USAP-DC

{"dp_type": "Project", "free_text": "BACTERIA/ARCHAEA"}

[{"awards": "2142914 Baker, Bill; 2142913 Tresguerres, Martin; 2142912 Murray, Alison", "bounds_geometry": "POLYGON((-180 -60,-168 -60,-156 -60,-144 -60,-132 -60,-120 -60,-108 -60,-96 -60,-84 -60,-72 -60,-60 -60,-60 -62,-60 -64,-60 -66,-60 -68,-60 -70,-60 -72,-60 -74,-60 -76,-60 -78,-60 -80,-72 -80,-84 -80,-96 -80,-108 -80,-120 -80,-132 -80,-144 -80,-156 -80,-168 -80,180 -80,178 -80,176 -80,174 -80,172 -80,170 -80,168 -80,166 -80,164 -80,162 -80,160 -80,160 -78,160 -76,160 -74,160 -72,160 -70,160 -68,160 -66,160 -64,160 -62,160 -60,162 -60,164 -60,166 -60,168 -60,170 -60,172 -60,174 -60,176 -60,178 -60,-180 -60))", "dataset_titles": null, "datasets": null, "date_created": "Thu, 17 Oct 2024 00:00:00 GMT", "description": "Non-technical description Marine invertebrates often have mutually beneficial partnerships with microorganisms that biosynthesize compounds with nutritive or defensive functions and are integral for survival. Additionally, these \u201cnatural products\u201d often have bioactive properties with human health applications fighting infection or different types of cancer. This project focuses on the ascidian (\u201csea squirt\u201d) Synoicum adareanum, found in the Anvers Island region of the Antarctic Peninsula, and was recently discovered to contain high levels of a natural product, palmerolide A (palA) in its tissues. The microorganism that produces palA is a new bacterial species, Candidatus Synoicihabitans palmerolidicus, found in a persistent partnership with the sea squirt. There is still much to be learned about the fundamental properties of this sea squirt-microbe-palA system including the geographical range of the animal-microbe partnership, its chemical and microbiome complexity and diversity, and the biological effect of palA in the sea squirt. To address these questions, this multidisciplinary research team will investigate the sea squirt-microbiome partnership in the Antarctic Peninsula and McMurdo Sound regions of the Ross Sea using a state-of-the-art strategy that will advance our understanding of the structural and functional features of the sea squirt and microbiome in detail, and reveal the roles that the palA natural product plays in the host ecology in its native Antarctic seafloor habitat. The project will broaden diversity and provide new opportunities for early career students and postdoctoral researchers to participate in field and laboratory-based research that builds an integrative understanding of Antarctic marine biology, ecology, physiology and chemistry. In addition, advancing the understanding of palA and its biological properties may be of future benefit to biomedicine and human health. Technical description Marine invertebrates and their associated microbiomes can produce bioactive natural products; in fact, \u003e600 such compounds have been identified in species from polar waters. Although such compounds are typically hypothesized to serve ecological roles in host survival through deterring predation, fouling, and microbial infection, in most cases neither the producing organism nor the genome-encoded biosynthetic enzymes are known. This project will study an emerging biosynthetic system from a polar ascidian-microbe association that produces palA, a natural product with bioactivity against the proton-pumping enzyme V-type H+-ATPase (VHA). The objectives include: (i) Determining the microbiome composition, metabolome complexity, palA levels, and mitochondrial DNA sequence of S. adareanum morphotypes at sites in the Antarctic Peninsula and in McMurdo Sound, (ii) Characterizing the Synoicum microbiome using a multi-omics strategy, and (iii) Assessing the potential for co-occurrence of Ca. S. palmerolidicus-palA-VHA in host tissues, and (iv) exploring the role of palA in modulating VHA activity in vivo and its effects on ascidian-microbe ecophysiology. Through a coupled study of palA-producing and non-producing S. adareanum specimens, structural and functional features of the ascidian microbiome metagenome will be characterized to better understand the relationship between predicted secondary metabolite pathways and whether they are expressed in situ using a paired metatranscriptome sequencing and secondary metabolite detection strategy. Combined with tissue co-localization results, functional ecophysiological assays aim to determine the roles that the natural product plays in the host ecology in its native Antarctic seafloor habitat. The contributions of the project will inform this intimate host-microbial association in which the ascidian host bioaccumulates VHA-inhibiting palA, yet its geo-spatial distribution, cellular localization, ecological and physiological role(s) are not known. In addition to elucidating the ecophysiological roles of palA in their native ascidian-microbe association, the results will contribute to the success of translational science, which aligns with NSF\u2019s interests in promoting basic research that leads to advances in Biotechnology and Bioeconomy. The project will also broaden diversity and provide new opportunities for early career students and postdoctoral researchers to participate in field and laboratory-based research that builds an integrative understanding of Antarctic marine biology, ecology, physiology and chemistry. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 160.0, "geometry": "POINT(-130 -70)", "instruments": null, "is_usap_dc": true, "keywords": "ECOSYSTEM FUNCTIONS; BACTERIA/ARCHAEA; BENTHIC; R/V NBP; Antarctic Peninsula; ANIMALS/INVERTEBRATES", "locations": "Antarctic Peninsula", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Baker, Bill; Murray, Alison; Tresguerres, Martin", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repositories": null, "science_programs": null, "south": -80.0, "title": "Collaborative Research: ANT LIA: Diving into the Ecology of an Antarctic Ascidian-Microbiome-Palmerolide Association using a Multi-omic and Functional Approach", "uid": "p0010485", "west": -60.0}, {"awards": "2031121 Junge, Karen", "bounds_geometry": null, "dataset_titles": null, "datasets": null, "date_created": "Tue, 30 Jul 2024 00:00:00 GMT", "description": "The ozone hole that develops over the Antarctic continent every spring is one of the features attributed to human activity, in particular production of the CFC (chlorofluorocarbons in refrigerants) released to the atmosphere. In spite of the CFC ban from the Montreal Protocol established in the year 1987, the recovery has been slower than predicted. Bromocarbons, known to produce the stratospheric ozone depletion, have recently been estimated to contribute to the pool of bromines in the lower atmosphere. What is the origin of the bromocarbons in Antarctic sea ice? Is this an additional source of chemicals creating the ozone hole? This project will test if bromocarbons in sea ice are produced and degraded by microalgae and bacteria found in sea ice, in snow and the interface between the two. The project will collect a suite of chemical and biological measurements of sea ice and snow to determine bromocarbon concentrations, microbial activity associated with them, and intra-cellular genes and proteins involved in bromocarbon metabolism. This project benefits NSF\u2019s goals of expanding fundamental knowledge of Antarctic systems, biota, and processes, and improving the understanding of interactions among the Antarctic systems, cryosphere and organisms. The work will be carried out at McMurdo Station in late austral spring, including sampling of snow and ice that will be concentrated in the laboratory, and 24-hour experiments to measure algal and bacterial activity. Genes controlling synthesis of enzymes involved in bromocarbon metabolism are of interest in biotechnology and bioremediation, including products that repair damaged skin from UltraViolet Radiation. The project will train undergraduate students on chemical and biological techniques. The Principal Investigators will be involved in the Pacific Science Center in Seattle with ~10,000 visitors per weekend where they will develop a project-specific exhibit. The microbial processes in snow and ice associated with bromocarbon synthesis and degradation have not been studied in Antarctica during winter and spring. This study will inform about microbial activity in relation to the release of bromocarbons compounds from the snow and ice surfaces, compounds known to degrade stratospheric ozone. The estimation of chemical bromocarbons will be combined with metagenomics and metaproteomics approaches for understanding the potential role of microbes in snow and sea ice. The environmental, chemical and biological data will be synthesized with multivariate analysis and significant differences between sites and experimental treatments with ANOVA. A collaborator from the University of Goteborg in Sweden will collaborate on bromocarbon analyses. The study will also address \u201csaline snow\u201d a new environment not previously studied for microbial life. In addition, this is the first study of meta-proteomics in snow and ice. The Principal Investigators expect their results will help inform ozone hole recovery in the 21st century. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "McMurdo Sound; COASTAL; BACTERIA/ARCHAEA; SEA ICE; SNOW/ICE", "locations": "McMurdo Sound", "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Junge, Karen; Nunn, Brook L", "platforms": null, "repositories": null, "science_programs": null, "south": null, "title": "Sea-ice Snow Microbial Communities\u2019 Impact on Antarctic Bromocarbon Budgets and Processes", "uid": "p0010472", "west": null}, {"awards": "2224760 Gooseff, Michael", "bounds_geometry": "POINT(162.87 -77)", "dataset_titles": "EDI Data Portal: McMurdo Dry Valleys LTER", "datasets": [{"dataset_uid": "200379", "doi": "", "keywords": null, "people": null, "repository": "Environmental Data Initiative (EDI)", "science_program": null, "title": "EDI Data Portal: McMurdo Dry Valleys LTER", "url": "https://portal.edirepository.org/nis/browseServlet?searchValue=MCM"}], "date_created": "Tue, 14 Nov 2023 00:00:00 GMT", "description": "Non-technical Abstract The McMurdo Dry Valleys LTER seeks to understand how changes in the temporal variability of ecological connectivity interact with existing landscape legacies to alter the structure and functioning of this extreme polar desert ecosystem. This research has broad implications, as it will help us to understand how natural ecosystems respond to ongoing anthropogenic global change. At the same time, this project also serves an important educational and outreach function, providing immersive research and educational experiences to students and artists from diverse backgrounds, and helping to ensure a diverse and well-trained next generation of leaders in polar ecosystem science and stewardship. Ultimately, the results of this project will help us to better understand and prepare for the effects of climate change and develop scientific insights that are relevant far beyond Antarctic ecosystems. The McMurdo Dry Valleys (MDVs) make up an extreme polar desert ecosystem in the largest ice-free region of Antarctica. The organisms in this ecosystem are generally small. Bacteria, microinvertebrates, cyanobacterial mats, and phytoplankton can be found across the streams, soils, glaciers, and ice-covered lakes. These organisms have adapted to the cold and arid conditions that prevail outside of lakes for all but a brief period in the austral summer when the ecosystem is connected by liquid water. In the summer when air temperatures rise barely above freezing, soils warm and glacial meltwater flows through streams into the open moats of lakes. Most biological activity across the landscape occurs in summer. Through the winter, or polar night (6 months of darkness), glaciers, streams, and soil biota are inactive until sufficient light, heat, and liquid water return, while lake communities remain active all year. Over the past 30 years, the MDVs have been disturbed by cooling, heatwaves, floods, rising lake levels, as well as permafrost and lake ice thaw. Considering the clear ecological responses to this variation in