IEDA
Dataset Information
UPLC-Q-TOF data of Cotton Glacier exometabolites
Data DOI:
https://doi.org/10.15784/601089
Cite as
Tigges, M., Bothner, B., & Foreman, C. (2018) "UPLC-Q-TOF data of Cotton Glacier exometabolites" U.S. Antarctic Program (USAP) Data Center. doi: https://doi.org/10.15784/601089.
AMD - DIF Record(s)
Abstract
Mass spectra of external metabolites were obtained with a 1290 Ultra Performance Liquid Chromatography system coupled to a 6538 Ultra High Definition Accurate-Mass Quadrupole-Time of Flight mass spectrometer operated in positive mode with an electrospray ionization source (Agilent Technologies). 30 mL of filtered media was concentrated per sample by solid phase extraction. External metabolites were re-suspended in 50% (v/v) acetonitrile, and were separated using a reverse-phase Kinetix 1.7 um C18, 100A, 150 mm - 2.1 mm column. Data presented are from UPLC-Q-TOF measurements of mass to charge ratio, retention time, and replicate-averaged extracted ion chromatogram abundance values (counts) of molecular species that demonstrated a significant change in abundance (Two-way ANOVA, adjusted P<0.01) during incubations based on time point (T0: d0, T1: d27, T2: d63, T3: d98) and carbon source (Cotton Glacier: CG, Pony Lake: PL, Suwannee River: SR).
Creator(s):
Tigges, Michelle; Bothner, Brian; Foreman, Christine
Date Created:
2018-03-23
Repository:
USAP-DC (current)
Spatial Extent(s)
West: 161.1667, East: 161.667, South: -77.117, North: -77.117
Temporal Extent(s)
Start: 2013-10-15 - End: 2014-01-22
Award(s)
Version:
1
References
  1. Smith, H. J., Tigges, M. , D'Andrilli, J. , Parker, A. , Bothner, B. and Foreman, C. M. (2018), Dynamic processing of DOM: Insight from exometabolomics, fluorescence spectroscopy, and mass spectrometry. Limnol. Oceanogr., 3: 225-235 (doi:10.1002/lol2.10082)
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