USAP-DC

{"dp_type": "Project", "free_text": "icefishes"}

[{"awards": "1947040 Postlethwait, John; 2232891 Postlethwait, John; 1543383 Postlethwait, John", "bounds_geometry": "POLYGON((-180 -37,-144 -37,-108 -37,-72 -37,-36 -37,0 -37,36 -37,72 -37,108 -37,144 -37,180 -37,180 -42.3,180 -47.6,180 -52.9,180 -58.2,180 -63.5,180 -68.8,180 -74.1,180 -79.4,180 -84.69999999999999,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -84.7,-180 -79.4,-180 -74.1,-180 -68.8,-180 -63.5,-180 -58.2,-180 -52.9,-180 -47.6,-180 -42.300000000000004,-180 -37))", "dataset_titles": "aBSREL tests for episodic diversifying selection on hemoglobin genes in notothenioids.; MEME tests of sites evolving under episodic diversifying selection in notothenioid hemoglobin genes.; Notothenioid hemoglobin protein 3D modeling.; Notothenioid species tree used in the study.; Phylogenetic trees of hemoglobin proteins in notothenioids.; Rates of hemoglobin evolution among genes and across notothenioid species.; RELAX tests for pervasive changes in strength of natural selection on hemoglobin genes in notothenioids.", "datasets": [{"dataset_uid": "601728", "doi": "10.15784/601728", "keywords": "Antarctica; Cold Adaptation; Cryonotothenioid; Dragonfish; Eleginopsioidea; Fish; Gene; Genetic Analysis; Hemoglobin; Icefish; Notothenioid; Notothenioid Fishes; Plunderfish; Sub-Antarctic", "people": "Postlethwait, John; Desvignes, Thomas", "repository": "USAP-DC", "science_program": null, "title": "aBSREL tests for episodic diversifying selection on hemoglobin genes in notothenioids.", "url": "https://www.usap-dc.org/view/dataset/601728"}, {"dataset_uid": "601732", "doi": "10.15784/601732", "keywords": "Antarctica; Cold Adaptation; Cryonotothenioid; Dragonfish; Eleginopsioidea; Fish; Genetic Analysis; Hemoglobin; Icefish; Notothenioid; Notothenioid Fishes; Plunderfish; Sub-Antarctic", "people": "Postlethwait, John; Desvignes, Thomas", "repository": "USAP-DC", "science_program": null, "title": "Notothenioid hemoglobin protein 3D modeling.", "url": "https://www.usap-dc.org/view/dataset/601732"}, {"dataset_uid": "601731", "doi": "10.15784/601731", "keywords": "Antarctica; Cold Adaptation; Cryonotothenioid; Dragonfish; Eleginopsioidea; Fish; Genetic Analysis; Hemoglobin; Icefish; Notothenioid; Notothenioid Fishes; Plunderfish; Sub-Antarctic", "people": "Desvignes, Thomas; Postlethwait, John", "repository": "USAP-DC", "science_program": null, "title": "RELAX tests for pervasive changes in strength of natural selection on hemoglobin genes in notothenioids.", "url": "https://www.usap-dc.org/view/dataset/601731"}, {"dataset_uid": "601730", "doi": "10.15784/601730", "keywords": "Antarctica; Cold Adaptation; Cryonotothenioid; Dragonfish; Eleginopsioidea; Fish; Genetic Analysis; Hemoglobin; Icefish; Notothenioid; Notothenioid Fishes; Plunderfish; Sub-Antarctic", "people": "Postlethwait, John; Desvignes, Thomas", "repository": "USAP-DC", "science_program": null, "title": "MEME tests of sites evolving under episodic diversifying selection in notothenioid hemoglobin genes.", "url": "https://www.usap-dc.org/view/dataset/601730"}, {"dataset_uid": "601729", "doi": "10.15784/601729", "keywords": "Antarctica; Cold Adaptation; Cryonotothenioid; Dragonfish; Eleginopsioidea; Fish; Genetic Analysis; Hemoglobin; Icefish; Notothenioid; Notothenioid Fishes; Plunderfish; Sub-Antarctic", "people": "Desvignes, Thomas; Postlethwait, John", "repository": "USAP-DC", "science_program": null, "title": "Rates of hemoglobin evolution among genes and across notothenioid species.", "url": "https://www.usap-dc.org/view/dataset/601729"}, {"dataset_uid": "601722", "doi": "10.15784/601722", "keywords": "Antarctica; Cold Adaptation; Cryonotothenioid; Dragonfish; Eleginopsioidea; Fish; Gene; Hemoglobin; Icefish; Notothenioid; Plunderfish; Sub-Antarctic", "people": "Postlethwait, John; Desvignes, Thomas", "repository": "USAP-DC", "science_program": null, "title": "Phylogenetic trees of hemoglobin proteins in notothenioids.", "url": "https://www.usap-dc.org/view/dataset/601722"}, {"dataset_uid": "601721", "doi": "10.15784/601721", "keywords": "Antarctica; Cold Adaptation; Cryonotothenioid; Dragonfish; Eleginopsioidea; Fish; Gene; Hemoglobin; Icefish; Notothenioid; Plunderfish; Sub-Antarctic", "people": "Postlethwait, John; Desvignes, Thomas", "repository": "USAP-DC", "science_program": null, "title": "Notothenioid species tree used in the study.", "url": "https://www.usap-dc.org/view/dataset/601721"}], "date_created": "Wed, 03 May 2023 00:00:00 GMT", "description": "Antarctic notothenioid fishes, also known as cryonotothenioids, inhabit the icy and highly oxygenated waters surrounding the Antarctic continent after diverging from notothenioids inhabiting more temperate waters. Notothenioid hemoglobin and blood parameters are known to have evolved along with the establishment of stable polar conditions, and among Antarctic notothenioids, icefishes are evolutionary oddities living without hemoglobin following the deletion of all functional hemoglobin genes from their genomes. In this project, we investigate the evolution of hemoglobin genes and gene clusters across the notothenioid radiation until their loss in the icefish ancestor after its divergence from the dragonfish lineage to understand the forces, mechanisms, and potential causes for hemoglobin gene loss in the icefish ancestor.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "FISH; Icefish; Cryonotothenioid; Gene; Plunderfish; Eleginopsioidea; AQUATIC ECOSYSTEMS; Dragonfish; Sub-Antarctic; Notothenioid; Blood; Hemoglobin", "locations": "Sub-Antarctic", "north": -37.