{"dp_type": "Project", "free_text": "Microbial activity"}
[{"awards": "2325922 Couradeau, Estelle", "bounds_geometry": "POLYGON((-73.783 4.679,-73.7827 4.679,-73.7824 4.679,-73.7821 4.679,-73.7818 4.679,-73.7815 4.679,-73.7812 4.679,-73.7809 4.679,-73.7806 4.679,-73.7803 4.679,-73.78 4.679,-73.78 4.6789,-73.78 4.6788,-73.78 4.6787,-73.78 4.6786,-73.78 4.6785,-73.78 4.6784,-73.78 4.6783,-73.78 4.6782,-73.78 4.6781,-73.78 4.678,-73.7803 4.678,-73.7806 4.678,-73.7809 4.678,-73.7812 4.678,-73.7815 4.678,-73.7818 4.678,-73.7821 4.678,-73.7824 4.678,-73.7827 4.678,-73.783 4.678,-73.783 4.6781,-73.783 4.6782,-73.783 4.6783,-73.783 4.6784,-73.783 4.6785,-73.783 4.6786,-73.783 4.6787,-73.783 4.6788,-73.783 4.6789,-73.783 4.679))", "dataset_titles": null, "datasets": null, "date_created": "Tue, 06 Feb 2024 00:00:00 GMT", "description": "As climate change progresses, it is activating both master switches of microbial activity simultaneously: moisture and temperature. In soils, microbes serve critical ecosystem services including nutrient cycling and carbon sequestration. The fact that we do not fully understand how microbes act on carbon pools in soils and how these will change with the rapidly changing climate is extremely worrisome. Here we propose to tackle this question by initiating an interdisciplinary action studying the soils of the Colombian P\u00e1ramos. The P\u00e1ramos are a unique high-altitude hotspot of biodiversity in the Andes that render critical ecosystem services, including water capture and carbon sequestration. If the hydrology of the system has gained a lot of attention over the past few years, the fate of the extensive pools of organic carbon in these vast peatlands has remained unexplored. The unique geographic situation of the cold and humid P\u00e1ramos being already on the edge of their geo-climatic range provides us with an unparalleled opportunity to study a soil microbial community from carbon-rich soils that are extremely vulnerable to both the raise in temperature and decrease of moisture that will impact soils around the globe as climate change progresses. Our project aims at starting to address the need to disentangle the effect of moisture and temperature on the activity and composition of the microbial communities controlling the fate of organic carbon in soils (Objective 1), while assembling an international team of experts capable of scaling up the understanding of these processes at the landscape and regional level by integrating the functioning of the belowground microbial community with the above-ground vegetation (Objective 2).", "east": -73.78, "geometry": "POINT(-73.7815 4.6785)", "instruments": null, "is_usap_dc": true, "keywords": "TERRESTRIAL ECOSYSTEMS; Chingaza Paramos Colombia; ORGANIC MATTER; SOIL MECHANICS", "locations": "Chingaza Paramos Colombia", "north": 4.679, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Couradeau, Estelle; Maximova, Siela; Machado, Jose Luis", "platforms": null, "repositories": null, "science_programs": null, "south": 4.678, "title": "Collaborative Research: BoCP-Design: Climate change alteration of soils functional biodiversity of the P\u00e1ramos, Colombia", "uid": "p0010445", "west": -73.783}, {"awards": "2037963 Smith, Heidi", "bounds_geometry": null, "dataset_titles": null, "datasets": null, "date_created": "Tue, 11 Oct 2022 00:00:00 GMT", "description": "Glacial ice cores serve as a museum back in time, providing detailed records of past climatic conditions. In addition to chronological records such as temperature, chemistry and gas composition, ice provides a unique environment for preserving microbes and other biological materials through time. These microbes provide invaluable insight into the physiological capabilities necessary for survival in the Earths cryosphere and other icy planetary bodies, yet little is known about them. This award supports fundamental research into the activity of microbes in ice, and directly supports major research priorities regarding Antarctic biota identified in the 2015 National Academies of Sciences, Engineering, and Medicine report, A Strategic Vision for NSF Investments in Antarctic and Southern Ocean Research. The broader impacts of this work are that it will be relevant to researchers across paleoclimate and biological fields. It will support two early career researchers, a graduate and an undergraduate student who will conduct laboratory analyses, participate