USAP-DC

{"dp_type": "Dataset", "free_text": "Microscopy"}

[{"awards": "1341339 Baker, Bill; 1341333 McClintock, James", "bounds_geometry": ["POLYGON((-64.3622 -64.72805,-64.325087 -64.72805,-64.287974 -64.72805,-64.250861 -64.72805,-64.213748 -64.72805,-64.176635 -64.72805,-64.139522 -64.72805,-64.102409 -64.72805,-64.065296 -64.72805,-64.028183 -64.72805,-63.99107 -64.72805,-63.99107 -64.74691299999999,-63.99107 -64.765776,-63.99107 -64.784639,-63.99107 -64.803502,-63.99107 -64.82236499999999,-63.99107 -64.841228,-63.99107 -64.860091,-63.99107 -64.878954,-63.99107 -64.897817,-63.99107 -64.91668,-64.028183 -64.91668,-64.065296 -64.91668,-64.102409 -64.91668,-64.139522 -64.91668,-64.176635 -64.91668,-64.213748 -64.91668,-64.250861 -64.91668,-64.287974 -64.91668,-64.325087 -64.91668,-64.3622 -64.91668,-64.3622 -64.897817,-64.3622 -64.878954,-64.3622 -64.860091,-64.3622 -64.841228,-64.3622 -64.82236499999999,-64.3622 -64.803502,-64.3622 -64.784639,-64.3622 -64.765776,-64.3622 -64.74691299999999,-64.3622 -64.72805))"], "date_created": "Tue, 22 Nov 2022 00:00:00 GMT", "description": "Data from Sabrina Heiser\u0027s study of Plocamium sp. benthic transect and transplant studies. ", "east": -63.99107, "geometry": ["POINT(-64.176635 -64.82236499999999)"], "keywords": "Antarctica; Benthic; Biota; Macroalgae; Mesograzer; Microscopy; Oceans; Secondary Metabolites", "locations": "Antarctica", "north": -64.72805, "nsf_funding_programs": "Antarctic Organisms and Ecosystems; Antarctic Organisms and Ecosystems", "persons": "Amsler, Charles", "project_titles": "Collaborative Research: The Chemical Ecology of Shallow-water Marine Macroalgae and Invertebrates on the Antarctic Peninsula - continuing", "projects": [{"proj_uid": "p0010016", "repository": "USAP-DC", "title": "Collaborative Research: The Chemical Ecology of Shallow-water Marine Macroalgae and Invertebrates on the Antarctic Peninsula - continuing"}], "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -64.91668, "title": "Plocamium transect and transplant data", "uid": "601621", "west": -64.3622}, {"awards": "9909367 Leventer, Amy", "bounds_geometry": ["POLYGON((57 -66,57.3 -66,57.6 -66,57.9 -66,58.2 -66,58.5 -66,58.8 -66,59.1 -66,59.4 -66,59.7 -66,60 -66,60 -66.1,60 -66.2,60 -66.3,60 -66.4,60 -66.5,60 -66.6,60 -66.7,60 -66.8,60 -66.9,60 -67,59.7 -67,59.4 -67,59.1 -67,58.8 -67,58.5 -67,58.2 -67,57.9 -67,57.6 -67,57.3 -67,57 -67,57 -66.9,57 -66.8,57 -66.7,57 -66.6,57 -66.5,57 -66.4,57 -66.3,57 -66.2,57 -66.1,57 -66))"], "date_created": "Thu, 25 Apr 2019 00:00:00 GMT", "description": "This data set describes diatom assemblages and abundances from two sediment cores retrieved from Edward VIII Gulf. The assemblages are used to reconstruct paleoceanographic conditions throughout the Holocene.", "east": 60.0, "geometry": ["POINT(58.5 -66.5)"], "keywords": "Antarctica; Biota; Diatom; East Antarctica; Microscopy; NBP0101; Oceans; Paleoceanography; Paleoclimate; R/v Nathaniel B. Palmer; Sediment Corer", "locations": "East Antarctica; Antarctica", "north": -66.0, "nsf_funding_programs": "Antarctic Integrated System Science", "persons": "Leventer, Amy", "project_titles": "Quaternary Glacial History and Paleoenvironments of the East Antarctic Margin", "projects": [{"proj_uid": "p0000609", "repository": "USAP-DC", "title": "Quaternary Glacial History and Paleoenvironments of the East Antarctic Margin"}], "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -67.0, "title": "Diatom assemblages from Edward VIII Gulf, Kemp Coast, East Antarctica", "uid": "601177", "west": 57.0}, {"awards": "1341333 McClintock, James", "bounds_geometry": ["POLYGON((-65 -63,-64.8 -63,-64.6 -63,-64.4 -63,-64.2 -63,-64 -63,-63.8 -63,-63.6 -63,-63.4 -63,-63.2 -63,-63 -63,-63 -63.2,-63 -63.4,-63 -63.6,-63 -63.8,-63 -64,-63 -64.2,-63 -64.4,-63 -64.6,-63 -64.8,-63 -65,-63.2 -65,-63.4 -65,-63.6 -65,-63.8 -65,-64 -65,-64.2 -65,-64.4 -65,-64.6 -65,-64.8 -65,-65 -65,-65 -64.8,-65 -64.6,-65 -64.4,-65 -64.2,-65 -64,-65 -63.8,-65 -63.6,-65 -63.4,-65 -63.2,-65 -63))"], "date_created": "Mon, 04 Mar 2019 00:00:00 GMT", "description": "These three spreadsheets contain all the data used in Amsler et al. 2019. Impacts of macroalgal-associated gastropods on epiphytic microalgae on the ecologically important Antarctic brown alga Himantothallus grandifolius. Antarctic Science 31: doi:10.1017/S0954102019000014", "east": -63.0, "geometry": ["POINT(-64 -64)"], "keywords": "Antarctica; Benthic; Biota; Macroalgae; Mesograzer; Microscopy; Oceans; Zooplankton", "locations": "Antarctica", "north": -63.