physical drivers, and climate models predicting further warming and more precipitation, the MDV ecosystem sits at a threshold between the current extreme cold and dry conditions and an uncertain future. This project seeks to determine how important the legacy of past events and conditions versus current physical and biological interactions shape the current ecosystem. Four hypotheses will be tested, related to 1) whether the status of specific organisms are indicative ecosystem stability, 2) the relationship between legacies of past events to current ecosystem resilience (resistance to big changes), 3) carryover of materials between times of high ecosystem connectivity and activity help to maintain ecosystem stability, and 4) changes in disturbances affect how this ecosystem persists through the annual polar night (i.e., extended period of dark and cold). Technical Abstract In this iteration of the McMurdo LTER project (MCM6), the project team will test ecological connectivity and stability theory in a system subject to strong physical drivers (geological legacies, extreme seasonality, and contemporary climate change) and driven by microbial organisms. Since microorganisms regulate most of the world\u2019s critical biogeochemical functions, these insights will be relevant far beyond polar ecosystems and will inform understanding and expectations of how natural and managed ecosystems respond to ongoing anthropogenic global change. MCM6 builds on previous foundational research, both in Antarctica and within the LTER network, to consider the temporal aspects of connectivity and how it relates to ecosystem stability. The project will examine how changes in the temporal variability of ecological connectivity interact with the legacies of the existing landscape that have defined habitats and biogeochemical cycling for millennia. The project team hypothesizes that the structure and functioning of the MDV ecosystem is dependent upon legacies and the contemporary frequency, duration, and magnitude of ecological connectivity. This hypothesis will be tested with new and continuing monitoring, experiments, and analyses of long-term datasets to examine: 1) the stability of these ecosystems as reflected by sentinel taxa, 2) the relationship between ecological legacies and ecosystem resilience, 3) the importance of material carryover during periods of low connectivity to maintaining biological activity and community stability, and 4) how changes in disturbance dynamics disrupt ecological cycles through the polar night. Tests of these hypotheses will occur in field and modeling activities using new and long-term datasets already collected. New datasets resulting from field activities will be made freely available via widely-known online databases (MCM LTER and EDI). The project team has also developed six Antarctic Core Ideas that encompass themes from data literacy to polar food webs and form a consistent thread across the education and outreach activities. Building on past success, collaborations will be established with teachers and artists embedded within the science teams, who will work to develop educational modules with science content informed by direct experience and artistic expression. Undergraduate mentoring efforts will incorporate computational methods through a new data-intensive scientific training program for MCM REU students. The project will also establish an Antarctic Research Experience for Community College Students at CU Boulder, to provide an immersive educational and research experience for students from diverse backgrounds in community colleges. MCM LTER will continue its mission of training and mentoring students, postdocs, and early career scientists as the next generation of leaders in polar ecosystem science and stewardship. Historically underrepresented participation will be expanded at each level of the project. To aid in these efforts, the project has established Education \u0026 Outreach and Diversity, Equity, and Inclusion committees to lead, coordinate, support, and integrate these activities through all aspects of MCM6. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 162.87, "geometry": "POINT(162.87 -77)", "instruments": null, "is_usap_dc": true, "keywords": "COMMUNITY DYNAMICS; ABLATION ZONES/ACCUMULATION ZONES; SOIL TEMPERATURE; DIATOMS; FIELD INVESTIGATION; PERMANENT LAND SITES; BUOYS; GROUND-BASED OBSERVATIONS; SEDIMENTS; SNOW WATER EQUIVALENT; SPECIES/POPULATION INTERACTIONS; WATER-BASED PLATFORMS; FIXED OBSERVATION STATIONS; VIRUSES; PHYTOPLANKTON; ACTIVE LAYER; FIELD SURVEYS; RADIO TRANSMITTERS; DATA COLLECTIONS; ECOLOGICAL DYNAMICS; LANDSCAPE; GROUND WATER; SNOW/ICE CHEMISTRY; LAND-BASED PLATFORMS; ANIMALS/INVERTEBRATES; ECOSYSTEM FUNCTIONS; HUMIDITY; GEOCHEMISTRY; SURFACE WINDS; RIVERS/STREAM; GLACIER MASS BALANCE/ICE SHEET MASS BALANCE; SNOW; LAND RECORDS; ATMOSPHERIC PRESSURE; SURFACE TEMPERATURE; ATMOSPHERIC RADIATION; BACTERIA/ARCHAEA; AIR TEMPERATURE; GLACIERS; SNOW/ICE TEMPERATURE; SOIL CHEMISTRY; METEOROLOGICAL STATIONS; WATER QUALITY/WATER CHEMISTRY; TERRESTRIAL ECOSYSTEMS; MOORED; PROTISTS; STREAMFLOW STATION; Dry Valleys; LAKE/POND; LAKE ICE; SNOW DEPTH; AQUATIC ECOSYSTEMS; SNOW DENSITY; FIELD SITES", "locations": "Dry Valleys", "north": -77.0, "nsf_funding_programs": "Antarctic Integrated System Science; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Gooseff, Michael N.; Adams, Byron; Barrett, John; Diaz, Melisa A.; Doran, Peter; Dugan, Hilary A.; Mackey, Tyler; Morgan-Kiss, Rachael; Salvatore, Mark; Takacs-Vesbach, Cristina; Zeglin, Lydia H.", "platforms": "LAND-BASED PLATFORMS; LAND-BASED PLATFORMS \u003e FIELD SITES; LAND-BASED PLATFORMS \u003e FIELD SITES \u003e DATA COLLECTIONS; LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION; LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS; LAND-BASED PLATFORMS \u003e FIELD SITES \u003e RADIO TRANSMITTERS; LAND-BASED PLATFORMS \u003e PERMANENT LAND SITES; LAND-BASED PLATFORMS \u003e PERMANENT LAND SITES \u003e FIXED OBSERVATION STATIONS; LAND-BASED PLATFORMS \u003e PERMANENT LAND SITES \u003e GROUND-BASED OBSERVATIONS; LAND-BASED PLATFORMS \u003e PERMANENT LAND SITES \u003e METEOROLOGICAL STATIONS; LAND-BASED PLATFORMS \u003e PERMANENT LAND SITES \u003e STREAMFLOW STATION; WATER-BASED PLATFORMS; WATER-BASED PLATFORMS \u003e BUOYS; WATER-BASED PLATFORMS \u003e BUOYS \u003e MOORED; WATER-BASED PLATFORMS \u003e BUOYS \u003e MOORED \u003e BUOYS", "repo": "Environmental Data Initiative (EDI)", "repositories": "Environmental Data Initiative (EDI)", "science_programs": "LTER", "south": -77.0, "title": "LTER: MCM6 - The Roles of Legacy and Ecological Connectivity in a Polar Desert Ecosystem", "uid": "p0010440", "west": 162.87}, {"awards": "2228257 Michaud, Alexander", "bounds_geometry": "POINT(-112.05 -79.28)", "dataset_titles": null, "datasets": null, "date_created": "Wed, 31 May 2023 00:00:00 GMT", "description": "Ice cores from glaciers and ice sheets provide detailed archives of past environmental conditions, furthering our understanding of Earth\u2019s climate. Microorganisms in the West Antarctic Ice Sheet are buried over glaciological time and form a stratigraphy record providing the opportunity of analysis of the order and position of layers of geological events, with potential links to Southern Hemisphere climate. However, microbial cells that land on the ice sheet are subject to the stresses of changing habitat conditions due to burial and conditions associated with long-term isolation in ice. These processes may lead to a loss of fidelity within the stratigraphic record of microbial cells. We know little about how and if microorganisms survive burial and remain alive over glacial-interglacial time periods within an ice sheet. This analysis will identify the viable and preserved community of microorganisms and core genomic adaptation that permit cell viability, which will advance knowledge in the areas of microbiology and glaciology while increasing fidelity of ice core measurements relevant to past climate and potential future global climate impacts. This exploratory endeavor has the potential to be a transformative step toward understanding the ecology of one of the most understudied environments on Earth. The project will partner with the Museum of Science, Boston, to increase public scientific literacy via education and outreach. Additionally, this project will support two early-career scientists and two undergraduates in interdisciplinary research at the intersection of microbiology and climate science. Results from this project will provide the first DNA data based on single-cell whole genomic sequencing from the Antarctic Ice Sheet and inform whether post-depositional processes impact the interpretations of paleoenvironmental conditions from microbes. The goals to determine the taxonomic identity of viable and preserved microbial cells, and decode the genetic repertoire that confers survival of burial and long-term viability within glacial ice, will be achieved by utilizing subsamples from a ~60,000 year old record of the West Antarctic Ice Sheet Divide (WD) Ice Core. WD samples will be melted using the Desert Research Institute\u2019s ice core melting system that is optimized for glaciobiological sampling. Microbial cells from the meltwater will be sorted using fluorescence-activated cell sorting, and individually sorted cells will have their genomes sequenced. The fluorescence-based methods will discern the viable (metabolically active) cells from those cells that are non-viable but preserved in the ice (DNA-containing). The genomic analysis will identify the taxonomy of each cell, presence of known genes that confer survival in permanently frozen environments, and comparatively analyze genomes to determine the core set of genes required by viable cells to persist in an ice sheet. The outcomes of this work will expand the potential for biological measurements and contamination control from archived ice cores. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": -112.05, "geometry": "POINT(-112.05 -79.28)", "instruments": null, "is_usap_dc": true, "keywords": "WAIS Divide; TERRESTRIAL ECOSYSTEMS; ICE SHEETS; BACTERIA/ARCHAEA; ICE CORE RECORDS", "locations": "WAIS Divide", "north": -79.28, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Michaud, Alexander; Winski, Dominic A.", "platforms": null, "repositories": null, "science_programs": null, "south": -79.28, "title": "EAGER: ANT LIA: Persist or Perish: Records of Microbial Survival and Long-term Persistence from the West Antarctic Ice Sheet", "uid": "p0010421", "west": -112.05}, {"awards": "2133685 Adams, Byron; 2133684 Fierer, Noah", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "Geochemical, physicochemical, and genomic data from a continental-scale survey of microbial diversity in Antarctic soils", "datasets": [{"dataset_uid": "200478", "doi": "10.6073/pasta/b4858653c587864f0111aba4c3014d61", "keywords": null, "people": null, "repository": "EDI", "science_program": null, "title": "Geochemical, physicochemical, and genomic data from a continental-scale survey of microbial diversity in Antarctic soils", "url": "https://portal.edirepository.org/nis/mapbrowse?packageid=knb-lter-mcm.275.1"}], "date_created": "Fri, 07 Apr 2023 00:00:00 GMT", "description": "Not all of Antarctica is covered in ice. In fact, soils are common to many parts of Antarctica, and these soils are often unlike any others found on Earth. Antarctic soils harbor unique microorganisms able to cope with the extremely cold and dry conditions common to much of the continent. For decades, microbiologists have been drawn to the unique soils in Antarctica, yet critical knowledge gaps remain. Most notably, it is unclear what properties allow certain microbes to thrive in Antarctic soils. By using a range of methods, this project is developing comprehensive model that discovers the unique genomic features of soils diversity, distributions, and adaptations that allow Antarctic soil microbes to thrive in extreme environments. The proposed work will be relevant to researchers in many fields, including engineers seeking to develop new biotechnologies, ecologists studying the contributions of these microbial