, "title": "Unraveling the Genomic and Molecular Basis of the Dive Response: Nitric Oxide Signaling and Vasoregulation in the Weddell Seal", "uid": "p0000072", "west": 166.163}, {"awards": "1043781 O\u0027Brien, Kristin; 1043576 Crockett, Elizabeth", "bounds_geometry": "POLYGON((-64.45 -63.467,-64.2633 -63.467,-64.0766 -63.467,-63.8899 -63.467,-63.7032 -63.467,-63.5165 -63.467,-63.3298 -63.467,-63.1431 -63.467,-62.9564 -63.467,-62.7697 -63.467,-62.583 -63.467,-62.583 -63.5653,-62.583 -63.6636,-62.583 -63.7619,-62.583 -63.8602,-62.583 -63.9585,-62.583 -64.0568,-62.583 -64.1551,-62.583 -64.2534,-62.583 -64.3517,-62.583 -64.45,-62.7697 -64.45,-62.9564 -64.45,-63.1431 -64.45,-63.3298 -64.45,-63.5165 -64.45,-63.7032 -64.45,-63.8899 -64.45,-64.0766 -64.45,-64.2633 -64.45,-64.45 -64.45,-64.45 -64.3517,-64.45 -64.2534,-64.45 -64.1551,-64.45 -64.0568,-64.45 -63.9585,-64.45 -63.8602,-64.45 -63.7619,-64.45 -63.6636,-64.45 -63.5653,-64.45 -63.467))", "dataset_titles": "Electronic fishing logs; Expedition data of LMG1104; Redox Balance in Antarctic Notothenioid Fishes", "datasets": [{"dataset_uid": "600382", "doi": "10.15784/600382", "keywords": "Antarctica; Antarctic Peninsula; Biota; Fish; Sample/collection Description; Sample/Collection Description; Southern Ocean", "people": "O\u0027Brien, Kristin", "repository": "USAP-DC", "science_program": null, "title": "Redox Balance in Antarctic Notothenioid Fishes", "url": "https://www.usap-dc.org/view/dataset/600382"}, {"dataset_uid": "600390", "doi": "10.15784/600390", "keywords": "Antarctica; Biota; Southern Ocean", "people": "Crockett, Elizabeth", "repository": "USAP-DC", "science_program": null, "title": "Electronic fishing logs", "url": "https://www.usap-dc.org/view/dataset/600390"}, {"dataset_uid": "002687", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG1104", "url": "https://www.rvdata.us/search/cruise/LMG1104"}], "date_created": "Tue, 06 Dec 2016 00:00:00 GMT", "description": "Antarctic channichthyid icefishes are stunning examples of the unique physiological traits that can arise during evolution in a constantly cold environment. Icefishes are the only vertebrates that as adults, lack the circulating oxygen-binding protein hemoglobin (Hb); several species within this family also lack the intracellular oxygen-binding protein myoglobin (Mb) in their heart ventricle. The loss of Hb and Mb has resulted in striking modifications in the cardiovascular system to ensure adequate tissue oxygenation, some of which are energetically costly. Recent indicate there may be at least one benefit to not expressing these heme-centered proteins - oxidized proteins and lipids are higher in red-blooded notothenioids compared to icefishes. The research will address the hypothesis that the loss of Hb and Mb reduces oxidative stress in icefishes compared to red-blooded notothenioid fishes, resulting in a lower rate of protein turnover and energetic cost savings. Specifically, the project will (1) Characterize levels of oxidative stress in red- and white-blooded notothenioid fishes, (2) Determine if red- and white-blooded notothenioids differ in their regulation of iron, (3) Determine if lower levels of oxidized proteins in icefishes result in lower rates of protein turnover and energetic cost savings, and (4) Determine if oxygen-binding proteins promote oxidative stress in-vivo and in-vitro. The results will contribute to the understanding of iron-catalyzed oxidative stress, which is associated with the progression of Alzheimer\u0027s, Parkinson\u0027s and cardiovascular diseases. Moreover, the research will increase understanding of factors related to iron metabolism and oxidative stress in notothenioid fishes that may have played key roles in the success of channichthyid icefishes. The broader impacts include development of a website will enable teachers and students to learn more about the fascinating biology of Antarctic icefishes, as well as the impacts of global climate change and commercial fishing activities on Antarctic fishes. Additionally, Alaska Native high school and undergraduate students will be involved in research at the University of Alaska, Fairbanks.", "east": -62.583, "geometry": "POINT(-63.5165 -63.9585)", "instruments": "EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS", "is_usap_dc": true, "keywords": "Not provided; R/V LMG", "locations": null, "north": -63.467, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Crockett, Elizabeth; O\u0027Brien, Kristin", "platforms": "Not provided; WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "USAP-DC", "repositories": "R2R; USAP-DC", "science_programs": null, "south": -64.45, "title": "Collaborative research: Redox Balance in Antarctic Notothenioid fishes: Do Icefishes have an Advantage?", "uid": "p0000320", "west": -64.45}, {"awards": "0741301 O\u0027Brien, Kristin; 1142720 Crockett, Elizabeth", "bounds_geometry": "POLYGON((-64.45 -63.29,-64.249 -63.29,-64.048 -63.29,-63.847 -63.29,-63.646 -63.29,-63.445 -63.29,-63.244 -63.29,-63.043 -63.29,-62.842 -63.29,-62.641 -63.29,-62.44 -63.29,-62.44 -63.370999999999995,-62.44 -63.452,-62.44 -63.533,-62.44 -63.614,-62.44 -63.69499999999999,-62.44 -63.775999999999996,-62.44 -63.857,-62.44 -63.937999999999995,-62.44 -64.01899999999999,-62.44 -64.1,-62.641 -64.1,-62.842 -64.1,-63.043 -64.1,-63.244 -64.1,-63.445 -64.1,-63.646 -64.1,-63.847 -64.1,-64.048 -64.1,-64.249 -64.1,-64.45 -64.1,-64.45 -64.01899999999999,-64.45 -63.937999999999995,-64.45 -63.857,-64.45 -63.775999999999996,-64.45 -63.69499999999999,-64.45 -63.614,-64.45 -63.533,-64.45 -63.452,-64.45 -63.370999999999995,-64.45 -63.29))", "dataset_titles": "Linkages among Mitochondrial Form, Function and Thermal Tolerance of Antarctic Notothenioid Fishes", "datasets": [{"dataset_uid": "600084", "doi": "10.15784/600084", "keywords": "Biota; Oceans; Pot; Southern Ocean; Trawl", "people": "O\u0027Brien, Kristin", "repository": "USAP-DC", "science_program": null, "title": "Linkages among Mitochondrial Form, Function and Thermal Tolerance of Antarctic Notothenioid Fishes", "url": "https://www.usap-dc.org/view/dataset/600084"}], "date_created": "Sat, 30 Nov 2013 00:00:00 GMT", "description": "Abstract Antarctic notothenioid fishes have evolved in the Southern Ocean for 10-14 MY under an unusual set of circumstances. Their characteristics include the complete absence of the circulating oxygen-binding protein, hemoglobin (Hb) within the Channichthyid (Icefish) family of notothenioids. Moreover, some species within the 16 members of this family have also lost the ability to express the oxygen-binding and storage protein, myoglobin (Mb) in cardiac muscle. Our previous work has determined that the loss of Hb and/or Mb is correlated with significant increases in densities of mitochondria within oxidative tissues, and extensive remodeling of these vital organelles. To date, nothing is known about how modifications in mitochondrial architecture of icefishes affect organelle function, or more importantly, how they affect organismal-level physiology. Most critical for Antarctic fishes is that mitochondrial characteristics have been linked to how well ectotherms can withstand increases in temperature. This collaborative research project will address the hypothesis that the unusual mitochondrial architecture of Antarctic Channichthyids has led to changes in function that impact their ability to withstand elevations in temperature. Specifically, the research will (1) determine if the unusual mitochondrial architecture of icefishes affects function and contributes to organismal thermal sensitivity, (2) identify differences in organismal thermal tolerance between red- and white- blooded notothenioids, (3) identify molecular mechanisms regulating changes in mitochondrial structure in icefishes. The results may establish channichthyid icefishes as a sentinel taxon for signaling the impact of global warming on the Southern Ocean. Broad impacts of this project will be realized by participation of high school biology teachers in field work through cooperation with the ARMADA project at the University of Rhode Island, as well as graduate education.", "east": -62.44, "geometry": "POINT(-63.445 -63.695)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -63.29, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Crockett, Elizabeth; O\u0027Brien, Kristin", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -64.1, "title": "Collaborative