[{"awards": "2207011 Granger, Julie", "bounds_geometry": "POLYGON((-180 -60,-144 -60,-108 -60,-72 -60,-36 -60,0 -60,36 -60,72 -60,108 -60,144 -60,180 -60,180 -63,180 -66,180 -69,180 -72,180 -75,180 -78,180 -81,180 -84,180 -87,180 -90,144 -90,108 -90,72 -90,36 -90,0 -90,-36 -90,-72 -90,-108 -90,-144 -90,-180 -90,-180 -87,-180 -84,-180 -81,-180 -78,-180 -75,-180 -72,-180 -69,-180 -66,-180 -63,-180 -60))", "dataset_titles": "Phytoplankton growth rates with siderophore and phytic acid", "datasets": [{"dataset_uid": "601929", "doi": null, "keywords": "Antarctica; Cryosphere; Diatom; Phytoplankton; Siderophore", "people": "Granger, Julie", "repository": "USAP-DC", "science_program": null, "title": "Phytoplankton growth rates with siderophore and phytic acid", "url": "https://www.usap-dc.org/view/dataset/601929"}], "date_created": "Mon, 04 Mar 2024 00:00:00 GMT", "description": "Phytoplankton are microscopic single-celled plants that grow at the sun-lit surface of the ocean. In the Southern Ocean around Antarctica, phytoplankton live in sub-optimal conditions because the amount of iron in seawater is insufficient for growth. Moreover, the chemical composition of Southern Ocean phytoplankton is distinct from that in other ocean regions, with a higher proportion of phosphorus relative to other elements, a characteristic that ultimately influences the distribution of nutrients ocean-wide. The researchers hypothesize that the high phosphorus composition of phytoplankton in the Southern Ocean is caused by their low iron content. Specifically, they postulate that a phosphorus-rich molecule, phytic acid, is synthesized by phytoplankton in order to assist in the storage of iron in designated cellular compartments, such as vacuoles. Recent observations show that some phytoplankton can absorb phytic acid, suggesting that it may be produced by certain species. Phytic acid is pervasive in soils, wherein it aids absorption of iron via plant roots and could similarly help phytoplankton in the Southern Ocean acquire iron via the cell membranes. This project benefits the National Science Foundation\u0027s goals of improving understanding of interactions between the Southern Ocean and the global ocean, of expanding fundamental knowledge of Antarctic biota and associated processes by focusing on phytoplankton species unique to the Antarctic. As part of this project, the Department of Marine Sciences from the College of Liberal Arts and Sciences at the University of Connecticut will sponsor the recruitment, relocation and mentorship of a graduate student under-represented in the sciences. This project aims to determine whether the unusual elemental composition of phytoplankton at the Southern Ocean is a result of anemia. The work will query whether inositol hexakisphosphate (phytic acid) aids Antarctic phytoplankton acquire and store iron, resulting in an elevated fraction of cellular phosphorus relative to other elements. The researchers, including a graduate student, will conduct laboratory culture experiments with phytoplankton strains isolated from the Southern Ocean. They will grow cells in iron- deficient versus iron-replete media to see if their phosphorus content is higher in iron-deficient conditions. They will test whether cells grown with sufficient phosphorus acquire more iron, allowing them to grow better in iron-deficient conditions than cells deriving from phosphorus-poor conditions. They will also query whether cells grown in iron-deficient conditions achieve faster growth rates in the presence of phytic acid. Results will inform the design of CRISPR mutants with which to investigate phosphorus and iron co-metabolism in Antarctic marine phytoplankton. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": 180.0, "geometry": "POINT(0 -89.999)", "instruments": null, "is_usap_dc": true, "keywords": "Dinoflagellates; Iron; United States Of America; Iron Acquisition; Siderophore; TRACE ELEMENTS; Iron Limitation", "locations": "United States Of America", "north": -60.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Granger, Julie; Lin, Senjie", "platforms": null, "repo": "USAP-DC", "repositories": "USAP-DC", "science_programs": null, "south": -90.0, "title": "Siderophore utilization by dinoflagellates as a strategy for iron acquisition", "uid": "p0010455", "west": -180.0}, {"awards": "1744767 Sanders, Robert", "bounds_geometry": "POLYGON((-68 -64,-67.4 -64,-66.8 -64,-66.2 -64,-65.6 -64,-65 -64,-64.4 -64,-63.8 -64,-63.2 -64,-62.6 -64,-62 -64,-62 -64.5,-62 -65,-62 -65.5,-62 -66,-62 -66.5,-62 -67,-62 -67.5,-62 -68,-62 -68.5,-62 -69,-62.6 -69,-63.2 -69,-63.8 -69,-64.4 -69,-65 -69,-65.6 -69,-66.2 -69,-66.8 -69,-67.4 -69,-68 -69,-68 -68.5,-68 -68,-68 -67.5,-68 -67,-68 -66.5,-68 -66,-68 -65.5,-68 -65,-68 -64.5,-68 -64))", "dataset_titles": "Companion datasets to Diversity of microbial eukaryotes along the West Antarctic peninsula in austral spring.; Expedition Data of NBP1910; Expedition Data of NBP 2205; LMG1904 expedition data; NBP1910_protist_community_RNA Raw sequence reads; NBP2205_protist_community_RNA Raw sequence reads will be made available here after processing is completed", "datasets": [{"dataset_uid": "200325", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data of NBP1910", "url": "https://www.rvdata.us/search/cruise/NBP1910"}, {"dataset_uid": "200365", "doi": "", "keywords": null, "people": null, "repository": "NCBI", "science_program": null, "title": "NBP1910_protist_community_RNA Raw sequence reads; NBP2205_protist_community_RNA Raw sequence reads will be made available here after processing is