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Desvignes, Thomas; Postlethwait, John", "platforms": null, "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -90.0, "title": "Evolution of hemoglobin genes in notothenioid fishes", "uid": "p0010417", "west": -180.0}, {"awards": "1954241 O\u0027\u0027Brien, Kristin", "bounds_geometry": null, "dataset_titles": null, "datasets": null, "date_created": "Tue, 17 Aug 2021 00:00:00 GMT", "description": "Part 1: Non-technical description: Global climate warming is increasing the frequency and severity of low oxygen events in marine and freshwater environments worldwide, and these events threaten the health of aquatic ecosystems and the viability of fish populations. The Southern Ocean surrounding Antarctica has historically been a stable, icy-cold, and oxygen-rich environment, but is now warming at an unprecedented rate and faster than all other regions in the Southern hemisphere. Antarctic fishes have evolved in sub-zero temperatures that have been stable over long periods of time with traits allowing them to thrive in frigid waters, but with diminished resilience to warming temperatures. Presently little is known about the ability of Antarctic fishes to withstand hypoxic, or low-oxygen, conditions that often accompany warming. This research will investigate the hypoxia tolerance of four species of Antarctic fishes, including two species of icefishes that lack the oxygen-carrying protein, hemoglobin, which may compromise their ability to oxygenate tissues under hypoxic conditions. The hypoxia tolerance of four Antarctic fish species will be compared to that of a related fish species inhabiting warmer coastal regions of South America. Physiological and biochemical responses to hypoxia will be evaluated and compared amongst the five species to bolster our predictions of the capacity of Antarctic fishes to cope with a changing environment. This research will provide training opportunities for undergraduate and graduate students, and a postdoctoral research fellow. A year-long seminar series hosted by the Aquarium of the Pacific will feature female scientists who work in Antarctica to inspire youth in the greater Los Angeles area to pursue careers in science. Part 2: Technical description: The overarching hypothesis to be tested in this project is that the long evolution of Antarctic notothenioid fishes in a cold, oxygen-rich environment has reduced their capacity to mount a robust physiological, biochemical, and molecular response to hypoxia compared to related, cold-temperate fish species. Hypoxia tolerance will be compared among the red-blooded Antarctic notothenioids, Notothenia coriiceps and Notothenia rossii; the hemoglobinless Antarctic icefishes, Chaenocephalus aceratus and Chionodraco rastrospinosus; and the basal, cold-temperate notothenioid, Eleginops maclovinus, a species that has never inhabited waters south of the Polar Front. The minimum level of oxygen required to sustain maintenance metabolic requirements (O2crit) will be quantified. Animals will then be exposed to 65% of O2crit for 48 hours, and responses to hypoxia will be evaluated by measuring hematocrit and hemoglobin levels, as well as metabolites in brain, liver, glycolytic and cardiac muscles. Maximal activities of key enzymes of aerobic and anaerobic metabolism will be quantified to assess capacities for synthesizing ATP in hypoxic conditions. Gill remodeling will be analyzed using light and scanning electron microscopy. The molecular response to hypoxia will be characterized in liver and brains by quantifying levels of the master transcriptional regulator of oxygen homeostasis, hypoxia-inducible factor-1 (HIF-1), and hypoxic gene expression will be quantified using RNA-Seq. Cell cultures will be used to determine if a previously identified insertion mutation in notothenioid HIF-1 affects the ability of HIF-1 to drive gene expression and thus, hypoxia tolerance. The results of this project will provide the most comprehensive assessment of the hypoxia tolerance of Antarctic fishes to date. Broader impacts include research training opportunities for undergraduate and graduate students and a postdoctoral research associate, with a focus on involving Native Alaskan students in research. In partnership with the Aquarium of the Pacific, a year-long public seminar series will be held, showcasing the research and careers of 9 women who conduct research in Antarctica. The goal of the series is to cultivate and empower a community of middle and high school students in the greater Los Angeles area to pursue their interests in science and related fields, and to enhance the public engagement capacities of research scientists so that they may better inspire youth and early career scientists in STEM fields. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "Palmer Station; FIELD SURVEYS; USAP-DC; AMD; USA/NSF; Amd/Us; FISH", "locations": "Palmer Station", "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "O\u0027Brien, Kristin", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS", "repositories": null, "science_programs": null, "south": null, "title": "ANT LIA: Hypoxia Tolerance in Notothenioid Fishes", "uid": "p0010246", "west": null}, {"awards": "1341602 Crockett, Elizabeth; 1341663 O\u0027Brien, Kristin", "bounds_geometry": null, "dataset_titles": "Acclimation of cardiovascular function in Notothenia coriiceps; Adrenergic and adenosinergic regulation of the cardiovascular system in the Antarctic icefish Chaenocephalus aceratus; Cardiac metabolism in Antarctic fishes in response to an acute increase in temperature; Chaenocephalus aceratus HIF-1A mRNA, complete cds; Chionodraco rastrospinosus HIF-1A mRNA, partial cds; Effects of acute warming on cardiovascular performance of Antarctic fishes; Eleginops maclovinus HIF-1A mRNA, partial cds; Gymnodraco acuticeps HIF-1A mRNA, partial cds; Hypoxia response of hearts of Antarctic fishes; Maximum cardiac performance of Antarctic fishes that lack haemoglobin and myoglobin: exploring the effect of warming on nature\u2019s natural knockouts; Measurements of splenic contraction in Antarctic fishes; Mitochondrial membranes in cardiac muscle from Antarctic notothenioid fishes vary in phospholipid composition and membrane fluidity; Notothenia coriiceps HIF-1A mRNA, complete cds; Parachaenichthys charcoti HIF-1A mRNA, partial cds; Physical, chemical, and functional properties of neuronal membranes vary between species of Antarctic notothenioids differing in thermal tolerance; Thermal sensitivity of membrane fluidity and integrity in hearts of Antarctic fishes that vary in expression of hemoglobin and myoglobin", "datasets": [{"dataset_uid": "601410", "doi": "10.15784/601410", "keywords": "Antarctica; Antarctic Peninsula; Biota; Fish", "people": "O\u0027Brien, Kristin; Crockett, Elizabeth; Egginton, Stuart; Axelsson, Michael; Farrell, Anthony; Joyce, William", "repository": "USAP-DC", "science_program": null, "title": "Effects of acute warming on cardiovascular performance of Antarctic fishes", "url": "https://www.usap-dc.org/view/dataset/601410"}, {"dataset_uid": "200189", "doi": null, "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "Eleginops maclovinus HIF-1A mRNA, partial cds", "url": "https://www.ncbi.nlm.nih.gov/nuccore/KX950830"}, {"dataset_uid": "601407", "doi": "10.15784/601407", "keywords": "Antarctica; Antarctic Peninsula", "people": "Axelsson, Michael; Joyce, William; O\u0027Brien, Kristin", "repository": "USAP-DC", "science_program": null, "title": "Measurements of splenic contraction in Antarctic fishes", "url": "https://www.usap-dc.org/view/dataset/601407"}, {"dataset_uid": "200184", "doi": "10.5061/dryad.83vc5", "keywords": null, "people": null, "repository": "Dryad", "science_program": null, "title": "Maximum cardiac performance of Antarctic fishes that lack haemoglobin and myoglobin: exploring the effect of warming on nature\u2019s natural knockouts", "url": "https://doi.org/10.5061/dryad.83vc5"}, {"dataset_uid": "200185", "doi": "10.5061/dryad.k90h35k", "keywords": null, "people": null, "repository": "Dryad", "science_program": null, "title": "Mitochondrial membranes in cardiac muscle from Antarctic notothenioid fishes vary in phospholipid composition and membrane fluidity", "url": "https://doi.org/10.5061/dryad.k90h35k"}, {"dataset_uid": "200186", "doi": "10.5061/dryad.qm0b25h", "keywords": null, "people": null, "repository": "Dryad", "science_program": null, "title": "Physical, chemical, and functional properties of neuronal membranes vary between species of Antarctic notothenioids differing in thermal tolerance", "url": "https://doi.org/10.5061/dryad.qm0b25h"}, {"dataset_uid": "200187", "doi": null, "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "Chaenocephalus aceratus HIF-1A mRNA, complete cds", "url": "https://www.ncbi.nlm.nih.gov/nuccore/KX950828"}, {"dataset_uid": "601408", "doi": "10.15784/601408", "keywords": "Antarctica; Antarctic Peninsula", "people": "Farrell, Anthony; Crockett, Elizabeth; Axelsson, Michael; O\u0027Brien, Kristin; Egginton, Stuart; Joyce, William", "repository": "USAP-DC", "science_program": null, "title": "Acclimation of cardiovascular function in Notothenia coriiceps", "url": "https://www.usap-dc.org/view/dataset/601408"}, {"dataset_uid": "200188", "doi": null, "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "Notothenia coriiceps HIF-1A mRNA, complete cds", "url": "https://www.ncbi.nlm.nih.gov/nuccore/KX950829"}, {"dataset_uid": "601409", "doi": "10.15784/601409", "keywords": "Antarctica; Antarctic Peninsula", "people": "Joyce, Michael; O\u0027Brien, Kristin; Egginton, Stuart; Farrell, Anthony; Axelsson, Michael", "repository": "USAP-DC", "science_program": null, "title": "Adrenergic and adenosinergic regulation of the cardiovascular system in the Antarctic icefish Chaenocephalus aceratus", "url": "https://www.usap-dc.org/view/dataset/601409"}, {"dataset_uid": "601414", "doi": "10.15784/601414", "keywords": "Antarctica; Antarctic Peninsula", "people": "Evans, Elizabeth; Farnoud, Amir; Crockett, Elizabeth; O\u0027Brien, Kristin", "repository": "USAP-DC", "science_program": null, "title": "Thermal sensitivity of membrane fluidity and integrity in hearts of Antarctic fishes that vary in expression of hemoglobin and myoglobin", "url": "https://www.usap-dc.org/view/dataset/601414"}, {"dataset_uid": "200190", "doi": null, "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "Parachaenichthys charcoti HIF-1A mRNA, partial cds", "url": "https://www.ncbi.nlm.nih.gov/nuccore/KX950833"}, {"dataset_uid": "601405", "doi": "10.15784/601405", "keywords": "Antarctica; Antarctic Peninsula", "people": "O\u0027Brien, Kristin", "repository": "USAP-DC", "science_program": null, "title": "Cardiac metabolism in Antarctic fishes in response to an acute increase in temperature", "url": "https://www.usap-dc.org/view/dataset/601405"}, {"dataset_uid": "200191", "doi": null, "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "Gymnodraco