{"dp_type": "Project", "free_text": "pyrosequencing"}
[{"awards": "1341429 Ball, Becky", "bounds_geometry": "POLYGON((-68.205783 -60.706633,-65.9444531 -60.706633,-63.6831232 -60.706633,-61.4217933 -60.706633,-59.1604634 -60.706633,-56.8991335 -60.706633,-54.6378036 -60.706633,-52.3764737 -60.706633,-50.1151438 -60.706633,-47.8538139 -60.706633,-45.592484 -60.706633,-45.592484 -62.1204014,-45.592484 -63.5341698,-45.592484 -64.9479382,-45.592484 -66.3617066,-45.592484 -67.775475,-45.592484 -69.1892434,-45.592484 -70.6030118,-45.592484 -72.0167802,-45.592484 -73.4305486,-45.592484 -74.844317,-47.8538139 -74.844317,-50.1151438 -74.844317,-52.3764737 -74.844317,-54.6378036 -74.844317,-56.8991335 -74.844317,-59.1604634 -74.844317,-61.4217933 -74.844317,-63.6831232 -74.844317,-65.9444531 -74.844317,-68.205783 -74.844317,-68.205783 -73.4305486,-68.205783 -72.0167802,-68.205783 -70.6030118,-68.205783 -69.1892434,-68.205783 -67.775475,-68.205783 -66.3617066,-68.205783 -64.9479382,-68.205783 -63.5341698,-68.205783 -62.1204014,-68.205783 -60.706633))", "dataset_titles": "Climatic and environmental constraints on aboveground-belowground linkages and diversity across a latitudinal gradient in Antarctica", "datasets": [{"dataset_uid": "200289", "doi": "", "keywords": null, "people": null, "repository": "OSF - Center for Open Science", "science_program": null, "title": "Climatic and environmental constraints on aboveground-belowground linkages and diversity across a latitudinal gradient in Antarctica", "url": "https://osf.io/8xfrc/"}], "date_created": "Thu, 14 Apr 2022 00:00:00 GMT", "description": "The Antarctic Peninsula is experiencing rapid environmental changes, which will influence the community of organisms that live there. However, we know very little about the microscopic organisms living in the soil in this region. Soil biology (including bacteria, fungi, and invertebrates) are responsible for many important processes that sustain ecosystems, such as nutrient recycling. Without understanding the environmental conditions that influence soil biodiversity along the Antarctic Peninsula, our ability to predict the consequences of global change is strongly limited. This project will identify the soil community at many sites along the Antarctic Peninsula to discover how the community changes with environmental conditions from north to south. The project will also identify how the soil community at each site differs under different types of plants. Understanding more about the ways in which plant cover and climate conditions influence soil biodiversity will allow predictions of how communities will respond to future changes such as climate warming and invasive plant species. The project will also further the NSF goals of making scientific discoveries available to the general public and of training new generations of scientists. The investigators will engage with outreach to K-12 students and the general public both directly and through a blog and will participate in workshops for K-12 teachers. Additionally, the project will provide the opportunity for many undergraduate and graduate students of diverse backgrounds to be trained in interdisciplinary research.\u003cbr/\u003e\u003cbr/\u003eThe investigators will determine the nature and strength of plant-soil linkages in influencing soil community composition and diversity over a latitudinal gradient of environmental and climatic conditions. The goals are to (1) increase our understanding of current biogeography and diversity by providing in-depth knowledge of soil community composition and complexity as it relates to environmental and climatic characteristics; and (2) determine the nature of aboveground-belowground community linkages over varying spatial scales. The team will identify the composition and diversity of soil communities under key habitat types (grass, moss, algae, etc.). Microbial communities (bacteria, fungi, archaea) will be investigated using pyrosequencing for community composition analysis and metagenomic sequencing to identify functional capabilities. Invertebrates (nematodes, tardigrades, rotifers, microarthropods) will be extracted and identified to the lowest possible taxonomic level. Soil chemistry (pH, nutrient content, soil moisture, etc.) and climate conditions will be measured to determine the relationship between soil communities and physical and chemical properties. Structural equation modeling will be used to identify aboveground-belowground linkage pathways and quantify link strengths under varying environmental conditions.", "east": -45.592484, "geometry": "POINT(-56.8991335 -67.775475)", "instruments": null, "is_usap_dc": true, "keywords": "ANIMALS/INVERTEBRATES; SOIL CHEMISTRY; BACTERIA/ARCHAEA; FUNGI; FIELD INVESTIGATION; Antarctic Peninsula; AMD; ECOSYSTEM FUNCTIONS; USAP-DC; AMD/US; TERRESTRIAL ECOSYSTEMS; USA/NSF", "locations": "Antarctic Peninsula", "north": -60.706633, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Ball, Becky; Van Horn, David", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION", "repo": "OSF - Center for Open Science", "repositories": "Other", "science_programs": null, "south": -74.844317, "title": "Collaborative Research: Climatic and Environmental Constraints on Aboveground-Belowground Linkages and Diversity across a Latitudinal Gradient in Antarctica", "uid": "p0010314", "west": -68.205783}, {"awards": "1141936 Foreman, Christine", "bounds_geometry": "POINT(112.085 -79.467)", "dataset_titles": "Molecular Level Characterization of Dissolved Organic Carbon and Microbial Diversity in the WAIS Divide Replicate Core", "datasets": [{"dataset_uid": "600133", "doi": "10.15784/600133", "keywords": "Antarctica; Biology; Biosphere; Cryosphere; Genetic Sequences; Glaciers/Ice Sheet; Glaciology; Ice Core Records; Paleoclimate; WAIS divide; WAIS Divide Ice Core", "people": "Foreman, Christine", "repository": "USAP-DC", "science_program": "WAIS Divide Ice Core", "title": "Molecular Level Characterization of Dissolved Organic Carbon and Microbial Diversity in the WAIS Divide Replicate Core", "url": "https://www.usap-dc.org/view/dataset/600133"}], "date_created": "Thu, 05 Nov 2015 00:00:00 GMT", "description": "This award supports a detailed, molecular level characterization of dissolved organic carbon and microbes in Antarctic ice cores. Using the most modern biological (genomic), geochemical techniques, and advanced chemical instrumentation researchers will 1) optimize protocols for collecting, extracting and amplifying DNA from deep ice cores suitable for use in next generation pyrosequencing; 2) determine the microbial diversity within the ice core; and 3) obtain and analyze detailed molecular characterizations of the carbon in the ice by ultrahigh resolution Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS). With this pilot study investigators will be able to quantify the amount of material (microbial biomass and carbon) required to perform these characterizations, which is needed to inform future ice coring projects. The ultimate goal will be to develop protocols that maximize the yield, while minimizing the amount of ice required. The broader impacts include education and outreach at both the local and national levels. As a faculty mentor with the American Indian Research Opportunities and BRIDGES programs at Montana State University, Foreman will serve as a mentor to a Native American student in the lab during the summer months. Susan Kelly is an Education and Outreach Coordinator with a MS degree in Geology and over 10 years of experience in science outreach. She will coordinate efforts for comprehensive educational collaboration with the Hardin School District on the Crow Indian Reservation in South-central Montana.", "east": 112.085, "geometry": "POINT(112.085 -79.467)", "instruments": "IN SITU/LABORATORY INSTRUMENTS \u003e CHEMICAL METERS/ANALYZERS \u003e ADS; IN SITU/LABORATORY INSTRUMENTS \u003e CORERS \u003e CORING DEVICES; IN SITU/LABORATORY INSTRUMENTS \u003e SPECTROMETERS/RADIOMETERS \u003e MASS SPECTROMETERS", "is_usap_dc": true, "keywords": "LABORATORY; Dissolved Organic Carbon; WAIS divide; FIELD SURVEYS; Microbes; Ice Core; Not provided; molecular; Antarctic; FIELD INVESTIGATION; Microbial Diversity; pyrosequencing; DNA", "locations": "Antarctic; WAIS divide", "north": -79.467, "nsf_funding_programs": "Antarctic Glaciology", "paleo_time": null, "persons": "Foreman, Christine", "platforms": "LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD SURVEYS; OTHER \u003e PHYSICAL MODELS \u003e LABORATORY; LAND-BASED PLATFORMS \u003e FIELD SITES \u003e FIELD INVESTIGATION; Not provided", "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": "WAIS Divide Ice Core", "south": -79.467, "title": "Molecular Level Characterization of Dissolved Organic Carbon and Microbial Diversity in the WAIS Divide Replicate Core", "uid": "p0000342", "west": 112.085}]
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Project Title/Abstract/Map | NSF Award(s) | Date Created | PIs / Scientists | Dataset Links and Repositories | Abstract | Bounds Geometry | Geometry | Selected | Visible | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Collaborative Research: Climatic and Environmental Constraints on Aboveground-Belowground Linkages and Diversity across a Latitudinal Gradient in Antarctica
|
1341429 |
2022-04-14 | Ball, Becky; Van Horn, David |
|