communities to the functioning of Antarctic ecosystems, microbiologists studying novel microbial adaptations to extreme environmental conditions, and even astrobiologists studying the potential for life on Mars. More generally, the proposed research presents an opportunity to advance our current understanding of the microbial life found in one of the more distinctive microbial habitats on Earth, a habitat that is inaccessible to many scientists and a habitat that is increasingly under threat from climate change. The research project explores the microbial diversity in Antarctic soils and links specific features to different soil types and environmental conditions. The overarching questions include: What microbial taxa are found in a variety of Antarctic environments? What are the environmental preferences of specific taxa or lineages? What are the genomic and phenotypic traits of microorganisms that allow them to persist in extreme environments and determine biogeographical differneces? This project will analyze archived soils collected from across Antarctica by a network of international collaborators, with samples selected to span broad gradients in soil and site conditions. The project uses cultivation-independent, high-throughput genomic analysis methods and cultivation-dependent approaches to analyze bacterial and fungal communities in soil samples. The results will be used to predict the distributions of specific taxa and lineages, obtain genomic information for the more ubiquitous and abundant taxa, and quantify growth responses in vitro across gradients in temperature, moisture, and salinity. This integration of ecological, environmental, genomic, and trait-based information will provide a comprehensive understanding of microbial life in Antarctic soils. This project will also help facilitate new collaborations between scientists across the globe while providing undergraduate students with \u0027hands-on\u0027 research experiences that introduce the next generation of scientists to the field of Antarctic biology. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "FUNGI; BACTERIA/ARCHAEA; TERRESTRIAL ECOSYSTEMS; Antarctica", "locations": "Antarctica", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Fierer, Noah; Quandt, Alisha A; Adams, Byron; Lemonte, Joshua", "platforms": null, "repo": "EDI", "repositories": "EDI", "science_programs": null, "south": -90.0, "title": "Collaborative Research: ANT LIA Integrating Genomic and Phenotypic Analyses to understand Microbial Life in Antarctic Soils", "uid": "p0010414", "west": -180.0}, {"awards": "1937546 Morgan-Kiss, Rachael; 1937595 Briggs, Brandon", "bounds_geometry": "POLYGON((162 -77.616667,162.1 -77.616667,162.2 -77.616667,162.3 -77.616667,162.4 -77.616667,162.5 -77.616667,162.6 -77.616667,162.7 -77.616667,162.8 -77.616667,162.9 -77.616667,163 -77.616667,163 -77.6283336,163 -77.6400002,163 -77.6516668,163 -77.6633334,163 -77.67500000000001,163 -77.68666660000001,163 -77.69833320000001,163 -77.7099998,163 -77.7216664,163 -77.733333,162.9 -77.733333,162.8 -77.733333,162.7 -77.733333,162.6 -77.733333,162.5 -77.733333,162.4 -77.733333,162.3 -77.733333,162.2 -77.733333,162.1 -77.733333,162 -77.733333,162 -77.7216664,162 -77.7099998,162 -77.69833320000001,162 -77.68666660000001,162 -77.67500000000001,162 -77.6633334,162 -77.6516668,162 -77.6400002,162 -77.6283336,162 -77.616667))", "dataset_titles": "18S rRNA from McMurdo Dry Valley lakes", "datasets": [{"dataset_uid": "200436", "doi": "", "keywords": null, "people": null, "repository": "NCBI SRA", "science_program": null, "title": "18S rRNA from McMurdo Dry Valley lakes", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1125919/"}], "date_created": "Wed, 27 Jul 2022 00:00:00 GMT", "description": "Part I: Non-technical description: Microbial communities are of more than just a scientific curiosity. Microbes represent the single largest source of evolutionary and biochemical diversity on the planet. They are the major agents for cycling carbon, nitrogen, phosphorus, and other elements through the ecosystem. Despite their importance in ecosystem function, microbes are still generally overlooked in food web models and nutrient cycles. Moreover, microbes do not live in isolation: their growth and metabolism are influenced by complex interactions with other microorganisms. This project will focus on the ecology, activity and roles of microbial communities in Antarctic Lake ecosystems. The team will characterize the genetic underpinnings of microbial interactions and the influence of environmental gradients (e.g. light, nutrients, oxygen, sulfur) and seasons (e.g. summer vs. winter) on microbial networks in Lake Fryxell and Lake Bonney in the Taylor Valley within the McMurdo Dry Valley region. Finally, the project furthers the NSF goals of training new generations of scientists by including undergraduate and graduate students, a postdoctoral researcher and a middle school teacher in both lab and field research activities. This partnership will involve a number of other outreach training activities, including visits to classrooms and community events, participation in social media platforms, and webinars. Part II: Technical description: Ecosystem function in the extreme Antarctic Dry Valleys ecosystem is dependent on complex biogeochemical interactions between physiochemical environmental factors (e.g. light, nutrients, oxygen, sulfur), time of year (e.g. summer vs. winter) and microbes. Microbial network complexity can vary in relation to specific abiotic factors, which has important implications on the fragility and resilience of ecosystems under threat of environmental change. This project will evaluate the influence of biogeochemical factors on microbial interactions and network complexity in two Antarctic ice-covered lakes. The study will be structured by three main objectives: 1) infer positive and negative interactions from rich spatial and temporal datasets and investigate the influence of biogeochemical gradients on microbial network complexity using a variety of molecular approaches; 2) directly observe interactions among microbial eukaryotes and their partners using flow cytometry, single-cell sorting and microscopy; and 3) develop metabolic models of specific interactions using metagenomics. Outcomes from amplicon sequencing, meta-omics, and single-cell genomic approaches will be integrated to map specific microbial network complexity and define the role of interactions and metabolic activity onto trends in limnological biogeochemistry in different seasons. These studies will be essential to determine the relationship between network complexity and future climate conditions. Undergraduate researchers will be recruited from both an REU program with a track record of attracting underrepresented minorities and two minority-serving institutions. To further increase polar literacy training and educational impacts, the field team will include a teacher as part of a collaboration with the successful NSF-funded PolarTREC program and participation in activities designed for public outreach. 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The role of marine viruses on ocean productivity is largely understudied, especially in this global region. This team proposes to use combination of genomics, flow cytometry, and network modeling to test the hypothesis that viral biogeography, infection networks, and viral impacts on microbial metabolism can explain variations in net community production (NCP) and carbon cycling in the Southern Ocean. The project includes the training of a postdoctoral scholar, graduate students and undergraduate students. It also includes the development of a new Polar Sci ReachOut program in partnership with the University of Michigan Museum of Natural History especially targeted to middle-school students and teachers and the general public. The team will also produce a Science for Tomorrow (SFT) program for use in middle schools in metro-Detroit communities and lead a summer Research Experience for Teachers (RET) fellows. Part 2: Technical description: The study will leverage hundreds of existing samples collected for microbes and viruses from the Antarctic Circumpolar Expedition (ACE). These samples provide the first contiguous survey of viral diversity and microbial communities around Antarctica. Viral networks are being studied in the context of biogeochemical data to model community networks and predict net community production (NCP), which will provide a way to evaluate the role of viruses in Southern Ocean carbon cycling. Using cutting edge molecular and flow cytometry approaches, this project addresses the following questions: 1) How/why are Southern Ocean viral populations distributed across environmental gradients? 2a) Do viruses interfere with \"keystone\" metabolic pathways and biogeochemical processes of microbial communities in the Southern Ocean? 2b) Does nutrient availability or other environmental variables drive changes in virus-microbe infection networks in the Southern Ocean? Results will be used to develop and evaluate generative models of NCP predictions that incorporate the importance of viral traits and virus-host interactions. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "Southern Ocean; Amd/Us; AMD; FIELD INVESTIGATION; USA/NSF; AQUATIC SCIENCES; BACTERIA/ARCHAEA; MARINE ECOSYSTEMS; VIRUSES; USAP-DC", "locations": "Southern Ocean", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Duhaime, Melissa; Zaman, Luis", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION", "repositories": null, "science_programs": null, "south": -90.0, "title": "ANT LIA - Viral Ecogenomics of the Southern Ocean: Unifying Omics and Ecological Networks to Advance our Understanding of Antarctic Microbial Ecosystem Function", "uid": "p0010333", "west": -180.0}, {"awards": "1932876 Ball, Becky", "bounds_geometry": "POLYGON((-59.666116 -62.15,-59.5128377 -62.15,-59.3595594 -62.15,-59.2062811 -62.15,-59.0530028 -62.15,-58.8997245 -62.15,-58.7464462 -62.15,-58.5931679 -62.15,-58.4398896 -62.15,-58.2866113 -62.15,-58.133333 -62.15,-58.133333 -62.1731502,-58.133333 -62.1963004,-58.133333 -62.2194506,-58.133333 -62.2426008,-58.133333 -62.265751,-58.133333 -62.2889012,-58.133333 -62.3120514,-58.133333 -62.3352016,-58.133333 -62.3583518,-58.133333 -62.381502,-58.2866113 -62.381502,-58.4398896 -62.381502,-58.5931679 -62.381502,-58.7464462 -62.381502,-58.8997245 -62.381502,-59.0530028 -62.381502,-59.2062811 -62.381502,-59.3595594 -62.381502,-59.5128377 -62.381502,-59.666116 -62.381502,-59.666116 -62.3583518,-59.666116 -62.3352016,-59.666116 -62.3120514,-59.666116 -62.2889012,-59.666116 -62.265751,-59.666116 -62.2426008,-59.666116 -62.2194506,-59.666116 -62.1963004,-59.666116 -62.1731502,-59.666116 -62.15))", "dataset_titles": null, "datasets": null, "date_created": "Thu, 14 Apr 2022 00:00:00 GMT", "description": "Part I: Non-technical summary The Antarctic Peninsula warmed very rapidly in the late part of the 20th century, much faster than the global average, and this warming is predicted to resume and continue over the rest of the 21st century. One consequence of this rapid warming is the melting and subsequent retreat of glaciers, leading to an increase in newly-exposed land on the Peninsula that was previously covered with ice. Once new terrain is exposed, the process of ecological succession begins, with the arrival of early-colonizing plants, such as moss and lichens, and soil organisms - a process commonly referred to as the \u201cgreening\u201d of Antarctica. Early stages of succession will be an increasingly common feature on the Antarctic Peninsula, but the mechanisms by which they occur on the Antarctic continent is not well understood. Once the plants have established on the newly-exposed soil, they can change many important properties, such as water dynamics, nutrient recycling, soil development, and habitat for microscopic organisms, which will ultimately determine the structure and functioning of the future ecosystem as it develops. These relationships between vegetation, soil, and the associated microorganisms, referred to as \u201cplant-soil\u201d interactions, are something we know virtually nothing