USAP-DC

{"dp_type": "Project", "free_text": "Dragonfish"}

[{"awards": "2232891 Postlethwait, John", "bounds_geometry": "POLYGON((-180 -37,-144 -37,-108 -37,-72 -37,-36 -37,0 -37,36 -37,72 -37,108 -37,144 -37,180 -37,180 -42.3,180 -47.6,180 -52.9,180 -58.2,180 -63.5,180 -68.8,180 -74.1,180 -79.4,180 -84.69999999999999,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -84.7,-180 -79.4,-180 -74.1,-180 -68.8,-180 -63.5,-180 -58.2,-180 -52.9,-180 -47.6,-180 -42.300000000000004,-180 -37))", "dataset_titles": null, "datasets": null, "date_created": "Mon, 14 Aug 2023 00:00:00 GMT", "description": "Antarctic animals face tremendous threats as Antarctic ice sheets melt and temperatures rise. About 34 million years ago, when Antarctica began to cool, most species of fish became locally extinct. A group called the notothenioids, however, survived due to the evolution of antifreeze. The group eventually split into over 120 species. Why did this group of Antarctic fishes evolve into so many species? One possible reason why a single population splits into two species relates to sex genes and sex chromosomes. Diverging species often have either different sex determining genes (genes that specify whether an individual\u2019s gonads become ovaries or testes) or have different sex chromosomes (chromosomes that differ between males and females within a species, like the human X and Y chromosomes). We know the sex chromosomes of only a few notothenioid species and know the genetic basis for sex determination in none of them. The aims of this research are to: 1) identify sex chromosomes in species representing every major group of Antarctic notothenioid fish; 2) discover possible sex determining genes in every major group of Antarctic notothenioid fish; and 3) find sex chromosomes and possible sex determining genes in two groups of temperate, warmer water, notothenioid fish. These warmer water fish include groups that never experienced the frigid Southern Ocean and groups that had ancestors inhabiting Antarctic oceans that later adjusted to warmer waters. This project will help explain the mechanisms that led to the division of a group of species threatened by climate change. This information is critical to conserve declining populations of Antarctic notothenioids, which are major food sources for other Antarctic species such as bird and seals. The project will offer a diverse group of undergraduates the opportunity to develop a permanent exhibit at the Eugene Science Center Museum. The exhibit will describe the Antarctic environment and explain its rapid climate change. It will also introduce the continent\u2019s bizarre fishes that live below the freezing point of water. The project will collaborate with the university\u2019s Science and Comics Initiative and students in the English Department\u2019s Comics Studies Minor to prepare short graphic novels explaining Antarctic biogeography, icefish specialties, and the science of this project as it develops. As Antarctica cooled, most species disappeared from the continent\u2019s waters, but cryonotothenioid fish radiated into a species flock. What facilitated this radiation? Coyne\u2019s \u201ctwo rules of speciation\u201d offer explanations for why species diverge: 1) the dysgenic sex in an interspecies hybrid is the one with two different sex chromosomes (i.e., in humans, it would be XY males and not XX females); and 2) \u201csex chromosomes play an outsized role in speciation\u201d. These ideas propel the project\u2019s main hypothesis: new sex chromosomes and new sex determination genes associate with cryonotothenioid speciation events. The main objective of the research is to identify notothenioid sex chromosomes and candidate sex-determination genes in many notothenioid species. The project\u2019s first aim is to identify Antarctic fish sex chromosomes, asking the question: Did new sex chromosomes accompany speciation events? Knowledge gaps include: which species have cryptic sex chromosomes; which have newly evolved sex chromosomes; and which are chromosomally XX/XY or ZZ/ZW. Methods involve population genomics (RAD-seq and Pool-seq) for more than 20 Antarctic cryonotothenioids. The prediction is frequent turnover of sex chromosomes. The project\u2019s second aim is to Identify candidate Antarctic cryonotothenioid sex-determination genes, asking the question: Did new sex-determination genes accompany Antarctic cryonotothenioid speciation events? A knowledge