[{"awards": "1644155 Twining, Benjamin", "bounds_geometry": "POLYGON((78 -68.4,78.05 -68.4,78.1 -68.4,78.15 -68.4,78.2 -68.4,78.25 -68.4,78.3 -68.4,78.35 -68.4,78.4 -68.4,78.45 -68.4,78.5 -68.4,78.5 -68.419,78.5 -68.438,78.5 -68.457,78.5 -68.476,78.5 -68.495,78.5 -68.514,78.5 -68.533,78.5 -68.552,78.5 -68.571,78.5 -68.59,78.45 -68.59,78.4 -68.59,78.35 -68.59,78.3 -68.59,78.25 -68.59,78.2 -68.59,78.15 -68.59,78.1 -68.59,78.05 -68.59,78 -68.59,78 -68.571,78 -68.552,78 -68.533,78 -68.514,78 -68.495,78 -68.476,78 -68.457,78 -68.438,78 -68.419,78 -68.4))", "dataset_titles": "Flow cytometry enumeration of virus-like and bacteria-like abundance in Ace, Deep, \u0026 Organic lakes (Antarctica)", "datasets": [{"dataset_uid": "601626", "doi": "10.15784/601626", "keywords": "Ace Lake; Antarctica; Deep Lake; Organic Lake; Vestfold Hills", "people": "Twining, Benjamin; Martinez-Martinez, Joaquin", "repository": "USAP-DC", "science_program": null, "title": "Flow cytometry enumeration of virus-like and bacteria-like abundance in Ace, Deep, \u0026 Organic lakes (Antarctica)", "url": "https://www.usap-dc.org/view/dataset/601626"}], "date_created": "Fri, 06 Aug 2021 00:00:00 GMT", "description": "Viruses are prevalent in aquatic environments where they reach up to five hundred million virus particles in a teaspoon of water. Ongoing discovery of viruses seems to confirm current understanding that all forms of life can host and be infected by viruses and that viruses are one of the largest reservoirs of unexplored genetic diversity on Earth. This study aims to better understand interactions between specific viruses and phytoplankton hosts and determine how these viruses may affect different algal groups present within lakes of the Vestfold Hills, Antarctica. These lakes (Ace, Organic and Deep)were originally derived from the ocean and contain a broad range of saline conditions with a similarly broad range of physicochemical characteristics resulting from isolation and low external influence for thousands of years. These natural laboratories allow examination of microbial processes and interactions that would be difficult to characterize elsewhere on earth. The project will generate extensive genomic information that will be made freely available. The project will also leverage the study of viruses and the genomic approaches employed to advance the training of undergraduate students and to engage and foster an understanding of Antarctic science and studies of microbes during a structured informal education program in Maine for the benefit of high school students. By establishing the dynamics and interactions of (primarily) specific dsDNA virus groups in different habitats with different redox conditions throughout seasonal and inter annual cycles the project will learn about the biotic and abiotic factors that influence microbial community dynamics. This project does not require fieldwork in Antarctica. Instead, the investigators will leverage already collected and archived samples from three lakes that have concurrent measures of physicochemical information. Approximately 2 terabyte of Next Generation Sequencing (NGS) (including metagenomes, SSU rRNA amplicons and single virus genomes) will be generated from selected available samples through a Community Science Program (CSP) funded by the Joint Genome Institute. The investigators will employ bioinformatics to interrogate those sequence databases. In particular, they will focus on investigating the presence, phylogeny and co-occurrence of polintons, polinton-like viruses, virophages and large dsDNA phytoplankton viruses as well as of their putative eukaryotic microbial hosts. Bioinformatic analyses will be complemented with quantitative digital PCR and microbial association network analysis to detect specific virus?virus?host interactions from co-occurrence spatial and temporal patterns. Multivariate analysis and network analyses will also be performed to investigate which abiotic factors most closely correlate with phytoplankton and virus abundances, temporal dynamics, and observed virus-phytoplankton associations within the three lakes. The results of this project will improve understanding of phytoplankton and their viruses as vital components of the carbon cycle in Antarctic, marine-derived aquatic environments, and likely in any other aquatic environment. Overall, this work will advance understanding of the genetic underpinnings of adaptations in unique Antarctic environments.", "east": 78.5, "geometry": "POINT(78.25 -68.495)", "instruments": null, "is_usap_dc": true, "keywords": "LABORATORY; AMD; USAP-DC; VIRUSES; Vestfold Hills; Amd/Us; FIELD SURVEYS; USA/NSF", "locations": "Vestfold Hills", "north": -68.4, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Twining, Benjamin; Martinez-Martinez, Joaquin", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS; OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -68.59, "title": "Viral control of microbial communities in Antarctic lakes", "uid": "p0010237", "west": 78.0}]

Help on the Results MapX

This window can be dragged by its header, and can be resized from the bottom right corner.