about. This project will be the first to make a comprehensive study of how the type of colonizing plant, and the expansion of those plants from climate change, will influence terrestrial ecosystems in Antarctica. Understanding these processes is critical to understanding how the greening Antarctica is occurring and how soil communities and processes are influenced by these expanding plant communities. Through this work the research team, will also be intensively training undergraduate and graduate students, including training of students from underrepresented groups and collaborative training of students from Chile and the US. Additionally, the research groups will continue their focus on scientific outreach with K-12 schools and the general public to expand awareness of the effects of climate change in Antarctica. Part II: Technical summary In this study, the researchers will use surveys across succession sites along the Antarctic Peninsula and Scotia Arc as well as a manipulative field experiment at glacier succession sites to test how species-specific plant functional traits impact soil properties and associated microbial and invertebrate communities in a greening Antarctica. In doing so, they will pursue three integrated aims to understand how Antarctic plant functional traits alter their soil environment and soil communities during succession after glacial retreat. AIM 1) Characterize six fundamental plant functional traits (thermal conductivity, water holding capacity, albedo, decomposability, tissue nutrient content, and secondary chemistry) among diverse Antarctica flora; AIM 2) Measure the relative effects of fundamental plant functional traits on soil physical properties and soil biogeochemistry across glacial succession gradients in Antarctica; and AIM 3) Measure the relative effects of fundamental plant functional traits on soil microbial and invertebrate communities across glacial succession gradients in Antarctica. They will explore how early-colonizing plants, especially mosses and lichens, alter soil physical, biogeochemical, and biological components, potentially impacting later patterns of succession. The researhcers will use intensive surveys of plant-soil interactions across succession sites and a manipulative transplant experiment in the South Shetland Islands, Antarctica to address their aims. The investigators will collect data on plant functional traits and their effects on soil physical properties, biogeochemistry, biotic abundance, and microbial metagenomics. The data collected will be the first comprehensive measures of the relative importance of plant functional types during glacial retreat and vegetative expansion from climate change in Antarctica, aiding our understanding of how plant functional group diversity and abundance are changing in a greening Antarctica. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": -58.133333, "geometry": "POINT(-58.8997245 -62.265751)", "instruments": null, "is_usap_dc": true, "keywords": "AMD; FIELD SURVEYS; ECOLOGICAL DYNAMICS; USA/NSF; SOIL CHEMISTRY; 25 De Mayo/King George Island; Antarctic Peninsula; PLANTS; Amd/Us; FUNGI; ANIMALS/INVERTEBRATES; USAP-DC; TERRESTRIAL ECOSYSTEMS; BACTERIA/ARCHAEA", "locations": "25 De Mayo/King George Island; Antarctic Peninsula", "north": -62.15, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Ball, Becky", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS", "repositories": null, "science_programs": null, "south": -62.381502, "title": "Collaborative Research: Exploring the Functional Role of Antarctic Plants during Terrestrial Succession", "uid": "p0010315", "west": -59.666116}, {"awards": "1341429 Ball, Becky", "bounds_geometry": "POLYGON((-68.205783 -60.706633,-65.9444531 -60.706633,-63.6831232 -60.706633,-61.4217933 -60.706633,-59.1604634 -60.706633,-56.8991335 -60.706633,-54.6378036 -60.706633,-52.3764737 -60.706633,-50.1151438 -60.706633,-47.8538139 -60.706633,-45.592484 -60.706633,-45.592484 -62.1204014,-45.592484 -63.5341698,-45.592484 -64.9479382,-45.592484 -66.3617066,-45.592484 -67.775475,-45.592484 -69.1892434,-45.592484 -70.6030118,-45.592484 -72.0167802,-45.592484 -73.4305486,-45.592484 -74.844317,-47.8538139 -74.844317,-50.1151438 -74.844317,-52.3764737 -74.844317,-54.6378036 -74.844317,-56.8991335 -74.844317,-59.1604634 -74.844317,-61.4217933 -74.844317,-63.6831232 -74.844317,-65.9444531 -74.844317,-68.205783 -74.844317,-68.205783 -73.4305486,-68.205783 -72.0167802,-68.205783 -70.6030118,-68.205783 -69.1892434,-68.205783 -67.775475,-68.205783 -66.3617066,-68.205783 -64.9479382,-68.205783 -63.5341698,-68.205783 -62.1204014,-68.205783 -60.706633))", "dataset_titles": "Climatic and environmental constraints on aboveground-belowground linkages and diversity across a latitudinal gradient in Antarctica", "datasets": [{"dataset_uid": "200289", "doi": "", "keywords": null, "people": null, "repository": "OSF - Center for Open Science", "science_program": null, "title": "Climatic and environmental constraints on aboveground-belowground linkages and diversity across a latitudinal gradient in Antarctica", "url": "https://osf.io/8xfrc/"}], "date_created": "Thu, 14 Apr 2022 00:00:00 GMT", "description": "The Antarctic Peninsula is experiencing rapid environmental changes, which will influence the community of organisms that live there. However, we know very little about the microscopic organisms living in the soil in this region. Soil biology (including bacteria, fungi, and invertebrates) are responsible for many important processes that sustain ecosystems, such as nutrient recycling. Without understanding the environmental conditions that influence soil biodiversity along the Antarctic Peninsula, our ability to predict the consequences of global change is strongly limited. This project will identify the soil community at many sites along the Antarctic Peninsula to discover how the community changes with environmental conditions from north to south. The project will also identify how the soil community at each site differs under different types of plants. Understanding more about the ways in which plant cover and climate conditions influence soil biodiversity will allow predictions of how communities will respond to future changes such as climate warming and invasive plant species. The project will also further the NSF goals of making scientific discoveries available to the general public and of training new generations of scientists. The investigators will engage with outreach to K-12 students and the general public both directly and through a blog and will participate in workshops for K-12 teachers. Additionally, the project will provide the opportunity for many undergraduate and graduate students of diverse backgrounds to be trained in interdisciplinary research. The investigators will determine the nature and strength of plant-soil linkages in influencing soil community composition and diversity over a latitudinal gradient of environmental and climatic conditions. The goals are to (1) increase our understanding of current biogeography and diversity by providing in-depth knowledge of soil community composition and complexity as it relates to environmental and climatic characteristics; and (2) determine the nature of aboveground-belowground community linkages over varying spatial scales. The team will identify the composition and diversity of soil communities under key habitat types (grass, moss, algae, etc.). Microbial communities (bacteria, fungi, archaea) will be investigated using pyrosequencing for community composition analysis and metagenomic sequencing to identify functional capabilities. Invertebrates (nematodes, tardigrades, rotifers, microarthropods) will be extracted and identified to the lowest possible taxonomic level. Soil chemistry (pH, nutrient content, soil moisture, etc.) and climate conditions will be measured to determine the relationship between soil communities and physical and chemical properties. Structural equation modeling will be used to identify aboveground-belowground linkage pathways and quantify link strengths under varying environmental conditions.", "east": -45.592484, "geometry": "POINT(-56.8991335 -67.775475)", "instruments": null, "is_usap_dc": true, "keywords": "FUNGI; FIELD INVESTIGATION; AMD; Amd/Us; TERRESTRIAL ECOSYSTEMS; USA/NSF; ANIMALS/INVERTEBRATES; SOIL CHEMISTRY; BACTERIA/ARCHAEA; Antarctic Peninsula; ECOSYSTEM FUNCTIONS; USAP-DC", "locations": "Antarctic Peninsula", "north": -60.706633, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Ball, Becky; Van Horn, David", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION", "repo": "OSF - Center for Open Science", "repositories": "OSF - Center for Open Science", "science_programs": null, "south": -74.844317, "title": "Collaborative Research: Climatic and Environmental Constraints on Aboveground-Belowground Linkages and Diversity across a Latitudinal Gradient in Antarctica", "uid": "p0010314", "west": -68.205783}, {"awards": "2046800 Thurber, Andrew", "bounds_geometry": "POLYGON((162 -77,162.6 -77,163.2 -77,163.8 -77,164.4 -77,165 -77,165.6 -77,166.2 -77,166.8 -77,167.4 -77,168 -77,168 -77.1,168 -77.2,168 -77.3,168 -77.4,168 -77.5,168 -77.6,168 -77.7,168 -77.8,168 -77.9,168 -78,167.4 -78,166.8 -78,166.2 -78,165.6 -78,165 -78,164.4 -78,163.8 -78,163.2 -78,162.6 -78,162 -78,162 -77.9,162 -77.8,162 -77.7,162 -77.6,162 -77.5,162 -77.4,162 -77.3,162 -77.2,162 -77.1,162 -77))", "dataset_titles": null, "datasets": null, "date_created": "Sat, 21 Aug 2021 00:00:00 GMT", "description": "Part I: Non-technical description: Methane is one of the more effective atmospheric gases at retaining heat in the lower atmosphere and the earth\u2019s crust contains large quantities of methane. Research that identifies the factors that control methane\u2019s release into the atmosphere is critical to understanding and mitigating climate change. One of the most effective natural processes that inhibits the release of methane from aquatic habitats is a community of bacteria and Archaea (microbes) that use the chemical energy stored in methane, transforming methane into less-climate-sensitive compounds. The amount of methane that may be released in Antarctica is unknown, and it is unclear which microbes consume the methane before it is released from the ocean in Antarctica. This project will study one of the few methane seeps known in Antarctica to advance our understanding of which microbes inhibit the release of methane in marine environments. The research will also identify if methane is a source of energy for other Antarctic organisms. The researchers will analyze the microbial species associated with methane consumption over several years of field and laboratory research based at an Antarctic US station, McMurdo. This project clearly expands the fundamental knowledge of Antarctic systems, biota, and processes outlined as a goal in the Antarctic solicitation. This research communicates and produces educational material for K-12, college, and graduate students to inspire and inform the public about the role Antarctic ecosystems play in the global environment. This project also provides a young professor an opportunity to establish himself as an expert in the field of Antarctic microbial ecology to help solidify his academic career. Part II: Technical description: Microbes act as filter to methane release from the ocean into the atmosphere, where microbial chemosynthetic production harvests the chemical energy stored in this greenhouse gas. In spite of methane reservoirs in Antarctica being as large as Arctic permafrost, we know only a little about the taxa or dominant processes involved in methane consumption in Antarctica. The principal investigator will undertake a genomic and transcriptomic study of microbial communities developed and still developing after initiation of methane seepage in McMurdo Sound. An Antarctic methane seep was discovered at this location in 2012 after it began seeping in 2011. Five years after it began releasing methane, the methane-oxidizing microbial community was underdeveloped and methane was still escaping from the seafloor. This project will be essential in elucidating the response of microbial communities to methane release and identify how methane oxidation occurs within the constraints of the low polar temperatures. This investigation is based on 4 years of field sampling and will establish a time series of the development of cold seep microbial communities in Antarctica. A genome-to-ecosystem approach will establish how the Southern Ocean microbial community is adapted to prevent methane release into the ocean. As methane is an organic carbon source, results from this study will have implications for the Southern Ocean carbon cycle. Two graduate students will be trained and supported with undergraduates participating in laboratory activities. The researcher aims to educate, inspire and communicate about Antarctic methane seeps to a broad community. A mixed-media approach, with videos, art and education in schools will be supported in collaboration with a filmmaker, teachers and a visual artist. Students will be trained in filmmaking and K-12 students from under-represented communities will be introduced to Antarctic science through visual arts. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 168.0, "geometry": "POINT(165 -77.5)", "instruments": null, "is_usap_dc": true, "keywords": "AMD; USA/NSF; USAP-DC; BACTERIA/ARCHAEA; McMurdo Sound; BENTHIC; FIELD SURVEYS; Amd/Us; ECOSYSTEM FUNCTIONS", "locations": "McMurdo Sound", "north": -77.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Thurber, Andrew", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS", "repositories": null, "science_programs": null, "south": -78.0, "title": "CAREER: Ecosystem Impacts of Microbial Succession and Production at Antarctic Methane Seeps", "uid": "p0010250", "west": 162.0}, {"awards": "1846837 Bowman, Jeff", "bounds_geometry": null, "dataset_titles": "PRJNA894514; PRJNA901488; Raw flow cytometry data from Palmer Peninsula", "datasets": [{"dataset_uid": "200480", "doi": "", "keywords": null, "people": null, "repository": "NCBI SRA", "science_program": null, "title": "PRJNA901488", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA901488"}, {"dataset_uid": "601930", "doi": "10.15784/601930", "keywords": "Antarctica; Cryosphere; Palmer Station", "people": "Bowman, Jeff; Connors, Elizabeth", "repository": "USAP-DC", "science_program": null, "title": "Raw flow cytometry data from Palmer Peninsula", "url": "https://www.usap-dc.org/view/dataset/601930"}, {"dataset_uid": "200479", "doi": "", "keywords": null, "people": null, "repository": "NCBI SRA", "science_program": null, "title": "PRJNA894514", "url": "https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA894514"}], "date_created": "Fri, 25 Jun 2021 00:00:00 GMT", "description": "The coastal Antarctic is undergoing great environmental change. Physical changes in the environment, such as altered sea ice duration and extent, have a direct impact on the phytoplankton and bacteria species which form the base of the marine foodweb. Photosynthetic phytoplankton are the ocean\u0027s primary producers, transforming (fixing) CO2 into organic carbon molecules and providing a source of food for zooplankton and larger predators. When phytoplankton are consumed by zooplankton, or killed by viral attack, they release large amounts of organic carbon and nutrients into the environment. Heterotrophic bacteria must eat other things, and function as \"master recyclers\", consuming these materials and converting them to bacterial biomass which can feed larger organisms such as protists. Some protists are heterotrophs, but others are mixotrophs, able to grow by photosynthesis or heterotrophy. Previous work suggests that by killing and eating bacteria, protists and viruses may regulate bacterial populations, but how these processes are regulated in Antarctic waters is poorly understood. This project will use experiments to determine the rate at which Antarctic protists consume bacteria, and field studies to identify the major bacterial taxa involved in carbon uptake and recycling. In addition, this project will use new sequencing technology to obtain completed genomes for many Antarctic marine bacteria. To place this work in an ecosystem context this project will use microbial diversity data to inform rates associated with key microbial processes within the PALMER ecosystem model. This project addresses critical unknowns regarding the ecological role of heterotrophic marine bacteria in the coastal Antarctic and the top-down controls on bacterial populations. Previous work suggests that at certain times of the year grazing by heterotrophic and mixotrophic protists may meet or exceed bacterial production rates. Similarly, in more temperate waters bacteriophages (viruses) are thought to contribute significantly to bacterial mortality during the spring and summer. These different top-down controls have implications for carbon flow through the marine foodweb, because protists are grazed more efficiently by higher trophic levels than are bacteria. This project will use a combination of grazing experiments and field observations to assess the temporal dynamics of mortality due to temperate bacteriophage and protists. Although many heterotrophic bacterial strains observed in the coastal Antarctic are taxonomically similar to strains from other regions, recent work suggest that they are phylogenetically and genetically distinct. To better understand the ecological function and evolutionary trajectories of key Antarctic marine bacteria, their genomes will be isolated and sequenced. Then, these genomes will be used to improve the predictions of the paprica metabolic inference pipeline, and our understanding of the relationship between heterotrophic bacteria and their major predators in the Antarctic marine environment. Finally, researchers will modify the Regional Test-Bed Model model to enable microbial diversity data to be used to optimize the starting conditions of key parameters, and to constrain the model\u0027s data assimilation methods. There is an extensive education and outreach component to this project that is designed to engage students and the public in diverse activities centered on Antarctic microbiota and marine sciences. A new module on Antarctic marine science will be developed for the popular Sally Ride Science program, and two existing undergraduate courses at UC San Diego will be strengthened with laboratory modules introducing emerging technology, and with cutting-edge polar science. A PhD student and a post-doctoral researcher will be supported by this project. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "Magmatic Volatiles; BACTERIA/ARCHAEA; VIRUSES; USA/NSF; Palmer Station; ECOSYSTEM FUNCTIONS; COMMUNITY DYNAMICS; LABORATORY; Amd/Us; PROTISTS; AMD; USAP-DC", "locations": "Palmer Station", "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Bowman, Jeff; Connors, Elizabeth", "platforms": "OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repo": "NCBI SRA", "repositories": "NCBI SRA; USAP-DC", "science_programs": null, "south": null, "title": "CAREER: Understanding microbial heterotrophic processes in coastal Antarctic waters", "uid": "p0010201", "west": null}, {"awards": "1643466 Hollibaugh, James; 1643345 Popp, Brian", "bounds_geometry": "POLYGON((-78.20206667 -64.03195833,-76.785055836 -64.03195833,-75.368045002 -64.03195833,-73.951034168 -64.03195833,-72.534023334 -64.03195833,-71.1170125 -64.03195833,-69.700001666 -64.03195833,-68.282990832 -64.03195833,-66.865979998 -64.03195833,-65.448969164 -64.03195833,-64.03195833 -64.03195833,-64.03195833 -64.554377497,-64.03195833 -65.076796664,-64.03195833 -65.599215831,-64.03195833 -66.121634998,-64.03195833 -66.644054165,-64.03195833 -67.166473332,-64.03195833 -67.688892499,-64.03195833 -68.211311666,-64.03195833 -68.733730833,-64.03195833 -69.25615,-65.448969164 -69.25615,-66.865979998 -69.25615,-68.282990832 -69.25615,-69.700001666 -69.25615,-71.1170125 -69.25615,-72.534023334 -69.25615,-73.951034168 -69.25615,-75.368045002 -69.25615,-76.785055836 -69.25615,-78.20206667 -69.25615,-78.20206667 -68.733730833,-78.20206667 -68.211311666,-78.20206667 -67.688892499,-78.20206667 -67.166473332,-78.20206667 -66.644054165,-78.20206667 -66.121634998,-78.20206667 -65.599215831,-78.20206667 -65.076796664,-78.20206667 -64.554377497,-78.20206667 -64.03195833))", "dataset_titles": "\"Collaborative research: Chemoautotrophy in Antarctic bacterioplankton communities supported by the oxidation of urea-derived nitrogen\"; Expedition data of LMG1801", "datasets": [{"dataset_uid": "200193", "doi": "Not yet assigned", "keywords": null, "people": null, "repository": "BCO-DMO", "science_program": null, "title": "\"Collaborative research: Chemoautotrophy in Antarctic bacterioplankton communities supported by the oxidation of urea-derived nitrogen\"", "url": "https://www.bco-dmo.org/project/775717"}, {"dataset_uid": "200124", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG1801", "url": "https://www.rvdata.us/search/cruise/LMG1801"}], "date_created": "Fri, 18 Dec 2020 00:00:00 GMT", "description": "Part 1: Nitrification is the conversion of ammonium to nitrate by a two-step process involving two different guilds of microorganisms: ammonia- and nitrite-oxidizers. The process is central to the global nitrogen cycle, affecting everything from retention of fertilizer on croplands to removal of excess nitrogen from coastal waters before it can cause blooms of harmful algae. It also produces nitrous oxide, an ozone-destroying, greenhouse gas. The energy derived from both steps of nitrification is used to convert inorganic carbon into microbial biomass. The biomass produced contributes to the overall food web production of the Southern Ocean and may be a particularly important subsidy during winter when low light levels restrict the other major source of biomass, primary production by single-celled plants. This project addresses three fundamental questions about the biology and geochemistry of polar oceans, with a focus on the process of nitrification. The first question the project will address concerns the contribution of chemoautotrophy (based on nitrification) to the overall supply of organic carbon to the food web of the Southern Ocean. Previous measurements indicate that it contributes about 9% to the Antarctic food web on an annual basis, but those measurements did not include the additional production associated with nitrite oxidation. The second question to be addressed is related to the first and concerns the coupling between the steps of the process. The third seeks to determine the significance of the contribution of other sources of nitrogen, (specifically organic nitrogen and urea released by other organisms) to nitrification because these contributions may not be assessed by standard protocols. Measurements made by others suggest that urea in particular might be as important as ammonium to nitrification in polar regions. This project will result in training a postdoctoral researcher and provide undergraduate students opportunities to gain hand-on experience with research on microbial geochemistry. The Palmer LTER (PAL) activities have focused largely on the interaction between ocean climate and the marine food web affecting top predators. Relatively little effort has been devoted to studying processes related to the microbial geochemistry of nitrogen cycling as part of the Palmer Long Term Ecological Research (LTER) program, yet these are a major themes at other sites. This work will contribute substantially to understanding an important aspect of nitrogen cycling and bacterioplankton production in the PAL-LTER study area. The team will be working synergistically and be participating fully in the education and outreach efforts of the Palmer LTER, including making highlights of the findings available for posting to their project web site and participating in any special efforts they have in the area of outreach. Part 2: The proposed work will quantify oxidation rates of 15N supplied as ammonium, urea and nitrite, allowing us to estimate the contribution of urea-derived N and complete nitrification (ammonia to nitrate) to chemoautotrophy and bacterioplankton production in Antarctic coastal waters. The project will compare these estimates to direct measurements of the incorporation