gap is the identity of sex determination genes in any notothenioid. Preliminary data show that three sex-linked loci are in or adjacent to three different candidate sex determination genes: 1) a duplicate of bmpr1ba in blackfin icefish; 2) a tandem duplicate of gsdf in South Georgia icefish; and 3) a transposed duplicate of gsdf in striped notothen. Methods involve annotating the genomic neighborhoods of cryonotothenioid sex linked loci for anomalies in candidate sex genes, sequencing sex chromosomes, and testing sex gene variants by CRISPR mutagenesis in zebrafish. The prediction is frequent turnover of sex determination genes. The project\u2019s third aim is to identify sex chromosomes and sex-determination genes in temperate notothenioids. Basally diverging temperate notothenioids (\u2018basals\u2019) lack identifiable sex chromosomes, consistent with temperature-cued sex determination, and one \u2018basal\u2019 species is a hermaphrodite. The constantly cold Southern Ocean rules out temperature, a common sex determination cue in many temperate fish, favoring genetic sex determination. Some cryonotothenioids re-invaded temperate waters (\u2018returnees\u2019). Knowledge gaps include whether basals and returnees have strong sex determination genes. Methods employ pool-seq. The prediction is that genetic sex determination is weak in basals and that returnees have the same, but weaker, sex-linked loci as their Antarctic sister clade. A permanent exhibit will be established at the Eugene Science Center Museum tentatively entitled: The Antarctic: its fishes and climate change. Thousands of visitors, especially school children will be exposed, to the science of Antarctic ecosystems and the impacts of climate change. The research team will collaborate with the university\u2019s Science and Comics Initiative to produce short graphic novels explaining Antarctic biogeography, icefish specialties, and this project. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "Speciation; Southern Ocean; Dragonfish; Antarctica; Plunderfish; Fish; Notothenioid; FISH; Eleginopsioidea; Icefish; MARINE ECOSYSTEMS; Cryonotothenioid; Sub-Antarctic", "locations": "Antarctica; Southern Ocean; Sub-Antarctic", "north": -37.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Postlethwait, John; Desvignes, Thomas", "platforms": null, "repositories": null, "science_programs": null, "south": -90.0, "title": "ANT LIA: The Role of Sex Determination in the Radiation of Antarctic Notothenioid Fish", "uid": "p0010431", "west": -180.0}, {"awards": "2232891 Postlethwait, John; 1947040 Postlethwait, John; 1543383 Postlethwait, John", "bounds_geometry": "POLYGON((-180 -37,-144 -37,-108 -37,-72 -37,-36 -37,0 -37,36 -37,72 -37,108 -37,144 -37,180 -37,180 -42.3,180 -47.6,180 -52.9,180 -58.2,180 -63.5,180 -68.8,180 -74.1,180 -79.4,180 -84.69999999999999,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -84.7,-180 -79.4,-180 -74.1,-180 -68.8,-180 -63.5,-180 -58.2,-180 -52.9,-180 -47.6,-180 -42.300000000000004,-180 -37))", "dataset_titles": "aBSREL tests for episodic diversifying selection on hemoglobin genes in notothenioids.; MEME tests of sites evolving under episodic diversifying selection in notothenioid hemoglobin genes.; Notothenioid hemoglobin protein 3D modeling.; Notothenioid species tree used in the study.; Phylogenetic trees of hemoglobin proteins in notothenioids.; Rates of hemoglobin evolution among genes and across notothenioid species.; RELAX tests for pervasive changes in strength of natural selection on hemoglobin genes in notothenioids.", "datasets": [{"dataset_uid": "601729", "doi": "10.15784/601729", "keywords": "Antarctica; Cold Adaptation; Cryonotothenioid; Dragonfish; Eleginopsioidea; Fish; Genetic Analysis; Hemoglobin; Icefish; Notothenioid; Notothenioid Fishes; Plunderfish; Sub-Antarctic", "people": "Postlethwait, John; Desvignes, Thomas", "repository": "USAP-DC", "science_program": null, "title": "Rates of hemoglobin evolution among genes and across notothenioid species.", "url": "https://www.usap-dc.org/view/dataset/601729"}, {"dataset_uid": "601728", "doi": "10.15784/601728", "keywords": "Antarctica; Cold Adaptation; Cryonotothenioid; Dragonfish; Eleginopsioidea; Fish; Gene; Genetic Analysis; Hemoglobin; Icefish; Notothenioid; Notothenioid Fishes; Plunderfish; Sub-Antarctic", "people": "Desvignes, Thomas; Postlethwait, John", "repository": "USAP-DC", "science_program": null, "title": "aBSREL tests for episodic