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Summary table of conditions tested, replicates of each condition, as well as the number of tracks and frames analyzed for the horizontal flume experiments.
Cite as
Fields, D. (2026) "Summary table of conditions tested, replicates of each condition, as well as the number of tracks and frames analyzed for the horizontal flume experiments." U.S. Antarctic Program (USAP) Data Center. doi: https://doi.org/None.
Abstract
A flume similar to that used in Weissburg et al. (2019) was utilized to examine krill swimming behavior in the presence of Guano and Guano+CHL at several different flow speeds. The flume itself was constructed of stainless steel and plexiglass. Water entered the flume upwelling section by two 5 cm bulkhead fittings, with a downward angled deflector to remove large amplitude fluid motion, and 2.5 mm hexagonal cell fiberglass baffling further conditioned the flow. A stainless-steel contraction section provided a smooth narrowing to a final width of 25 cm in the working section (35 x 25 cm), with an additional 25 cm exit section terminating in a tail gate with a 3-inch bulkhead through with the water flowed into a sump. The upstream and downstream end of the working section was fitted with a stainless-steel mesh (5 mm mesh size), to prevent krill from leaving the working section of the flume. The sump (a 210 L clean hdpe barrel) contained a 1 hp Tsurumi pump that returned water to the upwelling section via 5 cm pvc hose. A ball valve on the pump’s output regulated flow velocity.

Each trial was run with 6 – 8 krill for 5 minutes with a water depth of 18 cm in dim light equivalent [NH1] to light at 40 m depth (See Light Measurements). Three chemical conditions were tested: 3 µg CHL L-1 (Ambient), [NH2] 0.1 ug guano L-1 (Guano) or 3 µg CHL L-1 and 0.1 µg guano L-1 (CHL+Guano). The ambient CHL at Palmer Station varied from 0 – 3.2 µg L-1 from October – December 2022 as measured hourly by a fluorometer in the water wall system of the aquarium. For our Ambient treatment trials, we used the upper end of this range as it induced noticeable feeding behavior in the krill within the aquarium tanks. Behavior trials were conducted at 3 and 5.9 cm s-1 for each level of chemical stimulus treatment. Trials were recorded on 2 perpendicularly mounted cameras (FLIR Flea USB3, Canada) at 30 fps.

Flow rate at different valve settings was measured at least once per day by tracking a small amount of neutrally buoyant dye that was injected gently at the beginning of the working section of the flume, using ImageJ. Dye was injected at mid depth and multiple positions (always at least 2 cm away from the wall). Three to five replicate velocity measurements were taken for flow calibration to compute mean treatment speeds (at least 4 replicate flow calibration trials for each velocity condition). The standard deviations between flow measurements were less than 10% of the calculated mean speed across all conditions.
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Date Created:
2026-06-09
Repository:
USAP-DC (current)
Temporal Extent(s)
Start: 2022-09-03 - End: 2022-12-12
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Version:
1
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