acuticeps HIF-1A mRNA, partial cds", "url": "https://www.ncbi.nlm.nih.gov/nuccore/kx950832"}, {"dataset_uid": "200192", "doi": null, "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "Chionodraco rastrospinosus HIF-1A mRNA, partial cds", "url": "https://www.ncbi.nlm.nih.gov/nuccore/kx950831"}, {"dataset_uid": "601406", "doi": "10.15784/601406", "keywords": "Antarctica; Antarctic Peninsula", "people": "O\u0027Brien, Kristin", "repository": "USAP-DC", "science_program": null, "title": "Hypoxia response of hearts of Antarctic fishes", "url": "https://www.usap-dc.org/view/dataset/601406"}], "date_created": "Wed, 26 Feb 2020 00:00:00 GMT", "description": "The ocean surrounding Antarctica is home to an extraordinary assemblage of fishes, dominated by a single group that are extremely well-suited to life in icy waters and which are of significant ecological importance there. Of great concern is the capacity of these fishes to withstand increases in temperature as the region of the Western Antarctic Peninsula warms at a rate faster than any other area in the Southern hemisphere. One particular group of Antarctic fishes, known as the icefishes, are particularly vulnerable to increases in temperature because unlike all other vertebrates on earth, icefishes are white-blooded due to their lack of the oxygen-binding protein hemoglobin. This greatly reduces their capacity to transport and deliver oxygen to tissues compared to red-blooded Antarctic fishes. Previous studies have shown that icefishes are indeed less tolerant to elevations in temperature but the underlying factors are completely unknown. Additionally, it is not understood if red- or white-blooded Antarctic fishes can adjust, or acclimate, to modest increases in temperature, similar to those changes in temperature the animals might experience as the earth warms. The investigators will determine if heart function and/or nervous system function limits thermal tolerance of Antarctic fishes, and will determine their capacity to acclimate to warmer temperatures. The project will further the NSF goal of training new generations of scientists by training graduate and undergraduate students. In addition, the project will collaborate with a high school biology teacher from a school which serves a largely minority student body. The students will learn about the marine environment, and will construct a camera to be used in the field to learn more about Antarctic fishes. Two students and the teacher will also attend a summer marine biology internship program. Antarctic fishes within the suborder Notothenioidei (called \"notothenioids\") are among the organisms on earth least able to deal with changes in temperature. The hemoglobinless icefish are even less able to withstand temperature changes than are red-blooded notothenioids. While this is well documented, the underlying physiological and biochemical mechanisms responsible are unknown. The investigators will test the hypotheses that cardiac work is significantly greater in icefishes compared to red-blooded species, and that as temperature increases, the greater cardiac work of icefishes, coupled with reduced blood oxygen-carrying capacity, results in cardiac failure at a lower temperature compared to red-blooded species. They also hypothesize that neuronal function limits thermal tolerance of red-blooded notothenioids. These hypotheses will be tested using a wide variety of experiments. For example, the investigators will measure heart rate concurrently with critical thermal maximum. They will also characterize metabolic and gene-expression responses to elevated temperature and determine if mitochondrial function contributes to thermal tolerance using a variety of techniques. To determine if neuronal function limits thermal tolerance they will quantify behavioral responses to warming of whole animals and to warming of only the brain area. They will also determine if acclimation to warmer temperatures impacts heart function and they will measure activities of a variety of enzymes from central metabolic pathways.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "Antarctica; Amd/Us; FISH; USA/NSF; FIELD INVESTIGATION; AMD; Antarctic Peninsula; LABORATORY; USAP-DC", "locations": "Antarctica; Antarctic Peninsula", "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Crockett, Elizabeth; O\u0027Brien, Kristin", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION; OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repo": "USAP-DC", "repositories": "Dryad; GenBank; USAP-DC", "science_programs": null, "south": null, "title": "Collaborative Research: The Physiological and Biochemical Underpinnings of Thermal Tolerance in Antarctic Notothenioid Fishes", "uid": "p0010084", "west": null}, {"awards": "1341701 Bilyk, Kevin", "bounds_geometry": null, "dataset_titles": "Antarctic Ice fish; submission ID #SRP113562", "datasets": [{"dataset_uid": "000206", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Antarctic Ice fish; submission ID #SRP113562", "url": "https://www.ncbi.nlm.nih.gov/genbank/"}], "date_created": "Mon, 31 Jul 2017 00:00:00 GMT", "description": "This work will broaden our knowledge and insights into genetic trait loss or change accompanying species evolution in general as well as within the uniquely isolated and frigid Southern Ocean. The system of oxygen-carrying and related proteins being studied is very important to human health and the two proteins being specifically studied in this