[{"awards": "2137376 Porazinska, Dorota", "bounds_geometry": null, "date_created": "Wed, 23 Jul 2025 00:00:00 GMT", "description": "This dataset includes an Amplicon Sequence Variant (ASV) table derived from 16S and 18S metabarcoding of microbial communities exposed to nutrient enrichment under microcosm conditions. The original communities came from cryoconite hole sediments from Canada Glacier, Taylor Valley, Antarctica. Homogenized sediments were then distributed among 20 sterile Petri dishes (60 mm), each with 25 g dry weight equivalent (31.25 g wet sediment based on 25% water content) and assigned to five experimental treatments. Treatments consisted of 1) Bulk, sediments that received neither water nor nutrients, 2) Control, sediments that received additions of 1 ml of water, 3) +N, sediments that received 1 ml of ammonium nitrate solution to achieve 4 \u00b5mol N * g soil-1, 3) +P, sediments that received 1 ml of sodium phosphate dibasic solution to achieve 2 \u00b5mol P * g soil-1, and 5) +NP, sediments that received 1 ml of combined ammonium nitrate and sodium phosphate dibasic to achieve 4 \u00b5mol N + 2\u00b5mol P * g soil-1. Each treatment was replicated five times and incubated in a freeze-thaw growth chamber with temperatures cycling between 1\u00b0C and 3\u00b0C and light between ~100 Lux (5 hrs/day) and ~10,000 Lux (19 hrs per day) to imitate natural cryoconite holes conditions during austral summers.", "east": null, "geometry": null, "keywords": "Antarctica; Cryosphere; McMurdo Dry Valleys; Microbial Diversity; Taylor Valley", "locations": "Antarctica; Taylor Valley; McMurdo Dry Valleys", "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "persons": "Porazinska, Dorota", "project_titles": "Collaborative Research: Role of Nutrient Limitation and Viral Interactions on Antarctic Microbial Community Assembly: A Cryoconite Microcosm Study", "projects": [{"proj_uid": "p0010418", "repository": "USAP-DC", "title": "Collaborative Research: Role of Nutrient Limitation and Viral Interactions on Antarctic Microbial Community Assembly: A Cryoconite Microcosm Study"}], "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": null, "title": "Metabarcoding (16S, 18S) ASV Table from Canada Glacier cryoconite sediments incubated with nutrients", "uid": "601957", "west": null}, {"awards": "1141936 Foreman, Christine", "bounds_geometry": ["POINT(112.086 79.468)"], "date_created": "Thu, 01 Jan 2015 00:00:00 GMT", "description": "This award supports a detailed, molecular level characterization of dissolved organic carbon and microbes in Antarctic ice cores. Using the most modern biological (genomic), geochemical techniques, and advanced chemical instrumentation researchers will 1) optimize protocols for collecting, extracting and amplifying DNA from deep ice cores suitable for use in next generation pyrosequencing; 2) determine the microbial diversity within the ice core; and 3) obtain and analyze detailed molecular characterizations of the carbon in the ice by ultrahigh resolution Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS). With this pilot study investigators will be able to quantify the amount of material (microbial biomass and carbon) required to perform these characterizations, which is needed to inform future ice coring projects. The ultimate goal will be to develop protocols that maximize the yield, while minimizing the amount of ice required. The broader impacts include education and outreach at both the local and national levels. As a faculty mentor with the American Indian Research Opportunities and BRIDGES programs at Montana State University, Foreman will serve as a mentor to a Native American student in the lab during the summer months. Susan Kelly is an Education and Outreach Coordinator with a MS degree in Geology and over 10 years of experience in science outreach. She will coordinate efforts for comprehensive educational collaboration with the Hardin School District on the Crow Indian Reservation in South-central Montana.