USAP-DC

{"dp_type": "Project", "free_text": "Notothenioidei"}

[{"awards": "1341661 Near, Thomas", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "Phylogenomics of Antarctic notothenioid fishes", "datasets": [{"dataset_uid": "601264", "doi": null, "keywords": "Adaptive Radiation; Antarctica; Fish; Notothenioidei; Phylogeny; Southern Ocean; Speciation", "people": "Near, Thomas; Dornburg, Alex", "repository": "USAP-DC", "science_program": null, "title": "Phylogenomics of Antarctic notothenioid fishes", "url": "https://www.usap-dc.org/view/dataset/601264"}, {"dataset_uid": "601262", "doi": "10.15784/601262", "keywords": "Adaptive Radiation; Antarctica; Fish; Notothenioidei; Phylogeny; Southern Ocean; Speciation", "people": "Dornburg, Alex; Near, Thomas", "repository": "USAP-DC", "science_program": null, "title": "Phylogenomics of Antarctic notothenioid fishes", "url": "https://www.usap-dc.org/view/dataset/601262"}], "date_created": "Sat, 29 Feb 2020 00:00:00 GMT", "description": "Understanding how groups of organisms respond to climate change is fundamentally important to assessing the impacts of human activities as well as understanding how past climatic shifts have shaped biological diversity over deep stretches of time. The fishes occupying the near-shore marine habitats around Antarctica are dominated by one group of closely related species called notothenioids. It appears dramatic changes in Antarctic climate were important in the origin and evolutionary diversification of this economically important lineage of fishes. Deposits of fossil fishes in Antarctica that were formed when the continent was experiencing milder temperatures show that the area was home to a much more diverse array of fish lineages. Today the waters of the Southern Ocean are very cold, and often below freezing, but notothenioids fishes exhibit a number of adaptions to live in this harsh set of marine habitats, including the presence of anti-freeze proteins. This research project will collect DNA sequences from hundreds of genes to infer the genealogical relationships of nearly all 124 notothenioid species, and use mathematical techniques to estimate the ages of species and lineages. Knowledge on the timing of evolutionary divergence in notothenioids will allow investigators to assess if timing of previous major climatic shifts in Antarctica are correlated with key events in the formation of the modern Southern Ocean fish fauna. The project will also further the NSF goals of making scientific discoveries available to the general public and of training new generations of scientists. The project will support educational outreach activities to teenager groups and to the general public through a natural history museum exhibit and other public lectures. It will provide professional training opportunities for graduate students and a postdoctoral research scholar. \u003cbr/\u003e\u003cbr/\u003eAdaptive radiation, where lineages experience high rates of evolutionary diversification coincident with ecological divergence, is mostly studied in island ecosystems. Notothenioids dominate the fish fauna of the Southern Ocean and exhibit antifreeze glycoproteins that allow occupation of the subzero waters. Notothenioids are noted as one of the only examples of adaptive radiation among marine fishes, but the evolutionary history of diversification and radiation into different ecological habitats is poorly understood. This research will generate a species phylogeny (evolutionary history) for nearly all of the 124 recognized notothenioid species to investigate the mechanisms of adaptive radiation in this lineage. The phylogeny is inferred from approximately 350 genes sampled using next generation DNA sequencing and related techniques. Morphometric data are taken for museum specimens to investigate the tempo of morphological diversification and to determine if there are correlations between rates of lineage diversification and the origin of morphological disparity. The patterns of lineage, morphological, and ecological diversification in the notothenioid radiation will be compared to the paleoclimatic record to determine if past instances of global climate change have shaped the evolutionary diversification of this lineage of polar-adapted fishes.