in outreach activities, publish papers in scientific journals and present at conferences. \r\n\r\nThis work will use previously collected ice cores to investigate englacial microbial activity from the Holocene back to the Last Glacial Maximum from the blue ice area of Taylor Glacier, Antarctica. The proposal identified making significant contributions to 1) investing how Antarctic organisms evolve and adapt to changing environment, 2) understanding how microbes alter the preservation of paleorecord-relevant gas and trace element information in ice cores, and 3) identifying microbial life in cores and their activity in relation to dust depositional events. Two recently developed complementary techniques (bio-orthogonal noncanonical amino acid tagging and deuterium isotope probing) in combination with Raman Confocal Microspectroscopy will be used to assess and quantify microbial activity in ice. During phase one of the project, these methods will be optimized using deaccessioned ice cores available at the National Science Foundations Ice Core Facility. In phase two, ice cores in a time series from the Taylor Glacier will be analyzed for geochemistry and microbial activity. Research results will provide a comprehensive view of englacial microbial communities, including their metabolic diversity and activity, and the effect of geochemical parameters on microbial assemblages from different climate periods. Given the dearth of information available on englacial microbial communities, the results of this research will be of particular significance.\r\n\r\nThis award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": null, "geometry": null, "instruments": null, "is_usap_dc": true, "keywords": "Microbial Activity; LABORATORY; Paleoclimate; CAMP; Taylor Glacier; Microbiology; Alaska; ICE CORE RECORDS", "locations": "CAMP; Alaska; Taylor Glacier", "north": null, "nsf_funding_programs": "Antarctic Integrated System Science", "paleo_time": null, "persons": "Smith, Heidi; Foreman, Christine; Dieser, Markus", "platforms": "OTHER \u003e PHYSICAL MODELS \u003e LABORATORY", "repositories": null, "science_programs": null, "south": null, "title": "Life in Ice: Probing Microbial Englacial Activity through Time", "uid": "p0010385", "west": null}, {"awards": "2147045 Learman, Deric", "bounds_geometry": "POLYGON((-180 -60,-168 -60,-156 -60,-144 -60,-132 -60,-120 -60,-108 -60,-96 -60,-84 -60,-72 -60,-60 -60,-60 -62,-60 -64,-60 -66,-60 -68,-60 -70,-60 -72,-60 -74,-60 -76,-60 -78,-60 -80,-72 -80,-84 -80,-96 -80,-108 -80,-120 -80,-132 -80,-144 -80,-156 -80,-168 -80,180 -80,171 -80,162 -80,153 -80,144 -80,135 -80,126 -80,117 -80,108 -80,99 -80,90 -80,90 -78,90 -76,90 -74,90 -72,90 -70,90 -68,90 -66,90 -64,90 -62,90 -60,99 -60,108 -60,117 -60,126 -60,135 -60,144 -60,153 -60,162 -60,171 -60,-180 -60))", "dataset_titles": null, "datasets": null, "date_created": "Tue, 30 Aug 2022 00:00:00 GMT", "description": "Microbes in Antarctic surface marine sediments have an important role in degrading organic matter and releasing nutrients to the ocean. Organic matter degradation is at the center of the carbon cycle in the ocean, providing valuable information on nutrient recycling, food availability to animals and carbon dioxide release to the atmosphere. The functionality of these microbes has been inferred by their genomics, however these methods only address the possible function, not their actual rates. In this project the PIs plan to combine genomics methods with cellular estimates of enzyme abundance and activity as a way to determine the rates of carbon degradation. This project aims to sample in several regions of Antarctica to provide a large-scale picture of the processes under study and understand the importance of microbial community composition and environmental factors, such as primary productivity, have on microbial activity. The proposed work will combine research tools such as metagenomics, meta-transcriptomics, and metabolomics coupled with chemical data and enzyme assays to establish degradation of organic matter in Antarctic sediments. This project benefits NSFs goals of understanding the adaptation of Antarctic organisms to the cold and isolated environment, critical to predict effects of climate change to polar organisms, as well as contribute to our knowledge of how Antarctic organisms have adapted to this environment. Society will benefit from this project by education of 2 graduate students, undergraduates and K-12 students as well as increase public literacy through short videos production shared in YouTube.