\n", "east": 112.086, "geometry": ["POINT(112.086 79.468)"], "keywords": "Antarctica; Biota; Genetic Sequences; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Ice Core Records; Paleoclimate; WAIS Divide; WAIS Divide Ice Core", "locations": "Antarctica; WAIS Divide", "north": 79.468, "nsf_funding_programs": null, "persons": "Foreman, Christine", "project_titles": "Molecular Level Characterization of Dissolved Organic Carbon and Microbial Diversity in the WAIS Divide Replicate Core", "projects": [{"proj_uid": "p0000342", "repository": "USAP-DC", "title": "Molecular Level Characterization of Dissolved Organic Carbon and Microbial Diversity in the WAIS Divide Replicate Core"}], "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": "WAIS Divide Ice Core", "south": 79.468, "title": "Molecular Level Characterization of Dissolved Organic Carbon and Microbial Diversity in the WAIS Divide Replicate Core", "uid": "600133", "west": 112.086}, {"awards": "0338342 Foreman, Christine; 0338260 Chin, Yu-Ping", "bounds_geometry": ["POINT(166.167 -77.55)"], "date_created": "Thu, 01 Jan 2009 00:00:00 GMT", "description": "Dissolved organic matter (DOM) is a significant chemical component in aquatic systems because it acts as an important carbon source for microorganisms, absorbs harmful radiation in sunlight, is able to complex metals, and can participate in important biogeochemical reactions. This study will investigate the biogeochemical cycling of DOM in a small coastal Antarctic pond, Pony Lake, located on Cape Royds, Ross Island. Because there are no higher plants present at this site all of the DOM in this lake is derived from microorganisms. Thus, Pony Lake is an ideal site to study the effect of physical, chemical, and microbial processes on the composition and character of the DOM pool. Finally, Pony Lake is also an ideal site to collect an International Humic Substances Society (IHSS) fulvic acid standard. Unlike other IHSS standards, this standard will not contain DOM components derived from higher land plants. To better understand the role of physical influences, the project will study the changes in the DOM pool as the lake evolves from ice-covered to ice-free conditions during the summer, as well as the relationship of DOM to the observed turnover of dominant microbial communities in the lake. Scientists will also monitor changes in microbial abundance, diversity, and productivity that may occur during the ice to open-water transition period. This research will provide much needed information regarding the relationship between microbial diversity and DOM biogeochemistry. Middle school science students will be active participants in this project through the Internet, while scientists are in the field, and in the lab.", "east": 166.167, "geometry": ["POINT(166.167 -77.55)"], "keywords": "Antarctica; Chemistry:fluid; Chemistry:Fluid; Critical Zone; Ross Island; Sample/collection Description; Sample/Collection Description; Water Samples", "locations": "Ross Island; Antarctica", "north": -77.55, "nsf_funding_programs": null, "persons": "Chin, Yu-Ping; Foreman, Christine", "project_titles": "Collaborative Research: Biogeochemistry of Dissolved Organic Matter in Pony Lake, Ross Island", "projects": [{"proj_uid": "p0000548", "repository": "USAP-DC", "title": "Collaborative Research: Biogeochemistry of Dissolved Organic Matter in Pony Lake, Ross Island"}], "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -77.55, "title": "Biogeochemistry of Dissolved Organic Matter in Pony Lake, Ross Island", "uid": "600168", "west": 166.167}]

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Dataset Title/Abstract/Map	NSF Award(s)	Date Created	PIs / Scientists	Project Links	Abstract	Bounds Geometry	Geometry	Selected	Visible
Metabarcoding (16S, 18S) ASV Table from Canada Glacier cryoconite sediments incubated with nutrients	2137376	2025-07-23	Porazinska, Dorota	Collaborative Research: Role of Nutrient Limitation and Viral Interactions on Antarctic Microbial Community Assembly: A Cryoconite Microcosm Study	This dataset includes an Amplicon Sequence Variant (ASV) table derived from 16S and 18S metabarcoding of microbial communities exposed to nutrient enrichment under microcosm conditions. The original communities came from cryoconite hole sediments from Canada Glacier, Taylor Valley, Antarctica. Homogenized sediments were then distributed among 20 sterile Petri dishes (60 mm), each with 25 g dry weight equivalent (31.25 g wet sediment based on 25% water content) and assigned to five experimental treatments. Treatments consisted of 1) Bulk, sediments that received neither water nor nutrients, 2) Control, sediments that received additions of 1 ml of water, 3) +N, sediments that received 1 ml of ammonium nitrate solution to achieve 4 µmol N * g soil-1, 3) +P, sediments that received 1 ml of sodium phosphate dibasic solution to achieve 2 µmol P * g soil-1, and 5) +NP, sediments that received 1 ml of combined ammonium nitrate and sodium phosphate dibasic to achieve 4 µmol N + 2µmol P * g soil-1. Each treatment was replicated five times and incubated in a freeze-thaw growth chamber with temperatures cycling between 1°C and 3°C and light between ~100 Lux (5 hrs/day) and ~10,000 Lux (19 hrs per day) to imitate natural cryoconite holes conditions during austral summers.	[]	[]	false	false
