USAP-DC

{"dp_type": "Project", "free_text": "Transcriptome"}

[{"awards": "2532581 Robinson, Rebecca", "bounds_geometry": "POLYGON((-175 -54,-172.5 -54,-170 -54,-167.5 -54,-165 -54,-162.5 -54,-160 -54,-157.5 -54,-155 -54,-152.5 -54,-150 -54,-150 -55.3,-150 -56.6,-150 -57.9,-150 -59.2,-150 -60.5,-150 -61.8,-150 -63.1,-150 -64.4,-150 -65.7,-150 -67,-152.5 -67,-155 -67,-157.5 -67,-160 -67,-162.5 -67,-165 -67,-167.5 -67,-170 -67,-172.5 -67,-175 -67,-175 -65.7,-175 -64.4,-175 -63.1,-175 -61.8,-175 -60.5,-175 -59.2,-175 -57.9,-175 -56.6,-175 -55.3,-175 -54))", "dataset_titles": null, "datasets": null, "date_created": "Wed, 17 Jun 2026 00:00:00 GMT", "description": "Diatoms are microscopic algae that live in oceans and other aquatic environments. Different diatom species thrive in different conditions, and so diatom fossils can be used as historical records of ocean environments, including the availability of nutrients such as nitrogen and silicon that are important components of ocean ecosystems. This RAPID proposal aims to understand how environmental conditions and the species of diatom may impact these records. The investigators will use recently collected samples from the Southern Ocean to determine how different species react to varying amounts of nitrogen and silicon. These culture-derived data will then be used to ground-truth field-collected data. This is expected to improve our use of diatom fossil information to understand past ocean conditions. Diatom fossils provide information on past surface ocean nutrient conditions through the analysis of cell-bound nitrogen and silicon isotopic composition of frustules. Recent data suggested that simple fractionation models do not always explain spatial trends or seasonal variation. To improve understanding of isotopic fractionation variability, this project aims to evaluate gene expression, diatom silicification, and diatom-bound nitrogen isotope values under varying nutrient conditions using recently collected, difficult to maintain Antarctic marine diatom isolates. The isolates were collected during the 2024-2025 spring diatom bloom in the Southern Ocean. In addition to accomplishing combined measurements of isotopic relationships and transcriptional response to nutrient conditions for understudied environmentally relevant diatom species, this work will aid in ground truthing in situ cruise data and improve reconstruction of past surface ocean conditions. This award reflects NSF\u0027\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027\u0027s intellectual merit and broader impacts review criteria.", "east": -150.0, "geometry": "POINT(-162.5 -60.5)", "instruments": null, "is_usap_dc": true, "keywords": "NITROGEN; Transcriptome; Laboratory Investigation; ECOLOGICAL DYNAMICS; Southern Ocean", "locations": "Southern Ocean", "north": -54.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Robinson, Rebecca", "platforms": null, "repositories": null, "science_programs": null, "south": -67.0, "title": "RAPID: Linking gene expression, silicification, and diatom-bound nitrogen isotopes in field isolated diatom species from the Southern Ocean", "uid": "p0010569", "west": -175.0}, {"awards": "2148730 Mikucki, Jill", "bounds_geometry": null, "dataset_titles": "LC-MS/MS Metabolomics Community Detection Methods; Massilia sp. DJPM01 Genome sequencing and assembly; Raw mass spectra of soluble metabolites from \"Multi-technique characterization of iron reduction by an Antarctic Shewanella: an analog system for putative Martian biosignature identification\"; Transcriptome of Shewanella sp. BF02_Schw", "datasets": [{"dataset_uid": "200543", "doi": "10.5281/zenodo.18148893", "keywords": null, "people": null, "repository": "Zenodo", "science_program": null, "title": "LC-MS/MS Metabolomics Community Detection Methods", "url": "https://zenodo.org/records/18148894"}, {"dataset_uid": "200538", "doi": "", "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "Massilia sp. DJPM01 Genome sequencing and assembly", "url": "https://www.ncbi.nlm.nih.gov/bioproject/929211"}, {"dataset_uid": "200539", "doi": "", "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "Transcriptome of Shewanella sp. BF02_Schw", "url": "https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA1261965"}, {"dataset_uid": "200540", "doi": "10.5281/zenodo.15389915", "keywords": null, "people": null, "repository": "Zenodo", "science_program": null, "title": "Raw mass spectra of soluble metabolites from \"Multi-technique characterization of iron reduction by an Antarctic Shewanella: an analog system for putative Martian biosignature identification\"", "url": "https://zenodo.org/records/15389915"}], "date_created": "Wed, 24 Dec 2025 00:00:00 GMT", "description": "Antarctica habitats are unlike any others found on the planet with extreme combined variations in cold, salinity, moisture content and light availability. Soils and sediments from the Antarctic McMurdo Dry Valley\u2019s region harbor unique microorganisms that can cope with multiple extreme conditions, however, how extremophile microbes specifically function or respond to change in these extreme habitats is still largely unknown. This team is utilizing previously collected Antarctic McMurdo Dry Valleys (MDV) samples to evaluate differences in microbial extremophile function in response to changes in strong salinity gradients caused by warming temperatures. Comparative analyses of gene expression patterns and secondary metabolite production are being undertaken in lab-based microcosm studies with varying salinities. This work is helping to elucidate the genetic underpinnings for life in Antarctica and show how Antarctic biota evolve and adapt to a changing environment. Specifically, the work is showing how environmentally relevant changes in salinity will induce differential expression of secondary metabolites in isolates previously collected from the hypersaline Don Juan Pond in the western end of Wright Valley, Victoria Land in the Dry Valleys region. Transcriptomes of cell cultures under varying salinities are being sequenced and compared to determine significant changes to cellular metabolism, particularly secondary metabolic pathways. Additional previously collected sample types (soils, glacier ice, sediments, and brines) collected from a range of salinities are being used to refine methodologies for future field work and provide training opportunities for students. The program is designed to provide strong opportunities to integrate STEM research with undergraduate education, especially at Smith College, a predominantly undergraduate women\u2019s institution. At least one graduate student and 4 undergraduates will be involved in the project, and some will participate in presenting results at international meetings. The program is also developing related curriculum in partnership with education specialists to be used with middle school students. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "Secondary Metabolites; Microbial Activity; COMMUNITY DYNAMICS; ", "locations": "", "north": null, "nsf_funding_programs": "Antarctic Earth Sciences; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Mikucki, Jill; Giddings, Lesley-Ann", "platforms": null, "repo": "Zenodo", "repositories": "GenBank; Zenodo", "science_programs": null, "south": null, "title": "Collaborative Research: RUI: Molecular Sentinels: Secondary Metabolites as Indicators of Environmental Change in an Antarctic Polar Desert", "uid": "p0010553", "west": null}, {"awards": "2142913 Tresguerres, Martin; 2142914 Baker, Bill; 2142912 Murray, Alison", "bounds_geometry": "POLYGON((-180 -60,-168 -60,-156 -60,-144 -60,-132 -60,-120 -60,-108 -60,-96 -60,-84 -60,-72 -60,-60 -60,-60 -62,-60 -64,-60 -66,-60 -68,-60 -70,-60 -72,-60 -74,-60 -76,-60 -78,-60 -80,-72 -80,-84 -80,-96 -80,-108 -80,-120 -80,-132 -80,-144 -80,-156 -80,-168 -80,180 -80,178 -80,176 -80,174 -80,172 -80,170 -80,168 -80,166 -80,164 -80,162 -80,160 -80,160 -78,160 -76,160 -74,160 -72,160 -70,160 -68,160 -66,160 -64,160 -62,160 -60,162 -60,164 -60,166 -60,168 -60,170 -60,172 -60,174 -60,176 -60,178 -60,-180 -60))", "dataset_titles": null, "datasets": null, "date_created": "Thu, 17 Oct 2024 00:00:00 GMT", "description": "Non-technical description Marine invertebrates often have mutually beneficial partnerships with microorganisms that biosynthesize compounds with nutritive or defensive functions and are integral for survival. Additionally, these \u201cnatural products\u201d often have bioactive properties with human health applications fighting infection or different types of cancer. This project focuses on the ascidian (\u201csea squirt\u201d) Synoicum adareanum, found in the Anvers Island region of the Antarctic Peninsula, and was recently discovered to contain high levels of a natural product, palmerolide A (palA) in its tissues. The microorganism that produces palA is a new bacterial species, Candidatus Synoicihabitans palmerolidicus, found in a persistent partnership with the sea squirt. There is still much to be learned about the fundamental properties of this sea squirt-microbe-palA system including the geographical range of the animal-microbe partnership, its chemical and microbiome complexity and diversity, and the biological effect of palA in the sea squirt. To address these questions, this multidisciplinary research team will investigate the sea squirt-microbiome partnership in the Antarctic Peninsula and McMurdo Sound regions of the Ross Sea using a state-of-the-art strategy that will advance our understanding of the structural and functional features of the sea squirt and microbiome in detail, and reveal the roles that the palA natural product plays in the host ecology in its native Antarctic seafloor habitat. The project will broaden diversity and provide new opportunities for early career students and postdoctoral researchers to participate in field and laboratory-based research that builds an integrative understanding of Antarctic marine biology, ecology, physiology and chemistry. In addition, advancing the understanding of palA and its biological properties may be of future benefit to biomedicine and human health. Technical description Marine invertebrates and their associated microbiomes can produce bioactive natural products; in fact, \u003e600 such compounds have been identified in species from polar waters. Although such compounds are typically hypothesized to serve ecological roles in host survival through deterring