\r\n\r\nThe PIs propose to advance understanding of polar microbial community function, by measuring enzyme and gene function of complex organic matter degradation in several ocean regions, providing a circum-Antarctic description of sediment processes. Two hypotheses are proposed. The first hypothesis states that many genes for the degradation of complex organic matter will be shared in sediments throughout a sampling transect and that where variations in gene content occur, it will reflect differences in the quantity and quality of organic matter, not regional variability. The second hypothesis states that a fraction of gene transcripts for organic matter degradation will not result in measurable enzyme activity due to post-translational modification or rapid degradation of the enzymes. The PIs will analyze sediment cores already collected in a 2020 cruise to the western Antarctic Peninsula with the additional request of participating in a cruise in 2023 to East Antarctica. The PIs will analyze sediments for metagenomics, meta-transcriptomics, and metabolomics coupled with geochemical data and enzyme assays to establish microbial degradation of complex organic matter in Antarctic sediments. Organic carbon concentrations and content in sediments will be measured with \u03b413C, \u03b415N, TOC porewater fluorescence in bulk organic carbon. Combined with determination of geographical variability as well as dependence on carbon sources, results from this study could provide the basis for new hypotheses on how climate variability, with increased water temperature, affects geochemistry in the Southern Ocean.", "east": 90.0, "geometry": "POINT(-165 -70)", "instruments": null, "is_usap_dc": true, "keywords": "BENTHIC; ECOSYSTEM FUNCTIONS; Weddell Sea; Antarctic Peninsula; SEDIMENT CHEMISTRY; R/V NBP", "locations": "Antarctic Peninsula; Weddell Sea", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Learman, Deric", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repositories": null, "science_programs": null, "south": -80.0, "title": "Collaborative Research: ANT LIA: Connecting Metagenome Potential to Microbial Function: Investigating Microbial Degradation of Complex Organic Matter Antarctic Benthic Sediments", "uid": "p0010373", "west": -60.0}, {"awards": "9910098 Fritsen, Christian", "bounds_geometry": null, "dataset_titles": "Expedition Data; Expedition data of LMG0106; Expedition data of LMG0205; Expedition data of NBP0104; Expedition data of NBP0204", "datasets": [{"dataset_uid": "002695", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0106", "url": "https://www.rvdata.us/search/cruise/LMG0106"}, {"dataset_uid": "001757", "doi": "", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data", "url": "https://www.rvdata.us/search/cruise/LMG0205"}, {"dataset_uid": "002643", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of NBP0204", "url": "https://www.rvdata.us/search/cruise/NBP0204"}, {"dataset_uid": "002702", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of LMG0205", "url": "https://www.rvdata.us/search/cruise/LMG0205"}, {"dataset_uid": "002657", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition data of NBP0104", "url": "https://www.rvdata.us/search/cruise/NBP0104"}], "date_created": "Tue, 04 May 2010 00:00:00 GMT", "description": "The U.S. Global Ocean Ecosystems Dynamics (U.S. GLOBEC) program has the goal of understanding and ultimately predicting how populations of marine animal species respond to natural and anthropogenic changes in climate. Research in the Southern Ocean (SO) indicates strong coupling between climatic processes and ecosystem dynamics via the annual formation and destruction of sea ice. The Southern Ocean GLOBEC Program (SO GLOBEC) will investigate the dynamic relationship between physical processes and ecosystem responses through identification of critical parameters that affect the distribution, abundance and population dynamics of target species. The overall goals of the SO GLOBEC program are to elucidate shelf circulation processes and their effect on sea ice formation and krill distribution, and to examine the factors which govern krill survivorship and availability to higher trophic levels, including penguins, seals and whales. The focus of the U.S. contribution to the international