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "persons": "Amsler, Charles", "project_titles": "Collaborative Research: The Chemical Ecology of Shallow-water Marine Macroalgae and Invertebrates on the Antarctic Peninsula - continuing", "projects": [{"proj_uid": "p0010016", "repository": "USAP-DC", "title": "Collaborative Research: The Chemical Ecology of Shallow-water Marine Macroalgae and Invertebrates on the Antarctic Peninsula - continuing"}], "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -65.0, "title": "Data from Amsler et al. 2019 Antarctic Science, doi:10.1017/S0954102019000014", "uid": "601159", "west": -65.0}, {"awards": "1048343 Warny, Sophie", "bounds_geometry": ["POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))"], "date_created": "Fri, 01 Feb 2019 00:00:00 GMT", "description": "Thanks to grant # U.S. National Science Foundation ANT-1048343, our group was selected to study about 700 of the recently-acquired sediment samples in Antarctica, covering ~9 regions and geological time frames ranging from the Paleocene to today. The samples were processed for palynological analyses and the slides are curated at the LSU CENEX center.", "east": 180.0, "geometry": ["POINT(0 -89.999)"], "keywords": "Antarctica; Glaciology; Marine Geoscience; Marine Sediments; Microscope; Microscopy; Paleoclimate; Pollen", "locations": "Antarctica", "north": -60.0, "nsf_funding_programs": "Antarctic Earth Sciences", "persons": "Warny, Sophie", "project_titles": "CAREER: Deciphering Antarctic Climate Variability during the Temperate/Polar Transition and Improving Climate Change Literacy in Louisiana through a Companion Outreach Program", "projects": [{"proj_uid": "p0000311", "repository": "USAP-DC", "title": "CAREER: Deciphering Antarctic Climate Variability during the Temperate/Polar Transition and Improving Climate Change Literacy in Louisiana through a Companion Outreach Program"}], "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -90.0, "title": "Palynological samples", "uid": "601151", "west": -180.0}, {"awards": "1341485 Woods, H. Arthur", "bounds_geometry": ["POLYGON((163.85 -77.6,164.134 -77.6,164.418 -77.6,164.702 -77.6,164.986 -77.6,165.27 -77.6,165.554 -77.6,165.838 -77.6,166.122 -77.6,166.406 -77.6,166.69 -77.6,166.69 -77.624,166.69 -77.648,166.69 -77.672,166.69 -77.696,166.69 -77.72,166.69 -77.744,166.69 -77.768,166.69 -77.792,166.69 -77.816,166.69 -77.84,166.406 -77.84,166.122 -77.84,165.838 -77.84,165.554 -77.84,165.27 -77.84,164.986 -77.84,164.702 -77.84,164.418 -77.84,164.134 -77.84,163.85 -77.84,163.85 -77.816,163.85 -77.792,163.85 -77.768,163.85 -77.744,163.85 -77.72,163.85 -77.696,163.85 -77.672,163.85 -77.648,163.85 -77.624,163.85 -77.6))"], "date_created": "Sat, 22 Dec 2018 00:00:00 GMT", "description": "Raw data from Lane, SJ, AL Moran, CM Shishido, BW Tobalske, HA Woods (2018) Cuticular gas exchange by Antarctic sea spiders. Journal of Experimental Biology. jeb.177568 doi: 10.1242/jeb.177568.