of 14C into organic matter the dark for an independent estimate of chemoautotrophy. The team aims to collect samples spanning the water column: from surface water (~10 m), winter water (50-100 m) and circumpolar deep water (\u003e150 m); on a cruise surveying the continental shelf and slope west of the Antarctic Peninsula in the austral summer of 2018. Other samples will be taken to measure the concentrations of nitrate, nitrite, ammonia and urea, for qPCR analysis of the abundance of relevant microorganisms, and for studies of related processes. The project will rely on collaboration with the existing Palmer LTER to ensure that ancillary data (bacterioplankton abundance and production, chlorophyll, physical and chemical variables) will be available. The synergistic activities of this project along with the LTER activities will provide a unique opportunity to assess chemoautotrophy in context of the overall ecosystem?s dynamics- including both primary and secondary production processes.", "east": -64.03195833, "geometry": "POINT(-71.1170125 -66.644054165)", "instruments": null, "is_usap_dc": true, "keywords": "Pal-Lter; NITROGEN; SHIPS; USAP-DC; MARINE ECOSYSTEMS; BACTERIA/ARCHAEA; BIOGEOCHEMICAL CYCLES; Amd/Us; West Antarctic Shelf; USA/NSF; AMD", "locations": "West Antarctic Shelf; Pal-Lter", "north": -64.03195833, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Hollibaugh, James T.; Popp, Brian", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e SHIPS", "repo": "BCO-DMO", "repositories": "BCO-DMO; R2R", "science_programs": null, "south": -69.25615, "title": "Collaborative Research: Chemoautotrophy in Antarctic Bacterioplankton Communities Supported by the Oxidation of Urea-derived Nitrogen", "uid": "p0010150", "west": -78.20206667}, {"awards": "1620976 Johnson, Sarah", "bounds_geometry": "POLYGON((160 -77,160.3 -77,160.6 -77,160.9 -77,161.2 -77,161.5 -77,161.8 -77,162.1 -77,162.4 -77,162.7 -77,163 -77,163 -77.1,163 -77.2,163 -77.3,163 -77.4,163 -77.5,163 -77.6,163 -77.7,163 -77.8,163 -77.9,163 -78,162.7 -78,162.4 -78,162.1 -78,161.8 -78,161.5 -78,161.2 -78,160.9 -78,160.6 -78,160.3 -78,160 -78,160 -77.9,160 -77.8,160 -77.7,160 -77.6,160 -77.5,160 -77.4,160 -77.3,160 -77.2,160 -77.1,160 -77))", "dataset_titles": "GenBank Sequence Read Archive with accession numbers SRR8217969 - SRR8217976 and project accession PRJNA506221", "datasets": [{"dataset_uid": "200164", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "GenBank Sequence Read Archive with accession numbers SRR8217969 - SRR8217976 and project accession PRJNA506221", "url": "https://www.ncbi.nlm.nih.gov/bioproject/PRJNA506221/"}], "date_created": "Tue, 01 Sep 2020 00:00:00 GMT", "description": "Despite recent advances, we still know little about how life and its traces persist in extremely harsh conditions. What survival strategies do cells employ when pushed to their limit? Using a new technique, this project will investigate whether Antarctic paleolakes harbor \"microbial seed banks,\" or caches of viable microbes adapted to past paleoenvironments that could help transform our understanding of how cells survive over ancient timescales. Findings from this investigation could also illuminate novel DNA repair pathways with possible biomedical and biotechnology applications and help to refine life detection strategies for Mars. The project will bring Antarctic research to Georgetown University\u0027s campus for the first time, providing training opportunities in cutting edge analytical techniques for multiple students and a postdoctoral fellow. The field site will be the McMurdo Dry Valleys, which provide an unrivaled opportunity to investigate fundamental questions about the persistence of microbial life. Multiple lines of evidence, from interbedded and overlying ashfall deposits to parameterized models, suggest that the large-scale landforms there have remained essentially fixed as far back as the middle of the Miocene Epoch (i.e., ~8 million years ago). This geologic stability, coupled with geographic isolation and a steady polar climate, mean that biological activity has probably undergone few qualitative changes over the last one to two million years. The team will sample paleolake facies using sterile techniques from multiple Dry Valleys sites and extract DNA from entombed organic material. Genetic material will then be sequenced using Pacific Biosciences\u0027 Single Molecule, Real-Time DNA sequencing technology, which sequences native DNA as opposed to amplified DNA, thereby eliminating PCR primer bias, and enables read lengths that have never before been possible. The data will be analyzed with a range of bioinformatic techniques, with results that stand to impact our understanding of cell biology, Antarctic paleobiology, microbiology and biogeography, biotechnology, and planetary science.", "east": 163.0, "geometry": "POINT(161.5 -77.5)", "instruments": null, "is_usap_dc": true, "keywords": "ECOSYSTEM FUNCTIONS; COMMUNITY DYNAMICS; BACTERIA/ARCHAEA; CYANOBACTERIA (BLUE-GREEN ALGAE); LABORATORY; Dry Valleys", "locations": "Dry Valleys", "north": -77.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Earth Sciences", "paleo_time": null, "persons": "Johnson, Sarah", "platforms": "OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repo": "NCBI GenBank", "repositories": "NCBI GenBank", "science_programs": null, "south": -78.0, "title": "EAGER: Single-Molecule DNA Sequencing of Antarctic Paleolakes", "uid": "p0010125", "west": 160.0}, {"awards": "1543405 Leventer, Amy; 1543441 Fricker, Helen; 1543347 Rosenheim, Brad; 1543396 Christner, Brent; 1543453 Lyons, W. Berry; 1543537 Priscu, John", "bounds_geometry": "POLYGON((-163.611 -84.33543,-162.200034 -84.33543,-160.789068 -84.33543,-159.378102 -84.33543,-157.967136 -84.33543,-156.55617 -84.33543,-155.145204 -84.33543,-153.734238 -84.33543,-152.323272 -84.33543,-150.912306 -84.33543,-149.50134 -84.33543,-149.50134 -84.3659157,-149.50134 -84.3964014,-149.50134 -84.4268871,-149.50134 -84.4573728,-149.50134 -84.4878585,-149.50134 -84.5183442,-149.50134 -84.5488299,-149.50134 -84.5793156,-149.50134 -84.6098013,-149.50134 -84.640287,-150.912306 -84.640287,-152.323272 -84.640287,-153.734238 -84.640287,-155.145204 -84.640287,-156.55617 -84.640287,-157.967136 -84.640287,-159.378102 -84.640287,-160.789068 -84.640287,-162.200034 -84.640287,-163.611 -84.640287,-163.611 -84.6098013,-163.611 -84.5793156,-163.611 -84.5488299,-163.611 -84.5183442,-163.611 -84.4878585,-163.611 -84.4573728,-163.611 -84.4268871,-163.611 -84.3964014,-163.611 -84.3659157,-163.611 -84.33543))", "dataset_titles": "Antarctica - PI Continuous - GZ01-WIS_GroundingZone_01 P.S. - GPS/GNSS Observations Dataset; Antarctica - PI Continuous - GZ13-WIS_GroundingZone_13 P.S. - GPS/GNSS Observations Dataset; Antarctica - PI Continuous - LA02-WIS_LAKES_02 P.S. - GPS/GNSS Observations Dataset; Antarctica - PI Continuous - LA06-WIS_LAKES_06 P.S. - GPS/GNSS Observations Dataset; Antarctica - PI Continuous - LA07-WIS_LAKES_07 P.S. - GPS/GNSS Observations Dataset; Antarctica - PI Continuous - LA09-WIS_LAKES_09 P.S. - GPS/GNSS Observations Dataset; Bistatic Radar Sounding of Whillans Ice Stream, Antarctica and Store Glacier, Greenland; CTD data from Mercer Subglacial Lake and access borehole; Discrete bulk sediment properties data from Mercer Subglacial Lake; Isotopic data from Whillans Ice Stream grounding zone, West Antarctica; Mercer Subglacial Lake radiocarbon and stable isotope data ; Mercer Subglacial Lake (SLM) microbial composition: 16S rRNA genes (Sequence Read Archive; BioProject: PRJNA790995); Mercer Subglacial Lake (SLM) noble gas and isotopic data; Mercer Subglacial Lake water column viral metagenomic sequencing; Salsa sediment cores; Sediment porewater properties data from Mercer Subglacial Lake; Water column biogeochemical data from Mercer Subglacial Lake", "datasets": [{"dataset_uid": "200342", "doi": null, "keywords": null, "people": null, "repository": "GenBank", "science_program": null, "title": "Mercer Subglacial Lake water column viral metagenomic sequencing", "url": "https://www.ncbi.nlm.nih.gov/biosample/32811410"}, {"dataset_uid": "200246", "doi": "", "keywords": null, "people": null, "repository": "OSU-MGR", "science_program": null, "title": "Salsa sediment cores", "url": "https://osu-mgr.org"}, {"dataset_uid": "601657", "doi": "10.15784/601657", "keywords": "Antarctica; Conductivity; CTD; Depth; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Hot Water Drill; Mercer Subglacial Lake; Physical Properties; SALSA; Subglacial Lake; Temperature", "people": "Leventer, Amy; Dore, John; Priscu, John; Rosenheim, Brad", "repository": "USAP-DC", "science_program": null, "title": "CTD data from Mercer Subglacial Lake and access borehole", "url": "https://www.usap-dc.org/view/dataset/601657"}, {"dataset_uid": "601661", "doi": "10.15784/601661", "keywords": "Antarctica; Carbon; Glacier; Glaciers/ice Sheet; Glaciers/Ice Sheet; Iron; Mercer Subglacial Lake; Mineralogy; Particle Size; Physical Properties; SALSA; Sediment Core; Sulfur; West Antarctic Ice Sheet", "people": "Dore, John; Skidmore, Mark; Campbell, Timothy; Michaud, Alexander; Hawkings, Jon; Science Team, SALSA; Venturelli, Ryan A; Tranter, Martyn", "repository": "USAP-DC", "science_program": null, "title": "Discrete bulk sediment properties data from Mercer Subglacial Lake", "url": "https://www.usap-dc.org/view/dataset/601661"}, {"dataset_uid": "601663", "doi": "10.15784/601663", "keywords": "Antarctica; Carbon; Cell Counts; Geochemistry; Glacier; Glaciers/ice Sheet; Glaciers/Ice Sheet; Mercer Subglacial Lake; Microbes; Nutrients; SALSA; Stable Isotopes; Trace Elements; West Antarctic Ice Sheet", "people": "Hawkings, Jon; Skidmore, Mark; Dore, John; Science Team, SALSA; Priscu, John; Tranter, Martyn; Barker, Joel; Li, Wei; Steigmeyer, August", "repository": "USAP-DC", "science_program": null, "title": "Water column biogeochemical data from Mercer Subglacial Lake", "url": "https://www.usap-dc.org/view/dataset/601663"}, {"dataset_uid": "200212", "doi": "10.7283/PT0Q-JB95", "keywords": null, "people": null, "repository": "UNAVCO", "science_program": null, "title": "Antarctica - PI Continuous - GZ01-WIS_GroundingZone_01 P.S. - GPS/GNSS Observations Dataset", "url": "https://www.unavco.org/data/doi/10.7283/PT0Q-JB95"}, {"dataset_uid": "200213", "doi": "10.7283/F7BB-JH05", "keywords": null, "people": null, "repository": "UNAVCO", "science_program": null, "title": "Antarctica - PI Continuous - GZ13-WIS_GroundingZone_13 P.S. - GPS/GNSS Observations Dataset", "url": "https://www.unavco.org/data/doi/10.7283/F7BB-JH05"}, {"dataset_uid": "200282", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Mercer Subglacial Lake (SLM) microbial composition: 16S rRNA genes (Sequence Read Archive; BioProject: PRJNA790995)", "url": "https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA790995"}, {"dataset_uid": "601664", "doi": "10.15784/601664", "keywords": "Antarctica; Gas; Geochemistry; Glacier; Glaciology; Mercer Subglacial Lake; Methane; SALSA; Sediment Core; West Antarctic Ice Sheet", "people": "Dore, John; Steigmeyer, August; Tranter, Martyn; Science Team, SALSA; Skidmore, Mark; Michaud, Alexander", "repository": "USAP-DC", "science_program": null, "title": "Sediment porewater properties data from Mercer Subglacial Lake", "url": "https://www.usap-dc.org/view/dataset/601664"}, {"dataset_uid": "601672", "doi": "10.15784/601672", "keywords": "Antarctica; Isotope; Mercer Subglacial Lake; Radiocarbon; Subglacial Lake", "people": "Rosenheim, Brad; Venturelli, Ryan", "repository": "USAP-DC", "science_program": null, "title": "Mercer Subglacial Lake radiocarbon and stable isotope data ", "url": "https://www.usap-dc.org/view/dataset/601672"}, {"dataset_uid": "601472", "doi": "10.15784/601472", "keywords": "Antarctica; Bistatic Radar; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; GPS Data; Greenland; Lake Whillans; Radar; Store Glacier; Whillans Ice Stream; WISSARD", "people": "MacKie, Emma; Christoffersen, Poul; Bienert, Nicole; Schroeder, Dustin; Siegfried, Matthew; Peters, Sean; Dawson, Eliza", "repository": "USAP-DC", "science_program": "WISSARD", "title": "Bistatic Radar Sounding of Whillans Ice Stream, Antarctica