work (haptoglobin and hemopexin) have crucial roles in preventing excess iron loading in the kidneys. As such, the project has the potential to contribute novel insights that could be valuable to medical science. The project will also further the NSF goals of training new generations of scientists and of making scientific discoveries available to the general public. The lead principal investigator on the project is an early career scientist whose career development will be enhanced by this project. It will also support the training of several undergraduate students in molecular biology, protein biochemistry, and appreciation of the unique Antarctic fish fauna and environment. The project will contribute to a content-rich web site that will bring to the public the history of biological discoveries and sciences on fishes of the Southern Ocean and through this project the investigators will contribute to an annual polar event at a children\u0027s science museum. The Antarctic icefishes have thrived despite the striking evolutionary loss of the normally indispensable respiratory protein hemoglobin in all species and myoglobin in some. Studies over the past decades have predominately focused on the mechanisms behind hemoprotein losses and the resulting compensatory adaptations in these fish, while evolutionary impact of such losses on the supporting protein genes and functions has remained unaddressed. This project investigates the evolutionary fate of two important partner proteins, the hemoglobin scavenger haptoglobin and the heme scavenger hemopexin (heme groups are the iron-containing functional group of proteins such as hemoglobin and myoglobin). With the permanent hemoglobin-null state in Antarctic icefishes, and particularly in dual hemoglobin- and myoglobin-null species, the preservation of a functional haptoglobin would seem unessential and the role of hemopexin likely diminished. This project seeks to resolve whether co-evolutionary loss or reduction of these supporting proteins occurred with the extinction of the hemoglobin trait in the icefishes, and the molecular mechanisms underlying such changes. The investigators envisage the cold and oxygen rich marine environment as the start of a cascade of relaxation of selection pressures. Initially this would have obviated the need for maintaining functional oxygen carrying proteins, ultimately leading to their permanent loss. These events in turn would have relaxed the maintenance of the network of supporting systems, leading to additional trait loss or change.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Bilyk, Kevin", "platforms": "Not provided", "repo": "NCBI GenBank", "repositories": "NCBI GenBank", "science_programs": null, "south": null, "title": "Evolutionary Fates of Hemoglobin and Heme Scavengers in White-blooded Antarctic Icefishes", "uid": "p0000396", "west": null}, {"awards": "1043576 Crockett, Elizabeth; 1043781 O\u0027Brien, Kristin", "bounds_geometry": "POLYGON((-64.45 -63.467,-64.2633 -63.467,-64.0766 -63.467,-63.8899 -63.467,-63.7032 -63.467,-63.5165 -63.467,-63.3298 -63.467,-63.1431 -63.467,-62.9564 -63.467,-62.7697 -63.467,-62.583 -63.467,-62.583 -63.5653,-62.583 -63.6636,-62.583 -63.7619,-62.583 -63.8602,-62.583 -63.9585,-62.583 -64.0568,-62.583 -64.1551,-62.583 -64.2534,-62.583 -64.3517,-62.583 -64.45,-62.7697 -64.45,-62.9564 -64.45,-63.1431 -64.45,-63.3298 -64.45,-63.5165 -64.45,-63.7032 -64.45,-63.8899 -64.45,-64.0766 -64.45,-64.2633 -64.45,-64.45 -64.45,-64.45 -64.3517,-64.45 -64.2534,-64.45 -64.1551,-64.45 -64.0568,-64.45 -63.9585,-64.45 -63.8602,-64.45 -63.7619,-64.45 -63.6636,-64.45 -63.5653,-64.45 -63.467))", "dataset_titles": "Electronic fishing logs; Expedition data of LMG1104; Redox Balance in Antarctic Notothenioid Fishes", "datasets": [{"dataset_uid": "002687", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG1104", "url": "https://www.rvdata.us/search/cruise/LMG1104"}, {"dataset_uid": "600390", "doi": "10.15784/600390", "keywords": "Antarctica; Biota; Southern Ocean", "people": "Crockett, Elizabeth", "repository": "USAP-DC", "science_program": null, "title": "Electronic fishing logs", "url": "https://www.usap-dc.org/view/dataset/600390"}, {"dataset_uid": "600382", "doi": "10.15784/600382", "keywords": "Antarctica; Antarctic Peninsula; Biota; Fish; Sample/collection Description; Sample/Collection Description; Southern Ocean", "people": "O\u0027Brien, Kristin", "repository": "USAP-DC", "science_program": null, "title": "Redox Balance in Antarctic Notothenioid Fishes", "url": "https://www.usap-dc.org/view/dataset/600382"}], "date_created": "Tue, 06 Dec 2016 00:00:00 GMT", "description": "Antarctic channichthyid icefishes are stunning examples of the unique physiological traits that can arise during evolution in a constantly cold environment. Icefishes are the only vertebrates that as adults, lack the circulating oxygen-binding protein hemoglobin (Hb); several species within this family also lack the intracellular oxygen-binding protein myoglobin (Mb) in their heart ventricle. The loss of Hb and Mb has resulted in striking modifications in the cardiovascular system to ensure adequate tissue oxygenation, some of which are energetically costly. Recent indicate there may be at least one benefit to not expressing these heme-centered proteins - oxidized proteins and lipids are higher in red-blooded notothenioids compared to icefishes. The research will address the hypothesis that the loss of Hb and Mb reduces oxidative stress in icefishes compared to