\r\n\r\nThe file contains data on pore morphology, cuticle thickness, oxygen gradients across the cuticle, and estimated resistances of the cuticle to oxygen flux. Most of the sea spiders were collected near McMurdo Station, with a few extras collected at New Harbor, Antarctica.", "east": 166.69, "geometry": ["POINT(165.27 -77.72)"], "keywords": "Antarctica; Benthos; Biota; Body Size; Cuticle; Mcmurdo Sound; Microelectrodes; Microscope; Microscopy; Oxygen; Pore; Respiration; Sea Spider; Southern Ocean", "locations": "Mcmurdo Sound; Southern Ocean; Antarctica", "north": -77.6, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "persons": "Woods, H. Arthur; Arthur Woods, H.", "project_titles": "Collaborative Research: Body Size, Oxygen, and Vulnerability to Climate Change in Antarctic Pycnogonida", "projects": [{"proj_uid": "p0000007", "repository": "USAP-DC", "title": "Collaborative Research: Body Size, Oxygen, and Vulnerability to Climate Change in Antarctic Pycnogonida"}], "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -77.84, "title": "Cuticle morphology and oxygen gradients of Antarctic sea spiders", "uid": "601145", "west": 163.85}, {"awards": "1341612 Bowser, Samuel", "bounds_geometry": ["POINT(163.5117 -77.57623)"], "date_created": "Wed, 28 Nov 2018 00:00:00 GMT", "description": "This data set comprises scanning electron micrographs of Astrammina rara exposed to sub-lethal concentrations of lead and cadmium. Specimens were shucked (i.e., shells removed) and incubated with metal-free plastic beads for two weeks. Control (no Pb or Cd) and experimental (1 and 5 ug/ml Pb; 0.5 and 1 ug/ml Cd) specimens were prepared for SEM using fixation and dehydration in ethanol (i.e., no aldehydes) and were imaged uncoated at 3keV.", "east": 163.5117, "geometry": ["POINT(163.5117 -77.57623)"], "keywords": "Antarctica; Biota; Foraminifera; Heavy Metal Toxicity; Scanning Electron Microscop; Scanning Electron Microscope (SEM) Images; Scanning Electron Microscopy; Transantarctic Mountains", "locations": "Transantarctic Mountains; Antarctica", "north": -77.57623, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "persons": "Bowser, Samuel; Andreas, Amanda", "project_titles": "Assembling and Mining the Genomes of Giant Antarctic Foraminifera", "projects": [{"proj_uid": "p0000004", "repository": "USAP-DC", "title": "Assembling and Mining the Genomes of Giant Antarctic Foraminifera"}], "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -77.57623, "title": "Scanning electron micrographs: Influence of heavy metal (Pb, Cd) exposure on shell morphogenesis in Astrammina rara, a giant agglutinated Antarctic foraminiferan protist.", "uid": "601138", "west": 163.5117}, {"awards": "1142097 Bochdansky, Alexander", "bounds_geometry": ["POLYGON((163.90952 -67.00006,170.04898 -67.00006,176.18844 -67.00006,182.3279 -67.00006,188.46736 -67.00006,194.60682 -67.00006,200.74628 -67.00006,206.88574 -67.00006,213.0252 -67.00006,219.16466 -67.00006,225.30412 -67.00006,225.30412 -68.15911,225.30412 -69.31816,225.30412 -70.47721,225.30412 -71.63626,225.30412 -72.79531,225.30412 -73.95436,225.30412 -75.11341,225.30412 -76.27246,225.30412 -77.43151,225.30412 -78.59056,219.16466 -78.59056,213.0252 -78.59056,206.88574 -78.59056,200.74628 -78.59056,194.60682 -78.59056,188.46736 -78.59056,182.3279 -78.59056,176.18844 -78.59056,170.04898 -78.59056,163.90952 -78.59056,163.90952 -77.43151,163.90952 -76.27246,163.90952 -75.11341,163.90952 -73.95436,163.90952 -72.79531,163.90952 -71.63626,163.90952 -70.47721,163.90952 -69.31816,163.90952 -68.15911,163.90952 -67.00006))"], "date_created": "Mon, 23 Jan 2017 00:00:00 GMT", "description": null, "east": 225.30412, "geometry": ["POINT(-165.39318 -72.79531)"], "keywords": "Antarctica; Biota; Holographic Microscopy; Oceans; Photo/video; Photo/Video; Phytoplankton; Ross Sea; Sample/collection Description; Sample/Collection Description; Southern Ocean; Video Particle Profiler", "locations": "Ross Sea; Southern Ocean; Antarctica", "north": -67.00006, "nsf_funding_programs": "Antarctic Ocean and Atmospheric Sciences", "persons": "Bochdansky, Alexander", "project_titles": "Collaborative research: TRacing the fate of Algal Carbon Export in the Ross Sea (TRACERS)", "projects": [{"proj_uid": "p0000307", "repository": "USAP-DC", "title": "Collaborative research: TRacing the fate of Algal Carbon Export in the Ross Sea (TRACERS)"}], "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -78.59056, "title": "Video