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "FISH; Fish; AMD; USA/NSF; Southern Ocean; Amd/Us; NOT APPLICABLE; USAP-DC; MARINE ECOSYSTEMS; Notothenioidei; Phylogeny", "locations": "Southern Ocean", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Near, Thomas", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -90.0, "title": "Phylogenomic Study of Adaptive Radiation in Antarctic Fishes", "uid": "p0010087", "west": -180.0}, {"awards": "1341602 Crockett, Elizabeth; 1341663 O\u0027Brien, Kristin", "bounds_geometry": null, "dataset_titles": "Acclimation of cardiovascular function in Notothenia coriiceps; Adrenergic and adenosinergic regulation of the cardiovascular system in the Antarctic icefish Chaenocephalus aceratus; Cardiac metabolism in Antarctic fishes in response to an acute increase in temperature; Chaenocephalus aceratus HIF-1A mRNA, complete cds; Chionodraco rastrospinosus HIF-1A mRNA, partial cds; Effects of acute warming on cardiovascular performance of Antarctic fishes; Eleginops maclovinus HIF-1A mRNA, partial cds; Gymnodraco acuticeps HIF-1A mRNA, partial cds; Hypoxia response of hearts of Antarctic fishes; Maximum cardiac performance of Antarctic fishes that lack haemoglobin and myoglobin: exploring the effect of warming on nature\u2019s natural knockouts; Measurements of splenic contraction in Antarctic fishes; Mitochondrial membranes in cardiac muscle from Antarctic notothenioid fishes vary in phospholipid composition and membrane fluidity; Notothenia coriiceps HIF-1A mRNA, complete cds; Parachaenichthys charcoti HIF-1A mRNA, partial cds; Physical, chemical, and functional properties of neuronal membranes vary between species of Antarctic notothenioids differing in thermal tolerance; Thermal sensitivity of membrane fluidity and integrity in hearts of Antarctic fishes that vary in expression of hemoglobin and myoglobin", "datasets": [{"dataset_uid": "601407", "doi": "10.15784/601407", "keywords": "Antarctica; Antarctic Peninsula", "people": "Axelsson, Michael; O\u0027Brien, Kristin; Joyce, William", "repository": "USAP-DC", "science_program": null, "title": "Measurements of splenic contraction in Antarctic fishes", "url": "https://www.usap-dc.org/view/dataset/601407"}, {"dataset_uid": "200184", "doi": "10.5061/dryad.83vc5", "keywords": null, "people": null, "repository": "Dryad", "science_program": null, "title": "Maximum cardiac performance of Antarctic fishes that lack haemoglobin and myoglobin: exploring the effect of warming on nature\u2019s natural knockouts", "url": "https://doi.org/10.5061/dryad.83vc5"}, {"dataset_uid": "200185", "doi": "10.5061/dryad.k90h35k", "keywords": null, "people": null, "repository": "Dryad", "science_program": null, "title": "Mitochondrial membranes in cardiac muscle from Antarctic notothenioid fishes vary in phospholipid composition and membrane fluidity", "url": "https://doi.org/10.5061/dryad.k90h35k"}, {"dataset_uid": "200186", "doi": "10.5061/dryad.qm0b25h", "keywords": null, "people": null, "repository": "Dryad", "science_program": null, "title": "Physical, chemical, and functional properties of neuronal membranes vary between species of Antarctic notothenioids differing in thermal tolerance", "url": "https://doi.org/10.5061/dryad.qm0b25h"}, {"dataset_uid": "200187", "doi": null, "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "Chaenocephalus aceratus HIF-1A mRNA, complete cds", "url": "https://www.ncbi.nlm.nih.gov/nuccore/KX950828"}, {"dataset_uid": "200188", "doi": null, "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "Notothenia coriiceps HIF-1A mRNA, complete cds", "url": "https://www.ncbi.nlm.nih.gov/nuccore/KX950829"}, {"dataset_uid": "200189", "doi": null, "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "Eleginops maclovinus HIF-1A mRNA, partial cds", "url": "https://www.ncbi.nlm.nih.gov/nuccore/KX950830"}, {"dataset_uid": "601405", "doi": "10.15784/601405", "keywords": "Antarctica; Antarctic Peninsula", "people": "O\u0027Brien, Kristin", "repository": "USAP-DC", "science_program": null, "title": "Cardiac metabolism in Antarctic fishes in response to an acute increase in temperature", "url": "https://www.usap-dc.org/view/dataset/601405"}, {"dataset_uid": "601406", "doi": "10.15784/601406", "keywords": "Antarctica; Antarctic Peninsula", "people": "O\u0027Brien, Kristin", "repository": "USAP-DC", "science_program": null, "title": "Hypoxia response of hearts of Antarctic fishes", "url": "https://www.usap-dc.org/view/dataset/601406"}, {"dataset_uid": "601408", "doi": "10.15784/601408", "keywords": "Antarctica; Antarctic Peninsula", "people": "O\u0027Brien, Kristin; Axelsson, Michael; Crockett, Elizabeth; Joyce, William; Farrell, Anthony; Egginton, Stuart", "repository": "USAP-DC", "science_program": null, "title": "Acclimation of cardiovascular function in Notothenia coriiceps", "url": "https://www.usap-dc.org/view/dataset/601408"}, {"dataset_uid": "601409", "doi": "10.15784/601409", "keywords": "Antarctica; Antarctic Peninsula", "people": "Axelsson, Michael; O\u0027Brien, Kristin; Egginton, Stuart; Joyce, Michael; Farrell, Anthony", "repository": "USAP-DC", "science_program": null, "title": "Adrenergic and adenosinergic regulation of the cardiovascular system in the Antarctic icefish Chaenocephalus