Research: Linkages among Mitochondrial Form, Function and Thermal Tolerance of Antarctic Notothenioid Fishes", "uid": "p0000483", "west": -64.45}, {"awards": "0636696 DeVries, Arthur", "bounds_geometry": "POLYGON((-68.0025 -52.7599,-67.07254 -52.7599,-66.14258 -52.7599,-65.21262 -52.7599,-64.28266 -52.7599,-63.3527 -52.7599,-62.42274 -52.7599,-61.49278 -52.7599,-60.56282 -52.7599,-59.63286 -52.7599,-58.7029 -52.7599,-58.7029 -53.98242,-58.7029 -55.20494,-58.7029 -56.42746,-58.7029 -57.64998,-58.7029 -58.8725,-58.7029 -60.09502,-58.7029 -61.31754,-58.7029 -62.54006,-58.7029 -63.76258,-58.7029 -64.9851,-59.63286 -64.9851,-60.56282 -64.9851,-61.49278 -64.9851,-62.42274 -64.9851,-63.3527 -64.9851,-64.28266 -64.9851,-65.21262 -64.9851,-66.14258 -64.9851,-67.07254 -64.9851,-68.0025 -64.9851,-68.0025 -63.76258,-68.0025 -62.54006,-68.0025 -61.31754,-68.0025 -60.09502,-68.0025 -58.8725,-68.0025 -57.64998,-68.0025 -56.42746,-68.0025 -55.20494,-68.0025 -53.98242,-68.0025 -52.7599))", "dataset_titles": "Expedition Data; Expedition data of LMG0809; Metadata associated with the description of Akarotaxis gouldae n. sp. (Bathydraconidae)", "datasets": [{"dataset_uid": "601811", "doi": null, "keywords": "Antarctica; Bellingshausen Sea; Cryosphere; Southern Ocean", "people": "Biesack, Ellen; Corso, Andrew; Desvignes, Thomas; McDowell, Jan; Cheng, Chi-Hing; Steinberg, Deborah; Hilton, Eric", "repository": "USAP-DC", "science_program": "LTER", "title": "Metadata associated with the description of Akarotaxis gouldae n. sp. (Bathydraconidae)", "url": "https://www.usap-dc.org/view/dataset/601811"}, {"dataset_uid": "002728", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0809", "url": "https://www.rvdata.us/search/cruise/LMG0809"}, {"dataset_uid": "001493", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0810"}, {"dataset_uid": "001504", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0809"}], "date_created": "Tue, 04 May 2010 00:00:00 GMT", "description": "Antarctic notothenioid fish evolved antifreeze (AF) proteins that prevent ice crystals that enter their body fluids from growing, and thereby avoid freezing in their icy habitats. However, even in the extreme cold Antarctic marine environment, regional gradations of severity are found. The biological correlate for environmental severity in fish is the endogenous ice load, which likely determines the tolerable limit of environmental severity for notothenioid habitation. The endogenous ice load develops from environmental ice crystals entering through body surfaces and somehow localizing to the spleen. How prone the surface tissues are to ice entry, how ice reaches the spleen, and what the fate of splenic ice is, requires elucidation. Spleen sequestration of ice raises the hypothesis that macrophages may play a role in the translocation and perhaps elimination of AF-bound ice crystals. Antifreeze glycoproteins (AFGP) act in concert with a second, recently discovered antifreeze called antifreeze potentiating protein (AFPP), necessitating an assessment of the contribution of AFPP to freezing avoidance. Recent research suggests that the exocrine pancreas and the anterior stomach, not the liver, synthesize AFGPs and secrete them into the intestine, from where they may be returned to the blood. A GI-to-blood transport is a highly unconventional path for a major plasma protein and also begs the questions, What is the source of blood AFPP?. Why are two distinct AF proteins needed and what is the chronology of their evolution? What genomic changes in the DNA are associated with the development or loss of the antifreeze trait? Experiments described in this proposal address these interrelated questions of environmental, organismal, and evolutionary physiology, and will further our understanding of novel vertebrate physiologies, the limits of environmental adaptation, and climatically driven changes in the genome. The proposed research will (1) determine the temporal and spatial heterogeneity of environmental temperature and iciness in progressively more severe fish habitats in the greater McMurdo Sound area, and in the milder Arthur Harbor at Palmer Station. The splenic ice load in fishes inhabiting these sites