Particle Profiler (VPP) and Digital Inline Holographic Microscopy (DIHM) data from cruise NBP1302", "uid": "600388", "west": 163.90952}, {"awards": "1043690 Scherer, Reed", "bounds_geometry": ["POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))"], "date_created": "Wed, 01 Jan 2014 00:00:00 GMT", "description": "Intellectual Merit: Diatom abundance in sediment cores is typically used as a proxy for paleo primary productivity. This record is complicated by variable preservation, with most loss occurring in the water column via dissolution and zooplankton grazing. This study will investigate preservational biases via a series of controlled experiments to create proxies of original productivity based on morphological changes associated with diatom dissolution and fracture. The PIs will utilize fresh diatoms from culture. Specific objectives include: (1) Linking changes in diatom morphology to availability of dissolved silica and other physical and chemical parameters; (2) Documenting the dissolution process under controlled conditions; (3) Assessment of changes in morphology and diatom surface roughness with increased dissolution; (4) Documenting the physical effects of grazing and fecal pellet formation on diatom fragmentation and dissolution; and (5) Analyzing the impact of diatom dissolution on silica and carbon export. These objectives will be achieved by growing Southern Ocean diatom species in the laboratory under differing physical and chemical conditions; controlled serial dissolution experiments on cultured diatoms; analysis of the dissolution process by imaging frustules under scanning electron microscopy (SEM) and with micro-analysis of surface texture by atomic force microscopy (AFM); making the cultures available to krill and other live zooplankton crustaceans in order to analyze the specific effects of grazing and pelletization on diatom morphology; and comparing experimental results with natural plankton, sediment trap material, and selected Holocene, Pleistocene and Pliocene sediment core material.\nBroader impacts: This work will contribute to understanding of the use of diatom abundance as an indicator of paleoproductivity. The proposed experiments are multi-disciplinary in nature. Importantly, the project was designed, and the proposal largely written, by a Ph.D. candidate. The research proposed here will lead to peer-reviewed publications and provide a base for future studies over the course of an extremely promising scientific career. The project will also support an undergraduate research student at NIU. The PI is heavily involved in science outreach, including classroom visits, museum events and webinars related to evolution and climate change, and is active with NSF-funded outreach activities linked to the ANDRILL and WISSARD programs. He will continue these efforts with this project.", "east": 180.0, "geometry": ["POINT(0 -89.999)"], "keywords": "Antarctica; Biota; Diatom; Marine Sediments; Oceans; Sediment Core; Southern Ocean", "locations": "Antarctica; Southern Ocean", "north": -60.0, "nsf_funding_programs": null, "persons": "Haji-Sheikh, Michael; Scherer, Reed Paul", "project_titles": "Southern Ocean Diatom Taphonomy and Paleoproductivity: A Laboratory Study of Silica Degradation and Export", "projects": [{"proj_uid": "p0000360", "repository": "USAP-DC", "title": "Southern Ocean Diatom Taphonomy and Paleoproductivity: A Laboratory Study of Silica Degradation and Export"}], "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -90.0, "title": "Southern Ocean Diatom Taphonomy and Paleoproductivity: A Laboratory Study of Silica Degradation and Export", "uid": "600127", "west": -180.0}, {"awards": "0739783 Junge, Karen", "bounds_geometry": ["POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))"], "date_created": "Tue, 01 Jan 2013 00:00:00 GMT", "description": "The mechanisms enabling bacteria to be metabolically active at very low temperatures are of considerable