aceratus", "url": "https://www.usap-dc.org/view/dataset/601409"}, {"dataset_uid": "601410", "doi": "10.15784/601410", "keywords": "Antarctica; Antarctic Peninsula; Biota; Fish", "people": "Crockett, Elizabeth; Egginton, Stuart; Axelsson, Michael; Farrell, Anthony; Joyce, William; O\u0027Brien, Kristin", "repository": "USAP-DC", "science_program": null, "title": "Effects of acute warming on cardiovascular performance of Antarctic fishes", "url": "https://www.usap-dc.org/view/dataset/601410"}, {"dataset_uid": "601414", "doi": "10.15784/601414", "keywords": "Antarctica; Antarctic Peninsula", "people": "Farnoud, Amir; Evans, Elizabeth; Crockett, Elizabeth; O\u0027Brien, Kristin", "repository": "USAP-DC", "science_program": null, "title": "Thermal sensitivity of membrane fluidity and integrity in hearts of Antarctic fishes that vary in expression of hemoglobin and myoglobin", "url": "https://www.usap-dc.org/view/dataset/601414"}, {"dataset_uid": "200190", "doi": null, "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "Parachaenichthys charcoti HIF-1A mRNA, partial cds", "url": "https://www.ncbi.nlm.nih.gov/nuccore/KX950833"}, {"dataset_uid": "200191", "doi": null, "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "Gymnodraco acuticeps HIF-1A mRNA, partial cds", "url": "https://www.ncbi.nlm.nih.gov/nuccore/kx950832"}, {"dataset_uid": "200192", "doi": null, "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "Chionodraco rastrospinosus HIF-1A mRNA, partial cds", "url": "https://www.ncbi.nlm.nih.gov/nuccore/kx950831"}], "date_created": "Wed, 26 Feb 2020 00:00:00 GMT", "description": "The ocean surrounding Antarctica is home to an extraordinary assemblage of fishes, dominated by a single group that are extremely well-suited to life in icy waters and which are of significant ecological importance there. Of great concern is the capacity of these fishes to withstand increases in temperature as the region of the Western Antarctic Peninsula warms at a rate faster than any other area in the Southern hemisphere. One particular group of Antarctic fishes, known as the icefishes, are particularly vulnerable to increases in temperature because unlike all other vertebrates on earth, icefishes are white-blooded due to their lack of the oxygen-binding protein hemoglobin. This greatly reduces their capacity to transport and deliver oxygen to tissues compared to red-blooded Antarctic fishes. Previous studies have shown that icefishes are indeed less tolerant to elevations in temperature but the underlying factors are completely unknown. Additionally, it is not understood if red- or white-blooded Antarctic fishes can adjust, or acclimate, to modest increases in temperature, similar to those changes in temperature the animals might experience as the earth warms. The investigators will determine if heart function and/or nervous system function limits thermal tolerance of Antarctic fishes, and will determine their capacity to acclimate to warmer temperatures. The project will further the NSF goal of training new generations of scientists by training graduate and undergraduate students. In addition, the project will collaborate with a high school biology teacher from a school which serves a largely minority student body. The students will learn about the marine environment, and will construct a camera to be used in the field to learn more about Antarctic fishes. Two students and the teacher will also attend a summer marine biology internship program.\u003cbr/\u003e\u003cbr/\u003eAntarctic fishes within the suborder Notothenioidei (called \"notothenioids\") are among the organisms on earth least able to deal with changes in temperature. The hemoglobinless icefish are even less able to withstand temperature changes than are red-blooded notothenioids. While this is well documented, the underlying physiological and biochemical mechanisms responsible are unknown. The investigators will test the hypotheses that cardiac work is significantly greater in icefishes compared to red-blooded species, and that as temperature increases, the greater cardiac work of icefishes, coupled with reduced blood oxygen-carrying capacity, results in cardiac failure at a lower temperature compared to red-blooded species. They also hypothesize that neuronal function limits thermal tolerance of red-blooded notothenioids. These hypotheses will be tested using a wide variety of experiments. For example, the investigators will measure heart rate concurrently with critical thermal maximum. They will also characterize metabolic and gene-expression responses to elevated temperature and determine if mitochondrial function contributes to thermal tolerance using a variety of techniques. To determine if neuronal function limits thermal tolerance they will quantify behavioral responses to warming of whole animals and to warming of only the brain area. They will also determine if acclimation to warmer temperatures impacts heart function and they will measure activities of a variety of enzymes from central metabolic pathways.