diversifying selection on hemoglobin genes in notothenioids.", "url": "https://www.usap-dc.org/view/dataset/601728"}, {"dataset_uid": "601722", "doi": "10.15784/601722", "keywords": "Antarctica; Cold Adaptation; Cryonotothenioid; Dragonfish; Eleginopsioidea; Fish; Gene; Hemoglobin; Icefish; Notothenioid; Plunderfish; Sub-Antarctic", "people": "Postlethwait, John; Desvignes, Thomas", "repository": "USAP-DC", "science_program": null, "title": "Phylogenetic trees of hemoglobin proteins in notothenioids.", "url": "https://www.usap-dc.org/view/dataset/601722"}, {"dataset_uid": "601721", "doi": "10.15784/601721", "keywords": "Antarctica; Cold Adaptation; Cryonotothenioid; Dragonfish; Eleginopsioidea; Fish; Gene; Hemoglobin; Icefish; Notothenioid; Plunderfish; Sub-Antarctic", "people": "Desvignes, Thomas; Postlethwait, John", "repository": "USAP-DC", "science_program": null, "title": "Notothenioid species tree used in the study.", "url": "https://www.usap-dc.org/view/dataset/601721"}, {"dataset_uid": "601730", "doi": "10.15784/601730", "keywords": "Antarctica; Cold Adaptation; Cryonotothenioid; Dragonfish; Eleginopsioidea; Fish; Genetic Analysis; Hemoglobin; Icefish; Notothenioid; Notothenioid Fishes; Plunderfish; Sub-Antarctic", "people": "Desvignes, Thomas; Postlethwait, John", "repository": "USAP-DC", "science_program": null, "title": "MEME tests of sites evolving under episodic diversifying selection in notothenioid hemoglobin genes.", "url": "https://www.usap-dc.org/view/dataset/601730"}, {"dataset_uid": "601731", "doi": "10.15784/601731", "keywords": "Antarctica; Cold Adaptation; Cryonotothenioid; Dragonfish; Eleginopsioidea; Fish; Genetic Analysis; Hemoglobin; Icefish; Notothenioid; Notothenioid Fishes; Plunderfish; Sub-Antarctic", "people": "Desvignes, Thomas; Postlethwait, John", "repository": "USAP-DC", "science_program": null, "title": "RELAX tests for pervasive changes in strength of natural selection on hemoglobin genes in notothenioids.", "url": "https://www.usap-dc.org/view/dataset/601731"}, {"dataset_uid": "601732", "doi": "10.15784/601732", "keywords": "Antarctica; Cold Adaptation; Cryonotothenioid; Dragonfish; Eleginopsioidea; Fish; Genetic Analysis; Hemoglobin; Icefish; Notothenioid; Notothenioid Fishes; Plunderfish; Sub-Antarctic", "people": "Desvignes, Thomas; Postlethwait, John", "repository": "USAP-DC", "science_program": null, "title": "Notothenioid hemoglobin protein 3D modeling.", "url": "https://www.usap-dc.org/view/dataset/601732"}], "date_created": "Wed, 03 May 2023 00:00:00 GMT", "description": "Antarctic notothenioid fishes, also known as cryonotothenioids, inhabit the icy and highly oxygenated waters surrounding the Antarctic continent after diverging from notothenioids inhabiting more temperate waters. Notothenioid hemoglobin and blood parameters are known to have evolved along with the establishment of stable polar conditions, and among Antarctic notothenioids, icefishes are evolutionary oddities living without hemoglobin following the deletion of all functional hemoglobin genes from their genomes. In this project, we investigate the evolution of hemoglobin genes and gene clusters across the notothenioid radiation until their loss in the icefish ancestor after its divergence from the dragonfish lineage to understand the forces, mechanisms, and potential causes for hemoglobin gene loss in the icefish ancestor.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "FISH; Icefish; Cryonotothenioid; Gene; Plunderfish; Eleginopsioidea; AQUATIC ECOSYSTEMS; Dragonfish; Sub-Antarctic; Notothenioid; Blood; Hemoglobin", "locations": "Sub-Antarctic", "north": -37.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Desvignes, Thomas; Postlethwait, John", "platforms": null, "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -90.0, "title": "Evolution of hemoglobin genes in notothenioid fishes", "uid": "p0010417", "west": -180.0}, {"awards": "0231006 DeVries, Arthur; 1142158 Cheng, Chi-Hing", "bounds_geometry": "POLYGON((163 -76.5,163.5 -76.5,164 -76.5,164.5 -76.5,165 -76.5,165.5 -76.5,166 -76.5,166.5 -76.5,167 -76.5,167.5 -76.5,168 -76.5,168 -76.63,168 -76.76,168 -76.89,168 -77.02,168 -77.15,168 -77.28,168 -77.41,168 -77.54,168 -77.67,168 -77.8,167.5 -77.8,167 -77.8,166.5 -77.8,166 -77.8,165.5 -77.8,165 -77.8,164.5 -77.8,164 -77.8,163.5 -77.8,163 -77.8,163 -77.67,163 -77.54,163 -77.41,163 -77.28,163 -77.15,163 -77.02,163 -76.89,163 -76.76,163 -76.63,163 -76.5))", "dataset_titles": "High-resolution