importance to polar microbial ecology, astrobiology, climate and cryopreservation. This research program has two main objectives. The first is to investigate metabolic activities and gene expression of polar marine psychrophilic bacteria when confronted with freezing conditions at temperatures above the eutectic of seawater (\u003c54C) to unveil cold adaptation mechanisms with relevance to wintertime sea-ice ecology. The second objective is to discern if psychrophilic processes of leucine incorporation into proteins, shown to occur to -196C, amount to metabolic activity providing for the survival of cells or are merely biochemical reactions still possible in flash-frozen samples without any effect on survival. We will examine extracellular and intracellular processes of psychrophilic activity above and below the eutectic by (i) determining the temperature range of metabolic activities such as DNA synthesis, carbon utilization, respiration and ATP generation using radioactive tracer technology, including a control at liquid helium temperature (-268.9C), (ii) analyzing gene expression in ice using whole genome and microarray analyses and iii) examining the role of exopolymeric substances (EPS) and ice micro-physics for the observed activity using an in-situ microscopy technique. Results of the proposed research can be expected to aid in the determination of cellular and genetic strategies that allow cells to maintain activity at extremely low temperatures within an icy matrix and/or to resume activity again when more growth-permissive conditions are encountered. The research is an interdisciplinary collaboration involving three different institutions with participants in Oceanography, Genomics, and Geophysical Sciences. The proposed activity will support the beginning professional career of a female researcher and will serve as the basis for several undergraduate student laboratory projects.", "east": 180.0, "geometry": ["POINT(0 -89.999)"], "keywords": "Antarctica; Biota; Microbiology; Oceans; Sea Ice; Southern Ocean", "locations": "Southern Ocean; Antarctica", "north": -60.0, "nsf_funding_programs": null, "persons": "Junge, Karen", "project_titles": "Metabolic Activities and Gene Expression of Marine Psychrophiles in Cold Ice", "projects": [{"proj_uid": "p0000673", "repository": "USAP-DC", "title": "Metabolic Activities and Gene Expression of Marine Psychrophiles in Cold Ice"}], "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -90.0, "title": "Metabolic Activities and Gene Expression of Marine Psychrophiles in Cold Ice", "uid": "600083", "west": -180.0}, {"awards": "0440523 Baker, Ian; 9980379 Baker, Ian", "bounds_geometry": ["POINT(-119.516667 -80.016667)", "POINT(-38.466667 72.583333)", "POINT(106.8 -72.466667)"], "date_created": "Mon, 15 Feb 2010 00:00:00 GMT", "description": "This data set contains measurements of impurities and ions in three polar ice cores: the Vostok 5G ice core and the Byrd ice core from Antarctica, and the Greenland Ice Sheet Project 2 (GISP2) D core. Parameters include sample depth, grain size, ion concentration, and ice core impurity information. Measurements were made using Ion Chromatography (IC), optical microscopy, and Scanning Electron Microscope-Energy Dispersive Spectroscopy (SEM/EDS). \n\nData are available via FTP in Microsoft Excel (.xls)and Microsoft Word (.doc) formats.", "east": 106.8, "geometry": ["POINT(-119.516667 -80.016667)", "POINT(-38.466667 72.583333)", "POINT(106.8 -72.466667)"], "keywords": "Antarctica; Arctic; Byrd Glacier; Byrd Ice Core; Chemistry:ice; Chemistry:Ice; Geochemistry; GISP2; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Ice Core Records; Lake Vostok; Paleoclimate; Vostok Ice Core", "locations": "Lake Vostok; Byrd Glacier; Arctic; Antarctica", "north": 72.583333, "nsf_funding_programs": "Antarctic Glaciology", "persons": "Baker, Ian; Obbard, Rachel", "project_titles": "The Physical Properties of the US ITASE Firn and Ice Cores from South Pole to