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "Antarctica; Amd/Us; FISH; USA/NSF; FIELD INVESTIGATION; AMD; Antarctic Peninsula; LABORATORY; USAP-DC", "locations": "Antarctica; Antarctic Peninsula", "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Crockett, Elizabeth; O\u0027Brien, Kristin", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION; OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repo": "USAP-DC", "repositories": "Dryad; GenBank; USAP-DC", "science_programs": null, "south": null, "title": "Collaborative Research: The Physiological and Biochemical Underpinnings of Thermal Tolerance in Antarctic Notothenioid Fishes", "uid": "p0010084", "west": null}, {"awards": "0839007 Near, Thomas", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "Genetic Sequence Data", "datasets": [{"dataset_uid": "000151", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Genetic Sequence Data", "url": "http://www.ncbi.nlm.nih.gov/genbank/"}], "date_created": "Fri, 22 Nov 2013 00:00:00 GMT", "description": "This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).\u003cbr/\u003e\u003cbr/\u003eThe teleost fish fauna in the waters surrounding Antarctica are completely dominated by a single clade of closely related species, the Notothenioidei. This clade offers an unprecedented opportunity to investigate the effects of deep time paleogeographic transformations and periods of global climate change on lineage diversification and facilitation of adaptive radiation. With over 100 species, the Antarctic notothenioid radiation has been the subject of intensive investigation of biochemical, physiological, and morphological adaptations associated with freezing avoidance in the subzero Southern Ocean marine habitats. However, broadly sampled time-calibrated phylogenetic hypotheses of notothenioids have not been used to examine patterns of adaptive radiation in this clade. The goals of this project are to develop an intensive phylogenomic scale dataset for 90 of the 124 recognized notothenioid species, and use this genomic resource to generate time-calibrated molecular phylogenetic trees. The results of pilot phylogenetic studies indicate a very exciting correlation of the initial diversification of notothenioids with the fragmentation of East Gondwana approximately 80 million years ago, and the origin of the Antarctic Clade adaptive radiation at a time of global cooling and formation of polar conditions in the Southern Ocean, approximately 35 million years ago. This project will provide research experiences for undergraduates, training for a graduate student, and support a post doctoral researcher. In addition the project will include three high school students from New Haven Public Schools for summer research internships.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Near, Thomas", "platforms": "Not provided", "repo": "NCBI GenBank", "repositories": "NCBI GenBank", "science_programs": null, "south": -90.0, "title": "Genomic Approaches to Resolving Phylogenies of Antarctic Notothenioid Fishes", "uid": "p0000497", "west": -180.0}, {"awards": "0636696 DeVries, Arthur", "bounds_geometry": "POLYGON((-68.0025 -52.7599,-67.07254 -52.7599,-66.14258 -52.7599,-65.21262 -52.7599,-64.28266 -52.7599,-63.3527 -52.7599,-62.42274 -52.7599,-61.49278 -52.7599,-60.56282 -52.7599,-59.63286 -52.7599,-58.7029 -52.7599,-58.7029 -53.98242,-58.7029 -55.20494,-58.7029 -56.42746,-58.7029 -57.64998,-58.7029 -58.8725,-58.7029 -60.09502,-58.7029 -61.31754,-58.7029 -62.54006,-58.7029 -63.76258,-58.7029 -64.9851,-59.63286 -64.9851,-60.56282 -64.9851,-61.49278 -64.9851,-62.42274 -64.9851,-63.3527 -64.9851,-64.28266 -64.9851,-65.21262 -64.9851,-66.14258 -64.9851,-67.07254 -64.9851,-68.0025 -64.9851,-68.0025 -63.76258,-68.0025 -62.54006,-68.0025 -61.31754,-68.0025 -60.09502,-68.0025 -58.8725,-68.0025 -57.64998,-68.0025 -56.42746,-68.0025 -55.20494,-68.0025 -53.98242,-68.0025 -52.7599))", "dataset_titles": "Expedition Data; Expedition data of LMG0809", "datasets": [{"dataset_uid": "001504", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0809"}, {"dataset_uid": "001493", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0810"}, {"dataset_uid": "002728", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0809", "url": "https://www.rvdata.us/search/cruise/LMG0809"}], "date_created": "Tue, 04 May 2010 00:00:00 GMT", "description": "Antarctic notothenioid fish evolved antifreeze (AF) proteins that prevent ice crystals that enter their body fluids from growing, and thereby