SO GLOBEC program will be on winter processes. This component will focus on the distribution and activities of sea ice microbial communities. This will be accomplished using an integrated combination of sampling (vertical profiles, horizontal surveys, and under-ice surveys) and observational protocols. Experiments will be designed to estimate microbial activity within the sea ice and at the ice-seawater interface. The research will be coordinated with components studying the water column productivity and the sea ice habitat. The result of the integrated SO GLOBEC program will be to improve the predictability of living marine resources, especially with respect to local and global climatic shifts.", "east": null, "geometry": null, "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e MAGNETIC/MOTION SENSORS \u003e GRAVIMETERS \u003e GRAVIMETERS; IN SITU/LABORATORY INSTRUMENTS \u003e PROFILERS/SOUNDERS \u003e ACOUSTIC SOUNDERS \u003e MSBS", "is_usap_dc": false, "keywords": "R/V LMG; R/V NBP", "locations": null, "north": null, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Fritsen, Christian; Costa, Daniel", "platforms": "WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V LMG; WATER-BASED PLATFORMS \u003e VESSELS \u003e SURFACE \u003e R/V NBP", "repo": "R2R", "repositories": "R2R", "science_programs": null, "south": null, "title": "GLOBEC: Sea Ice Microbial Communities", "uid": "p0000834", "west": null}, {"awards": "0338267 Gooseff, Michael", "bounds_geometry": "POLYGON((161.6 -77.4,161.773 -77.4,161.946 -77.4,162.119 -77.4,162.292 -77.4,162.465 -77.4,162.638 -77.4,162.811 -77.4,162.984 -77.4,163.157 -77.4,163.33 -77.4,163.33 -77.435,163.33 -77.47,163.33 -77.505,163.33 -77.54,163.33 -77.575,163.33 -77.61,163.33 -77.645,163.33 -77.68,163.33 -77.715,163.33 -77.75,163.157 -77.75,162.984 -77.75,162.811 -77.75,162.638 -77.75,162.465 -77.75,162.292 -77.75,162.119 -77.75,161.946 -77.75,161.773 -77.75,161.6 -77.75,161.6 -77.715,161.6 -77.68,161.6 -77.645,161.6 -77.61,161.6 -77.575,161.6 -77.54,161.6 -77.505,161.6 -77.47,161.6 -77.435,161.6 -77.4))", "dataset_titles": "Antarctic Hydrologic Margin Microbiology and Biogeochemistry - data; Hydrologic Margins Research Project, 2004-2008, McMurdo Dry Valleys", "datasets": [{"dataset_uid": "600016", "doi": "", "keywords": null, "people": "Gooseff, Michael N.", "repository": "USAP-DC", "science_program": null, "title": "Hydrologic Margins Research Project, 2004-2008, McMurdo Dry Valleys", "url": "https://www.usap-dc.org/view/dataset/600016"}, {"dataset_uid": "000238", "doi": "", "keywords": null, "people": null, "repository": "PI website", "science_program": null, "title": "Antarctic Hydrologic Margin Microbiology and Biogeochemistry - data", "url": "http://water.engr.psu.edu/gooseff/web_antarctica/data.html"}], "date_created": "Thu, 11 Sep 2008 00:00:00 GMT", "description": "Aquatic-terrestrial transition zones are crucial environments in understanding the biogeochemistry of landscapes. In temperate watersheds, these areas are generally dominated by riparian zones, which have been identified as regions of special interest for biogeochemistry because of the increased microbial activity in these locations, and because of the importance of these hydrological margins in facilitating and buffering hydrologic and biogeochemical exchanges between terrestrial and aquatic ecosystems. In the Antarctic Dry Valleys, terrestrial-aquatic transition zones are intriguing landscape features because of the vast importance of water in this polar desert, and because the material and energy budgets of dry valley ecosystems are linked by hydrology. Hydrological margins in aquatic-terrestrial transition zones will be studied in the Dry Valleys of Antarctica to answer two overarching questions: (1) what are the major controls over hydrologic and biogeochemical exchange across aquaticterrestrial transition zones and (2) to what extent do trends in nutrient cycling (e.g. nitrogen cycling) across these transition zones reflect differences in microbial communities or function vs. differences in the physical and chemical environment (e.g., redox potential)? The hydrologic gradients that define these interfaces provide the opportunity to assess the relative influence of physical conditions and microbial biodiversity and functioning upon biogeochemical cycling. Coordinated hydrologic, biogeochemical, and molecular microbial studies will be executed within hydrologic margins with the following research objectives: to determine the role of sediment characteristics, permafrost and active layer dynamics, and topography on sub-surface water content and distribution in hydrologic margins, to determine the extent to which transformations of nitrogen in hydrological margins are influenced by physical conditions (i.e., moisture, redox potential and pH) or by the presence of specific microbial communities (e.g., denitrifiers), and to characterize the microbial community structure and function of saturated zones.