will be determined to correlate to environmental severity and habitability. (2) Assess the surface tissue site of ice entry and their relative barrier properties in intact fish and isolated tissues preparations (3) Assess the role of immune cells in the fate of endogenous ice, (4) determine whether the blood AFGPs are from intestinal/rectal uptake, (5) examine the contribution of AFPP to the total blood AF activity (6) evaluate the progression of genomic changes in the AFGP locus across Notothenioidei as modulated by disparate thermal environments, in four selected species through the analyses of large insert DNA BAC clones. The origin and evolution of AFPP will be examined also by analyzing BAC clones encompassing the AFPP genomic locus. The broader impacts of the proposed research include training of graduate and undergraduate students in research approaches ranging from physical field measurements to cutting edge genomics. Undergraduate research projects have lead to co-authored publications and will continue to do so. Outreach includes establishing Wiki websites on topics of Antarctic fish biology and freeze avoidance, providing advisory services to the San Francisco Science Exploratorium, and making BAC libraries available to interested polar biologists. This research theme has repeatedly received national and international science news coverage and will continue to be disseminated to the public in that manner.", "east": -58.7029, "geometry": "POINT(-63.3527 -58.8725)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ADCP", "is_usap_dc": false, "keywords": "R/V LMG", "locations": null, "north": -52.7599, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Devries, Arthur", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "USAP-DC", "repositories": "R2R; USAP-DC", "science_programs": null, "south": -64.9851, "title": "Environmental, Organismal and Evolutionary Physiology of Freeze Avoidance in Antarctic Notothenioid Fishes", "uid": "p0000560", "west": -68.0025}, {"awards": "0089451 Detrich, H. William", "bounds_geometry": "POLYGON((-70.907 -52.353,-69.8619 -52.353,-68.8168 -52.353,-67.7717 -52.353,-66.7266 -52.353,-65.6815 -52.353,-64.6364 -52.353,-63.5913 -52.353,-62.5462 -52.353,-61.5011 -52.353,-60.456 -52.353,-60.456 -53.64334,-60.456 -54.93368,-60.456 -56.22402,-60.456 -57.51436,-60.456 -58.8047,-60.456 -60.09504,-60.456 -61.38538,-60.456 -62.67572,-60.456 -63.96606,-60.456 -65.2564,-61.5011 -65.2564,-62.5462 -65.2564,-63.5913 -65.2564,-64.6364 -65.2564,-65.6815 -65.2564,-66.7266 -65.2564,-67.7717 -65.2564,-68.8168 -65.2564,-69.8619 -65.2564,-70.907 -65.2564,-70.907 -63.96606,-70.907 -62.67572,-70.907 -61.38538,-70.907 -60.09504,-70.907 -58.8047,-70.907 -57.51436,-70.907 -56.22402,-70.907 -54.93368,-70.907 -53.64334,-70.907 -52.353))", "dataset_titles": "Expedition Data; Expedition data of LMG0304A", "datasets": [{"dataset_uid": "002707", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0304A", "url": "https://www.rvdata.us/search/cruise/LMG0304A"}, {"dataset_uid": "001869", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0105"}, {"dataset_uid": "001704", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0304"}], "date_created": "Tue, 04 May 2010 00:00:00 GMT", "description": "Abstract\u003cbr/\u003eOPP-0089451\u003cbr/\u003eP.I. William Detrich\u003cbr/\u003e\u003cbr/\u003e As the Southern Ocean cooled during the past 25 million years, the fishes of Antarctic coastal waters evolved biochemical and physiological adaptations that maintain essential cellular processes such as cytoskeletal function and gene transcription. Their microtubules, for example, assemble and function at body temperatures (-1.8 to +1 oC) well below those of homeotherms and temperate poikilotherms. The long range goals of the proposed research are to determine, at the molecular level, the adaptations that enhance the assembly of microtubules, the function of kinesin motors, and the expression of globin and tubulin genes. The specific objectives are three: 1) to determine the primary sequence changes and posttranslational modifications that contribute to the efficient polymerization of Antarctic fish tubulins at low temperatures; 2) to evaluate the biochemical