avoid freezing in their icy habitats. However, even in the extreme cold Antarctic marine environment, regional gradations of severity are found. The biological correlate for environmental severity in fish is the endogenous ice load, which likely determines the tolerable limit of environmental severity for notothenioid habitation. The endogenous ice load develops from environmental ice crystals entering through body surfaces and somehow localizing to the spleen. How prone the surface tissues are to ice entry, how ice reaches the spleen, and what the fate of splenic ice is, requires elucidation. Spleen sequestration of ice raises the hypothesis that macrophages may play a role in the translocation and perhaps elimination of AF-bound ice crystals. Antifreeze glycoproteins (AFGP) act in concert with a second, recently discovered antifreeze called antifreeze potentiating protein (AFPP), necessitating an assessment of the contribution of AFPP to freezing avoidance. Recent research suggests that the exocrine pancreas and the anterior stomach, not the liver, synthesize AFGPs and secrete them into the intestine, from where they may be returned to the blood. A GI-to-blood transport is a highly unconventional path for a major plasma protein and also begs the questions, What is the source of blood AFPP?. Why are two distinct AF proteins needed and what is the chronology of their evolution? What genomic changes in the DNA are associated with the development or loss of the antifreeze trait? Experiments described in this proposal address these interrelated questions of environmental, organismal, and evolutionary physiology, and will further our understanding of novel vertebrate physiologies, the limits of environmental adaptation, and climatically driven changes in the genome. The proposed research will (1) determine the temporal and spatial heterogeneity of environmental temperature and iciness in progressively more severe fish habitats in the greater McMurdo Sound area, and in the milder Arthur Harbor at Palmer Station. The splenic ice load in fishes inhabiting these sites will be determined to correlate to environmental severity and habitability. (2) Assess the surface tissue site of ice entry and their relative barrier properties in intact fish and isolated tissues preparations (3) Assess the role of immune cells in the fate of endogenous ice, (4) determine whether the blood AFGPs are from intestinal/rectal uptake, (5) examine the contribution of AFPP to the total blood AF activity (6) evaluate the progression of genomic changes in the AFGP locus across Notothenioidei as modulated by disparate thermal environments, in four selected species through the analyses of large insert DNA BAC clones. The origin and evolution of AFPP will be examined also by analyzing BAC clones encompassing the AFPP genomic locus. The broader impacts of the proposed research include training of graduate and undergraduate students in research approaches ranging from physical field measurements to cutting edge genomics. Undergraduate research projects have lead to co-authored publications and will continue to do so. Outreach includes establishing Wiki websites on topics of Antarctic fish biology and freeze avoidance, providing advisory services to the San Francisco Science Exploratorium, and making BAC libraries available to interested polar biologists. This research theme has repeatedly received national and international science news coverage and will continue to be disseminated to the public in that manner.", "east": -58.7029, "geometry": "POINT(-63.3527 -58.8725)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ADCP", "is_usap_dc": false, "keywords": "R/V LMG", "locations": null, "north": -52.7599, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Devries, Arthur", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "R2R", "repositories": "R2R", "science_programs": null, "south": -64.9851, "title": "Environmental, Organismal and Evolutionary Physiology of Freeze Avoidance in Antarctic Notothenioid Fishes", "uid": "p0000560", "west": -68.0025}, {"awards": "0437887 Sidell, Bruce", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "Differential Expression of Oxygen-binding Proteins in Antarctic Fishes Affects Nitric Oxide-mediated Pathways of Angiogenesis and Mitochondrial Biogenesis; Expedition Data; Expedition data of LMG0705; Expedition data of LMG0706", "datasets": [{"dataset_uid": "002712", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0705", "url": "https://www.rvdata.us/search/cruise/LMG0705"}, {"dataset_uid": "001534", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0705"}, {"dataset_uid": "002713", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0706", "url": "https://www.rvdata.us/search/cruise/LMG0706"}, {"dataset_uid": "600039", "doi": "10.15784/600039", "keywords": "Biota; Oceans; Pot; Sample/collection