\u003cbr/\u003e\u003cbr/\u003eThis proposed research will provide an improved understanding of the interaction of liquid water, soils, microbial communities, and biogeochemistry within the important hydrologic margin landscape units of the dry valleys. Dry valleys streams and lakes are unique because there is no influence of higher vegetation on the movement of water and may therefore provide a model system for understanding physical and hydrological influences on microbial ecology and biogeochemistry. Hence the findings will contribute to Antarctic science as well as the broader study of riparian zones and hydrologic margins worldwide. Graduate students and undergraduate students will be involved with fieldwork and research projects. Information will be disseminated through a project web site, and outreach activities will include science education in local elementary, middle and high schools near the three universities involved.", "east": 163.33, "geometry": "POINT(162.465 -77.575)", "instruments": null, "is_usap_dc": false, "keywords": "Not provided", "locations": null, "north": -77.4, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Gooseff, Michael N.; Barrett, John; Takacs-Vesbach, Cristina", "platforms": "Not provided", "repo": "USAP-DC", "repositories": "PI website; USAP-DC", "science_programs": null, "south": -77.75, "title": "Collaborative Research: Hydrologic Controls over Biogeochemistry and Microbial Community Structure and Function across Terrestrial/Aquatic Interfaces in a Polar Desert", "uid": "p0000340", "west": 161.6}]
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Project Title/Abstract/Map | NSF Award(s) | Date Created | PIs / Scientists | Dataset Links and Repositories | Abstract | Bounds Geometry | Geometry | Selected | Visible | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Collaborative Research: BoCP-Design: Climate change alteration of soils functional biodiversity of the Páramos, Colombia
|
2325922 |
2024-02-06 | Couradeau, Estelle; Maximova, Siela; Machado, Jose Luis | No dataset link provided | As climate change progresses, it is activating both master switches of microbial activity simultaneously: moisture and temperature. In soils, microbes serve critical ecosystem services including nutrient cycling and carbon sequestration. The fact that we do not fully understand how microbes act on carbon pools in soils and how these will change with the rapidly changing climate is extremely worrisome. Here we propose to tackle this question by initiating an interdisciplinary action studying the soils of the Colombian Páramos. The Páramos are a unique high-altitude hotspot of biodiversity in the Andes that render critical ecosystem services, including water capture and carbon sequestration. If the hydrology of the system has gained a lot of attention over the past few years, the fate of the extensive pools of organic carbon in these vast peatlands has remained unexplored. The unique geographic situation of the cold and humid Páramos being already on the edge of their geo-climatic range provides us with an unparalleled opportunity to study a soil microbial community from carbon-rich soils that are extremely vulnerable to both the raise in temperature and decrease of moisture that will impact soils around the globe as climate change progresses. Our project aims at starting to address the need to disentangle the effect of moisture and temperature on the activity and composition of the microbial communities controlling the fate of organic carbon in soils (Objective 1), while assembling an international team of experts capable of scaling up the understanding of these processes at the landscape and regional level by integrating the functioning of the belowground microbial community with the above-ground vegetation (Objective 2). | POLYGON((-73.783 4.679,-73.7827 4.679,-73.7824 4.679,-73.7821 4.679,-73.7818 4.679,-73.7815 4.679,-73.7812 4.679,-73.7809 4.679,-73.7806 4.679,-73.7803 4.679,-73.78 4.679,-73.78 4.6789,-73.78 4.6788,-73.78 4.6787,-73.78 4.6786,-73.78 4.6785,-73.78 4.6784,-73.78 4.6783,-73.78 4.6782,-73.78 4.6781,-73.78 4.678,-73.7803 4.678,-73.7806 4.678,-73.7809 4.678,-73.7812 4.678,-73.7815 4.678,-73.7818 4.678,-73.7821 4.678,-73.7824 4.678,-73.7827 4.678,-73.783 4.678,-73.783 4.6781,-73.783 4.6782,-73.783 4.6783,-73.783 4.6784,-73.783 4.6785,-73.783 4.6786,-73.783 4.6787,-73.783 4.6788,-73.783 4.6789,-73.783 4.679)) | POINT(-73.7815 4.6785) | false | false | |||||||||||