adaptations required for efficient function of the brain kinesin motor of Antarctic fishes at low temperatures; and 3) to characterize the structure, organization, and promoter-driven expression of globin and tubulin genes from an Antarctic rockcod (Notothenia coriiceps) and a temperate congener (N. angustata). Brain tubulins from Antarctic fishes differ from those of temperate and warm-blooded vertebrates both in unusual primary sequence substitutions (located primarily in lateral loops and the cores of tubulin monomers) and in posttranslational C-terminal glutamylation. Potential primary sequence adaptations of the Antarctic fish tubulins will be tested directly by production of wild-type and site directed tubulin mutants for functional analysis in vitro. The capacity of mutated and wild-type fish tubulins to form \"cold-stable\" microtubules will be determined by measurement of their critical concentrations for assembly and by analysis of their dynamics by video-enhanced microscopy. Three unusual substitutions in the kinesin motor domain of Chionodraco rastrospinosus may enhance mechanochemical activity at low temperature by modifying the binding of ATP and/or the velocity of the motor. To test the functional significance of these changes, the fish residues will be converted individually, and in concert, to those found in mammalian brain kinesin. Reciprocal substitutions will be introduced into the framework of the mammalian kinesin motor domain. After production in Escherichia coli and purification, the functional performance of the mutant motor domains will be evaluated by measurement of the temperature dependence of their ATPase and motility activities. Molecular adaptation of gene expression in N. coriiceps will be analyzed using an a-globin/b-globin gene pair and an a-tubulin gene cluster. Structural features of N. coriiceps globin and tubulin gene regulatory sequences (promoters and enhancers) that support efficient expression will be assessed by transient transfection assay of promoter/luciferase reporter plasmid constructs in inducible erythrocytic and neuronal model cell systems followed by assay of luciferase reporter activity. Together, these studies should reveal the molecular adaptations of Antarctic fishes that maintain efficient cytoskeletal assembly, mechanochemical motor function, and gene expression at low temperatures. In the broadest sense, this research program should advance the molecular understanding of the poikilothermic mode of life.", "east": -60.456, "geometry": "POINT(-65.6815 -58.8047)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; IN SITU/LABORATORY INSTRUMENTS \u003e PRESSURE/HEIGHT METERS \u003e PRESSURE SENSORS; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ADCP", "is_usap_dc": false, "keywords": "R/V LMG", "locations": null, "north": -52.353, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Sidell, Bruce; Detrich, H. William", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "R2R", "repositories": "R2R", "science_programs": null, "south": -65.2564, "title": "Structure, Function, and Expression of Tubulins, Globins, and Microtubule-Dependent Motors from Cold-Adapted Antarctic Fishes", "uid": "p0000591", "west": -70.907}, {"awards": "0538594 Ponganis, Paul", "bounds_geometry": "POLYGON((165.983 -77.683,166.0164 -77.683,166.0498 -77.683,166.0832 -77.683,166.1166 -77.683,166.15 -77.683,166.1834 -77.683,166.2168 -77.683,166.2502 -77.683,166.2836 -77.683,166.317 -77.683,166.317 -77.6897,166.317 -77.6964,166.317 -77.7031,166.317 -77.7098,166.317 -77.7165,166.317 -77.7232,166.317 -77.7299,166.317 -77.7366,166.317 -77.7433,166.317 -77.75,166.2836 -77.75,166.2502 -77.75,166.2168 -77.75,166.1834 -77.75,166.15 -77.75,166.1166 -77.75,166.0832 -77.75,166.0498 -77.75,166.0164 -77.75,165.983 -77.75,165.983 -77.7433,165.983 -77.7366,165.983 -77.7299,165.983 -77.7232,165.983 -77.7165,165.983 -77.7098,165.983 -77.7031,165.983 -77.6964,165.983 -77.6897,165.983 -77.683))", "dataset_titles": "The Aerobic Dive Limit: Oxygen Transport and Depletion in Emperor Penguins", "datasets": [{"dataset_uid": "600057", "doi": "10.15784/600057", "keywords": "Antarctica; Biota; Oceans; Penguin; Southern