IEDA
Project Information
Collaborative Research: Winter Survival Mechanisms and Adaptive Genetic Variation in an Antarctic Insect
Start Date:
2014-08-15
End Date:
2018-07-31
Project Location(s)
Palmer Station and Vacinity
Description/Abstract
Polar regions are deserts that are not only cold but also lack access to free water. Antarctic insects have unique survival mechanisms including the ability to tolerate freezing and extensive dehydration, surviving the loss of 70% of their body water. How this is done is of interest not only for understanding seasonal adaptations of insects and how they respond to climate change, but the molecular and physiological mechanisms employed may offer valuable insights into more general mechanisms that might be exploited for cryopreservation and long-term storage of human tissues and organs for transplantation and other medical applications. The investigators will study the proteins that are responsible for removing water from the body, cell level consequences of this, and how the responsible genes vary between populations. The project will also further the NSF goals of making scientific discoveries available to the general public and of training new generations of scientists. Each year a K-12 teacher will be a member of the field team and assist with fieldwork and outreach to school children and their teachers. Educational outreach efforts include presentations at local schools and national teacher meetings, providing lesson plans and podcasts on a website, and continuing to publish articles related to this research in education journals. In addition, undergraduate and graduate students will receive extensive training in all aspects of the research project with extended experiences that include publication of scientific papers and presentations at national meetings.

This project focuses on deciphering the physiological and molecular mechanisms that enable the Antarctic midge Belgica antarctica to survive environmental stress and the loss of most of its body water in the desiccating polar environment. This extremophile is an ideal system for investigating mechanisms of stress tolerance and local geographic adaptations and its genome has recently been sequenced. This project has three focal areas: 1) Evaluating the role of aquaporins (water channel proteins) in the rapid removal of water from the body by studying expression of their genes during dehydration; 2) Investigating the mechanism of metabolic depression and the role of autophagy (controlled breakdown of cellular components) as a mediator of stress tolerance by studying expression of the genes responsible for autophagy during the dehydration process; and 3) Evaluating the population structure, gene flow, and adaptive variation in physiological traits associated with stress tolerance using a genetic approach that takes advantage of the genomic sequence available for this species coupled with physiological and environmental data from the sampled populations and their habitats.
Personnel
Person Role
Denlinger, David Investigator and contact
Lee, Richard Investigator
Funding
Antarctic Organisms and Ecosystems Award # 1341393
Antarctic Organisms and Ecosystems Award # 1341385
AMD - DIF Record(s)
Data Management Plan
None in the Database
Publications
  1. Spacht, D.E., N.M. Teets and D.L. Denlinger. 2018. Two isoforms of Pepck in Sarcophaga bullata and their distinct expression profiles through development, diapause, and in response to stresses of cold and starvation. Journal of Insect Physiology 111:41-46. (doi:10.1016/j.jinsphys.2018.10.008)
  2. Meibers, H.E., G. Finch, R.T. Gregg, S. Glenn, K.D. Assani, E.C. Jennings, B. Davies, A.J. Rosendale, C.J. Holmes, J.D.Gantz, D.E. Spacht, R.E. Lee, Jr., D.L. Denlinger, M.T. Weirauch and J.B. Benoit. 2019. Sex- and developmental-specific transcriptomic analyses of the Antarctic mite, Alaskozetes antarcticus, reveal transcriptional shifts underlying oribatid mite reproduction. Polar Biology 42:357-370. (doi:10.1007/s00300-018-2427-x)
  3. Kawarasaki, Y., N.M. Teets, B.N. Philip, L.J. Potts, J.D. Gantz, D.L. Denlinger and R.E. Lee, Jr. 2019. Characterization of drought-induced rapid cold-hardening in the Antarctic midge, Belgica antarctica. Polar Biology 42: 1147-1156. (doi:10.1007/s00300-019-02503-6)
  4. Holmes, C.J., E.C. Jennings, J.D. Gantz, D. Spacht, A.A. Spangler, D.L. Denlinger, R.E. Lee, Jr., T.L. Hamilton and J.B. Benoit. 2019. The Antarctic mite, Alaskozetes antarcticus, shares bacterial microbiome community membership but not abundance between adults and tritonymphs. Polar Biology (submitted).
  5. Teets, N.M., Y. Kawarasaki, L.J. Potts, B.N. Philip, J.D. Ganz, D.L. Denlinger and R.E. Lee. Rapid cold hardening protects against sublethal freezing injury in an Antarctic insect. Journal of Experimental Biology 222, jeb206011. (doi:10.1242/jeb.206011)