Life in Ice: Probing Microbial Englacial Activity through Time
|
2037963 |
2022-10-11 | Smith, Heidi; Foreman, Christine; Dieser, Markus | No dataset link provided | Glacial ice cores serve as a museum back in time, providing detailed records of past climatic conditions. In addition to chronological records such as temperature, chemistry and gas composition, ice provides a unique environment for preserving microbes and other biological materials through time. These microbes provide invaluable insight into the physiological capabilities necessary for survival in the Earths cryosphere and other icy planetary bodies, yet little is known about them. This award supports fundamental research into the activity of microbes in ice, and directly supports major research priorities regarding Antarctic biota identified in the 2015 National Academies of Sciences, Engineering, and Medicine report, A Strategic Vision for NSF Investments in Antarctic and Southern Ocean Research. The broader impacts of this work are that it will be relevant to researchers across paleoclimate and biological fields. It will support two early career researchers, a graduate and an undergraduate student who will conduct laboratory analyses, participate in outreach activities, publish papers in scientific journals and present at conferences. This work will use previously collected ice cores to investigate englacial microbial activity from the Holocene back to the Last Glacial Maximum from the blue ice area of Taylor Glacier, Antarctica. The proposal identified making significant contributions to 1) investing how Antarctic organisms evolve and adapt to changing environment, 2) understanding how microbes alter the preservation of paleorecord-relevant gas and trace element information in ice cores, and 3) identifying microbial life in cores and their activity in relation to dust depositional events. Two recently developed complementary techniques (bio-orthogonal noncanonical amino acid tagging and deuterium isotope probing) in combination with Raman Confocal Microspectroscopy will be used to assess and quantify microbial activity in ice. During phase one of the project, these methods will be optimized using deaccessioned ice cores available at the National Science Foundations Ice Core Facility. In phase two, ice cores in a time series from the Taylor Glacier will be analyzed for geochemistry and microbial activity. Research results will provide a comprehensive view of englacial microbial communities, including their metabolic diversity and activity, and the effect of geochemical parameters on microbial assemblages from different climate periods. Given the dearth of information available on englacial microbial communities, the results of this research will be of particular significance. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria. | None | None | false | false | |||||||||||
Collaborative Research: ANT LIA: Connecting Metagenome Potential to Microbial Function: Investigating Microbial Degradation of Complex Organic Matter Antarctic Benthic Sediments
|
2147045 |
2022-08-30 | Learman, Deric | No dataset link provided | Microbes in Antarctic surface marine sediments have an important role in degrading organic matter and releasing nutrients to the ocean. Organic matter degradation is at the center of the carbon cycle in the ocean, providing valuable information on nutrient recycling, food availability to animals and carbon dioxide release to the atmosphere. The functionality of these microbes has been inferred by their genomics, however these methods only address the possible function, not their actual rates. In this project the PIs plan to combine genomics methods with cellular estimates of enzyme abundance and activity as a way to determine the rates of carbon degradation. This project aims to sample in several