Ocean", "people": "Ponganis, Paul", "repository": "USAP-DC", "science_program": null, "title": "The Aerobic Dive Limit: Oxygen Transport and Depletion in Emperor Penguins", "url": "https://www.usap-dc.org/view/dataset/600057"}], "date_created": "Sun, 20 Dec 2009 00:00:00 GMT", "description": "The research will examine blood and muscle oxygen store depletion in relation to the documented aerobic dive limit (ADL, onset of post-dive blood lactate accumulation) in diving of emperor penguins. The intellectual merits of this proposal involve its evaluation of the physiological basis of the ADL concept. The ADL is probably the most commonly-used, but rarely measured, factor to interpret and model the behavior and foraging ecology of diving animals. Based on prior studies, and on recent investigations of respiratory and blood oxygen depletion during dives of emperor penguins, it is hypothesized that the ADL is a result of the depletion of myoglobin (Mb)-bound oxygen and increased glycolysis in the primary locomotory muscles. This project will accurately define the physiological mechanisms underlying the ADL through 1) evaluation of the rate and magnitude of muscle oxygen depletion during dives in relation to the previously measured ADL, 2) characterization of the hemoglobin-oxygen dissociation curve in blood of emperor penguins and comparison of that curve to those of other diving and non-diving species, 3) application of the emperor hemoglogin-oxygen dissociation curve to previously collected oxygen and hemoglobin data in order to estimate the rate and magnitude of blood oxygen depletion during dives, and 4) measurement of muscle phosphoocreatine and glycogen concentrations in order to estimate their potential contributions to muscle energy metabolism during diving. The project also continues the census and monitoring of the emperor colonies in the Ross Sea, which is especially important in light of both fisheries activity and the movement of iceberg B15-A. Broader impacts of the project include: 1) technological development of microprocessor-based, \"backpack\" near-infrared spectrophotometer, which will be applicable not only to other species, but also to other fields (i.e., exercise physiology), 2) collaboration with the Department of Anesthesia at the U.S. Naval Hospital in San Diego in the training of anesthesia residents in research techniques, 3) the training and thesis research of two graduate students in these techniques and in Antarctic field research, and 4) a better understanding of the ADL concept and its use in the fields of diving behavior and physiology. In addition the annual census of emperor penguin colonies in the Ross Sea, in conjunction with the continued evaluation of previously developed remote cameras to monitor colony status, will form the basis of a new educational web site, and allow development of an educational outreach program to school children through SeaWorld of San Diego.", "east": 166.317, "geometry": "POINT(166.15 -77.7165)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -77.683, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Ponganis, Paul", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -77.75, "title": "The Aerobic Dive Limit: Oxygen Transport and Depletion in Emperor Penguins", "uid": "p0000535", "west": 165.983}, {"awards": "0437887 Sidell, Bruce", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "Differential Expression of Oxygen-binding Proteins in Antarctic Fishes Affects Nitric Oxide-mediated Pathways of Angiogenesis and Mitochondrial Biogenesis; Expedition Data; Expedition data of LMG0705; Expedition data of LMG0706", "datasets": [{"dataset_uid": "001534", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0705"}, {"dataset_uid": "002712", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0705", "url": "https://www.rvdata.us/search/cruise/LMG0705"}, {"dataset_uid": "002713", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0706", "url": "https://www.rvdata.us/search/cruise/LMG0706"}, {"dataset_uid": "600039", "doi": "10.15784/600039", "keywords": "Biota; Oceans; Pot; Sample/collection Description; Sample/Collection Description; Southern Ocean; Trawl", "people": "Sidell, Bruce", "repository": "USAP-DC", "science_program": null, "title": "Differential