predation, fouling, and microbial infection, in most cases neither the producing organism nor the genome-encoded biosynthetic enzymes are known. This project will study an emerging biosynthetic system from a polar ascidian-microbe association that produces palA, a natural product with bioactivity against the proton-pumping enzyme V-type H+-ATPase (VHA). The objectives include: (i) Determining the microbiome composition, metabolome complexity, palA levels, and mitochondrial DNA sequence of S. adareanum morphotypes at sites in the Antarctic Peninsula and in McMurdo Sound, (ii) Characterizing the Synoicum microbiome using a multi-omics strategy, and (iii) Assessing the potential for co-occurrence of Ca. S. palmerolidicus-palA-VHA in host tissues, and (iv) exploring the role of palA in modulating VHA activity in vivo and its effects on ascidian-microbe ecophysiology. Through a coupled study of palA-producing and non-producing S. adareanum specimens, structural and functional features of the ascidian microbiome metagenome will be characterized to better understand the relationship between predicted secondary metabolite pathways and whether they are expressed in situ using a paired metatranscriptome sequencing and secondary metabolite detection strategy. Combined with tissue co-localization results, functional ecophysiological assays aim to determine the roles that the natural product plays in the host ecology in its native Antarctic seafloor habitat. The contributions of the project will inform this intimate host-microbial association in which the ascidian host bioaccumulates VHA-inhibiting palA, yet its geo-spatial distribution, cellular localization, ecological and physiological role(s) are not known. In addition to elucidating the ecophysiological roles of palA in their native ascidian-microbe association, the results will contribute to the success of translational science, which aligns with NSF\u2019s interests in promoting basic research that leads to advances in Biotechnology and Bioeconomy. The project will also broaden diversity and provide new opportunities for early career students and postdoctoral researchers to participate in field and laboratory-based research that builds an integrative understanding of Antarctic marine biology, ecology, physiology and chemistry. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 160.0, "geometry": "POINT(-130 -70)", "instruments": null, "is_usap_dc": true, "keywords": "ECOSYSTEM FUNCTIONS; BACTERIA/ARCHAEA; BENTHIC; R/V NBP; Antarctic Peninsula; ANIMALS/INVERTEBRATES", "locations": "Antarctic Peninsula", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Baker, Bill; Murray, Alison; Tresguerres, Martin", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repositories": null, "science_programs": null, "south": -80.0, "title": "Collaborative Research: ANT LIA: Diving into the Ecology of an Antarctic Ascidian-Microbiome-Palmerolide Association using a Multi-omic and Functional Approach", "uid": "p0010485", "west": -60.0}, {"awards": "2138994 Kocot, Kevin; 2138993 Gerken, Sarah", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "Invertebrate Zoology Collection", "datasets": [{"dataset_uid": "200519", "doi": "", "keywords": null, "people": null, "repository": "Alabama Museum of Natural History", "science_program": null, "title": "Invertebrate Zoology Collection", "url": "https://arctos.database.museum/almnh_inv"}], "date_created": "Tue, 20 Sep 2022 00:00:00 GMT", "description": "Part I: General description Cumaceans are small crustaceans, commonly known as comma shrimp, that live in muddy or sandy bottom environments in marine waters. Cumaceans are important for the diet of fish, birds, and even grey whales. This research program is assessing cumacean diversity and adaptation in different regions of Antarctica and evaluate this organisms adaptations using molecular methods to a changing Antarctic region. The research stands to significantly advance understanding of invertebrate adaptations to cold, stable habitats and responses to changes in those habitats. In addition, this project is advancing understanding of the biology of Cumacea, a globally diverse and biologically important group of animals. Targeted training of early career students and professionals in cumacean biology, molecular techniques, and bioinformatics is included as part of the program. A workshop at the Los Angeles County Natural History Museum will also train 10 additional graduate students, with a focus on training for underrepresented groups. Project outreach also includes social media, outreach to schools in very diverse school districts in Anchorage, AK, and creation of museum events and an exhibit at the Alabama Museum of Natural History. Finally, engagement by the team in activities related to the National Ocean Science Bowl promotes broad engagement with high school students for Antarctic science learning. Part II: Technical Description The overarching goal of this research is to use cumaceans as a model system to explore invertebrate adaptations to the changing Antarctic. This project is leveraging integrative taxonomy, functional, comparative and evolutionary genomics, and phylogenetic comparative methods to understand the true diversity of Cumacea in the Antarctic. The team is identifying genes and gene families experiencing expansions, selection, or significant differential expression, generating a broadly sampled and robust phylogenetic framework for the Antarctic Cumacea based on transcriptomes and genomes, and exploring rates and timing of diversification. The project is providing important information related to gene gain/loss, positive selection, and differential gene expression as a function of adaptation of organisms to Antarctic habitats. Phylogenomic analyses is providing a robust phylogenetic framework for understudied Southern Ocean Cumacea. At the start of this project, only one Antarctic transcriptome was published for this organism. This project is generating sequenced genomes from 8 species, about 250 transcriptomes from about 70 species, and approximately 470 COI and 16S amplicon barcodes from about 100 species. Curated morphological reference collections will be deposited at the Smithsonian, Los Angeles County Natural History Museum and in the New Zealand National Water and Atmospheric Research collection at Greta Point to assist future researchers in identification of Antarctic cumaceans. Beyond the immediate scope of the current project, the genomic resources will be able to be leveraged by members of the polar biology and invertebrate zoology communities for diverse other uses ranging from PCR primer development to inference of ancestral population sizes. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "Benthic; SHIPS; Antarctic Peninsula; Antarctica; Biodiversity; Peracarida; ARTHROPODS; East Antarctica; Chile; BENTHIC; Cumacea; Ross Sea; Crustacea", "locations": "Antarctica; East Antarctica; Chile; Ross Sea; Antarctic Peninsula", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Polar Special Initiatives; Antarctic Organisms and Ecosystems", "paleo_time": "NOT APPLICABLE", "persons": "Gerken, Sarah; Kocot, Kevin", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e SHIPS", "repo": "Alabama Museum of Natural History", "repositories": "Alabama Museum of Natural History", "science_programs": null, "south": -90.0, "title": "Collaborative Research: ANT LIA: Cumacean -Omics to Measure Mode of Adaptation to Antarctica (COMMAA)", "uid": "p0010379", "west": -180.0}, {"awards": "2053726 Hofmann, Gretchen", "bounds_geometry": "POLYGON((163 -77,163.4 -77,163.8 -77,164.2 -77,164.6 -77,165 -77,165.4 -77,165.8 -77,166.2 -77,166.6 -77,167 -77,167 -77.1,167 -77.2,167 -77.3,167 -77.4,167 -77.5,167 -77.6,167 -77.7,167 -77.8,167 -77.9,167 -78,166.6 -78,166.2 -78,165.8 -78,165.4 -78,165 -78,164.6 -78,164.2 -78,163.8 -78,163.4 -78,163 -78,163 -77.9,163 -77.8,163 -77.7,163 -77.6,163 -77.5,163 -77.4,163 -77.3,163 -77.2,163 -77.1,163 -77))", "dataset_titles": "Analyses combining ATAC-seq, RRBS, and RNA-seq data for purple urchins", "datasets": [{"dataset_uid": "200288", "doi": "", "keywords": null, "people": null, "repository": "GitHub", "science_program": null, "title": "Analyses combining ATAC-seq, RRBS, and RNA-seq data for purple urchins", "url": "https://github.com/snbogan/Sp_RRBS_ATAC"}], "date_created": "Thu, 14 Apr 2022 00:00:00 GMT", "description": "Part 1: Non-technical description: With support from the Office of Polar Programs, this project will evaluate how an important part of the food web in the coastal ocean of Antarctica will respond to climate change. The focal study organism in the plankton is a shelled mollusk, the Antarctic pteropod, Limacina helicina antarctica, an Southern Ocean organism that this known to respond to climate driven changes in ocean acidification and ocean warming. Ocean acidification, the lowering of ocean pH via the absorption of atmospheric carbon dioxide in the surface of the ocean, is a change in the ocean that is expected to cross deleterious thresholds of pH within decades. This study will improve understanding of how pteropods will respond, which will provide insight into predicting the resilience of the Antarctic marine ecosystem during future changes, one of the planet\u2019s last marine wildernesses. The project will use tools of molecular biology to examine specifically how gene expression is modulated in the pteropods, and further, how the changes and regulation of genes act to resist the stress of low pH and high temperature. In addition, this project supports the training of Ph.D. graduate students and advances the goal of inclusive excellence in STEM and in marine sciences, in particular. The students involved in this project are from groups traditionally under-represented in marine science including first-generation college students. Overall, the project contributes to the development of the U.S. work force and contributes to diversity and inclusive excellence in the geosciences. Part 2: Technical description: The overarching goal of this project is to investigate the molecular response of the Antarctic thecosome pteropod, Limacina helicina antarctica to ocean acidification (OA) and ocean warming. The project will investigate changes in the epigenome of juvenile L. h. antarctica, by assessing the dynamics of DNA methylation in response to three scenarios of environmental conditions that were simulated in laboratory mesocosm CO2 experiments: (1) present-day pCO2 conditions for summer and winter, (2) future ocean acidification expected within 10-15 years, and (3) a multiple stressor experiment to investigate synergistic interaction of OA and high temperature stress. Recent lab-based mesocosm experiment research showed significant changes in the dynamics of global DNA methylation in the pteropod genome, along with variation in gene expression in response to abiotic changes. Thus, it is clear that juvenile L. h. antarctica are capable of mounting a substantial epigenetic response to ocean acidification. However, it is not known how DNA methylation, as an epigenetic process, is modulating changes in the transcriptome. In order to address this gap in the epigenetic knowledge regarding pteropods, the project will use next-generation sequencing approaches (e.g., RNA sequencing and reduced representation bisulfite sequencing) to integrate changes in methylation status with changes in gene expression in juvenile pteropods. Overall, this investigation is an important step in exploring environmental transcriptomics and phenotypic plasticity of an ecologically important member of Southern Ocean macrozoooplankton in response to anthropogenic climate change. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 167.0, "geometry": "POINT(165 -77.5)", "instruments": null, "is_usap_dc": true, "keywords": "USAP-DC; McMurdo Sound; Amd/Us; FIELD INVESTIGATION; USA/NSF; AMD; MARINE ECOSYSTEMS; ANIMALS/INVERTEBRATES", "locations": "McMurdo Sound", "north": -77.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Hofmann, Gretchen", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION", "repo": "GitHub", "repositories": "GitHub", "science_programs": null, "south": -78.0, "title": "The Role of the Epigenetic Mechanism, DNA Methylation, in the Tolerance and Resistance of Antarctic Pteropods to Ocean Acidification and Warming", "uid": "p0010313", "west": 163.0}, {"awards": "1746087 Tarrant, Ann", "bounds_geometry": "POLYGON((-80 -60,-77.5 -60,-75 -60,-72.5 -60,-70 -60,-67.5 -60,-65 -60,-62.5 -60,-60 -60,-57.5 -60,-55 -60,-55 -61,-55 -62,-55 -63,-55 -64,-55 -65,-55 -66,-55 -67,-55 -68,-55 -69,-55 -70,-57.5 -70,-60 -70,-62.5 -70,-65 -70,-67.5 -70,-70 -70,-72.5 -70,-75 -70,-77.5 -70,-80 -70,-80 -69,-80 -68,-80 -67,-80 -66,-80 -65,-80 -64,-80 -63,-80 -62,-80 -61,-80 -60))", "dataset_titles": "Calanoides acutus: Transcriptome and gene expression data; BioProject PRJNA757455; Calanus propinquus: Transcriptome and gene expression data; BioProject PRJNA669816; Expedition data of LMG1901; Rhincalanus gigas: Transcriptome and gene expression data; BioProject PRJNA666170", "datasets": [{"dataset_uid": "200283", "doi": "", "keywords": null, "people": null, "repository": "NCBI", "science_program": null, "title": "Calanus propinquus: Transcriptome and gene expression data; BioProject PRJNA669816", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA669816"}, {"dataset_uid": "200284", "doi": "", "keywords": null, "people": null, "repository": "NCBI", "science_program": null, "title": "Calanoides acutus: Transcriptome and gene expression data; BioProject PRJNA757455", "url": "https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA757455"}, {"dataset_uid": "200125", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG1901", "url": "https://www.rvdata.us/search/cruise/LMG1901"}, {"dataset_uid": "200239", "doi": "", "keywords": null, "people": null, "repository": "NCBI", "science_program": null, "title": "Rhincalanus gigas: Transcriptome and gene expression data; BioProject PRJNA666170", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA666170"}], "date_created": "Fri, 06 Aug 2021 00:00:00 GMT", "description": "Animals in the polar oceans have adapted to dramatic seasonal changes in day length, food availability, and ice cover, as well as to consistently cold waters. This project focuses on the adaptations of copepods - small animals that live in the water column and are an important food source to many different predators. The field studies will take place in the western Antarctic Peninsula, an environment and ecosystem that is rapidly changing. Antarctic copepods have developed particular feeding and behavioral strategies to survive in their very seasonal environment, however it is not known how each of these species will respond to environmental change. The overall goal of this project is to examine and compare these adaptations across species and to understand how each species responds to short-term changes in food availability. The project contains three main objectives: the first objective is to compare the sets of genes across species, especially looking at genes related to storage of energy from food. The second objective is to measure and compare the responses of copepods to changes in food availability. The third objective is to determine how variation across the western Antarctic Pensinsula habitat affects the feeding condition of the copepods. To make the data more useful to the broader research community, a database will be developed enabling easy comparison of genetic information between copepod species. This project will provide hands-on training opportunities to graduate and undergraduate student and will seek to recruit students from underrepresented groups. Results and scientific concepts will be shared through outreach activities, including an expedition blog, a series of interactive animations, and public presentations. Polar marine organisms have adapted to dramatic seasonal changes in photoperiod, light intensity, and ice cover, as well as to cold but stable thermal environments. The western Antarctic Peninsula, the focal region for the field studies, has experienced rapid warming and ice melt. While it is difficult to predict exactly how physical conditions in this region will change, effects on species distributions have already been documented. Large Antarctic copepods in the families Calanidae and Rhincalanidae are dominant components of the mesozooplankton that use different metabolic and behavioral strategies to optimize their use of a highly seasonal food supply. The overall goal of this project is to leverage molecular approaches to examine the physiological and metabolic adaptations at the individual and species level. The project focuses on three main objectives: the first objective is to characterize the gene complement and stage-specific gene expression patterns in Antarctic copepods within an evolutionary context. The second objective is to measure and compare the physiological and molecular responses of juvenile copepods to variable feeding conditions. The third objective is to characterize metabolic variation within natural copepod populations. The metabolically diverse Antarctic copepods also provide an excellent opportunity to compare mechanisms regulating energy storage and utilization and to test hypotheses regarding the roles of specific genes. The field studies will aim to utilize information from an ongoing long term research program (the Palmer Long-Term Ecological Research), which complements the ongoing program and provides extensive context for this project. To make the data more useful to the research community, a database will be developed facilitating comparison of transcriptomes between copepod species. This project will provide hands-on training opportunities to graduate and undergraduate students. Efforts will be made to recruit students who are members of underrepresented minorities. Results and scientific concepts will be broadly disseminated through an expedition blog, a series of interactive animations, and public presentations. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": -55.0, "geometry": "POINT(-67.5 -65)", "instruments": null, "is_usap_dc": true, "keywords": "ARTHROPODS; AMD; PELAGIC; USA/NSF; USAP-DC; PLANKTON; West Antarctic Shelf; Amd/Us; SHIPS", "locations": "West Antarctic Shelf", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Tarrant, Ann", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e SHIPS", "repo": "NCBI", "repositories": "NCBI; R2R", "science_programs": null, "south": -70.0, "title": "Physiological Ecology of \"Herbivorous\" Antarctic Copepods", "uid": "p0010239", "west": -80.0}, {"awards": "1643825 Bucklin, Ann", "bounds_geometry": "POLYGON((-74.57 -60.9,-72.487 -60.9,-70.404 -60.9,-68.321 -60.9,-66.238 -60.9,-64.155 -60.9,-62.072 -60.9,-59.989 -60.9,-57.906 -60.9,-55.823 -60.9,-53.74 -60.9,-53.74 -61.537,-53.74 -62.174,-53.74 -62.811,-53.74 -63.448,-53.74 -64.085,-53.74 -64.722,-53.74 -65.359,-53.74 -65.996,-53.74 -66.633,-53.74 -67.27,-55.823 -67.27,-57.906 -67.27,-59.989 -67.27,-62.072 -67.27,-64.155 -67.27,-66.238 -67.27,-68.321 -67.27,-70.404 -67.27,-72.487 -67.27,-74.57 -67.27,-74.57 -66.633,-74.57 -65.996,-74.57 -65.359,-74.57 -64.722,-74.57 -64.085,-74.57 -63.448,-74.57 -62.811,-74.57 -62.174,-74.57 -61.537,-74.57 -60.9))", "dataset_titles": "Alongtrack data collected continuously by the ship\u0027s underway acquisition system from ARSV Laurence M. Gould cruise LMG1110 in the Southern Ocean in 2011 ; Bucklin, A., R.J. O\u0027Neill, D. Payne (2018) Antarctic salp genome and RNAseq transcriptome from ARSV Laurence M. Gould, Umitaka-Maru, R/V Polarstern LMG1110, UM-08-09, ANT-XXVII-2 in the Southern Ocean. Biological and Chemical Oceanography Data Management Office (BCO-DMO). ; Bucklin, A., R.J. O\u0027Neill, D. Payne (2018) Salp specimen log for genomic and transcriptomic study collected from ARSV Laurence M. Gould, Umitaka-Maru, R/V Polarstern LMG1110, UM-08-09, ANT-XXVII-2. Biological and Chemical Oceanography Data Management Office (BCO-DMO).; CTD data from ARSV Laurence M. Gould LMG1110 in the Southern Ocean from November to December 2011 (Salp_Antarctic project) ; CTD data from MOCNESS tows taken in the Antarctic in 2011 from ARSV Laurence M. Gould LMG1110 in the Southern Ocean from November to December 2011 (Salp_Antarctic project) ; Scientific sampling event log from ARSV Laurence M. Gould LMG1110 in the Southern Ocean from Nov. 2011 (Salp_Antarctic project) ", "datasets": [{"dataset_uid": "200227", "doi": "", "keywords": null, "people": null, "repository": "BCO-DMO", "science_program": null, "title": "Bucklin, A., R.J. O\u0027Neill, D. Payne (2018) Salp specimen log for genomic and transcriptomic study collected from ARSV Laurence M. Gould, Umitaka-Maru, R/V Polarstern LMG1110, UM-08-09, ANT-XXVII-2. Biological and Chemical Oceanography Data Management Office (BCO-DMO).", "url": "https://www.bco-dmo.org/dataset/672600"}, {"dataset_uid": "200229", "doi": "", "keywords": null, "people": null, "repository": "BCO-DMO", "science_program": null, "title": "CTD data from ARSV Laurence M. Gould LMG1110 in the Southern Ocean from November to December 2011 (Salp_Antarctic project) ", "url": "https://www.bco-dmo.org/dataset/559174/data"}, {"dataset_uid": "200231", "doi": "", "keywords": null, "people": null, "repository": "BCO-DMO", "science_program": null, "title": "Alongtrack data collected continuously by the ship\u0027s underway acquisition system from ARSV Laurence M. Gould cruise LMG1110 in the Southern Ocean in 2011 ", "url": "https://www.bco-dmo.org/dataset/3636/data"}, {"dataset_uid": "200232", "doi": "", "keywords": null, "people": null, "repository": "BCO-DMO", "science_program": null, "title": "CTD data from MOCNESS tows taken in the Antarctic in 2011 from ARSV Laurence M. Gould LMG1110 in the Southern Ocean from November to December 2011 (Salp_Antarctic project) ", "url": "https://www.bco-dmo.org/dataset/488871/data"}, {"dataset_uid": "200230", "doi": "", "keywords": null, "people": null, "repository": "BCO-DMO", "science_program": null, "title": "Scientific sampling event log from ARSV Laurence M. Gould LMG1110 in the Southern Ocean from Nov. 2011 (Salp_Antarctic project) ", "url": "https://www.bco-dmo.org/dataset/3565/data"}, {"dataset_uid": "200228", "doi": "", "keywords": null, "people": null, "repository": "BCO-DMO", "science_program": null, "title": "Bucklin, A., R.J. O\u0027Neill, D. Payne (2018) Antarctic salp genome and RNAseq transcriptome from ARSV Laurence M. Gould, Umitaka-Maru, R/V Polarstern LMG1110, UM-08-09, ANT-XXVII-2 in the Southern Ocean. Biological and Chemical Oceanography Data Management Office (BCO-DMO). ", "url": "https://www.bco-dmo.org/dataset/675040/data"}], "date_created": "Sat, 03 Jul 2021 00:00:00 GMT", "description": "The Antarctic salp, Salpa thompsoni, is a gelatinous zooplankton that is an important member in the Southern Ocean pelagic ecosystem. Field studies have documented rapid population growth under favorable environmental conditions, resulting in dense blooms of salps that substantially change the pelagic ecosystem in regards to both structure and processes. Because this zooplankton can proliferate rapidly and it is not readily consumed by upper trophic levels, its periodic dominance has the potential to drastically chance ecosystem energetics as well as change material export to the deep ocean. Completion of a comprehensive reference genome for the Antarctic salp will enable the identification of genes and gene networks underlying physiological responses and allow detection of potential processes driving natural selection and the species? adaptation strategies to the Antarctic Environment. Comparative genomic analysis will add the dimension of time to inferences about organismal adaptation and allow consideration of their potential to adapt to future environmental changes, and will allow examination of novel aspects of genomic evolution found only in the invertebrate class Tunicata. The completed salp reference genome will provide a valuable foundational resource for other scientists working on this species as well as the genomic basis for function and adaptation in the Antarctic. The primary goal of this effort is to examine the rapid genome evolution characteristic of this tunicate species and examine the genomic bases of the species? potential for adaptation, and specifically the role of flexible gene networks for successful responses to changing environmental conditions. The primary hypothesis driving this research is that predicted S. thompsoni orthologs (i.e., genes of the same function that share a common ancestor) that show evidence of rapid evolution are indicative of positive selection, and further that these genes and associated gene networks provide the basis for rapid adaptation of the Antarctic salp to environmental variation associated with a changing ocean. The proposed genome assembly strategy will allow further refinements and scaffolding of the current, highly fragmented genome assembly using the methods developed during previous work. Specimens of S. thompsoni now archived at UConn will be analyzed to improve the salp genome assembly, increasing overall scaffold length, and decreasing the number of total contigs. High-quality reference assemblies will be obtained with two high-output paired-end sequencing runs (Illumina) on a single individual, coupled with three runs on the Oxford Nanopore long-read sequencer. The same sequencing strategy will be performed on a sub-sampling of tissues from the same specimen to produce a very high quality reference transcriptome, which will allow for high quality gene models and near-complete gene predictions in the genome assembly. Comparisons with available genomic data for Urochordate and Cephalochordate species will increase the number of orthologs analyzed. Orthologous genes will be tested for evidence of rapid selection in the salp lineage, and the results will be compared to published expression profiles and ontology functions for the salp. All data will be made publicly available via existing web portals; a project website will be developed to disseminate research results for access by the both research and educational communities. Website design will use a local instance of jbrowse that will offer annotations, downloadable data files, and tracts of previously-published datasets.", "east": -53.74, "geometry": "POINT(-64.155 -64.085)", "instruments": null, "is_usap_dc": true, "keywords": "SHIPS; PELAGIC; Southern Ocean", "locations": "Southern Ocean", "north": -60.9, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Bucklin, Ann; O\u0027Neill, Rachel J", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e SHIPS", "repo": "BCO-DMO", "repositories": "BCO-DMO", "science_programs": null, "south": -67.27, "title": "Genome Assembly and Analysis of the Bloom Forming Southern Ocean Salp, Salpa thompsoni", "uid": "p0010224", "west": -74.57}, {"awards": "0231006 DeVries, Arthur; 1142158 Cheng, Chi-Hing", "bounds_geometry": "POLYGON((163 -76.5,163.5 -76.5,164 -76.5,164.5 -76.5,165 -76.5,165.5 -76.5,166 -76.5,166.5 -76.5,167 -76.5,167.5 -76.5,168 -76.5,168 -76.63,168 -76.76,168 -76.89,168 -77.02,168 -77.15,168 -77.28,168 -77.41,168 -77.54,168 -77.67,168 -77.8,167.5 -77.8,167 -77.8,166.5 -77.8,166 -77.8,165.5 -77.8,165 -77.8,164.5 -77.8,164 -77.8,163.5 -77.8,163 -77.8,163 -77.67,163 -77.54,163 -77.41,163 -77.28,163 -77.15,163 -77.02,163 -76.89,163 -76.76,163 -76.63,163 -76.5))", "dataset_titles": "High-resolution benthic seawater temperature record 1999-2012 (25-40m depth) from near intake jetty at McMurdo Station, Antarctica; Metadata associated with the description of Akarotaxis gouldae n. sp. (Bathydraconidae)", "datasets": [{"dataset_uid": "601275", "doi": null, "keywords": "Antarctica; Benthic; McMurdo Sound; Mcmurdo Station; Oceans; Physical Oceanography; Temperature Probe; Water Temperature", "people": "Devries, Arthur; Cziko, Paul; Cheng, Chi-Hing", "repository": "USAP-DC", "science_program": null, "title": "High-resolution benthic seawater temperature record 1999-2012 (25-40m depth) from near intake jetty at McMurdo Station, Antarctica", "url": "https://www.usap-dc.org/view/dataset/601275"}, {"dataset_uid": "601811", "doi": "10.15784/601811", "keywords": "Antarctica; Bellingshausen Sea; Cryosphere; Southern Ocean", "people": "Hilton, Eric; Steinberg, Deborah; Biesack, Ellen; Corso, Andrew; McDowell, Jan; Cheng, Chi-Hing; Desvignes, Thomas", "repository": "USAP-DC", "science_program": "LTER", "title": "Metadata associated with the description of Akarotaxis gouldae n. sp. (Bathydraconidae)", "url": "https://www.usap-dc.org/view/dataset/601811"}], "date_created": "Wed, 08 Apr 2020 00:00:00 GMT", "description": "Antarctic notothenioid fishes exhibit two adaptive traits to survive in frigid temperatures. The first of these is the production of anti-freeze proteins in their blood and tissues. The second is a system-wide ability to perform cellular and physiological functions at extremely cold temperatures.The proposal goals are to show how Antarctic fishes use these characteristics to avoid freezing, and which additional genes are turned on, or suppressed in order for these fishes to maintain normal physiological function in extreme cold temperatures. Progressively colder habitats are encountered in the high latitude McMurdo Sound and Ross Shelf region, along with somewhat milder near?shore water environments in the Western Antarctic Peninsula (WAP). By quantifying the extent of ice crystals invading and lodging in the spleen, the percentage of McMurdo Sound fish during austral summer (Oct-Feb) will be compared to the WAP intertidal fish during austral winter (Jul-Sep) to demonstrate their capability and extent of freeze avoidance. Resistance to ice entry in surface epithelia (e.g. skin, gill and intestinal lining) is another expression of the adaptation of these fish to otherwise lethally freezing conditions. The adaptive nature of a uniquely characteristic polar genome will be explored by the study of the transcriptome (the set of expressed RNA transcripts that constitutes the precursor to set of proteins expressed by an entire genome). Three notothenioid species (E.maclovinus, D. Mawsoni and C. aceratus) will be analysed to document evolutionary genetic changes (both gain and loss) shaped by life under extreme chronic cold. A differential gene expression (DGE) study will be carried out on these different species to evaluate evolutionary modification of tissue-wide response to heat challenges. The transcriptomes and other sequencing libraries will contribute to de novo ice-fish genome sequencing efforts.", "east": 168.0, "geometry": "POINT(165.5 -77.15)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD", "is_usap_dc": true, "keywords": "McMurdo Sound; MARINE ECOSYSTEMS; Water Temperature; AQUATIC SCIENCES; OCEAN TEMPERATURE; FIELD INVESTIGATION; USAP-DC", "locations": "McMurdo Sound", "north": -76.5, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Cheng, Chi-Hing; Devries, Arthur", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -77.8, "title": "Antarctic Notothenioid Fish Freeze Avoidance and Genome-wide Evolution for Life in the Cold", "uid": "p0010091", "west": 163.0}, {"awards": "1444167 Detrich, H. William", "bounds_geometry": "POLYGON((-70 -58,-68.5 -58,-67 -58,-65.5 -58,-64 -58,-62.5 -58,-61 -58,-59.5 -58,-58 -58,-56.5 -58,-55 -58,-55 -59.8,-55 -61.6,-55 -63.4,-55 -65.2,-55 -67,-55 -68.8,-55 -70.6,-55 -72.4,-55 -74.2,-55 -76,-56.5 -76,-58 -76,-59.5 -76,-61 -76,-62.5 -76,-64 -76,-65.5 -76,-67 -76,-68.5 -76,-70 -76,-70 -74.2,-70 -72.4,-70 -70.6,-70 -68.8,-70 -67,-70 -65.2,-70 -63.4,-70 -61.6,-70 -59.8,-70 -58))", "dataset_titles": "Assembled Contig Dat for Daane et al. (2019); E-MTAB-6759: RNA-seq across tissues in four Notothenioid species (Antarctic icefish); Expedition Data of LMG1603; Expedition Data of LMG1604; Expedition Data of LMG1605; Expedition Data of LMG1803; Expedition Data of LMG1804; Expedition Data of LMG1805; Full raw data set, computer code, and evolutionary trajectories for all species in Damsgaard et al. (2019); Histology-, CT-, ultrasound-, and MRI-scans (~2 TB) for Damsgaard et al. (2019); PRJNA420419: Genome and Transcriptome Data for Kim et al. (2019) Blackfin Icefish Genome; PRJNA531677: Sequencing Data for Daane et al. (2019); S-BSST132: Assembled Transcriptomes for Berthelot et al. (2018); SRP047484 RAD-tag Sequences of Genetically Mapped Notothenia coriiceps embryos; SRP118539: RAD-tag Sequences of Genetically Mapped Chaenocephalus aceratus Embryos; Transposable element sequences and genome sizes, refs 142597 to MF142757", "datasets": [{"dataset_uid": "200103", "doi": "", "keywords": null, "people": null, "repository": "GitHub", "science_program": null, "title": "Full raw data set, computer code, and evolutionary trajectories for all species in Damsgaard et al. (2019)", "url": "https://github.com/elifesciences-publications/Retinaevolution"}, {"dataset_uid": "200102", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Transposable element sequences and genome sizes, refs 142597 to MF142757", "url": "https://www.ncbi.nlm.nih.gov/nuccore?LinkName=pubmed_nuccore\u0026from_uid=29739320"}, {"dataset_uid": "200099", "doi": "10.5281/zenodo.2628936", "keywords": null, "people": null, "repository": "Zenodo", "science_program": null, "title": "Assembled Contig Dat for Daane et al. (2019)", "url": "https://zenodo.org/record/2628936#.Xegqj3dFw2w"}, {"dataset_uid": "200098", "doi": "", "keywords": null, "people": null, "repository": "NCBI BioProject", "science_program": null, "title": "PRJNA531677: Sequencing Data for Daane et al. (2019)", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA531677"}, {"dataset_uid": "200096", "doi": "", "keywords": null, "people": null, "repository": "NCBI SRA", "science_program": null, "title": "SRP047484 RAD-tag Sequences of Genetically Mapped Notothenia coriiceps embryos", "url": "https://www.ncbi.nlm.nih.gov/sra/?term=SRP047484"}, {"dataset_uid": "200095", "doi": "", "keywords": null, "people": null, "repository": "BioStudies", "science_program": null, "title": "S-BSST132: Assembled Transcriptomes for Berthelot et al. (2018)", "url": "https://www.ebi.ac.uk/biostudies/studies/S-BSST132"}, {"dataset_uid": "200093", "doi": "", "keywords": null, "people": null, "repository": "NCBI SRA", "science_program": null, "title": "SRP118539: RAD-tag Sequences of Genetically Mapped Chaenocephalus aceratus Embryos", "url": "https://www.ncbi.nlm.nih.gov/sra/SRP118539 "}, {"dataset_uid": "200094", "doi": "", "keywords": null, "people": null, "repository": "Array Express", "science_program": null, "title": "E-MTAB-6759: RNA-seq across tissues in four Notothenioid species (Antarctic icefish)", "url": "https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-6759/"}, {"dataset_uid": "200092", "doi": "", "keywords": null, "people": null, "repository": "NCBI BioProject", "science_program": null, "title": "PRJNA420419: Genome and Transcriptome Data for Kim et al. (2019) Blackfin Icefish Genome", "url": "https://www.ncbi.nlm.nih.gov/bioproject/?term=prjna420419"}, {"dataset_uid": "200253", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data of LMG1605", "url": "https://www.rvdata.us/search/cruise/LMG1605"}, {"dataset_uid": "200252", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data of LMG1604", "url": "https://www.rvdata.us/search/cruise/LMG1604"}, {"dataset_uid": "200104", "doi": "", "keywords": null, "people": null, "repository": "eLife", "science_program": null, "title": "Histology-, CT-, ultrasound-, and MRI-scans (~2 TB) for Damsgaard et al. (2019)", "url": "https://retinaevolution.bios.au.dk/eLife%20documentation/README.txt"}, {"dataset_uid": "200251", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data of LMG1804", "url": "https://www.rvdata.us/search/cruise/LMG1804"}, {"dataset_uid": "200250", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data of LMG1603", "url": "https://www.rvdata.us/search/cruise/LMG1603"}, {"dataset_uid": "200249", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data of LMG1803", "url": "https://www.rvdata.us/search/cruise/LMG1803"}, {"dataset_uid": "200254", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data of LMG1805", "url": "https://www.rvdata.us/search/cruise/LMG1805"}], "date_created": "Wed, 04 Dec 2019 00:00:00 GMT", "description": "Antarctic fish and their early developmental stages are an important component of the food web that sustains life in the cold Southern Ocean (SO) that surrounds Antarctica. They feed on smaller organisms and in turn are eaten by larger animals, including seals and killer whales. Little is known about how rising ocean temperatures will impact the development of Antarctic fish embryos and their growth after hatching. This project will address this gap by assessing the effects of elevated temperatures on embryo viability, on the rate of embryo development, and on the gene \"toolkits\" that respond to temperature stress. One of the two species to be studied does not produce red blood cells, a defect that may make its embryos particularly vulnerable to heat. The outcomes of this research will provide the public and policymakers with \"real world\" data that are necessary to inform decisions and design strategies to cope with changes in the Earth\u0027s climate, particularly with respect to protecting life in the SO. The project will also further the NSF goals of training new generations of scientists, including providing scientific training for undergraduate and graduate students, and of making scientific discoveries available to the general public. This includes the unique educational opportunity for undergraduates to participate in research in Antarctica and engaging the public in several ways, including the development of professionally-produced educational videos with bi-lingual closed captioning. Since the onset of cooling of the SO about 40 million years ago, evolution of Antarctic marine organisms has been driven by the development of cold temperatures. Because body temperatures of Antarctic fishes fall in a narrow range determined by their habitat (-1.9 to +2.0 C) they are particularly attractive models for understanding how organismal physiology and biochemistry have been shaped to maintain life in a cooling environment. The long-term objective of this project is to understand the capacities of Antarctic fishes to acclimatize and/or adapt to rapid oceanic warming through analysis of their underlying genetic \"toolkits.\" This objective will be accomplished through three Specific Aims: 1) assessing the effects of elevated temperatures on gene expression during development of embryos; 2) examining the effects of elevated temperatures on embryonic morphology and on the temporal and spatial patterns of gene expression; and 3) evaluating the evolutionary mechanisms that have led to the loss of the red blood cell genetic program by the white-blooded fishes. Aims 1 and 2 will be investigated by acclimating experimental embryos of both red-blooded and white-blooded fish to elevated temperatures. Differential gene expression will be examined through the use of high throughput RNA sequencing. The temporal and spatial patterns of gene expression in the context of embryonic morphology (Aim 2) will be determined by microscopic analysis of embryos \"stained\" with (hybridized to) differentially expressed gene probes revealed by Aim 1; other developmental marker genes will also be used. The genetic lesions resulting from loss of red blood cells by the white-blooded fishes (Aim 3) will be examined by comparing genes and genomes in the two fish groups.", "east": -55.0, "geometry": "POINT(-62.5 -67)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "NOT APPLICABLE; Polar; South Shetland Islands; USAP-DC; COASTAL", "locations": "Polar; South Shetland Islands", "north": -58.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Detrich, H. William", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "GitHub", "repositories": "Array Express; BioStudies; eLife; GitHub; NCBI BioProject; NCBI GenBank; NCBI SRA; R2R; Zenodo", "science_programs": null, "south": -76.0, "title": "Antarctic Notothenioid Fishes: Sentinel Taxa for Southern Ocean Warming", "uid": "p0010073", "west": -70.0}, {"awards": "1341393 Denlinger, David; 1341385 Lee, Richard", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "Alaskozetes antarcticus Raw sequence reads; Belgica antarctica Integrated Genome and Transcriptome Project; Data from: Rapid cold hardening protects against sublethal freezing injury in an Antarctic insect", "datasets": [{"dataset_uid": "200054", "doi": " https://doi.org/10.5061/dryad.29p7ng2", "keywords": null, "people": null, "repository": "Dryad", "science_program": null, "title": "Data from: Rapid cold hardening protects against sublethal freezing injury in an Antarctic insect", "url": "https://datadryad.org/resource/doi:10.5061/dryad.29p7ng2"}, {"dataset_uid": "200052", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Alaskozetes antarcticus Raw sequence reads", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA428758"}, {"dataset_uid": "200053", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Belgica antarctica Integrated Genome and Transcriptome Project", "url": "https://www.ncbi.nlm.nih.gov/bioproject/175916"}], "date_created": "Mon, 12 Aug 2019 00:00:00 GMT", "description": "Polar regions are deserts that are not only cold but also lack access to free water. Antarctic insects have unique survival mechanisms including the ability to tolerate freezing and extensive dehydration, surviving the loss of 70% of their body water. How this is done is of interest not only for understanding seasonal adaptations of insects and how they respond to climate change, but the molecular and physiological