The Results Map and the Results Table

The Results Map displays the centroids of the geographic bounds of all the results returned by the search.
Results that are displayed in the current map view will be highlighted in blue and brought to the top of the Results Table.
As the map is panned or zoomed, the highlighted rows in the table will update.
If you click on a centroid on the map, it will turn yellow and display a popup with details for that project/dataset - including a link to the landing page. The bounds for the project(s)/dataset(s) selected will be displayed in red. The selected result(s) will be highlighted in red and brought to the top of the table.
The default table sorting order is: Selected, Visible, Date (descending), but this can be changed by clicking on column headers in the table.
Selecting Show on Map for an individual row will both display the geographic bounds for that result on a mini map, and also display the bounds and highlight the centroid on the Results Map.
Clicking the 'Show boundaries' checkbox at the top of the Results Map will display all the bounds for the filtered results.

Defining a search area on the Results Map

If you click on the Rectangle or Polygon icons in the top right of the Results Map, you can define a search area which will be added to any other search criteria already selected.
After you have drawn a polygon, you can edit it using the Edit Geometry dropdown in the search form at the top.
Clicking Clear in the map will clear any drawn polygon.
Clicking Search in the map, or Search on the form will have the same effect.
The returned results will be any projects/datasets with bounds that intersect the polygon.
Use the Exclude project/datasets checkbox to exclude any projects/datasets that cover the whole Antarctic region.

Viewing map layers on the Results Map

Clicking the Layers button - the blue square in the top left of the Results Map - will display a list of map layers you can add or remove from the currently displayed map view.

Search USAP Projects And Datasets

Free Text Search

Repository

Title (free text)

Science Program

Person

Award

NSF Funding Program

Spatial Bounds of Data Use the Results Map below to draw a polygon over the required search area, or edit the WKT geometry here:

Any WKT polygon, with longitudes in a 0-360 ° range. Example: Ross Sea is between 160 and 210. Read about the WKT format here.

Exclude projects that cover the whole Antarctic from map-based search

1 record(s) found

Older retrieved projects from AMD. Warning: many have incomplete information.

To sort the table of search results, click the header of the column you wish to search by. To sort by multiple columns, hold down the shift key whilst selecting the sort columns in order.

Project Title/Abstract/Map

NSF Award(s)

Date Created

PIs / Scientists

Dataset Links and Repositories

Viral control of microbial communities in Antarctic lakes

1644155

2021-08-06

Twining, Benjamin; Martinez-Martinez, Joaquin

Flow cytometry enumeration of virus-like and bacteria-like abundance in Ace, Deep, & Organic lakes (Antarctica)

USAP-DC

Viruses are prevalent in aquatic environments where they reach up to five hundred million virus particles in a teaspoon of water. Ongoing discovery of viruses seems to confirm current understanding that all forms of life can host and be infected by viruses and that viruses are one of the largest reservoirs of unexplored genetic diversity on Earth. This study aims to better understand interactions between specific viruses and phytoplankton hosts and determine how these viruses may affect different algal groups present within lakes of the Vestfold Hills, Antarctica. These lakes (Ace, Organic and Deep)were originally derived from the ocean and contain a broad range of saline conditions with a similarly broad range of physicochemical characteristics resulting from isolation and low external influence for thousands of years. These natural laboratories allow examination of microbial processes and interactions that would be difficult to characterize elsewhere on earth. The project will generate extensive genomic information that will be made freely available. The project will also leverage the study of viruses and the genomic approaches employed to advance the training of undergraduate students and to engage and foster an understanding of Antarctic science and studies of microbes during a structured informal education program in Maine for the benefit of high school students. By establishing the dynamics and interactions of (primarily) specific dsDNA virus groups in different habitats with different redox conditions throughout seasonal and inter annual cycles the project will learn about the biotic and abiotic factors that influence microbial community dynamics. This project does not require fieldwork in Antarctica. Instead, the investigators will leverage already collected and archived samples from three lakes that have concurrent measures of physicochemical information. Approximately 2 terabyte of Next Generation Sequencing (NGS) (including metagenomes, SSU rRNA amplicons and single virus genomes) will be generated from selected available samples through a Community Science Program (CSP) funded by the Joint Genome Institute. The investigators will employ bioinformatics to interrogate those sequence databases. In particular, they will focus on investigating the presence, phylogeny and co-occurrence of polintons, polinton-like viruses, virophages and large dsDNA phytoplankton viruses as well as of their putative eukaryotic microbial hosts. Bioinformatic analyses will be complemented with quantitative digital PCR and microbial association network analysis to detect specific virus?virus?host interactions from co-occurrence spatial and temporal patterns. Multivariate analysis and network analyses will also be performed to investigate which abiotic factors most closely correlate with phytoplankton and virus abundances, temporal dynamics, and observed virus-phytoplankton associations within the three lakes. The results of this project will improve understanding of phytoplankton and their viruses as vital components of the carbon cycle in Antarctic, marine-derived aquatic environments, and likely in any other aquatic environment. Overall, this work will advance understanding of the genetic underpinnings of adaptations in unique Antarctic environments.