Expression of Oxygen-binding Proteins in Antarctic Fishes Affects Nitric Oxide-mediated Pathways of Angiogenesis and Mitochondrial Biogenesis", "url": "https://www.usap-dc.org/view/dataset/600039"}], "date_created": "Sun, 06 Dec 2009 00:00:00 GMT", "description": "The polar ocean presently surrounding Antarctica is the coldest, most thermally stable marine environment on earth. Because oxygen solubility in seawater is inversely proportional to temperature, the cold Antarctic seas are an exceptionally oxygen-rich aquatic habitat. Eight families of a single perciform suborder, the Notothenioidei, dominate the present fish fauna surrounding Antarctica. Notothenioids account for approximately 35% of fish species and 90% of fish biomass south of the Antarctic Polar Front. Radiation of closely related notothenioid species thus has occurred rapidly and under a very unusual set of conditions: relative oceanographic isolation from other faunas due to circumpolar currents and deep ocean trenches surrounding the continent, chronically, severely cold water temperatures, very high oxygen availability, very low levels of niche competition in a Southern Ocean depauperate of species subsequent to a dramatic crash in species diversity of fishes that occurred sometime between the mid-Tertiary and present. These features make Antarctic notothenioid fishes an uniquely attractive group for the study of physiological and biochemical adaptations to cold body temperature. \u003cbr/\u003eFew distinctive features of Antarctic fishes are as unique as the pattern of expression of oxygen-binding proteins in one notothenioid family, the Channichthyidae (Antarctic icefishes). All channichthyid icefishes lack the circulating oxygen-binding protein, hemoglobin (Hb); the intracellular oxygen-binding protein, myoglobin (Mb) is not uniformly expressed in species of this family. Both proteins are normally considered essential for adequate delivery of oxygen to aerobically poised tissues of animals. To compensate for the absence of Hb, icefishes have developed large hearts, rapidly circulate a large blood volume and possess elaborate vasculature of larger lumenal diameter than is seen in red-blooded fishes. Loss of Mb expression in oxidative muscles correlates with dramatic elevation in density of mitochondria within the cell, although each individual organelle is less densely packed with respiratory proteins. \u003cbr/\u003eWithin the framework of oxygen movement, the adaptive significance of greater vascular density and mitochondrial populations is understandable but mechanisms underlying development of these characteristics remain unknown. The answer may lie in another major function of both Hb and Mb, degradation of the ubiquitous bioactive compound, nitric oxide (NO). The research will test the hypothesis that loss of hemoprotein expression in icefishes has resulted in an increase in levels of NO that mediate modification of vascular systems and expansion of mitochondrial populations in oxidative tissues. The objectives of the proposal are to quantify the vascular density of retinas in +Hb and -Hb notothenioid species, to characterize NOS isoforms and catalytic activity in retina and cardiac muscle of Antarctic notothenioid fishes, to evaluate level of expression of downstream factors implicated in angiogenesis (in retinal tissue) and mitochondrial biogenesis (in cardiac muscle), and to determine whether inhibition of NOS in vivo results in regression of angiogenic and mitochondrial biogenic responses in icefishes. Broader impacts range from basic biology, through training of young scientists, to enhanced understanding of clinically relevant biomedical processes.