Description; Sample/Collection Description; Southern Ocean; Trawl", "people": "Sidell, Bruce", "repository": "USAP-DC", "science_program": null, "title": "Differential Expression of Oxygen-binding Proteins in Antarctic Fishes Affects Nitric Oxide-mediated Pathways of Angiogenesis and Mitochondrial Biogenesis", "url": "https://www.usap-dc.org/view/dataset/600039"}], "date_created": "Sun, 06 Dec 2009 00:00:00 GMT", "description": "The polar ocean presently surrounding Antarctica is the coldest, most thermally stable marine environment on earth. Because oxygen solubility in seawater is inversely proportional to temperature, the cold Antarctic seas are an exceptionally oxygen-rich aquatic habitat. Eight families of a single perciform suborder, the Notothenioidei, dominate the present fish fauna surrounding Antarctica. Notothenioids account for approximately 35% of fish species and 90% of fish biomass south of the Antarctic Polar Front. Radiation of closely related notothenioid species thus has occurred rapidly and under a very unusual set of conditions: relative oceanographic isolation from other faunas due to circumpolar currents and deep ocean trenches surrounding the continent, chronically, severely cold water temperatures, very high oxygen availability, very low levels of niche competition in a Southern Ocean depauperate of species subsequent to a dramatic crash in species diversity of fishes that occurred sometime between the mid-Tertiary and present. These features make Antarctic notothenioid fishes an uniquely attractive group for the study of physiological and biochemical adaptations to cold body temperature. \u003cbr/\u003eFew distinctive features of Antarctic fishes are as unique as the pattern of expression of oxygen-binding proteins in one notothenioid family, the Channichthyidae (Antarctic icefishes). All channichthyid icefishes lack the circulating oxygen-binding protein, hemoglobin (Hb); the intracellular oxygen-binding protein, myoglobin (Mb) is not uniformly expressed in species of this family. Both proteins are normally considered essential for adequate delivery of oxygen to aerobically poised tissues of animals. To compensate for the absence of Hb, icefishes have developed large hearts, rapidly circulate a large blood volume and possess elaborate vasculature of larger lumenal diameter than is seen in red-blooded fishes. Loss of Mb expression in oxidative muscles correlates with dramatic elevation in density of mitochondria within the cell, although each individual organelle is less densely packed with respiratory proteins. \u003cbr/\u003eWithin the framework of oxygen movement, the adaptive significance of greater vascular density and mitochondrial populations is understandable but mechanisms underlying development of these characteristics remain unknown. The answer may lie in another major function of both Hb and Mb, degradation of the ubiquitous bioactive compound, nitric oxide (NO). The research will test the hypothesis that loss of hemoprotein expression in icefishes has resulted in an increase in levels of NO that mediate modification of vascular systems and expansion of mitochondrial populations in oxidative tissues. The objectives of the proposal are to quantify the vascular density of retinas in +Hb and -Hb notothenioid species, to characterize NOS isoforms and catalytic activity in retina and cardiac muscle of Antarctic notothenioid fishes, to evaluate level of expression of downstream factors implicated in angiogenesis (in retinal tissue) and mitochondrial biogenesis (in cardiac muscle), and to determine whether inhibition of NOS in vivo results in regression of angiogenic and mitochondrial biogenic responses in icefishes. Broader impacts range from basic biology, through training of young scientists, to enhanced understanding of clinically relevant biomedical processes.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ADCP; IN SITU/LABORATORY INSTRUMENTS \u003e PRESSURE/HEIGHT METERS \u003e PRESSURE SENSORS; IN SITU/LABORATORY INSTRUMENTS \u003e PHOTON/OPTICAL DETECTORS \u003e TURBIDITY METERS", "is_usap_dc": true, "keywords": "R/V LMG; Not provided", "locations": null, "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Sidell, Bruce", "platforms": "Not provided; WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "R2R", "repositories": "R2R; USAP-DC", "science_programs": null, "south": -90.0, "title": "Collaborative Research: Differential Expression of Oxygen-binding Proteins in Antarctic Fishes Affects Nitric Oxide-mediated Pathways of Angiogenesis and Mitochondrial Biogenesis.", "uid": "p0000527", "west": -180.0}]

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