benthic seawater temperature record 1999-2012 (25-40m depth) from near intake jetty at McMurdo Station, Antarctica; Metadata associated with the description of Akarotaxis gouldae n. sp. (Bathydraconidae)", "datasets": [{"dataset_uid": "601811", "doi": null, "keywords": "Antarctica; Bellingshausen Sea; Cryosphere; Southern Ocean", "people": "McDowell, Jan; Steinberg, Deborah; Cheng, Chi-Hing; Biesack, Ellen; Corso, Andrew; Desvignes, Thomas; Hilton, Eric", "repository": "USAP-DC", "science_program": "LTER", "title": "Metadata associated with the description of Akarotaxis gouldae n. sp. (Bathydraconidae)", "url": "https://www.usap-dc.org/view/dataset/601811"}, {"dataset_uid": "601275", "doi": null, "keywords": "Antarctica; Benthic; McMurdo Sound; Mcmurdo Station; Oceans; Physical Oceanography; Temperature Probe; Water Temperature", "people": "Cziko, Paul; Cheng, Chi-Hing; Devries, Arthur", "repository": "USAP-DC", "science_program": null, "title": "High-resolution benthic seawater temperature record 1999-2012 (25-40m depth) from near intake jetty at McMurdo Station, Antarctica", "url": "https://www.usap-dc.org/view/dataset/601275"}], "date_created": "Wed, 08 Apr 2020 00:00:00 GMT", "description": "Antarctic notothenioid fishes exhibit two adaptive traits to survive in frigid temperatures. The first of these is the production of anti-freeze proteins in their blood and tissues. The second is a system-wide ability to perform cellular and physiological functions at extremely cold temperatures.The proposal goals are to show how Antarctic fishes use these characteristics to avoid freezing, and which additional genes are turned on, or suppressed in order for these fishes to maintain normal physiological function in extreme cold temperatures. Progressively colder habitats are encountered in the high latitude McMurdo Sound and Ross Shelf region, along with somewhat milder near?shore water environments in the Western Antarctic Peninsula (WAP). By quantifying the extent of ice crystals invading and lodging in the spleen, the percentage of McMurdo Sound fish during austral summer (Oct-Feb) will be compared to the WAP intertidal fish during austral winter (Jul-Sep) to demonstrate their capability and extent of freeze avoidance. Resistance to ice entry in surface epithelia (e.g. skin, gill and intestinal lining) is another expression of the adaptation of these fish to otherwise lethally freezing conditions. The adaptive nature of a uniquely characteristic polar genome will be explored by the study of the transcriptome (the set of expressed RNA transcripts that constitutes the precursor to set of proteins expressed by an entire genome). Three notothenioid species (E.maclovinus, D. Mawsoni and C. aceratus) will be analysed to document evolutionary genetic changes (both gain and loss) shaped by life under extreme chronic cold. A differential gene expression (DGE) study will be carried out on these different species to evaluate evolutionary modification of tissue-wide response to heat challenges. The transcriptomes and other sequencing libraries will contribute to de novo ice-fish genome sequencing efforts.", "east": 168.0, "geometry": "POINT(165.5 -77.15)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD", "is_usap_dc": true, "keywords": "McMurdo Sound; MARINE ECOSYSTEMS; Water Temperature; AQUATIC SCIENCES; OCEAN TEMPERATURE; FIELD INVESTIGATION; USAP-DC", "locations": "McMurdo Sound", "north": -76.5, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Cheng, Chi-Hing; Devries, Arthur", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -77.8, "title": "Antarctic Notothenioid Fish Freeze Avoidance and Genome-wide Evolution for Life in the Cold", "uid": "p0010091", "west": 163.0}, {"awards": "1440435 Ducklow, Hugh; 2023425 Schofield, Oscar", "bounds_geometry": "POLYGON((-80 -63,-78.3 -63,-76.6 -63,-74.9 -63,-73.2 -63,-71.5 -63,-69.8 -63,-68.1 -63,-66.4 -63,-64.7 -63,-63 -63,-63 -63.8,-63 -64.6,-63 -65.4,-63 -66.2,-63 -67,-63 -67.8,-63 -68.6,-63 -69.4,-63 -70.2,-63 -71,-64.7 -71,-66.4 -71,-68.1 -71,-69.8 -71,-71.5 -71,-73.2 -71,-74.9 -71,-76.6 -71,-78.3 -71,-80 -71,-80 -70.2,-80 -69.4,-80 -68.6,-80 -67.8,-80 -67,-80 -66.2,-80 -65.4,-80 -64.6,-80 -63.8,-80 -63))", "dataset_titles": "Environmental Data Initiative Repository, Supporting LTER; Expedition Data; Expedition data of LMG1501; Expedition data of LMG1601; Expedition data of LMG1701; Expedition data of LMG1801; Expedition data of LMG1901; Metadata