USAP-DC

The proposed research will investigate the interacting and potentially synergistic influence of two oceanographic features - ocean acidification and the projected rise in mean sea surface temperature - on the performance of Notothenioids, the dominant fish of the Antarctic marine ecosystem. Understanding the joint effects of acidification and temperature rise on these fish is a vital component of predicting the resilience of coastal marine ecosystems. Notothenioids have repeatedly displayed a narrow window of physiological tolerances when subjected to abiotic stresses. Given that evolutionary adaptation may have led to finely-tuned traits with narrow physiological limits in these organisms, this system provides a unique opportunity to examine physiological trade-offs associated with acclimation to the multi-stressor environment expected from future atmospheric CO2 projections. Understanding these trade-offs will provide valuable insight into the capacity species have for responses to climate change via phenotypic plasticity. As an extension to functional measurements, this study will use evolutionary approaches to map variation in physiological responses onto the phylogeny of these fishes and the genetic diversity within species. These approaches offer insight into the historical constraints and future potential for evolutionary optimization. The research will significantly expand the genomic resources available to polar researchers and will support the training of graduate students and a post doc at an EPSCoR institution. Research outcomes will be incorporated into classroom curriculum.

Person	Role
Place, Sean	Investigator
Sarmiento, Jorge	Investigator
Dudycha, Jeffry	Co-Investigator
Kwon, Eun-Young	Co-Investigator

Antarctic Organisms and Ecosystems	Award # 1447291
Antarctic Integrated System Science	Award # 1040957
Antarctic Organisms and Ecosystems	Award # 1040957
Antarctic Organisms and Ecosystems	Award # 1040945

NSF-ANT10-40957
NSF-ANT10-40945
NSF-ANT14-47291

None in the Database

Repository	Title (link)	Format(s)	Status
NCBI GenBank	NCBI GenBank RNA sequences, Trematomus bernacchii	None	exist
NCBI GenBank	NCBI GenBank RNA sequences, Pagothenia borchgrevinki	None	exist
NCBI GenBank	NCBI GenBank RNA sequences, Trematomus newnesi	None	exist
BCO-DMO	NCBI links to BioProjects of total RNA isolated from Trematomus bernacchii gill tissues acclimated to elevated temperature and pCO2, July 2015	None	exist
NCBI GenBank	NCBI GenBank RNA sequences, Trematomus bernacchii	None	exist
NCBI GenBank	NCBI GenBank RNA sequences, Pagothenia borchgrevinki	None	exist
NCBI GenBank	NCBI GenBank RNA sequences, Trematomus newnesi	None	exist
BCO-DMO	Does the strength of the carbonate pump change with ocean stratification and acidification and how? Project data	None	exist

1. Vasadia, D.J., Zippay, M.L., Place, S.P. (2019). Characterization of thermally sensitive miRNAs reveals a central role of the FoxO signaling pathway in regulating the cellular stress response of an extreme stenotherm, Trematomus bernacchii. Marine Genomics. (doi:10.1016/j.margen.2019.100698)
2. Enzor, L.A., Hunter, E.M. and S.P. Place (2017). The effects of elevated temperature and ocean acidification on the metabolic pathways of notothenioid fish. Conserv Physiol. 5 (1): cox019. (doi:10.1093/conphys/cox019)
3. Huth, T. J., & Place, S. P. (2016). RNA-seq reveals a diminished acclimation response to the combined effects of ocean acidification and elevated seawater temperature in Pagothenia borchgrevinki. Marine Genomics, 28, 87–97. (doi:10.1016/j.margen.2016.02.004)
4. Huth, T. J., & Place, S. P. (2016). Transcriptome wide analyses reveal a sustained cellular stress response in the gill tissue of Trematomus bernacchii after acclimation to multiple stressors. BMC Genomics, 17(1). (doi:10.1186/s12864-016-2454-3)
5. Enzor, L. A., Zippay, M. L., & Place, S. P. (2013). High latitude fish in a high CO2 world: Synergistic effects of elevated temperature and carbon dioxide on the metabolic rates of Antarctic notothenioids. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 164(1), 154–161. (doi:10.1016/j.cbpa.2012.07.016)
6. Carter, B. R., Talley, L. D., & Dickson, A. G. (2014). Mixing and remineralization in waters detrained from the surface into Subantarctic Mode Water and Antarctic Intermediate Water in the southeastern Pacific. Journal of Geophysical Research: Oceans, 119(6), 4001–4028. (doi:10.1002/2013jc009355)
7. Bogan, S. N., & Place, S. P. (2019). Accelerated evolution at chaperone promoters among Antarctic notothenioid fishes. BMC Evolutionary Biology, 19(1). (doi:10.1186/s12862-019-1524-y)