and Store Glacier, Greenland", "url": "https://www.usap-dc.org/view/dataset/601472"}, {"dataset_uid": "601498", "doi": "10.15784/601498", "keywords": "Antarctica; Mercer Subglacial Lake; Noble Gas", "people": "Gardner, Christopher B.; Lyons, W. Berry", "repository": "USAP-DC", "science_program": null, "title": "Mercer Subglacial Lake (SLM) noble gas and isotopic data", "url": "https://www.usap-dc.org/view/dataset/601498"}, {"dataset_uid": "200217", "doi": "10.7283/3JMY-Y504", "keywords": null, "people": null, "repository": "UNAVCO", "science_program": null, "title": "Antarctica - PI Continuous - LA09-WIS_LAKES_09 P.S. - GPS/GNSS Observations Dataset", "url": "https://www.unavco.org/data/doi/10.7283/3JMY-Y504"}, {"dataset_uid": "200216", "doi": "10.7283/F8NH-CV04", "keywords": null, "people": null, "repository": "UNAVCO", "science_program": null, "title": "Antarctica - PI Continuous - LA07-WIS_LAKES_07 P.S. - GPS/GNSS Observations Dataset", "url": "https://www.unavco.org/data/doi/10.7283/F8NH-CV04"}, {"dataset_uid": "200215", "doi": "10.7283/C503-KS23", "keywords": null, "people": null, "repository": "UNAVCO", "science_program": null, "title": "Antarctica - PI Continuous - LA06-WIS_LAKES_06 P.S. - GPS/GNSS Observations Dataset", "url": "https://www.unavco.org/data/doi/10.7283/C503-KS23"}, {"dataset_uid": "200214", "doi": "10.7283/YW8Z-TK03", "keywords": null, "people": null, "repository": "UNAVCO", "science_program": null, "title": "Antarctica - PI Continuous - LA02-WIS_LAKES_02 P.S. - GPS/GNSS Observations Dataset", "url": "https://www.unavco.org/data/doi/10.7283/YW8Z-TK03"}, {"dataset_uid": "601360", "doi": "10.15784/601360", "keywords": "Antarctica; Radiocarbon; Sediment; Whillans Ice Stream", "people": "Venturelli, Ryan A", "repository": "USAP-DC", "science_program": "WISSARD", "title": "Isotopic data from Whillans Ice Stream grounding zone, West Antarctica", "url": "https://www.usap-dc.org/view/dataset/601360"}], "date_created": "Thu, 16 Jul 2020 00:00:00 GMT", "description": "The Antarctic subglacial environment remains one of the least explored regions on Earth. This project will examine the physical and biological characteristics of Subglacial Lake Mercer, a lake that lies 1200m beneath the West Antarctic Ice Sheet. This study will address key questions relating to the stability of the ice sheet, the subglacial hydrological system, and the deep-cold subglacial biosphere. The education and outreach component aims to widely disseminate results to the scientific community and to the general public through short films, a blog, and a website. Subglacial Lake Mercer is one of the larger hydrologically active lakes in the southern basin of the Whillans Ice Plain, West Antarctica. It receives about 25 percent of its water from East Antarctica with the remainder originating from West Antarctica, is influenced by drain/fill cycles in a lake immediately upstream (Subglacial Lake Conway), and lies about 100 km upstream of the present grounding line of the Ross Ice Shelf. This site will yield information on the history of the Whillans and Mercer Ice Streams, and on grounding line migration. The integrated study will include direct sampling of basal ice, water, and sediment from the lake in concert with surface geophysical surveys over a three-year period to define the hydrological connectivity among lakes on the Whillans Ice Plain and their flow paths to the sea. The geophysical surveys will furnish information on subglacial hydrology, aid the site selection for hot-water drilling, and provide spatial context for interpreting findings. The hot-water-drilled boreholes will be used to collect basal ice samples, provide access for direct measurement of subglacial physical, chemical, and biological conditions in the water column and sediments, and to explore the subglacial water cavities using a remotely operated vehicle equipped with sensors, cameras, and sampling equipment. Data collected from this study will address the overarching hypothesis \"Contemporary biodiversity and carbon cycling in hydrologically-active subglacial environments associated with the Mercer and Whillans ice streams are regulated by the mineralization and cycling of relict marine organic matter and through interactions among ice, rock, water, and sediments\". The project will be undertaken by a collaborative team of scientists, with expertise in microbiology, biogeochemistry, hydrology, geophysics, glaciology, marine geology, paleoceanography, and science communication.", "east": -149.50134, "geometry": "POINT(-156.55617 -84.4878585)", "instruments": null, "is_usap_dc": true, "keywords": "SEDIMENTS; Antarctica; ISOTOPES; Subglacial Lake; USAP-DC; VIRUSES; PALEOCLIMATE RECONSTRUCTIONS; BACTERIA/ARCHAEA; LABORATORY; Radiocarbon; Whillans Ice Stream; AMD; SALSA; ECOSYSTEM FUNCTIONS; RADIOCARBON; FIELD INVESTIGATION; ICE MOTION; Mercer Ice Stream; Amd/Us; USA/NSF; GLACIERS/ICE SHEETS", "locations": "Antarctica; Mercer Ice Stream; Whillans Ice Stream", "north": -84.33543, "nsf_funding_programs": "Antarctic Glaciology; Antarctic Integrated System Science; Antarctic Glaciology; Antarctic Glaciology; Antarctic Integrated System Science; Antarctic Integrated System Science; Antarctic Integrated System Science; Antarctic Glaciology; Antarctic Glaciology; Antarctic Integrated System Science; Antarctic Instrumentation and Support; Antarctic Integrated System Science", "paleo_time": null, "persons": "Rosenheim, Brad; Fricker, Helen; Priscu, John; Leventer, Amy; Dore, John; Lyons, W. Berry; Christner, Brent", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION; OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repo": "GenBank", "repositories": "GenBank; NCBI GenBank; OSU-MGR; UNAVCO; USAP-DC", "science_programs": null, "south": -84.640287, "title": "Collaborative Research: Subglacial Antarctic Lakes Scientific Access (SALSA): Integrated Study of Carbon Cycling in Hydrologically-active Subglacial Environments", "uid": "p0010119", "west": -163.611}, {"awards": "1642570 Thurber, Andrew", "bounds_geometry": "POINT(166.666 -77.8)", "dataset_titles": "Microbial community composition of the Cinder Cones Cold Seep", "datasets": [{"dataset_uid": "200035", "doi": "DOI:10.1575/1912/bco-dmo.756997.1", "keywords": null, "people": null, "repository": "BCO-DMO", "science_program": null, "title": "Microbial community composition of the Cinder Cones Cold Seep", "url": "https://www.bco-dmo.org/dataset/756997"}], "date_created": "Fri, 24 May 2019 00:00:00 GMT", "description": "Methane is a potent greenhouse gas that is naturally emitted into the oceans by geologic seeps and microbial production. Based on studies of persistent deep-sea seeps at mid- and northern latitudes, researchers have learned that bacteria and archaea can create a \"sediment filter\" that oxidizes methane prior to its release. Antarctica is thought to contain large reservoirs of organic carbon buried beneath its ice which could a quantity of methane equivalent to all of the permafrost in the Arctic and yet we know almost nothing about the methane oxidizing microbes in this region. How these microbial communities develop and potentially respond to fluctuations in methane levels is an under-explored avenue of research. A bacterial mat was recently discovered at 78 degrees south, suggesting the possible presence of a methane seep, and associated microbial communities. This project will explore this environment in detail to assess the levels and origin of methane, and the nature of the microbial ecosystem present. An expansive bacterial mat appeared and/or was discovered at 78 degrees south in 2011. This site, near McMurdo Station Antarctica, has been visited since the mid-1960s, but this mat was not observed until 2011. The finding of this site provides an unusual opportunity to study an Antarctic marine benthic habitat with active methane cycling and to examine the dynamics of recruitment and community succession of seep fauna including bacteria, archaea, protists and metazoans. This project will collect the necessary baseline data to facilitate further studies of Antarctic methane cycling. The concentration and source of methane will be determined at this site and at potentially analogous sites in McMurdo Sound. In addition to biogeochemical characterization of the sites, molecular analysis of the microbial community will quantify the time scales on which bacteria and archaea respond to methane input and provide information on rates of community development and succession in the Southern Ocean. Project activities will facilitate the training of at least one graduate student and results will be shared at both local and international levels. A female graduate student will be mentored as part of this project and data collected will form part of her dissertation. Lectures will be given in K-12 classrooms in Oregon to excite students about polar science. National and international audiences will be reached through blogs and presentations at a scientific conference. The PI\u0027s previous blogs have been used by K-12 classrooms as part of their lesson plans and followed in over 65 countries.", "east": 166.666, "geometry": "POINT(166.666 -77.8)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "Sea Floor; USAP-DC; Ross Sea; BACTERIA/ARCHAEA; NOT APPLICABLE", "locations": "Ross Sea; Sea Floor", "north": -77.8, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Thurber, Andrew", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "BCO-DMO", "repositories": "BCO-DMO", "science_programs": null, "south": -77.8, "title": "EAGER: Elucidating the Antarctic Methane Cycle at the Cinder Cones Reducing Habitat.", "uid": "p0010030", "west": 166.666}, {"awards": "1543412 Reinfelder, John", "bounds_geometry": null, "dataset_titles": "16S rRNA gene libraries of krill gut microbial communities; Microbial gene libraries of krill gut microbial communities", "datasets": [{"dataset_uid": "200024", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "Microbial gene libraries of krill gut microbial communities", "url": "https://nam02.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fbioproject%2F531145\u0026amp;data=02%7C01%7Creinfeld%40envsci.rutgers.edu%7C7e30a0192dc748ab271408d6b9d57d08%7Cb92d2b234d35447093ff69aca6632ffe%7C1%7C0%7C636900723909188941\u0026amp;sdata=G6cNg4bBHzeikrWSCYITcT6XS3NLWwjQ1yNdwtrALPc%3D\u0026amp;reserved=0"}, {"dataset_uid": "601171", "doi": "10.15784/601171", "keywords": "Antarctica; Biota; Krill; LTER Palmer Station; Microbiome; Oceans; Southern Ocean", "people": "Reinfelder, John", "repository": "USAP-DC", "science_program": "LTER", "title": "16S rRNA gene libraries of krill gut microbial communities", "url": "https://www.usap-dc.org/view/dataset/601171"}], "date_created": "Sun, 31 Mar 2019 00:00:00 GMT", "description": "Marine food webs can concentrate monomethylmercury (MMHg), a neurotoxin in mammals, in upper trophic level consumers. Despite their remoteness, coastal Antarctic marine ecosystems accumulate and biomagnify MMHg to levels observed at lower latitudes and in the Arctic. Marine sediments and other anoxic habitats in the oceans are typical areas where methylation of mercury occurs and these are likely places where MMHg is being produced. Krill, and more specifically their digestive tracts, may be a previously unaccounted for site where the production of MMHg may be occurring in the Antarctic. If monomethylmercury production is occurring in krill, current views regarding bioaccumulation in the food web and processes leading to the production and accumulation of mercury in the Antarctic Ocean could be better informed, if not transformed. This project will conduct a preliminary assessment of the krill gut microbiomes, the microbiome\u0027s genomic content and potential for production of monomethyl mercury by detecting the genes involved in mercury transformations. By analyzing the krill gut microbiome, the project will provide insights regarding animal-microbe interactions and their potential role in globally important biogeochemical cycles. This project will conduct a preliminary assessment of the krill gut microbiomes, the microbiomes genomic content and potential for production of monomethylmercury. The diversity