completed", "url": "https://www.ncbi.nlm.nih.gov/search/all/?term=PRJNA807326"}, {"dataset_uid": "200320", "doi": "10.6084/m9.figshare.19514110.v3", "keywords": null, "people": null, "repository": "Figshare", "science_program": null, "title": "Companion datasets to Diversity of microbial eukaryotes along the West Antarctic peninsula in austral spring.", "url": "https://doi.org/10.6084/m9.figshare.19514110.v3"}, {"dataset_uid": "200366", "doi": null, "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "Expedition Data of NBP 2205", "url": "https://www.rvdata.us/search/cruise/NBP2205"}, {"dataset_uid": "200147", "doi": "10.7284/908260", "keywords": null, "people": null, "repository": "R2R", "science_program": null, "title": "LMG1904 expedition data", "url": "https://www.rvdata.us/search/cruise/LMG1904"}], "date_created": "Wed, 27 Jul 2022 00:00:00 GMT", "description": "Traditional models of oceanic food chains have consisted of photosynthetic algae (phytoplankton) being ingested by small animals (zooplankton), which were ingested by larger animals (fish). These traditional models changed as new methods allowed recognition of the importance of bacteria and other non-photosynthetic protozoa in more complex food webs. More recently, the wide-spread existence of mixotrophs (organisms that can both photosynthesize and ingest food particles) and their importance as microbial predators has been recognized in many oceanographic areas. In the Southern Ocean, the only two surveys of mixotrophs have suggested that there may be seasonal differences in their importance as predators. During the long polar night (winter), the ability of mixotrophs to ingest particulate food may aid in their survival thus ensuring a sufficient population in spring to support a phytoplankton bloom once photosynthesis rates can increase. Thus mixotrophs may provide a critical early food source upon which zooplankton and larger animals depend on for growth and reproduction. This project will advance understanding of mixotroph diversity and their ecological impact within the Southern Ocean microbial food web. Specifically, efforts will be focused on mixotrophy in the western Antarctica peninsula region during the austral spring and autumn when there are likely to be changes in the relative importance of photosynthesis and ingestion to mixotrophs. The project will provide research opportunities for undergraduate and graduate students and a post-doctoral researcher. There will be real-time outreach from the Southern Ocean to the public via blogs and interviews, and to high school art students through an established program that blends science and art education. Despite traditional views of protists as either \"phototrophic\" or \"heterotrophic,\" there are many photosynthetic protists that consume prey (mixotrophy). Mixotrophy is a widespread phenomenon in aquatic systems and phytoplankton groups with known mixotrophic species, notably chrysophytes, cryptophytes, prymnesiophytes, prasinophytes and dinoflagellates, are present and often abundant in Antarctic waters. However, in the Southern Ocean, the presence of mixotrophic phytoflagellates has been surveyed only twice: in the Ross Sea during Austral spring 2008 and summer 2011. The primary goals of the project are to gain better understanding of mixotroph diversity and their ecological impact with respect to the Southern Ocean microbial food web. The contribution of mixotrophs to primary production and bacterial consumption is likely linked to the taxonomic composition of the community and the abundance of particular species. Abundances of novel mixotrophic species will be evaluated via qPCR, which will be coupled with assessments of rates of feeding and photosynthesis with the goal of describing how active mixotrophs direct the movement of carbon through food webs. These experiments will help the determination of how viable and widespread mixotrophy is as a nutritional strategy in polar waters and give direct information on the currently unknown diversity of mixotrophic taxa under different environmental conditions occurring in austral spring and autumn. Furthermore, the methods will simultaneously yield information on the whole communities of protists - mixotrophic, phototrophic and heterotrophic. In addition, a method to examine aspects of the taxonomic and functional diversities of the bacterivorous/mixotrophic community will be employed. A thymidine analog (BrdU) will be used to label DNA of eukaryotes feeding on bacteria. The BrdU-labeled eukaryotic DNA will be isolated using immunoprecipitation. High-throughput sequencing of the labeled DNA (bacterivores) versus unlabeled community DNA will determine the diversity of bacterivorous mixotrophs relative to other microeukaryotes. Flow cytometric sorting based on chlorophyll to focus on mixotrophic species. These approaches will elucidate a gap in current knowledge of the influence of microbial interactions in the Southern Ocean under different conditions. This award reflects NSF\u0027s statutory mission and has been deemed worthy of support through evaluation using the Foundation\u0027s intellectual merit and broader impacts review criteria.", "east": -62.0, "geometry": "POINT(-65 -66.5)", "instruments": null, "is_usap_dc": true, "keywords": "Antarctic Peninsula; PLANKTON; COASTAL", "locations": "Antarctic Peninsula", "north": -64.0, "nsf_funding_programs": "Antarctic Organisms and Ecosystems", "paleo_time": null, "persons": "Sanders, Robert; Gast, Rebecca; Jeffrey, Wade H.", "platforms": null, "repo": "R2R", "repositories": "Figshare; NCBI; R2R", "science_programs": null, "south": -69.0, "title": "Collaborative Research: Diversity and ecological impacts of Antarctic mixotrophic phytoplankton", "uid": "p0010357", "west": -68.0}]