associated with the description of Akarotaxis gouldae n. sp. (Bathydraconidae); UAV images and video of whales in the Antarctic Penisula during LMG1802", "datasets": [{"dataset_uid": "200125", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG1901", "url": "https://www.rvdata.us/search/cruise/LMG1901"}, {"dataset_uid": "002729", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG1701", "url": "https://www.rvdata.us/search/cruise/LMG1701"}, {"dataset_uid": "000246", "doi": "", "keywords": null, "people": null, "repository": "EDI", "science_program": null, "title": "Environmental Data Initiative Repository, Supporting LTER", "url": "https://portal.edirepository.org/nis/browseServlet?searchValue=PAL"}, {"dataset_uid": "601318", "doi": "10.15784/601318", "keywords": "Aerial Imagery; Antarctica; Antarctic Peninsula; Biota; Camera; Humpback Whales; LMG1802; LTER; Minke Whales; Oceans; Palmer Station; Photo; Photo/video; Photo/Video; R/v Laurence M. Gould; Species Size; UAV; Video Data; Whales", "people": "Bierlich, KC; Friedlaender, Ari; Dale, Julian; Nowacek, Douglas; Boyer, Keyvi", "repository": "USAP-DC", "science_program": "LTER", "title": "UAV images and video of whales in the Antarctic Penisula during LMG1802", "url": "https://www.usap-dc.org/view/dataset/601318"}, {"dataset_uid": "200124", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG1801", "url": "https://www.rvdata.us/search/cruise/LMG1801"}, {"dataset_uid": "001367", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG1701"}, {"dataset_uid": "200122", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG1501", "url": "https://www.rvdata.us/search/cruise/LMG1501"}, {"dataset_uid": "200123", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG1601", "url": "https://www.rvdata.us/search/cruise/LMG1601"}, {"dataset_uid": "601811", "doi": null, "keywords": "Antarctica; Bellingshausen Sea; Cryosphere; Southern Ocean", "people": "McDowell, Jan; Steinberg, Deborah; Cheng, Chi-Hing; Biesack, Ellen; Corso, Andrew; Desvignes, Thomas; Hilton, Eric", "repository": "USAP-DC", "science_program": "LTER", "title": "Metadata associated with the description of Akarotaxis gouldae n. sp. (Bathydraconidae)", "url": "https://www.usap-dc.org/view/dataset/601811"}], "date_created": "Fri, 11 May 2018 00:00:00 GMT", "description": "The Palmer Antarctica LTER (Long Term Ecological Research) site has been in operation since 1990. The goal of all the LTER sites is to conduct policy-relevant research on ecological questions that require tens of years of data, and cover large geographical areas. For the Palmer Antarctica LTER, the questions are centered around how the marine ecosystem west of the Antarctica peninsula is responding to a climate that is changing as rapidly as any place on the Earth. For example, satellite observations over the past 35 years indicate the average duration of sea ice cover is now ~90 days (3 months!) shorter than it was. The extended period of open water has implications for many aspects of ecosystem research, with the concurrent decrease of Ad\u00e8lie penguins within this region regularly cited as an exemplar of climate change impacts in Antarctica. Cutting edge technologies such as autonomous underwater (and possibly airborne) vehicles, seafloor moorings, and numerical modeling, coupled with annual oceanographic cruises, and weekly environmental sampling, enables the Palmer Antarctica LTER to expand and bridge the time and space scales needed to assess climatic impacts. This award includes for the first time study of the roles of whales as major predators in the seasonal sea ice zone ecosystem. The team will also focus on submarine canyons, special regions of enhanced biological activity, along the Western Antarctic Peninsula (WAP). The current award\u0027s overarching research question is: How do seasonality, interannual variability, and long term trends in sea ice extent and duration influence the structure and dynamics of marine ecosystems and biogeochemical cycling? Specific foci within the broad question include: 1. Long-term change and ecosystem transitions. What is the sensitivity or resilience of the ecosystem to external perturbations as a function of the ecosystem state? 2. Lateral connectivity and vertical stratification. What are the effects of lateral transports of freshwater, heat and nutrients on local ocean stratification and productivity and how do they drive changes in the ecosystem? 