mechanisms employed may offer valuable insights into more general mechanisms that might be exploited for cryopreservation and long-term storage of human tissues and organs for transplantation and other medical applications. The investigators will study the proteins that are responsible for removing water from the body, cell level consequences of this, and how the responsible genes vary between populations. The project will also further the NSF goals of making scientific discoveries available to the general public and of training new generations of scientists. Each year a K-12 teacher will be a member of the field team and assist with fieldwork and outreach to school children and their teachers. Educational outreach efforts include presentations at local schools and national teacher meetings, providing lesson plans and podcasts on a website, and continuing to publish articles related to this research in education journals. In addition, undergraduate and graduate students will receive extensive training in all aspects of the research project with extended experiences that include publication of scientific papers and presentations at national meetings. This project focuses on deciphering the physiological and molecular mechanisms that enable the Antarctic midge Belgica antarctica to survive environmental stress and the loss of most of its body water in the desiccating polar environment. This extremophile is an ideal system for investigating mechanisms of stress tolerance and local geographic adaptations and its genome has recently been sequenced. This project has three focal areas: 1) Evaluating the role of aquaporins (water channel proteins) in the rapid removal of water from the body by studying expression of their genes during dehydration; 2) Investigating the mechanism of metabolic depression and the role of autophagy (controlled breakdown of cellular components) as a mediator of stress tolerance by studying expression of the genes responsible for autophagy during the dehydration process; and 3) Evaluating the population structure, gene flow, and adaptive variation in physiological traits associated with stress tolerance using a genetic approach that takes advantage of the genomic sequence available for this species coupled with physiological and environmental data from the sampled populations and their habitats.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "Antarctica; USAP-DC; ARTHROPODS; NOT APPLICABLE", "locations": "Antarctica", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Denlinger, David; Lee, Richard", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "Dryad", "repositories": "Dryad; NCBI GenBank", "science_programs": null, "south": -90.0, "title": "Collaborative Research: Winter Survival Mechanisms and Adaptive Genetic Variation in an Antarctic Insect", "uid": "p0010048", "west": -180.0}, {"awards": "1247510 Detrich, H. William", "bounds_geometry": null, "dataset_titles": "E-MTAB-6759: RNA-seq across tissues in four Notothenioid species (Antarctic icefish); Expedition Data; Expedition data of LMG1003; Expedition data of LMG1004; PRJNA420419: Chaenocephalus aceratus Genome sequencing; PRJNA66471: Notothenia coriiceps Genome Sequencing Notothenia coriiceps isolate:Sejong01 (black rockcod); S-BSST 132: Assembled Transcriptomes for Berthelot et al. (2018); SRA091269: Notothenia coriiceps RNA Raw Sequence Reads; SRP047484: RAD-tag Sequences of Genetically Mapped Notothenia coriiceps Embryos ; SRP118539: RAD-tag Sequences of Genetically Mapped Chaenocephalus aceratus Embryos", "datasets": [{"dataset_uid": "200144", "doi": "", "keywords": null, "people": null, "repository": "Array Express", "science_program": null, "title": "E-MTAB-6759: RNA-seq across tissues in four Notothenioid species (Antarctic icefish)", "url": "https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-6759/"}, {"dataset_uid": "200145", "doi": "", "keywords": null, "people": null, "repository": "BioStudies", "science_program": null, "title": "S-BSST 132: Assembled Transcriptomes for Berthelot et al. (2018)", "url": "https://www.ebi.ac.uk/biostudies/studies/S-BSST132"}, {"dataset_uid": "200146", "doi": "", "keywords": null, "people": null, "repository": "NCBI SRA", "science_program": null, "title": "SRP047484: RAD-tag Sequences of Genetically Mapped Notothenia coriiceps Embryos ", "url": "https://www.ncbi.nlm.nih.gov/sra/?term=SRP047484"}, {"dataset_uid": "002684", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG1003", "url": "https://www.rvdata.us/search/cruise/LMG1003"}, {"dataset_uid": "002685", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG1004", "url": "https://www.rvdata.us/search/cruise/LMG1004"}, {"dataset_uid": "001508", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0807"}, {"dataset_uid": "001509", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0806"}, {"dataset_uid": "200093", "doi": "", "keywords": null, "people": null, "repository": "NCBI SRA", "science_program": null, "title": "SRP118539: RAD-tag Sequences of Genetically Mapped Chaenocephalus aceratus Embryos", "url": "https://www.ncbi.nlm.nih.gov/sra/SRP118539 "}, {"dataset_uid": "200026", "doi": "", "keywords": null, "people": null, "repository": "NCBI SRA", "science_program": null, "title": "SRA091269: Notothenia coriiceps RNA Raw Sequence Reads", "url": "https://www.ncbi.nlm.nih.gov/sra/?term=SRA091269"}, {"dataset_uid": "200142", "doi": "", "keywords": null, "people": null, "repository": "NCBI BioProject", "science_program": null, "title": "PRJNA66471: Notothenia coriiceps Genome Sequencing Notothenia coriiceps isolate:Sejong01 (black rockcod)", "url": "https://www.ncbi.nlm.nih.gov/bioproject/66471"}, {"dataset_uid": "200143", "doi": "", "keywords": null, "people": null, "repository": "NCBI BioProject", "science_program": null, "title": "PRJNA420419: Chaenocephalus aceratus Genome sequencing", "url": "https://www.ncbi.nlm.nih.gov/bioproject/420419"}], "date_created": "Mon, 08 Apr 2019 00:00:00 GMT", "description": "Since the advent of Antarctic continental glaciation, the opening of the Drake Passage between South America and the Antarctic Peninsula, and the onset of cooling of the Southern Ocean ~40-25 million years ago, evolution of the Antarctic marine biota has been driven by the development of extreme cold temperatures. As circum-Antarctic coastal temperatures declined during this period from ~20\u00b0C to the modern ?1.9 to +2.0\u00b0C (reached ~8-10 million years ago), the psychrophilic (cold-loving) ectotherms of the Southern Ocean evolved compensatory molecular, cellular, and physiological traits that enabled them to maintain normal metabolic function at cold temperatures. Today, these organisms are threatened by rapid warming of the Southern Ocean over periods measured in centuries (as much as 5\u00b0C/100 yr), a timeframe so short that re-adaptation and/or acclimatization to the ?new warm? may not be possible. Thus, the long-term goals of this research project are: 1) to understand the biochemical and physiological capacities of the embryos of Antarctic notothenioid fish to resist or compensate for rapid oceanic warming; and 2) to assess the genetic toolkit available to support the acclimatization and adaptation of Antarctic notothenioid embryos to their warming habitat. The specific aims of this work are: 1) to determine the capacity of the chaperonin complex of notothenioid fishes to assist protein folding at temperatures between ?4 and +20\u00b0C; and 2) to evaluate the genetic responses of notothenioid embryos, measured as global differential gene transcription, to temperature challenge, with ?1.9\u00b0C as the ?normal? control and +4 and +10\u00b0C as high temperature insults. The physiology of embryonic development of marine stenotherms under future climate change scenarios is an important but understudied problem. This project will provide valuable insights into the capacity of Antarctic fish embryos to acclimatize and adapt to plausible climate change scenarios by examining multiple levels of biological organization, from the biochemical to the organismal. The results should also be broadly applicable to understanding the impact of global warming on marine biota worldwide. The research will also introduce graduate and REU undergraduate students to state-of-the-art biochemical, cellular, and molecular-biological research relevant to ecological and environmental issues of the Antarctic marine ecosystem.", "east": null, "geometry": null, "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e SAMPLERS \u003e TRAWLS/NETS \u003e BOTTOM TRAWL", "is_usap_dc": false, "keywords": "AQUATIC SCIENCES; R/V LMG; USAP-DC; Southern Ocean", "locations": "Southern Ocean", "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Detrich, H. William", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "Array Express", "repositories": "Array Express; BioStudies; NCBI BioProject; NCBI SRA; R2R", "science_programs": null, "south": null, "title": "Protein Folding and Embryogenesis in Antarctic Fishes: A Comparative Approach to Environmental Stress", "uid": "p0010024", "west": null}, {"awards": "1341479 Marchetti, Adrian", "bounds_geometry": "POLYGON((-72.8 -48,-67.12 -48,-61.44 -48,-55.76 -48,-50.08 -48,-44.4 -48,-38.72 -48,-33.04 -48,-27.36 -48,-21.68 -48,-16 -48,-16 -50.02,-16 -52.04,-16 -54.06,-16 -56.08,-16 -58.1,-16 -60.12,-16 -62.14,-16 -64.16,-16 -66.18,-16 -68.2,-21.68 -68.2,-27.36 -68.2,-33.04 -68.2,-38.72 -68.2,-44.4 -68.2,-50.08 -68.2,-55.76 -68.2,-61.44 -68.2,-67.12 -68.2,-72.8 -68.2,-72.8 -66.18,-72.8 -64.16,-72.8 -62.14,-72.8 -60.12,-72.8 -58.1,-72.8 -56.08,-72.8 -54.06,-72.8 -52.04,-72.8 -50.02,-72.8 -48))", "dataset_titles": "16S and 18S Sequence data; Fragilariopsis kerguelensis iron and light transcriptomes; Physiology and transcriptomes of polar isolates; Polar isolate transcriptomes; Sequence data from Ocean Station Papa seawater ; Sequence data RNA-Seq of marine phytoplankton: FeB12", "datasets": [{"dataset_uid": "200016", "doi": "", "keywords": null, "people": null, "repository": "iMicrobe", "science_program": null, "title": "Fragilariopsis kerguelensis iron and light transcriptomes", "url": "https://www.imicrobe.us/#/projects/104"}, {"dataset_uid": "200020", "doi": "", "keywords": null, "people": null, "repository": "BCO-DMO", "science_program": null, "title": "Physiology and transcriptomes of polar isolates", "url": "https://www.bco-dmo.org/project/653229"}, {"dataset_uid": "200019", "doi": "", "keywords": null, "people": null, "repository": "Cyverse