The Antarctic Peninsula is experiencing rapid environmental changes, which will influence the community of organisms that live there. However, we know very little about the microscopic organisms living in the soil in this region. Soil biology (including bacteria, fungi, and invertebrates) are responsible for many important processes that sustain ecosystems, such as nutrient recycling. Without understanding the environmental conditions that influence soil biodiversity along the Antarctic Peninsula, our ability to predict the consequences of global change is strongly limited. This project will identify the soil community at many sites along the Antarctic Peninsula to discover how the community changes with environmental conditions from north to south. The project will also identify how the soil community at each site differs under different types of plants. Understanding more about the ways in which plant cover and climate conditions influence soil biodiversity will allow predictions of how communities will respond to future changes such as climate warming and invasive plant species. The project will also further the NSF goals of making scientific discoveries available to the general public and of training new generations of scientists. The investigators will engage with outreach to K-12 students and the general public both directly and through a blog and will participate in workshops for K-12 teachers. Additionally, the project will provide the opportunity for many undergraduate and graduate students of diverse backgrounds to be trained in interdisciplinary research.<br/><br/>The investigators will determine the nature and strength of plant-soil linkages in influencing soil community composition and diversity over a latitudinal gradient of environmental and climatic conditions. The goals are to (1) increase our understanding of current biogeography and diversity by providing in-depth knowledge of soil community composition and complexity as it relates to environmental and climatic characteristics; and (2) determine the nature of aboveground-belowground community linkages over varying spatial scales. The team will identify the composition and diversity of soil communities under key habitat types (grass, moss, algae, etc.). Microbial communities (bacteria, fungi, archaea) will be investigated using pyrosequencing for community composition analysis and metagenomic sequencing to identify functional capabilities. Invertebrates (nematodes, tardigrades, rotifers, microarthropods) will be extracted and identified to the lowest possible taxonomic level. Soil chemistry (pH, nutrient content, soil moisture, etc.) and climate conditions will be measured to determine the relationship between soil communities and physical and chemical properties. Structural equation modeling will be used to identify aboveground-belowground linkage pathways and quantify link strengths under varying environmental conditions. | POLYGON((-68.205783 -60.706633,-65.9444531 -60.706633,-63.6831232 -60.706633,-61.4217933 -60.706633,-59.1604634 -60.706633,-56.8991335 -60.706633,-54.6378036 -60.706633,-52.3764737 -60.706633,-50.1151438 -60.706633,-47.8538139 -60.706633,-45.592484 -60.706633,-45.592484 -62.1204014,-45.592484 -63.5341698,-45.592484 -64.9479382,-45.592484 -66.3617066,-45.592484 -67.775475,-45.592484 -69.1892434,-45.592484 -70.6030118,-45.592484 -72.0167802,-45.592484 -73.4305486,-45.592484 -74.844317,-47.8538139 -74.844317,-50.1151438 -74.844317,-52.3764737 -74.844317,-54.6378036 -74.844317,-56.8991335 -74.844317,-59.1604634 -74.844317,-61.4217933 -74.844317,-63.6831232 -74.844317,-65.9444531 -74.844317,-68.205783 -74.844317,-68.205783 -73.4305486,-68.205783 -72.0167802,-68.205783 -70.6030118,-68.205783 -69.1892434,-68.205783 -67.775475,-68.205783 -66.3617066,-68.205783 -64.9479382,-68.205783 -63.5341698,-68.205783 -62.1204014,-68.205783 -60.706633)) | POINT(-56.8991335 -67.775475) | false | false | |||
Molecular Level Characterization of Dissolved Organic Carbon and Microbial Diversity in the WAIS Divide Replicate Core
|
1141936 |
2015-11-05 | Foreman, Christine |
|
This award supports a detailed, molecular level characterization of dissolved organic carbon and microbes in Antarctic ice cores. Using the most modern biological (genomic), geochemical techniques, and advanced chemical instrumentation researchers will 1) optimize protocols for collecting, extracting and amplifying DNA from deep ice cores suitable for use in next generation pyrosequencing; 2) determine the microbial diversity within the ice core; and 3) obtain and analyze detailed molecular characterizations of the carbon in the ice by ultrahigh resolution Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS). With this pilot study investigators will be able to quantify the amount of material (microbial biomass and carbon) required to perform these characterizations, which is needed to inform future ice coring projects. The ultimate goal will be to develop protocols that maximize the yield, while minimizing the amount of ice required. The broader impacts include education and outreach at both the local and national levels. As a faculty mentor with the American Indian Research Opportunities and BRIDGES programs at Montana State University, Foreman will serve as a mentor to a Native American student in the lab during the summer months. Susan Kelly is an Education and Outreach Coordinator with a MS degree in Geology and over 10 years of experience in science outreach. She will coordinate efforts for comprehensive educational collaboration with the Hardin School District on the Crow Indian Reservation in South-central Montana. | POINT(112.085 -79.467) | POINT(112.085 -79.467) | false | false |