3. Top-down controls and shifting baselines. How is the ecosystem responding to the cessation of whaling and subsequent long-term recovery of whale stocks? 4. Foodweb structure and biogeochemical processes. How do temporal and spatial variations in foodweb structure influence carbon and nutrient cycling, export, and storage? The broader impacts of the award leverage local educational partnerships including the Sandwich, MA STEM Academy, the New England Aquarium, and the NSF funded Polar Learning and Responding (PoLAR) Climate Change Education Partnership at Columbia\u0027s Earth Institute to build new synergies between Arctic and Antarctic, marine and terrestrial scientists and students, governments and NGOs. The Palmer Antarctic LTER will also conduct appropriate cross LTER site comparisons, and serve as a leader in information management to enable knowledge-building within and beyond the Antarctic, oceanographic, and LTER communities.", "east": -63.0, "geometry": "POINT(-71.5 -67)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; IN SITU/LABORATORY INSTRUMENTS \u003e CHEMICAL METERS/ANALYZERS \u003e FLUOROMETERS; IN SITU/LABORATORY INSTRUMENTS \u003e RECORDERS/LOGGERS \u003e AWS; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; EARTH REMOTE SENSING INSTRUMENTS \u003e ACTIVE REMOTE SENSING \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ECHO SOUNDERS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e THERMOSALINOGRAPHS", "is_usap_dc": true, "keywords": "PELAGIC; USAP-DC; R/V LMG; NOT APPLICABLE; Palmer Station; LMG1701", "locations": "Palmer Station", "north": -63.0, "nsf_funding_programs": "Antarctic Integrated System Science; Antarctic Organisms and Ecosystems; Antarctic Integrated System Science; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Ducklow, Hugh; Martinson, Doug; Schofield, Oscar", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE; WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "R2R", "repositories": "EDI; R2R; USAP-DC", "science_programs": "LTER", "south": -71.0, "title": "LTER Palmer, Antarctica (PAL): Land-Shelf-Ocean Connectivity, Ecosystem Resilience and Transformation in a Sea-Ice Influenced Pelagic Ecosystem", "uid": "p0000133", "west": -80.0}, {"awards": "1142122 Miller, Nathan", "bounds_geometry": "POLYGON((166.163 -76.665,166.2635 -76.665,166.364 -76.665,166.4645 -76.665,166.565 -76.665,166.6655 -76.665,166.766 -76.665,166.8665 -76.665,166.967 -76.665,167.0675 -76.665,167.168 -76.665,167.168 -76.782,167.168 -76.899,167.168 -77.016,167.168 -77.133,167.168 -77.25,167.168 -77.367,167.168 -77.484,167.168 -77.601,167.168 -77.718,167.168 -77.835,167.0675 -77.835,166.967 -77.835,166.8665 -77.835,166.766 -77.835,166.6655 -77.835,166.565 -77.835,166.4645 -77.835,166.364 -77.835,166.2635 -77.835,166.163 -77.835,166.163 -77.718,166.163 -77.601,166.163 -77.484,166.163 -77.367,166.163 -77.25,166.163 -77.133,166.163 -77.016,166.163 -76.899,166.163 -76.782,166.163 -76.665))", "dataset_titles": "Antarctic emerald rockcod have the capacity to compensate for warming when uncoupled from CO2-acidification; Physiological and biochemical measurements on Antarctic dragonfish (Gymnodraco acuticeps) from McMurdo Sound; Physiological and biochemical measurements on juvenile Antarctic rockcod (Trematomus bernacchii) from McMurdo Sound; Thermal windows and metabolic performance curves in a developing Antarctic fish", "datasets": [{"dataset_uid": "601039", "doi": "10.15784/601039", "keywords": "Antarctica; Biota; Chemistry:fluid; Chemistry:Fluid; CTD Data; Fish; McMurdo Sound; Ocean Acidification; Oceans; Physical Oceanography; Ross Sea; Southern Ocean", "people": "Todgham, Anne; Miller, Nathan", "repository": "USAP-DC", "science_program": null, "title": "Antarctic emerald rockcod have the capacity to compensate for warming when uncoupled from CO2-acidification", "url": "https://www.usap-dc.org/view/dataset/601039"}, {"dataset_uid": "601026", "doi": "10.15784/601026", "keywords": "Antarctica; Biota; Chemistry:fluid; Chemistry:Fluid; CTD Data; Fish; McMurdo Sound; Ocean Acidification; Oceans; Physical Oceanography; Ross Sea; Southern Ocean", "people": "Davis, Brittany; Miller, Nathan; Todgham, Anne; Flynn, Erin", "repository": "USAP-DC", "science_program": null, "title": "Physiological and biochemical measurements on Antarctic dragonfish (Gymnodraco acuticeps) from McMurdo Sound", "url": "https://www.usap-dc.org/view/dataset/601026"}, {"dataset_uid": "601025", "doi": "10.15784/601025", "keywords": "Antarctica; Biota; Fish; McMurdo