Molecular Level Characterization of Dissolved Organic Carbon and Microbial Diversity in the WAIS Divide Replicate Core	1141936	2015-01-01	Foreman, Christine	Molecular Level Characterization of Dissolved Organic Carbon and Microbial Diversity in the WAIS Divide Replicate Core	This award supports a detailed, molecular level characterization of dissolved organic carbon and microbes in Antarctic ice cores. Using the most modern biological (genomic), geochemical techniques, and advanced chemical instrumentation researchers will 1) optimize protocols for collecting, extracting and amplifying DNA from deep ice cores suitable for use in next generation pyrosequencing; 2) determine the microbial diversity within the ice core; and 3) obtain and analyze detailed molecular characterizations of the carbon in the ice by ultrahigh resolution Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS). With this pilot study investigators will be able to quantify the amount of material (microbial biomass and carbon) required to perform these characterizations, which is needed to inform future ice coring projects. The ultimate goal will be to develop protocols that maximize the yield, while minimizing the amount of ice required. The broader impacts include education and outreach at both the local and national levels. As a faculty mentor with the American Indian Research Opportunities and BRIDGES programs at Montana State University, Foreman will serve as a mentor to a Native American student in the lab during the summer months. Susan Kelly is an Education and Outreach Coordinator with a MS degree in Geology and over 10 years of experience in science outreach. She will coordinate efforts for comprehensive educational collaboration with the Hardin School District on the Crow Indian Reservation in South-central Montana.	["POINT(112.086 79.468)"]	["POINT(112.086 79.468)"]	false	false
Biogeochemistry of Dissolved Organic Matter in Pony Lake, Ross Island	0338342 0338260	2009-01-01	Chin, Yu-Ping; Foreman, Christine	Collaborative Research: Biogeochemistry of Dissolved Organic Matter in Pony Lake, Ross Island	Dissolved organic matter (DOM) is a significant chemical component in aquatic systems because it acts as an important carbon source for microorganisms, absorbs harmful radiation in sunlight, is able to complex metals, and can participate in important biogeochemical reactions. This study will investigate the biogeochemical cycling of DOM in a small coastal Antarctic pond, Pony Lake, located on Cape Royds, Ross Island. Because there are no higher plants present at this site all of the DOM in this lake is derived from microorganisms. Thus, Pony Lake is an ideal site to study the effect of physical, chemical, and microbial processes on the composition and character of the DOM pool. Finally, Pony Lake is also an ideal site to collect an International Humic Substances Society (IHSS) fulvic acid standard. Unlike other IHSS standards, this standard will not contain DOM components derived from higher land plants. To better understand the role of physical influences, the project will study the changes in the DOM pool as the lake evolves from ice-covered to ice-free conditions during the summer, as well as the relationship of DOM to the observed turnover of dominant microbial communities in the lake. Scientists will also monitor changes in microbial abundance, diversity, and productivity that may occur during the ice to open-water transition period. This research will provide much needed information regarding the relationship between microbial diversity and DOM biogeochemistry. Middle school science students will be active participants in this project through the Internet, while scientists are in the field, and in the lab.	["POINT(166.167 -77.55)"]	["POINT(166.167 -77.55)"]	false	false

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