red-blooded notothenioid fishes, resulting in a lower rate of protein turnover and energetic cost savings. Specifically, the project will (1) Characterize levels of oxidative stress in red- and white-blooded notothenioid fishes, (2) Determine if red- and white-blooded notothenioids differ in their regulation of iron, (3) Determine if lower levels of oxidized proteins in icefishes result in lower rates of protein turnover and energetic cost savings, and (4) Determine if oxygen-binding proteins promote oxidative stress in-vivo and in-vitro. The results will contribute to the understanding of iron-catalyzed oxidative stress, which is associated with the progression of Alzheimer\u0027s, Parkinson\u0027s and cardiovascular diseases. Moreover, the research will increase understanding of factors related to iron metabolism and oxidative stress in notothenioid fishes that may have played key roles in the success of channichthyid icefishes. The broader impacts include development of a website will enable teachers and students to learn more about the fascinating biology of Antarctic icefishes, as well as the impacts of global climate change and commercial fishing activities on Antarctic fishes. Additionally, Alaska Native high school and undergraduate students will be involved in research at the University of Alaska, Fairbanks.", "east": -62.583, "geometry": "POINT(-63.5165 -63.9585)", "instruments": "EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS", "is_usap_dc": true, "keywords": "Not provided; R/V LMG", "locations": null, "north": -63.467, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Crockett, Elizabeth; O\u0027Brien, Kristin", "platforms": "Not provided; WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "R2R", "repositories": "R2R; USAP-DC", "science_programs": null, "south": -64.45, "title": "Collaborative research: Redox Balance in Antarctic Notothenioid fishes: Do Icefishes have an Advantage?", "uid": "p0000320", "west": -64.45}, {"awards": "0741301 O\u0027Brien, Kristin; 1142720 Crockett, Elizabeth", "bounds_geometry": "POLYGON((-64.45 -63.29,-64.249 -63.29,-64.048 -63.29,-63.847 -63.29,-63.646 -63.29,-63.445 -63.29,-63.244 -63.29,-63.043 -63.29,-62.842 -63.29,-62.641 -63.29,-62.44 -63.29,-62.44 -63.370999999999995,-62.44 -63.452,-62.44 -63.533,-62.44 -63.614,-62.44 -63.69499999999999,-62.44 -63.775999999999996,-62.44 -63.857,-62.44 -63.937999999999995,-62.44 -64.01899999999999,-62.44 -64.1,-62.641 -64.1,-62.842 -64.1,-63.043 -64.1,-63.244 -64.1,-63.445 -64.1,-63.646 -64.1,-63.847 -64.1,-64.048 -64.1,-64.249 -64.1,-64.45 -64.1,-64.45 -64.01899999999999,-64.45 -63.937999999999995,-64.45 -63.857,-64.45 -63.775999999999996,-64.45 -63.69499999999999,-64.45 -63.614,-64.45 -63.533,-64.45 -63.452,-64.45 -63.370999999999995,-64.45 -63.29))", "dataset_titles": "Linkages among Mitochondrial Form, Function and Thermal Tolerance of Antarctic Notothenioid Fishes", "datasets": [{"dataset_uid": "600084", "doi": "10.15784/600084", "keywords": "Biota; Oceans; Pot; Southern Ocean; Trawl", "people": "O\u0027Brien, Kristin", "repository": "USAP-DC", "science_program": null, "title": "Linkages among Mitochondrial Form, Function and Thermal Tolerance of Antarctic Notothenioid Fishes", "url": "https://www.usap-dc.org/view/dataset/600084"}], "date_created": "Sat, 30 Nov 2013 00:00:00 GMT", "description": "Abstract Antarctic notothenioid fishes have evolved in the Southern Ocean for 10-14 MY under an unusual set of circumstances. Their characteristics include the complete absence of the circulating oxygen-binding protein, hemoglobin (Hb) within the Channichthyid (Icefish) family of notothenioids. Moreover, some species within the 16 members of this family have also lost the ability to express the oxygen-binding and storage protein, myoglobin (Mb) in cardiac muscle. Our previous work has determined that the loss of Hb and/or Mb is correlated with significant increases in densities of mitochondria within oxidative tissues, and extensive remodeling of these vital organelles. To date, nothing is known about how modifications in mitochondrial architecture of icefishes affect organelle function, or more importantly, how they affect organismal-level physiology. Most critical for Antarctic fishes is that mitochondrial characteristics have been linked to how well ectotherms can withstand increases in temperature. This collaborative research project will address the hypothesis that the unusual mitochondrial architecture of Antarctic Channichthyids has led to changes in function that impact their ability to withstand elevations in temperature. Specifically, the research will (1) determine if the unusual mitochondrial architecture of icefishes affects function and contributes to organismal thermal sensitivity, (2) identify differences in organismal thermal tolerance between red- and white- blooded notothenioids, (3) identify molecular mechanisms regulating changes in mitochondrial structure in icefishes. The results may establish channichthyid icefishes as a sentinel taxon for signaling the impact of global warming on the Southern Ocean. Broad impacts of this project will be realized by participation of high school biology teachers in field work through cooperation with the ARMADA project at the University of Rhode Island, as well as graduate education.", "east": -62.44, "geometry": "POINT(-63.445 -63.695)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -63.29, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Crockett, Elizabeth; O\u0027Brien, Kristin", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -64.1, "title": "Collaborative