and metabolic profiles of microorganisms in krill digestive tracts will be evaluated using massively parallel Illumina DNA sequencing technology to produce 16S rRNA gene libraries and assembled whole metagenomes. The project will also quantify the abundance and expression of Hg methylation genes, hgcAB, and identify their taxonomic affiliations in the microbiome communities. Environmental metagenomes, 16S rRNA gene inventories produced from this project will provide the polar science community with valuable databases and experimental tools with which to examine coastal Antarctic microbial ecology and biogeochemistry. The project will seek to provide a wider window into the diversity of extremophile microbial communities and the identification of potentially unique and useful bioactive compounds. In addition to public education and outreach. This project will train graduate students and provide educational and outreach opportunities at the participating institutions", "east": -68.2816, "geometry": "POINT(-69.09295 -66.8017)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "USAP-DC; Antarctica; NOT APPLICABLE; BACTERIA/ARCHAEA", "locations": "Antarctica", "north": -65.8708, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Schaefer, Jeffra; Reinfelder, John; Barkar, T.", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "NCBI GenBank", "repositories": "NCBI GenBank; USAP-DC", "science_programs": "LTER", "south": -67.7326, "title": "Methylmercury in Antarctic Krill Microbiomes", "uid": "p0010023", "west": -69.9043}, {"awards": "1144192 Tulaczyk, Slawek; 1144176 Lyons, W. Berry; 1727387 Mikucki, Jill; 1144177 Pettit, Erin", "bounds_geometry": "POLYGON((161.8 -77.7,161.88 -77.7,161.96 -77.7,162.04000000000002 -77.7,162.12 -77.7,162.2 -77.7,162.28 -77.7,162.36 -77.7,162.44 -77.7,162.51999999999998 -77.7,162.6 -77.7,162.6 -77.70700000000001,162.6 -77.714,162.6 -77.721,162.6 -77.728,162.6 -77.735,162.6 -77.742,162.6 -77.749,162.6 -77.756,162.6 -77.76299999999999,162.6 -77.77,162.51999999999998 -77.77,162.44 -77.77,162.36 -77.77,162.28 -77.77,162.2 -77.77,162.12 -77.77,162.04000000000002 -77.77,161.96 -77.77,161.88 -77.77,161.8 -77.77,161.8 -77.76299999999999,161.8 -77.756,161.8 -77.749,161.8 -77.742,161.8 -77.735,161.8 -77.728,161.8 -77.721,161.8 -77.714,161.8 -77.70700000000001,161.8 -77.7))", "dataset_titles": "Ablation Stake Data from of Taylor Glacier near Blood Falls; Antarctica Support 2014/2015 - C-528 Blood Falls GPS/GNSS Observations Dataset; Blood Falls, McMurdo Dry Va. International Federation of Digital Seismograph Networks. Dataset/Seismic Network; FLIR thermal imaging data near Blood Falls, Taylor Glacier; Ground Penetrating Radar Data near Blood Falls, Taylor Glacier; Ice Temperature in Shallow Boreholes Near Blood Falls at the Terminus of Taylor Glacier, McMurdo Dry Valleys, Antarctica; NCBI short read archive -Metagenomic survey of Antarctic Groundwater; Terrestrial Radar Interferometry near Blood Falls, Taylor Glacier; The Geochemistry of englacial brine from Taylor Glacier, Antarctica; Time Lapse imagery of the Blood Falls feature, Antarctica ; Vaisala Integrated Met Station near Blood Falls, Taylor Glacier", "datasets": [{"dataset_uid": "200074", "doi": "", "keywords": null, "people": null, "repository": "NCBI GenBank", "science_program": null, "title": "NCBI short read archive -Metagenomic survey of Antarctic Groundwater", "url": "https://www.ncbi.nlm.nih.gov/sra/?term=SRR6667787"}, {"dataset_uid": "601139", "doi": "10.15784/601139", "keywords": "Antarctica; Borehole; Borehole Logging; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Ice Temperature; Snow/ice; Snow/Ice; Temperature; Temperature Profiles", "people": "Tulaczyk, Slawek", "repository": "USAP-DC", "science_program": null, "title": "Ice Temperature in Shallow Boreholes Near Blood Falls at the Terminus of Taylor Glacier, McMurdo Dry Valleys, Antarctica", "url": "https://www.usap-dc.org/view/dataset/601139"}, {"dataset_uid": "200028", "doi": "10.7283/FCEN-8050", "keywords": null, "people": null, "repository": "UNAVCO", "science_program": null, "title": "Antarctica Support 2014/2015 - C-528 Blood Falls GPS/GNSS Observations Dataset", "url": "https://www.unavco.org/data/doi/10.7283/fcen-8050"}, {"dataset_uid": "200029", "doi": "10.7914/SN/YW_2013", "keywords": null, "people": null, "repository": "IRIS", "science_program": null, "title": "Blood Falls, McMurdo Dry Va. International Federation of Digital Seismograph Networks. Dataset/Seismic Network", "url": "http://www.fdsn.org/networks/detail/YW_2013/"}, {"dataset_uid": "601179", "doi": "10.15784/601179", "keywords": "Antarctica; Chemistry:ice; Chemistry:Ice; Geochemistry; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Subglacial Brine", "people": "Lyons, W. Berry; Gardner, Christopher B.", "repository": "USAP-DC", "science_program": null, "title": "The Geochemistry of englacial brine from Taylor Glacier, Antarctica", "url": "https://www.usap-dc.org/view/dataset/601179"}, {"dataset_uid": "601169", "doi": "10.15784/601169", "keywords": "Antarctica; Basal Crevassing; Glacier Hydrology; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Infrared Imagery; Photo/video; Photo/Video; Taylor Glacier; Thermal Camera; Timelaps Images", "people": "Pettit, Erin", "repository": "USAP-DC", "science_program": null, "title": "FLIR thermal imaging data near Blood Falls, Taylor Glacier", "url": "https://www.usap-dc.org/view/dataset/601169"}, {"dataset_uid": "601167", "doi": "10.15784/601167", "keywords": "Antarctica; Basal Crevassing; Glacier Hydrology; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Photo; Photo/video; Photo/Video; Snow/ice; Snow/Ice; Taylor Glacier; Timelaps Images", "people": "Pettit, Erin", "repository": "USAP-DC", "science_program": null, "title": "Time Lapse imagery of the Blood Falls feature, Antarctica ", "url": "https://www.usap-dc.org/view/dataset/601167"}, {"dataset_uid": "601168", "doi": "10.15784/601168", "keywords": "Antarctica; Atmosphere; Basal Crevassing; Glacier Hydrology; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Meteorology; Taylor Glacier; Temperature; Weather Station Data; Wind Speed", "people": "Pettit, Erin", "repository": "USAP-DC", "science_program": null, "title": "Vaisala Integrated Met Station near Blood Falls, Taylor Glacier", "url": "https://www.usap-dc.org/view/dataset/601168"}, {"dataset_uid": "601166", "doi": "10.15784/601166", "keywords": "Antarctica; Basal Crevassing; Glacier Hydrology; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; GPR; Radar; Snow/ice; Snow/Ice; Taylor Glacier", "people": "Pettit, Erin", "repository": "USAP-DC", "science_program": null, "title": "Terrestrial Radar Interferometry near Blood Falls, Taylor Glacier", "url": "https://www.usap-dc.org/view/dataset/601166"}, {"dataset_uid": "601165", "doi": "10.15784/601165", "keywords": "Antarctica; Basal Crevassing; Glacier Hydrology; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; GPR; Radar; Snow/ice; Snow/Ice; Taylor Glacier", "people": "Pettit, Erin", "repository": "USAP-DC", "science_program": null, "title": "Ground Penetrating Radar Data near Blood Falls, Taylor Glacier", "url": "https://www.usap-dc.org/view/dataset/601165"}, {"dataset_uid": "601164", "doi": "10.15784/601164", "keywords": "Antarctica; Basal Crevassing; Glacier Hydrology; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Snow/ice; Snow/Ice", "people": "Pettit, Erin", "repository": "USAP-DC", "science_program": null, "title": "Ablation Stake Data from of Taylor Glacier near Blood Falls", "url": "https://www.usap-dc.org/view/dataset/601164"}], "date_created": "Wed, 28 Nov 2018 00:00:00 GMT", "description": "Recent discoveries of widespread liquid water and microbial ecosystems below the Antarctic ice sheets have generated considerable interest in studying Antarctic subglacial environments. Understanding subglacial hydrology, the persistence of life in extended isolation and the evolution and stability of subglacial habitats requires an integrated, interdisciplinary approach. The collaborative project, Minimally Invasive Direct Glacial Exploration (MIDGE) of the Biogeochemistry, Hydrology and Glaciology of Blood Falls, McMurdo Dry Valleys will integrate geophysical measurements, molecular microbial ecology and geochemical analyses to explore a unique Antarctic subglacial system known as Blood Falls. Blood Falls is a hypersaline, subglacial brine that supports an active microbial community. The subglacial brine is released from a crevasse at the surface of the Taylor Glacier providing an accessible portal into an Antarctic subglacial ecosystem. Recent geochemical and molecular analyses support a marine source for the salts and microorganisms in Blood Falls. The last time marine waters inundated this part of the McMurdo Dry Valleys was during the Late Tertiary, which suggests the brine is ancient. Still, no direct samples have been collected from the subglacial source to Blood Falls and little is known about the origin of this brine or the amount of time it has been sealed below Taylor Glacier. Radar profiles collected near Blood Falls delineate a possible fault in the subglacial substrate that may help explain the localized and episodic nature of brine release. However it remains unclear what triggers the episodic release of brine exclusively at the Blood Falls crevasse or the extent to which the brine is altered as it makes its way to the surface. The MIDGE project aims to determine the mechanism of brine release at Blood Falls, evaluate changes in the geochemistry and the microbial community within the englacial conduit and assess if Blood Falls waters have a distinct impact on the thermal and stress state of Taylor Glacier, one of the most studied polar glaciers in Antarctica. The geophysical study of the glaciological structure and mechanism of brine release will use GPR, GPS, and a small passive seismic network. Together with international collaborators, the \u0027Ice Mole\u0027 team from FH Aachen University of Applied Sciences, Germany (funded by the German Aerospace Center, DLR), MIDGE will develop and deploy innovative, minimally invasive technologies for clean access and brine sample retrieval from deep within the Blood Falls drainage system. These technologies will allow for the collection of samples of the brine away from the surface (up to tens of meters) for geochemical analyses and microbial structure-function experiments. There is concern over the contamination of pristine subglacial environments from chemical and biological materials inherent in the drilling process; and MIDGE will provide data on the efficacy of thermoelectric probes for clean access and retrieval of representative subglacial samples. Antarctic subglacial environments provide an excellent opportunity for researching survivability and adaptability of microbial life and are potential terrestrial analogues for life habitats on icy planetary bodies. The MIDGE project offers a portable, versatile, clean alternative to hot water and mechanical drilling and will enable the exploration of subglacial hydrology and ecosystem function while making significant progress towards developing technologies for minimally invasive and clean sampling of icy systems.", "east": 162.6, "geometry": "POINT(162.2 -77.735)", "instruments": "NOT APPLICABLE \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "is_usap_dc": true, "keywords": "NOT APPLICABLE; BACTERIA/ARCHAEA; USAP-DC", "locations": null, "north": -77.7, "nsf_funding_programs": "Antarctic Integrated System Science; Antarctic Integrated System Science; Antarctic Integrated System Science; Antarctic Integrated System Science; Antarctic Instrumentation and Support", "paleo_time": null, "persons": "Tulaczyk, Slawek; Pettit, Erin; Lyons, W. Berry; Mikucki, Jill", "platforms": "OTHER \u003e NOT APPLICABLE \u003e NOT APPLICABLE", "repo": "NCBI GenBank", "repositories": "IRIS; NCBI GenBank; UNAVCO; USAP-DC", "science_programs": null, "south": -77.77, "title": "Collaborative Research: MIDGE: Minimally Invasive Direct Glacial Exploration of Biogeochemistry, Hydrology and Glaciology of Blood Falls, McMurdo Dry Valleys", "uid": "p0000002", "west": 161.8}]

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