Sound; Oceans; Ross Sea; Southern Ocean", "people": "Todgham, Anne; Davis, Brittany; Flynn, Erin; Miller, Nathan", "repository": "USAP-DC", "science_program": null, "title": "Physiological and biochemical measurements on juvenile Antarctic rockcod (Trematomus bernacchii) from McMurdo Sound", "url": "https://www.usap-dc.org/view/dataset/601025"}, {"dataset_uid": "601040", "doi": "10.15784/601040", "keywords": "Antarctica; Biota; Fish; McMurdo Sound; Oceans; Ross Sea; Sample/collection Description; Sample/Collection Description; Southern Ocean", "people": "Todgham, Anne; Miller, Nathan", "repository": "USAP-DC", "science_program": null, "title": "Thermal windows and metabolic performance curves in a developing Antarctic fish", "url": "https://www.usap-dc.org/view/dataset/601040"}], "date_created": "Tue, 15 Aug 2017 00:00:00 GMT", "description": "Ocean acidification and increased temperatures are projected to be the primary impacts of global climate change on polar marine ecosystems over the next century. While recent research has focused on the effects of these drivers on calcifying organisms, less is known about how these changes may affect vertebrates. This research will focus on two Antarctic fishes, Trematomus bernacchii and Pagothenia borchgrevinki. Fish eggs and larvae will be collected in McMurdo Sound and reared under different temperature and pH regimes. Modern techniques will be used to examine subsequent changes in physiology, growth, development and gene expression over both short and long timescales. The results will fill a missing gap in our knowledge about the response of non-calcifying organisms to projected changes in pH and temperature. Results will be widely disseminated through publications as well as through presentations at national and international meetings; raw data will also be made available through open-access, web-based databases. This project will support the research and training of three graduate and three undergraduate students. As well, this project will foster the development of two modules on climate change and ocean acidification for an Introduction to Biology course.", "east": 167.168, "geometry": "POINT(166.6655 -77.25)", "instruments": null, "is_usap_dc": true, "keywords": "Not provided", "locations": null, "north": -76.665, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Miller, Nathan; Todgham, Anne", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -77.835, "title": "RUI: Synergistic effects of Ocean Acidification and Warming on Larval Development in Antarctic Fishes", "uid": "p0000411", "west": 166.163}, {"awards": "0636696 DeVries, Arthur", "bounds_geometry": "POLYGON((-68.0025 -52.7599,-67.07254 -52.7599,-66.14258 -52.7599,-65.21262 -52.7599,-64.28266 -52.7599,-63.3527 -52.7599,-62.42274 -52.7599,-61.49278 -52.7599,-60.56282 -52.7599,-59.63286 -52.7599,-58.7029 -52.7599,-58.7029 -53.98242,-58.7029 -55.20494,-58.7029 -56.42746,-58.7029 -57.64998,-58.7029 -58.8725,-58.7029 -60.09502,-58.7029 -61.31754,-58.7029 -62.54006,-58.7029 -63.76258,-58.7029 -64.9851,-59.63286 -64.9851,-60.56282 -64.9851,-61.49278 -64.9851,-62.42274 -64.9851,-63.3527 -64.9851,-64.28266 -64.9851,-65.21262 -64.9851,-66.14258 -64.9851,-67.07254 -64.9851,-68.0025 -64.9851,-68.0025 -63.76258,-68.0025 -62.54006,-68.0025 -61.31754,-68.0025 -60.09502,-68.0025 -58.8725,-68.0025 -57.64998,-68.0025 -56.42746,-68.0025 -55.20494,-68.0025 -53.98242,-68.0025 -52.7599))", "dataset_titles": "Expedition Data; Expedition data of LMG0809; Metadata associated with the description of Akarotaxis gouldae n. sp. (Bathydraconidae)", "datasets": [{"dataset_uid": "601811", "doi": null, "keywords": "Antarctica; Bellingshausen Sea; Cryosphere; Southern Ocean", "people": "McDowell, Jan; Steinberg, Deborah; Cheng, Chi-Hing; Biesack, Ellen; Corso, Andrew; Desvignes, Thomas; Hilton, Eric", "repository": "USAP-DC", "science_program": "LTER", "title": "Metadata associated with the description of Akarotaxis gouldae n. sp. (Bathydraconidae)", "url": "https://www.usap-dc.org/view/dataset/601811"}, {"dataset_uid": "001493", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0810"}, {"dataset_uid": "002728", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0809", "url": "https://www.rvdata.us/search/cruise/LMG0809"}, {"dataset_uid": "001504", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0809"}], "date_created": "Tue, 04 May 2010 00:00:00 GMT", "description": "Antarctic