Research: Linkages among Mitochondrial Form, Function and Thermal Tolerance of Antarctic Notothenioid Fishes", "uid": "p0000483", "west": -64.45}, {"awards": "0125890 Sidell, Bruce", "bounds_geometry": "POLYGON((-68.1413 -52.6755,-67.47503 -52.6755,-66.80876 -52.6755,-66.14249 -52.6755,-65.47622 -52.6755,-64.80995 -52.6755,-64.14368 -52.6755,-63.47741 -52.6755,-62.81114 -52.6755,-62.14487 -52.6755,-61.4786 -52.6755,-61.4786 -53.8957,-61.4786 -55.1159,-61.4786 -56.3361,-61.4786 -57.5563,-61.4786 -58.7765,-61.4786 -59.9967,-61.4786 -61.2169,-61.4786 -62.4371,-61.4786 -63.6573,-61.4786 -64.8775,-62.14487 -64.8775,-62.81114 -64.8775,-63.47741 -64.8775,-64.14368 -64.8775,-64.80995 -64.8775,-65.47622 -64.8775,-66.14249 -64.8775,-66.80876 -64.8775,-67.47503 -64.8775,-68.1413 -64.8775,-68.1413 -63.6573,-68.1413 -62.4371,-68.1413 -61.2169,-68.1413 -59.9967,-68.1413 -58.7765,-68.1413 -57.5563,-68.1413 -56.3361,-68.1413 -55.1159,-68.1413 -53.8957,-68.1413 -52.6755))", "dataset_titles": "Expedition Data; Expedition data of LMG0304; Expedition data of LMG0304A", "datasets": [{"dataset_uid": "002706", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0304", "url": "https://www.rvdata.us/search/cruise/LMG0304"}, {"dataset_uid": "001704", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0304"}, {"dataset_uid": "001597", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0505"}, {"dataset_uid": "001596", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0506"}, {"dataset_uid": "002707", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0304A", "url": "https://www.rvdata.us/search/cruise/LMG0304A"}, {"dataset_uid": "002708", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0304A", "url": "https://www.rvdata.us/search/cruise/LMG0304A"}], "date_created": "Tue, 04 May 2010 00:00:00 GMT", "description": "Notothenioid fishes that dominate the fish fauna surrounding Antarctica have been evolving for 10-14 million years at a nearly constant body temperature of ~0C throughout their life histories. As a result, this group of animals is uniquely suited to studies aimed at understanding and identifying features of physiology and biochemistry that result from the process of evolution at cold body temperature. This project has three major objectives aimed at examining adaptations for life in cold environments: \u003cbr/\u003e\u003cbr/\u003e1. Identify the amino acid substitutions in the fatty acid-binding pocket of fatty acyl CoA synthetase (FACS) that explain its substrate specificity. Fatty acids are a major fuel of energy metabolism in Antarctic fishes. FACS catalyzes the condensation of CoASH and fatty acids to fatty acyl CoA esters, a step required for subsequent metabolism of these important compounds. This research may permit us to resolve the specific amino acid substitutions that explain both substrate specificity and preservation of catalytic rate of notothenioid FACS at cold physiological temperatures.\u003cbr/\u003e\u003cbr/\u003e2. Produce a rigorous biochemical and biophysical characterization of the intracellular calcium-binding protein, parvalbumin, from white axial musculature of Antarctic fishes. Parvalbumin plays a pivotal role in facilitating the relaxation phase of fast-contracting muscles and is a likely site of strong selective pressure. Preliminary data strongly indicate that the protein from Antarctic fishes has been modified to ensure function at cold temperature. A suite of physical techniques will be used to determine dissociation constants of Antarctic fish parvalbumins for calcium and magnesium and unidirectional rate constants of ion-dissociation from the protein. Full-length cDNA clones for Antarctic fish parvalbumin(s) will permit deduction of primary amino acid sequence These data will yield insight into structural elements that permit the protein from notothenioid fishes to function at very cold body temperature.\u003cbr/\u003e\u003cbr/\u003e3. Conduct a broad survey of the pattern of cardiac myoglobin expression in the Suborder Notothenoidei. Previous work has indicated a variable pattern of presence or absence of the intracellular oxygen-binding protein, myoglobin (Mb), in hearts of one family of Antarctic notothenioid fishes (Channichthyidae; icefishes). Because Mb is of physiological value in species that express the protein, the observed pattern of interspecific expression has been attributed to unusually low niche competition in the Southern Ocean. This leads to the prediction that similar loss of cardiac Mb should be observed in other notothenioid taxa. This part of the project will survey for the presence and absence of cardiac Mb in as many notothenioid species as possible and, if Mb-lacking species are detected, will extend analyses to determine the mechanism(s) responsible for loss of its expression using molecular biological techniques.", "east": -61.4786, "geometry": "POINT(-64.80995 -58.7765)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PRESSURE/HEIGHT METERS \u003e PRESSURE SENSORS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ADCP", "is_usap_dc": false, "keywords": "R/V LMG", "locations": null, "north": -52.6755, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Sidell, Bruce; Detrich, H. William", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "R2R", "repositories": "R2R", "science_programs": null, "south": -64.8775, "title": "Cold Body Temperature as an Evolutionary Shaping force in the Physiology of Antarctic