Taylor Dome", "projects": [{"proj_uid": "p0000289", "repository": "USAP-DC", "title": "The Physical Properties of the US ITASE Firn and Ice Cores from South Pole to Taylor Dome"}], "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -80.016667, "title": "Microstructural Location and Composition of Impurities in Polar Ice Cores", "uid": "609436", "west": -119.516667}, {"awards": "0536870 Rogers, Scott", "bounds_geometry": ["POINT(106.8 -72.4667)"], "date_created": "Tue, 01 Jan 2008 00:00:00 GMT", "description": "The large subglacial Lake Vostok in Antarctica is unique ecological site with a novel microbial biota. The temperatures, pressures and lack of light all select for organisms that may not exist anywhere else on Earth. The accretion ice (lake water frozen to the bottom of the lower surface of the glacier) has preserved microbial samples from each region of Lake Vostok as the glacier passes over and into the lake. Thus, without contaminating the lake with microorganisms from the surface, microbes originating from the lake can be collected, transported to the laboratory and studied. Two of the deepest ice cores sections in this project are part of the international allocation. \n\nThe will be shared between four researchers (Sergey Bulat from Russia, Jean-Robert Petit and Daniel Prieur from France, Scott Rogers from USA). The United States team will study, isolate, and characterize bacteria, fungi, and viruses that have been sampled from the lake through the process of ice accretion to the lower surface of 3500+m thick glacier overriding the lake. The project will involve a suite of methods, including molecular, morphological, and cultural. This includes observation and description by fluorescence, light, and electron microscopy, isolation on thirteen separate cultural media, polymerase chain reaction amplification, DNA sequencing, and phylogenetic analyses. Eleven accretion ice core sections, as well as two glacial ice core sections. As well as two glacial ice core sections will be studied. The accretion ice core sections, as well as two glacial ice core sections will be studied. The accretion ice core sections represent all of the major regions of the lake that have been sampled by the accretion process in the vicinity of the Vostok 5G ice core. The broader impacts of the work relate to the impact the results will have on the filed. These long=isolated lakes, deep below the Antarctic ice sheet may contain novel uniquely adapted organisms. Glacial ice contains an enormous diversity of entrapped microbes, some of which may be metabolically active in the ice. The microbes from Lake Vostok are of special interest, since they are adapted to cold, dark, and high pressure. Thus, their enzyme systems and biochemical pathways may be significantly different from those in the microbes that are the subject of current studies. As such, these organisms may form compounds that may have useful applications. Also, study of the accretion ice, and eventually the water, from Lake Vostok will provide a basis for the study of other subglacial lakes. Additionally, study of the microbes in the accretion ice will be useful to those planning to study analogous systems on ice-covered planets and moons.", "east": 106.8, "geometry": ["POINT(106.8 -72.4667)"], "keywords": "Antarctica; Biota; Cryosphere; Glaciers/ice Sheet; Glaciers/Ice Sheet; Glaciology; Hydrothermal Vent; Lake Vostok; Microbes; Subglacial Lake", "locations": "Lake Vostok; Antarctica", "north": -72.4667, "nsf_funding_programs": null, "persons": "Rogers, Scott O.", "project_titles": "Comprehensive Biological Study of Vostok Accretion Ice", "projects": [{"proj_uid": "p0000566", "repository": "USAP-DC", "title": "Comprehensive Biological Study of Vostok Accretion Ice"}], "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -72.4667, "title": "Comprehensive Biological Study of Vostok Accretion Ice", "uid": "600052", "west": 106.8}]

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