regions of Antarctica to provide a large-scale picture of the processes under study and understand the importance of microbial community composition and environmental factors, such as primary productivity, have on microbial activity. The proposed work will combine research tools such as metagenomics, meta-transcriptomics, and metabolomics coupled with chemical data and enzyme assays to establish degradation of organic matter in Antarctic sediments. This project benefits NSFs goals of understanding the adaptation of Antarctic organisms to the cold and isolated environment, critical to predict effects of climate change to polar organisms, as well as contribute to our knowledge of how Antarctic organisms have adapted to this environment. Society will benefit from this project by education of 2 graduate students, undergraduates and K-12 students as well as increase public literacy through short videos production shared in YouTube. The PIs propose to advance understanding of polar microbial community function, by measuring enzyme and gene function of complex organic matter degradation in several ocean regions, providing a circum-Antarctic description of sediment processes. Two hypotheses are proposed. The first hypothesis states that many genes for the degradation of complex organic matter will be shared in sediments throughout a sampling transect and that where variations in gene content occur, it will reflect differences in the quantity and quality of organic matter, not regional variability. The second hypothesis states that a fraction of gene transcripts for organic matter degradation will not result in measurable enzyme activity due to post-translational modification or rapid degradation of the enzymes. The PIs will analyze sediment cores already collected in a 2020 cruise to the western Antarctic Peninsula with the additional request of participating in a cruise in 2023 to East Antarctica. The PIs will analyze sediments for metagenomics, meta-transcriptomics, and metabolomics coupled with geochemical data and enzyme assays to establish microbial degradation of complex organic matter in Antarctic sediments. Organic carbon concentrations and content in sediments will be measured with δ13C, δ15N, TOC porewater fluorescence in bulk organic carbon. Combined with determination of geographical variability as well as dependence on carbon sources, results from this study could provide the basis for new hypotheses on how climate variability, with increased water temperature, affects geochemistry in the Southern Ocean. | POLYGON((-180 -60,-168 -60,-156 -60,-144 -60,-132 -60,-120 -60,-108 -60,-96 -60,-84 -60,-72 -60,-60 -60,-60 -62,-60 -64,-60 -66,-60 -68,-60 -70,-60 -72,-60 -74,-60 -76,-60 -78,-60 -80,-72 -80,-84 -80,-96 -80,-108 -80,-120 -80,-132 -80,-144 -80,-156 -80,-168 -80,180 -80,171 -80,162 -80,153 -80,144 -80,135 -80,126 -80,117 -80,108 -80,99 -80,90 -80,90 -78,90 -76,90 -74,90 -72,90 -70,90 -68,90 -66,90 -64,90 -62,90 -60,99 -60,108 -60,117 -60,126 -60,135 -60,144 -60,153 -60,162 -60,171 -60,-180 -60)) | POINT(-165 -70) | false | false | |||||||||||
GLOBEC: Sea Ice Microbial Communities
|
9910098 |
2010-05-04 | Fritsen, Christian; Costa, Daniel |
|
The U.S. Global Ocean Ecosystems Dynamics (U.S. GLOBEC) program has the goal of understanding and ultimately predicting how populations of marine animal species respond to natural and anthropogenic changes in climate. Research in the Southern Ocean (SO) indicates strong coupling between climatic processes and ecosystem dynamics via the annual formation and destruction of sea ice. The Southern Ocean GLOBEC Program (SO GLOBEC) will investigate the dynamic relationship between physical processes and ecosystem responses through identification of critical parameters that affect the distribution, abundance and population dynamics of target species. The overall goals of the SO GLOBEC program are to elucidate shelf circulation processes and their effect on sea ice formation and krill distribution, and to examine the factors which govern krill survivorship and availability to higher trophic levels, including penguins, seals and whales. The focus of the U.S. contribution to the international SO GLOBEC program will be on winter processes. This component will focus on the distribution and activities of sea ice microbial communities. This will be accomplished using an integrated combination of sampling (vertical profiles, horizontal surveys, and under-ice surveys) and observational protocols. Experiments will be designed to estimate microbial activity within the sea ice and at the ice-seawater interface. The research will be coordinated with components studying the water column productivity and the sea ice habitat. The result of the integrated SO GLOBEC program will be to improve the predictability of living marine resources, especially with respect to local and global climatic shifts. | None | None | false | false | |||||||||||