Data Commons", "science_program": null, "title": "Polar isolate transcriptomes", "url": "http://datacommons.cyverse.org/search/?search_term=unc_phyto_isolates"}, {"dataset_uid": "200018", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Sequence data from Ocean Station Papa seawater ", "url": "https://www.ncbi.nlm.nih.gov/sra/SRP006906"}, {"dataset_uid": "200021", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "16S and 18S Sequence data", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA299401"}, {"dataset_uid": "200017", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Sequence data RNA-Seq of marine phytoplankton: FeB12", "url": "https://www.ncbi.nlm.nih.gov/sra/SRP074366"}], "date_created": "Mon, 11 Mar 2019 00:00:00 GMT", "description": "The Southern Ocean surrounding Antarctica is changing rapidly in response to Earth\u0027s warming climate. These changes will undoubtedly influence communities of primary producers (the organisms at the base of the food chain, particularly plant-like organisms using sunlight for energy) by altering conditions that influence their growth and composition. Because primary producers such as phytoplankton play an important role in global biogeochemical cycling, it is essential to understand how they will respond to changes in their environment. The growth of phytoplankton in certain regions of the Southern Ocean is constrained by steep gradients in chemical and physical properties that vary in both space and time. Light and iron have been identified as key variables influencing phytoplankton abundance and distribution within Antarctic waters. Microscopic algae known as diatoms are dominant members of the phytoplankton and sea ice communities, accounting for significant proportions of primary production. The overall objective of this project is to identify the molecular bases for the physiological responses of polar diatoms to varying light and iron conditions. The project should provide a means of evaluating the extent these factors regulate diatom growth and influence net community productivity in Antarctic waters. The project will also further the NSF goals of making scientific discoveries available to the general public and of training new generations of scientists. It will facilitate the teaching and learning of polar-related topics by translating the research objectives into readily accessible educational materials for middle-school students. This project will also provide funding to enable a graduate student and several undergraduate students to be trained in the techniques and perspectives of modern biology. Although numerous studies have investigated how polar diatoms are affected by varying light and iron, the cellular mechanisms leading to their distinct physiological responses remain unknown. Using comparative transcriptomics, the expression patterns of key genes and metabolic pathways in several ecologically important polar diatoms recently isolated from Antarctic waters and grown under varying iron and irradiance conditions will be examined. In addition, molecular indicators for iron and light limitation will be developed within these polar diatoms through the identification of iron- and light-responsive genes -- the expression patterns of which can be used to determine their physiological status. Upon verification in laboratory cultures, these indicators will be utilized by way of metatranscriptomic sequencing to examine iron and light limitation in natural diatom assemblages collected along environmental gradients in Western Antarctic Peninsula waters. In order to fully understand the role phytoplankton play in Southern Ocean biogeochemical cycles, dependable methods that provide a means of elucidating the physiological status of phytoplankton at any given time and location are essential.", "east": -16.0, "geometry": "POINT(-44.4 -58.1)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "NOT APPLICABLE; AQUATIC SCIENCES; PHYTOPLANKTON; USAP-DC; Southern Ocean; Sea Surface; DIATOMS", "locations": "Sea Surface; Southern Ocean", "north": -48.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Marchetti, Adrian", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "iMicrobe", "repositories": "BCO-DMO; Cyverse Data Commons; iMicrobe; NCBI GenBank", "science_programs": null, "south": -68.2, "title": "Iron and Light Limitation in Ecologically Important Polar Diatoms: Comparative Transcriptomics and Development of Molecular Indicators", "uid": "p0010018", "west": -72.8}, {"awards": "1656344 Bowman, Jeff", "bounds_geometry": "POLYGON((-64.1 -64.75,-64.08 -64.75,-64.06 -64.75,-64.04 -64.75,-64.02 -64.75,-64 -64.75,-63.98 -64.75,-63.96 -64.75,-63.94 -64.75,-63.92 -64.75,-63.9 -64.75,-63.9 -64.775,-63.9 -64.8,-63.9 -64.825,-63.9 -64.85,-63.9 -64.875,-63.9 -64.9,-63.9 -64.925,-63.9 -64.95,-63.9 -64.975,-63.9 -65,-63.92 -65,-63.94 -65,-63.96 -65,-63.98 -65,-64 -65,-64.02 -65,-64.04 -65,-64.06 -65,-64.08 -65,-64.1 -65,-64.1 -64.975,-64.1 -64.95,-64.1 -64.925,-64.1 -64.9,-64.1 -64.875,-64.1 -64.85,-64.1 -64.825,-64.1 -64.8,-64.1 -64.775,-64.1 -64.75))", "dataset_titles": "\r\nMetadata accompanying BioProject SUB4579142 ; Western Antarctic Peninsula Marine Metatranscriptomes Sep 29 2018", "datasets": [{"dataset_uid": "601153", "doi": "", "keywords": "Antarctic; Antarctica; Bacteria; Bacteria Production; Biota; Chlorophyll; LTER Palmer Station; Primary Production; Sea Ice; Southern Ocean", "people": "Bowman, Jeff", "repository": "USAP-DC", "science_program": null, "title": "\r\nMetadata accompanying BioProject SUB4579142 ", "url": "https://www.usap-dc.org/view/dataset/601153"}, {"dataset_uid": "200010", "doi": "", "keywords": null, "people": null, "repository": "NCBI SRA", "science_program": null, "title": "Western Antarctic Peninsula Marine Metatranscriptomes Sep 29 2018", "url": "https://submit.ncbi.nlm.nih.gov/subs/sra/SUB4579142/overview"}], "date_created": "Thu, 31 Jan 2019 00:00:00 GMT", "description": "This EAGER project will compare gene expression patterns in the planktonic communities under ice covers that form in coastal embayment\u0027s in the Antarctic Peninsula. Previous efforts taking advantage of unique ice conditions in November and December of 2015 allowed researchers to conduct an experiment to examine the role of sea ice cover on microbial carbon and energy transfer during the winter-spring transition. The EAGER effort will enable the researchers to conduct the \"omics\" analyses of the phytoplankton to determine predominant means by which energy is acquired and used in these settings. This EAGER effort will apply new expertise to fill an existing gap in ecological observations along the West Antarctic Peninsula. The principle product of the proposed work will be a novel dataset to be analyzed and by an early career researcher from an underserved community (veteran). The critical baseline data contained in this dataset enable a comparison of eukaryotic and prokaryotic gene expression patterns to establish the relative importance of chemoautotrophy, heterotrophy, mixotrophy, and phototrophy during the experiments. this information and data will be made immediately available to the broader scientific community, and will enable the development of further hypotheses on ecosystem change as sea ice cover changes in the region. Very little gene expression data is currently available for the Antarctic marine environment, and no gene expression data is available during the ecologically critical winter to spring transition. Moreover, ice cover in bays is common along the West Antarctic Peninsula yet the opportunity to study cryptophyte phytoplankton physiology beneath such ice conditions in coastal embayments is rare.", "east": -63.9, "geometry": "POINT(-64 -64.875)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "USAP-DC; NOT APPLICABLE; Antarctica; COASTAL", "locations": "Antarctica", "north": -64.75, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Bowman, Jeff", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "USAP-DC", "repositories": "NCBI SRA; USAP-DC", "science_programs": null, "south": -65.0, "title": "A Preliminary Assessment of the Influence of Ice Cover on Microbial Carbon and Energy Acquisition during the Antarctic Winter-spring Seasonal Transition", "uid": "p0010003", "west": -64.1}, {"awards": "1341362 Gast, Rebecca", "bounds_geometry": "POLYGON((-180 -65,-176 -65,-172 -65,-168 -65,-164 -65,-160 -65,-156 -65,-152 -65,-148 -65,-144 -65,-140 -65,-140 -66.5,-140 -68,-140 -69.5,-140 -71,-140 -72.5,-140 -74,-140 -75.5,-140 -77,-140 -78.5,-140 -80,-144 -80,-148 -80,-152 -80,-156 -80,-160 -80,-164 -80,-168 -80,-172 -80,-176 -80,180 -80,178 -80,176 -80,174 -80,172 -80,170 -80,168 -80,166 -80,164 -80,162 -80,160 -80,160 -78.5,160 -77,160 -75.5,160 -74,160 -72.5,160 -71,160 -69.5,160 -68,160 -66.5,160 -65,162 -65,164 -65,166 -65,168 -65,170 -65,172 -65,174 -65,176 -65,178 -65,-180 -65))", "dataset_titles": "Dinoflagellate sequende data", "datasets": [{"dataset_uid": "000240", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Dinoflagellate sequende data", "url": "http://www.ncbi.nlm.nih.gov/bioproject/428208"}], "date_created": "Mon, 12 Feb 2018 00:00:00 GMT", "description": "Kleptoplasty, the temporary acquisition and use of functional chloroplasts derived from algal prey, is viewed as an important model for the early evolution of the permanent, endosymbiotically-derived chloroplasts found in all permanently photosynthetic eukaryotes. This project will study the evolutionary history and expression of plastid-targeted genes in an abundant Antarctic dinoflagellate that steals chloroplasts from an ecologically important alga, the haptophyte Phaeocystis. Algae play an important role in the fixation and export of CO2 in the Southern Ocean, and this project will explore the genetic basis for the function of these chimeric cells with regard to their functional adaptation to extreme environments and will study the evolutionary history and expression of plastid-targeted genes in both the host and recipient. The project seeks to determine whether the kleptoplastidic dinoflagellate utilizes ancestral plastid proteins to regulate its stolen plastid, and how their transcription is related to environmental factors that are relevant to the Southern Ocean environment (temperature and light). To accomplish these goals, the project will utilize high throughput transcriptome analysis and RNA-sequencing experiments with the dinoflagellate and Phaeocystis. This work will help biologists understand the environmental success of this alternative nutritional strategy, and to assess the potential impact of anthropogenic climate change on the organism. The project will also contribute to the maintenance of a culture collection of heterotrophic, phototrophic and mixotrophic Antarctic protists that are available to the scientific community, and it will support the mentoring of a graduate student and a postdoctoral fellow. The work is being accomplished as an international collaboration between US and Canadian scientists, and in addition to publishing results in peer-reviewed journals, the investigators will incorporate aspects of this work into public outreach activities. These include field data analysis opportunities for middle school students and science-based art projects with local schools and museums.", "east": -140.0, "geometry": "POINT(-170 -72.5)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "NOT APPLICABLE; USAP-DC", "locations": null, "north": -65.