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ADCP; IN SITU/LABORATORY INSTRUMENTS \u003e PRESSURE/HEIGHT METERS \u003e PRESSURE SENSORS; IN SITU/LABORATORY INSTRUMENTS \u003e PHOTON/OPTICAL DETECTORS \u003e TURBIDITY METERS", "is_usap_dc": true, "keywords": "R/V LMG; Not provided", "locations": null, "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Sidell, Bruce", "platforms": "Not provided; WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "R2R", "repositories": "R2R; USAP-DC", "science_programs": null, "south": -90.0, "title": "Collaborative Research: Differential Expression of Oxygen-binding Proteins in Antarctic Fishes Affects Nitric Oxide-mediated Pathways of Angiogenesis and Mitochondrial Biogenesis.", "uid": "p0000527", "west": -180.0}, {"awards": "0229638 Ponganis, Paul", "bounds_geometry": "POLYGON((163 -77,163.4 -77,163.8 -77,164.2 -77,164.6 -77,165 -77,165.4 -77,165.8 -77,166.2 -77,166.6 -77,167 -77,167 -77.1,167 -77.2,167 -77.3,167 -77.4,167 -77.5,167 -77.6,167 -77.7,167 -77.8,167 -77.9,167 -78,166.6 -78,166.2 -78,165.8 -78,165.4 -78,165 -78,164.6 -78,164.2 -78,163.8 -78,163.4 -78,163 -78,163 -77.9,163 -77.8,163 -77.7,163 -77.6,163 -77.5,163 -77.4,163 -77.3,163 -77.2,163 -77.1,163 -77))", "dataset_titles": "Diving Physiology and Behavior of Emperor Penguins", "datasets": [{"dataset_uid": "600031", "doi": "10.15784/600031", "keywords": "Antarctica; Biota; Oceans; Penguin; Southern Ocean", "people": "Ponganis, Paul", "repository": "USAP-DC", "science_program": null, "title": "Diving Physiology and Behavior of Emperor Penguins", "url": "https://www.usap-dc.org/view/dataset/600031"}], "date_created": "Mon, 31 Mar 2008 00:00:00 GMT", "description": "The emperor penguin, Aptenodytes forsteri, is the premier avian diver and a top predator in the Antarctic ecosystem. The routine occurrence of 500-m diver during foraging trips to sea is both a physiological and behavior enigma. The objectives of this project address how and why emperors dive as deep and long as they do. The project examines four major topics in the diving biology of emperor penguins: pressure tolerance, oxygen store management, end-organ tolerance of diving hypoxemia/ischemia, and deep-dive foraging behavior. These subjects are relevant to the role of the emperor as a top predator in the Antarctic ecosystem, and to critical concepts in diving physiology, including decompression sickness, nitrogen narcosis, shallow water blackout, hypoxemic tolerance, and extension of aerobic dive time. The following hypotheses will be tested: 1) Prevention of nitrogen narcosis and decompression sickness in emperor penguins is achieved by inhibition of pulmonary gas exchange at depth. 2) Shallow water black out does not occur because of greater cerebral hypoxemic tolerance, and, in deep dives, because of resumption of pulmonary gas exchange during final ascent. 3) The rate of depletion of the blood oxygen store is a function of depth of dive and heart rate. 4) The aerobic dive limit (ADL) reflects the onset of lactate accumulation in locomotory muscle, not total depletion of all oxygen stores. 5) Elevation of tissue antioxidant capacity and free-radical scavenging enzyme activities protect against the routine ischemia/reperfusion which occur during diving. 6) During deep dives, the Antarctic silverfish, Pleuorogramma antarcticum, is the primary prey item for emperors. \u003cbr/\u003e\u003cbr/\u003eIn addition to evaluation of the hypotheses below, the project has broader impacts in several areas such as partnership with foreign and national institutes and organizations (e.g., the National Institute of Polar Research of Japan, Centro de Investigacioines del Noroeste of Mexico, National Geographic, the University of Texas Southwestern Medical Center, and Sea World). Participation in National Geographic television documentaries will provide unique educational opportunities for the general public; development of state-of-the-art technology (e.g., blood oxygen electrode recorders, blood samplers, and miniaturized digital cameras) will lay the groundwork for future research by this group and others; and the effects of the B15 iceberg on breeding success of emperor penguins will continue to be evaluated with population censuses during planned fieldwork at several Ross Sea emperor penguin colonies.", "east": 167.0, "geometry": "POINT(165 -77.5)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -77.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Ponganis, Paul", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -78.0, "title": "Diving Physiology and Behavior of Emperor Penguins", "uid": "p0000239", "west": 163.0}]

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