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Siderophore utilization by dinoflagellates as a strategy for iron acquisition

2207011

2024-03-04

Granger, Julie; Lin, Senjie

Phytoplankton growth rates with siderophore and phytic acid

USAP-DC

Phytoplankton are microscopic single-celled plants that grow at the sun-lit surface of the ocean. In the Southern Ocean around Antarctica, phytoplankton live in sub-optimal conditions because the amount of iron in seawater is insufficient for growth. Moreover, the chemical composition of Southern Ocean phytoplankton is distinct from that in other ocean regions, with a higher proportion of phosphorus relative to other elements, a characteristic that ultimately influences the distribution of nutrients ocean-wide. The researchers hypothesize that the high phosphorus composition of phytoplankton in the Southern Ocean is caused by their low iron content. Specifically, they postulate that a phosphorus-rich molecule, phytic acid, is synthesized by phytoplankton in order to assist in the storage of iron in designated cellular compartments, such as vacuoles. Recent observations show that some phytoplankton can absorb phytic acid, suggesting that it may be produced by certain species. Phytic acid is pervasive in soils, wherein it aids absorption of iron via plant roots and could similarly help phytoplankton in the Southern Ocean acquire iron via the cell membranes. This project benefits the National Science Foundation's goals of improving understanding of interactions between the Southern Ocean and the global ocean, of expanding fundamental knowledge of Antarctic biota and associated processes by focusing on phytoplankton species unique to the Antarctic. As part of this project, the Department of Marine Sciences from the College of Liberal Arts and Sciences at the University of Connecticut will sponsor the recruitment, relocation and mentorship of a graduate student under-represented in the sciences. This project aims to determine whether the unusual elemental composition of phytoplankton at the Southern Ocean is a result of anemia. The work will query whether inositol hexakisphosphate (phytic acid) aids Antarctic phytoplankton acquire and store iron, resulting in an elevated fraction of cellular phosphorus relative to other elements. The researchers, including a graduate student, will conduct laboratory culture experiments with phytoplankton strains isolated from the Southern Ocean. They will grow cells in iron- deficient versus iron-replete media to see if their phosphorus content is higher in iron-deficient conditions. They will test whether cells grown with sufficient phosphorus acquire more iron, allowing them to grow better in iron-deficient conditions than cells deriving from phosphorus-poor conditions. They will also query whether cells grown in iron-deficient conditions achieve faster growth rates in the presence of phytic acid. Results will inform the design of CRISPR mutants with which to investigate phosphorus and iron co-metabolism in Antarctic marine phytoplankton. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

Collaborative Research: Diversity and ecological impacts of Antarctic mixotrophic phytoplankton

1744767

2022-07-27

Sanders, Robert; Gast, Rebecca; Jeffrey, Wade H.