Collaborative Research: Hydrologic Controls over Biogeochemistry and Microbial Community Structure and Function across Terrestrial/Aquatic Interfaces in a Polar Desert
|
0338267 |
2008-09-11 | Gooseff, Michael N.; Barrett, John; Takacs-Vesbach, Cristina |
|
Aquatic-terrestrial transition zones are crucial environments in understanding the biogeochemistry of landscapes. In temperate watersheds, these areas are generally dominated by riparian zones, which have been identified as regions of special interest for biogeochemistry because of the increased microbial activity in these locations, and because of the importance of these hydrological margins in facilitating and buffering hydrologic and biogeochemical exchanges between terrestrial and aquatic ecosystems. In the Antarctic Dry Valleys, terrestrial-aquatic transition zones are intriguing landscape features because of the vast importance of water in this polar desert, and because the material and energy budgets of dry valley ecosystems are linked by hydrology. Hydrological margins in aquatic-terrestrial transition zones will be studied in the Dry Valleys of Antarctica to answer two overarching questions: (1) what are the major controls over hydrologic and biogeochemical exchange across aquaticterrestrial transition zones and (2) to what extent do trends in nutrient cycling (e.g. nitrogen cycling) across these transition zones reflect differences in microbial communities or function vs. differences in the physical and chemical environment (e.g., redox potential)? The hydrologic gradients that define these interfaces provide the opportunity to assess the relative influence of physical conditions and microbial biodiversity and functioning upon biogeochemical cycling. Coordinated hydrologic, biogeochemical, and molecular microbial studies will be executed within hydrologic margins with the following research objectives: to determine the role of sediment characteristics, permafrost and active layer dynamics, and topography on sub-surface water content and distribution in hydrologic margins, to determine the extent to which transformations of nitrogen in hydrological margins are influenced by physical conditions (i.e., moisture, redox potential and pH) or by the presence of specific microbial communities (e.g., denitrifiers), and to characterize the microbial community structure and function of saturated zones.<br/><br/>This proposed research will provide an improved understanding of the interaction of liquid water, soils, microbial communities, and biogeochemistry within the important hydrologic margin landscape units of the dry valleys. Dry valleys streams and lakes are unique because there is no influence of higher vegetation on the movement of water and may therefore provide a model system for understanding physical and hydrological influences on microbial ecology and biogeochemistry. Hence the findings will contribute to Antarctic science as well as the broader study of riparian zones and hydrologic margins worldwide. Graduate students and undergraduate students will be involved with fieldwork and research projects. Information will be disseminated through a project web site, and outreach activities will include science education in local elementary, middle and high schools near the three universities involved. | POLYGON((161.6 -77.4,161.773 -77.4,161.946 -77.4,162.119 -77.4,162.292 -77.4,162.465 -77.4,162.638 -77.4,162.811 -77.4,162.984 -77.4,163.157 -77.4,163.33 -77.4,163.33 -77.435,163.33 -77.47,163.33 -77.505,163.33 -77.54,163.33 -77.575,163.33 -77.61,163.33 -77.645,163.33 -77.68,163.33 -77.715,163.33 -77.75,163.157 -77.75,162.984 -77.75,162.811 -77.75,162.638 -77.75,162.465 -77.75,162.292 -77.75,162.119 -77.75,161.946 -77.75,161.773 -77.75,161.6 -77.75,161.6 -77.715,161.6 -77.68,161.6 -77.645,161.6 -77.61,161.6 -77.575,161.6 -77.54,161.6 -77.505,161.6 -77.47,161.6 -77.435,161.6 -77.4)) | POINT(162.465 -77.575) | false | false |