Fishes", "uid": "p0000241", "west": -68.1413}, {"awards": "0437887 Sidell, Bruce", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "Differential Expression of Oxygen-binding Proteins in Antarctic Fishes Affects Nitric Oxide-mediated Pathways of Angiogenesis and Mitochondrial Biogenesis; Expedition Data; Expedition data of LMG0705; Expedition data of LMG0706", "datasets": [{"dataset_uid": "002712", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0705", "url": "https://www.rvdata.us/search/cruise/LMG0705"}, {"dataset_uid": "002713", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0706", "url": "https://www.rvdata.us/search/cruise/LMG0706"}, {"dataset_uid": "001534", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0705"}, {"dataset_uid": "600039", "doi": "10.15784/600039", "keywords": "Biota; Oceans; Pot; Sample/collection Description; Sample/Collection Description; Southern Ocean; Trawl", "people": "Sidell, Bruce", "repository": "USAP-DC", "science_program": null, "title": "Differential Expression of Oxygen-binding Proteins in Antarctic Fishes Affects Nitric Oxide-mediated Pathways of Angiogenesis and Mitochondrial Biogenesis", "url": "https://www.usap-dc.org/view/dataset/600039"}], "date_created": "Sun, 06 Dec 2009 00:00:00 GMT", "description": "The polar ocean presently surrounding Antarctica is the coldest, most thermally stable marine environment on earth. Because oxygen solubility in seawater is inversely proportional to temperature, the cold Antarctic seas are an exceptionally oxygen-rich aquatic habitat. Eight families of a single perciform suborder, the Notothenioidei, dominate the present fish fauna surrounding Antarctica. Notothenioids account for approximately 35% of fish species and 90% of fish biomass south of the Antarctic Polar Front. Radiation of closely related notothenioid species thus has occurred rapidly and under a very unusual set of conditions: relative oceanographic isolation from other faunas due to circumpolar currents and deep ocean trenches surrounding the continent, chronically, severely cold water temperatures, very high oxygen availability, very low levels of niche competition in a Southern Ocean depauperate of species subsequent to a dramatic crash in species diversity of fishes that occurred sometime between the mid-Tertiary and present. These features make Antarctic notothenioid fishes an uniquely attractive group for the study of physiological and biochemical adaptations to cold body temperature. \u003cbr/\u003eFew distinctive features of Antarctic fishes are as unique as the pattern of expression of oxygen-binding proteins in one notothenioid family, the Channichthyidae (Antarctic icefishes). All channichthyid icefishes lack the circulating oxygen-binding protein, hemoglobin (Hb); the intracellular oxygen-binding protein, myoglobin (Mb) is not uniformly expressed in species of this family. Both proteins are normally considered essential for adequate delivery of oxygen to aerobically poised tissues of animals. To compensate for the absence of Hb, icefishes have developed large hearts, rapidly circulate a large blood volume and possess elaborate vasculature of larger lumenal diameter than is seen in red-blooded fishes. Loss of Mb expression in oxidative muscles correlates with dramatic elevation in density of mitochondria within the cell, although each individual organelle is less densely packed with respiratory proteins. \u003cbr/\u003eWithin the framework of oxygen movement, the adaptive significance of greater vascular density and mitochondrial populations is understandable but mechanisms underlying development of these characteristics remain unknown. The answer may lie in another major function of both Hb and Mb, degradation of the ubiquitous bioactive compound, nitric oxide (NO). The research will test the hypothesis that loss of hemoprotein expression in icefishes has resulted in an increase in levels of NO that mediate modification of vascular systems and expansion of mitochondrial populations in oxidative tissues. The objectives of the proposal are to quantify the vascular density of retinas in +Hb and -Hb notothenioid species, to characterize NOS isoforms and catalytic activity in retina and cardiac muscle of Antarctic notothenioid fishes, to evaluate level of expression of downstream factors implicated in angiogenesis (in retinal tissue) and mitochondrial biogenesis (in cardiac muscle), and to determine whether inhibition of NOS in vivo results in regression of angiogenic and mitochondrial biogenic responses in icefishes. Broader impacts range from basic biology, through training of young scientists, to enhanced understanding of clinically relevant biomedical processes.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ADCP; IN SITU/LABORATORY INSTRUMENTS \u003e PRESSURE/HEIGHT METERS \u003e PRESSURE SENSORS; IN SITU/LABORATORY INSTRUMENTS \u003e PHOTON/OPTICAL DETECTORS \u003e TURBIDITY METERS", "is_usap_dc": true, "keywords": "R/V LMG; Not provided", "locations": null, "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Sidell, Bruce", "platforms": "Not provided; WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "R2R", "repositories": "R2R; USAP-DC", "science_programs": null, "south": -90.0, "title": "Collaborative Research: Differential Expression of Oxygen-binding Proteins in Antarctic Fishes Affects Nitric Oxide-mediated Pathways of Angiogenesis and Mitochondrial Biogenesis.", "uid": "p0000527", "west": -180.0}]

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