IEDA
Metadata
Resource Type
Dataset
Data DOI
AMD - DIF Record(s)
Repository (current):
USAP-DC
Title
UPLC-Q-TOF data of Cotton Glacier exometabolites
Abstract
Mass spectra of external metabolites were obtained with a 1290 Ultra Performance Liquid Chromatography system coupled to a 6538 Ultra High Definition Accurate-Mass Quadrupole-Time of Flight mass spectrometer operated in positive mode with an electrospray ionization source (Agilent Technologies). 30 mL of filtered media was concentrated per sample by solid phase extraction. External metabolites were re-suspended in 50% (v/v) acetonitrile, and were separated using a reverse-phase Kinetix 1.7 um C18, 100A, 150 mm - 2.1 mm column. Data presented are from UPLC-Q-TOF measurements of mass to charge ratio, retention time, and replicate-averaged extracted ion chromatogram abundance values (counts) of molecular species that demonstrated a significant change in abundance (Two-way ANOVA, adjusted P<0.01) during incubations based on time point (T0: d0, T1: d27, T2: d63, T3: d98) and carbon source (Cotton Glacier: CG, Pony Lake: PL, Suwannee River: SR).
Date Created
2018-03-23
Creator(s)
Michelle Tigges, Brian Bothner and Christine Foreman
Spatial Extent(s)
West: 161.1667
East: 161.667
South: -77.117
North: -77.117
Temporal Extent(s)
Start: 2013-10-15
End: 2014-01-22
Award
Version
1
Language
English
References
  1. Dynamic processing of DOM: Insight from Exometabolomics, Fluorescence Spectroscopy, and Mass Spectrometry. Limnology and Oceanography Letters 2018
Supplemental Docs
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README_601089.txt
Data Files
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142.0 kB
LCQTOF_spectrometer_data.xlsx