Expedition Data of NBP1910	R2R
NBP1910_protist_community_RNA Raw sequence reads; NBP2205_protist_community_RNA Raw sequence reads will be made available here after processing is completed	NCBI
Companion datasets to Diversity of microbial eukaryotes along the West Antarctic peninsula in austral spring.	Figshare
Expedition Data of NBP 2205	R2R
LMG1904 expedition data	R2R

Traditional models of oceanic food chains have consisted of photosynthetic algae (phytoplankton) being ingested by small animals (zooplankton), which were ingested by larger animals (fish). These traditional models changed as new methods allowed recognition of the importance of bacteria and other non-photosynthetic protozoa in more complex food webs. More recently, the wide-spread existence of mixotrophs (organisms that can both photosynthesize and ingest food particles) and their importance as microbial predators has been recognized in many oceanographic areas. In the Southern Ocean, the only two surveys of mixotrophs have suggested that there may be seasonal differences in their importance as predators. During the long polar night (winter), the ability of mixotrophs to ingest particulate food may aid in their survival thus ensuring a sufficient population in spring to support a phytoplankton bloom once photosynthesis rates can increase. Thus mixotrophs may provide a critical early food source upon which zooplankton and larger animals depend on for growth and reproduction. This project will advance understanding of mixotroph diversity and their ecological impact within the Southern Ocean microbial food web. Specifically, efforts will be focused on mixotrophy in the western Antarctica peninsula region during the austral spring and autumn when there are likely to be changes in the relative importance of photosynthesis and ingestion to mixotrophs. The project will provide research opportunities for undergraduate and graduate students and a post-doctoral researcher. There will be real-time outreach from the Southern Ocean to the public via blogs and interviews, and to high school art students through an established program that blends science and art education. Despite traditional views of protists as either "phototrophic" or "heterotrophic," there are many photosynthetic protists that consume prey (mixotrophy). Mixotrophy is a widespread phenomenon in aquatic systems and phytoplankton groups with known mixotrophic species, notably chrysophytes, cryptophytes, prymnesiophytes, prasinophytes and dinoflagellates, are present and often abundant in Antarctic waters. However, in the Southern Ocean, the presence of mixotrophic phytoflagellates has been surveyed only twice: in the Ross Sea during Austral spring 2008 and summer 2011. The primary goals of the project are to gain better understanding of mixotroph diversity and their ecological impact with respect to the Southern Ocean microbial food web. The contribution of mixotrophs to primary production and bacterial consumption is likely linked to the taxonomic composition of the community and the abundance of particular species. Abundances of novel mixotrophic species will be evaluated via qPCR, which will be coupled with assessments of rates of feeding and photosynthesis with the goal of describing how active mixotrophs direct the movement of carbon through food webs. These experiments will help the determination of how viable and widespread mixotrophy is as a nutritional strategy in polar waters and give direct information on the currently unknown diversity of mixotrophic taxa under different environmental conditions occurring in austral spring and autumn. Furthermore, the methods will simultaneously yield information on the whole communities of protists - mixotrophic, phototrophic and heterotrophic. In addition, a method to examine aspects of the taxonomic and functional diversities of the bacterivorous/mixotrophic community will be employed. A thymidine analog (BrdU) will be used to label DNA of eukaryotes feeding on bacteria. The BrdU-labeled eukaryotic DNA will be isolated using immunoprecipitation. High-throughput sequencing of the labeled DNA (bacterivores) versus unlabeled community DNA will determine the diversity of bacterivorous mixotrophs relative to other microeukaryotes. Flow cytometric sorting based on chlorophyll to focus on mixotrophic species. These approaches will elucidate a gap in current knowledge of the influence of microbial interactions in the Southern Ocean under different conditions. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.