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Gast, Rebecca", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "NCBI GenBank", "repositories": "NCBI GenBank", "science_programs": null, "south": -80.0, "title": "You are what you eat: The Role of Kleptoplasty in an Antarctic Dinoflagellate", "uid": "p0000302", "west": 160.0}, {"awards": "1044982 Bucklin, Ann", "bounds_geometry": "POLYGON((-69.3804 -52.760597,-67.79698 -52.760597,-66.21356 -52.760597,-64.63014 -52.760597,-63.04672 -52.760597,-61.4633 -52.760597,-59.87988 -52.760597,-58.29646 -52.760597,-56.71304 -52.760597,-55.12962 -52.760597,-53.5462 -52.760597,-53.5462 -53.9928073,-53.5462 -55.2250176,-53.5462 -56.4572279,-53.5462 -57.6894382,-53.5462 -58.9216485,-53.5462 -60.1538588,-53.5462 -61.3860691,-53.5462 -62.6182794,-53.5462 -63.8504897,-53.5462 -65.0827,-55.12962 -65.0827,-56.71304 -65.0827,-58.29646 -65.0827,-59.87988 -65.0827,-61.4633 -65.0827,-63.04672 -65.0827,-64.63014 -65.0827,-66.21356 -65.0827,-67.79698 -65.0827,-69.3804 -65.0827,-69.3804 -63.8504897,-69.3804 -62.6182794,-69.3804 -61.3860691,-69.3804 -60.1538588,-69.3804 -58.9216485,-69.3804 -57.6894382,-69.3804 -56.4572279,-69.3804 -55.2250176,-69.3804 -53.9928073,-69.3804 -52.760597))", "dataset_titles": "Expedition Data", "datasets": [{"dataset_uid": "001445", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG1110"}], "date_created": "Wed, 17 Jun 2015 00:00:00 GMT", "description": "The proposed research targets the molecular genetics of salps, an increasingly important member of the zooplankton in Antarctic waters who\u0027s dominance appears to be related to climate warming and sea ice loss. Specifically the research will examine genome-wide patterns of gene expression, target gene expression levels, and patterns of population genetic diversity and structure underlying the complex life history and population dynamics of S. thompsoni. The P.I.s hypothesize that (1) deep analysis of the Salpa thompsoni transcriptome will reveal significant associations among selected set of differentially expressed genes and critical life history stages and events (e.g., ontogenetic maturation, sexual reproduction, senescence) of the salp; and 2) the species will show variable levels of clonal diversity and significant genetic differentiation among populations in different regions of the Southern Ocean. Broader impacts include training of two graduate students; inclusion of undergraduates in research, and in a formal training workshop; development of a summer workshop for high school teachers in collaboration with Connecticut Sea Grant; and public outreach via postings on the Census of Marine Zooplankton homepage.", "east": -53.5462, "geometry": "POINT(-61.4633 -58.9216485)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; IN SITU/LABORATORY INSTRUMENTS \u003e CHEMICAL METERS/ANALYZERS \u003e FLUOROMETERS; IN SITU/LABORATORY INSTRUMENTS \u003e RECORDERS/LOGGERS \u003e AWS; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e XBT; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ADCP; EARTH REMOTE SENSING INSTRUMENTS \u003e ACTIVE REMOTE SENSING \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ECHO SOUNDERS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e THERMOSALINOGRAPHS", "is_usap_dc": false, "keywords": "PLANKTON; Antarctic Peninsula; R/V LMG", "locations": "Antarctic Peninsula", "north": -52.760597, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Bucklin, Ann", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "R2R", "repositories": "R2R", "science_programs": null, "south": -65.0827, "title": "Population ecology of Salpa thompsoni based on molecular indicators", "uid": "p0000508", "west": -69.3804}, {"awards": "0944557 Marsh, Adam", "bounds_geometry": "POINT(166 78)", "dataset_titles": "Environmental Genomics of an Antarctic Polychaete #SRP040946", "datasets": [{"dataset_uid": "000223", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Environmental Genomics of an Antarctic Polychaete #SRP040946", "url": "https://www.ncbi.nlm.nih.gov/sra/?term=SRP040946"}], "date_created": "Tue, 13 Jan 2015 00:00:00 GMT", "description": "Genome-enabled biology provides a foundation for understanding the genetic basis of organism-environment interactions. . The research project links gene expression, genome methylation, and metabolic rates to assess the mechanisms of environmental adaptation (temperature) across multiple generations in a polar, and closely related temperate, polychaete. By comparing these two species, the research will assess how a polar environment shapes responses to environmental stress. This work will produce: 1) a database of full transcriptome (gene specific) profiling data for the polar polychaete cultured at two temperatures; 2) the contribution of genome methylation to the suppression of gene transcription activities; 3) the linkage between shifts in mRNA pools and total cellular activities (as ATP consumption via respiration); 4) an assessment of the inheritance of patterns of gene expression and metabolic activities across three generations; and 5) a simple demographic model of the polar polychaete population dynamics under normal and \u0027global-warming\u0027 temperature scenarios. Broader impacts include two outreach activities. The first is a mentoring program, where African-American undergraduate students spend 1.5 years working on a research project with a UD faculty member (2 summers plus their senior academic year). The second is a children\u0027s display activity at UD?s School of Marine Science \"Coast Day\".", "east": 166.0, "geometry": "POINT(166 -78)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -78.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Marsh, Adam G.", "platforms": "Not provided", "repo": "NCBI GenBank", "repositories": "NCBI GenBank", "science_programs": null, "south": -78.0, "title": "Environmental Genomics in an Antarctic polychaete", "uid": "p0000355", "west": 166.0}, {"awards": "0632389 Murray, Alison; 0632278 Ducklow, Hugh", "bounds_geometry": "POLYGON((-77 -62,-75.5 -62,-74 -62,-72.5 -62,-71 -62,-69.5 -62,-68 -62,-66.5 -62,-65 -62,-63.5 -62,-62 -62,-62 -62.7,-62 -63.4,-62 -64.1,-62 -64.8,-62 -65.5,-62 -66.2,-62 -66.9,-62 -67.6,-62 -68.3,-62 -69,-63.5 -69,-65 -69,-66.5 -69,-68 -69,-69.5 -69,-71 -69,-72.5 -69,-74 -69,-75.5 -69,-77 -69,-77 -68.3,-77 -67.6,-77 -66.9,-77 -66.2,-77 -65.5,-77 -64.8,-77 -64.1,-77 -63.4,-77 -62.7,-77 -62))", "dataset_titles": "IPY: Bacterioplankton Genomic Adaptations to Antarctic Winter", "datasets": [{"dataset_uid": "600061", "doi": "10.15784/600061", "keywords": "Antarctica; Antarctic Peninsula; Biota; Chemistry:fluid; Chemistry:Fluid; Oceans; Southern Ocean", "people": "Grzymski, Joseph; Murray, Alison", "repository": "USAP-DC", "science_program": null, "title": "IPY: Bacterioplankton Genomic Adaptations to Antarctic Winter", "url": "https://www.usap-dc.org/view/dataset/600061"}], "date_created": "Thu, 08 Jul 2010 00:00:00 GMT", "description": "The Western Antarctic Peninsula is experiencing one of the most rapid rates of climate warming on Earth, with an increase of 5degrees C in the mean winter temperature in 50 years. Impacts on upper trophic levels are evident, though there have been few, if any studies that have considered the impacts on bacterioplankton in the Southern Ocean. This proposal will characterize the winter bacterioplankton genome, transcriptome, and proteome and discover those features (community composition, genes up-regulated, and proteins expressed) that are essential to winter bacterioplankton survival and livelihood. We have assembled a polar ocean ecology and genomics network including strategic partnerships with Palmer LTER, the British Antarctic Survey\u0027s ocean metagenome program, US and Canadian scientists studying the Arctic Ocean genome, an Australian colleague who specialized in archaeal proteomics, and French colleagues studying Sub-Antarctic and Coastal Adelie Land marine bacterioplankton. The primary objectives of this program are: 1 Describe the differences in diversity and genomic content between austral winter and summer bacterioplankton communities. 2. Investigate the winter-time bacterioplankton growth and cellular signals (mRNA and proteins expressed) in order to understand the specific adaptations key to survival. \u003cbr/\u003e\u003cbr/\u003eOur results will extend from the Antarctic to the Arctic - as the cold, dark, carbon-limited deep seas linking these two systems have many common features. Education and outreach activities target (i) undergraduate and graduate students, hopefully including minority students recruited through the Diversity in Research in Environmental and Marine Sciences (DREAMS) Program at VIMS; (ii) a broad audience with our education and outreach partnerships with The Cousteau Society and with the Census for Antarctic Marine Life program. Data and links to external databases will be listed on the http://genex2.dri.edu website. Sequence data will be publicly accessible in GenBank and IMG-M databases.", "east": -62.0, "geometry": "POINT(-69.5 -65.5)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -62.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Murray, Alison; Grzymski, Joseph; Ducklow, Hugh", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -69.0, "title": "IPY: Bacterioplankton Genomic Adaptations to Antarctic Winter", "uid": "p0000091", "west": -77.0}]

View results on map Help on results map