notothenioid fish evolved antifreeze (AF) proteins that prevent ice crystals that enter their body fluids from growing, and thereby avoid freezing in their icy habitats. However, even in the extreme cold Antarctic marine environment, regional gradations of severity are found. The biological correlate for environmental severity in fish is the endogenous ice load, which likely determines the tolerable limit of environmental severity for notothenioid habitation. The endogenous ice load develops from environmental ice crystals entering through body surfaces and somehow localizing to the spleen. How prone the surface tissues are to ice entry, how ice reaches the spleen, and what the fate of splenic ice is, requires elucidation. Spleen sequestration of ice raises the hypothesis that macrophages may play a role in the translocation and perhaps elimination of AF-bound ice crystals. Antifreeze glycoproteins (AFGP) act in concert with a second, recently discovered antifreeze called antifreeze potentiating protein (AFPP), necessitating an assessment of the contribution of AFPP to freezing avoidance. Recent research suggests that the exocrine pancreas and the anterior stomach, not the liver, synthesize AFGPs and secrete them into the intestine, from where they may be returned to the blood. A GI-to-blood transport is a highly unconventional path for a major plasma protein and also begs the questions, What is the source of blood AFPP?. Why are two distinct AF proteins needed and what is the chronology of their evolution? What genomic changes in the DNA are associated with the development or loss of the antifreeze trait? Experiments described in this proposal address these interrelated questions of environmental, organismal, and evolutionary physiology, and will further our understanding of novel vertebrate physiologies, the limits of environmental adaptation, and climatically driven changes in the genome. The proposed research will (1) determine the temporal and spatial heterogeneity of environmental temperature and iciness in progressively more severe fish habitats in the greater McMurdo Sound area, and in the milder Arthur Harbor at Palmer Station. The splenic ice load in fishes inhabiting these sites will be determined to correlate to environmental severity and habitability. (2) Assess the surface tissue site of ice entry and their relative barrier properties in intact fish and isolated tissues preparations (3) Assess the role of immune cells in the fate of endogenous ice, (4) determine whether the blood AFGPs are from intestinal/rectal uptake, (5) examine the contribution of AFPP to the total blood AF activity (6) evaluate the progression of genomic changes in the AFGP locus across Notothenioidei as modulated by disparate thermal environments, in four selected species through the analyses of large insert DNA BAC clones. The origin and evolution of AFPP will be examined also by analyzing BAC clones encompassing the AFPP genomic locus. The broader impacts of the proposed research include training of graduate and undergraduate students in research approaches ranging from physical field measurements to cutting edge genomics. Undergraduate research projects have lead to co-authored publications and will continue to do so. Outreach includes establishing Wiki websites on topics of Antarctic fish biology and freeze avoidance, providing advisory services to the San Francisco Science Exploratorium, and making BAC libraries available to interested polar biologists. This research theme has repeatedly received national and international science news coverage and will continue to be disseminated to the public in that manner.", "east": -58.7029, "geometry": "POINT(-63.3527 -58.8725)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e CTD; EARTH REMOTE SENSING INSTRUMENTS \u003e PASSIVE REMOTE SENSING \u003e POSITIONING/NAVIGATION \u003e GPS \u003e GPS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e ADCP", "is_usap_dc": false, "keywords": "R/V LMG", "locations": null, "north": -52.7599, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Devries, Arthur", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG", "repo": "USAP-DC", "repositories": "R2R; USAP-DC", "science_programs": null, "south": -64.9851, "title": "Environmental, Organismal and Evolutionary Physiology of Freeze Avoidance in Antarctic Notothenioid Fishes", "uid": "p0000560", "west": -68.0025}]

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